CN201058857Y

CN201058857Y - Bi-functional electrophoresis tank

Info

Publication number: CN201058857Y
Application number: CNU2007200317746U
Authority: CN
Inventors: 刘利兵; 陈志阳; 杨芳
Original assignee: Fourth Military Medical University FMMU
Current assignee: Fourth Military Medical University FMMU
Priority date: 2007-05-11
Filing date: 2007-05-11
Publication date: 2008-05-14
Anticipated expiration: 2017-05-11

Abstract

In order to make up for the deficiency that the cellulose acetate membrane electrophoresis tank and the agarose gel electrophoresis tank in the prior education are single in respective structures and functions, the utility model discloses a bifunctional electrophoresis tank, comprising a box body, wherein, two sides inside the box body are symmetrically provided with two electrolyte cavities with the same volume which are separated by two symmetrical concave isolating walls; a rectangular concave cavity positioned on the very middle part inside the box body are arranged between two symmetric concave isolating walls; at least one removable pectinate sheet is arranged on the length direction of the rectangular concave cavity; comb lugs are respectively extended from two ends of the pectinate sheet and are just respectively arranged across two opposite sides of the box body; comb teeth of the pectinate sheet are opposite to the bottom surface of the rectangular concave cavity. The electrophoresis tank of the utility model can simultaneously meet the needs of cellulose acetate membrane electrophoresis experiments and agarose gel electrophoresis experiments.

Description

A kind of difunctional electrophoresis chamber

Technical field

The utility model relates to a kind of cellulose acetate liquo and the used difunctional electrophoresis chamber of agarose gel electrophoresis test.

Background technology

Electrophoretic technique is widely used in many fields such as fundamental research, agricultural sciences, medical and health, industrial production, national defence scientific research, medical jurisprudence and commodity inspection.A kind of especially indispensable important analysis means in molecular biology research work.

Nucleotide, protein (comprising enzyme and isozyme) and polypeptide and amino acid etc. all have ionogenic group, and group can absorb or provide hydrogen ion in solution, thereby becomes charge particle; Because more or less and molecule with identical charges varies for charge particle, so in different ionogen, under electric field influence, the speed that they move is also inequality.People utilize this specific character, with electrophoretic method above-mentioned substance are carried out qualitative and quantitative analysis, and perhaps certain mixture separation are become each component and do a small amount of electrophoresis preparation.

Agarose gel electrophoresis is suitable for the separating of immunocomplex, nucleic acid and nucleoprotein, evaluation and purifying.In the clinical biochemical check, be usually used in detection carrier as LDH (serum lactic dehydrogenase), CK isozymes such as (creatine kinases).

The cellulose acetate that cellulose acetate liquo adopted is the cellulose acetate of glycoloyl formation.The film of being made by this material is called cellulose acetate film.This film is little to the protein example adsorptivity, almost can eliminate " hangover " phenomenon that occurs in the paper electrophoresis fully, again because the wetting ability of film is smaller, the damping fluid that it held is also few, most of electric current is conducted by sample during cellulose acetate liquo, so velocity of separation is fast, electrophoresis time is short, amount of samples is few, and the protein of 5 μ g can obtain satisfied separating effect.Therefore, be suitable for the detection of micro-paraprotein under the pathologic condition.

At present, in the electrophoresis experiment teaching that utilizes sepharose and cellulose acetate as the molecular biology carrier, because the difference of two kinds of carriers, used electrophoresis experiment needs different devices.As the electrophoresis experiment of Dichlorodiphenyl Acetate fiber membrane, the structure of its electrophoresis chamber can not be used for the electrophoresis experiment of sepharose; And the structure of agarose gel electrophoresis groove only is used for the agarose gel electrophoresis experiment, so, in student's education experiment, original two kinds of electrophoresis chamber 26S Proteasome Structure and Functions are single, when carrying out above-mentioned two kinds of electrophoresis test, must buy two kinds of electrophoresis chambers respectively and could satisfy test requirements document, make experimentation cost strengthen, the laboratory takes up space big and random.

Summary of the invention

In order to remedy two kinds of single deficiencies of electrophoresis chamber structure in the existing teaching, the utility model provides a kind of difunctional electrophoresis chamber, promptly designs the needs that an electrophoresis chamber just can satisfy the electrophoresis experiment of the electrophoresis experiment of acetate film and sepharose simultaneously.

For reaching above purpose, the utility model takes following technical scheme to be achieved:

A kind of difunctional electrophoresis chamber, comprise a box body, zygomorphy is provided with the electrolyte cavities of two same volumes in the box body, it is separated by two symmetric spill divider walls, it is characterized in that, be provided with a rectangle cavity that is positioned at the positive middle part of box body between described two symmetric spill divider walls, on the length direction of this rectangle cavity, be provided with a removable pectination sheet at least, these pectination sheet two ends are extended with the comb ear, it just in time distinguishes frame on two opposite side of described box body, the relative rectangle cavity of the broach of this pectination sheet bottom surface.

