CN208302806U - High leakproof micro-fluidic chip based on the flowing of active control fluid - Google Patents
High leakproof micro-fluidic chip based on the flowing of active control fluid Download PDFInfo
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- CN208302806U CN208302806U CN201820476749.7U CN201820476749U CN208302806U CN 208302806 U CN208302806 U CN 208302806U CN 201820476749 U CN201820476749 U CN 201820476749U CN 208302806 U CN208302806 U CN 208302806U
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Abstract
The utility model discloses the high leakproof micro-fluidic chips flowed based on active control fluid, including chip body；Sample introduction module, micro-fluidic runner, reaction module and waste liquid module are respectively arranged on chip body；The sample outlet end of sample introduction module is connected to by micro-fluidic runner with reaction module, waste liquid sequence of modules；The sample introduction end of the sample introduction module passes through external flow path interface and external fluid communication；The chip body includes the upper layer chip that can be overlapped into one up and down, lower layer chip, and the upper surface of upper layer chip is covered with one layer of sealing paster；The front setting leakproof blotting paper putting groove of chip on upper layer, and air hole is set in the slot bottom of the leakproof blotting paper putting groove, simultaneously in the front setting waste liquid pool of lower layer chip, and blotting paper is put in waste liquid pool and leakproof blotting paper putting groove, therefore, micro-fluidic chip described in the utility model has excellent leak preventing effect.
Description
Technical field
The utility model relates to a kind of micro-fluidic chip, especially a kind of high leakproof based on the flowing of active control fluid is micro-
Fluidic chip.
Background technique
Immune lateral chromatography diagnostic techniques is suitble to the real-time test in multiplicity as a kind of stabilization and practical technology
(POCT) or scene uses.
In immunochromatography reaction system, causes CV big due to system, be unable to reach accurate quantification.And it is based on miniflow
The immune diagnostic method of control technology can effectively avoid the above problem.
It is micro-fluidic and be divided to passive type and two kinds active.Passive type is micro-fluidic still to need capillary power to reach liquid
Forward lateral chromatography.But due to the viscosity of different samples especially whole blood sample difference, cause flow rate of liquid without legally constituted authority
One.
It is active micro-fluidic it is possible to prevente effectively from the above problem, forward thrust can be given, liquid be made uniformly to flow forward
It is dynamic, it avoids because of test value difference caused by different in flow rate.
Active micro-fluidic power have centrifugation power drive, electrowetting driving, pressure-driven (electrolysis pump, compressed gas pump,
Chemical breakdown pump, the driving of direct draught head)
But if to achieve the purpose that arbitrarily to control liquid velocity, not only to have motive force, also to there is valve control,
Also there is anti-return so as not to liquid, because pressure removes, reflux is gone back.
Existing the relevant technologies, the production of chip and the application of chip, please refer to following patent:
1) a kind of micro-fluidic chip of CN203899622U
2) the micro-fluidic bedside's quick diagnosis reagent kit of CN106353491A
3) CN205941345U is used for the micro-fluidic chip of biological detection
4) CN2017105313010
Wherein, patent 2) and 3) in, the sample liquid being added in chip is not quantified, and to realize quantitative inspection
It surveys, it is necessary to realize to the quantitative of the sample of addition and be pre-placed quantifying in the reagent in chip.
Patent 1), 2) and 3) in be all not directed to the monitoring of accurate location when liquid flows in the chips, it is in other words, above-mentioned
The chip of 3 patents before last testing result to fluid, do not supervise in the chip by the filling behavior of channel or cavity
It surveys.
Patent 4) in chip be divided into three chips of upper, middle and lower, and chip has sample outlet hole, cleaning fluid apertures and colour developing fluid apertures
Deng complicated structure.The porous unifications such as sample outlet hole, cleaning fluid apertures and colour developing fluid apertures are kept chip structure simple, are conducive to by this chip
Industrialization processing.Only upper layer and lower layer chip simultaneously, is further simplified framework.Reduce processing cost.
