CN105547794B - A kind of immunostaining machine - Google Patents
A kind of immunostaining machine Download PDFInfo
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- CN105547794B CN105547794B CN201610090715.XA CN201610090715A CN105547794B CN 105547794 B CN105547794 B CN 105547794B CN 201610090715 A CN201610090715 A CN 201610090715A CN 105547794 B CN105547794 B CN 105547794B
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- hydraulic cylinder
- slide glass
- pathological section
- pipettor
- scanning system
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- 238000012744 immunostaining Methods 0.000 title claims abstract description 26
- 238000010438 heat treatment Methods 0.000 claims abstract description 10
- 239000011521 glass Substances 0.000 claims description 63
- 230000001575 pathological effect Effects 0.000 claims description 32
- 239000007788 liquid Substances 0.000 claims description 19
- 239000012530 fluid Substances 0.000 claims description 13
- 238000006073 displacement reaction Methods 0.000 claims description 10
- 238000009434 installation Methods 0.000 claims description 7
- 229920003023 plastic Polymers 0.000 claims description 3
- 230000001276 controlling effect Effects 0.000 claims 1
- 230000001105 regulatory effect Effects 0.000 claims 1
- 230000030741 antigen processing and presentation Effects 0.000 abstract description 2
- 230000009286 beneficial effect Effects 0.000 abstract 1
- 238000000034 method Methods 0.000 description 12
- 238000004043 dyeing Methods 0.000 description 10
- 239000000427 antigen Substances 0.000 description 9
- 102000036639 antigens Human genes 0.000 description 9
- 108091007433 antigens Proteins 0.000 description 9
- 238000010166 immunofluorescence Methods 0.000 description 3
- 101710098119 Chaperonin GroEL 2 Proteins 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000000684 flow cytometry Methods 0.000 description 2
- 238000005213 imbibition Methods 0.000 description 2
- 239000012188 paraffin wax Substances 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
- 101000894412 Mycolicibacterium paratuberculosis (strain ATCC BAA-968 / K-10) Bacterioferritin Proteins 0.000 description 1
- 101000588258 Taenia solium Paramyosin Proteins 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000004040 coloring Methods 0.000 description 1
- 230000002380 cytological effect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000003053 immunization Effects 0.000 description 1
- 238000002649 immunization Methods 0.000 description 1
- 238000003125 immunofluorescent labeling Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 238000007447 staining method Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The present invention relates to a kind of immunostaining machines comprising: frame and support device, immunostaining device, image collecting device, heating device and control system.The beneficial effect of the present invention compared with prior art is that multiple immunostaining can be carried out to same histotomy, can carry out alternate immunostaining and Image Acquisition to same histotomy, help to study homocellular a variety of antigen presentation situations.
Description
Technical field
The invention belongs to detection device technical fields, and in particular to be to be applied to one of Hospital Pathological Department histotomy
Immunostaining machine.
Background technique
Immunostaining is to detect the expression feelings of specific protein (antigen) using principle of the antigen in conjunction with antibody specificity
Condition is discovery body one of most common method of changes of function under physiology or pathological state, and the most commonly used is immuning tissues
Learn dyeing and immunofluorescence dyeing.During the experiment, it is often necessary to study at least two antigen/proteins in same cell or group
The expression in sample is knitted, so as to clearly their own positioning or coexpression situation.Commonly used method is that streaming is thin at present
Born of the same parents' art, but the cell concentration that this technology needs is larger, and is used for Flow cytometry by fixed and paraffin embedding tissue
It is ineffective, it cannot accurately show various antigens in tissue and the natural distribution and status of cell in vivo.If tissue specimen amount
Seldom (such as cytological samples obtained by fine-needle aspiration) or the interior cell concentration to be detected of tissue are few, then are difficult to carry out Flow cytometry.By
Multiple immunostaining is carried out to study the expression of a variety of antigens to same sample on histotomy, then can solve this problem.Example
Such as, after the immunofluorescence dyeing that antigen A is carried out to histotomy, due to antigen and antibody be combined into and it is unstable non-covalent
