CN209051802U - A kind of new membrane washes box - Google Patents

Abstract

The utility model relates to a kind of new membranes to wash box, including box body, box body is divided into main box and secondary box, it is placed in main box and the separable no hamper of main box, no hamper lower end surface is equipped with circular protrusions, it is equipped with baffle between main box and secondary box, is connected separately with the main box cover with locking plate and the secondary box cover with locking plate on main box and secondary box.Different size of no hamper can be placed according to film size, replacement is convenient, effectively improves and washes membrane efficiency；Secondary box is for placing the small article washed and used in membrane process, tweezers or marking pen etc..

Description

A kind of new membrane washes box

Technical field

The utility model relates to a kind of films to wash box, and especially a kind of film washes box, belong to molecular biology experiment technical field.

Background technique

In molecular biology, biochemistry and immunogenetics research, immunoblotting is common experimental method. The basic principle is that being coloured by specific antibody to the processed cell of gel electrophoresis or biological tissue samples.By dividing The position for analysing coloring obtains the information of specific protein expression in the cell or tissue analyzed with color depth.Albumen For matter blotting method using polyacrylamide gel electrophoresis, detected material is protein, and " probe " is antibody, and " colour developing " uses mark The secondary antibody of note.Using on solid phase carrier protein or polypeptide as antigen, play immune response with corresponding antibody, then with or same position The secondary antibody of element label reacts, and the specific target gene of electrophoretic separation is detected by substrate colour developing or autoradiograph The protein ingredient of expression, the technology are also widely used in detecting the expression of protein level.

The one kind of immunoblotting as most common molecular biology experiment, experimental procedure is very more, each step Suddenly the influence of experimental result is crucial, washing film is a critical process in immunoblotting experiment, washes film Quality directly affects whether exposed band is clear, and whether background is clean, when being cleaned with membrane regeneration liquor, if on film Antibody can not clean up, then the background that will cause film is too deep, cause protein band expression quantity not clear enough, so that result is not It can use.

So far, use round culture dish either of different sizes when washing film during laboratory practices mostly Box inside carry out；More more convenient was being operated with the box for putting 4 or 5 films of a specification of the same race Cheng Zhong, people are frequently encountered following problems: 1) each culture dish either box can only wash a film, while cannot be convenient Clean more than 10 films；2) culture dish leakproofness is poor, is easy to shake out by internal liquid when adjusting shaking speed；3) single or Person is that the identical box length and width of several specifications of disjunctor are relatively more fixed, and the size of film and the size of box cannot well complete Match, therefore we will use more film washing liquids, antibody and membrane regeneration liquor etc. could abundant cover film；4) cellulose nitrate film Surface is smooth, and usually round culture dish used or common cartridge bottom nor ordinary light are sliding, and film is easy to glue when leading to wash film In its inner wall, lead to wash that film is not clean, the item exposed has miscellaneous band, influences experimental result；5) washing clip band in membrane process is made The position that tweezers are not fixed often arbitrarily is placed in shaking table or experimental implementation, tweezers is caused often to be lost.Cause This, urgent need can improve traditional experiment room Membrane cleaning technology on the market, and Lai Tigao conventional efficient is reduced and put into not in experiment Necessary cost loss.

Utility model content

The technical problem to be solved by the present invention is to provide a kind of new membranes to wash box, can clean multiple films simultaneously, and Film suitable for sizes.

In order to solve the above problem, the specific technical solution of the utility model is as follows: a kind of new membrane washes box, including box body, Box body is divided into main box and secondary box, is placed in main box and is equipped with circular protrusions with the separable no hamper of main box, no hamper lower end surface, It is equipped with baffle between main box and secondary box, is connected separately with the main box cover with locking plate and the secondary box with locking plate on main box and secondary box Lid.Different size of no hamper can be placed according to immunoblotting film size, replacement is convenient, effectively improves and washes membrane efficiency； Secondary box is for placing the small article washed and used in membrane process, tweezers or marking pen etc..

The main box is equipped with the lock cooperated with locking plate, and the secondary box is equipped with the lock cooperated with locking plate.

Several are placed with without hamper in the main box.It can be selected according to the size and quantity of cleaning film without hamper Size and number.

