CN106289923A - A kind of device for same tissue slice being carried out repeatedly immunostaining - Google Patents
A kind of device for same tissue slice being carried out repeatedly immunostaining Download PDFInfo
- Publication number
- CN106289923A CN106289923A CN201610863358.6A CN201610863358A CN106289923A CN 106289923 A CN106289923 A CN 106289923A CN 201610863358 A CN201610863358 A CN 201610863358A CN 106289923 A CN106289923 A CN 106289923A
- Authority
- CN
- China
- Prior art keywords
- tissue slice
- groove
- coverslip
- immunostaining
- same tissue
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000012744 immunostaining Methods 0.000 title claims abstract description 17
- 239000011521 glass Substances 0.000 claims abstract description 13
- 239000007788 liquid Substances 0.000 claims description 11
- 239000000203 mixture Substances 0.000 claims 1
- 238000003745 diagnosis Methods 0.000 abstract description 2
- 238000000034 method Methods 0.000 description 12
- 238000004043 dyeing Methods 0.000 description 10
- 239000012128 staining reagent Substances 0.000 description 6
- 239000000427 antigen Substances 0.000 description 4
- 102000036639 antigens Human genes 0.000 description 4
- 108091007433 antigens Proteins 0.000 description 4
- KDXKERNSBIXSRK-RXMQYKEDSA-N D-lysine Chemical compound NCCCC[C@@H](N)C(O)=O KDXKERNSBIXSRK-RXMQYKEDSA-N 0.000 description 2
- 238000004040 coloring Methods 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
- 230000030741 antigen processing and presentation Effects 0.000 description 1
- 230000000890 antigenic effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000003053 immunization Effects 0.000 description 1
- 238000002649 immunization Methods 0.000 description 1
- 238000010166 immunofluorescence Methods 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000012780 transparent material Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
- G01N1/312—Apparatus therefor for samples mounted on planar substrates
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
In order to realize that same tissue slice is carried out repeatedly immunostaining, to save tissue specimen and to meet diagnosis needs, the present invention discloses a kind of device for same tissue slice carries out repeatedly immunostaining, it is characterised in that be made up of slide glass groove and coverslip；Described slide glass groove, has upper groove and the low groove of mutually continuity, and the size and shape of described upper groove matches with coverslip；Described low groove is positioned at upper groove bottom center, bottom flat；The downward one side of described coverslip attaches tissue slice, the space that described tissue slice is positioned at coverslip and low groove surrounds.This device is used to contribute to realizing same tissue slice carrying out immunostaining repeatedly and taking pictures.
Description
Technical field
The present invention relates to medical biotechnology experimental provision, particularly relating to a kind of for same tissue slice being carried out repeatedly immunity
The device of dyeing.
Background technology
Immunostaining is the principle utilizing antigen to be combined with antibody specificity, the expression feelings of detection specific protein (antigen)
Condition, is to find one of body detection method that changes of function is the most frequently used under physiology or pathological state, is most commonly used that immune group
Weave chemistry dyeing and immunofluorescence dyeing.Conventional method is that a tissue slice carries out the colouring method of a kind of antigenic mark,
The expression studying multiple antigen then needs multiple tissue slices.If to minimal amount of tissue specimen (such as fine needle aspiration
Specimen) carry out the research of multiple antigen presentation situation, routine immunization colouring method is owing to being difficult to by specimen quantitative limitation.
Therefore, it is necessary to develop a kind of apparatus and method that same tissue slice can be carried out repeatedly immunostaining, to save tissue mark
This, meet diagnosis needs.
Summary of the invention
The technical problem to be solved is difficult to carry out same tissue slice repeatedly under existence conditions
Immunostaining and taking pictures.
The present invention solves above-mentioned technical problem and the technical scheme is that offer is a kind of for carrying out same tissue slice
The repeatedly device of immunostaining.
A kind of device for same tissue slice being carried out repeatedly immunostaining, it is characterised in that by slide glass groove and Gai Bo
Sheet forms.
