CN210237634U - Anaerobic culture medium preparation system - Google Patents
Anaerobic culture medium preparation system Download PDFInfo
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- CN210237634U CN210237634U CN201920941564.3U CN201920941564U CN210237634U CN 210237634 U CN210237634 U CN 210237634U CN 201920941564 U CN201920941564 U CN 201920941564U CN 210237634 U CN210237634 U CN 210237634U
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Abstract
An anaerobic medium preparation system for preparing an anaerobic microbial medium, comprising: the air pumping/inflating module is used for providing a vacuum source and an air source for the anaerobic culture medium preparation system; a control module; used for controlling the work of the anaerobic culture medium preparation system; the monitoring module is used for monitoring the air pressure of an air path in the anaerobic culture medium preparation system; the power supply module is used for supplying power to the anaerobic culture medium preparation system; the display input module is used for displaying working parameters and receiving control information for the anaerobic culture medium preparation system and transmitting the control information to the control module; the air pumping/inflating module, the monitoring module, the display input module and the power supply module are electrically connected with the control module. The utility model discloses can carry out the deoxidization of anaerobic microorganism culture medium and aerify automatically after the user sets up control information, avoid manual operation to produce the possibility of maloperation, promote seal and life, save test time, test efficiency improves by a wide margin.
Description
Technical Field
The utility model relates to an anaerobic culture medium preparation system belongs to anaerobic microorganism culture apparatus and makes technical field.
Background
The prior art method for preparing the anaerobic culture medium suitable for laboratories is generally a vacuum pumping method or a boiling oxygen-expelling method.
The vacuum pumping method comprises packaging the prepared anaerobic culture medium into containers, sealing, connecting vacuum pump with the containers via pipeline, pumping vacuum, filling oxygen-free gas, and repeating the above steps for several times. The boiling oxygen-removing method is that the prepared culture medium is put into a container, heated and boiled while oxygen-free gas is introduced, then cooled to room temperature under the condition of introducing oxygen, and then subpackaged and sterilized.
At present, the two preparation methods are manually carried out, the internal pressure of a container cannot be effectively adjusted in the preparation process, the preparation process is complicated, and potential safety hazards exist.
SUMMERY OF THE UTILITY MODEL
The utility model aims to solve the technical problem that not enough to prior art provides an anaerobism culture medium preparation system, can set up the deoxidization of control information back automatic anaerobism culture medium and aerify at the user, has avoided manual operation to produce the possibility of maloperation, has promoted seal and life, has saved test time, and test efficiency improves by a wide margin.
The utility model discloses the technical problem that solve is realized through following technical scheme:
the utility model provides an anaerobic culture medium preparation system for prepare anaerobic microorganism culture medium, anaerobic culture medium preparation system contains:
the air pumping/inflating module is used for providing a vacuum source and an air source for the anaerobic culture medium preparation system;
the control module is used for controlling the anaerobic culture medium preparation system to work;
the monitoring module is used for monitoring the air pressure of an air path in the anaerobic culture medium preparation system;
the power supply module is used for supplying power to the anaerobic culture medium preparation system; and
the display input module is used for displaying working parameters and receiving control information for the anaerobic culture medium preparation system and transmitting the control information to the control module;
the air pumping/inflating module, the monitoring module, the display input module, the power supply module and the control module are electrically connected.
Preferably, the air pumping/inflating module comprises a vacuum pump, an air source, an air channel and a sample bottle interface, the air channel is used for communicating the vacuum pump with the sample bottle interface and communicating the air source with the sample bottle interface, one end of the sample bottle interface is communicated with the air channel, and the other end of the sample bottle interface is communicated with the sample bottle.
The gas source comprises one or more of nitrogen, hydrogen and carbon dioxide according to different test conditions.
In order to improve the service life, the safety and the sealing performance of the anaerobic culture medium preparation system, the gas channel comprises a gas pipe, the gas pipe is made of polyurethane, and an interface of the gas pipe is a locking type gas interface.
In order to facilitate the on-off control of the air path, the air path further comprises a plurality of electromagnetic valves for controlling the on-off of the air path, and the electromagnetic valves are electrically connected with the control module.
Preferably, a first electromagnetic valve and a second electromagnetic valve are sequentially arranged between the vacuum pump and the sample bottle interface; and a third electromagnetic valve, a fourth electromagnetic valve and a second electromagnetic valve are sequentially arranged between the gas source and the sample bottle interface, wherein the number of the third electromagnetic valves is the same as the number of the gas types of the gas source.
