CN209606372U - Serum lipoprotein agarose gel electrophoresis notcher and bracket - Google Patents

Abstract

The utility model relates to a kind of biochemical test instruments, and in particular to a kind of serum lipoprotein agarose gel electrophoresis notcher and bracket.Including index finger pressing handle, notcher ontology and piston, the notcher ontology is hollow structure, notcher ontology bottom end opening formation Ago-Gel liquid receives chamber, and notcher ontology bottom end lower edge is arranged to edge structure, piston is double-piston setting up and down, it is provided with vertical parallel piston rod between double-piston and pressing handle and forms double piston-rod, piston rod upper end is set with auxiliary spring, and the auxiliary spring upper end connect with index finger pressing handle, the top connection of lower end and notcher ontology.The utility model makes loading slot open sample operation and more simplifies, uniform specification of slotting, point sample is more neat, and ribbon area is shown clearly, standardizes the sample-adding of electrophoresis more without with the naked eye estimating reading slotting position.

Description

Serum lipoprotein agarose gel electrophoresis notcher and bracket

Technical field

The utility model relates to a kind of biochemical test instruments, and in particular to a kind of serum lipoprotein agarose gel electrophoresis is opened Slot device and bracket.

Background technique

Electrophoresis refers to charged particle under electric field action to the phenomenon that its institute's electrically charged opposite electrode direction swimming.Utilize band This characteristic of charged particle separates, purifies, identifies amino acid, polypeptide, protein, nucleic acid, enzyme even virus and cell etc. Technology, referred to as electrophoretic techniques.Electrophoretic techniques can be divided into free electrophoresis (no support) and zone electrophoresis (having support), zone electricity Swimming can make support of various types of substances, therefore application is wide, including paper electrophoresis, thin layer electrophoresis, gel electrophoresis, transfer Electrophoresis and capillary high pressure electrophoresis etc..The equipment as needed for electrophoretic techniques be simple and convenient to operate with resolution ratio with higher, As analytical technology common in medical test.

Serum lipoprotein is a kind of water-soluble compound of high molecular weight, commonly uses supercentrifugation and electrophoresis Plasma lipoprotein is classified.Expensive equipment needed for supercentrifugation, time-consuming；Equipment needed for electrophoresis is simple, operation is simple Just quick, it is still used at present in clinical labororatory's biochemical investigation.The separation of serum lipoprotein, the most commonly used is Ago-Gels Electrophoresis, lipoprotein application lipid-soluble dye colour lipid part, and a point band situation, different lipoprotein can be observed in electrophoresis process Quality and surface charge difference, the speed pulled in same electric field is different.Alpha-lipoprotein swimming is most fast, followed by preceding β- Lipoprotein, beta lipoprotein, chylomicron (CM) not swimming, stay in origin (point sample goes out), and the scanning of optical density meter obtains each section The relative scale of lipoprotein.Normal adult's serum lipoprotein electrophoresis may occur in which that three zone are followed successively by β-rouge from cathode to anode Albumen (depth), prebeta-lipoprotein (shallow), alpha-lipoprotein (slightly deeper than prebeta-lipoprotein) at the origin should be chylomicron.Generally Empty stomach blood specimen CM(-), the concentration ratio of remaining each zone is beta lipoprotein >=alpha-lipoprotein > prebeta-lipoprotein, and alpha-lipoprotein is about 30% is accounted for, prebeta-lipoprotein about 15%, beta lipoprotein accounts for 55% or so.

Clinical meaning are as follows:

If 1, prebeta-lipoprotein combines serum triglyceride apparent increase and cholesterol than alpha-lipoprotein normally deeply or omits Height can be set to IV type hyperlipoprotememia.

If 2, beta lipoprotein zone deep dye person more obvious than normal serum obviously increases in combination with serum total cholesterol, is sweet The oily normal person of three esters is II a type;If increasing in conjunction with serum total cholesterol, the slightly higher slightly molten person of preceding β of triglycerides is II b type.