In conjunction with, tissue can be put into buffer, it is appropriate to heat, so that it may to make the antibody in conjunction with antigen A and corresponding colour developing
Agent separates, and can carry out the immunofluorescence dyeing of antigen B, also can be carried out antigens c, antigen D using same method
Dyeing.The Multiple immunizations staining technique for histotomy is had already appeared at present, i.e., same histotomy is once dyed,
A variety of antigen presentations are studied, such as a kind of Double-immunofluorescent labeling method based on same Species origin first antibody
It (CN201410099873.2), is that in the specific implementation process, the antigen for needing to be marked is often not only the shortcomings that this method
Be only two kinds, be 5-10 kind in most cases, or even up to more than 20, this multiple staining method be cannot meet the needs, need into
Row repeatedly dyeing.Certainly, multiple staining can be combined with multiple colouring method, working efficiency can be improved.In addition, multiple
In dyeing course, needs to carry out histotomy to take pictures after dyeing every time, multiple stained photographs are compared, can just be understood fully
Which antigen the same cell of Chu expresses actually, thus, provided device will be also convenient for taking pictures under the microscope.Applicant
A kind of slide glass folder (201520587855.9) for carrying out multiple immunostaining to histotomy is had disclosed, provides one kind just
It is pressed from both sides in the slide glass for carrying out multiple immunostaining to histotomy of craft or machine full-automatic operation, in order to overcome craft
The operation bring amount of labour increases and is not easy the deficiencies of standardizing, it is necessary to provide that one kind has multiple immunostaining and image is adopted
Collect the full-automatic immunostainer of function.
Summary of the invention
The purpose of the present invention is to provide a kind of immunostaining machine, technical problem to be solved is: using existing
Immunostainer can not carry out multiple immunostaining to same histotomy, and same histotomy alternately can not be immunized
Dyeing and Image Acquisition.
In order to solve the above technical problems, the present invention provides a kind of immunostaining machine comprising: frame and support device are exempted from
Epidemic disease dyeing apparatus, image collecting device, heating device and control system.
The frame and support device install nut for upper cover by four big frame installation bolts and corresponding big frame
Plate, lower cover plate, left cover, right cover plate, front shroud, back shroud and slide glass folder mounting sheet are installed together, and are formed to press from both sides with slide glass and be pacified
It puts plate and is divided into two-part rectangular box-like structure up and down.The slide glass folder mounting sheet is in " H " shape, including a crossbeam and two
Arm.
The immunostaining device, including the mobile carrier of micropipettor, pipettor, upper hydraulic cylinder, longitudinal hydraulic cylinder, shifting
On liquid device hydraulic cylinder, middle hydraulic cylinder, lower hydraulic cylinder, pipette slide glass, slide glass folder and vacuum pump, framework upper upper rail and frame
Subordinate's guide rail and cylindrical carrier.Cylindrical carrier is mounted on the position above slide glass folder mounting sheet crossbeam on the right side, carries for storing
Liquid.The window for opening a bar shaped with direction from left to right on upper cover plate, for installing the mobile carrier of pipettor in framework upper
On upper rail and framework upper lower guideway.The upper hydraulic cylinder is mounted on upper cover plate from left to right, for pushing pipettor to move
Dynamic load frame left and right displacement；Longitudinal hydraulic cylinder is mounted on upper cover plate from the top down, the next on pipettor mobile vehicle for pushing
It moves；Pipettor hydraulic cylinder is mounted on mobile vehicle, and micropipettor can be pushed to take liquid push rod, realize micropipettor take liquid and
Blow liquid function；Middle hydraulic cylinder is mounted below the crossbeam of slide glass folder mounting sheet from left to right close to the position in left side, for pushing
Underface and corresponding reset of the pipette slide glass to the placed slide glass folder of slide glass folder mounting sheet.The slide glass folder is mounted on load
Piece presss from both sides the left side of cylindrical carrier on mounting sheet.1-20 slide glass folders are in the same size, the reserved installation position with slide glass folder mounting sheet
It coincide, all slide glass folders are with equidirectional linear neat lateral arranged in parallel.Each hydraulic cylinder is respectively mounted independent displacement
Sensor.