Described slide glass groove, is made up of colourless transparent material, has upper groove and the low groove of mutually continuity, described upper groove
Size and shape matches with coverslip, is preferable over and can just accommodate coverslip；Described low groove is positioned at upper groove bottom center,
Bottom flat, volume is 50 ~ 200 microlitres, and low groove cross-sectional area is preferable over slightly larger than tissue slice area, it is ensured that tissue slice
It is entirely located in low groove.Near side side-walls, there is a liquid feeding breach, make the staining reagent of filling enter recessed smoothly
Groove.In close opposite side sidewall, there is a liquid outlet, start from the bottom of low groove, outwards pass from sidewall, for being inhaled by negative pressure
It is directed toward except the staining reagent in low groove.Described coverslip is commercially available coverslip, and feature is: during use, in order to increase tissue
Section and the adhesiveness of coverslip, first clean and process coverslip with smearing the methods such as poly-D-lysine, then fishing for coverslip
Tissue slice, make tissue slice directly against attached on the cover slip, after completing roasting sheet, with tissue slice downwardly direction, by lid glass
Sheet is positioned in upper groove, makes described tissue slice be positioned at coverslip and space that low groove surrounds, complete dewaxing, aquation,
After a series of conventional steps such as antigen retrieval, dyeing that tissue slice is circulated successively, take pictures, clean, dye again, clap again
According to, the repeatedly dyeing cleaned again.In this process, each staining procedure is first from liquid feeding breach filling required dyeing examination
Agent, at tissue slice after process after a while, removes staining reagent from liquid outlet with vacuum extractor.In whole dye
During color, it is not necessary to started by coverslip, staining reagent can fully act on tissue slice；When taking pictures, directly by institute
State a kind of device for same tissue slice carries out repeatedly immunostaining to be placed under microscope, can complete.
Present invention have an advantage that and 1. tissue slice is attached on coverslip, be placed in low groove, it is ensured that dye
Color reagent fully acts on tissue slice；2. avoid in dyeing course start coverslip cause tissue slice to be impacted and
Separate；3. it is easy to dyeing repeatedly and takes pictures.
Accompanying drawing explanation
Fig. 1 is the axis side view of a kind of device for same tissue slice carries out repeatedly immunostaining.
In figure: 1 is slide glass groove；2 is upper groove；3 is coverslip；4 is tissue slice；5 is low groove；6 is liquid feeding breach；
7 is liquid outlet.
Specific implementation method
The present invention is described in detail with instantiation below in conjunction with the accompanying drawings.
As it is shown in figure 1, a kind of device for same tissue slice being carried out repeatedly immunostaining, by slide glass groove 1 and lid
Slide 3 forms.
Described slide glass groove 1, is made up of colourless transparent glass, has upper groove 2 and low groove 5, the described fovea superior of mutually continuity
The size and shape of groove 2 matches with coverslip 3；Low groove 5 is positioned at upper groove 2 bottom center, the bottom flat of low groove 5,
Volume is 100 microlitres, and low groove 5 cross-sectional area is preferable over slightly larger than tissue slice 4 area.At right side wall, there is one
Liquid feeding breach 6, has a liquid outlet 7 in the sidewall of close left side, starts from the bottom of low groove 5, outwards pass from sidewall.Lid glass
Sheet 3 is commercially available coverslip, during use, first cleans and with smearing poly-D-lysine, tissue slice 4 directly against being attached on coverslip 3,
With tissue slice 4 downwardly direction, coverslip 3 is positioned in upper groove 2, make tissue slice 4 be placed exactly in coverslip 3 and under
In the space that groove 5 surrounds, then carry out series of steps such as dyeing, take pictures.In this process, each staining procedure is
First fill required staining reagent from liquid feeding breach 6, at tissue slice after process after a while, with vacuum extractor from going out
Staining reagent is removed by fluid apertures 7.In whole dyeing course, it is not necessary to coverslip 3 is started, when taking pictures, directly by described
A kind of device for same tissue slice carries out repeatedly immunostaining is placed under microscope, can complete.
Claims (3)
1. the device being used for same tissue slice carries out repeatedly immunostaining, it is characterised in that by slide glass groove and coverslip
Composition；Described slide glass groove, has upper groove and the low groove of mutually continuity, the size and shape of described upper groove and coverslip phase
Join；Described low groove is positioned at upper groove bottom center, bottom flat.