3, result β-and too busy to get away connect together title " the wide β zone " of preceding β-two zone point are solid in conjunction with serum triglyceride and gallbladder Alcohol, which is increased, can be set to III type.

If 4, it is normal or lower and combine serum triglyceride apparent increase that can be set to I to there is β before chylomicron β for origin Type.

Agarose gel electrophoresis separation serum lipoprotein can be divided into agarose gel plate preparation, sample-adding, electrophoresis, fixation, Colour developing, decoloration, result calculate 7 steps, directly analyze electrophoresis result if non-quantitative, specifically:

1, agarose gel plate prepares the glass plate for the 7.5*2.5cm being uniformly laid on horizontal platform after agarose dissolves by heating On (or slide), notcher carefully is put at the 1.5cm of one end, avoids generating bubble, is stored at room temperature to be solidified, carefully removes Notcher forms loading slot.

2, serum 10ul is added in sample-adding micropipettor in loading slot.

3, the agarose plate after sample-adding is put into electrophoresis tank by electrophoresis, and edges at two ends costs the gauze soaked or filter paper (2- 4 layers), according to 8V/cm, 10 minutes.

4, the agarose plate after fixed electrophoresis is put into fixer 20 minutes, is then placed in 80 °C of baking ovens 80 minutes extremely Until drying.

5, dyeing fat red 7B dyes 10 minutes or oil red " O " dyes 18 hours.

6, destainer decolourizes to the colourless rear natural drying of background.

7, observe and record lipoprotein figure.To sweep to obtain each section lipoprotein percentage at opacimeter wavelength 570nm Than.

And agarose gel plate preparation and the formation of loading slot are very crucial during aforesaid operations, but currently on the market Have not seen the notcher of commercialization, in the actual work, Ago-Gel liquid is prepared more person-portions by us in advance, before use Start bed board after being dissolved by heating, every plank uses the horizontal bed board of Ago-Gel liquid about 3ml, then self-control notcher is hung down Disposed upright is inserted into coagulant liquid and the posture is kept not alter an agreement 3-5 minutes at slide 1.5cm, careful after coagulant liquid solidification Notcher is extracted, that is, forms loading slot.

Self-control notcher is to be cut using common glass slide at both ends, and wind white medical proof fabric at present, is avoided The pushing time is by sense of discomfort caused by notcher in use process, but makes notcher specification by oneself and be difficult to unification, the size of loading slot Or position on slide and whether vertical with slide when fluting, will cause point sample is irregular, band distinguish it is unobvious, and These factors can all directly affect electrophoresis result and analysis, and when formation sample cell, notcher be placed in agarose in advance and coagulated Need to extract after keeping same posture etc. to solidify in glue at 1.5cm, among this, operate it is relatively complicated, 1.5cm at visually Also it is not easy to judge, and is easy in grooving process posture in 3-5 minutes and changes or pull, loading slot is caused to deform.As a result, at present Self-control notcher in the formation operation of loading slot there are problems, use is very inconvenient.

Utility model content

The purpose of this utility model is that the agarose gel plate preparation being directed in prior art electrophoresis sample-adding and loading slot Technical problem present in link is formed, and a kind of serum lipoprotein agarose gel electrophoresis notcher and bracket are provided, this is opened Slot device structure is simple, and specification is consistent, easy to operate, and loading slot forms standard unification, highly promotion and implementation.

The utility model solve scheme used by its technical problem first is that: including index finger press handle, notcher ontology And piston, the notcher ontology are hollow structure, notcher ontology bottom end opening formation Ago-Gel liquid receives chamber, And notcher ontology bottom end lower edge is arranged to edge structure, to form the sharp portion of Ago-Gel liquid fluting, piston is upper and lower The double-piston of setting, double-piston can be pulled freely up and down in notcher body cavity, between double-piston and index finger pressing handle It is arranged there are two vertical parallel piston rod formation double piston-rod, end cap is equipped with auxiliary spring on each piston rod, described auxiliary Auxiliary spring upper end and index finger pressing handle connect, the top connection of lower end and notcher ontology.

