CN105806833A - Electrochemical luminescence detection pool - Google Patents
Electrochemical luminescence detection pool Download PDFInfo
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- CN105806833A CN105806833A CN201610292303.4A CN201610292303A CN105806833A CN 105806833 A CN105806833 A CN 105806833A CN 201610292303 A CN201610292303 A CN 201610292303A CN 105806833 A CN105806833 A CN 105806833A
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- 238000000504 luminescence detection Methods 0.000 title abstract 3
- 238000001514 detection method Methods 0.000 claims abstract description 49
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims abstract description 8
- 238000004020 luminiscence type Methods 0.000 claims description 18
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- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 claims description 8
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- JUPQTSLXMOCDHR-UHFFFAOYSA-N benzene-1,4-diol;bis(4-fluorophenyl)methanone Chemical compound OC1=CC=C(O)C=C1.C1=CC(F)=CC=C1C(=O)C1=CC=C(F)C=C1 JUPQTSLXMOCDHR-UHFFFAOYSA-N 0.000 claims description 3
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- -1 bipyridyl ruthenium Chemical compound 0.000 description 7
- 229910052707 ruthenium Inorganic materials 0.000 description 7
- 238000000034 method Methods 0.000 description 5
- BZSVVCFHMVMYCR-UHFFFAOYSA-N 2-pyridin-2-ylpyridine;ruthenium Chemical compound [Ru].N1=CC=CC=C1C1=CC=CC=N1.N1=CC=CC=C1C1=CC=CC=N1.N1=CC=CC=C1C1=CC=CC=N1 BZSVVCFHMVMYCR-UHFFFAOYSA-N 0.000 description 4
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- 239000008363 phosphate buffer Substances 0.000 description 3
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- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 2
- 239000005092 [Ru (Bpy)3]2+ Substances 0.000 description 2
- 238000005251 capillar electrophoresis Methods 0.000 description 2
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- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
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- 229910021607 Silver chloride Inorganic materials 0.000 description 1
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- ZOMNIUBKTOKEHS-UHFFFAOYSA-L dimercury dichloride Chemical class Cl[Hg][Hg]Cl ZOMNIUBKTOKEHS-UHFFFAOYSA-L 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
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- 230000005611 electricity Effects 0.000 description 1
- 238000000840 electrochemical analysis Methods 0.000 description 1
- 238000002848 electrochemical method Methods 0.000 description 1
- 238000001378 electrochemiluminescence detection Methods 0.000 description 1
- 230000005518 electrochemistry Effects 0.000 description 1
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- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/76—Chemiluminescence; Bioluminescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N21/03—Cuvette constructions
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N21/03—Cuvette constructions
- G01N2021/0325—Cells for testing reactions, e.g. containing reagents
Abstract
The invention relates to an electrochemical luminescence detection pool which comprises a detection pool body, a three-electrode system and a solution flow path, wherein a vertical channel and four horizontal channels are arranged in the detection pool body; the vertical channel is formed by a through hole with a quartz glass sheet at bottom; a working electrode, an auxiliary electrode, a sampling steel needle and an effluent pipeline are mounted on the four horizontal channels, respectively; a reference electrode is mounted in the vertical channel; the external end of a sampling pipeline is connected with an injection pump through a pipeline; a pump chamber of the injection pump is communicated with a sample bottle and a buffer solution bottle. According to the invention, a sample is fed into the pipeline through the injection pump, then is pushed into the detection pool by the buffer solution, and is sprayed onto the surface of the working electrode, wherein the suitable pumping speed of the injection pump is selected for ensuring that the sample is wholly sprayed onto the surface of the working electrode. The electrochemical luminescence detection pool is simple in structure and convenient in operation. The stimulation of the whole sample on the surface of the working electrode can be ensured by only adjusting the flowing speed of the injection pump, so that the distance between the tail end of the steel needle and the working electrode does not need to be confirmed by people.
