CN103808941B - A kind of multifunctional protein reaction experiment device - Google Patents
A kind of multifunctional protein reaction experiment device Download PDFInfo
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- CN103808941B CN103808941B CN201410041596.XA CN201410041596A CN103808941B CN 103808941 B CN103808941 B CN 103808941B CN 201410041596 A CN201410041596 A CN 201410041596A CN 103808941 B CN103808941 B CN 103808941B
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- box body
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
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Abstract
The present invention relates to a kind of multifunctional protein reaction experiment device, comprise box body and the upper cover of upper end open, described albumino reaction box also includes the hold-down mechanism I be arranged on box body and the hold-down mechanism II being arranged at upper cover, distance between two compressor arms I of hold-down mechanism I regulates by regulating device I, distance between two compressor arms II of hold-down mechanism II regulates by regulating device II, multifunctional protein reaction experiment device of the present invention, can pvdf membrane be fixed in antibody-solutions, make its fully and antibody contacts react, and can reagent be saved, this experimental provision is applicable to the pvdf membrane of plurality of specifications, and damage can not be caused to film in whole experimentation, control method is simple and easy to do, more avoid the pollution being suitable for disposable plastic bag and heat sealing machine and environment and air are caused, and repeatability is strong, smart structural design, greatly reduce experimental cost.
Description
Technical field
The present invention relates to medical science, biomolecular science experiment apparatus technical field, be specifically related to a kind of multifunctional protein reaction experiment device.
Background technology
The experimental technique that protein blot experiment (i.e. Western blot) is commonly used for medical science and molecular biosciences cytology, PVDF membrane (pvdf membrane) wherein after transferring film or nitrocellulose filter (NC film) etc. are the committed step of testing with antibody incubation, by the Protein-antibody technology of hatching be now: the pvdf membrane of the albumen that takes a turn for the better or NC film are placed in the polybag cut off, after adding an antiantibody, after catching up with in most bag gas or bubble, sealed with capper, be placed in 4 DEG C of refrigerator overnight incubation, two anti-also need again to hatch NC film or pvdf membrane with new polybag.But because pvdf membrane or NC film density are less than the density of antibody-solutions, easily floating in antibody-solutions, cause the albumen on pvdf membrane or NC film and antibody contacts uneven, in addition, pvdf membrane or the tender easy scuffing in surface of NC film, and the bothersome effort of gas of driveing during sealing in bag, if residual gas, pvdf membrane or NC film and antibody contacts also may be caused insufficient, interference experiment result, the sensitivity of experiment is declined, the accuracy of impact experiment, make the failure of an experiment, waste of manpower, material resources, financial resources, and repeatedly use disposable plastic bag also can to environment.
Relate art literature:
Patent documentation 1:CN201583540U, it discloses a kind of blotting incubation box;
Patent documentation 2:CN202305534U, it discloses a kind of western blotting antibody incubation box;
Patent documentation 3:CN201569655U, it discloses a kind of western blotting antibody incubation box.
Summary of the invention
The object of the invention is the deficiency for solving the problems of the technologies described above, a kind of multifunctional protein reaction experiment device is provided, can pvdf membrane or NC film be fixed in antibody-solutions, make its fully and antibody contacts react, and can reagent be saved, but also reusable, avoid disposable plastic bag pollution on the environment, obviously reduce experimental cost.