It based on the above-mentioned technical proposal, further include fixed glass plate device, the fixed glass plate device is horizontally arranged U-shaped for one Slot, the sharp portion equivalent width of the fixed glass plate device slot insied width and notcher ontology bottom end lower edge, can make spine just Portion enters groove body, and the slot end of the fixed glass plate device is set as the calibration point of loading slot slotting position.

Based on the above-mentioned technical proposal, the notcher ontology top closure or it is arranged to opening, the fluting of top closure Device ontology is only provided with piston hole at piston rod suit.

Based on the above-mentioned technical proposal, it is provided with gasket at the suit on piston rod Yu notcher ontology top, gasket is fixed Setting is connect in notcher ontology top, the auxiliary spring upper end with index finger pressing handle, and lower end is connect with gasket.

Based on the above-mentioned technical proposal, the sharp portion material is plastics or poly (methyl methacrylate) plate, and material thickness is less than or equal to 0.3mm。

Based on the above-mentioned technical proposal, the calibration point of the loading slot slotting position is loading slot at the 1.5cm of gel glass plate Groove width is 2cm, and the sharp portion width of the fixed glass plate device slot insied width and notcher ontology bottom end lower edge is 2cm, the slot length of fixed glass plate device are 1.5cm.

The utility model solve scheme used by its technical problem second is that: including notcher and bracket, it is characterized in that: institute It states notcher to include index finger pressing handle, notcher ontology and piston and fix glass plate device, the notcher ontology is Hollow structure, notcher ontology bottom end opening forms the chamber that receives of Ago-Gel liquid, and notcher ontology bottom end lower edge is arranged At edge structure, to form the sharp portion of Ago-Gel liquid fluting, piston is double-piston setting up and down, and double-piston is being slotted It can freely up and down be pulled in device body cavity, there are two vertical parallel piston rods for setting between double-piston and index finger pressing handle Double piston-rod is formed, end cap is equipped with auxiliary spring on each piston rod, and the auxiliary spring upper end and index finger pressing handle connect It connects, the top connection of lower end and notcher ontology；The bracket includes support plate and positioned at support plate two sides symmetrically fixed two A vertical standpipe, aperture forms vertical guide on the opposite medial surface of two vertical standpipes, is set with one in each vertical standpipe A guide link, the guide link lower part are connected with major-minor spring, and major-minor lower spring end is supported in the support plate；It is described Guide link can vertically guide rail slide up and down, and at least be fixedly connected with that there are two the folders that are laterally arranged between two guide links Plate one is fixedly connected with and is equipped with gap with each clamping plate one mating setting clamping plate two between the clamping plate one and clamping plate two, described Gap between clamping plate one and clamping plate two forms the clamping part for clamping notcher；It is additionally provided on the supporting plate for gel The guide rail of glass plate installation, guide rail direction is vertical with vertical standpipe direction, is arranged one in one end end of guide rail Locating clamp plate, distance of the locating clamp plate apart from notcher projection inner edge are set as the calibration distance of loading slot slotting position.

Based on the above-mentioned technical proposal, the guide rail is integrally located in the middle part of support plate, and guide rail is to be fixed at Two limit bases of support plate on both sides of the middle, the width between two limit bases is gel glass plate width, and locating clamp plate is opposite It is independently arranged in two limit bases.

Based on the above-mentioned technical proposal, the guide rail link together with locating clamp plate or be arranged it is into a single integrated structure, Guide rail and locating clamp plate are formed together a U-shaped guide groove for the sliding of gel glass plate level.

Based on the above-mentioned technical proposal, it is provided with gasket at the suit on piston rod Yu notcher ontology top, gasket is fixed Setting is fixedly connected in notcher ontology top, the auxiliary spring upper end with pressing handle, and lower end is fixedly connected with gasket.

Based on the above-mentioned technical proposal, the calibration point of the loading slot slotting position is loading slot at the 1.5cm of gel glass plate Groove width is 2cm, and the sharp portion width of the guide rail width and notcher ontology bottom end lower edge is 2cm, positioning card Distance of the plate apart from notcher projection inner edge is set as 1.5cm.

