CN202131306U

CN202131306U - Multifunctional microculture system

Info

Publication number: CN202131306U
Application number: CN 201120180143
Authority: CN
Inventors: 吴晓春
Original assignee: 吴晓春
Current assignee: Guangzhou Yoder Biological Technology Co., Ltd.
Priority date: 2011-05-31
Filing date: 2011-05-31
Publication date: 2012-02-01
Anticipated expiration: 2021-05-31

Abstract

The utility model discloses a multifunctional microculture system. The multifunctional microculture system comprises a control circuit assembly and a culture tank, wherein the culture tank is connected with a vacuum pump and a mixed gas bottle through a pipeline respectively; a pressure sensor is arranged on a connecting pipeline of the culture tank and the mixed gas bottle; actions of the vacuum pump, the mixed gas bottle and the pressure sensor are controlled by the control circuit assembly; and feedback information is displayed by a display screen which is electrically connected with the control circuit assembly. By the multifunctional microculture system, the integral pipeline structure of the conventional anaerobic and microaerophilic gas processing device is simplified, so that gas can be pumped and replaced more easily and quickly, and the anaerobic/ microaerophilic environment in the culture tank can be formed only through a few times of gas pumping and replacement; each operating process can be observed on the display screen at any time, so that precision and real-time control can be realized; the multifunctional microculture system is simple and reliable in structure, can regulate and control air pressure at each position, pump the gas easily and quickly and form various anaerobic/ microaerophilic environments, and can be extensively applied to various microculture experiments.

Description

The multifunctional microbial culture systems

Technical field

The utility model relates to the microorganism culturing system that uses in a kind of microbiology field.

Background technology

When carrying out Experiment on Microbiology, many times all will be with to O ₂And CO ₂The bacterium (anerobes, microaerobe, have a liking for the CO2 bacterium) that demands of different is arranged is as experimental subjects.At this time just need to build an anaerobism or microaerophilic environment, cultivate these bacteriums.Usually; This anaerobism or little aerobic environment are accomplished through anaerobism, little aerobic gas treatment equipment; This gas treatment equipment; Be development from the Maclntosh & Fildes vacuum pump drainage gas displacement method of generally acknowledging, adopt, make and obtain anaerobic or little oxygen environment in the culture tank vacuumizing, fail the step of mixed gas in the culture tank.Yet the anaerobism of this routine, little aerobic gas treatment equipment, complex structure, pipeline is staggered, and often can not monitor the air pressure environment in the culture tank well, the levels of precision that cause bleeding, gas concentration does not reach requirement of experiment in the jar after the gas transmission.Moreover because valve is numerous, can not finely detect when being easy to generate gas leakage, the maintenance difficulty, and pump drainage gas efficient is lower.

The utility model content

The purpose of the utility model, be can regulate and control everywhere air pressure easily in order to provide a kind of, pump drainage gas multifunctional microbial culture systems simply rapidly.

The solution that the utility model solves its technical problem is:

The multifunctional microbial culture systems; It comprises pilot circuit assembly and culture tank; Said culture tank respectively with vacuum pump with mix the gas cylinder pipe connection; Wherein culture tank is provided with pressure transmitter with the connecting tube that mixes gas cylinder, and the action of said vacuum pump, mixing gas cylinder and pressure transmitter is controlled by the pilot circuit assembly, and feedback information is shown by the image display that is electrically connected with the pilot circuit assembly.

As the further improvement of technique scheme, the connecting tube of said vacuum pump and culture tank is provided with first SV, and said first SV is electrically connected with the pilot circuit assembly.

Further improvement as technique scheme; Be provided with the 3rd SV and second SV on the connecting tube of said mixing gas cylinder and culture tank in order; Said pressure transmitter is between the 3rd SV and second SV, and the 3rd SV and second SV all are electrically connected with the pilot circuit assembly.

As the further improvement of technique scheme, said pilot circuit assembly is for comprising the pilot circuit of ADC (A/D converter) and CPU (cpu).

As the further improvement of technique scheme, the utility model also comprises the constant incubator that supplies culture tank to insert.

