CN207051297U

CN207051297U - A kind of convex bubble plate of immune detection

Info

Publication number: CN207051297U
Application number: CN201720611219.4U
Authority: CN
Inventors: 曹禹; 于淼; 李春辉; 韩宏光; 王仕祺
Original assignee: Individual
Current assignee: Individual
Priority date: 2017-05-27
Filing date: 2017-05-27
Publication date: 2018-02-27
Anticipated expiration: 2027-05-27

Abstract

The utility model discloses a kind of convex bubble plate of immune detection, including plate and convex bubble, the convex bubble is raised from the plate, and junction is round mouth, and antigen or antibody are coated with the outside of the convex bubble.Antigen or antibody are coated with the outside of convex bubble, the transfer of solid phase carrier is realized, colorimetric detection can be carried out, make immune detection more flexible and convenient.

Description

A kind of convex bubble plate of immune detection

Technical field

It the utility model is related to a kind of convex bubble plate of immune detection.

Background technology

Immunoassay technology refers to utilize the specific principle of immune response, establishes the technologies of various detections and analysis.Often Rule immunoassay technology is mainly used for detecting the ion vitro immunization serological reaction or immunoserology technology of antigen or antibody.Exempt from Epidemic disease serological technique is built upon the detection technique on the basis of antigen and antibody specific reaction.The development of modern immunoassay technology Very fast, existing many new methods and techniques occur.Due to antigen（Or antibody）Binding label it is different, divide enzyme-linked exempt from again The immunization experiment type such as epidemic disease, electrochemiluminescent immunoassay, fluorescence immunoassay.No matter using which kind of labelling technique, wherein all referring to antigen-antibody Pre-coated, i.e., the process being affixed to antigen or antibody on solid phase carrier, the form of solid phase carrier has small test tube and micro- Quantitative response plate, it is the container that can also double as reaction the characteristics of small test tube, is finally putting into colorimetric in spectrophotometer.The most frequently used load Body is micro-reaction plate, and the product for being exclusively used in ELISA measure is also referred to as elisa plate, and international standard plate shape is 8 × 12 96 cellular types, for ease of making the detection of a small amount of sample, have and 8 or 12 hole bars, after being put into mounting, size and standard are made Elisa plate is identical.The characteristics of elisa plate is the detection that can carry out a large amount of samples simultaneously, and can be fast on specific colorimeter Fast reading goes out result.Existing a variety of self-reacting devices are used for the ELISA detections of micro-reaction template now, including are loaded, wash, protect The steps such as temperature, colorimetric.But these solid phase carriers are all fixed containers, generally it is also responsible for holding the duty of reaction reagent in an experiment Can, it is impossible to realize the transfer of solid phase carrier, limit the flexibility of experiment.

Utility model content

In order to overcome the above-mentioned deficiencies of the prior art, the purpose of this utility model is to provide a kind of convex bubble plate of immune detection, Antigen or antibody are coated with the outside of convex bubble, the transfer of solid phase carrier is realized, colorimetric detection can be carried out, make immune detection more Flexible and convenient.To achieve these goals, the utility model adopts the following technical scheme that：

A kind of convex bubble plate of immune detection, including plate and convex bubble, the convex bubble is raised from the plate, and junction is circle Mouthful, it is coated with antigen or antibody on the outside of the convex bubble.

Further, the quantity for the convex bubble being set on each plate is 1 to 3200, preferably：16 to 320.

Further, the plate in the side with the round mouth with air-tight membrane.

Further, the quantity for the convex bubble being set on each plate is 96, the matrix that the convex bubble is 12 × 8.

Further, a diameter of the 0.1 to 1cm of the round mouth；It is preferred that：0.5 to 0.8cm；More preferably：0.64cm.

Further, the convex bubble is shallow bubble, hemisphere bubble, post ball steep or cone bubble.

Further, the convex bubble sheet material matter is polystyrene.

Region bovine serum albumin(BSA) outside the convex bubble（Or other do not possess the material of reactivity）Coating, is avoided Interference.

The convex bubble is shallow bubble, hemisphere bubble, post ball bubble or cone four types of bubble, the hemisphere bubble refer to being shaped as convex bubble Hemisphere face, the shallow bubble refer to the sphere being shaped as less than hemisphere of convex bubble, and the post ball bubble refers to cylindric at round mouth prolong Stretch and sphere is connected in bottom, the cylindric extended distance is less than or equal to the diameter of the round mouth, and the cone bubble refers to convex bubble Be shaped as the conical surface of the round mouth as bottom surface.The convex bubble of four types is with parallel to obtained by the convex bubble of the arbitrary plane of round mouth interception Circle is no more than round mouth.

The convex bubble plate is plastic material, such as polystyrene, and polystyrene has the performance of stronger adsorbed proteins, resists Body or proteantigen absorption retain original immunocompetence afterwards thereon, can be made into various forms, in continuous mode, its conduct Carrier and container, it is not involved in chemically reacting, its is cheap in addition, so being generally used.

The beneficial effects of the utility model：

1. being coated with antigen or antibody on the outside of convex bubble, the transfer of solid phase carrier is realized, can be developed the color or colorimetric is examined Survey, make immune detection more flexible and convenient.Small test tube and micro-reaction plate are reacted on the inside of cavity, and this practicality New is that antigen or antibody are coated with the outside of convex bubble, is reacted on the outside of convex bubble, and corresponding operation is changed to convex bubble plate Convex bubble face is put on the liquid such as testing sample, colour reagent, cleaning reagent, eliminates the frequently sample-adding operation per hole, while Ensure each convex bubble identical reaction condition, make detection more flexible and convenient.

