CN203101147U

CN203101147U - Flat-bed electrophoresis gel dyeing and decolorizing tank

Info

Publication number: CN203101147U
Application number: CN 201320002539
Authority: CN
Inventors: 唐超
Original assignee: Qingdao Agricultural University
Current assignee: Qingdao Agricultural University
Priority date: 2013-01-05
Filing date: 2013-01-05
Publication date: 2013-07-31
Anticipated expiration: 2023-01-05

Abstract

The utility model relates to an experimental tool and particularly relates to a flat-bed electrophoresis gel dyeing and decolorizing tank. The flat-bed electrophoresis gel dyeing and decolorizing tank comprises an outer tank, an inner tank, baffles, two outer handle frames, a handle and two screws, wherein the outer tank is a rectangle provided with a hollow cavity, and only the top face of the outer tank is open; the inner tank is a rectangle provided with a hollow cavity, a plurality of mutually parallel rectangular jacks are formed on the left and right end faces of the inner tank, and the inner tank is arranged in the outer tank; the baffles are detachably inserted in the rectangular jacks, the two outer handle frames are respectively locked on the left and right end faces of the inner tank at the left and right sides, and threaded holes are formed at the middles of the top parts of the outer handle frames; two through holes are formed at the two ends close to the handle; and the two screws are inserted in the through holes and the threaded holes for connecting the handle with the two outer handle frames. The flat-bed electrophoresis gel dyeing and decolorizing tank is capable of saving reagents and saving time, unlikely to damage the gel, convenient to operate, suitable for marking and wide in adaptation range, and certain beneficial effects are obtained.

Description

The gel-colored decolouring groove of a kind of disk electrophoresis

Technical field

The utility model relates to a kind of experiment apparatus, the gel-colored decolouring groove of especially a kind of disk electrophoresis.

Background technology

Electrophoretic techniques is the experimental technique of field widespread uses such as biological chemistry, molecular biology, various ways is arranged: vertical slab electrophoresis, disc electrophoresis, horizontal strip electrophoresis, Capillary Electrophoresis etc., vertical slab electrophoresis commonly used and disc electrophoresis in scientific research and testing, majority as separating medium, is suitable for multiple bioactivators such as isolated protein, amino acid, nucleic acid with polyacrylamide gel.The electrophoresis operating process is complicated, length consuming time, and wherein dyeing and decolorization are the final key steps that can test successful.According to the difference of test specimen character, dyeing is different with the decolouring agents useful for same, but dyeing, decolorization is basic identical: the gel that will have different samples separately; Immerse dyeing liquor respectively; Dyeing finishes and takes out the distilled water cleaning with instrument; Immerse destainer respectively; It is complete to colour developing repeatedly to change destainer.

The dyeing and the decolouring groove industrial products that are applicable to that disk electrophoresis polylith gel is operated are not simultaneously arranged at present as yet, and the normal dyeing of each scientific research and testing chamber, decolouring are to adopt double dish or the pallet that is fit to size, general double dish dyeing, decolouring one clotting glue; The dyeing back is blocked gel with instrument and is poured out dye liquor, changes destainer; Repeatedly change destainer up to reaching promising result.

The shortcoming of prior art is: each gel of disk electrophoresis need be wasted reagent with the separately dyeing of different containers, especially does isozyme electrophoresis, and substrate costs an arm and a leg, and can cause unnecessary waste; When doing isoelectric focusing electrophoresis, need to distinguish the two poles of the earth up and down of gel, be difficult to separately when common dyeing and decolouring; The medium majority of electrophoresis is a polyacrylamide gel at present, and this gel is soft flexible, but needs constantly to change liquid in dyeing and decolorization, is easy to damage colloid with the instrument non-productive operation; For the wayward dyeing time of responsive colouring method (dying) as silver.

The utility model content

The utility model is intended to address the above problem, and provides a kind of disk electrophoresis gel-colored decolouring groove, its saves reagent, not fragile gel, saves time, easy to operate, be suitable for mark, applied widely, its technical scheme that adopts is as follows:

The gel-colored decolouring groove of a kind of disk electrophoresis comprises:

Water jacket, it is one to have the cavity and the rectangular parallelepiped of end face opening only;

Inside groove, it is one to have the rectangular parallelepiped that has some the rectangle jacks that are parallel to each other on hollow cavity and the left and right sides both ends of the surface, described inside groove is located in the water jacket;

Baffle plate, it can shirk and be threaded onto in the rectangle jack.

On the technique scheme basis, the gel-colored decolouring groove of this disk electrophoresis also comprises:

Two handle housings split about it on left and right sides end face that is buckled in inside groove, and the crown center place of described handle housing is provided with threaded hole;

Handle is offering two through holes near its place, two ends;

Two screws, it is threaded onto in through hole and the threaded hole, and handle is connected with two handle housings.

On the technique scheme basis, described water jacket and inside groove are square.

