CN108896775A - A kind of Portable egg white matter quantitative testing device and method - Google Patents
A kind of Portable egg white matter quantitative testing device and method Download PDFInfo
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- CN108896775A CN108896775A CN201810449153.2A CN201810449153A CN108896775A CN 108896775 A CN108896775 A CN 108896775A CN 201810449153 A CN201810449153 A CN 201810449153A CN 108896775 A CN108896775 A CN 108896775A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
Abstract
The invention discloses a kind of Portable egg white matter quantitative testing device and methods, device includes micro-fluidic detection chip, miniature spectrophotometric detector part, control module, power module and communication module, micro-fluidic detection chip includes incident light generating apparatus, reaction tank and mini optical fibre, mini optical fibre connects miniature spectrophotometric detector part on the outside of micro-fluidic detection chip, incident light generating apparatus, miniature spectrophotometric detector part, it is electrically connected between power module and communication module, method utilizes Portable egg white matter quantitative testing device, assist matched reagent, it just can return to quantification of protein information by simple operations process.The advantages of the present invention over the prior art are that：Provide a kind of portable detector and method for biochemical reaction analysis, it is tested and analyzed for quantification of protein, since its is compact-sized, small volume can integrate very well adapts to one column detection and analysis of micro-current control biochemical analysis chip completion, provides possibility for miniaturization field screening analytical technology.
Description
Technical field
The present invention relates to protein monitoring professional domain, in particular to a kind of Portable egg white matter quantitative testing device and sides
Method.
Background technique
Protein is the important indicator of food nutrition and the important biomolecule macromolecular of biochemical analysis, and many biochemical indicators are very
Detecting final test object to specific immunity is all protein, and it is larger that current commercially instrument both domestic and external all suffers from volume, at
This height, the high problem of price, therefore cause to be difficult to realize portability biochemistry biochemical analysis, in particular for food safety and face
Screen grading detection is looked at the scene of bed medical treatment.
Summary of the invention
The technical problem to be solved by the present invention is to overcome the above technological deficiencies, provide a kind of suitable miniaturization field screening point
The Portable egg white matter quantitative testing device and method of analysis.
In order to solve the above technical problems, technical solution provided by the invention is：A kind of Portable egg white matter quantitative detection dress
It sets, including micro-fluidic detection chip, miniature spectrophotometric detector part, control module, power module and communication module, the miniflow
Controlling detection chip includes incident light generating apparatus, reaction tank and mini optical fibre, the incident light generating apparatus, reaction tank and micro-
Type optical fiber is successively arranged adjacent in the same horizontal position, and the mini optical fibre connects miniature point on the outside of micro-fluidic detection chip
Light detecting device is electrically connected between the incident light generating apparatus, miniature spectrophotometric detector part, power module and communication module
It connects.
The advantages of the present invention over the prior art are that：Provide a kind of portable inspectiont for biochemical reaction analysis
Apparatus and method, for quantification of protein test and analyze, since its is compact-sized, small volume can integrate very well adapt to it is micro-
Flow control biochemical analysis chip is completed a column and is tested and analyzed, and provides possibility for miniaturization field screening analytical technology.
As an improvement, the micro-fluidic detection chip includes reagent A liquid storage tank, injection port, waste liquid pool and reagent B liquid storage
Pond, it is arranged in a straight line between the injection port, reaction tank and waste liquid pool, the injection port two sides be equipped with reagent A liquid storage tank and
Reagent B liquid storage tank, the reagent A liquid storage tank connect reaction tank, the micro-fluidic detection core with reagent B liquid storage tank branch pipe
Piece substrate uses organic glass material.
As an improvement, the incident light generating apparatus uses small-sized white light LEDs light bulb, used in the communication module
Wireless communications chips, the power module use lithium ion battery.
As an improvement, the micro-fluidic detection chip is encapsulated using PDMS flexible material, the control module, power supply
It is integral that module and communication module integrally use integrative packaging technology to form a collection.