2. convex bubble can be arranged to different sizes, different shapes by different according to specific testing goal.

3. convex bubble plate convex bubble face is put in testing sample, colour reagent, on cleaning reagent when convex bubble plate swim on liquid level, Negative effect that is easy to operate, avoiding the evaporator strip of small volume reaction reagent liquid from.

4. the translucency between convex bubble is homogeneous, ensure the comparability of colorimetric detection between convex bubble.

5. adding solution within convex bubble, convex effect of the bubble with certain convex mirror, facilitate the observation of result of the test.

6. film is air-tightness, film is opened before development step, prevents reaction solution of previous experiments step etc. from entering Enter into convex bubble to influence colour developing result.

7. different antigen or antibody can be incubated on the different convex bubbles of same convex bubble plate, a variety of biological substances are realized Detection.

8. the reagents such as testing sample, colour reagent, cleaning reagent may be reused, be advantageous to save.

9. the preparation technology of convex bubble plate is simple, cost is low, beneficial to commercial introduction.

Brief description of the drawings

Fig. 1 is the utility model structure diagram；

Fig. 2 is different types of convex bubble structure schematic diagram；

Fig. 3 is membrane structure schematic diagram.

Wherein, 1. plate, 2. convex bubbles, 3. round mouths, 4. films, 21. shallow bubbles, 22. hemisphere bubble, 23. post balls bubble, 24. cone bubbles.

Embodiment

The utility model is described in further detail below in conjunction with the accompanying drawings.

Embodiment 1：

As shown in figure 1, a kind of convex bubble plate of immune detection, including plate 1 and convex bubble 2, the convex bubble 2 is raised from the plate 1, And junction is round mouth 3, the convex outside of bubble 2 is coated with antigen or antibody.

Embodiment 2：

As shown in figure 1, a kind of convex bubble plate of immune detection, including plate 1 and convex bubble 2, the convex bubble 2 is raised from the plate 1, And junction is round mouth 3, the convex outside of bubble 2 is coated with antigen or antibody.The number of the convex bubble 2 is set on each plate 1 Measure as 1 to 3200, preferably：16 to 320.

Embodiment 3：

As shown in Figure 1,3, on the basis of embodiment 1 or 2, the plate 1 is in the side with the round mouth 3 with airtight Property film 4.The air-tight membrane 4 seals for 1 one weeks along the plate.

Embodiment 4：

As shown in Figure 1,3, on the basis of embodiment 1 or 2 or 3, the quantity of the convex bubble 2 is set on each plate 1 For 96, matrix that the convex bubble 2 is 12 × 8.

Embodiment 5：

As shown in Figure 1,3, on the basis of embodiment 1 or 2 or 3 or 4, a diameter of the 0.1 to 1cm of the round mouth 3；It is excellent Choosing：0.5 to 0.8cm；More preferably：0.64cm.

Embodiment 6：

As shown in Figure 1,3, on the basis of embodiment 1 or 2 or 3 or 4 or 5, it is described it is convex bubble 2 for it is shallow bubble 21, hemisphere bubble 22, Post ball bubble 23 or cone bubble 24.

Embodiment 7：

As shown in Figure 1,3, on the basis of embodiment 1 or 2 or 3 or 4 or 5 or 6, the convex bubble sheet material matter is polyphenyl second Alkene.

It is not new to this practicality although above-mentioned specific embodiment of the present utility model is described with reference to accompanying drawing The limitation of type protection domain, one of ordinary skill in the art should be understood that on the basis of the technical solution of the utility model, ability Field technique personnel need not pay the various modifications that creative work can make or deformation still in protection model of the present utility model Within enclosing.

Claims

1. a kind of convex bubble plate of immune detection, including plate（1）With convex bubble（2）, the convex bubble（2）From the plate（1）Upper projection, and even It is round mouth to meet place（3）, the convex bubble（2）Outside is coated with antigen or antibody.

2. convex bubble plate as claimed in claim 1, it is characterized in that, each plate（1）It is upper that the convex bubble is set（2）Quantity be 1 to 3200.

3. convex bubble plate as claimed in claim 1, it is characterized in that, the plate（1）With the round mouth（3）Side there is gas Close property film（4）.

4. convex bubble plate as claimed in claim 1, it is characterized in that, each plate（1）It is upper that the convex bubble is set（2）Quantity be 96, the convex bubble（2）For 12 × 8 matrix.

5. convex bubble plate as claimed in claim 1, it is characterized in that, the round mouth（3）A diameter of 0.1 to 1cm.

6. convex bubble plate as claimed in claim 1, it is characterized in that, the round mouth（3）A diameter of 0.5 to 0.8cm.

7. convex bubble plate as claimed in claim 1, it is characterized in that, the round mouth（3）A diameter of 0.64cm.

8. convex bubble plate as claimed in claim 1, it is characterized in that, the convex bubble（2）For shallow bubble（21）, hemisphere bubble（22）, post ball Bubble（23）Or cone bubble（24）.

9. convex bubble plate as claimed in claim 1, it is characterized in that, its material is polystyrene.