CN210876440U - Manual plate washing device - Google Patents
Manual plate washing device Download PDFInfo
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- CN210876440U CN210876440U CN201921139588.3U CN201921139588U CN210876440U CN 210876440 U CN210876440 U CN 210876440U CN 201921139588 U CN201921139588 U CN 201921139588U CN 210876440 U CN210876440 U CN 210876440U
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- plate washing
- needle
- liquid storage
- storage bottle
- washing
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Abstract
The utility model discloses a manual plate washing device, which comprises a liquid storage bottle, a plate washing needle, a connecting pipe and a connecting cover; the detachable connecting cover is installed on the bottle mouth of the liquid storage bottle, the plate washing needle is installed on the connecting cover through the connecting pipe, the one-way water outlet valve is arranged on the connecting pipe, and the one-way air inlet valve is arranged at the lower end of the bottle body of the liquid storage bottle. The improved manual plate washing device provided by the utility model can save time and labor for washing operations in experiments such as enzyme-linked immunosorbent assay and effectively improve efficiency.
Description
Technical Field
The utility model relates to a biological experiment apparatus, in particular to a porous manual plate washing device for enzyme-linked immunosorbent assay.
Background
Enzyme-linked immunosorbent assay (ELISA) is a widely used technique in enzyme immunoassay, and its basic method is to coat known antigen or antibody on the surface of solid phase carrier (such as polystyrene micro reaction plate), after washing, add the sample to be tested, add substrate for color development, and after terminating the reaction, measure the absorbance (OD value). This technique can be used to: (1) localization of various intracellular components by immunoenzyme staining, (2) study of synthesis of anti-enzyme antibodies, (3) visualization of trace immunoprecipitation reaction, and (4) quantitative detection of antigen or antibody components in body fluid.
The washing step plays an important role during the ELISA experiments: after the antigen or the antibody is coated on the surface of the solid phase carrier, free components in the liquid phase need to be washed away through a washing step; after a sample to be detected is added, substances which are remained on the solid phase carrier and are not combined with the solid phase antigen or the antibody are removed through a washing step; in addition, since the adsorption of proteins by plastics such as polystyrene, which are commonly used as the surface of a solid phase carrier, is general, a washing step is required to remove interfering substances that are not specifically adsorbed to the solid phase carrier during the reaction.
At present, in a laboratory, the washing modes of an ELISA experiment generally comprise 2 types: and washing plates by a manual plate washing machine and a plate washing machine. The reaction plate is mostly washed by a plate washing machine in large hospitals, and the reaction plate is generally washed by hands in small and medium hospitals and research and development laboratories. The plate washing machine has the advantages of less plate washing human factors, high speed and constant conditions, but the equipment cost is high, so the plate washing machine is not as wide in application range as manual plate washing; in contrast, the plate washing by hand is lower in cost and easier to operate, and the common method is to use a laboratory bent-mouth liquid washing bottle to wash the plate, but the method is time-consuming and labor-consuming to operate and lower in efficiency, especially when the number of reaction plates to be washed is large; and the liquid in the bent nozzle of the washing liquid bottle is easy to flow back, so that the washing liquid in the bottle is polluted, a sample is polluted, and data is influenced.
SUMMERY OF THE UTILITY MODEL
The main object of the utility model is to provide a manual board device of washing of modified improves and washes board efficiency, and can prevent the pollution of washing liquid.
In order to achieve the purpose, the utility model discloses a manual plate washing device, which comprises a liquid storage bottle, a plate washing needle, a connecting pipe and a connecting cover; the plate washing needle is arranged above the connecting cover through the connecting pipe and forms a whole, and the whole is detachably arranged at the bottle mouth of the liquid storage bottle through the connecting cover; the connecting pipe is provided with a one-way water outlet valve; the lower end of the liquid storage bottle body is provided with a one-way air inlet valve.
The plate washing needles are arranged in a single row of a plurality of plate washing needle heads; specifically, the plate washing needles are provided with eight plate washing needle heads, each needle head is provided with an independent switch, and the corresponding switch can be turned on according to the required needle number to carry out plate washing operation; the needle head is horizontally inclined by 60 degrees.