In order to solve the above technical problems, the present invention also provides the methods using above-mentioned detection device detection protein：One
Quantification of protein detection method of the kind based on a kind of Portable egg white matter quantitative testing device as described in claim 1, packet
Include following steps：
(a) Portable egg white matter quantitative testing device is subjected to normal concentration calibration, and by controller module generation
Portion's calibrating patterns；
(b) testing protein solution is stand-by as tested protein example by pretreatment processing；
(c) by the injection port of tested protein example injection micro-fluidic detection chip, reaction tank is introduced the sample into, is squeezed micro-
PDMS flexible package layer of the flow control detection chip above reagent A liquid storage tank, makes the reagent A in reagent A liquid storage tank pass through straight tube
It is sufficiently mixed with tested protein example in reaction tank, constant temperature stands 10min.
(d) PDMS flexible package layer of the micro-fluidic detection chip above reagent B liquid storage tank is squeezed, reagent B liquid storage tank is made
Interior reagent B is sufficiently mixed with tested protein example, reagent A in reaction tank by branch pipe, and constant temperature stands 30min.
(e) it is communicated wirelessly by the intelligent mobile terminal being adapted to by communication module and control module, open detection
Mode, control module control switch and the working time of incident light generating apparatus, and receive miniature spectrophotometric detector part simultaneously
Detect signal, through control module operation, and compared with internal calibration model after calculate quantification of protein result.
As an improvement, the reagent A and reagent B are the standard protein analytical reagents with chromogenic reaction, convenient for and
Tested protein example reaction, goes on smoothly experimental implementation.
Detailed description of the invention
Fig. 1 is a kind of overall structure diagram of Portable egg white matter quantitative testing device and method of the present invention.
Fig. 2 is the micro-fluidic detection chip structure signal of a kind of Portable egg white matter quantitative testing device of the present invention and method
Figure.
Fig. 3 is a kind of reaction tank connection schematic diagram of Portable egg white matter quantitative testing device and method of the present invention.
Fig. 4 is a kind of BSA calibration curve schematic diagram of Portable egg white matter quantitative testing device and method of the present invention.
As shown in the figure：1, micro-fluidic detection chip；2, miniature spectrophotometric detector part；3, control module；4, power module；5,
Communication module；11, incident light generating apparatus；12, reaction tank；13, mini optical fibre；14, reagent A liquid storage tank；15, injection port；16,
Waste liquid pool；17, reagent B liquid storage tank.
Specific embodiment
Present invention will be described in further detail below with reference to the accompanying drawings.
In conjunction with Fig. 1, a kind of Portable egg white matter quantitative testing device, including micro-fluidic detection chip 1, miniature light splitting detection
Device 2, control module 3, power module 4 and communication module 5, the micro-fluidic detection chip 1 include incident light generating apparatus
11, reaction tank 12 and mini optical fibre 13, the incident light generating apparatus 11, reaction tank 12 and mini optical fibre 13 successively adjacent row
It is listed in the same horizontal position, the mini optical fibre 13 connects miniature spectrophotometric detector part 2 on the outside of micro-fluidic detection chip 1,
The miniature light splitting for the model C 10988MA-01 that the miniature spectrophotometric detector part 2 is produced using HAMAMATSU (shore pine) company
Device is detected, it is electric between the incident light generating apparatus 11, miniature spectrophotometric detector part 2, power module 4 and communication module 5
Connection, wherein control module 3, power module 4 and 5 entirety of communication module are integrated whole using integrative packaging technology composition one
Body, save device taken up space, control module 3 by with incident light generating apparatus 11, miniature spectrophotometric detector part 2, communicate mould
Electrical connection between block 5, controls the switch and runing time of incident light generating apparatus 11, and control module 3 receives miniature light splitting inspection
It surveys the detection data of device 2 and carries out storage analysis, and final detection result is transmitted to by host computer or shifting by communication module 5
Dynamic terminal.
In conjunction with Fig. 2 and Fig. 3, the micro-fluidic detection chip 1 includes reagent A liquid storage tank 14, injection port 15, waste liquid pool 16
It is arranged in a straight line between the injection port 15, reaction tank 12 and waste liquid pool 16 to constitute protein example with reagent B liquid storage tank 17
Detection ordering channel, 15 two sides of injection port are equipped with reagent A liquid storage tank 14 and reagent B liquid storage tank 17, the reagent A storage
Liquid pool 14 connects reaction tank 12 with 17 branch pipe of reagent B liquid storage tank, and the micro-fluidic detection chip 1 uses PDMS flexible material
Encapsulation can be reacted by squeezing reagent reservoir to introduce the reagents into reaction tank.
