WO2011000190A1 - Long-term portable kit for rapid testing of sulfhydryl in body fluid - Google Patents
Long-term portable kit for rapid testing of sulfhydryl in body fluid Download PDFInfo
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- WO2011000190A1 WO2011000190A1 PCT/CN2009/075425 CN2009075425W WO2011000190A1 WO 2011000190 A1 WO2011000190 A1 WO 2011000190A1 CN 2009075425 W CN2009075425 W CN 2009075425W WO 2011000190 A1 WO2011000190 A1 WO 2011000190A1
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- reagent
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- sulfhydryl
- thiol
- based developer
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
Definitions
- the utility model relates to a kit, in particular to a kit for long-acting and rapid determination of sulfhydryl groups in body fluids.
- Lin Genchun In 1986, the inventor of this application, Lin Genchun, successfully designed the detection method of thiol in body fluids such as urine, gastric juice and ascites for the first time, and published related papers and academic reports (Lin Genchun. Determination of urinary sputum in the diagnosis of malignant tumors. Fujian Medical Journal of the Academy of Sciences 1986; 20 ( 2 ) : 39 ).
- this method requires the reagent to be ready to use, and the prepared thiol-based detection reagent and the body fluid to be tested must be simultaneously added to the test tube for simultaneous detection of the sulfhydryl group of the body fluid; and an analytical balance, a biochemical analyzer or a spectrophotometer must be provided, Precision instruments such as centrifuges; the entire inspection process can be completed by a professional technician in a professional laboratory for 1 to 1.5 hours.
- This method has been used for more than 20 years and has played an important role in the detection of sputum.
- time, space, speed of inspection, work efficiency, and inspection cost are all limited, which cannot meet the needs of large-scale and large-scale use. Summary of the invention
- the object of the present invention is to provide a long-acting, fast, and highly efficient portable long-acting rapid body fluid thiol assay kit.
- the present invention adopts the following design scheme:
- a portable long-acting rapid body fluid thiol assay kit comprising an outer package having at least one test plate and at least one set of sealed reagent droppers and a set of sealed, separated thiol-based developer carrier films; a set of sealed reagent dropper at least two, one of which is a reagent dropper without a sulfhydryl complexing agent, and the other is a reagent dropper containing a sulfhydryl complexing agent; the set of sulfhydryl chromogen carrier film, At least two are adsorbed with an equal amount of sulfhydryl-based developer; the test panel is provided with a test zone, and the number of test zones is the same as that of the thiol-based developer carrier film, at least two.
- the set of sulfhydryl-based developer carrier films are placed apart from one another and have a sealed package structure.
- the utility model of the portable long-acting rapid body fluid thiol determination kit can be extended to four years, the measurement speed can be accelerated by 30 to 45 times, and the cost is reduced by 33 times.
- the measurement can be carried out in an ordinary room, without the need to analyze balances, biochemical analyzers or spectrophotometers, centrifuges and other equipment, non-color, color-blind professionals and non-professionals can use, can make China's body fluid sputum popular detection rate exceeds 90%.
- the utility model has the advantages that: the reagent dropper and the thiol-based developer carrier film are packaged together with the test plate in the kit, which is convenient to carry around; and is not required to be measured in a specific laboratory and related equipment environment, Most people can use it, suitable for large-scale and large-scale popular applications.
- FIG. 1 is a schematic structural view of a preferred embodiment of the present invention (top view, without a cover)
- the cartridge of the portable long-acting rapid body fluid thiol assay kit of the present invention has a test plate, a reagent dropper and a thiol-based developer carrier film.
- the reagent cartridge 1 has a test plate 2 and two reagent droppers, and the two reagent droppers are a reagent dropper (R1) No. 5 and a reagent dropper (R2) No. 2 8 .
- the test board 2 On the test board 2, a T1 test area 3 and a T2 test area 4 are separately disposed; in each of the test areas 3 and the test area 4, an S1 ⁇ -based developer carrier film 6 and an S2 ⁇ -based developer carrier film 7 are respectively disposed.
- S1 ⁇ -based developer carrier film 6 and S2 ⁇ -based developer carrier film 7 adsorb an equal amount of sulfhydryl-based color developer, and the two sulfhydryl-based developer carrier films are separated from each other, and should be sealed and stored in a separate package before use.