The present invention is the deficiency solved the problems of the technologies described above, the technical scheme adopted is: a kind of multifunctional protein reaction experiment device, comprise box body and the upper cover of upper end open, described albumino reaction box also includes the hold-down mechanism I be arranged on box body and the hold-down mechanism II being arranged at upper cover, hold-down mechanism I comprises regulating device I, two toothed rack I, two connecting rods I and two compressor arms I, described regulating device I comprises rotating shaft I, gear I and thumb wheel I, rotating shaft I is located on the sidewall of box body side, rotating shaft I can rotate freely, one end that described rotating shaft I is positioned at tray interior connects gear I, one end that rotating shaft I is positioned at box portion outside connects thumb wheel I, a tooth bar I is respectively equipped with above and below gear I, two tooth bars I all engage each other with gear I, described two tooth bars I are all arranged on the sidewall of box body by chute, two tooth bars I are all parallel to box bottom and arrange, described two toothed rack I are all connected with compressor arm I by connecting rod I, described compressor arm I has one section of depressed part, the opening of depressed part is consistent with the opening direction of box body, fagging is provided with in depressed part, multiple spring is provided with between fagging and the bottom surface of depressed part, two compressor arms I are parallel to each other and press close to the bottom surface setting of box body,
Described hold-down mechanism II comprises regulating device II, tooth bar II, connecting rod II and compressor arm II, regulating device II comprises rotating shaft II, gear II and thumb wheel II, rotating shaft II is arranged through upper cover, and rotating shaft II can rotate freely, one end that rotating shaft II is positioned at upper cover inside arranges gear II, one end that rotating shaft II is positioned at upper cover outside arranges thumb wheel II, the both sides of gear II are provided with two toothed rack II be parallel to each other, two toothed rack II all engage each other with gear II, described two tooth bars II are all arranged on the lid of upper cover by chute, two toothed rack II are all connected with compressor arm II by connecting rod II, two compressor arms II are parallel to each other and sidewall perpendicular to box body is arranged.
beneficial effect
Multifunctional protein reaction experiment device of the present invention, can the film of the different materials such as pvdf membrane or NC film be fixed in antibody-solutions, make its fully and antibody contacts react, and can reagent be saved, be particularly useful for the film of the different sizes after fixing cutting, and to film not damaged, its control method is simple and easy to do, more avoid the pollution being suitable for disposable plastic bag and heat sealing machine and environment and air are caused, and Reusability is strong, smart structural design, greatly reduces experimental cost.
Accompanying drawing explanation
Fig. 1 is external structure schematic diagram of the present invention;
Fig. 2 is the structural representation of hold-down mechanism I of the present invention;
Fig. 3 is the structural representation of regulating device I of the present invention;
Fig. 4 is the structural representation of hold-down mechanism II of the present invention;
Fig. 5 is the structural representation of regulating device II of the present invention and connecting rod II;
Fig. 6 is the structural representation of compressor arm I of the present invention;
Mark in figure: 1, box body, 2, upper cover, 301, regulating device I, 302, tooth bar I, 303, connecting rod I, 304, compressor arm I, 3011, rotating shaft I, 3012, gear I, 3013, thumb wheel I, 3041, depressed part, 3042, fagging, 3043, spring, 401, regulating device II, 402, tooth bar II, 403, connecting rod II, 404, compressor arm II, 4011, rotating shaft II, 4012, gear II, 4013, thumb wheel II.
Embodiment
As shown in the figure: a kind of multifunctional protein reaction experiment device, comprise box body 1 and the upper cover 2 of upper end open, described albumino reaction box also includes the hold-down mechanism I be arranged on box body 1 and the hold-down mechanism II being arranged at upper cover 2, hold-down mechanism I comprises regulating device I 301, two toothed rack I 302, two connecting rods I 303 and two compressor arms I 304, regulating device I 301 is used for the distance between adjustment two compressor arms I 304, and the pvdf membrane according to different size adjusts.Described regulating device I 301 comprises rotating shaft I 3011, gear I 3012 and thumb wheel I 3013, rotating shaft I 3011 is located on the sidewall of box body 1 side, rotating shaft I 3011 can rotate freely, one end that described rotating shaft I 3011 is positioned at box body 1 inside connects gear I 3012, one end that rotating shaft I 3011 