CN204666632U - Immunohistochemical staining device - Google Patents
Immunohistochemical staining device Download PDFInfo
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- CN204666632U CN204666632U CN201520414705.8U CN201520414705U CN204666632U CN 204666632 U CN204666632 U CN 204666632U CN 201520414705 U CN201520414705 U CN 201520414705U CN 204666632 U CN204666632 U CN 204666632U
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- groove
- immunohistochemical staining
- staining device
- histotomy
- lower plate
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Abstract
The utility model discloses a kind of immunohistochemical staining device, comprise upper plate and lower plate, described upper plate is provided with multiple through hole, described lower plate is provided with multiple with through hole groove one to one, described via bottoms is sealed by biological membrane, and described biological membrane is raised to downwards in groove.When using the immunohistochemical staining device of this structure to carry out the dyeing of drift sheet to histotomy, cause histotomy to damage when picking histotomy can not only be avoided to float sheet dyeing, and the waste of reagent can be avoided.
Description
Technical field
The utility model relates to medical biotechnology experimental provision, is specifically related to a kind of immunohistochemical staining device.
Background technology
In medical experiment, utilizing the principle of antigen and antibody specific binding, adopt different antibodies to carry out immunochemistry dyeing to tissue, is detect one of body method that changes of function is the most frequently used under physiology or pathological state.In the immunostaining process of histotomy, paster method and drift sheet method is usually adopted to dye to it.Adopt paster method dyeing to be first, after paraffin organization or frozen tissue being cut into slices, be pasted on microslide, then detect according to the correlation step of immunohistochemical staining.Usually there is following problem in this detection method at present: 1, repeatedly rinse tissue in testing process, easily cause histotomy to come off; 2, because antibody is general costly, primary antibodie is therefore added and two anti-amounts are limited.Add in the operating process of antibody in experiment, because microslide or the slight inclination of tissue surface all may cause antibody effectively and contact tissue, affect the specific binding of antibody and antigen, thus to cause the failure of experiment detection.3, at present after tissue staining terminates, usual employing neutral resins, paraffin oil or glycerine carry out mounting to tissue, to preserve the positive findings of experiment, especially the preservation of fluorescent positive result, usually all adopt glycerine to carry out mounting to tissue, but such mountant easily cause cover glass displacement and can not well fixing organization and preserve coloration result.Float sheet method dyeing be then histotomy is positioned in a displacement apparatus complete dyeing institute in steps.This colouring method can avoid histotomy to come off and antibody and the problem such as tissue effectively contacts.But undertaken in immunohistochemistry/fluorescent dye process by drift sheet method at present, need by histotomy rinsing in different liquid repeatedly, and the article such as main needle point shift histotomy in rinse cycle, because histotomy thickness is thin, many at about 20-50 μm, therefore this process easily causes the damage of histotomy.And to carry out the dyeing of drift sheet be at present experimenter according to experience to be placed on reagent higher for the prices such as antibody voluntarily in different vessels and to dye, and without the experimental ware of comparatively seeking unity of standard, the experimenter lacked experience easily is caused to the waste of valuable reagent.
Therefore, be necessary for a kind of experimental apparatus being applicable to float sheet dyeing of above-mentioned two shortcomings design.
Utility model content
In view of this, the purpose of this utility model is to provide a kind of immunohistochemical staining device being applicable to float sheet dyeing, in dyeing course, can not only avoid causing histotomy to damage, and can avoid the waste of reagent.
The utility model is solved the problem by following technological means: a kind of immunohistochemical staining device, comprise upper plate and lower plate, described upper plate is provided with multiple through hole, described lower plate is provided with multiple with through hole groove one to one, described via bottoms is sealed by biological membrane, and described biological membrane is raised to downwards in groove.
Further, described upper sheet top surface is provided with label near through hole.
Further, described bottom portion of groove is provided with coating.
Further, described recess edge is provided with O-ring seal.
Further, main airway is provided with in described lower plate, described main airway is communicated with groove by branch airway, main airway is in communication with the outside by exhaust duct, described branch airway and groove junction are provided with by waterproof and the vibrating membrane made of air-locked material, also comprise the inflatable structure be communicated with main airway entrance, described inflatable structure comprises air bag and the airsac tube for communicated air bags and main airway entrance.
The beneficial effects of the utility model: immunohistochemical staining device of the present utility model, comprise upper plate and lower plate, described upper plate is provided with multiple through hole, described lower plate is provided with multiple with through hole groove one to one, described via bottoms is sealed by biological membrane, and described biological membrane is raised to downwards in groove.When using the immunohistochemical staining device of this structure to carry out the dyeing of drift sheet to histotomy, cause histotomy to damage when picking histotomy can not only be avoided to float sheet dyeing, and the waste of reagent can be avoided.
Accompanying drawing explanation
Below in conjunction with drawings and Examples, the utility model is further described.
Fig. 1 is structural representation of the present utility model.