- 1 is a structure of a preferred embodiment of the portable long-acting rapid body fluid thiol assay kit of the present invention, which is a single-use kit.
- the test plate, the reagent dropper, the thiol-based developer carrier film, that is, the box, may be placed in a kit twice (or for two people at the same time) or multiple times (or for multiple persons at the same time).
- the two sets of sealed ruthenium-based developer carrier membranes of the configuration should be adsorbed with the same amount of ruthenium-based developer as two or more, and the products should be kept in a sealed and sealed state during storage and use.
- the measurement of the portable long-acting fast body fluid thiol determination kit of the utility model is achieved by: firstly placing the test plate on the table surface; unsealing the sealed S 1 and S 2 ruthenium-based developer carrier film, and coloring the S 1 thiol group
- the carrier carrier film is placed flat on the test plate T1 test area, and the S2 thiol-based developer carrier film is placed flat on the test plate T2 test area; the R1 reagent dropper and the R2 reagent dropper are sequentially opened, and the reagent in the R1 reagent dropper is dropped into the S1 In the center of the thiol-based developer carrier film, the reagent in the R2 reagent dropper is dropped into the center of the S2 thiol-based developer carrier film, and the amounts of the R1 and R2 reagents respectively dropped into the S 1 and S 2 thiol-based developer carrier membranes are equal; The body fluid of the sulfhydryl group was added to the center
Abstract
A long-term portable kit for rapid testing of sulfhydryl in body fluid is provided. The kit includes a package; at least one test plate, a set of sealed reagent droppers and a set of sealed films with developing reagent for SH in the package. One set of sealed reagent droppers comprises at least two reagent droppers. One reagent dropper doesn’t contain sulfhydryl complexing agent, while the other reagent dropper contains sulfhydryl complexing agent. One set of sealed films with developing reagent for SH comprises at least two films which are separated, and adsorb the same amount of developing reagent respectively. At least two testing regions are disposed in the test plate to support the films with developing reagent for SH, whose amount is the same as that of the films with developing reagent for SH.
Description
便携式长效快速体液巯基测定试剂盒 技术领域 Portable long-acting fast body fluid thiol determination kit
本实用新型涉及一种试剂盒，尤其涉及一种便携式长效而快速测定体液中 巯基的试剂盒。 背景技术 The utility model relates to a kit, in particular to a kit for long-acting and rapid determination of sulfhydryl groups in body fluids. Background technique
1986年，本申请的发明人林根春首次成功设计出尿液、 胃液、 腹水等体液 中巯基的检测方法， 发表了相关论文和学术报告 （林根春。 测定尿中巯基物对 恶性肿瘤的诊断。 福建医学院学报 1986 ; 20 ( 2 ) ： 39 ) 。 然而， 这种方法要 求试剂即配即用， 所配制的巯基检测试剂和待测体液必须同时加入试管混合处 理， 即时检测该体液的巯基； 并且必须具备分析天平、 生化分析仪或分光光度 计、 离心机等精密仪器设备； 由专业技术人员在专业实验室操作 1〜1. 5 小时 才能完成整个检验过程。 该方法经历 20 多年， 在检测巯基工作中发挥了重要 作用。 但在时间、 空间、 检测速度、 工作效力、 检查成本都受到很大限制， 不 能满足大规模和大范围使用需要。 发明内容 In 1986, the inventor of this application, Lin Genchun, successfully designed the detection method of thiol in body fluids such as urine, gastric juice and ascites for the first time, and published related papers and academic reports (Lin Genchun. Determination of urinary sputum in the diagnosis of malignant tumors. Fujian Medical Journal of the Academy of Sciences 1986; 20 ( 2 ) : 39 ). However, this method requires the reagent to be ready to use, and the prepared thiol-based detection reagent and the body fluid to be tested must be simultaneously added to the test tube for simultaneous detection of the sulfhydryl group of the body fluid; and an analytical balance, a biochemical analyzer or a spectrophotometer must be provided, Precision instruments such as centrifuges; the entire inspection process can be completed by a professional technician in a professional laboratory for 1 to 1.5 hours. This method has been used for more than 20 years and has played an important role in the detection of sputum. However, time, space, speed of inspection, work efficiency, and inspection cost are all limited, which cannot meet the needs of large-scale and large-scale use. Summary of the invention
本实用新型的目的是提供一种长效、 快速、 高效率的便携式长效快速体液 巯基测定试剂盒。 The object of the present invention is to provide a long-acting, fast, and highly efficient portable long-acting rapid body fluid thiol assay kit.