Specific experimental method is as follows：
(A) the BSA albumen of normal concentration gradient is calibrated first with the device of the invention and method
Experiment reagent：Reagent A：Stable type alkalinity copper reagent 10mL；
Calibrate solution：BSA standard protein original liquid concentration：1mg/mL2mL；
Reagent B：Folin- phenol reagent 1N1mL；
Experimental procedure：
(a) reagent prepares
The BSA standard solution of according to the form below preparation various concentration gradient.
(b) Concentration Testing
1, the minute sample pond for preparing 7 adaptation detection devices of the present invention, is arranged sample label 1~7, removes No. 1 air sample
This is outer, and the standard solution that table on 200 μ L prepares BSA concentration gradient is added by sample number.
2, prepare 7 standard microfluidic detection chips and be separately added into 1~No. 7 sample,
3 and PDMS flexible package layer of the micro-fluidic detection chip 1 above reagent A liquid storage tank 14 is squeezed respectively, make to try
Agent A is sufficiently mixed with tested protein example in reaction tank 12, and constant temperature stands 10min.
4, PDMS flexible package layer of the micro-fluidic detection chip 1 above reagent B liquid storage tank 17 is squeezed respectively, makes reagent B
Constant temperature, which is sufficiently mixed, in reaction tank 12 with tested protein example, reagent A stands 30min.
5, take label 1-7 micro-fluidic detection chip as calibration curve.
6,1-7 micro-fluidic chip is sequentially inserted into device, is carried out by the intelligent mobile terminal and control module 3 of adaptation
Calibration mode is opened in wireless telecommunications, and control module 3 controls unlatching and the working time of incident light generating apparatus 11, and connects simultaneously
The detection signal (luminous intensity) of miniature spectrophotometric detector part 2 is received, control module 3 inhales 2-7 sample and No. 1 air sample
Luminosity conversion, and calibrated according to the actual concentrations of the standard protein inputted, form concentration homologous thread and formula.
It is as follows using apparatus of the present invention and method measurement correction group result：
Device internal controller calibration curve such as Fig. 4：
Dependent equation：Y=213.88X-29.184 coefficient R2=0.99
(B) according to above-mentioned resulting BSA calibration curve, quantification of protein detection is carried out using apparatus of the present invention
(a) using apparatus of the present invention after calibration, prepare the minute sample pond of 1 adaptation detection device of the present invention, be put into
Normal concentration is the protein example of 100 μ g/mL, and micro-fluidic detection chip 1 is added as sample.
(b) it by the injection port of sample injection micro-fluidic detection chip 1, and squeezes micro-fluidic detection chip 1 and is trying
The PDMS flexible package layer of 14 top of agent A liquid storage tank, is sufficiently mixed reagent A in reaction tank 12 with tested protein example, room
Temperature stands 10min.
(c) PDMS flexible package layer of the micro-fluidic detection chip 1 above reagent B liquid storage tank 17 is squeezed, reagent B and quilt are made
It surveys protein example to be sufficiently mixed in reaction tank 12, is stored at room temperature 30min.
(d) it is communicated wirelessly by the intelligent mobile terminal of adaptation with control module 3, open detection mode, controls mould
Block 3 controls unlatching and the working time of incident light generating apparatus 11, and receives the detection signal of miniature spectrophotometric detector part 2 simultaneously
(luminous intensity), connecting inner calibrating patterns, extrapolates testing result.
(e) being tested group absorbance is 0.6040, and corresponding calibration curve, it is 100 μ g/ that detection device of the present invention, which returns to concentration,
ML, it is consistent with sample actual concentrations.
The present invention and its embodiments have been described above, this description is no restricted, shown in the drawings
Only one of embodiments of the present invention, actual structure is not limited to this.All in all if the ordinary skill of this field
Personnel are enlightened by it, without departing from the spirit of the invention, are not inventively designed and the technical solution phase
As frame mode and embodiment, be within the scope of protection of the invention.
