CN211013774U

CN211013774U - Protein electrophoresis boils appearance device

Info

Publication number: CN211013774U
Application number: CN201922069562.2U
Authority: CN
Inventors: 晁迪; 董文佩; 李峰; 许嘉琪
Original assignee: Henan Normal University
Current assignee: Henan Normal University
Priority date: 2019-11-26
Filing date: 2019-11-26
Publication date: 2020-07-14
Anticipated expiration: 2029-11-26

Abstract

The utility model discloses a kind device is boiled in albumen electrophoresis, including albumen sample splendid attire pipe and mount, the mount includes roof and a bottom plate that the level set up, roof and bottom plate are through four support column fixed connection, be provided with a plurality of albumen sample splendid attire pipe jack on the roof, all be provided with the outstanding interior spiral recess of outer spiral with the corresponding position department of albumen sample splendid attire pipe jack that sets up on the roof on the bottom plate, the bottom of albumen sample splendid attire pipe be provided with the bottom plate on the outstanding interior spiral recess assorted of the outstanding interior spiral of outer spiral of setting. Utilize the device can make the vertical fixing of albumen sample splendid attire pipe boil the appearance in the water bath, can avoid albumen sample splendid attire pipe to receive the buoyancy influence of water bath and remove and cause the sample to obscure, make things convenient for taking of albumen sample, can also avoid albumen sample to leak or the water bath gets into the albumen sample splendid attire pipe in pollutes the albumen sample.

Description

Protein electrophoresis boils appearance device

Technical Field

The utility model belongs to the technical field of biochemical experimental apparatus, concretely relates to albumen electrophoresis boils appearance device.

Background

Protein electrophoresis is a conventional technology in the field of biotechnology, and is a technology for separating different proteins by utilizing the difference of charges in a buffer solution with the same pH value due to the difference of isoelectric points and molecular sizes of the proteins and different electrophoretic mobility rates and directions in an electric field, so that different protein molecules have different electrophoretic mobility rates. Common protein electrophoresis techniques mainly include polyacrylamide gel electrophoresis and SDS-polyacrylamide gel electrophoresis. In the SDS-polyacrylamide gel electrophoresis technology, the sample is generally boiled to destroy the secondary tertiary structure of protein and open disulfide bond, so that the protein becomes linear and is combined with a large amount of SDS, the negative charge of SDS is used for shielding the charge of protein, thus the speed of protein electrophoresis is only related to the size of molecular weight, and the influence of factors such as protein structure and conformation on the electrophoretic migration of protein is avoided. At present, the boiling treatment of protein samples is mainly to utilize floating to boil the water bath, namely, a floating centrifugal tube containing the protein samples is directly put into a water bath kettle to be heated in the water bath kettle, and the floating centrifugal tube containing the protein samples can move randomly in the water bath kettle under the buoyancy action of water, so that the samples are mixed up, and the samples are not convenient to take. On the other hand, the floating centrifuge tube filled with the protein sample directly floats for water bath, and when the cover of the floating centrifuge tube is not tightly covered, the sample is easily leaked, or the water bath enters the sample to pollute the sample.

Therefore, it is necessary to develop a protein electrophoresis boiling device capable of fixing a plurality of protein samples in a water bath for boiling at the same time to solve the defects of floating boiling.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a can fix a plurality of albumen samples simultaneously and boil the albumen electrophoresis appearance device that boils in the water bath.

In order to realize the above object, the technical scheme of the utility model is that:

the utility model provides a albumen electrophoresis boils appearance device, includes albumen sample splendid attire pipe and the mount that is used for fixed albumen sample splendid attire pipe, the mount includes a roof and a bottom plate that the level set up, roof and bottom plate are through four support column fixed connection, be provided with a plurality of albumen sample splendid attire pipe jack on the roof, all be provided with the protruding interior spiral recess of outer spiral with the corresponding position department of albumen sample splendid attire pipe jack that sets up on the roof on the bottom plate, the bottom of albumen sample splendid attire pipe be provided with the bottom plate on the protruding interior spiral recess assorted of outer spiral/outer spiral protruding.