The beneficial effect of the utility model is: the utility model is through setting up vacuum pump, culture tank and mixing gas cylinder; And utilize the action of pressure sensor monitoring pipeline pressure, pilot circuit assembly controller spare; Simplified the integrated piping structure of conventional anaerobism, little aerobic gas treatment equipment; And make the pump drainage substitution gas fast simple more; Only need few number of times can build anaerobism in the culture tank/little aerobic environment, observe, realize accurately, controlled in real-time but be engraved on the image display during each operating process.The utility model is simple and reliable for structure, can regulate and control everywhere air pressure easily, pump drainage gas is simply rapid, makes various anaerobism/little aerobic environment, is widely used in the middle of the various microorganism culturing experiments.

Description of drawings

Below in conjunction with accompanying drawing and embodiment the utility model is further described.

Fig. 1 is that the one-piece construction module of the utility model connects synoptic diagram.

Embodiment

With reference to Fig. 1; The multifunctional microbial culture systems; It comprises pilot circuit assembly 5 and culture tank 1, said culture tank 1 respectively with vacuum pump 2 with mix gas cylinder 3 pipe connection, wherein culture tank 1 is provided with pressure transmitter 4 with the connecting tube that mixes gas cylinder 3; Said vacuum pump 2, mix gas cylinder 3 and control by pilot circuit assembly 5, and feedback information is shown by the image display 6 that is electrically connected with pilot circuit assembly 5 with the action of pressure transmitter 4.Said vacuum pump 2 is accurate oilless vacuum pump, makes pump drainage gas more precisely, fast.Image display 6 is a light-emitting diode display.In actually operating, said culture tank 1 has some kinds of different volumes, forms a series, supplies the not occasion of co-content needs.Before experimentizing, the hydrogen-oxygen catalyzer in the time of can inserting the caesium particle as culturing bacterium in the culture tank 1.

Further as preferred embodiment, said vacuum pump 2 is provided with first SV 11 with the connecting tube of culture tank 1, and said first SV 11 is electrically connected with pilot circuit assembly 5.

Further as preferred embodiment; Be provided with the 3rd SV 31 and second SV 21 in order on the connecting tube of said mixing gas cylinder 3 and culture tank 1; Said pressure transmitter 4 is between the 3rd SV 31 and second SV 21, and the 3rd SV 31 and second SV 21 all are electrically connected with pilot circuit assembly 5.

Further as preferred embodiment, said pilot circuit assembly 5 is for comprising the pilot circuit of ADC (A/D converter) and CPU (cpu).This pilot circuit is controlled the switching of first SV 11, second SV 21 and the 3rd SV 31, and receives the pressure information that pressure transmitter 5 feeds back, and is reflected to immediately on the image display 6.Pilot circuit assembly 5 built-in microprocessing systems itself have configured pump drainage gas, metathetical computer program in a cover Bioexperiment Pretesting, the experiment, can with user's immediate interactive.

Further as preferred embodiment, the utility model also comprises the constant incubator 7 that supplies culture tank 1 to insert.Said constant incubator 7 can reach the stable purpose of nurturing an environment of keeping jar interior mikrobe through the envrionment temperature around the control culture tank 1.

The operation steps of the utility model is such:

One, at first carry out gas tank and connect test, it comprises three steps:

1, vacuum pump 2, first SV 11, the 3rd SV 31 are closed; Second SV 21 is opened, and this moment, the opening of culture tank 1 was controlled by second SV 21, but whether test cultures jar 1 connects like this; Connect whether a jar internal pressure is a normal atmosphere, if then pass through; Otherwise then shut down, the user can carry out connection inspection in kind again.

2, vacuum pump 2, first SV 11, second SV 21 are opened, and the 3rd SV 31 cuts out, and at this moment vacuum pump 2 is communicated with culture tank 1, mixes gas cylinder 3 and does not connect in the gas circuit, and whether this is to be connected correctly with vacuum pump 2 for test cultures jar 1.

3, vacuum pump 2, first SV 11, the 3rd SV 31 are closed, and second SV 21 is opened, and at this moment pressure transmitter 4 detects jar internal pressures, and is correct if culture tank 1 connects, and then passes through; Otherwise then shut down, the user carries out connection inspection in kind.