In addition, in the detection of existing micro-fluidic chip, to prevent using the developing solution rotten because of light pollution is arrived, generally
It is to be realized by regularly replacing.But it due to the difference of developing solution Conservation environment, uses and qualitative change occurs because of light pollution
Developing solution carry out chip detection, and the phenomenon that happen occasionally.Therefore, how to avoid using to this rotten developing solution,
Colour developing result distortion after causing sample to be tested to react, influences the accuracy of chip test result, is to improve chip test result
One major issue of accuracy rate.
In addition, if will just develop the color fluid exchange, will also result in the waste of developing solution simply because developing solution background values is high.
Utility model content
In view of the shortcomings of the prior art, the utility model provides a kind of high leakproof miniflows based on the flowing of active control fluid
Control chip.Leakproof blotting paper putting groove is arranged in the front of upper layer chip in it, and sets in the slot bottom of the leakproof blotting paper putting groove
Air hole is set, while in the front setting waste liquid pool of lower layer chip, and is put in waste liquid pool and leakproof blotting paper putting groove
Blotting paper, therefore, micro-fluidic chip described in the utility model have excellent leak preventing effect.
To realize above-mentioned technical purpose, the utility model takes the following technical solution:
A kind of high leakproof micro-fluidic chip based on the flowing of active control fluid, including chip body；Divide on chip body
It is not provided with sample introduction module, micro-fluidic runner, reaction module and waste liquid module；The sample outlet end of sample introduction module passes through miniflow flow control
Road is connected to reaction module, waste liquid sequence of modules；The sample introduction end of the sample introduction module passes through external flow path interface and external flow path
Connection；It is characterized by: the chip body includes the upper layer chip that can be overlapped into one up and down, lower layer chip, and upper layer
The upper surface of chip is covered with one layer of sealing paster；Sample outlet end, the reaction of sample introduction module are respectively arranged in the lower layer chip
The waste liquid pool of the reaction tank of module, waste liquid module；Upper layer chip be arranged at position corresponding with the sample outlet end of sample introduction module into
Reaction tank cover board is arranged at position corresponding with reaction tank, sets at position corresponding with waste liquid pool for the sample introduction end of egf block
Waste liquid pool cover board is set, while leakproof blotting paper putting groove is set in the outside of reaction tank cover board；
Waste liquid pool can form waste liquid chamber with waste liquid pool cover plate splice；
Reaction tank can form quantitative reaction chamber with reaction tank cover plate splice；
Leakproof blotting paper putting groove can be spliced into one along chip sheet on the horizontal plane of chip body with waste liquid pool
The groove body of the entire circle of outer rim one setting of body, and the slot bottom of leakproof blotting paper putting groove is provided with air hole；The leakproof blotting paper
Waterproof paper is equipped in putting groove and waste liquid pool, and there are gaps between the waterproof paper in waste liquid pool and waste liquid pool pool wall.
The waste liquid pool is evenly distributed with several waste liquid pool positioning columns at the position of pool wall, along pool wall, and
Waste liquid pool cover board then configures a waste liquid pool location hole relative to each waste liquid pool positioning column；Waste liquid pool and waste liquid pool cover board are logical
It is integral to cross waste liquid pool positioning column, waste liquid pool location hole snapping；The blotting paper being placed in waste liquid pool is in the positioning of each waste liquid pool
In the inner ring that column surrounds.
Be respectively arranged on the upper layer chip the first anti-return runner, the second anti-return runner, third anti-return runner,
4th anti-return channel；The sample outlet hole of sample introduction module is connect by the first anti-return runner with background detection window；Background detection
Window is connected to by the second anti-return runner with reaction tank；Reaction tank is connected to by third anti-return runner with valving；And
Valving then passes through the 4th anti-return channel and is connected to waste liquid pool.
The external flow path includes external gas circuit, external cleaning fluid path, external colour developing fluid path；External gas circuit, external cleaning
Fluid path, external colour developing fluid path, which merge, uses the same external flow path interface；The sample outlet end of the sample introduction module is provided with a cross
The sample outlet hole of the rounded setting in section, and the periphery of sample outlet hole is provided with a tapering transition joint face.
The reaction tank has fluid inlet, fluid outlet；The fluid inlet of reaction tank, fluid outlet respectively with react
Place plane of the pond on chip body is perpendicular.