When a protein sample is boiled, the fixing frame is placed in a water bath, then the protein sample containing pipe containing the protein sample is inserted into the fixing frame, and then the protein sample containing pipe is rotated to enable the outer spiral protrusion/inner spiral groove at the bottom of the protein sample containing pipe to be screwed with the inner spiral groove/outer spiral protrusion arranged on the bottom plate of the fixing frame, so that the protein sample containing pipe containing the protein sample can be vertically fixed on the fixing frame.

Furthermore, the protein sample containing pipe jacks are uniformly distributed on the top plate.

Further, the top plate and the bottom plate are both circular or rectangular.

Furthermore, the protein sample containing pipe is also provided with a protein sample containing pipe sealing cover.

Furthermore, the protein electrophoresis sample boiling device is also provided with a top plate sealing cover, the shape and the size of the top plate sealing cover are equivalent to those of the top plate, and when the top plate sealing cover is covered with the top plate, all the protein sample containing pipes on the sealing fixing frame can be covered and sealed simultaneously. When a plurality of protein samples need to be moved simultaneously, the sealing cover of the top plate is only required to be covered on the top plate, so that the sealing of all protein sample containing pipes can be realized, and each protein sample containing pipe does not need to be separately sealed, so that the protein sample can be prevented from vibrating and overflowing in the moving process, the operation is fast and simple, and the experimental efficiency can be effectively improved.

Further, a rubber pad is arranged in the top plate sealing cover, and a groove matched with the pipe orifice of the protein sample containing pipe is formed in the rubber pad.

Furthermore, the top plate sealing cover and the top plate are in press fit sealing in a buckling mode.

The utility model has the advantages that: utilize the albumen electrophoresis boil appearance device can make the vertical fixing of albumen sample splendid attire pipe that is equipped with the albumen sample boil the appearance in the water bath, can avoid albumen sample splendid attire pipe to receive the buoyancy influence of water bath and remove and cause the sample to obscure on the one hand, make things convenient for taking of albumen sample, on the other hand can also avoid the albumen sample to leak or the water bath to get into the albumen sample splendid attire pipe in pollutes the albumen sample.

Drawings

FIG. 1 is a schematic structural diagram of a protein electrophoresis sample boiling device according to an embodiment of the present invention;

FIG. 2 is a schematic structural view of a sample holding tube according to an embodiment of the present invention;

fig. 3 is a schematic structural view of a sealing cover according to an embodiment of the present invention;

FIG. 4 is a schematic view of the protein electrophoresis sample boiling device covered with the sealing cover according to the embodiment of the present invention;

description of the figure numbering: 1. the bottom plate, 2, the support column, 3, the roof, 4, the outer spiral is outstanding, 5, albumen sample splendid attire pipe jack, 6, albumen sample splendid attire pipe, 61, albumen sample splendid attire pipe body, 62, interior spiral recess, 63, albumen sample splendid attire pipe sealed lid, 7, roof sealed lid, 8, rubber pad, 81, recess, 9, buckle.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. All other embodiments obtained by a person skilled in the art based on the embodiments of the present invention belong to the protection scope of the present invention.

Example 1

As shown in fig. 1 and 2, a kind of device is boiled in albumen electrophoresis, including albumen sample splendid attire pipe 6 and the mount that is used for fixed albumen sample splendid attire pipe 6, the mount includes roof 3 and bottom plate 1 that the level set up, roof 3 and bottom plate 1 are through four support column 2 fixed connection, be provided with a plurality of albumen sample splendid attire pipe jack 5 on the roof 3, all be provided with outer spiral salient/interior spiral recess with the position department that albumen sample splendid attire pipe jack 5 that sets up on the roof 3 on the bottom plate 1, the bottom of albumen sample splendid attire pipe 6 be provided with the bottom plate 1 on set up outer spiral salient/interior spiral recess assorted interior spiral recess/outer spiral salient.

The protein sample holding tube insertion holes 5 can be arranged on the top plate 3 in an evenly distributed manner, can also be arranged on the top plate 3 in a staggered distribution manner, and are preferably arranged on the top plate 3 in an evenly distributed manner.

