CN103808941A - Multifunctional protein reaction experiment device - Google Patents

Multifunctional protein reaction experiment device Download PDF

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Publication number
CN103808941A
CN103808941A CN201410041596.XA CN201410041596A CN103808941A CN 103808941 A CN103808941 A CN 103808941A CN 201410041596 A CN201410041596 A CN 201410041596A CN 103808941 A CN103808941 A CN 103808941A
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China
Prior art keywords
box body
rotating shaft
gear
compressor arm
upper cover
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Granted
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CN201410041596.XA
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Chinese (zh)
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CN103808941B (en
Inventor
高社干
时振国
朱小娟
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First Affiliated Hospital of Henan University of Science and Technology
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First Affiliated Hospital of Henan University of Science and Technology
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Priority to CN201410041596.XA priority Critical patent/CN103808941B/en
Publication of CN103808941A publication Critical patent/CN103808941A/en
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

The invention relates to a multifunctional protein reaction experiment device which comprises a box body, an upper cover as well as a pressing mechanism I and a pressing mechanism II, wherein the upper end of the box body is opened; the pressing mechanism I is arranged on the box body; the pressing mechanism II is arranged on the upper cover; the distance between two pressing rods I of the pressing mechanism I can be adjusted through an adjusting device I; the distance between two pressing rods II of the pressing mechanism II can be adjusted through an adjusting device II. By adopting the multifunctional protein reaction experiment device provided by the invention, a PVDF (Polyvinylidene Fluoride) film can be fixed in an antibody solution to be sufficiently contacted with an antibody for reaction, and an reagent can be saved, so that the experiment device is applicable to PVDF films with various models, and moreover the film is not damaged in the whole experiment process; an adjusting method is simple and feasible, pollution to the environment and air caused by a disposable plastic bag and a heat sealer is avoided, and moreover great repeatability is achieved, the structural design is smart, and the experiment cost is greatly lowered.