Fluid inlet, the fluid outlet of reaction tank are connected to reaction tank by a gradient chamfered transition respectively.
The circular in cross-section setting of quantitative-reaction chamber.
According to above-mentioned technical solution, relative to present technology, the utility model have it is following the utility model has the advantages that
1, leakproof blotting paper putting groove is arranged in the front of upper layer chip in the utility model, and puts in the leakproof blotting paper
Air hole is arranged in the slot bottom of slot, while in the front setting waste liquid pool of lower layer chip, and puts in waste liquid pool and leakproof blotting paper
Blotting paper is put in slot, therefore, micro-fluidic chip described in the utility model has excellent leak preventing effect.
2, a sample holes are only arranged in the utility model on chip body, so that sample to be tested, cleaning solution, labelled antibody
And developing solution can be entered in the runner of chip body by the sample holes, respectively correspondingly complete the sample-adding of chip body
Operation, cleaning operation, marking antibody operation and color operation, i.e., by sample outlet hole in the prior art, plus cleaning fluid apertures, plus
Labelled antibody hole and plus colour developing fluid apertures close four be one, therefore, the utility model have structure be simply conducive to industrialization processing it is excellent
Point.In addition, passing through the control of instrument software and hardware, the two-step method operation of double antibodies sandwich can be reached.It avoids because caused by one-step method
Hook effect.
3, the utility model is integrated with background detection window in chip body, can pass through during color developing detection
The developing solution numerical value in the quantitative chamber of developing solution numerical value and reaction-after comparing the detection of background detection window, obtains final detection
As a result.Therefore, the utility model avoids that chemiluminescence developing solution is unstable, and in light pollution, numerical value can be got higher, and influences
The generation of the normal reading value phenomenon of product.
4, in quantitative-reaction chamber described in the utility model, fluid inlet, fluid outlet set-up mode (with it is quantitative-anti-
Answer place plane of the chamber on chip body perpendicular), it on the one hand avoids liquid because of capillary power, flows out quantitative-reaction chamber,
Have the function of anti-return, it on the other hand, can be to avoid if micro-fluidic chip described in the utility model is the chip based on magnetic bead
The magnetic particle included in quantitative-reaction chamber loses problem.Slope between liquid inlet and outlet simultaneously and quantitative-reaction chamber,
It is recycled under quantitative-reaction chamber strong magnetism at bottom absorption conducive to the magnetic particle lost on a small quantity, further avoids magnetic particle loss
Generation；The generation of bubble when gentle slope can also be moved to avoid liquid flow.
5, the utility model only combines the mode for sticking on film with upper layer and lower layer chip, by complicated chip structure, simplification.
Cost is significantly reduced, the cost including chip material cost, the cost of mold and processing.
Detailed description of the invention
Fig. 1 is the decomposition texture schematic diagram of the utility model micro-fluidic chip；
1- seals paster；The upper layer 2- chip；3- lower layer chip；The external flow path interface of 4-；The external valve compression bar of 5-；6- is conductive
Probe；7- leakproof blotting paper；8- conductive rubber；9- blotting paper；
Fig. 2 is the structural schematic diagram of upper layer chip front side in micro-fluidic chip described in the utility model；
20- valving；The upper layer 21- chip sample introduction position；22- the first anti-return runner；23- the second anti-return runner；
The upper layer 24- chip reaction chamber position；25- third anti-return runner；26- leakproof blotting paper puts frame；27- air hole；28- the 4th
Anti-return channel；
Fig. 3 is the structural schematic diagram of upper layer chip back described in the utility model；
27- air hole；29- waste liquid pool cover board；29-1, waste liquid pool location hole；The liquid out of 22-1, the first anti-return runner
Hole；The inlet opening of 23-1, the second anti-return runner；24-1, reaction tank cover board；The inlet of 25-1, third anti-return runner；
The inlet of 20-1, valving；The liquid outlet of 20-2, valving；The inlet of 28-1, the 4th anti-return runner.
Fig. 4 is the positive structural schematic diagram of lower layer chip described in the utility model；
Fig. 5 be the utility model it is quantitative-structural schematic diagram of reaction chamber；
In Fig. 4, Fig. 5: 31, the sample outlet hole of sample introduction module；32, background detection window；33, quantitative-reaction chamber；331, fluid
Entrance；332, fluid outlet；333, slope；34, waste liquid pool；341, positioning column.