The slide glass folder such as patent (a kind of slide glass folder for carrying out multiple immunostaining to histotomy:
201520587855.9) described, be made of colourless transparent plastic, upper piece be sieve-like, it is more for being carried out to histotomy with one kind
The slide glass folder of secondary immunostaining the difference is that there is at least one fluid hole at the edge of bottom sheet, the fluid hole with upper piece with bottom sheet it
Between gap communicate, sop up the liquid piece between bottom sheet by this hole, upper piece between bottom sheet carrying group
Knit slice.
Described image acquisition device, including lower hydraulic cylinder, lower frame lower guideway, lower frame upper rail, digital pathology
It is sliced scanning system mobile vehicle and digital pathological section scanning system.The lower frame lower guideway, lower frame upper rail
It is mounted on lower cover plate with direction from left to right, the digital pathological section scanning system mobile vehicle is mounted on lower frame
On lower guideway and lower frame upper rail, the digital pathological section scanning system is mounted on the shifting of digital pathological section scanning system
On dynamic load body, the lower hydraulic cylinder is mounted on the left side of digital pathological section scanning system mobile vehicle, for pushing digital disease
Reason slice scanning system reaches slide glass folder position and resets accordingly.The digital pathological section scanning system and traditional number
Word pathological section scanning system the difference is that: used microscope is inverted microscope, it is possible to provide at least white, it is red,
Four kinds of light sources of green and blue.
The master controller obtains the displacement sensor data of each hydraulic cylinder, and real by adjusting corresponding hydraulic cylinder solenoid valve
The now accurate control of each hydraulic cylinder；
The master controller sends instructions to scanner after digital pathological section scanning system is moved to slide glass folder position
Start to scan, the data of scanning are transmitted to PC machine processing, and scanner is moved to down by master controller after the completion of a scan sample
One sample position, and send instruct a scanner again, make it restart to scan.
The master controller obtains temperature sensor data and is realized by adjusting heating device to sample ambient temperature
Control.The heating device is mounted in lower plate, and temperature sensor is mounted in frame, controls the temperature in frame at 37 DEG C
(operating ambient temperature has to be lower than 37 DEG C).
Operation principle and process:
At work, the slide glass for carrying histotomy (paraffin section need to be through being dehydrated) is folded up and is placed in slide glass folder mounting sheet
Predeterminated position, carrier fluid is filled into cylindrical carrier；According to the carrier fluid position of quasi- absorption, upper Driven by Hydraulic Cylinder pipettor
Mobile carrier is displaced micropipettor in left-right direction, after reaching above cylindrical carrier, longitudinal Hydraulic Cylinder pipettor
Mobile vehicle makes micropipettor to bottom offset, declines the suction nozzle of pipettor and reaches carrier fluid, pipettor hydraulic cylinder is pulled upwardly
Micropipettor takes liquid push rod, realizes that micropipettor takes liquid function；Then by longitudinal Driven by Hydraulic Cylinder pipettor mobile vehicle
Micropipettor is shifted up, the suction nozzle of pipettor is made to rise and leave carrier fluid, is carried by upper Hydraulic Cylinder pipettor is mobile
Frame is displaced micropipettor to the right, when the slide glass for being moved to quasi- filling carrier fluid presss from both sides position, longitudinal Hydraulic Cylinder liquid relief
The mobile carrier of device is moved to micropipettor downwards close to slide glass folder, pushes down on micropipettor by pipettor hydraulic cylinder and takes liquid
Liquid is accurately filled on histotomy by push rod.After a certain period of time by setting, middle Hydraulic Cylinder pipette slide glass
The underface pressed from both sides to slide glass, vacuum pump are opened, and the carrier fluid in slide glass folder is sucked, and step that then can be similar carries out a kind of lower load
The filling of liquid.If quasi- carry out Image Acquisition, lower Driven by Hydraulic Cylinder digital pathological section scanning system to slide glass presss from both sides position, and
Driven by Hydraulic Cylinder digital pathological section scanning system resets under after the completion of scanning.After completing Image Acquisition, then carry out next
Step operation.In the whole process, master controller accurately controls corresponding liquid by obtaining the displacement sensor data of each hydraulic cylinder
Cylinder pressure solenoid valve；Accurate control digital pathological section scanning system is moved to slide glass folder position and corresponding scanning process, scanning
Data be transmitted to PC machine processing；It obtains temperature sensor data and is realized by adjusting heating device to sample ambient temperature
Control.