The utility model has the advantages that the electrophoresis notcher and bracket of the utility model design, coming with can be to loading slot slotting position The caliberating device positioned, such as: it is set in the fixation glass plate device and embodiment 2 designed in the utility model embodiment 1 The locating clamp plate of meter can make uniform specification of slotting, point sample without with the naked eye estimating reading according to calibration distance fluting More neat, ribbon area is shown clearly, standardizes the sample-adding of electrophoresis more.And operation is simpler, does not have in advance by notcher It is placed in Ago-Gel liquid at 1.5cm, and needs to extract after keeping same posture isogel liquid to solidify, the electricity of the utility model Swimming notcher is provided with handle, and piston, fluting cavity and the sharp portion that can cut coagulant liquid are completed by pressing handle The pushing of coagulant liquid is cut, and using the suction force of piston, can automatically siphon away the coagulant liquid cut away and loading slot is made, mark Under the cooperation for determining device, notcher can be easily put in specified slotting position, and position is accurate, the fluting chamber of notcher lower part Body uniform specification, and coagulant liquid can be formed fixed shape, it is fixed-size open sample slot, can be realized each loading slot fluting rule The unification of lattice, moreover, direct carrying handle suction coagulant liquid forms loading slot after well cutting coagulant liquid, therefore there is no because of posture Loading slot problem on deformation caused by changing or being mobile.In addition, the design for the bracket that the utility model extends, makes loading slot open sample Operation more simplifies, and all operations can be realized in a hand, and fluting positioning is more easy, intuitively, not easy to make mistakes, pushes, cuts It cuts, imbibition operation is more stable, standardizes.The notcher and bracket of the utility model can Reusability, and easy to operate, specification It is unified, highly promotion and implementation.

Detailed description of the invention

Fig. 1 is one of the utility model serum lipoprotein agarose gel electrophoresis notcher structural schematic diagram；

Fig. 2 is the utility model serum lipoprotein agarose gel electrophoresis notcher second structural representation；

Fig. 3 is the fixed glass plate apparatus structure schematic diagram of the utility model；

Fig. 4 is the utility model notcher the schematic diagram of the section structure；

Fig. 5 is the sharp portion enlarged structure schematic diagram in Fig. 3；

Fig. 6 is the structural schematic diagram that the utility model has serum lipoprotein agarose gel electrophoresis notcher bracket；

Fig. 7 is the vertical guide the schematic diagram of the section structure in Fig. 5.

In figure: 1- index finger presses handle, 2- notcher ontology, 3- piston rod, 4- piston, the auxiliary spring of 5-；6- gasket, 7- are solidifying Glue glass plate, 8- support plate, the vertical standpipe of 9-, 10- limit base；11- clamping plate one, 12- clamping plate two, 13- guide link, the main bullet of 14- Spring, 15- coagulant liquid, 16- locating clamp plate；17- fixes glass plate device, 18- vertical guide, 19- sharp portion.

Specific embodiment

The present invention will be further described with reference to the accompanying drawings and examples, to more fully understand the utility model skill Art scheme.

Embodiment 1: a kind of serum lipoprotein agarose gel electrophoresis notcher, the present embodiment are to solve existing self-control and opening Slot device specification is difficult to unification, designs the problems such as operating difficulties, loading slot point sample is irregular, uses the solidifying of the present embodiment notcher Glue glass plate width is 2cm, and loading slot slotting position is located at gel glass plate from the inside 1.5cm in end.Referring specifically to Fig. 1-Fig. 5, Notcher specifically includes index finger pressing handle 1, notcher ontology 2, piston 4 and fixed glass plate device 17, notcher ontology material Matter can be plastics, poly (methyl methacrylate) plate etc., and notcher ontology is hollow structure, and notcher ontology top is open, notcher ontology Lower edge is rectangular parallelepiped structure, and bottom end open-ended forms the chamber that receives of Ago-Gel liquid, and notcher ontology bottom end lower edge is arranged At edge structure, to form the sharp portion of Ago-Gel liquid fluting, lower edge is about 1.8cm, width 2mm, sharp portion material For plastics or poly (methyl methacrylate) plate, material thickness about 0.3mm or hereinafter, material wants sharp but non-friable.Piston is setting up and down Double-piston, double-piston can pull freely up and down in notcher body cavity, be arranged between double-piston and index finger pressing handle Form double piston-rod there are two vertical parallel piston rod, and each piston rod is through connecting two pistons up and down, double-piston and Piston stroke is more stable when the setting of double piston-rod presses down on handle.End cap is equipped with auxiliary spring on each piston rod 5, gasket 6 is provided at the suit on piston rod Yu notcher ontology top, gasket is fixed at notcher ontology top, institute It states auxiliary spring upper end to connect with index finger pressing handle, lower end is connect with gasket.