A kind of device for same tissue slice being carried out repeatedly immunostaining the most according to claim 1, its feature
It is: described slide glass groove is having a liquid feeding breach near side side-walls, in close opposite side sidewall, has a liquid outlet,
Start from the bottom of low groove, outwards pass from sidewall.
A kind of device for same tissue slice being carried out repeatedly immunostaining the most according to claim 1, its feature
It is: the downward one side of described coverslip attaches tissue slice, the sky that described tissue slice is positioned at coverslip and low groove surrounds
In.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610863358.6A CN106289923A (en) | 2016-09-29 | 2016-09-29 | A kind of device for same tissue slice being carried out repeatedly immunostaining |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610863358.6A CN106289923A (en) | 2016-09-29 | 2016-09-29 | A kind of device for same tissue slice being carried out repeatedly immunostaining |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106289923A true CN106289923A (en) | 2017-01-04 |
Family
ID=57716463
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610863358.6A Pending CN106289923A (en) | 2016-09-29 | 2016-09-29 | A kind of device for same tissue slice being carried out repeatedly immunostaining |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106289923A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108871899A (en) * | 2018-07-12 | 2018-11-23 | 泰山医学院 | Microsection manufacture method for histocyte dyeing |
| CN108931412A (en) * | 2017-05-26 | 2018-12-04 | 深圳大学 | Histotomy antigen retrieval device and method |
| CN108956241A (en) * | 2017-05-26 | 2018-12-07 | 深圳大学 | The multiple staining method of organization chip |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103091826A (en) * | 2012-12-21 | 2013-05-08 | 中国人民解放军第三军医大学第三附属医院 | Set of glass slides used for carrying out immunohistochemistry staining and storage to tissue slice |
| CN105021816A (en) * | 2015-08-07 | 2015-11-04 | 南昌德漫多科技有限公司 | Slice carrying clamp used for conducting multiple times of immunostaining on tissue slice |
| CN105547794A (en) * | 2016-02-18 | 2016-05-04 | 南昌德漫多科技有限公司 | Immune staining machine |
| CN105784991A (en) * | 2016-05-25 | 2016-07-20 | 南昌德漫多科技有限公司 | Device and method for repeated immunostaining of same tissue section |
| CN206515120U (en) * | 2016-09-29 | 2017-09-22 | 南昌德漫多科技有限公司 | A kind of device for being used to carry out same histotomy multiple immunostaining |
-
2016
- 2016-09-29 CN CN201610863358.6A patent/CN106289923A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103091826A (en) * | 2012-12-21 | 2013-05-08 | 中国人民解放军第三军医大学第三附属医院 | Set of glass slides used for carrying out immunohistochemistry staining and storage to tissue slice |
| CN105021816A (en) * | 2015-08-07 | 2015-11-04 | 南昌德漫多科技有限公司 | Slice carrying clamp used for conducting multiple times of immunostaining on tissue slice |
| CN105547794A (en) * | 2016-02-18 | 2016-05-04 | 南昌德漫多科技有限公司 | Immune staining machine |
| CN105784991A (en) * | 2016-05-25 | 2016-07-20 | 南昌德漫多科技有限公司 | Device and method for repeated immunostaining of same tissue section |
| CN206515120U (en) * | 2016-09-29 | 2017-09-22 | 南昌德漫多科技有限公司 | A kind of device for being used to carry out same histotomy multiple immunostaining |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108931412A (en) * | 2017-05-26 | 2018-12-04 | 深圳大学 | Histotomy antigen retrieval device and method |
| CN108956241A (en) * | 2017-05-26 | 2018-12-07 | 深圳大学 | The multiple staining method of organization chip |
| CN108931412B (en) * | 2017-05-26 | 2020-12-04 | 深圳大学 | Tissue slice antigen retrieval device and method |
| CN108956241B (en) * | 2017-05-26 | 2021-04-30 | 深圳大学 | Multiple staining method for tissue chip |
| CN108871899A (en) * | 2018-07-12 | 2018-11-23 | 泰山医学院 | Microsection manufacture method for histocyte dyeing |
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| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170104 |