The distance between two adjacent needles of the plate washing needle is 9mm, is exactly the same as the distance between the centers of adjacent micropores of the 96-hole plate, and is used for washing the 96-hole plate (comprising an enzyme label plate and a luminescent plate).
The bottle body of the liquid storage bottle is made of soft plastic materials, so that the liquid storage bottle is convenient to extrude; meanwhile, the bottle body is marked with scales, so that the washing liquid can be conveniently prepared by experimenters.
The liquid storage bottle can also be provided with a matched closed cover body, so that the residual washing liquid in the liquid storage bottle can be conveniently sealed and stored, and the waste is avoided.
Compared with the prior art, the utility model discloses following beneficial effect has:
the plate washing needles of the manual plate washing device are provided with a plurality of plate washing needle heads in a single row, and the distance between the adjacent needle heads corresponds to the distance between the adjacent holes of the microporous plate, so that an operator can process samples in a row unit, the primary processing amount is increased, the working efficiency is improved, and the relatively constant washing condition of the samples in the same row can be ensured; and each plate washing needle head is provided with a single-needle switch, and an operator can open the corresponding switch according to the required needle number to carry out plate washing operation.
The liquid storage bottle body is soft plastics material, convenient extrusion, just the liquid storage bottle sets up the pneumatic valve, opens during the washing the pneumatic valve through reducing the inside and outside pressure differential of liquid storage bottle for the extrusion operation is more labour saving and time saving, the effectual efficiency that has improved.
The connecting pipe is provided with the one-way water outlet valve, so that liquid in the liquid storage bottle only can not flow out, the liquid in the plate washing needle is prevented from flowing back into the liquid storage bottle after the washing operation is finished, and the washing liquid in the bottle is effectively prevented from being polluted.
Drawings
FIG. 1 is the overall structure diagram of the present invention
FIG. 2 is a partial front view of the pin header of the present invention
FIG. 3 is a partial side view of the pin header of the present invention
FIG. 4 is a partial front view of the one-way water outlet valve of the present invention
Description of the symbols in the drawings:
1 liquid storage bottle
2 plate washing needle
3 connecting pipe
4 one-way air inlet valve
5 connecting cover
6 one-way water outlet valve
7 single-needle switch
Detailed Description
The technical solution of the present invention will be described clearly and completely below, and it should be understood that the described embodiments are some, but not all embodiments of the present invention. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.
The embodiment of the utility model provides a manual plate washing device, as shown in figure 1, comprising a liquid storage bottle 1, a plate washing needle 2, a connecting pipe 3 and a connecting cover 5; a detachable connecting cover 5 is arranged on the bottle mouth of the liquid storage bottle 1, the plate washing needle 2 is arranged on the connecting cover 5 through a connecting pipe 3, the plate washing needle 2, the connecting pipe 3 and the connecting cover 5 are arranged into a whole, and the plate washing needle and the connecting pipe can be arranged and detached on the bottle mouth along with the connecting cover 5; the connecting pipe 3 is provided with a one-way water outlet valve 6, and the lower end of the bottle body of the liquid storage bottle 1 is provided with a one-way air inlet valve 4.
As shown in fig. 2 to 3, the plate washing needle 2 is formed by arranging a plurality of plate washing needle heads in a single row, in this embodiment, the plate washing needle 2 has eight plate washing needle heads, and the needle heads are horizontally inclined at 60 degrees; each needle head is provided with an independent single-needle switch 7 so that an operator can open the corresponding switch according to the required needle number to carry out plate washing operation; in the present embodiment, the single-needle switch 7 is a "straight" valve switch, but in other embodiments, other types are also possible. The distance between two adjacent needles is 9mm, the distance is exactly the same as the distance between the centers of the adjacent micropores of the 96-hole plate, and the micro-needle array can be suitable for microporous plates with the same hole distance of the 96-hole ELISA plate; however, in other embodiments, the number of needles of the plate washing needles 2 and the distance between two adjacent needles can be designed according to the specification of the reaction plate.