Specific embodiment
The following will be combined with the drawings in the embodiments of the present invention, carries out the technical scheme in the embodiment of the utility model
Clearly and completely describe, it is clear that the described embodiments are only a part of the embodiments of the utility model, rather than whole
Embodiment.Be to the description only actually of at least one exemplary embodiment below it is illustrative, never as to this is practical
Novel and its application or any restrictions used.Based on the embodiments of the present invention, those of ordinary skill in the art are not having
Every other embodiment obtained under the premise of creative work is made, is fallen within the protection scope of the utility model.Unless
In addition it illustrates, component and the positioned opposite of step, expression formula and the unlimited system of numerical value otherwise illustrated in these embodiments
The scope of the utility model.Simultaneously, it should be appreciated that for ease of description, the size of various pieces shown in attached drawing is not
It is to be drawn according to actual proportionate relationship.It is possible for technology known to person of ordinary skill in the relevant, method and apparatus
It is not discussed in detail, but in the appropriate case, the technology, method and apparatus should be considered as authorizing part of specification.
In shown here and discussion all examples, any occurrence should be construed as merely illustratively, not as limit
System.Therefore, the other examples of exemplary embodiment can have different values.
For ease of description, spatially relative term can be used herein, as " ... on ", " ... top ",
" ... upper surface ", " above " etc., for describing such as a device shown in the figure or feature and other devices or spy
The spatial relation of sign.It should be understood that spatially relative term is intended to comprising the orientation in addition to device described in figure
Except different direction in use or operation.For example, being described as if the device in attached drawing is squeezed " in other devices
It will be positioned as " under other devices or construction after part or construction top " or the device of " on other devices or construction "
Side " or " under other devices or construction ".Thus, exemplary term " ... top " may include " ... top " and
" in ... lower section " two kinds of orientation.The device can also be positioned with other different modes and (is rotated by 90 ° or in other orientation).
As shown in Fig. 1 to 5, the high leakproof micro-fluidic chip described in the utility model based on the flowing of active control fluid,
Including chip body；Sample introduction module, micro-fluidic runner, reaction module and waste liquid module are respectively arranged on chip body；Into
The sample outlet end of egf block is connected to by micro-fluidic runner with reaction module, waste liquid sequence of modules；The sample outlet end of the sample introduction module
Developing solution background detection window is provided on micro-fluidic runner between reaction module.
The sample introduction end of the sample introduction module passes through external flow path interface and external fluid communication；The external flow path includes outer
Connect gas circuit, external cleaning fluid path, external colour developing fluid path；External gas circuit, external cleaning fluid path, external colour developing fluid path merge using same
One external flow path interface；The sample outlet end of the sample introduction module is provided with the sample outlet hole of circular in cross-section setting, and goes out
The periphery in sample hole is provided with a tapering transition joint face.It can thus be appreciated that: the utility model is by well plus cleans fluid apertures, mark-on
Remembering antibody hole and colour developing fluid apertures is added to close four is one, and structure is simply conducive to industrialization and processes.Meanwhile passing through the control of instrument software and hardware
System can reach the two-step method operation of double antibodies sandwich.It avoids because of hook effect caused by one-step method.
The reaction module includes quantitative-reaction chamber, which has fluid inlet, fluid outlet；Quantitative-
Fluid inlet, the fluid outlet of reaction chamber are perpendicular with quantitative-place plane of the reaction chamber on chip body respectively.
Fluid inlet, the fluid outlet of quantitative-reaction chamber are connected to quantitative-reaction by a gradient chamfered transition respectively
Chamber.
The circular in cross-section setting of quantitative-reaction chamber.