In the such scheme, described box body is rectangle or the square uncovered box body that transparent organic glass is made; The broach of described pectination sheet is the rectangle broach.

The utility model adopts being positioned at the positive middle part of box body the rectangle cavity is set, and shelve the pectination sheet and can in the rectangle cavity, water sepharose, carry out the electrophoresis experiment of sepharose carrier, if remove the electrophoresis experiment that the pectination sheet just can carry out acetate film at once simply, thereby can realize that a box is dual-purpose, the test work amount was reduced, can save experiment fees again.

Description of drawings

Fig. 1 is a structure iron of the present utility model.Wherein: 1, box body; 2, wire electrode; 3, electrode terminal; 4, electrolyte cavities; 5, spill divider wall; 6, rectangle cavity; 7, pectination sheet.

Fig. 2 is the structure iron of pectination sheet among Fig. 1.Wherein: 8, comb ear; 9, broach.

Fig. 3 is the state graph when removing the pectination sheet among Fig. 1.

Embodiment

The utility model is described in further detail below in conjunction with drawings and Examples.

Embodiment 1

As shown in Figure 1 and Figure 2, a kind of electrophoresis chamber that can be used for carrying out agarose gel electrophoresis, it comprises a rectangular box 1, zygomorphy is provided with the electrolyte cavities 4 of two same volumes in the box body 1, it is separated by two symmetric spill divider walls 5, the box body 1 inboard top of each electrolyte cavities 4 vertical end is provided with electrode terminal 3 to box body 1, each electrolyte cavities 4 bottom surface is covered with a wire electrode 2 and connects its corresponding electrode terminal 3, one of them connects positive source, and another connects power cathode.Rectangular box 1 adopts transparent organic glass to make, and can clearly observe some variations in detail in the electrophoresis process like this in experiment.

Article two, be provided with a rectangle cavity 6 that is positioned at box body 1 positive middle part between the symmetric spill divider wall 5, on the length direction of this rectangle cavity 6, be provided with a pectination sheet 7, these pectination sheet 7 two ends are extended with comb ear 8, it just in time distinguishes frame on two opposite side of described box body, the bottom surface of the broach 9 relative rectangle cavitys 6 of

pectination sheet

7,7 width positions on rectangle cavity 6 of pectination sheet are adjustable, and broach 9 can be made rectangle or other shape according to the experiment needs.

During electrophoresis experiment,, slowly pour cavity 6 into, until forming layer of even glue face with about 60 ℃ sepharose liquid; After treating that gelling is solid, extract pectination sheet 7 carefully, form a gang saw tooth sample well on the gel, formed sample well should be near electrode terminal 3 negative potentials one end.With liquid-transfering gun the DNA sample is clicked and entered sample well then and write down point sample order and the point sample amount, electrophoretic buffer is slowly added in the electrolyte cavities 4 again, liquid volume added will make damping fluid cross spill divider wall 5 to liquid level not have glue face 1-1.5 millimeter.Connect the power supply of electrode terminal 3 and electrophoresis apparatus at last.Because the travelling speed of DNA is directly proportional with strength of electric field, general strength of electric field does not surpass 5V/cm.

Embodiment 2

As shown in Figure 3, another kind can be used for carrying out the electrophoresis chamber of acetate film, it is to remove pectination sheet 7 on the basis of embodiment 1 structure, during electrophoresis experiment, just commercially available cellulose acetate diaphragm is soaked in damping fluid earlier, after soaking into, take out diaphragm and remove unnecessary damping fluid with the filter paper suction, electrophoretic buffer added to be no more than spill divider wall 5 in the electrolyte cavities 4, electrophoretic buffer is the barbitol buffer solution of pH8.6, concentration strides across two spill divider walls 5 with set acetate film bar level then at 0.05-0.09mol/L, and film bar two ends are immersed respectively in the damping fluid of electrolyte cavities 4, on the acetate film bar, drip sample again, connect the power supply of electrode terminal 3 and electrophoresis apparatus at last, voltage is 25V/cm, and electric current is 0.4-0.6mA/cm.

Claims

1. difunctional electrophoresis chamber, comprise a box body, zygomorphy is provided with the electrolyte cavities of two same volumes in the box body, it is separated by two symmetric spill divider walls, it is characterized in that, be provided with a rectangle cavity that is positioned at the positive middle part of box body between described two symmetric spill divider walls, on the length direction of this rectangle cavity, be provided with a removable pectination sheet at least, these pectination sheet two ends are extended with the comb ear, it just in time distinguishes frame on two opposite side of described box body, the bottom surface of the relative rectangle cavity of the broach of this pectination sheet.

2. difunctional electrophoresis chamber according to claim 1 is characterized in that, described box body is rectangle or the square uncovered box body that transparent organic glass is made.

3. difunctional electrophoresis chamber according to claim 1 is characterized in that, the broach of described pectination sheet is the rectangle broach.