Description

A kind of multifunctional protein reaction experiment device
Technical field
The present invention relates to medical science, biomolecular science experiment apparatus technical field, be specifically related to a kind of multifunctional protein reaction experiment device.
Background technology
Protein blot experiment (being Western blot) is medical science and the conventional experimental technique of molecular biosciences cytology, wherein the PVDF membrane after transferring film (pvdf membrane) or nitrocellulose filter (NC film) etc. are the committed step of experiment with antibody incubation, by albumen-antibody incubation technology be now: the pvdf membrane of the albumen that takes a turn for the better or NC film are placed in the polybag of cutting off, add after an antiantibody, catch up with after the interior gas of most bag or bubble, sealed with capper, be placed in 4 ℃ of refrigerator overnight incubation, two anti-also need again with new polybag, NC film or pvdf membrane to be hatched.But because pvdf membrane or NC film density are less than the density of antibody-solutions, easily floating in antibody-solutions, cause the albumen on pvdf membrane or NC film to contact inhomogeneous with antibody, in addition, pvdf membrane or the tender easy scuffing in surface of NC film, and when sealing, drive the bothersome effort of gas in bag, if residual gas also may cause pvdf membrane or NC film to contact insufficient with antibody, interference experiment result, the sensitivity of experiment is declined, the accuracy of impact experiment, make the failure of an experiment, waste of manpower, material resources, financial resources, and repeatedly use the disposable plastic bag also can be to environment.
Summary of the invention
The object of the invention is the deficiency for solving the problems of the technologies described above, a kind of multifunctional protein reaction experiment device is provided, pvdf membrane or NC film can be fixed in antibody-solutions, make its abundant and antibody contact reaction, and can save reagent, but also reusable, avoid disposable plastic bag pollution on the environment, obviously reduce experimental cost.
The present invention is the deficiency solving the problems of the technologies described above, the technical scheme adopting is: a kind of multifunctional protein reaction experiment device, comprise box body and the upper cover of upper end open, described albumino reaction box also includes the hold-down mechanism I being arranged on box body and is arranged at the hold-down mechanism II of upper cover, hold-down mechanism I comprises regulating device I, two toothed rack I, two connecting rod I and two compressor arm I, described regulating device I comprises rotating shaft I, gear I and thumb wheel I, rotating shaft I is located on box body one side sidewall, rotating shaft I can rotate freely, one end that described rotating shaft I is positioned at box body inside connects gear I, one end that rotating shaft I is positioned at box body outside connects thumb wheel I, the above and below of gear I is respectively equipped with a tooth bar I, two tooth bar I are all intermeshing with gear I, described two tooth bar I are all arranged on the sidewall of box body by chute, two tooth bar I are all parallel to box bottom setting, described two toothed rack I are all connected with compressor arm I by connecting rod I, described compressor arm I has one section of depressed part, the opening of depressed part is consistent with the opening direction of box body, in depressed part, be provided with fagging, between fagging and the bottom surface of depressed part, be provided with multiple springs, two bottom surface settings that compressor arm I is parallel to each other and presses close to box body,
Described hold-down mechanism II comprises regulating device II, tooth bar II, connecting rod II and compressor arm II, regulating device II comprises rotating shaft II, gear II and thumb wheel II, rotating shaft II is through upper cover setting, and rotating shaft II can rotate freely, one end that rotating shaft II is positioned at upper cover inside arranges gear II, one end that rotating shaft II is positioned at upper cover outside arranges thumb wheel II, the both sides of gear II are provided with the two toothed rack II that are parallel to each other, two toothed rack II are all intermeshing with gear II, described two tooth bar II are all arranged on the lid of upper cover by chute, two toothed rack II are all connected with compressor arm II by connecting rod II, two compressor arm II are parallel to each other and perpendicular to the sidewall setting of box body.
beneficial effect
Multifunctional protein reaction experiment device of the present invention, the film of the different materials such as pvdf membrane or NC film can be fixed in antibody-solutions, make its abundant and antibody contact reaction, and can save reagent, be particularly useful for the film of the difference size after fixing cutting, and to film not damaged, its control method is simple and easy to do, more avoided being suitable for the pollution that disposable plastic bag and heat sealing machine cause environment and air, and Reusability is strong, structural design is ingenious, greatly reduces experimental cost.
Accompanying drawing explanation
Fig. 1 is external structure schematic diagram of the present invention;
Fig. 2 is the structural representation of hold-down mechanism I of the present invention;
Fig. 3 is the structural representation of regulating device I of the present invention;
Fig. 4 is the structural representation of hold-down mechanism II of the present invention;
Fig. 5 is the structural representation of regulating device II of the present invention and connecting rod II;
Fig. 6 is the structural representation of compressor arm I of the present invention;
Mark in figure: 1, box body, 2, upper cover, 301, regulating device I, 302, tooth bar I, 303, connecting rod I, 304, compressor arm I, 3011, rotating shaft I, 3012, gear I, 3013, thumb wheel I, 3041, depressed part, 3042, fagging, 3043, spring, 401, regulating device II, 402, tooth bar II, 403, connecting rod II, 404, compressor arm II, 4011, rotating shaft II, 4012, gear II, 4013, thumb wheel II.