The chip body includes the upper layer chip that can be overlapped into one up and down, lower layer chip, and upper layer chip is upper
Surface is covered with one layer of sealing paster；Be respectively arranged in the lower layer chip sample outlet end of sample introduction module, reaction module it is anti-
The waste liquid pool of Ying Chi, waste liquid module；Sample introduction module is arranged in upper layer chip at position corresponding with the sample outlet end of sample introduction module
Reaction tank cover board is arranged in sample introduction end at position corresponding with reaction tank, and waste liquid pool is arranged at position corresponding with waste liquid pool
Cover board, while leakproof blotting paper putting groove is set in the outside of reaction tank cover board；Leakproof blotting paper putting groove, in chip body
A groove body along the entire circle of outer rim one setting of chip body, and leakproof blotting paper can be spliced on horizontal plane with waste liquid pool
The slot bottom of putting groove is provided with air hole.Air hole is arranged in upper layer chip the utility model, and is provided in the position anti-
Blotting paper putting groove is leaked, leak preventing effect is further improved.
The waste liquid pool is evenly distributed with several waste liquid pool positioning columns at the position of pool wall, along pool wall, and
Waste liquid pool cover board then configures a waste liquid pool location hole relative to each waste liquid pool positioning column；Waste liquid pool and waste liquid pool cover board are logical
It is integral to cross waste liquid pool positioning column, waste liquid pool location hole snapping；The blotting paper being placed in waste liquid pool is in the positioning of each waste liquid pool
In the inner ring that column surrounds.Blotting paper absorbs liquid, prevents chip body leakage；Blotting paper does not completely fill out waste liquid pool simultaneously,
Reserved gas passage, avoids liquid in blotting paper from being blown out.
Be respectively arranged on the upper layer chip the first anti-return runner, the second anti-return runner, third anti-return runner,
4th anti-return channel；The sample outlet hole of sample introduction module is connect by the first anti-return runner with background detection window；Background detection
Window is connected to by the second anti-return runner with reaction tank；Reaction tank is connected to by third anti-return runner with valving；And
Valving then passes through the 4th anti-return channel and is connected to waste liquid pool.
In summary, it is known that micro-fluidic chip described in the utility model has the advantage that
The utility model creativeness increases developing solution background detection window, can detect developing solution specific value in time, and
Reactive tank detect numerical value after, reduce the background values of developing solution, timely and effectively reject developing solution it is unstable caused by background
Interference value.
The quantitative chamber of reaction-is made into circle by the utility model, is conducive to liquid blending.And it is creative by liquid inlet and
Outlet is made into the design vertical with reacting-quantifying chamber, and the magnetic particle included is avoided to lose problem.Simultaneously liquid inlet and outlet and
There is slope between the quantitative chamber of reaction-, recycles benefit under the quantitative strong magnetic suck in bottom of chamber portion of reaction-conducive to the magnetic particle lost on a small quantity
With, further avoid magnetic particle loss generation.
The utility model only combines the mode for sticking on film with upper layer and lower layer chip, by complicated chip structure, simplification.It is aobvious
Work reduces cost, the cost including chip material cost, the cost of mold and processing.
Coated antibody and labelled antibody are creatively cleverly placed in same reactive tank by the utility model, simplify examination
Agent operating process, while having space partition again from each other, effectively avoid non-specific binding.Or labelled antibody is placed on core
In the outer reagent bottle of piece, reactive tank is divided into two parts up and down, is individually positioned on different layers chip, improves reagent coating in reactive tank
Area, that is, upper layer and lower layer chip can coated antibody, antibody load capacity doubles, and effectively improves product sensitivity.
The utility model creatively devises valving, back-flow preventer, and combines gas circuit forward as liquid
Motive force can arbitrarily control liquid velocity in runner, and can be fixed in a certain region, carry out incubation reaction.Reach real
Micro-fluidic purpose.
The utility model can reach the quantitative sample-adding in this meaning by quantitative slot,
Water suction material is added in the utility model in waste liquid pool, plays the role of preventing waste liquid spluttering, while cleverly setting
Gas passage is counted, the devices such as middle layer chip blotting paper effectively prevent liquid leakage.Keep simple production process practical.
The utility model is having sealing ring made of conductive rubber and device capacitances detection probe to play together, liquid
The detection of flow regime.
For the utility model by quantitative slot, marker slot and reactive tank conjunction three are one, not only effectively can be quantitatively loaded, but also
The different zones that labelled antibody and coated antibody can be placed in same slot effectively avoid non-specific binding, improve signal-to-noise ratio.