为实现上述目的， 本实用新型采取以下设计方案： To achieve the above objectives, the present invention adopts the following design scheme:
一种便携式长效快速体液巯基测定试剂盒， 包括一个外包装盒， 盒内至少 有一测试板及至少一组密封的试剂滴管和一组密封、 分隔放置的巯基显色剂载 体膜； 所述一组密封的试剂滴管至少为 2支， 其一为不含巯基络合剂的试剂滴 管， 另一为含巯基络合剂的试剂滴管； 所述一组巯基显色剂载体膜， 至少为 2 个， 均吸附着等量的巯基显色剂； 所述一个测试板上设有测试区， 测试区的开 设数量与巯基显色剂载体膜数量相同， 至少为 2个。 所述的一组巯基显色剂载体膜相互分隔放置， 且带有密封包装结构。
本实用新型便携式长效快速体液巯基测定试剂盒有效使用期可延长至四 年， 测定速度可加快 30〜45倍， 成本降低 33倍。 测定可在普通室内进行， 无 需分析天平、 生化分析仪或分光光度计、 离心机等仪器设备， 无色弱、 色盲的 专业人员和非专业人员都能使用， 可使中国的体液巯基普及检测率超过 90%。 本实用新型的优点是： 试剂滴管及巯基显色剂载体膜与测试板一并包装在 试剂盒内， 便于随身携带； 且毋需在特定的实验室及具备相关设备的环境下进 行测定， 大多人员都能使用， 适宜大范围和大规模普及应用。 附图说明 A portable long-acting rapid body fluid thiol assay kit comprising an outer package having at least one test plate and at least one set of sealed reagent droppers and a set of sealed, separated thiol-based developer carrier films; a set of sealed reagent dropper at least two, one of which is a reagent dropper without a sulfhydryl complexing agent, and the other is a reagent dropper containing a sulfhydryl complexing agent; the set of sulfhydryl chromogen carrier film, At least two are adsorbed with an equal amount of sulfhydryl-based developer; the test panel is provided with a test zone, and the number of test zones is the same as that of the thiol-based developer carrier film, at least two. The set of sulfhydryl-based developer carrier films are placed apart from one another and have a sealed package structure. The utility model of the portable long-acting rapid body fluid thiol determination kit can be extended to four years, the measurement speed can be accelerated by 30 to 45 times, and the cost is reduced by 33 times. The measurement can be carried out in an ordinary room, without the need to analyze balances, biochemical analyzers or spectrophotometers, centrifuges and other equipment, non-color, color-blind professionals and non-professionals can use, can make China's body fluid sputum popular detection rate exceeds 90%. The utility model has the advantages that: the reagent dropper and the thiol-based developer carrier film are packaged together with the test plate in the kit, which is convenient to carry around; and is not required to be measured in a specific laboratory and related equipment environment, Most people can use it, suitable for large-scale and large-scale popular applications. DRAWINGS
图 1 为本实用新型一较佳实施例结构示意图 （俯视， 不带有盒盖） 具体实施方式 1 is a schematic structural view of a preferred embodiment of the present invention (top view, without a cover)
如图 1所示， 本实用新型便携式长效快速体液巯基测定试剂盒的盒内置有 测试板及试剂滴管和巯基显色剂载体膜。 As shown in Fig. 1, the cartridge of the portable long-acting rapid body fluid thiol assay kit of the present invention has a test plate, a reagent dropper and a thiol-based developer carrier film.
图 1所示实施例中， 其试剂盒 1内有一测试板 2和两支试剂滴管， 该两支 试剂滴管是 1号试剂滴管 （R1 ) 5和 2号试剂滴管 （R2 ) 8。 在测试板 2上分设 出 T1测试区 3、 T2测试区 4;在测试区 3和测试区 4的各辖区内分别置有 S1巯基 显色剂载体膜 6、 S2巯基显色剂载体膜 7。 In the embodiment shown in Fig. 1, the reagent cartridge 1 has a test plate 2 and two reagent droppers, and the two reagent droppers are a reagent dropper (R1) No. 5 and a reagent dropper (R2) No. 2 8 . On the test board 2, a T1 test area 3 and a T2 test area 4 are separately disposed; in each of the test areas 3 and the test area 4, an S1 巯-based developer carrier film 6 and an S2 巯-based developer carrier film 7 are respectively disposed.