Embodiment
As shown in the figure: a kind of multifunctional protein reaction experiment device, comprise box body 1 and the upper cover 2 of upper end open, described albumino reaction box also includes the hold-down mechanism I being arranged on box body 1 and is arranged at the hold-down mechanism II of upper cover 2, hold-down mechanism I comprises regulating device I 301, two toothed rack I 302, two connecting rod I 303 and two compressor arm I 304, regulating device I 301 is used for regulating two distances between compressor arm I 304, adjusts according to the pvdf membrane of different sizes.Described regulating device I 301 comprises rotating shaft I 3011, gear I 3012 and thumb wheel I 3013, rotating shaft I 3011 is located on box body 1 one side sidewalls, rotating shaft I 3011 can rotate freely, one end that described rotating shaft I 3011 is positioned at box body 1 inside connects gear I 3012, one end that rotating shaft I 3011 is positioned at box body 1 outside connects thumb wheel I 3013, stirring thumb wheel I 3013 can make gear I 3012 rotate, the above and below of gear I 3012 is respectively equipped with a tooth bar I 302, the engaging tooth that is positioned at the tooth bar I 302 of top arranges down, the engaging tooth that is positioned at the tooth bar I 302 of below arranges upward.Two tooth bar I 302 are all intermeshing with gear I 3012, and described two tooth bar I 302 are all arranged on the sidewall of box body 1 by chute.Two tooth bar I 302 are all fastened in chute, and tooth bar I 302 can not deviate from from chute, and tooth bar I 302 can be along the length direction displacement of chute.Two tooth bar I 302 are all parallel to box body 1 bottom surface and arrange, described two toothed rack I 302 are all connected with compressor arm I 304 by connecting rod I 303, described compressor arm I 304 has one section of depressed part 3041, the opening of depressed part 3041 is consistent with the opening direction of box body 1, in depressed part 3041, be provided with fagging 3042, between the bottom surface of fagging 3042 and depressed part 3041, be provided with 3043, two compressor arm I 304 of multiple springs be separately positioned on two tooth bar I 302 away from end.Two compressor arm I 304 are parallel to each other and press close to box body 1 bottom surface and arrange;
Described hold-down mechanism II comprises regulating device II 401, tooth bar II 402, connecting rod II 403 and compressor arm II 404, and regulating device II 401 is used for regulating two distances between compressor arm II 404, adjusts according to the pvdf membrane of different sizes.Regulating device II 401 comprises rotating shaft II 4011, gear II 4012 and thumb wheel II 4013, rotating shaft II 4011 arranges through upper cover 2, and rotating shaft II 4011 can rotate freely, one end that rotating shaft II 4011 is positioned at upper cover 2 inside arranges gear II 4012, one end that rotating shaft II 4011 is positioned at upper cover 2 outsides arranges thumb wheel II 4013, stirring thumb wheel II 4013 can make gear II 4012 rotate, the both sides of gear II 4012 are provided with the two toothed rack II 402 that are parallel to each other, two toothed rack II 402 are all intermeshing with gear II 4012, described two tooth bar II 402 are all arranged on the lid of upper cover 2 by chute, two tooth bar II 402 are all fastened in chute, tooth bar II 402 can not deviate from from chute, and tooth bar II 402 can be along the length direction displacement of chute.Two toothed rack II 402 are all connected with by connecting rod II 403 that 404, two compressor arm II 404 of compressor arm II are parallel to each other and perpendicular to the sidewall setting of box body 1.
When use, the size of the pvdf membrane of operation as required, adjust two distances between compressor arm I 304 by regulating device I 301, stirring thumb wheel I 3013 rotates gear I 3012, 3012 rotations of gear I make two toothed rack I 302 relative displacements that are engaged with, just make 304 relative displacements simultaneously of two compressor arm I, shorten the distance between two compressor arm I 304, after adjustment finishes, pvdf membrane is placed on the fagging 3042 of two compressor arm I 304, then adjust two distances between compressor arm II 404 by regulating device II 401, stirring thumb wheel II 4013 rotates gear II 4012, 4012 rotations of gear II make two toothed rack II 402 relative displacements that are engaged with, just make 404 relative displacements simultaneously of two compressor arm II, shorten the distance between two compressor arm II 404, after adjustment finishes, upper cover 2 is covered and makes two compressor arm I 304 and two compressor arm II 404 intersect to form " well " font, pvdf membrane is clipped in the middle and is fixed, because pvdf membrane is placed on fagging 3042, fagging 3042 is due to the effect of spring 3043, its height can be adjusted voluntarily, therefore existing certain adjustment space, pvdf membrane can be compressed again.Multifunctional protein reaction experiment device of the present invention, can be fixed on pvdf membrane in antibody-solutions, makes it fully and antibody contact reaction, and can save reagent.
Further on upper cover 2, offer rectangular through slot, in this rectangular through slot, be embedded transparent rule, rule is mutually vertical with two compressor arm I 304, can see through transparent rule and observe the distance between two compressor arm I 304, realize precisely and regulating, be applicable to the pvdf membrane of plurality of specifications at this experimental provision, and can not cause damage to film in whole experimentation, control method is simple and easy to do, more avoid being suitable for the pollution that disposable plastic bag and heat sealing machine cause environment and air, and repeatability is strong, structural design is ingenious, greatly reduce experimental cost.