The utility model bring has the beneficial effect that film washes that box structure is simple, low in cost, practical value is high, can be simultaneously Cleaning for multiple films of different sizes.

The utility model immunoblotting film washes box structure design rationally, and good appearance is easy to use, and practical value Height, it is most important to select most suitable film to wash box according to the specification of film, save the use of film washing liquid and membrane regeneration liquor, the use of antibody Number can greatly increase, and achieve the effect that sufficiently be incubated for immunoblotting film a small amount of antibody, reality is greatly saved The cost tested also can greatly accelerate conventional efficient.

Detailed description of the invention

Fig. 1 is that novel western film washes box structure figure.

Fig. 2 is uncovered box structure figure.

Fig. 3 is that western film washes structure chart after the main box cover of box and secondary box cover fastening.

Wherein, 1- box body, the main box of 1-1-, the main box cover of 1-11-, 1-12- latch I, 1-2- pair box, 1-21- pair box cover, 1- 22- latches II, and 2- is without hamper, 3- baffle.

Specific embodiment

As shown in Figure 1, a kind of new membrane washes box, including box body 1, box body 1 divides for main box 1-1 and secondary box 1-2, main box 1-1 Inside be placed with and be equipped with circular protrusions with the separable no hamper 2 of main box 1-1, no 2 lower end surface of hamper, main box 1-1 and secondary box 1-2 it Between be equipped with baffle 3, be connected separately with the main box cover 1-11 with locking plate and the secondary box with locking plate on main box 1-1 and secondary box 1-2 Cover 1-21；It is different and alternatively without hamper 2 that size shape can be placed in main box 1-1 box body, built-in no hamper 2 is placed with very Various ways can adjust at any time according to the size of film, can clean multiple films simultaneously, save very large space, while also be real It tests and brings great convenience.

The main box 1-1 is equipped with the lock 1-12 cooperated with locking plate, and the secondary box 1-2 is equipped with to be cooperated with locking plate Lock 1-22.

Several are placed with without hamper 2 in the main box 1-1.The length and width selection of built-in no hamper 2 is according to testing Length and width most suitable needed for film in journey, Gao Yuzhu box 1-1 box body are contour.

Embodiment 1

When needing to clean the film that do not cut off, according to the length and width of film, 3 a length of 5 cm may be selected, width is the uncovered of 9 cm Box 2 is built in main box, can clean 3 films that do not cut off simultaneously；For example it needs to clean the film cut off and do not cut off simultaneously When, Rational choice combination is built-in without hamper 2 according to the actual situation, needed for satisfaction.

Embodiment 2

After cutting off film according to molecular weight of albumen, according to the length and width of film, suitably without hamper 2, such as needed built in main box When cleaning 3 films cut off from centre simultaneously, then built-in 6 a length of 2.5 cm of main box, width is 9 cm without hamper, can 6 films symmetrically cut off are cleaned simultaneously；It is optional or on every film while when being cut into 4 part from centre there are two types of sample needs Select built in main box 12 2.5 × 4.5 cm without hamper, the combination of built-in no hamper 2 there are many mode, can according to it is required simultaneously More than 10 films of different sizes are cleaned, it is most suitable according to required selection.

Above-described is only the preferred embodiment of the utility model.It should be pointed out that for the ordinary skill people of this field For member, without departing from the principle of this utility model, several variations and modifications can also be made, also should be regarded as belonging to this The protection scope of utility model.

Claims (3)

1. a kind of new membrane washes box, including box body (1), it is characterised in that: box body (1) is divided into main box (1-1) and secondary box (1-2), It is placed in main box (1-1) and is equipped with circular protrusions, master with the separable no hamper (2) of main box (1-1), no hamper (2) lower end surface Baffle (3) are equipped between box (1-1) and secondary box (1-2), are connected separately on main box (1-1) and secondary box (1-2) with locking plate Main box cover (1-11) and secondary box cover (1-21) with locking plate.

2. a kind of new membrane as described in claim 1 washes box, it is characterised in that: the main box (1-1) is equipped with and locking plate The lock (1-12) of cooperation, the secondary box (1-2) are equipped with the lock (1-22) cooperated with locking plate.

3. a kind of new membrane as described in claim 1 washes box, it is characterised in that: be placed in the main box (1-1) several A no hamper (2).