CN203144413U

CN203144413U - PH (potential of hydrogen) value test comparison device for mammal cell culture fluid

Info

Publication number: CN203144413U
Application number: CN 201320166799
Authority: CN
Inventors: 刘湘涛; 王光祥; 尚佑军
Original assignee: Lanzhou Veterinary Research Institute of CAAS
Current assignee: Lanzhou Veterinary Research Institute of CAAS
Priority date: 2013-04-03
Filing date: 2013-04-03
Publication date: 2013-08-21
Anticipated expiration: 2023-04-03

Abstract

The utility model provides a PH (potential of hydrogen) value test comparison device for mammal cell culture fluid. The PH value test comparison device comprises a support, a colorimetric cup and 14 colorimetric plates, wherein the support is a box body; the colorimetric plates can be accommodated in the box body after being superposed; each colorimetric plate comprises a strip-shaped basal plate prepared by a pure white resin material and 9 rectangular standard color cards pasted on the basal plate in the length direction; each standard color card represents a PH value of the corresponding culture fluid, and the standard color cards respectively represent the colors of the culture fluid with the PH values of 6.4, 6.6, 6.8, 7.0, 7.2, 7.4, 7.6, 7.8 and 8.0 from shallow to deep; the colorimetric cup is a cuboid container with uniform thickness; and the bottom surface and three side surfaces are all prepared by opaque pure white resin materials and the remaining side surface is prepared by a transparent resin material. In the test process, the prepared culture fluid is added to the colorimetric cup, and then the color shown by the culture fluid in the colorimetric cup is compared with the color of each color card of the colorimetric plate to find out the color card proximal to the color and read the PH value.

Description

Mammalian cell nutrient solution pH value test comparison device

Technical field

The utility model relates in the mammalian cell culturing process, and pH value test comparison device belongs to the biological field category.

Background technology

In recent years, the animal cell culture technology has been widely used in every field such as pharmaceutical grade protein research and development, vaccine manufacturing, stem cell transplantation, artificial tissue organ culture, becomes the powerful tools of current life science and biological medicine exploitation day by day.Because the acellular wall of zooblast, accommodative ability of environment is poor, requires harsh to growing environment and culture environment.Every kind of cell has its optimum pH, and various cells are also incomplete same to the requirement of PH, and the optimum pH scope that departs from the cell growth will produce injurious effects to cellular metabolism and growth, cause apoptosis to speed up, even dead.

Most of mammalian cell nutrient solutions use phenol red as the PH indicator.Red expression neutral PH, the acid PH of yellow representative, purple is represented alkaline PH.Measure the most frequently used method of cell culture fluid pH value at present PH meter and two kinds of methods of test paper are arranged, PH meter needs power supply because volume is bigger, and is very inconvenient aseptic, super clean bench operation, popping one's head in simultaneously, it is aseptic to accomplish, causes nutrient solution to pollute when test cell nutrient solution pH value easily.Though the PH test strip is easy to use, measuring accuracy is very poor, and general pH value gap just can have obvious color to distinguish between 0.5 to 1.Most of laboratories lean on experimenter's test experience visual inspection at ordinary times to judge when culturing cell, and are definite very inaccurate to the PH of nutrient solution like this, and very big subjectivity is arranged.Often occur because the cell culture fluid pH value is not adjusted to the required best pH value of cell growth, and make the cell growth not good, in addition dead.Like this scientific research and exploitation are caused very big waste.

Summary of the invention

The purpose of this utility model is in order to overcome above-mentioned shortcoming, to provide a kind of with low cost, simple to operate, being convenient to the comparison device of laboratory convenient, fast, accurate test cell nutrient solution pH value.