Description
Technical field
The invention belongs to electrochemiluminescence detection technique field, be specifically related to a kind of electrochemical luminescence detecting pool, be a kind of detection cell for carrying out electrochemiluminescence analysis.
Background technology
Electrochemiluminescence is the product that chemiluminescence method be combined with each other with electrochemical method, through 40 years of researches, have become as now a kind of very important analysis method, and be obtained for be widely applied in immunity, food and the field such as water sample test and chemical and biological weapons reagent detection.
Electrochemical luminescence detecting pool is the vitals of electrochemiluminescence analysis, and by applying certain voltage to the electrode in detection cell, the sample in detection cell will occur electrochemiluminescence to react, and discharges the light of certain wavelength.The design of electrochemical luminescence detecting pool is most important, should ensure that sample can be sufficiently carried out electrochemiluminescence reaction, to be also easy to detection cell is carried out.
Current electrochemical luminescence detecting pool is broadly divided into two classes, and one is Static Detection, and one is dynamic detection.Detection cell for Static Detection is fairly simple, it might even be possible to use beaker.But need when carrying out Static Detection to add the electrochemical circuit that substantial amounts of solution is complete to guarantee this System forming, therefore this method is relatively larger for the consumption of sample, is not suitable for micro-example.Dynamically detection is mainly and other method coupling, such as with the coupling such as flow injection or capillary electrophoresis.
During with flow injection coupling, sample solution is to flow through from the direction vertical with working electrode along working electrode surface, and the voltage that working electrode applies causes the reaction of sample generation electrochemiluminescence, and the intensity of the detection system light to discharging detects.Owing to working electrode is positioned at the sidewall of pipeline, therefore the solution in pipeline has different from the distance of working electrode surface.If the distance between the sample segment in solution and working electrode surface is excessive, it is possible to can cause that this sample segment can not obtain being executed on working electrode alive exciting, and testing result is produced impact.
With capillary electrophoresis used time, the end of capillary tube and working electrode level of relative, if distance is suitable, the sample flowed out in capillary tube can flow to the surface of working electrode, and is excited.But the distance between end and the working electrode of capillary tube needs the experience relying on operator to be adjusted, if the distance between capillary end and working electrode surface is improper, then also resulting in sample segment can not be excited.
Summary of the invention
It is an object of the invention to the drawback of the appearance overcoming existing electrochemical luminescence detecting pool in detection process, mainly solve because of apart from improper and that cause sample segment can not be excited problem.
For this, the invention provides a kind of electrochemical luminescence detecting pool, including detection cell cell body, three-electrode system and solution stream, a vertical channel and four horizontal channels connected with vertical channel it are provided with in described detection cell cell body, described vertical channel is through-hole structure, via bottoms is provided with a quartz glass plate, four described horizontal channels include three passages being in same level and a horizontal channel higher than its excess-three passage, it is respectively mounted working electrode in three described passages being in same level, auxiliary electrode and sample introduction draw point, a remaining passage connects waste-solution line, reference electrode is installed in described vertical channel；
Described installation sample introduction draw point pipeline is as sample introduction pipeline, and its outer end connects syringe pump by pipeline, and the pump chamber of syringe pump is respectively communicated with sample bottle and buffer bottle.
Described working electrode is identical with auxiliary electrode, is diameter platinum disk electrode between 0.1～5 millimeter, working electrode and auxiliary electrode distribution at a right angle；Described working electrode is relative with the sample introduction pipeline installing sample introduction draw point；Described waste-solution line is relative with auxiliary electrode and not in the same plane.
It is inner that described sample introduction draw point is arranged on sample introduction pipeline, and sample introduction pipeline is plastic tube；Described sample introduction draw point length is between 1.0～10 centimetres, and pin hole internal diameter is between 0.2～1.0 millimeter；Described waste-solution line is plastic tube.
The material of described detection cell cell body is quartz, glass or lucite.
