CN205483748U - Immunostaining machine - Google Patents
Immunostaining machine Download PDFInfo
- Publication number
- CN205483748U CN205483748U CN201620126863.8U CN201620126863U CN205483748U CN 205483748 U CN205483748 U CN 205483748U CN 201620126863 U CN201620126863 U CN 201620126863U CN 205483748 U CN205483748 U CN 205483748U
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- China
- Prior art keywords
- hydraulic cylinder
- slide glass
- scanning system
- pathological section
- immunostaining
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Abstract
The utility model relates to an immunostaining machine, it includes: frame and strutting arrangement, immunostaining device, image acquisition device, heating device and control system. Compared with the prior art, the utility model beneficial effect lie in carrying out a lot of immunostaining to same tissue slice, can carry out immunostaining and image acquisition in turn to same tissue slice, the situation is expressed to the multiple antigen that helps studying same cell.
Description
Technical field
This utility model belongs to detection equipment technical field, particularly relates to a kind of immunostaining machine being applied in Hospital Pathological Department tissue slice.
Background technology
Immunostaining is the principle utilizing antigen to be combined with antibody specificity, the expression of detection specific protein (antigen), it is to find body one of changes of function most common method under physiology or pathological state, is most commonly used that immunohistochemical staining and immunofluorescence dyeing.In experimentation, it is often necessary to research at least two antigen/protein expression in same cell or tissue specimen, in order to the location of clear and definite each of which or coexpression situation.The method being most frequently with at present is flow cytometry, but the cell concentration that this technology needs is bigger, and it is used for Flow cytometry poor effect through fixing and paraffin-embedded tissue, it is impossible to accurately show that various antigens are at tissue and cell natural distribution and status in vivo.If cell concentration to be detected is few in tissue specimen amount few (such as cytological samples obtained by fine-needle aspiration) or tissue, then it is difficult to carry out Flow cytometry.Study the expression of multiple antigen by tissue sections same specimen being carried out repeatedly immunostaining, then can solve this problem.Such as, after tissue slice is carried out the immunofluorescence dyeing of antigen A, due to being combined into and unstable Non-covalent binding of antigen and antibody, tissue can be put in buffer, suitably heating, the antibody and the corresponding developer that have combined with antigen A just can be made to separate, the immunofluorescence dyeing of antigen B can be carried out again, use same method can also carry out antigens c, the dyeing of antigen D.At present the Multiple immunizations staining technique for tissue slice occurs, i.e. same tissue slice is once dyeed, study multiple antigen presentation, such as a kind of Double-immunofluorescent labeling method (CN201410099873.2) based on same Species origin first antibody, the shortcoming of this method is, in specific implementation process, needing the antigen being marked is not only often two kinds, it it is in most cases 5-10 kind, even reach more than 20 kinds, this multiple staining method can not meet needs, needs repeatedly to dye.Certainly, multiple staining can be combined with repeatedly colouring method, work efficiency can be improved.It addition, in repeatedly dyeing course, take pictures after needing tissue slice is dyeed every time, stained photographs repeatedly is compared, just can understand which antigen same cell expresses actually, thus, the device provided also to be easy to take pictures under the microscope.Applicant discloses a kind of slide glass folder (201520587855.9) for tissue slice carries out repeatedly immunostaining, a kind of slide glass folder for tissue slice carries out repeatedly immunostaining being easy to craft or machine full-automatic operation is provided, the amount of labour in order to overcome manual operations to bring increases and is difficult to the deficiencies such as standardization, it is necessary to provide a kind of full-automatic immunostainer possessing repeatedly immunostaining and image collecting function.
Summary of the invention
The purpose of this utility model is to provide a kind of immunostaining machine, its technical problem to be solved is: use existing immunostainer same tissue slice cannot be carried out repeatedly immunostaining, it is impossible to same tissue slice is carried out immunostaining alternately and image acquisition.
For solving above-mentioned technical problem, this utility model provides a kind of immunostaining machine, comprising: framework and support means, immunostaining device, image collecting device, heater and control system.