上述的 S1巯基显色剂载体膜 6和 S2巯基显色剂载体膜 7吸附着等量巯基显 色剂， 且两个巯基显色剂载体膜相互分隔， 使用前应密封、 分隔包装保存。 图 1 是本实用新型便携式长效快速体液巯基测定试剂盒一较佳实施例结 构， 属一次性使用试剂盒。 亦可根据需要在一个试剂盒中置有两次 （或同时供 两人） 或多次 （或同时供多人） 测定用的测试板、 试剂滴管、 巯基显色剂载体 膜， 亦即盒内置有至少一个测试板及至少一组密封的试剂滴管和一组巯基显色 剂载体膜， 还可以是若干个测试板并配备若干密封的试剂滴管和巯基显色剂载 体膜， 以供多人测定或个人多次测定用； 此处不一一列举其组合实例。 所述配 置的一组密封的巯基显色剂载体膜无论是两个还是多个， 均应吸附着等量的巯 基显色剂， 产品封装及使用中的保存均应保持彼此分隔、 密闭状态。
上述各实施例可在不脱离本实用新型的范围下加以若干变化， 故以上的说 明所包含及附图中所示的结构应视为例示性， 而非用以限制在本实用新型的申 请专利范围。 The above-mentioned S1 巯-based developer carrier film 6 and S2 巯-based developer carrier film 7 adsorb an equal amount of sulfhydryl-based color developer, and the two sulfhydryl-based developer carrier films are separated from each other, and should be sealed and stored in a separate package before use. 1 is a structure of a preferred embodiment of the portable long-acting rapid body fluid thiol assay kit of the present invention, which is a single-use kit. The test plate, the reagent dropper, the thiol-based developer carrier film, that is, the box, may be placed in a kit twice (or for two people at the same time) or multiple times (or for multiple persons at the same time). Built-in at least one test plate and at least one set of sealed reagent dropper and a set of thiol-based developer carrier film, or a plurality of test plates and equipped with a plurality of sealed reagent droppers and thiol-based developer carrier films for Multi-person measurement or personal measurement for multiple times; examples of combinations thereof are not listed here. The two sets of sealed ruthenium-based developer carrier membranes of the configuration should be adsorbed with the same amount of ruthenium-based developer as two or more, and the products should be kept in a sealed and sealed state during storage and use. The above embodiments may be modified without departing from the scope of the invention, and the structures included in the above description and the drawings are to be considered as illustrative and not intended to limit the invention. range.
本实用新型便携式长效快速体液巯基测定试剂盒的测定是这样实现的： 首 先将测试板平放在台面上； 拆封密封的 S 1和 S2巯基显色剂载体膜， 将 S 1巯 基显色剂载体膜平放在测试板 T1测试区， S2巯基显色剂载体膜平放在测试板 T2测试区； 依次打开 R1试剂滴管和 R2试剂滴管， 将 R1试剂滴管内的试剂滴 入 S1巯基显色剂载体膜中央， 将 R2试剂滴管内的试剂滴入 S2巯基显色剂载 体膜中央， R1和 R2试剂分别滴入 S 1和 S2巯基显色剂载体膜的量相等； 把待 测巯基的体液等量地分别加入 S 1和 S2巯基显色剂载体膜中央； 观测 S 1和 S2 巯基显色剂载体膜的显色反应： 当 S 1巯基显色剂载体膜比 S2巯基显色剂载体 膜显色更深时， 结果为巯基测定阳性， 也可根据 S 1巯基显色剂载体膜比 S2巯 基显色剂载体膜显色更深的程度不同进行半定量记录和报告； 当 S 1和 S2巯基 显色剂载体膜的显色深浅度相同或二者都不显色时， 结果为巯基测定阴性。
The measurement of the portable long-acting fast body fluid thiol determination kit of the utility model is achieved by: firstly placing the test plate on the table surface; unsealing the sealed S 1 and S 2 ruthenium-based developer carrier film, and coloring the S 1 thiol group The carrier carrier film is placed flat on the test plate T1 test area, and the S2 thiol-based developer carrier film is placed flat on the test plate T2 test area; the R1 reagent dropper and the R2 reagent dropper are sequentially opened, and the reagent in the R1 reagent dropper is dropped into the S1 In the center of the thiol-based developer carrier film, the reagent in the R2 reagent dropper is dropped into the center of the S2 thiol-based developer carrier film, and the amounts of the R1 and R2 reagents respectively dropped into the S 1 and S 2 thiol-based developer carrier membranes are equal; The body fluid of the sulfhydryl group was added to the center of the S 1 and S 2 thiol-based developer carrier film in equal amounts; the color reaction of the S 1 and S 2 thiol-based developer carrier film was observed: when the S 1 巯-based developer carrier film was colored than the S 2 thiol group When the agent carrier film develops darker, the result is positive for the thiol determination, and may also be semi-quantitatively recorded and reported according to the degree of darkness of the S 1 巯-based developer carrier film than the S 2 巯-based developer carrier film; S2 base When the color development agent carrier film had the same color depth or neither of the color development, the result was a negative determination of the thiol group.