The bottle body of the liquid storage bottle 1 is made of soft plastic materials, so that the liquid storage bottle is convenient to extrude; meanwhile, the bottle body is marked with scales, so that experimenters can conveniently prepare washing liquid; and the liquid storage bottle 1 is provided with the one-way air inlet valve 4, the one-way air inlet valve 4 is opened during washing, and the extrusion operation is more time-saving and labor-saving by reducing the pressure difference inside and outside the liquid storage bottle 1, so that the water outlet efficiency is effectively improved.
The liquid storage bottle 1 can also be provided with a matched closed cover body, after the experiment is finished, the residual washing liquid in the liquid storage bottle is sealed and stored for the next use, and the pollution and the waste are avoided.
The connecting pipe 3 is provided with a one-way water outlet valve 6 (as shown in fig. 4), so that the washing liquid in the liquid storage bottle 1 only can not flow in and out, and the liquid in the washing board needle 2 is prevented from flowing back into the liquid storage bottle 1 after the washing operation is finished, so that the washing liquid in the liquid storage bottle 1 is prevented from being polluted.
When in use, the washing liquid is filled into the liquid storage bottle 1, the connecting cover 5 is screwed on, the connecting pipe 3 is arranged at the opening of the connecting cover 5, and the washing board needle 2 is connected; when washing, the liquid storage bottle 1 is held slightly obliquely, the one-way water outlet valve 6 and the one-way air inlet valve 4 are opened, the plate washing needle 2 is opened and closed in sequence as required, the liquid storage bottle body is slightly pressed with force, and the washing liquid flows out from the plate washing needle, so that the reaction plate can be washed.
After washing is finished, the one-way water outlet valve 6 and the one-way air inlet valve 4 are closed and opened, the plate washing needle 2, the connecting pipe 3 and the connecting cover 5 are removed, and a matched closed cover body is installed at the bottle opening of the liquid storage bottle 1, so that the unused washing liquid can be sealed.
In summary, the above embodiments are only preferred embodiments of the present invention, and are not intended to limit the scope of the present invention, and any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention should be included in the scope of the present invention.
Claims (8)
1. A manual plate washing device is characterized by comprising a liquid storage bottle, a plate washing needle, a connecting pipe and a connecting cover; the plate washing needle is arranged above the connecting cover through the connecting pipe and forms a whole, and the whole is detachably arranged at the bottle mouth of the liquid storage bottle through the connecting cover; the connecting pipe is provided with a one-way water outlet valve; the lower end of the liquid storage bottle body is provided with a one-way air inlet valve.
2. The manual plate washing device according to claim 1, wherein the plate washing needles are arranged in a single row of a plurality of plate washing needle heads.
3. A manual plate washing device according to claim 2, characterized in that a separate switch is provided on each needle of the plate washing needles.
4. The manual plate washing device according to claim 2, wherein the plate washing needle has eight plate washing needle heads.
5. The manual plate washing device according to any one of claims 1-4, wherein the needle head of the plate washing needle is horizontally inclined by 60 °.
6. The manual plate washing device according to claim 2, wherein the distance between two adjacent needles of the plurality of plate washing needles is 9 mm.
7. The manual plate washing device is characterized in that the bottle body of the liquid storage bottle is made of soft plastic.
8. The manual plate washing device is characterized in that the bottle body of the liquid storage bottle is provided with scale marks.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921139588.3U CN210876440U (en) | 2019-07-19 | 2019-07-19 | Manual plate washing device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921139588.3U CN210876440U (en) | 2019-07-19 | 2019-07-19 | Manual plate washing device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN210876440U true CN210876440U (en) | 2020-06-30 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201921139588.3U Active CN210876440U (en) | 2019-07-19 | 2019-07-19 | Manual plate washing device |
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| CN (1) | CN210876440U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113102342A (en) * | 2021-03-18 | 2021-07-13 | 高林 | Perforated plate cleaning device for enzyme-linked immunosorbent assay |
-
2019
- 2019-07-19 CN CN201921139588.3U patent/CN210876440U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113102342A (en) * | 2021-03-18 | 2021-07-13 | 高林 | Perforated plate cleaning device for enzyme-linked immunosorbent assay |
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