Fixation glass plate device in the present embodiment is a horizontal positioned U-type groove, U-type groove width and gel glass plate and is opened The sharp portion equivalent width of slot device ontology lower edge, is 2cm, and sharp portion can be made to enter groove body, the slot of fixed glass plate device just End is arranged at 1.5cm, i.e., slot length is 1.5cm, calibration of the slot end when electrophoresis is loaded as loading slot slotting position Point.

When in use, gel glass plate 7 is put in fixed glass plate device groove, starts to spread after dissolving by heating Ago-Gel Plate, every plank use the horizontal bed board of Ago-Gel liquid about 3ml, after coagulant liquid solidification, then by notcher be vertically arranged in away from From (end of i.e. fixed glass plate device) at slide 1.5cm, it is inserted into gel slab, left hand holds notcher ontology middle section, right hand food Finger first presses down on handle, and the downward compressed spring of handle is pressed into notcher ontology apical position, i.e. notcher lower edge to handle Sharp portion be located exactly at the upper surface of Ago-Gel liquid, further press handle, notcher integrally moves down, sharp portion It pushes across Ago-Gel liquid, and coagulant liquid periphery and other coagulant liquids is separated into disengaging, the coagulant liquid of slotted section to be needed After the removing of periphery, upward carrying handle pulls up piston, then piston pulls up the suction to be formed and receives the coagulant liquid of removing It is intracavitary, that is, form loading slot.The present embodiment no longer needs to the open position for the naked eye determining loading slot, directly to fix glass plate device For scale, slotting position is more accurate, and specification is more unified, and entire grooving operations are simple, standardize, and can form unified fluting program, Operator is required without excessive, operator's grooving process comfort level is high, and loading slot uniform specification specification obtained changes existing Make notcher existing various problems when in use by oneself, such as: notcher is placed in 1.5cm in Ago-Gel liquid in advance Place needs among this to extract after keeping same posture etc. to solidify, operate it is relatively complicated, however in actual operation, operation Person is easy to posture change or mobile in the minute of the 3-5 in grooving process, in turn results in loading slot and deforms, and 1.5cm Slotting position naked eyes be also not easy to judge very much, loading slot slotting position disunity, loading slot is more mixed and disorderly, and irregular, these factors are all It is unobvious to will cause band differentiation, influences final testing result.

Embodiment 2: a kind of bracket with serum lipoprotein agarose gel electrophoresis notcher, including notcher and branch Fixed glass plate device is no longer arranged relative to embodiment 1 for frame, notcher part, and the other structures of notcher are same as Example 1, It no longer repeats herein, relative to embodiment 1, is provided with bracket unlike the present embodiment, notcher is matched as a whole with bracket It closes and uses, be advanced optimizing to embodiment 1, specifically:

With embodiment 1, the gel glass plate width using the present embodiment notcher bracket is 2cm, is located at loading slot fluting Gel glass plate is from the inside 1.5cm in end.Referring to Fig. 6-Fig. 7, the bracket in the present embodiment include support plate 8 and be located at support plate The symmetrical fixed two vertical standpipes 9 in two sides, aperture forms vertical guide 18 on the opposite medial surface of two vertical standpipes, each erects A guide link 13 is set with into standpipe, the guide link lower part is connected with main spring 14, the support of main spring lower end In the support plate；Guide link can vertically guide rail slide up and down, be fixedly connected between two guide links there are two The clamping plate one being laterally arranged, and each clamping plate one one clamping plate two of mating setting, be fixedly connected between the clamping plate one, clamping plate two and There are gap, gap width is the thickness of notcher ontology, and the gap between clamping plate one, clamping plate two is formed for clamping notcher Clamping part, notcher ontology is vertically fixed in clamping part, is additionally provided with leading for the installation of gel glass plate on the supporting plate To track, guide rail is integrally located in the middle part of support plate, and guide rail setting direction is vertical with vertical standpipe direction, guide rail For two limit bases for being fixed at support plate on both sides of the middle, the width between two limit bases is gel glass plate width, For 2cm, one locating clamp plate is set in guide rail one end end, locating clamp plate is independently arranged relative to two limit bases, such as Fig. 6 It is shown, certain clearance distance is retained between locating clamp plate and limit base.The locating clamp plate is apart from notcher projection inner edge Distance is set as the calibration point of loading slot slotting position.That is distance of the locating clamp plate apart from notcher projection inner edge is set as 1.5cm。

The present embodiment is provided with matched bracket, when in use, vertically clamps notcher by clamping plate clamping part first, has Body can first vertically place notcher along clamping plate one, then place clamping plate two, clamping plate one, clamping plate two along notcher outer surface Between corresponding position be provided with fixing threaded hole, after placement, be put into screw along screw hole, inwardly hold out against fixed notcher, along leading The gel glass plate added with Ago-Gel liquid is pushed home into track, locating clamp plate position to be pushed into, in the present embodiment Since notcher is positioned in advance, without two hand operations, a hand.One hand of operator is pressed down on index finger Handle makes handle be displaced downwardly to notcher top, further pushes handle at this time, and guide link vertically guide rail slide downward is led Main spring is moved down and compressed to connecting rod, and then clamping plate, notcher ontology is driven to move down, when notcher is displaced downwardly to Ago-Gel liquid Upper surface further pushes handle, then the sharp portion of notcher lower edge removes the Ago-Gel liquid periphery of fluting, removing Afterwards, upward carrying handle, under piston suction effect, the coagulant liquid of removing is sucked away, and after siphoning away, loading slot is formed, and unclamps hand Handle, under the main spring effect in vertical guide, notcher is integrally popped up with clamping plate and is set back, a sample-adding fluting work Sequence is completed, and is resetted and is waited subsequent processing operation.

Embodiment 3: a kind of bracket with serum lipoprotein agarose gel electrophoresis notcher, the present embodiment content and reality Apply that 2 content of example is essentially identical, and same section content no longer repeats, unlike the first embodiment: the guide rail and positioning card Plate links together or is arranged into a single integrated structure, and guide rail and locating clamp plate are formed together one for gel glass plate level The U-shaped guide groove of sliding.

Claims (10)

1. a kind of serum lipoprotein agarose gel electrophoresis notcher, it is characterized in that: including that index finger presses handle, notcher ontology And piston, the notcher ontology are hollow structure, notcher ontology bottom end opening formation Ago-Gel liquid receives chamber, And notcher ontology bottom end lower edge is arranged to edge structure, to form the sharp portion of Ago-Gel liquid fluting, piston is upper and lower The double-piston of setting, double-piston can be pulled freely up and down in notcher body cavity, between double-piston and index finger pressing handle It is arranged there are two vertical parallel piston rod formation double piston-rod, each piston rod is through two pistons up and down are connected, each Piston rod upper end is set with auxiliary spring, and the auxiliary spring upper end connect with index finger pressing handle, the top of lower end and notcher ontology End connection.

2. serum lipoprotein agarose gel electrophoresis notcher according to claim 1, it is characterized in that: further including fixed glass Panel assembly, the fixed glass plate device are a horizontal positioned U-type groove, and the fixed glass plate device slot insied width is equal to notcher The sharp portion width of ontology bottom end lower edge can make sharp portion enter groove body, the slot end setting of the fixed glass plate device just For the calibration point of loading slot slotting position.