Embodiment
Below with reference to accompanying drawing, the utility model is described in detail, as shown in Figure 1: a kind of immunohistochemical staining device, comprise upper plate 1 and lower plate 2, described upper plate is provided with multiple through hole 3, described lower plate is provided with multiple with through hole groove 4 one to one, described via bottoms is sealed by biological membrane 5, and described biological membrane is raised to downwards in groove.In dyeing course, upper plate and lower plate overlap, histotomy is positioned in through hole, the damping fluid of rinsing tissue then drips in the groove of correspondence, damping fluid infiltrate biological membrane can carry out rinsing to histotomy, when needing exchange buffering liquid, removing upper plate, changing the damping fluid in respective slot.In whole rinse cycle, histotomy or float in damping fluid, sink on biological membrane, do not need shift by needle point or provoke histotomy, therefore can not cause the damage of histotomy, in addition, the regular size of groove is certain, damping fluid adds in a groove, belongs to quantitative interpolation, therefore can not cause the waste of reagent.In addition, can arrange bound edge in the top edge of lower plate, when upper plate and lower plate overlap, bound edge just encases upper plate, plays the role of positioning to upper plate, makes through hole and the alignment of corresponding groove.
As the further improvement to technique scheme, described upper sheet top surface is provided with label 6 near through hole.The histotomy of which kind of classification that what label was used for showing inserting in through hole corresponding with it is.
As the further improvement to technique scheme, described bottom portion of groove is provided with coating 7, coating painted black, forms black background, is convenient to the staining conditions of tissues observed section.
As the further improvement to technique scheme, described recess edge is provided with O-ring seal 8, and O-ring seal seals, and prevents in dyeing course, damping fluid from groove and and through hole between gap location reveal.
As the further improvement to technique scheme, main airway 9 is provided with in described lower plate, described main airway is communicated with groove by branch airway 10, main airway is in communication with the outside by exhaust duct 11, described branch airway and groove junction are provided with by waterproof and the vibrating membrane 12 made of air-locked material, vibrating membrane can be plastic sheeting, also comprises the inflatable structure be communicated with main airway entrance, and described inflatable structure comprises air bag 13 and the airsac tube 14 for communicated air bags and main airway entrance.Carrying out in dyeing course to histotomy, extruding gasbag, interval is toward main airway inflation, and air-flow can make vibrating membrane heave and vibrate, thus causes damping fluid to ripple or fluctuate, and the damping fluid of fluctuation can improve the efficiency of histotomy being carried out to rinsing dyeing.
What finally illustrate is, above embodiment is only in order to illustrate the technical solution of the utility model and unrestricted, although be described in detail the utility model with reference to preferred embodiment, those of ordinary skill in the art is to be understood that, can modify to the technical solution of the utility model or equivalent replacement, and not departing from aim and the scope of technical solutions of the utility model, it all should be encompassed in the middle of right of the present utility model.
Claims (5)
1. an immunohistochemical staining device, it is characterized in that: comprise upper plate (1) and lower plate (2), described upper plate is provided with multiple through hole (3), described lower plate is provided with multiple with through hole groove (4) one to one, described via bottoms is by biological membrane (5) sealing, and described biological membrane is raised to downwards in groove.
2. immunohistochemical staining device according to claim 1, is characterized in that: described upper sheet top surface is provided with label (6) near through hole.
3. immunohistochemical staining device according to claim 2, is characterized in that: described bottom portion of groove is provided with coating (7).
4. immunohistochemical staining device according to claim 3, is characterized in that: described recess edge is provided with O-ring seal (8).
5. the immunohistochemical staining device according to claim 1-4 any one, it is characterized in that: in described lower plate, be provided with main airway (9), described main airway is communicated with groove by branch airway (10), main airway is in communication with the outside by exhaust duct (11), described branch airway and groove junction are provided with by waterproof and the vibrating membrane (12) made of air-locked material, also comprise the inflatable structure be communicated with main airway entrance, described inflatable structure comprises air bag (13) and the airsac tube (14) for communicated air bags and main airway entrance.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520414705.8U CN204666632U (en) | 2015-06-16 | 2015-06-16 | Immunohistochemical staining device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520414705.8U CN204666632U (en) | 2015-06-16 | 2015-06-16 | Immunohistochemical staining device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN204666632U true CN204666632U (en) | 2015-09-23 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201520414705.8U Expired - Fee Related CN204666632U (en) | 2015-06-16 | 2015-06-16 | Immunohistochemical staining device |
Country Status (1)
| Country | Link |
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| CN (1) | CN204666632U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109622093A (en) * | 2018-11-01 | 2019-04-16 | 中国人民解放军第二军医大学第二附属医院 | Hospital ship pathology station |
-
2015
- 2015-06-16 CN CN201520414705.8U patent/CN204666632U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109622093A (en) * | 2018-11-01 | 2019-04-16 | 中国人民解放军第二军医大学第二附属医院 | Hospital ship pathology station |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150923 Termination date: 20160616 |