Compared with the prior art, the invention has the advantages that: multiple immunostaining can be carried out to same histotomy；Energy
Alternate immunostaining and Image Acquisition are carried out to same histotomy.
Detailed description of the invention:
Fig. 1 is positive sectional structure chart of the invention；
Fig. 2 is left side view of the invention；
Fig. 3 is right side view of the invention；
Fig. 4 is that slide glass of the invention presss from both sides mounting sheet planform figure；
Fig. 5 is slide glass clamping structure figure of the invention；
Fig. 6 is each hydraulic cylinder mounting means figure of the invention；
Fig. 7 is control structure module schematic diagram of the invention.
In figure: 1, big frame installs bolt；2, big frame installs nut；3, upper hydraulic cylinder；4 ~ 7, upper hydraulic cylinder installs spiral shell
Nail；8, framework upper upper rail；9, framework upper upper rail mounting screw；10 ~ 13, longitudinal hydraulic cylinder mounting screw；14~
17, longitudinal hydraulic cylinder mounting screw；18, longitudinal hydraulic cylinder；19, mobile carrier；20, pipettor mobile vehicle；21, framework upper
Lower guideway；22, big frame installs nut；23, big frame installs bolt；24, pipettor hydraulic cylinder；25, pipettor hydraulic cylinder is pacified
Cartridge screw；26, micropipettor；27, pipettor mounting bar；28, pipettor mounting screw；29, carrier fluid and cylindrical carrier；30,
Right cover plate；31, lower cover plate；32, heating device；33, lower frame lower guideway；34, lower frame upper rail；35, lower frame
Guide rail mounting screw；36, digital pathological section scanning system mobile vehicle；37 ~ 38, digital pathological section scanning system installs spiral shell
Nail；39, digital pathological section scanning system；40, vacuum pump mounting bracket；41, vacuum pump；42, middle hydraulic cylinder leg；43, under
Hydraulic cylinder；44, middle hydraulic cylinder lateral frame；45, middle hydraulic cylinder；46, slide glass presss from both sides mounting sheet；47, imbibition general pipeline；48, middle hydraulic cylinder
Mounting screw；49, pipette slide glass；50, imbibition rubber tube；51, left cover；52, upper cover plate；53, slide glass presss from both sides；54, upper piece；
55, bottom sheet；56, fluid hole；57, histotomy.
Specific embodiment
Embodiments of the present invention are described further with reference to the accompanying drawing:
Frame of the present invention and support device install nut 2 by four big frame installation bolts 1 and corresponding big frame
Upper cover plate 52, lower cover plate 31, left cover 51, right cover plate 30, front shroud, back shroud and slide glass folder mounting sheet 46 are mounted on one
It rises, is formed and two-part rectangular box-like structure up and down is divided into slide glass folder mounting sheet 46.The slide glass presss from both sides mounting sheet 46
" H " shape, including a crossbeam and two arms.
The immunostaining device, including it is the mobile carrier 19 of micropipettor 26, pipettor, upper hydraulic cylinder 3, longitudinal hydraulic
Cylinder 18, pipettor hydraulic cylinder 24, middle hydraulic cylinder 45, lower hydraulic cylinder 43, pipette slide glass 49, slide glass folder 53 and vacuum pump 41, frame
Frame top upper rail 8 and framework upper lower guideway 21 and cylindrical carrier 29.It is horizontal that cylindrical carrier 29 is mounted on slide glass folder mounting sheet 46
Position above beam on the right side.The window for opening a bar shaped with direction from left to right on upper cover plate 52, for installing pipettor
Mobile carrier 19 is on framework upper upper rail 8 and framework upper lower guideway 21.The upper hydraulic cylinder 3 is mounted on from left to right
On cover board 52；Longitudinal hydraulic cylinder 18 is mounted on from the top down on upper cover plate 52；It is mobile that pipettor hydraulic cylinder 24 is mounted on pipettor
On carrier 20；Middle hydraulic cylinder 45 is mounted on the position below the crossbeam of slide glass folder mounting sheet 46 close to left side from left to right；It is described
Slide glass folder 53 is mounted on the left side of cylindrical carrier 29 on the crossbeam of slide glass folder mounting sheet 46；20 slide glass folders 53 are in the same size, with
The reserved installation position that slide glass presss from both sides mounting sheet 46 coincide, and all slide glass folders 53 are with equidirectional linear neat lateral arranged in parallel；
Each hydraulic cylinder is respectively mounted independent displacement sensor.