Two, just carry out gas cylinder afterwards and connect test; Vacuum pump 2, first SV 11, second SV 21 are closed, and the 3rd SV 31 is opened, and whether this just can connect correctly by test mixing gas cylinder 3; And can whether correct through pressure transmitter 5 inspection bottle internal gas pressures, correctly then pass through; Otherwise then shut down and let the inspection of user's essence.

Three, vacuum pump 2, first SV 11, second SV 21 are opened, and the 3rd SV 31 cuts out, and vacuum pump 2 actions at this moment vacuumize culture tank 1.

Four, carry out the gas tank sealing test; Vacuum pump 2, first SV 11, the 3rd SV 31 are closed; Second SV 21 is opened; Pressure transmitter 5 just may detect the pressure condition in the culture tank 1 like this, and pressure test is done in culture tank 1 inside that exhausts after the vacuum, carries out next step gas displacement after qualified.

Five, gas displacement promptly lets vacuum pump 2, first SV 11 close, and second SV 21 and the 3rd SV 31 are opened, and mix gas cylinder 3 and be communicated with culture tank 1 this moment, in culture tank 1, charges into mixed gas.

Six, tank body leak-testing and caesium seed activity test.Make vacuum pump 2, first SV 11, the 3rd SV 31 be in closing condition; Open second SV 21; Have or not leakage in the culture tank 1 after the pressure transmitter 4 inspection inflations; And whether the caesium particle keep active, if culture tank 1 do not have leak and the caesium seed activity enough, then image display 6 shows and passes through; Otherwise do not pass through.

If the utility model is in holding state, then vacuum pump 2, first SV, 11, the second SVs 21 and the 3rd SV 31 all are in closing condition.Whether are the data of each above-mentioned process and next step carrying out, and all can be presented at synchronously in real time on the LED display, and the user can let pilot circuit assembly 5 control the switching of each pump housings, valve through the manipulation operation interface.

The anaerobic environment manufacturing processed, oxygen concentration drops to 0.2% behind three pump drainage replacement operators of the utility model, and surplus oxygen of palladium beaded catalyst catalysis and hydrogen reaction form H ₂O reaches 100% anaerobic state.Little aerobic environment only needs a pump drainage displacement can reach 6% oxygen concn.

More than be that preferred embodiments to the utility model specifies; But the invention is not limited to said embodiment; Those of ordinary skill in the art also can make all equivalent variations or replacement under the prerequisite of the utility model spirit, modification that these are equal to or replacement all are included in the application's claim institute restricted portion.

Claims

1. multifunctional microbial culture systems; It is characterized in that: it comprises pilot circuit assembly (5) and culture tank (1); Said culture tank (1) is respectively with vacuum pump (2) with mix gas cylinder (3) pipe connection; Wherein culture tank (1) is provided with pressure transmitter (4) with the connecting tube that mixes gas cylinder (3); Said vacuum pump (2), mixing gas cylinder (3) are controlled by pilot circuit assembly (5) with the action of pressure transmitter (4), and feedback information is shown by the image display (6) that is electrically connected with pilot circuit assembly (5).

2. multifunctional microbial culture systems according to claim 1; It is characterized in that: said vacuum pump (2) is provided with first SV (11) with the connecting tube of culture tank (1), and said first SV (11) is electrically connected with pilot circuit assembly (5).

3. multifunctional microbial culture systems according to claim 1; It is characterized in that: be provided with the 3rd SV (31) and second SV (21) in order on the connecting tube of said mixing gas cylinder (3) and culture tank (1); Said pressure transmitter (4) is positioned between the 3rd SV (31) and second SV (21), and the 3rd SV (31) and second SV (21) all are electrically connected with pilot circuit assembly (5).

4. according to claim 1,2 or 3 described multifunctional microbial culture systems, it is characterized in that: said pilot circuit assembly (5) is for comprising the pilot circuit of ADC and CPU.

5. multifunctional microbial culture systems according to claim 1 is characterized in that: it also comprises the constant incubator (7) that supplies culture tank (1) to insert.