Described sample introduction draw point, auxiliary electrode, reference electrode, working electrode and waste-solution line are arranged on by joint in the passage of detection cell cell body respectively, the outside of joint is with the screw thread matched with detection cell cell body vias inner walls, and the material of joint is lucite or PEEK.
Beneficial effects of the present invention: this electrochemical luminescence detecting pool provided by the invention, by syringe pump, sample is sent into pipeline, detection cell under the propelling movement of buffer, and it is injected into the surface of working electrode, by selecting the flow velocity of suitable syringe pump, guarantee that sample is all ejected into the surface of working electrode, have the advantage that
1, present configuration is simple, easy to operate；
2, the present invention only need to adjust the flow velocity of syringe pump and namely can ensure that whole sample is all excited at working electrode surface, from without the distance artificially determined between draw point end and working electrode.
Accompanying drawing explanation
Below with reference to accompanying drawing, the present invention is described in further details.
Fig. 1 is the overall structure schematic diagram of the present invention.
Fig. 2 is detection cell schematic top plan view.
Fig. 3 is the A-A sectional view of Fig. 2.
Fig. 4 is the B-B sectional view of Fig. 2.
Fig. 5 is the detection spectrogram of tris (bipyridine) ruthenium sample.
Description of reference numerals: 1, sample introduction draw point；2, auxiliary electrode；3, reference electrode；4, working electrode；5, waste-solution line；6, quartz glass plate；7, syringe pump；8, sample bottle；9, buffer bottle.
Detailed description of the invention
Embodiment 1:
The present embodiment provides a kind of electrochemical luminescence detecting pool, in conjunction with Fig. 1 and Fig. 2, Fig. 3, shown in Fig. 4, including detection cell cell body, three-electrode system and solution stream, a vertical channel and four horizontal channels connected with vertical channel it are provided with in described detection cell cell body, described vertical channel is through-hole structure, via bottoms is provided with a quartz glass plate 6, four described horizontal channels include three passages being in same level and a horizontal channel higher than its excess-three passage, it is respectively mounted working electrode 4 in three described passages being in same level, auxiliary electrode 2 and sample introduction draw point 1, a remaining passage connects waste-solution line 5, reference electrode 3 is installed in described vertical channel；
Installing sample introduction draw point 1 pipeline as sample introduction pipeline, its outer end connects syringe pump 7 by pipeline, and the pump chamber of syringe pump 7 is respectively communicated with sample bottle 8 and buffer bottle 9.
Whole detection cell is placed in the detector of Electrochemial luminescence detecting instrument device, connects each electrode connecting line, be simultaneously connected with each pipeline.After detection starts, sample solution is injected into the surface of working electrode 4 via syringe pump 7 from the sample introduction draw point 1 of sample introduction pipeline, being executed on working electrode 4 surface and alive excite lower generation electrochemiluminescence to react, the intensity of the light that reaction discharges is detected by the photomultiplier tube in instrument.After detection terminates, cleaning buffer solution is entered detection cell by buffer bottle 9 via syringe pump 7, sample introduction pipeline, and detection cell cell body inner chamber is carried out, and waste liquid is flowed out by waste-solution line 5.
Consider the distance between sample introduction draw point 1 and working electrode 4, the surface of working electrode 4 all it is ejected in order to ensure sample, in the present embodiment, require over the pump speed regulating syringe pump 7, can all be excited at working electrode surface by whole samples by regulating the flow velocity of syringe pump 7, from without the distance artificially determined between draw point end and working electrode.
Embodiment 2:
On the basis of embodiment 1, each electrode is further illustrated by the present embodiment.Three electrodes in three-electrode system are working electrode, reference electrode and auxiliary electrode.