For achieving the above object, the technical solution adopted in the utility model is:

A kind of mammalian cell nutrient solution pH value test comparison device is characterized in that: comprise support, cuvette and 14 color boards, wherein:

Described support is a box body, can be accommodated in this box body after described color board is stacked;

Each piece color board includes pure white resin material the bar shaped substrate of making and 9 rectangle standard colour cards pasting along its length on substrate, every standard colour card represents the pH value of corresponding nutrient solution, and it is 6.4,6.6,6.8,7.0,7.2,7.4,7.6,7.8,8.0 nutrient solution color that described 9 rectangle standard colour cards represent pH value from shallow to deep respectively;

Described cuvette is the uniform cuboid container of wall thickness, and its bottom surface and three sides are opaque pure white resin material and make, and surplus next side is that transparent resin material is made.

Described mammalian cell nutrient solution pH value test comparison device, wherein: described standard colour card is that corresponding nutrient solution is under plain white background and the photochrome of nutrient solution solid colour.

Described mammalian cell nutrient solution pH value test comparison device, wherein: described standard colour card is pasted behind substrate and is also handled through press mold.

Described mammalian cell nutrient solution pH value test comparison device, wherein: described cuvette is the cuboid container of 10mm * 10mm * 50mm.

Described mammalian cell nutrient solution pH value test comparison device, wherein: in described 14 color boards 7 are respectively that RPMI-1640, DMEM, MEM, IMDM, M199, McCoys5A, F10 contain serum cell culture fluid pH value color board, and other 7 is respectively that RPMI-1640, DMEM, MEM, IMDM, M199, McCoys5A, F10 do not contain serum cell culture fluid pH value color board.

Compared with prior art, adopt the advantage that the utlity model has of technique scheme to be: to adopt said apparatus, using method of the present utility model is all very easy, in process of the test, as long as being added cuvette, the liquid for preparing compares with the standard color comparison card that this device mates, just can measure the pH value of this nutrient solution, tolerance range is in 0.2.This device is not only accurate, and quick, simple and easy, and does not exist because of test cultures liquid pH value and make the cell culture fluid pollution problems.Simultaneously for test synchronously can also be according to different nutrient solutions, the different tests requirement, oneself changes color board at any time, makes test result more accurate.Because color board is resin material, after the fixed standard colorimetric card, handle through press mold again, be convenient to cleaning and disinfection, can repeat repeatedly to use.Its three faces of the cuvette of rectangular parallelepiped and the end are pure white opaque resin material, and a face is transparent resin material, and the color of observing under natural light or fluorescent lamp makes the pH value of measuring more accurate more near true colors like this.

Description of drawings

Fig. 1 is the structural representation of the utility model mammalian cell nutrient solution pH value test comparison device;

Fig. 2 is the structural representation of cuvette;

Fig. 3 A, Fig. 3 B are respectively that RPMI-1640 contains serum cell culture fluid pH value color board and the synoptic diagram that does not contain serum cell culture fluid pH value color board;

Fig. 4 A, Fig. 4 B are respectively that DMEM contains serum cell culture fluid pH value color board and the synoptic diagram that does not contain serum cell culture fluid pH value color board;

Fig. 5 A, Fig. 5 B are respectively that MEM contains serum cell culture fluid pH value color board and the synoptic diagram that does not contain serum cell culture fluid pH value color board;

Fig. 6 A, Fig. 6 B are respectively that IMDM contains serum cell culture fluid pH value color board and the synoptic diagram that does not contain serum cell culture fluid pH value color board;

Fig. 7 A, Fig. 7 B are respectively that M199 contains serum cell culture fluid pH value color board and the synoptic diagram that does not contain serum cell culture fluid pH value color board;

Fig. 8 A, Fig. 8 B are respectively that McCoys5A contains serum cell culture fluid pH value color board and the synoptic diagram of increase serum cell culture fluid color board not;

Fig. 9 A, Fig. 9 B are respectively that F10 contains serum cell culture fluid pH value color board and the synoptic diagram that does not contain serum cell culture fluid pH value color board;

Description of reference numerals: 1-support; The 2-color board; The 3-standard colour card; The 4-cuvette.

Embodiment

As shown in Figure 1 and Figure 2, the utility model provides a kind of mammalian cell nutrient solution pH value test comparison device, and it comprises support 1, color board 2 and cuvette 4, wherein:

Described support 1 is the box body of (figure slightly) with cover, can be accommodated in this box body after described color board 2 is stacked.