It designs simultaneously simple and practical.
The utility model connects chip by fluid path, cleans reactive tank, and reaction background is effectively reduced, and it is sensitive to improve product
Degree.
Another technical purpose of the utility model is to provide a kind of detection method based on above-mentioned micro-fluidic chip, including with
Lower step:
1) in reagent chip ontology inserting instrument, information above is read；
2) sample is added in the sample outlet hole of sample introduction module using pipettor；
3) chip body enters instrument internal, is incubated for 2 minutes；
4) instrument and chip contact device are combined；Chip contact device includes external flow circuit device, with conductive rubber
The valve actuation head of probe；
5) gas path device in external flow circuit device is slowly inflated, and sample is pushed to be moved along；
6) sample enters background detection window by the first anti-return runner under the promotion of air-flow；
7) sample continues forward, quantitative intracavitary into reaction-by the second anti-return runner；
8) sample continues forward, and by third anti-return runner, into valving, sample is led in valve once contacting
Electric rubber, capacitance variations can touch valve switch, close valve；Air circuit breaker is simultaneously closed off, pressurization is stopped；Reaction-is fixed at this time
It measures intracavitary fluid and is in static condition；
9) instrument and chip contact device separation, mix 3-10 minutes, are immunoreacted；
10) instrument and chip contact device combine, and the gas path device work in external flow circuit device passes through gas push sample
This is moved forward in micro-fluidic runner, is dried up the residual sample in the sample outlet hole and micro-fluidic runner of sample introduction module, is led at this time
Electric rubber probe data becomes original value again, closes gas path device；
11) the cleaning fluid path device starting in external flow circuit device, cleaning solution are entered micro- by the sample outlet hole of sample introduction module
In flow control runner；
12) cleaning solution continue to move forward into reaction-quantify module reaction-it is quantitative intracavitary；
13) cleaning solution continues forward, and into valving, cleaning solution once contacts the conductive rubber in valving,
Capacitance variations can touch valve switch, close valving, can keep away in the subsequent 4th anti-return runner of valving at this time
Exempt from the case that valving closes not in time, the problem of blowing away liquid；Gas path device is simultaneously closed off, pressurization is stopped；
14) it mixes 1-3 minutes, is cleaned；
15) gas path device works, and gas push cleaning solution moves forward, and dries up the liquid in sample outlet hole and micro-fluidic runner
Body, conductive rubber probe data becomes original value again at this time, closes gas path device；
16) repetitive operation 11) to 15) step 3-8 times；
17) start the developing solution fluid path device in external flow circuit device, developing solution is entered by the sample outlet hole of sample introduction module
In micro-fluidic runner；
18) developing solution continues after being advanced past background detection window, and the reaction-into the quantitative module of reaction-is quantitative intracavitary；
19) developing solution continues forward, and into valving, developing solution once contacts the conductive rubber in valving,
Capacitance variations can touch valving, close valving；Gas path device is simultaneously closed off, pressurization is stopped；
20) it mixes 3-8 minutes, carries out chromogenic reaction；
21) instrument and chip contact device separation；
22) instrument reads data, by analyzing, calculating the colour developing data of background detection window, the quantitative chamber of reaction-, obtains
Testing result；
23) instrument exits chip, completes detection.