Claims (6)
1. a kind of Portable egg white matter quantitative testing device, it is characterised in that：Including micro-fluidic detection chip (1), miniature light splitting
Detection device (2), control module (3), power module (4) and communication module (5), the micro-fluidic detection chip (1) include
Incident light generating apparatus (11), reaction tank (12) and mini optical fibre (13), the incident light generating apparatus (11), reaction tank
(12) and mini optical fibre (13) is successively arranged adjacent in the same horizontal position, and the mini optical fibre (13) is in micro-fluidic detection core
Miniature spectrophotometric detector part (2), the incident light generating apparatus (11), miniature spectrophotometric detector part are connected on the outside of piece (1)
(2), it is electrically connected between power module (4) and communication module (5).
2. a kind of Portable egg white matter quantitative testing device according to claim 1 and method, it is characterised in that：Described
Micro-fluidic detection chip (1) includes reagent A liquid storage tank (14), injection port (15), waste liquid pool (16) and reagent B liquid storage tank (17),
Arranged in a straight line between the injection port (15), reaction tank (12) and waste liquid pool (16), described injection port (15) two sides are equipped with
Reagent A liquid storage tank (14) and reagent B liquid storage tank (17), the reagent A liquid storage tank (14) and reagent B liquid storage tank (17) branch pipe connect
It connects reaction tank (12), micro-fluidic detection chip (1) substrate uses organic glass material.
3. a kind of Portable egg white matter quantitative testing device according to claim 1 and method, it is characterised in that：Described
Incident light generating apparatus (11) uses small-sized white light LEDs light bulb, uses wireless communications chips, institute in the communication module (5)
The power module (4) stated uses lithium ion battery.
4. a kind of Portable egg white matter quantitative testing device according to claim 1 and method, it is characterised in that：Described
Micro-fluidic detection chip (1) is encapsulated using PDMS flexible material, the control module (3), power module (4) and communication module
(5) whole integral using integrative packaging technology one collection of composition.
5. a kind of quantification of protein detection based on a kind of Portable egg white matter quantitative testing device as described in claim 1
Method, it is characterised in that：Include the following steps：
(a) Portable egg white matter quantitative testing device is subjected to normal concentration calibration, and generates inside by controller module (3)
Calibrating patterns；
(b) testing protein solution is stand-by as tested protein example by pretreatment processing；
(c) by the injection port (15) of tested protein example injection micro-fluidic detection chip (1), reaction tank is introduced the sample into
(12), the PDMS flexible package layer of micro-fluidic detection chip (1) above reagent A liquid storage tank (14) is squeezed, reagent A liquid storage tank is made
(14) reagent A in is sufficiently mixed with tested protein example in reaction tank (12) by straight tube, and constant temperature stands 10min.
(d) the PDMS flexible package layer of micro-fluidic detection chip (1) above reagent B liquid storage tank (17) is squeezed, reagent B liquid storage is made
Reagent B in pond (17) is sufficiently mixed with tested protein example, reagent A in reaction tank by branch pipe, and constant temperature stands 30min.
(e) it is communicated wirelessly by communication module (5) with control module (3) by the intelligent mobile terminal of adaptation, opens inspection
Survey mode, control module (3) control switch and the working time of incident light generating apparatus (11), and receive miniature light splitting inspection simultaneously
The detection signal for surveying device (2), through control module (3) operation, and compared with internal calibration model after to calculate protein fixed
Measure result.
6. quantification of protein detection method as claimed in claim 5, it is characterised in that：The reagent A is to have with reagent B
The standard protein analytical reagent of chromogenic reaction.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810449153.2A CN108896775A (en) | 2018-05-11 | 2018-05-11 | A kind of Portable egg white matter quantitative testing device and method |
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| CN201810449153.2A CN108896775A (en) | 2018-05-11 | 2018-05-11 | A kind of Portable egg white matter quantitative testing device and method |
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Cited By (2)
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| WO2020259253A1 (en) * | 2019-06-26 | 2020-12-30 | 京东方科技集团股份有限公司 | Analysis device for detection chip, and operation method and analysis system thereof |
| CN113049478A (en) * | 2021-04-25 | 2021-06-29 | 中国计量科学研究院 | Protein aggregate analysis and detection device based on micro-fluidic chip and working method |
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| CN113049478A (en) * | 2021-04-25 | 2021-06-29 | 中国计量科学研究院 | Protein aggregate analysis and detection device based on micro-fluidic chip and working method |
| CN113049478B (en) * | 2021-04-25 | 2022-05-17 | 中国计量科学研究院 | Protein aggregate analysis and detection device based on micro-fluidic chip and working method |
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