Described framework and support means, by four big frames, bolt is installed and corresponding big frame is installed nut and is installed together by upper cover plate, lower cover, left cover, right cover plate, front shroud, back shroud and slide glass folder mounting sheet, formed and be divided into upper and lower two-part rectangular box-like structure with slide glass folder mounting sheet.Described slide glass folder mounting sheet is in " H " shape, including a crossbeam and two arms.
Described immunostaining device, moves carrier, upper hydraulic cylinder, longitudinal hydraulic cylinder, pipettor hydraulic cylinder, middle hydraulic cylinder, lower hydraulic cylinder, pipette slide glass, slide glass folder and vacuum pump, framework upper upper rail and framework upper lower guideway and cylindrical carrier including micropipettor, pipettor.Cylindrical carrier is arranged on position on the right side above slide glass folder mounting sheet crossbeam, is used for storing carrier fluid.Upper cover plate is opened with direction from left to right the window of a bar shaped, is used for that pipettor is installed and moves carrier at framework upper upper rail and framework upper lower guideway.Described upper hydraulic cylinder is arranged on upper cover plate from left to right, is used for promoting pipettor to move carrier left and right displacement；Longitudinal hydraulic cylinder is arranged on upper cover plate from the top down, is used for promoting pipettor mobile vehicle upper and lower displacement；Pipettor hydraulic cylinder is arranged on mobile vehicle, and micropipettor can be promoted to take liquid push rod, it is achieved micropipettor takes liquid and blows liquid function；Middle hydraulic cylinder is arranged near the position in left side below the crossbeam of slide glass folder mounting sheet from left to right, for promoting pipette slide glass to the underface of slide glass folder mounting sheet laid slide glass folder and to reset accordingly.Described slide glass folder is arranged on the left side of cylindrical carrier on slide glass folder mounting sheet.It is in the same size that 1-20 opens slide glass folder, coincide with the reserved installation position of slide glass folder mounting sheet, all slide glasses folder with equidirectional in wire neat horizontal arranged in parallel.Each hydraulic cylinder described is respectively mounted independent displacement transducer.
Described slide glass folder is as described in patent (a kind of slide glass folder for tissue slice carries out repeatedly immunostaining: 201520587855.9), it is made up of colourless transparent plastic, upper slice be sieve-like, it is that there is at least one liquid outlet at the edge of bottom sheet with the difference of a kind of slide glass folder for tissue slice being carried out repeatedly immunostaining, this liquid outlet communicates with the gap between bottom sheet with upper slice, make slice the liquid between bottom sheet can be sopped up by this hole, upper slice and bottom sheet between carry tissue slice.
Described image collecting device, including lower hydraulic cylinder, lower frame lower guideway, lower frame upper rail, digital pathological section scanning system mobile vehicle and digital pathological section scanning system.Described lower frame lower guideway, lower frame upper rail are arranged on lower cover with direction from left to right, described digital pathological section scanning system mobile vehicle is arranged on lower frame lower guideway and lower frame upper rail, described digital pathological section scanning system is arranged on digital pathological section scanning system mobile vehicle, described lower hydraulic cylinder is arranged on the left side of digital pathological section scanning system mobile vehicle, is used for promoting digital pathological section scanning system arrive slide glass folder position and reset accordingly.Described digital pathological section scanning system is with the difference of conventional digital pathological section scanning system: the microscope used is inverted microscope, it is possible to provide at least white, red, green and blue four kinds of light sources.
Described master controller obtains the displacement transducer data of each hydraulic cylinder, and is realized the accurate control of each hydraulic cylinder by the corresponding hydraulic cylinder electromagnetic valve of regulation；
Described master controller digital pathological section scanning system is moved to slide glass folder position after send instructions to scanner start scanning, the data transmission of scanning processes to PC, after one scan sample completes, scanner is moved to next sample position by master controller, and again send instruction scanner so that it is restart scanning.
Described master controller obtains temperature sensor data and realizes the control to sample environment temperature by regulation heater.Described heater is arranged in lower plate, and temperature sensor is arranged in framework, makes the temperature in framework control 37 DEG C (operating ambient temperature have to be lower than 37 DEG C).