Slide glass folder 53, is made of colourless transparent plastic, upper piece 54 be sieve-like, bottom sheet 55 only has one to go out liquid at edge
Hole 56, the fluid hole are communicated with upper piece gap between 54 and bottom sheet 55, upper piece histotomy 57 is carried between 54 and bottom sheet 55.
Described image acquisition device, including lower hydraulic cylinder 43, lower frame lower guideway 33, lower frame upper rail 34, number
Word pathological section scanning system mobile vehicle 36 and digital pathological section scanning system 39.The lower frame lower guideway 33, frame
Frame lower part upper rail 34 is mounted on 31 on lower cover plate with direction from left to right, and the digital pathological section scanning system is mobile to be carried
Body 36 is mounted on lower frame lower guideway 33, on lower frame upper rail 34, and the digital pathological section scanning system 39 is installed
On digital pathological section scanning system mobile vehicle 36, lower hydraulic cylinder 43 is mounted on the mobile load of digital pathological section scanning system
The left side of body 36.The microscope that the digital pathological section scanning system 39 uses is inverted microscope, it is possible to provide white, red
Four kinds of color, green and blue light sources.
The master controller obtains the displacement sensor data of each hydraulic cylinder and is accurately controlled each hydraulic cylinder, will
The data that digital pathological section scanning system 39 obtains are transmitted to PC machine processing；The master controller obtains temperature sensor data
And by adjusting control of the realization of heating device 32 to sample ambient temperature.The heating device 32 is mounted on lower cover plate 31,
Temperature sensor is mounted in frame, controls the temperature in frame at 37 DEG C；A kind of working environment of the immunostaining machine
Temperature is room temperature.
Claims (2)
1. a kind of immunostaining machine comprising: frame and support device, immunostaining device, image collecting device, heating device
And control system；
The frame and support device, by four big frames installation bolts and corresponding big frame installation nut by upper cover plate, under
Cover board, left cover, right cover plate, front shroud, back shroud and slide glass folder mounting sheet are installed together, and are formed with slide glass folder mounting sheet point
It is divided into two-part rectangular box-like structure up and down；
The immunostaining device, including the mobile carrier of micropipettor, pipettor, upper hydraulic cylinder, longitudinal hydraulic cylinder, pipettor
Under hydraulic cylinder, middle hydraulic cylinder, lower hydraulic cylinder, pipette slide glass, slide glass folder and vacuum pump, framework upper upper rail and framework upper
Guide rail and cylindrical carrier, cylindrical carrier is mounted on the position above slide glass folder mounting sheet crossbeam on the right side, for storing carrier fluid；On
The window of a bar shaped is provided on cover board with direction from left to right, for installing the mobile carrier of pipettor in framework upper upper rail
On framework upper lower guideway；The upper hydraulic cylinder is mounted on upper cover plate from left to right, for pushing the mobile carrier of pipettor
Left and right displacement；Longitudinal hydraulic cylinder is mounted on upper cover plate from the top down, for pushing pipettor mobile vehicle upper and lower displacement；Liquid relief
Device hydraulic cylinder is mounted on pipettor mobile vehicle, for pushing micropipettor to take liquid push rod, realizes that micropipettor takes liquid
And blow liquid function；Middle hydraulic cylinder is mounted below the crossbeam of slide glass folder mounting sheet from left to right close to the position in left side, for pushing away
Underface and corresponding reset of the dynamic pipette slide glass to the placed slide glass folder of slide glass folder mounting sheet；The slide glass folder is mounted on
Slide glass presss from both sides the left side of cylindrical carrier on mounting sheet；Each slide glass folder is in the same size, kisses with the reserved installation position of slide glass folder mounting sheet
It closes, all slide glass folders are with equidirectional linear neat lateral arranged in parallel；Each hydraulic cylinder is respectively mounted independent displacement and passes
Sensor；
Described image acquisition device, including lower hydraulic cylinder, lower frame lower guideway, lower frame upper rail, digital pathological section
Scanning system mobile vehicle and digital pathological section scanning system, the lower frame lower guideway, lower frame upper rail with from
The direction of from left to right is mounted on lower cover plate, and the digital pathological section scanning system mobile vehicle is mounted under lower frame and leads
On rail and lower frame upper rail, the digital pathological section scanning system is mounted on the mobile load of digital pathological section scanning system
On body, the lower hydraulic cylinder is mounted on the left side of digital pathological section scanning system mobile vehicle；
Master controller obtains the displacement sensor data and each hydraulic cylinder of accuracy controlling of each hydraulic cylinder, obtains digital pathological section and sweeps
It retouches the data of system acquisition and is transmitted to PC machine, obtain temperature sensor data and heating device is regulated and controled；
Slide glass folder is made of colourless transparent plastic, upper piece be sieve-like, there is at least one fluid hole at the edge of bottom sheet, should go out liquid
Hole is communicated with upper piece gap between bottom sheet.