Claims (7)
1. a kind of high leakproof micro-fluidic chip based on the flowing of active control fluid, including chip body；On chip body respectively
It is provided with sample introduction module, micro-fluidic runner, reaction module and waste liquid module；The sample outlet end of sample introduction module passes through micro-fluidic runner
It is connected to reaction module, waste liquid sequence of modules；The sample introduction end of the sample introduction module is connected by external flow path interface and external flow path
It is logical；It is characterized by: the chip body includes the upper layer chip that can be overlapped into one up and down, lower layer chip, and upper layer core
The upper surface of piece is covered with one layer of sealing paster；Sample outlet end, the reaction mould of sample introduction module are respectively arranged in the lower layer chip
The waste liquid pool of the reaction tank of block, waste liquid module；Sample introduction is arranged in upper layer chip at position corresponding with the sample outlet end of sample introduction module
Reaction tank cover board is arranged in the sample introduction end of module at position corresponding with reaction tank, is arranged at position corresponding with waste liquid pool
Waste liquid pool cover board, while leakproof blotting paper putting groove is set in the outside of reaction tank cover board；
Waste liquid pool can form waste liquid chamber with waste liquid pool cover plate splice；
Reaction tank can form quantitative reaction chamber with reaction tank cover plate splice；
Leakproof blotting paper putting groove, can be spliced into waste liquid pool on the horizontal plane of chip body one it is whole along chip body
The groove body of a circle of outer rim one setting, and the slot bottom of leakproof blotting paper putting groove is provided with air hole；The leakproof blotting paper is put
Waterproof paper is equipped in slot and waste liquid pool, and there are gaps between the waterproof paper in waste liquid pool and waste liquid pool pool wall.
2. the high leakproof micro-fluidic chip according to claim 1 based on the flowing of active control fluid, it is characterised in that: described
Waste liquid pool is evenly distributed with several waste liquid pool positioning columns at the position of pool wall, along pool wall, and waste liquid pool cover board is then
A waste liquid pool location hole is configured relative to each waste liquid pool positioning column；Waste liquid pool and waste liquid pool cover board are positioned by waste liquid pool
Column, waste liquid pool location hole snapping are integral；The blotting paper being placed in waste liquid pool is in the inner ring that each waste liquid pool positioning column surrounds
In.
3. the high leakproof micro-fluidic chip according to claim 2 based on the flowing of active control fluid, it is characterised in that: described
It is logical that the first anti-return runner, the second anti-return runner, third anti-return runner, the 4th anti-return are respectively arranged on the chip of upper layer
Road；The sample outlet hole of sample introduction module is connect by the first anti-return runner with background detection window；Background detection window passes through second
Anti-return runner is connected to reaction tank；Reaction tank is connected to by third anti-return runner with valving；And valving then leads to
The 4th anti-return channel is crossed to be connected to waste liquid pool.
4. the high leakproof micro-fluidic chip according to claim 2 based on the flowing of active control fluid, it is characterised in that: described
External flow path includes external gas circuit, external cleaning fluid path, external colour developing fluid path；External gas circuit, external cleaning fluid path, external colour developing
Fluid path, which merges, uses the same external flow path interface；The sample outlet end of the sample introduction module is provided with a circular in cross-section setting
Sample outlet hole, and the periphery of sample outlet hole is provided with a tapering transition joint face.
5. the high leakproof micro-fluidic chip according to claim 4 based on the flowing of active control fluid, it is characterised in that: described
Reaction tank has fluid inlet, fluid outlet；The fluid inlet of reaction tank, fluid outlet are respectively with reaction tank in chip body
On place plane it is perpendicular.
6. the high leakproof micro-fluidic chip according to claim 5 based on the flowing of active control fluid, it is characterised in that: reaction
Fluid inlet, the fluid outlet in pond are connected to reaction tank by a gradient chamfered transition respectively.
7. the high leakproof micro-fluidic chip according to claim 6 based on the flowing of active control fluid, it is characterised in that: fixed
The setting of amount-reaction chamber circular in cross-section.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109569754A (en) * | 2019-01-09 | 2019-04-05 | 南京岚煜生物科技有限公司 | Single index micro-fluidic chip and its production method, application method |
| CN113522387A (en) * | 2021-07-23 | 2021-10-22 | 江苏溢康辰医疗科技有限公司 | Bidirectional active micro-fluidic chip and application method thereof |
-
2018
- 2018-04-04 CN CN201820476749.7U patent/CN208302806U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109569754A (en) * | 2019-01-09 | 2019-04-05 | 南京岚煜生物科技有限公司 | Single index micro-fluidic chip and its production method, application method |
| CN109569754B (en) * | 2019-01-09 | 2021-09-07 | 南京岚煜生物科技有限公司 | Single-index microfluidic chip and production method and use method thereof |
| CN113522387A (en) * | 2021-07-23 | 2021-10-22 | 江苏溢康辰医疗科技有限公司 | Bidirectional active micro-fluidic chip and application method thereof |
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