Operation principle and process:
Operationally, the slide glass carrying tissue slice (paraffin section need to be through dehydration) is folded up the predeterminated position being placed in slide glass folder mounting sheet, carrier fluid is filled in cylindrical carrier；According to the carrier fluid position intending absorption, upper Driven by Hydraulic Cylinder pipettor moves carrier and makes micropipettor displacement in left-right direction, after arriving above cylindrical carrier, longitudinal Hydraulic Cylinder pipettor mobile vehicle makes micropipettor to bottom offset, the suction nozzle making pipettor declines arrival carrier fluid, pipettor hydraulic cylinder is pulled upwardly micropipettor and takes liquid push rod, it is achieved micropipettor takes liquid function；Then micropipettor is made to shift up by longitudinal Driven by Hydraulic Cylinder pipettor mobile vehicle, the suction nozzle making pipettor rises and leaves carrier fluid, moved carrier by upper Hydraulic Cylinder pipettor and make micropipettor displacement to the right, when mobile to the slide glass folder position intending filling carrier fluid, longitudinal Hydraulic Cylinder pipettor moves carrier makes micropipettor be moved to downwards close to slide glass folder, pushed down on micropipettor by pipettor hydraulic cylinder and take liquid push rod, liquid is filled on tissue slice accurately.After the certain time set, the underface that middle Hydraulic Cylinder pipette slide glass presss from both sides to slide glass, vacuum pump is opened, and sucks the carrier fluid in slide glass folder, and the step then can being similar to carries out the filling of lower a kind of carrier fluid.If intending carrying out image acquisition, lower Driven by Hydraulic Cylinder digital pathological section scanning system to slide glass presss from both sides position, and lower Driven by Hydraulic Cylinder digital pathological section scanning system resets after scanning completes.After completing image acquisition, then carry out next step operation.During whole, master controller accurately controls corresponding hydraulic cylinder electromagnetic valve by obtaining the displacement transducer data of each hydraulic cylinder；Accurately controlling digital pathological section scanning system to move to slide glass folder position and corresponding scanning process, the data transmission of scanning processes to PC；Obtain temperature sensor data and realize the control to sample environment temperature by regulation heater.
This utility model compared with prior art provides the benefit that: same tissue slice can be carried out repeatedly immunostaining；Same tissue slice can be carried out immunostaining alternately and image acquisition.
Accompanying drawing illustrates:
Fig. 1 is positive sectional structure chart of the present utility model；
Fig. 2 is left side view of the present utility model；
Fig. 3 is right side view of the present utility model；
Fig. 4 is that slide glass of the present utility model presss from both sides mounting sheet planform figure；
Fig. 5 is slide glass clamping structure figure of the present utility model；
Fig. 6 is each hydraulic cylinder mounting means figure of the present utility model；
Fig. 7 is control structure module schematic diagram of the present utility model.
In figure: 1, big frame installs bolt；2, big frame installs nut；3, upper hydraulic cylinder；4 ~ 7, upper hydraulic cylinder installs screw；8, framework upper upper rail；9, framework upper upper rail installs screw；10 ~ 13, longitudinal hydraulic cylinder installs screw；14~
17, longitudinal hydraulic cylinder installs screw；18, longitudinal hydraulic cylinder；19, carrier is moved；20, pipettor mobile vehicle；21, framework upper lower guideway；22, big frame installs nut；23, big frame installs bolt；24, pipettor hydraulic cylinder；25, pipettor hydraulic cylinder installs screw；26, micropipettor；27, pipettor mounting bar；28, pipettor installs screw；29, carrier fluid and cylindrical carrier；30, right cover plate；31, lower cover；32, heater；33, lower frame lower guideway；34, lower frame upper rail；35, lower frame guide rails assembling screw；36, digital pathological section scanning system mobile vehicle；37 ~ 38, digital pathological section scanning system installs screw；39, digital pathological section scanning system；40, vacuum pump mounting bracket；41, vacuum pump；42, middle hydraulic cylinder leg；43, lower hydraulic cylinder；44, middle hydraulic cylinder lateral frame；45, middle hydraulic cylinder；46, slide glass folder mounting sheet；47, imbibition house steward；48, middle hydraulic cylinder installs screw；49, pipette slide glass；50, imbibition rubber tube；51, left cover；52, upper cover plate；53, slide glass folder；54, upper slice；55, bottom sheet；56, liquid outlet；57, tissue slice.