Working electrode, also known as Electrode, refers to that the reaction studied occurs on this electrode.Working electrode can be solid, it is also possible to be liquid, and the solid material that can conduct electricity miscellaneous all can serve as electrode.Character generally according to research pre-determines electrode material, but the most common 'inertia' Solid electrode materials is glass carbon (platinum, gold, silver, lead and electro-conductive glass) etc..When adopting solid electrode, in order to ensure the repeatability of experiment, it has to be noted that set up suitable electrode pre-treatment step, to ensure oxidoreduction, surface topography and to be absent from the reproducible state of adsorbing contaminant.In fluid electrode, hydrargyrum and amalgam are the most frequently used working electrodes, and they are all liquid, have reproducible equal phase surface, preparation and maintenance cleaning are all easier to, and the operation window note that on electrode, high hydrogen evolution overpotential improves under nagative potential simultaneously is widely used in electro chemical analysis.
Auxiliary electrode, also known as to electrode, auxiliary electrode and working electrode composition loop, makes working electrode power on smooth logical, to ensure that the reaction studied occurs on the working electrode (s, but must limit the response of battery observation without any mode.When there is oxidation or reduction reaction due to working electrode, auxiliary electrode can be arranged to the evolution reaction of gas or the back reaction of working electrode reaction, so that electrolyte component is constant, i.e. reaction on the general not appreciable impact Electrode of the performance of auxiliary electrode.But the best approach of working electrode interference is probably the solution isolating two electrode districts with sintered glass, porous ceramics or ion exchange membrane etc. by the reaction on minimizing auxiliary electrode.In order to avoid auxiliary electrode produces the impact of any characteristic to measuring the data arrived, the structure of auxiliary electrode still there is certain requirement.As, compared with working electrode, auxiliary electrode should have big surface area and make the polarization added by outside mainly act on working electrode.The resistance of auxiliary electrode own is little, and is not easy polarization, its shape and position is also required simultaneously.
Reference electrode, refers to the electrode not polarized close to ideal of a known electromotive force.Reference electrode is substantially free of electric current pass through, is used for measuring the electrode potential of Electrode (relative to reference electrode).In controlling potential is tested, because reference half-cell keeps fixing electromotive force, thus any changing value of the electromotive force being added on electrochemical cell directly shows on the interface of working electrode/electrolyte solution.It practice, reference electrode plays both provides thermodynamics reference, the dual function again working electrode isolated as research system.Reference electrode common in water solution system has: saturated calomel electrode (SCE), Ag/AgCl electrode, standard hydrogen electrode (SHE or NHE) etc..Many organic electrochemistry measurements carry out in nonaqueous solvent, although aqueous solution reference electrode can also use, but inevitably donor frenulum enters moisture, influence research effect, therefore, it is suggested that be preferably used non-water reference system.Conventional non-water reference system is Ag/Ag+ (acetonitrile).
In the present embodiment, working electrode 4 is identical with auxiliary electrode 2, is diameter platinum disk electrode between 0.1～5 millimeter, shown in Fig. 2, Fig. 3 and Fig. 4, and working electrode 4 and auxiliary electrode 2 distribution at a right angle；Described working electrode 4 is relative with the sample introduction pipeline installing sample introduction draw point 1；Described waste-solution line 5 is relative with auxiliary electrode 2 and not in the same plane.
Embodiment 3:
Sample introduction pipeline is illustrated by the present embodiment on the basis of embodiment 1 and embodiment 2, and it is inner that sample introduction draw point 1 is arranged on sample introduction pipeline, and sample introduction pipeline is plastic tube, and its material is PTFE or other plastics；Described sample introduction draw point 1 length is between 1.0～10 centimetres, and pin hole internal diameter is between 0.2～1.0 millimeter；Described sewer pipe 5 material is PTFE or other plastic tube.
Sample introduction draw point 1 and sample introduction pipeline form the structure of syringe needle and needle tubing, by connecting the change of the pump speed of the syringe pump 7 of sample introduction pipeline, the ejection curve of the sample of ejection in pin hole from sample introduction draw point 1 can be adjusted so that it is can be ejected on working electrode 4, and all be excited on working electrode 4 surface.