In order to monitor the pressure of gas circuit, monitoring module contains the manometer, the manometer contains malleation table and negative pressure table, the negative pressure table sets up first solenoid valve with between the second solenoid valve, the malleation table sets up the gas outlet of air supply, and the third solenoid valve with between the fourth solenoid valve.
The user can control the speed of inflation to more accurate control inflation pressure, the fourth solenoid valve with still be equipped with the pressure governing valve between the second solenoid valve.
Preferably, the display input module comprises a touch screen.
Preferably, the anaerobic medium preparation system further comprises: one or more of a heat dissipation module, a time alarm module, a lighting module and a self-checking module.
To sum up, the utility model discloses anaerobic culture medium preparation system can set up automatic deoxidization and the inflation that carries out anaerobic microorganism culture medium after the control information at the user, has avoided manual operation to produce the possibility of maloperation, has promoted seal and life, has saved test time, and test efficiency improves by a wide margin.
The technical solution of the present invention will be described in detail with reference to the accompanying drawings and specific embodiments.
Drawings
FIG. 1 is a schematic structural diagram of an anaerobic culture medium preparation system of the present invention;
FIG. 2 is a schematic diagram of the gas circuit of the anaerobic culture medium preparation system of the present invention.
Detailed Description
Fig. 1 is a schematic structural diagram of the anaerobic culture medium preparation system 1 of the present invention, and fig. 2 is a schematic diagram of the gas circuit of the anaerobic culture medium preparation system 1 of the present invention. As shown in fig. 1 and 2, the present invention provides an anaerobic culture medium preparation system 1, wherein the anaerobic culture medium preparation system 1 comprises: the air extracting/inflating device comprises an air extracting/inflating module 100, a monitoring module 200, a display input module 300, a power supply module 500 and a control module 400, wherein the air extracting/inflating module 100, the monitoring module 200, the display input module 300, the power supply module 500 and the control module 400 are electrically connected.
The anaerobic culture medium preparation system is applied to automatic deoxidization and nitrogen N addition of anaerobic microorganism culture medium2Hydrogen gas H2And carbon dioxide CO2And other gases can be directly controlled to pump and inflate the circulation times and time through the display input module, so that the anaerobic preparation of the microorganism culture medium is realized. The anaerobic culture medium preparation system is widely applied and can be used for isolated culture of anaerobic microorganisms in soil, plant rhizosphere, human and animal oral cavities and intestinal tracts.
The air pumping/inflating module 100 is used for providing a vacuum source and an air source for the anaerobic culture medium preparation system, and comprises a vacuum pump 110, an air source 120, an air channel and a sample bottle interface 800, wherein the air channel comprises an air pipe and is used for communicating the vacuum pump 110 with the sample bottle interface 800 and communicating the air source 120 with the sample bottle interface 800, one end of the sample bottle interface 800 is communicated with the air channel, and the other end of the sample bottle interface 800 is used for communicating a sample bottle 900 (an anaerobic bottle). The present invention does not limit the structure of the sample bottle interface 800, as long as it can communicate with the sample bottle 900 and ensure the sealing performance between the two, for example, when the bottle cap of the sample bottle 900 is a rubber plug, the sample bottle interface 800 can be a needle tube, specifically, when the sample bottle 900 needs to be connected, the rubber plug can be penetrated through by the needle on the needle tube; when the needle is pulled out, the rubber stopper ensures the sealing of the sample bottle 900 under the action of its own elasticity. The utility model discloses do not restrict sample bottle interface 800 and the quantity of the gas channel who is connected with it, technical personnel in the field can design according to actual need, and in this embodiment, the quantity of sample bottle interface 800 is 8, and 8 sample bottles 900 of group can be connected simultaneously to anaerobism culture medium preparation system 1 promptly.
The utility model discloses do not restrict tracheal material, in order to prevent anaerobic culture medium preparation system 1 working process, the too big trachea that damages of gas pressure to improve tracheal life, the preferred material of trachea material is polyurethane, the highest withstand voltage is two spool of gluing for 145 PSI's three in this embodiment.
In order to ensure the firmness of the joint of the air pipe and other components, the interface of the air pipe is a locking type air interface instead of a traditional quick-plugging type air interface.