Claims
1、 一种便携式长效快速体液巯基测定试剂盒， 包括有试剂外包装盒， 其 特征在于： 盒内至少有一测试板及至少一组密封的试剂滴管和一组密封的巯基 显色剂载体膜； 所述一组密封的试剂滴管至少为 2支， 其一为不含巯基络合剂 的试剂滴管， 另一为含巯基络合剂的试剂滴管； 所述一组密封的巯基显色剂载 体膜至少 2个； 所述测试板上设有一组承载巯基显色剂载体膜的测试区， 测试 区的数量与密封的巯基显色剂载体膜数量相同， 至少为 2个。 A portable long-acting rapid body fluid thiol assay kit comprising a reagent outer package, characterized in that: at least one test plate and at least one set of sealed reagent dropper and a set of sealed thiol-based developer carriers are contained in the cartridge a set of sealed reagent dropper tubes of at least two, one of which is a reagent dropper containing no sulfhydryl complexing agent, and the other is a reagent dropper containing a sulfhydryl complexing agent; the set of sealed sulfhydryl groups The developer carrier film is at least two; the test plate is provided with a set of test zones carrying a ruthenium-based developer carrier film, and the number of test zones is the same as that of the sealed ruthenium-based developer carrier film, at least two.
2、 根据权利要求 1 所述的便携式长效快速体液巯基测定试剂盒， 其特征 在于： 所述一组密封的巯基显色剂载体膜均为吸附着等量巯基显色剂的载体 膜。 The portable long-acting rapid body fluid thiol assay kit according to claim 1, wherein: said set of sealed thiol-based developer carrier membranes are carrier membranes to which an equivalent amount of sulfhydryl-based developer is adsorbed.
3、 根据权利要求 1 所述的便携式长效快速体液巯基测定试剂盒， 其特征 在于： 所述的各巯基显色剂载体膜彼此分隔放置， 且带有密封包装结构。
3. The portable long-acting rapid body fluid thiol assay kit according to claim 1, wherein: each of the thiol-based developer carrier membranes are placed apart from each other and have a sealed package structure.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200920164393.4 | 2009-07-02 | ||
| CN2009201643934U CN201438190U (en) | 2009-07-02 | 2009-07-02 | Portable kit for long-time effective and fast detection of sulfhydryl in body fluids |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| WO2011000190A1 true WO2011000190A1 (en) | 2011-01-06 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/CN2009/075425 WO2011000190A1 (en) | 2009-07-02 | 2009-12-09 | Long-term portable kit for rapid testing of sulfhydryl in body fluid |
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| Country | Link |
|---|---|
| CN (1) | CN201438190U (en) |
| WO (1) | WO2011000190A1 (en) |
Cited By (1)
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| EP4241750A2 (en) | 2014-12-05 | 2023-09-13 | Egg Medical, Inc. | A multimodality medical procedure mattress-based device |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103424402B (en) * | 2013-07-02 | 2017-03-29 | 福建省明溪海天蓝波生物技术有限公司 | A kind of body fluid sulfydryl detection kit |
| CN104089953A (en) * | 2014-07-19 | 2014-10-08 | 青岛贝尔奥生物科技有限公司 | Method for rapidly determining sulfydryl in urine |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| EP4241750A2 (en) | 2014-12-05 | 2023-09-13 | Egg Medical, Inc. | A multimodality medical procedure mattress-based device |
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