Embodiment 4:
The present embodiment is except meeting the feature in above three embodiment, and in the present embodiment, the material of detection cell cell body is quartz, glass or lucite.Sample introduction draw point 1, auxiliary electrode 2, reference electrode 3, working electrode 4 and waste-solution line 5 are arranged on by joint in the passage of detection cell cell body respectively, the outside of joint is with the screw thread matched with detection cell cell body vias inner walls, and the material of joint is lucite or PEEK.
Embodiment 5:
The present embodiment illustrates in conjunction with concrete enforcement data:
In detection cell as shown in Figures 1 to 4, sample introduction draw point 1 is connected with the syringe pump 7 of Electrochemial luminescence detecting instrument by pipeline, working electrode 4 on detection cell, reference electrode 3 and the connection of auxiliary electrode 2 electrode clamp corresponding with in instrument respectively.
Before experiment starts, being full of phosphate buffer (pH8.0,0.1mol/L) in detection cell inside and pipeline, the voltage of working electrode 4 is set to 1.25V.
First with the sample solution of syringe pump just 70 microlitres, (tris (bipyridine) ruthenium, concentration is 1 × 10-6Mol/L) it is pushed in pipeline with the flow velocity of 100 μ l/s, then by the phosphate buffer (pH8.0 of 500 microlitres, 0.1mol/L) it is pushed in pipeline with identical syringe pump with the flow velocity of 50 μ l/s, sample solution is made to enter detection cell, 4 add under the voltage energy effect of 1.25V on the working electrode (s, the trichlorine bipyridyl ruthenium [Ru (bpy) of bivalence3]2+Release electronics generation oxidation reaction and become the trichlorine bipyridyl ruthenium [Ru (bpy) of trivalent3]3+, additionally, the trichlorine bipyridyl ruthenium of bivalence [Ru (bpy)3]2+Also can obtain electronics generation reduction reaction and become the trichlorine bipyridyl ruthenium [Ru (bpy) of monovalence3]+.So, reaction system exists for the trichlorine bipyridyl ruthenium [Ru (bpy) with the trivalent of strong oxidizing property3]3+With [the Ru (bpy) with strong reducing property3]+, react between the two, generate the trichlorine bipyridyl ruthenium [Ru (bpy) of the bivalence of ground state3]2+Trichlorine bipyridyl ruthenium [Ru (bpy) with the bivalence of excited state3]2+, excited state [Ru (bpy)3]2+Decay with Fluorescence Mechanism and release energy to discharge the mode that a wavelength is 62Onm photon, and becoming [the Ru (bpy) of ground state3]2+.The intensity of the light that reaction discharges is detected by the photomultiplier tube in Electrochemial luminescence detecting instrument device, obtains detection spectrogram as shown in Figure 5.
After having detected, the phosphate buffer (pH8.0,0.1mol/L) of 500 microlitres enters detection cell, and detection cell cell body inner chamber is carried out, and waste liquid is flowed out by waste-solution line 5.
As shown in Figure 5, even if the concentration of tris (bipyridine) ruthenium sample is low to moderate 1 × 10-6Mol/L, the signal to noise ratio of signal, still far more than 3, is only capable of to similar static detection method detecting that concentration is 10-4The tris (bipyridine) ruthenium sample of mol/L is compared, and the detection limit of the present embodiment obtains and is obviously improved.
In sum, this electrochemical luminescence detecting pool of the present invention, by syringe pump, sample is sent into pipeline, detection cell under the propelling movement of buffer, and it is injected into the surface of working electrode, by selecting the flow velocity of suitable syringe pump, guarantee that sample is all ejected into the surface of working electrode, present configuration is simple, easy to operate, namely the flow velocity that only need to adjust syringe pump can ensure that whole sample is all excited at working electrode surface, from without the distance artificially determined between draw point end and working electrode.