3. serum lipoprotein agarose gel electrophoresis notcher according to claim 1, it is characterized in that: the notcher sheet Body top closure is arranged to opening, and the notcher ontology of top closure is only provided with piston hole at piston rod suit.

4. serum lipoprotein agarose gel electrophoresis notcher according to claim 1, it is characterized in that: piston rod with open Be provided with gasket at the suit on slot device ontology top, gasket is fixed at notcher ontology top, the auxiliary spring upper end with Index finger presses handle connection, and lower end is connect with gasket.

5. serum lipoprotein agarose gel electrophoresis notcher according to claim 1, it is characterized in that: the sharp portion material Matter is plastics or poly (methyl methacrylate) plate, and material thickness is less than or equal to 0.3mm.

6. serum lipoprotein agarose gel electrophoresis notcher according to claim 2, it is characterized in that: the loading slot is opened The calibration point of groove location is at the 1.5cm of gel glass plate, and loading slot groove width is 2cm, the fixed glass plate device slot inner width The sharp portion width of degree and notcher ontology bottom end lower edge is 2cm, and the slot length of fixed glass plate device is 1.5cm.

7. a kind of bracket with serum lipoprotein agarose gel electrophoresis notcher, including notcher and bracket, it is characterized in that: The notcher includes index finger pressing handle, notcher ontology and piston and fixed glass plate device, the notcher ontology For hollow structure, notcher ontology bottom end opening formation Ago-Gel liquid receives chamber, and notcher ontology bottom end lower edge is set It is set to edge structure, to form the sharp portion of Ago-Gel liquid fluting, piston is double-piston setting up and down, and double-piston is being opened It can freely up and down be pulled in slot device body cavity, there are two vertical parallel pistons for setting between double-piston and index finger pressing handle Bar forms double piston-rod, and end cap is equipped with auxiliary spring on each piston rod, the auxiliary spring upper end and index finger press handle connect, Lower end is connect with the top of notcher ontology；The bracket include support plate and positioned at support plate two sides symmetrically fixed two it is perpendicular To standpipe, aperture forms vertical guide on the opposite medial surface of two vertical standpipes, is set with one in each vertical standpipe and leads To connecting rod, the guide link lower part is connected with main spring, and main spring lower end is supported in the support plate；The guide link It can vertically guide rail slide up and down, be at least fixedly connected between two guide links there are two the clamping plate one that is laterally arranged, with Each clamping plate one mating setting clamping plate two is fixedly connected between the clamping plate one and clamping plate two and is equipped with gap, the clamping plate one Gap between clamping plate two forms the clamping part for clamping notcher；It is additionally provided with and pacifies for gel glass plate on the supporting plate The guide rail of dress, guide rail direction is vertical with vertical standpipe direction, and a positioning card is arranged in one end end of guide rail Plate, distance of the locating clamp plate apart from notcher projection inner edge are set as the calibration distance of loading slot slotting position.

8. the bracket according to claim 6 with serum lipoprotein agarose gel electrophoresis notcher, it is characterized in that: institute Guide rail to be stated to be integrally located in the middle part of support plate, guide rail is two limit bases for being fixed at support plate on both sides of the middle, Width between two limit bases is gel glass plate width, and locating clamp plate is independently arranged relative to two limit bases.

9. the bracket according to claim 6 with serum lipoprotein agarose gel electrophoresis notcher, it is characterized in that: institute It states guide rail to link together with locating clamp plate or be arranged into a single integrated structure, guide rail and locating clamp plate are formed together one A U-shaped guide groove for the sliding of gel glass plate level.

10. according to the described in any item brackets with serum lipoprotein agarose gel electrophoresis notcher of claim 7-9, Be characterized in: the calibration point of the loading slot slotting position is at the 1.5cm of gel glass plate, and loading slot groove width is 2cm, described The sharp portion width of guide rail width and notcher ontology bottom end lower edge is 2cm, and locating clamp plate is in notcher projection The distance on side is set as 1.5cm.