The vacuum pump 110 is communicated with the sample bottle 900 through an air pipe and the sample bottle interface 800, and is used for pumping out air (oxygen) in the sample bottle 900; the gas source 120 is preferably a gas cylinder which is communicated with the sample bottle 900 through a gas pipe and is used for filling the sample bottle 900 with gas in the gas cylinder. Preferably, the gas source 120 may comprise a plurality of gas cylinders, and different gas cylinders may contain different gases, for example, in this embodiment, the gas source 120 comprises 3 gas cylinders, each of which contains nitrogen N2Hydrogen gas H2And carbon dioxide CO2The present invention is not limited to this, and those skilled in the art can use other kinds of air sources according to actual needs.
In order to realize the break-make of gas circuit to aerify or bleed, the utility model discloses still contain a plurality of solenoid valves that are used for controlling the gas circuit break-make among the gas passage, solenoid valve and control module 400 electric connection, preferably, the solenoid valve is tribit five-way solenoid valve.
Specifically, a first electromagnetic valve 111 and a second electromagnetic valve 112 are sequentially arranged between the vacuum pump 110 and the sample bottle interface 800, when valves of the first electromagnetic valve 111 and the second electromagnetic valve 112 are both opened, a gas passage between the vacuum pump 110 and the sample bottle interface 800 is communicated, and the vacuum pump 110 can pump out gas in the sample bottle interface 800.
A third electromagnetic valve 121, a fourth electromagnetic valve 122 and a second electromagnetic valve 112 are sequentially arranged between the gas source 120 and the sample bottle interface 800, wherein the number of the third electromagnetic valves 121 is the same as the number of the gas species of the gas source, and a third electromagnetic valve 121 is arranged on a gas channel between each gas bottle and the sample bottle interface 800, so as to control the species of the gas source, for example, when the number is N, the type is controlled2The valve of the corresponding third electromagnetic valve 121 is opened, and H2And CO2When the corresponding valve of the third electromagnetic valve 121 is closed, the anaerobic medium preparing system 1The sample bottle 900 may be filled with N2。
The display input module 300 is configured to display working parameters and receive control information, and transmit the control information to the control module 400, and the control module 400 controls the anaerobic medium preparation system 1 to work according to the received control information. Specifically, the display input module 300 includes a touch screen or other types of display and control components, and a user can input various control information through the touch screen, where the control information includes, but is not limited to, one or more of working time (air pumping time and/or air inflation time), alarm time, cycle number, gas type, and the like. The working parameters include but are not limited to one or more of air path pressure, self-checking information and the like.
The present invention is not limited to this, for example, the display input module 300 may also include a display portion (e.g., a display screen) for displaying the operating parameters and an operation portion (e.g., a keyboard, a button or a knob) operated by the user for inputting the control information. In addition, the present invention is not limited to the anaerobic medium preparation system 1, all the control information is inputted by the display input module 300, in other words, other control components, such as a power switch, a lighting switch, a vacuum pump switch, etc. of the anaerobic medium preparation system 1 can be independently set on the anaerobic medium preparation system 1.
The monitoring module 200 is used for monitoring the air pressure of the air path in the anaerobic culture medium preparation system 1. The monitoring module 200 comprises a pressure gauge, the pressure gauge comprises a positive pressure gauge 210 and a negative pressure gauge 220, and the negative pressure gauge 220 is used for measuring the air pressure in the sample bottle 900 during the air extraction process; during the aeration process, the positive pressure gauge 210 is used to measure the air pressure in the air path of the anaerobic culture medium preparation system 1.
Specifically, the negative pressure gauge 220 is disposed on a gas passage between the sample bottle 900 and the vacuum pump 110, and more specifically, the negative pressure gauge 220 is disposed between the first solenoid valve 111 and the second solenoid valve 112, and in the pumping process function, the valves of the first solenoid valve 111 and the second solenoid valve 112 are both opened, and a user can obtain the pressure inside the sample bottle 900 through the negative pressure gauge 220, thereby guiding the user to operate.
In this embodiment, the number of the positive pressure gauge 210 is 2, one of the positive pressure gauges is disposed at the air outlet of the air source 120, so that a user can conveniently obtain the pressure of the air source 120, for example, when the air source 120 is an air cylinder, the user can determine whether to replace a new air cylinder or control the air outlet pressure of the air cylinder through an air cylinder pressure reducing valve according to the pressure in the air cylinder. And the other is disposed between the third solenoid valve 121 and the sample bottle interface 800, preferably, between the third solenoid valve 121 and the fourth solenoid valve 122, and the user can obtain the pressure inside the sample bottle 900 when the user inflates by observing the positive pressure gauge 210, thereby guiding the user to operate.