Exemplified as above is only illustration to the present invention, is not intended that the restriction to protection scope of the present invention, every belongs within protection scope of the present invention with the same or analogous design of the present invention.
Claims (5)
1. an electrochemical luminescence detecting pool, including detection cell cell body, three-electrode system and solution stream, it is characterized in that: in described detection cell cell body, be provided with a vertical channel and four horizontal channels connected with vertical channel, described vertical channel is through-hole structure, via bottoms is provided with a quartz glass plate (6), four described horizontal channels include three passages being in same level and a horizontal channel higher than its excess-three passage, it is respectively mounted working electrode (4) in three described passages being in same level, auxiliary electrode (2) and sample introduction draw point (1), a remaining passage connects waste-solution line (5), reference electrode (3) is installed in described vertical channel；
Described installation sample introduction draw point (1) pipeline is as sample introduction pipeline, and its outer end connects syringe pump (7) by pipeline, and the pump chamber of syringe pump (7) is respectively communicated with sample bottle (8) and buffer bottle (9).
2. electrochemical luminescence detecting pool as claimed in claim 1, it is characterized in that: described working electrode (4) is identical with auxiliary electrode (2), it is diameter platinum disk electrode between 0.1～5 millimeter, working electrode (4) and auxiliary electrode (2) distribution at a right angle；Described working electrode (4) is relative with the sample introduction pipeline installing sample introduction draw point (1)；Described waste-solution line (5) is relative with auxiliary electrode (2) and not in the same plane.
3. electrochemical luminescence detecting pool as claimed in claim 1 or 2, it is characterised in that: it is inner that described sample introduction draw point (1) is arranged on sample introduction pipeline, and sample introduction pipeline is plastic tube；Described sample introduction draw point (1) length is between 1.0～10 centimetres, and pin hole internal diameter is between 0.2～1.0 millimeter；Described waste-solution line (5) is plastic tube.
4. electrochemical luminescence detecting pool as claimed in claim 1, it is characterised in that: the material of described detection cell cell body is quartz, glass or lucite.
5. the electrochemical luminescence detecting pool as described in claim 1 or 4, it is characterized in that: described sample introduction draw point (1), auxiliary electrode (2), reference electrode (3), working electrode (4) and waste-solution line (5) are arranged on by joint in the passage of detection cell cell body respectively, the outside of joint is with the screw thread matched with detection cell cell body vias inner walls, and the material of joint is lucite or PEEK.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610292303.4A CN105806833A (en) | 2016-05-05 | 2016-05-05 | Electrochemical luminescence detection pool |
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| CN201610292303.4A CN105806833A (en) | 2016-05-05 | 2016-05-05 | Electrochemical luminescence detection pool |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109374702A (en) * | 2018-11-22 | 2019-02-22 | 中国科学院金属研究所 | A kind of spray wall type electrochemistry flowing pool device and its application method |
| CN113030068A (en) * | 2021-02-01 | 2021-06-25 | 苏州易莱生物技术有限公司 | Electrochemiluminescence detection device |
| CN114279968A (en) * | 2021-12-28 | 2022-04-05 | 中国科学院长春应用化学研究所 | Electrochemistry-double polarization interference photoelectric detection cell |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109374702A (en) * | 2018-11-22 | 2019-02-22 | 中国科学院金属研究所 | A kind of spray wall type electrochemistry flowing pool device and its application method |
| CN109374702B (en) * | 2018-11-22 | 2024-01-19 | 中国科学院金属研究所 | Wall-spraying type electrochemical flow cell device and use method thereof |
| CN113030068A (en) * | 2021-02-01 | 2021-06-25 | 苏州易莱生物技术有限公司 | Electrochemiluminescence detection device |
| CN114279968A (en) * | 2021-12-28 | 2022-04-05 | 中国科学院长春应用化学研究所 | Electrochemistry-double polarization interference photoelectric detection cell |
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