Described color board 2 has 14, wherein 7 is respectively that RPMI-1640, DMEM, MEM, IMDM, M199, McCoys5A, F10 contain serum cell culture fluid pH value color board, and other 7 is respectively that RPMI-1640, DMEM, MEM, IMDM, M199, McCoys5A, F10 do not contain serum cell culture fluid pH value color board.Each piece color board 2 includes pure white resin material the bar shaped substrate of making and the square standard colour card of pasting along its length on substrate 3 of 9 block lengths, described standard colour card 3 can be the photochrome of corresponding nutrient solution under plain white background, it is pasted behind substrate and also handles through press mold, so that cleaning and disinfection makes color board 2 can repeat repeatedly to use.

Wherein, the color of the square standard colour card 3 of described 9 block lengths is respectively that corresponding nutrient solution is the color of 6.4,6.6,6.8,7.0,7.2,7.4,7.6,7.8,8.0 o'clock nutrient solutions at pH value.

For example shown in Figure 5, the MEM zooblast is the color of the standard colour card 3 corresponding with pH value 7.6 on the increase serum substratum color board 2 not, be exactly MEM not the increase serum cell culture fluid be 7.6 o'clock color at pH value;

Described cuvette 4 is for the wall thickness of 10mm * 10mm * 50mm is the cuboid container of 2mm, and its three sides and bottom surface are pure white opaque resin material and make, and surplus next side is that transparent resin material is made.

Adopt said structure, using method of the present utility model is very easy, in process of the test, color board 2 with correspondence takes out from box body and is fixed on the described support 1 earlier, then the nutrient solution for preparing is added cuvette 4, then the color of each standard colour card 3 is compared in the color that nutrient solution in the color board 2 is showed and the color board 2, finds the immediate standard colour card 3 of color, the interpretation pH value.If cuvette 4 nutrient solution colors are between two standard colour cards, then the number of degrees can be judged to the colour atla intermediate value.

Use the utility model, the pH value measuring accuracy is in 0.2.And the utility model is not only accurate, and quick, simple and easy, and does not exist because of test cultures liquid pH value and make the cell culture fluid pollution problems.Simultaneously, for synchronous with test, can also be according to different nutrient solutions, the different tests requirement, oneself changes color board 2 at any time, makes test result more accurate.Because color board 2 is resin materials, after fixed standard colour atla 3, handle through press mold again, be convenient to cleaning and disinfection, can repeat repeatedly to use.Three sides and the bottom surface of the cuvette 4 of rectangular parallelepiped are opaque pure white resin material, and a side is transparent resin material, and the color of observing under natural light or fluorescent lamp makes the pH value of measuring more accurate more near true colors like this.

More than explanation is just illustrative for the utility model; and nonrestrictive, those of ordinary skills understand, under the situation that does not break away from the spirit and scope that claim limits; can make many modifications, variation or equivalence, but all will fall within the protection domain of the present utility model.

Claims

1. a mammalian cell nutrient solution pH value is tested comparison device, it is characterized in that: comprise support, cuvette and 14 color boards, wherein:

2. mammalian cell nutrient solution pH value according to claim 1 test comparison device is characterized in that: described standard colour card is that corresponding nutrient solution is under plain white background and the photochrome of nutrient solution solid colour.

3. mammalian cell nutrient solution pH value according to claim 2 is tested comparison device, it is characterized in that: described standard colour card is pasted behind substrate and is also handled through press mold.

4. mammalian cell nutrient solution pH value according to claim 1 is tested comparison device, and it is characterized in that: described cuvette is the cuboid container of 10mm * 10mm * 50mm.

5. mammalian cell nutrient solution pH value according to claim 1 is tested comparison device, it is characterized in that: in described 14 color boards 7 are respectively that RPMI-1640, DMEM, MEM, IMDM, M199, McCoys5A, F10 contain serum cell culture fluid pH value color board, and other 7 is respectively that RPMI-1640, DMEM, MEM, IMDM, M199, McCoys5A, F10 do not contain serum cell culture fluid pH value color board.