In order to facilitate the inflation of a user, a pressure regulating valve 123 is further disposed between the fourth electromagnetic valve 122 and the second electromagnetic valve 112, and the user can control the inflation speed by adjusting the pressure regulating valve 123, so as to more accurately control the inflation pressure.
The power module 500 is used for supplying power to the anaerobic culture medium preparation system 1, and preferably, the power module 500 further includes a touch control screen protection circuit, an ac/dc power conversion module, a power input safety circuit, and power output switch protection (applying ac and dc power input/output protection to ensure normal operation of the device). Specifically, a 1K resistor is connected in series inside a transistor output Y point in a touch control screen protection circuit; the alternating current-direct current power supply conversion module is a controller, the relay module supplies 24V direct current power, and the other electromagnetic valve supplies 220V alternating current power; in the power input safety loop: 16A insurance is added to the external main power supply, the standby power supply interface and the power supply interface of the vacuum pump 110; the power output switch protection comprises a second-stage protection switch of an external power supply and an external main power supply fuse. The above-mentioned setting is prior art, and the design can be selected by the person skilled in the art according to the actual need, and will not be described herein again.
In order to realize the confidentiality of the case of the anaerobic culture medium preparation system 1 and facilitate later-stage function expansion, the case body is preferably made of a white opaque acrylic plastic plate.
In order to realize circulation and dust removal in the case and ensure cleanness and heat dissipation in the case, a heat dissipation module such as a ventilation fan is arranged on the case.
In addition, the anaerobic medium preparation system 1 further comprises a time alarm module 600 for reminding a user of the working time. For example, when the aeration, aspiration or aeration/aspiration cycle of the anaerobic media preparation system 1 is completed, the time alarm module 600 beeps to indicate to the user that the sample can be removed or a further test can be performed.
In order to facilitate the user to use the anaerobic culture medium preparation system 1 in a dark place, the anaerobic culture medium preparation system 1 further comprises an illumination module 700 for providing illumination to the working area of the anaerobic culture medium preparation system 1.
In order to prevent the anaerobic medium preparation system from malfunctioning and affecting the test, the anaerobic medium preparation system further comprises a self-checking module 800 for checking whether the components in the anaerobic medium preparation system are working normally, and the checking result can be displayed on the display input module 300, or an indicator light can be additionally arranged to display the checking result. The self-checking items include, but are not limited to, whether the control module reaches a predetermined voltage active output, whether the PLC program is running, whether the touch screen is operating normally, whether there is an error in the control module, and the like.
It should be noted that, in order to ensure the air tightness of the anaerobic culture medium preparation system 1, shorten the vacuum-pumping time thereof, and the like, after the anaerobic culture medium preparation system 1 does not work or stops working, a plurality of valves of the electromagnetic valve are all in a closed state, and the utility model discloses do not use this as the limit.
The working process of the present invention will be described below by way of example.
First, after placing the sample medium into the sample bottle, the sample bottle is docked with the sample bottle interface. And turning on a power switch of the anaerobic culture medium preparation system, and starting after inputting control information such as working time, cycle times, gas types, alarm time and the like on a touch screen. For example, the pumping time is 30s, the inflation time is 30s, the number of cycles is 1, and the gas type is N2The alarm time is 60 s.
At the moment, under the control of the control module, the vacuum pump starts to work, the valves of the first electromagnetic valve and the second electromagnetic valve corresponding to the sample bottle are opened, and the valves of the third electromagnetic valve and the fourth electromagnetic valve are in a closed state, namely, the anaerobic culture medium preparation system is used for communicating an air path for pumping air and sealing an air path for inflating the anaerobic culture medium preparation system, and the anaerobic culture medium preparation system pumps air to the sample bottle. When the air pumping is finished, the valve of the first electromagnetic valve is closed and corresponds to N2The third electromagnetic valve, the fourth electromagnetic valve and the valve of the second electromagnetic valve corresponding to the sample bottle are opened, namely, the gas circuit for pumping air of the anaerobic culture medium preparation system is closed, and the gas circuit for inflating air is communicated, and the anaerobic culture medium preparation system injects N into the sample bottle2. Because the cycle number set in the test is 1, after the air exhaust and inflation are finished, the buzzer of the time alarm module sounds to prompt a user to take away the sample. The user may then perform the test again, or modify the control information and then perform the test. Preferably, a plurality of sets of control information can be preset in the control module so as to facilitate the user to select and use.