Detailed description of the invention
Below in conjunction with the accompanying drawings embodiment of the present utility model is described further:
Framework described in the utility model and support means, by four big frames, bolt 1 is installed and corresponding big frame is installed nut 2 and is installed together by upper cover plate 52, lower cover 31, left cover 51, right cover plate 30, front shroud, back shroud and slide glass folder mounting sheet 46, formed and be divided into upper and lower two-part rectangular box-like structure with slide glass folder mounting sheet 46.Described slide glass folder mounting sheet 46 is in " H " shape, including a crossbeam and two arms.
Described immunostaining device, moves carrier 19, upper hydraulic cylinder 3, longitudinal hydraulic cylinder 18, pipettor hydraulic cylinder 24, middle hydraulic cylinder 45, lower hydraulic cylinder 43, pipette slide glass 49, slide glass folder 53 and vacuum pump 41, framework upper upper rail 8 and framework upper lower guideway 21 and cylindrical carrier 29 including micropipettor 26, pipettor.Cylindrical carrier 29 is arranged on position on the right side above slide glass folder mounting sheet 46 crossbeam.Upper cover plate 52 is opened with direction from left to right the window of a bar shaped, is used for that pipettor is installed and moves carrier 19 at framework upper upper rail 8 and framework upper lower guideway 21.Described upper hydraulic cylinder 3 is arranged on upper cover plate 52 from left to right；Longitudinal hydraulic cylinder 18 is arranged on upper cover plate 52 from the top down；Pipettor hydraulic cylinder 24 is arranged on pipettor mobile vehicle 20；Middle hydraulic cylinder 45 is arranged on the position below the crossbeam of slide glass folder mounting sheet 46 near left side from left to right；Described slide glass folder 53 is arranged on the left side of cylindrical carrier 29 on the crossbeam of slide glass folder mounting sheet 46；20 slide glasses press from both sides 53 in the same size, coincide with the reserved installation position of slide glass folder mounting sheet 46, all slide glasses folder 53 with equidirectional in wire neat horizontal arranged in parallel；Each hydraulic cylinder described is respectively mounted independent displacement transducer.
Described slide glass folder 53, is made up of colourless transparent plastic, upper slice 54 be sieve-like, bottom sheet 55 only has a liquid outlet 56 at edge, and this liquid outlet communicates with upper slice gap between 54 with bottom sheet 55, upper slice carrying tissue slice 57 between 54 and bottom sheet 55.
Described image collecting device, including lower hydraulic cylinder 43, lower frame lower guideway 33, lower frame upper rail 34, digital pathological section scanning system mobile vehicle 36 and digital pathological section scanning system 39.Described lower frame lower guideway 33, lower frame upper rail 34 are arranged on lower cover 31 with direction from left to right, described digital pathological section scanning system mobile vehicle 36 is arranged on lower frame lower guideway 33, lower frame upper rail 34, described digital pathological section scanning system 39 is arranged on digital pathological section scanning system mobile vehicle 36, and lower hydraulic cylinder 43 is arranged on the left side of digital pathological section scanning system mobile vehicle 36.The microscope that described digital pathological section scanning system 39 uses is inverted microscope, it is possible to provide white, red, green and blue four kinds of light sources.
Described master controller obtains the displacement transducer data of each hydraulic cylinder and accurately controls each hydraulic cylinder, and data transmission digital pathological section scanning system 39 obtained processes to PC；Described master controller obtains temperature sensor data and realizes the control to sample environment temperature by regulation heater 32.Described heater 32 is arranged on lower cover 31, and temperature sensor is arranged in framework, makes the temperature in framework control at 37 DEG C；The operating ambient temperature of described a kind of immunostaining machine is room temperature.