2. a kind of immunostaining machine according to claim 1, it is characterised in that: the digital pathological section scanning system institute
The microscope used is inverted microscope, it is possible to provide at least white, red, four kinds of light sources of green and blue.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610090715.XA CN105547794B (en) | 2016-02-18 | 2016-02-18 | A kind of immunostaining machine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610090715.XA CN105547794B (en) | 2016-02-18 | 2016-02-18 | A kind of immunostaining machine |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105547794A CN105547794A (en) | 2016-05-04 |
| CN105547794B true CN105547794B (en) | 2018-12-04 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610090715.XA Expired - Fee Related CN105547794B (en) | 2016-02-18 | 2016-02-18 | A kind of immunostaining machine |
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Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106289923A (en) * | 2016-09-29 | 2017-01-04 | 南昌德漫多科技有限公司 | A kind of device for same tissue slice being carried out repeatedly immunostaining |
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| CN101021455A (en) * | 2007-03-22 | 2007-08-22 | 广州安必平医药科技有限公司 | Slide-making staining machine and method for slide-making staining method |
| CN102116771A (en) * | 2010-01-04 | 2011-07-06 | 深圳市亚辉龙生物科技有限公司 | Enzyme-linked immune analysis method and fully-automatic enzyme-linked immune analyzer |
| CN102147405A (en) * | 2011-01-12 | 2011-08-10 | 深圳市亚辉龙生物科技有限公司 | Fully automatic biochemical immune analyzer |
| CN104502171A (en) * | 2014-11-27 | 2015-04-08 | 嘉兴凯实生物科技有限公司 | Automatic fluorescence slice dyeing machine |
| CN205483748U (en) * | 2016-02-18 | 2016-08-17 | 南昌德漫多科技有限公司 | Immunostaining machine |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8962258B2 (en) * | 2008-08-29 | 2015-02-24 | Korea Advanced Institute Of Science And Technology | Apparatus and method for multiple immunoassays on a chip |
| JP5629850B2 (en) * | 2010-07-02 | 2014-11-26 | 国立大学法人秋田大学 | Immune tissue staining method and immune tissue staining apparatus |
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2016
- 2016-02-18 CN CN201610090715.XA patent/CN105547794B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101021455A (en) * | 2007-03-22 | 2007-08-22 | 广州安必平医药科技有限公司 | Slide-making staining machine and method for slide-making staining method |
| CN102116771A (en) * | 2010-01-04 | 2011-07-06 | 深圳市亚辉龙生物科技有限公司 | Enzyme-linked immune analysis method and fully-automatic enzyme-linked immune analyzer |
| CN102147405A (en) * | 2011-01-12 | 2011-08-10 | 深圳市亚辉龙生物科技有限公司 | Fully automatic biochemical immune analyzer |
| CN104502171A (en) * | 2014-11-27 | 2015-04-08 | 嘉兴凯实生物科技有限公司 | Automatic fluorescence slice dyeing machine |
| CN205483748U (en) * | 2016-02-18 | 2016-08-17 | 南昌德漫多科技有限公司 | Immunostaining machine |
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