The utility model discloses well anaerobism culture medium preparation system can set up automatic deoxidization and the aerifing of anaerobic microorganism culture medium after the control information, has avoided manual operation to produce the possibility of maloperation, has promoted seal and life, has saved test time, and test efficiency improves by a wide margin.
Claims (10)
1. An anaerobic medium preparation system for preparing an anaerobic microbial medium, the anaerobic medium preparation system comprising:
the air pumping/inflating module is used for providing a vacuum source and an air source for the anaerobic culture medium preparation system;
the control module is used for controlling the anaerobic culture medium preparation system to work;
the monitoring module is used for monitoring the air pressure of an air path in the anaerobic culture medium preparation system;
the power supply module is used for supplying power to the anaerobic culture medium preparation system; and
the display input module is used for displaying working parameters and receiving control information for the anaerobic culture medium preparation system and transmitting the control information to the control module;
the air pumping/inflating module, the monitoring module, the display input module, the power supply module and the control module are electrically connected.
2. The anaerobic media preparation system of claim 1, wherein the gas evacuation/aeration module comprises a vacuum pump, a gas source, a gas channel for communicating the vacuum pump with the sample bottle interface, and a gas source with the sample bottle interface, the sample bottle interface communicating with the gas channel at one end and with the sample bottle at the other end.
3. The anaerobic medium production system of claim 2, wherein the gas source comprises one or more of nitrogen, hydrogen, and carbon dioxide.
4. The system for preparing anaerobic culture medium according to claim 2, wherein the gas channel comprises a gas tube made of polyurethane, and the interface of the gas tube is a locking gas interface.
5. The anaerobic culture medium preparation system of claim 4, wherein the gas channel further comprises a plurality of solenoid valves for controlling the on/off of the gas channel, and the solenoid valves are electrically connected with the control module.
6. The anaerobic medium preparation system of claim 5, wherein a first solenoid valve and a second solenoid valve are sequentially arranged between the vacuum pump and the sample bottle interface; and a third electromagnetic valve, a fourth electromagnetic valve and a second electromagnetic valve are sequentially arranged between the gas source and the sample bottle interface, wherein the number of the third electromagnetic valves is the same as the number of the gas types of the gas source.
7. The anaerobic media preparation system of claim 6, wherein the monitoring module comprises a pressure gauge comprising a positive pressure gauge and a negative pressure gauge, the negative pressure gauge disposed between the first solenoid valve and the second solenoid valve, the positive pressure gauge disposed at an outlet of the gas source, and between the third solenoid valve and the fourth solenoid valve.
8. The anaerobic medium preparation system of claim 7, wherein a pressure regulating valve is further disposed between the fourth solenoid valve and the second solenoid valve.
9. The anaerobic media preparation system of claim 1, wherein the display input module comprises a touch screen.
10. The anaerobic media preparation system of claim 1, further comprising: one or more of a heat dissipation module, a time alarm module, a lighting module and a self-checking module.
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| CN201920941564.3U CN210237634U (en) | 2019-06-21 | 2019-06-21 | Anaerobic culture medium preparation system |
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| CN201920941564.3U CN210237634U (en) | 2019-06-21 | 2019-06-21 | Anaerobic culture medium preparation system |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111905843A (en) * | 2020-08-11 | 2020-11-10 | 中国船舶工业集团公司第七0八研究所 | Marine modularization anaerobism laboratory system |
| CN112816678A (en) * | 2020-12-29 | 2021-05-18 | 广州市华代生物科技有限公司 | Method for detecting efficacy of oral product by using oral micro-ecological biomembrane model |
-
2019
- 2019-06-21 CN CN201920941564.3U patent/CN210237634U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111905843A (en) * | 2020-08-11 | 2020-11-10 | 中国船舶工业集团公司第七0八研究所 | Marine modularization anaerobism laboratory system |
| CN112816678A (en) * | 2020-12-29 | 2021-05-18 | 广州市华代生物科技有限公司 | Method for detecting efficacy of oral product by using oral micro-ecological biomembrane model |
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