is positioned at box body 1 outside connects thumb wheel I 3013, stirring thumb wheel I 3013 can make gear I 3012 rotate, a tooth bar I 302 is respectively equipped with above and below gear I 3012, namely the engaging tooth being positioned at the tooth bar I 302 of top is arranged down, the engaging tooth being positioned at the tooth bar I 302 of below is arranged upward.Two tooth bars I 302 all engage each other with gear I 3012, and described two tooth bars I 302 are all arranged on by chute on the sidewall of box body 1.Two tooth bars I 302 are all fastened in chute, and tooth bar I 302 can not be deviate from from chute, and tooth bar I 302 can along the length direction displacement of chute.Two tooth bars I 302 are all parallel to box body 1 bottom surface and arrange, described two toothed rack I 302 are all connected with compressor arm I 304 by connecting rod I 303, described compressor arm I 304 has one section of depressed part 3041, the opening of depressed part 3041 is consistent with the opening direction of box body 1, fagging 3042 is provided with in depressed part 3041, be provided with between the bottom surface of fagging 3042 and depressed part 3041 multiple spring 3043, two compressor arms I 304 be separately positioned on two tooth bars I 302 away from end.Two compressor arms I 304 are parallel to each other and press close to box body 1 bottom surface and arrange;
Described hold-down mechanism II comprises regulating device II 401, tooth bar II 402, connecting rod II 403 and compressor arm II 404, and regulating device II 401 is used for the distance between adjustment two compressor arms II 404, and the pvdf membrane according to different size adjusts.Regulating device II 401 comprises rotating shaft II 4011, gear II 4012 and thumb wheel II 4013, rotating shaft II 4011 is arranged through upper cover 2, and rotating shaft II 4011 can rotate freely, one end that rotating shaft II 4011 is positioned at upper cover 2 inside arranges gear II 4012, one end that rotating shaft II 4011 is positioned at upper cover 2 outside arranges thumb wheel II 4013, stirring thumb wheel II 4013 can make gear II 4012 rotate, the both sides of gear II 4012 are provided with two toothed rack II 402 be parallel to each other, two toothed rack II 402 all engage each other with gear II 4012, described two tooth bars II 402 are all arranged on by chute on the lid of upper cover 2, two tooth bars II 402 are all fastened in chute, tooth bar II 402 can not be deviate from from chute, and tooth bar II 402 can along the length direction displacement of chute.Two toothed rack II 402 are all connected with compressor arm II 404 by connecting rod II 403, and two compressor arms II 404 are parallel to each other and sidewall perpendicular to box body 1 is arranged.
During use, the size of the pvdf membrane operated as required, the distance between two compressor arms I 304 is adjusted by regulating device I 301, namely stirring thumb wheel I 3013 makes gear I 3012 rotate, gear I 3012 rotates two toothed rack I 302 relative displacements making to be engaged with, just make the relative displacement simultaneously of two compressor arms I 304, shorten the distance between two compressor arms I 304, on the fagging 3042 after adjustment terminates pvdf membrane being placed in two compressor arms I 304, then the distance between two compressor arms II 404 is adjusted by regulating device II 401, namely stirring thumb wheel II 4013 makes gear II 4012 rotate, gear II 4012 rotates two toothed rack II 402 relative displacements making to be engaged with, just make the relative displacement simultaneously of two compressor arms II 404, shorten the distance between two compressor arms II 404, after adjustment terminates, upper cover 2 is covered and makes two compressor arms I 304 and two compressor arms II 404 intersect to form " well " font, pvdf membrane is clipped in the middle and is fixed, because pvdf membrane is placed on fagging 3042, fagging 3042 is due to the effect of spring 3043, it highly can from Row sum-equal matrix, therefore existing adjustment space necessarily, pvdf membrane can be compressed again.Multifunctional protein reaction experiment device of the present invention, can be fixed in antibody-solutions by pvdf membrane, make its fully and antibody contacts react, and can reagent be saved.
Further upper cover 2 offers rectangular through slot, transparent rule is embedded in this rectangular through slot, rule is mutually vertical with two compressor arms I 304, the distance between two compressor arms I 304 can be observed through transparent rule, realize precisely regulating, the pvdf membrane of plurality of specifications is applicable at this experimental provision, and damage can not be caused to film in whole experimentation, control method is simple and easy to do, more avoid the pollution being suitable for disposable plastic bag and heat sealing machine and environment and air are caused, and repeatability is strong, smart structural design, greatly reduce experimental cost.