Claims (3)
1. an immunostaining machine, it is characterised in that: mainly it is made up of framework and support means, immunostaining device, image collecting device, heater and the several part of master controller；
Described framework and support means, by four big frames, bolt is installed and corresponding big frame is installed nut and is installed together by upper cover plate, lower cover, left cover, right cover plate, front shroud, back shroud and slide glass folder mounting sheet, formed and be divided into upper and lower two-part rectangular box-like structure with slide glass folder mounting sheet；
nullDescribed immunostaining device，Including micropipettor、Pipettor moves carrier、Upper hydraulic cylinder、Longitudinal hydraulic cylinder、Pipettor hydraulic cylinder、Middle hydraulic cylinder、Lower hydraulic cylinder、Pipette slide glass、Slide glass folder and vacuum pump、Framework upper upper rail and framework upper lower guideway and cylindrical carrier，Cylindrical carrier is arranged on position on the right side above slide glass folder mounting sheet crossbeam，Described pipettor moves carrier and is arranged on framework upper upper rail and framework upper lower guideway，Described upper hydraulic cylinder is arranged on upper cover plate from left to right，Longitudinal hydraulic cylinder is arranged on upper cover plate from the top down，Pipettor hydraulic cylinder is arranged on mobile vehicle，Middle hydraulic cylinder is arranged on the position below the crossbeam of slide glass folder mounting sheet near left side from left to right，Described slide glass folder is arranged on the left side of cylindrical carrier on slide glass folder mounting sheet，All slide glasses folder with equidirectional in wire neat horizontal arranged in parallel，Each hydraulic cylinder described is respectively mounted independent displacement transducer；
Described image collecting device, including lower hydraulic cylinder, lower frame lower guideway, lower frame upper rail, digital pathological section scanning system mobile vehicle and digital pathological section scanning system, described lower frame lower guideway, lower frame upper rail is arranged on lower cover with direction from left to right, described digital pathological section scanning system mobile vehicle is arranged on lower frame lower guideway and lower frame upper rail, described digital pathological section scanning system is arranged on digital pathological section scanning system mobile vehicle, described lower hydraulic cylinder is arranged on the left side of digital pathological section scanning system mobile vehicle；
Described master controller obtains displacement transducer data each hydraulic cylinder of accuracy controlling of each hydraulic cylinder, obtains the data of digital pathological section scanning system collection and transmits to PC, obtains temperature sensor data and regulate and control heater.
A kind of immunostaining machine the most according to claim 1, it is characterised in that: described slide glass folder is made up of colourless transparent plastic, upper slice be sieve-like, there is at least one liquid outlet at the edge of bottom sheet, and this liquid outlet communicates with the gap between bottom sheet with upper slice.
A kind of immunostaining machine the most according to claim 1, it is characterised in that: the microscope that described digital pathological section scanning system is used is inverted microscope, it is possible to provide at least white, red, green and blue four kinds of light sources.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620126863.8U CN205483748U (en) | 2016-02-18 | 2016-02-18 | Immunostaining machine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620126863.8U CN205483748U (en) | 2016-02-18 | 2016-02-18 | Immunostaining machine |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205483748U true CN205483748U (en) | 2016-08-17 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620126863.8U Expired - Fee Related CN205483748U (en) | 2016-02-18 | 2016-02-18 | Immunostaining machine |
Country Status (1)
| Country | Link |
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| CN (1) | CN205483748U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105547794A (en) * | 2016-02-18 | 2016-05-04 | 南昌德漫多科技有限公司 | Immune staining machine |
| CN108117972A (en) * | 2017-12-30 | 2018-06-05 | 郑州游爱网络技术有限公司 | A kind of plasma pigment inhibits reaction unit |
-
2016
- 2016-02-18 CN CN201620126863.8U patent/CN205483748U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105547794A (en) * | 2016-02-18 | 2016-05-04 | 南昌德漫多科技有限公司 | Immune staining machine |
| CN105547794B (en) * | 2016-02-18 | 2018-12-04 | 南昌德漫多科技有限公司 | A kind of immunostaining machine |
| CN108117972A (en) * | 2017-12-30 | 2018-06-05 | 郑州游爱网络技术有限公司 | A kind of plasma pigment inhibits reaction unit |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160817 Termination date: 20180218 |