On the technique scheme basis, offer several first circular holes on the described inside groove front/rear end, all offer several second circular holes on the right side of the left side of left part handle housing and right part handle housing.

On the technique scheme basis, perk forms stopper protrusion on the side direction of described baffle plate.

The utlity model has following advantage: have multiple action, be applicable to the dyeing and the decolouring of multiple media plate running gel; Polylith gel simultaneously can dye, decolour; Can be used for dyeing and can finish decolorization simultaneously again, perhaps be equipped with a plurality of water jackets, adorn dyeing liquor and destainer respectively, realize instantaneous end dyeing, effectively control the dyeing time of responsive method; Save reagent, not fragile gel, save time, easy to operate, be suitable for mark, applied widely.

Description of drawings

Fig. 1: blast structural representation of the present utility model;

Fig. 2: the plan structure synoptic diagram of water jacket shown in Fig. 1;

Fig. 3: right part handle housing partly cuts open structural representation among Fig. 1;

Fig. 4: the right TV structure synoptic diagram of inside groove shown in Fig. 1;

Fig. 5: the plan structure synoptic diagram of inside groove shown in Fig. 1;

Fig. 6: the plan structure synoptic diagram of baffle plate shown in Fig. 1;

Fig. 7: inside groove shown in Fig. 1 partly cut open structural representation;

Fig. 8: the plan structure synoptic diagram of handle shown in Fig. 1;

Fig. 9: the left TV structure synoptic diagram of the handle of right part shown in Fig. 1 housing;

Symbol description:

1. water jacket, 2. inside groove, 3. baffle plate, 4. handle housing, 5. handle, 6. screw, 10. rectangle jack, 11. threaded holes, 12. stopper protrusion, 13. first circular holes, 14. second circular holes.

Embodiment

The utility model is described in further detail below in conjunction with accompanying drawing and example:

To shown in Figure 9, the gel-colored decolouring groove of a kind of disk electrophoresis comprises as Fig. 1:

Water jacket 1, it is one to have the cavity and the rectangular parallelepiped of end face opening only;

Inside groove 2, it is one to have the rectangular parallelepiped that has some the rectangle jacks 10 that are parallel to each other on hollow cavity and the left and right sides both ends of the surface, described inside groove 2 is located in the water jacket 1;

Baffle plate 3, it can shirk and be threaded onto in the rectangle jack 10.

Preferably, also comprise:

Two handle housings 4 split about it on left and right sides end face that is buckled in inside groove 2, and the crown center place of described handle housing 4 is provided with threaded hole 11;

Handle 5 is offering two through holes near its place, two ends;

Two screws 6, it is threaded onto in through hole and the threaded hole 11, and handle 5 is connected with two handle housings 4.

Preferably, described water jacket 1 and inside groove 2 are square, and square is special rectangular parallelepiped.

Further, offer several first circular holes 13 on described inside groove 2 front/rear ends, all offer several second circular holes 14 on the right side of the left side of left part handle housing 4 and right part handle housing 4, described first circular hole 13 and second circular hole 14 mainly work the solution of coming in and going out.

Further again, perk forms stopper protrusion 12 on the side direction of described baffle plate 3.

With way of example the utility model is illustrated above, but the utility model is not limited to above-mentioned specific embodiment, all any change or modification of doing based on the utility model all belong to the claimed scope of the utility model.

Claims

1. the gel-colored decolouring groove of disk electrophoresis is characterized in that, comprising:

Water jacket (1), it is one to have the cavity and the rectangular parallelepiped of end face opening only;

Inside groove (2), it is one to have the rectangular parallelepiped that has some the rectangle jacks (10) that are parallel to each other on hollow cavity and the left and right sides both ends of the surface, described inside groove (2) is located in the water jacket (1);

Baffle plate (3), it can shirk and be threaded onto in the rectangle jack (10).

2. the gel-colored decolouring groove of a kind of disk electrophoresis according to claim 1 is characterized in that, also comprises:

Two handle housings (4) split about it on left and right sides end face that is buckled in inside groove (2), and the crown center place of described handle housing (4) is provided with threaded hole (11);

Handle (5) is offering two through holes near its place, two ends;

Two screws (6), it is threaded onto in through hole and the threaded hole (11), and handle (5) is connected with two handle housings (4).

3. the gel-colored decolouring groove of a kind of disk electrophoresis according to claim 1 and 2 is characterized in that: described water jacket (1) and inside groove (2) are square.

4. the gel-colored decolouring groove of a kind of disk electrophoresis according to claim 2, it is characterized in that: offer several first circular holes (13) on described inside groove (2) front/rear end, all offer several second circular holes (14) on the right side of the left side of left part handle housing (4) and right part handle housing (4).

5. the gel-colored decolouring groove of a kind of disk electrophoresis according to claim 1 is characterized in that: perk forms stopper protrusion (12) on the side direction of described baffle plate (3).