Claims (1)
1. a multifunctional protein reaction experiment device, comprise box body (1) and the upper cover (2) of upper end open, it is characterized in that: described albumino reaction box also includes the hold-down mechanism I be arranged on box body (1) and the hold-down mechanism II being arranged at upper cover (2), hold-down mechanism I comprises regulating device I (301), two toothed rack I (302), two connecting rods I (303) and two compressor arms I (304), described regulating device I (301) comprises rotating shaft I (3011), gear I (3012) and thumb wheel I (3013), rotating shaft I (3011) is located on the sidewall of box body (1) side, rotating shaft I (3011) can rotate freely, described rotating shaft I (3011) is positioned at the inner one end of box body (1) and connects gear I (3012), rotating shaft I (3011) is positioned at the outside one end of box body (1) and connects thumb wheel I (3013), a tooth bar I (302) is respectively equipped with above and below gear I (3012), two tooth bars I (302) all engage each other with gear I (3012), described two tooth bars I (302) are all arranged on the sidewall of box body (1) by chute, two tooth bars I (302) are all parallel to box body (1) bottom surface and arrange, described two toothed rack I (302) are all connected with compressor arm I (304) by connecting rod I (303), described compressor arm I (304) has one section of depressed part (3041), the opening of depressed part (3041) is consistent with the opening direction of box body (1), fagging (3042) is provided with in depressed part (3041), multiple spring (3043) is provided with between the bottom surface of fagging (3042) and depressed part (3041), two compressor arms I (304) are parallel to each other and press close to box body (1) bottom surface and arrange,
Described hold-down mechanism II comprises regulating device II (401), two toothed rack II (402), connecting rod II (403) and two compressor arms II (404), regulating device II (401) comprises rotating shaft II (4011), gear II (4012) and thumb wheel II (4013), rotating shaft II (4011) is arranged through upper cover (2), and rotating shaft II (4011) can rotate freely, one end that rotating shaft II (4011) is positioned at upper cover (2) inner arranges gear II (4012), one end that rotating shaft II (4011) is positioned at upper cover (2) outside arranges thumb wheel II (4013), the both sides of gear II (4012) are provided with two toothed rack II (402) be parallel to each other, two toothed rack II (402) all engage each other with gear II (4012), described two tooth bars II (402) are all arranged on the lid of upper cover (2) by chute, two toothed rack II (402) are all connected with compressor arm II (404) by connecting rod II (403), two compressor arms II (404) are parallel to each other and sidewall perpendicular to box body (1) is arranged.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410041596.XA CN103808941B (en) | 2014-01-28 | 2014-01-28 | A kind of multifunctional protein reaction experiment device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410041596.XA CN103808941B (en) | 2014-01-28 | 2014-01-28 | A kind of multifunctional protein reaction experiment device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103808941A CN103808941A (en) | 2014-05-21 |
| CN103808941B true CN103808941B (en) | 2015-08-26 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410041596.XA Expired - Fee Related CN103808941B (en) | 2014-01-28 | 2014-01-28 | A kind of multifunctional protein reaction experiment device |
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| Country | Link |
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| CN (1) | CN103808941B (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201569655U (en) * | 2009-12-17 | 2010-09-01 | 东莞市兴仁保健食品有限公司 | Immunoblot antibody incubation box |
| CN201583540U (en) * | 2010-01-08 | 2010-09-15 | 陈锦 | Blotting incubation box |
| CN202305534U (en) * | 2011-11-14 | 2012-07-04 | 北京康为世纪生物科技有限公司 | Western blotting antibody incubation box |
| US8470541B1 (en) * | 2008-09-27 | 2013-06-25 | Boston Heart Diagnostics Corporation | Methods for separation and immuno-detection of biomolecules, and apparatus related thereto |
-
2014
- 2014-01-28 CN CN201410041596.XA patent/CN103808941B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8470541B1 (en) * | 2008-09-27 | 2013-06-25 | Boston Heart Diagnostics Corporation | Methods for separation and immuno-detection of biomolecules, and apparatus related thereto |
| CN201569655U (en) * | 2009-12-17 | 2010-09-01 | 东莞市兴仁保健食品有限公司 | Immunoblot antibody incubation box |
| CN201583540U (en) * | 2010-01-08 | 2010-09-15 | 陈锦 | Blotting incubation box |
| CN202305534U (en) * | 2011-11-14 | 2012-07-04 | 北京康为世纪生物科技有限公司 | Western blotting antibody incubation box |
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| Publication number | Publication date |
|---|---|
| CN103808941A (en) | 2014-05-21 |
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