The top plate 3 and the bottom plate 1 may be of any possible shape, preferably circular or rectangular.

The protein sample containing pipe 6 can be provided with a protein sample containing pipe sealing cover 63, or can not be provided with the protein sample containing pipe sealing cover 63, so that the protein sample containing pipe sealing cover 63 is preferably arranged to avoid oscillation and overflow when the sample is moved. The sealing cover 63 of the protein sample containing tube can be a buckle type sealing cover or a rotary type sealing cover, preferably an outward rotary type sealing cover, on one hand, the outward rotary type sealing cover is simple and quick to operate when sealed, the sealing effect is good, and on the other reverse side, the sample can be prevented from being polluted by foreign matters stained on the sealing cover.

In this embodiment, only the bottom plate 1 is provided with the outer spiral protrusions 4, the bottom of the protein sample holding tube 6 is provided with the inner spiral grooves 62, and the shapes of the top plate 3 and the bottom plate 1 are illustrated as rectangular shapes.

Example 2

As shown in fig. 3 and 4, in order to further improve the experimental efficiency, the protein electrophoresis sample boiling device is further provided with a top plate sealing cover 7, the shape and size of the top plate sealing cover 7 are both equivalent to those of the top plate 3, a rubber pad 8 is arranged in the top plate sealing cover 7, a groove 81 matched with the pipe orifice of the protein sample containing pipe 6 is arranged on the rubber pad 8, and the top plate sealing cover 7 and the top plate 3 are pressed and sealed through a buckle 9.

In this embodiment, only the top plate 3 and the bottom plate 1 are illustrated as being rectangular, and in this embodiment, there are 4 fasteners 9 respectively disposed at the middle positions of the four sides of the rectangular top plate 3.

When a plurality of protein samples need to be moved simultaneously, only the sealing cover 7 of the top plate needs to be covered on the top plate 3, and then 4 buckles 9 arranged in the middle positions of the four sides of the top plate 3 are fastened, so that the pressing and sealing of all the protein sample containing pipes 6 can be realized, and the operation is fast and simple.

The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not intended to limit the present invention, and all modifications, equivalents, improvements and the like that are within the spirit and principle of the present invention should be included in the scope of the present invention.

Claims

1. The utility model provides a albumen electrophoresis boils appearance device, its characterized in that, includes albumen sample splendid attire pipe and the mount that is used for fixed albumen sample splendid attire pipe, the mount includes a roof and a bottom plate that the level set up, roof and bottom plate are through four support column fixed connection, be provided with a plurality of albumen sample splendid attire pipe jack on the roof, all be provided with outer spiral salient/interior spiral recess with the corresponding position department of albumen sample splendid attire pipe jack that sets up on the roof on the bottom plate, the bottom of albumen sample splendid attire pipe be provided with the bottom plate on set up outer spiral salient/interior spiral recess assorted interior spiral recess/outer spiral salient.

2. The protein electrophoresis sample boiling device of claim 1, wherein the protein sample containing tube jacks are uniformly distributed on the top plate.

3. The protein electrophoresis sample boiling device of claim 1, wherein the top plate and the bottom plate are both circular or rectangular.

4. The protein electrophoresis sample boiling device of claim 1, wherein the protein sample containing tube is further provided with a protein sample containing tube sealing cover.

5. The protein electrophoresis sample boiling device as claimed in claim 1, further comprising a top plate sealing cover, wherein the shape and size of the top plate sealing cover are equal to those of the top plate, and when the top plate sealing cover is covered with the top plate, all the protein sample containing tubes on the fixing frame can be simultaneously covered and sealed.

6. The protein electrophoresis sample boiling device as claimed in claim 5, wherein a rubber pad is arranged in the top plate sealing cover, and a groove matched with the pipe orifice of the protein sample containing pipe is arranged on the rubber pad.

7. The protein electrophoresis sample boiling device of claim 5, wherein the top plate sealing cover and the top plate are in press fit sealing through a snap fit manner.