Claims (1)

1. a multifunctional protein reaction experiment device, comprise box body (1) and the upper cover (2) of upper end open, it is characterized in that: described albumino reaction box also includes the hold-down mechanism I being arranged on box body (1) and is arranged at the hold-down mechanism II of upper cover (2), hold-down mechanism I comprises regulating device I (301), two toothed rack I (302), two connecting rod I (303) and two compressor arm I (304), described regulating device I (301) comprises rotating shaft I (3011), gear I (3012) and thumb wheel I (3013), rotating shaft I (3011) is located on box body (1) one side sidewall, rotating shaft I (3011) can rotate freely, described rotating shaft I (3011) is positioned at the inner one end of box body (1) and connects gear I (3012), rotating shaft I (3011) is positioned at the outside one end of box body (1) and connects thumb wheel I (3013), the above and below of gear I (3012) is respectively equipped with a tooth bar I (302), two tooth bar I (302) are all intermeshing with gear I (3012), described two tooth bar I (302) are all arranged on the sidewall of box body (1) by chute, two tooth bar I (302) are all parallel to box body (1) bottom surface and arrange, described two toothed rack I (302) are all connected with compressor arm I (304) by connecting rod I (303), described compressor arm I (304) has one section of depressed part (3041), the opening of depressed part (3041) is consistent with the opening direction of box body (1), in depressed part (3041), be provided with fagging (3042), between the bottom surface of fagging (3042) and depressed part (3041), be provided with multiple springs (3043), two compressor arm I (304) are parallel to each other and press close to box body (1) bottom surface and arrange,
Described hold-down mechanism II comprises regulating device II (401), tooth bar II (402), connecting rod II (403) and two compressor arm II (404), regulating device II (401) comprises rotating shaft II (4011), gear II (4012) and thumb wheel II (4013), rotating shaft II (4011) arranges through upper cover (2), and rotating shaft II (4011) can rotate freely, rotating shaft II (4011) is positioned at the inner one end of upper cover (2) gear II (4012) is set, rotating shaft II (4011) is positioned at the outside one end of upper cover (2) thumb wheel II (4013) is set, the both sides of gear II (4012) are provided with the two toothed rack II (402) that are parallel to each other, two toothed rack II (402) are all intermeshing with gear II (4012), described two tooth bar II (402) are all arranged on the lid of upper cover (2) by chute, two toothed rack II (402) are all connected with compressor arm II (404) by connecting rod II (403), two compressor arm II (404) are parallel to each other and perpendicular to the sidewall setting of box body (1).
CN201410041596.XA 2014-01-28 2014-01-28 A kind of multifunctional protein reaction experiment device Expired - Fee Related CN103808941B (en)

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Application Number Priority Date Filing Date Title
CN201410041596.XA CN103808941B (en) 2014-01-28 2014-01-28 A kind of multifunctional protein reaction experiment device

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Application Number Priority Date Filing Date Title
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CN103808941A true CN103808941A (en) 2014-05-21
CN103808941B CN103808941B (en) 2015-08-26

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN201569655U (en) * 2009-12-17 2010-09-01 东莞市兴仁保健食品有限公司 Immunoblot antibody incubation box
CN201583540U (en) * 2010-01-08 2010-09-15 陈锦 Blotting incubation box
CN202305534U (en) * 2011-11-14 2012-07-04 北京康为世纪生物科技有限公司 Western blotting antibody incubation box
US8470541B1 (en) * 2008-09-27 2013-06-25 Boston Heart Diagnostics Corporation Methods for separation and immuno-detection of biomolecules, and apparatus related thereto

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8470541B1 (en) * 2008-09-27 2013-06-25 Boston Heart Diagnostics Corporation Methods for separation and immuno-detection of biomolecules, and apparatus related thereto
CN201569655U (en) * 2009-12-17 2010-09-01 东莞市兴仁保健食品有限公司 Immunoblot antibody incubation box
CN201583540U (en) * 2010-01-08 2010-09-15 陈锦 Blotting incubation box
CN202305534U (en) * 2011-11-14 2012-07-04 北京康为世纪生物科技有限公司 Western blotting antibody incubation box

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