CN220335153U - Anaerobic culture bottle preparation device - Google Patents
Anaerobic culture bottle preparation device Download PDFInfo
- Publication number
- CN220335153U CN220335153U CN202321346844.2U CN202321346844U CN220335153U CN 220335153 U CN220335153 U CN 220335153U CN 202321346844 U CN202321346844 U CN 202321346844U CN 220335153 U CN220335153 U CN 220335153U
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- Prior art keywords
- push rod
- electric push
- culture bottle
- valve
- preparation device
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- 238000002360 preparation method Methods 0.000 title claims abstract description 37
- 230000005540 biological transmission Effects 0.000 claims abstract description 20
- 239000007799 cork Substances 0.000 claims description 3
- 239000007789 gas Substances 0.000 description 8
- 230000001276 controlling effect Effects 0.000 description 3
- 239000001963 growth medium Substances 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 238000007789 sealing Methods 0.000 description 3
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- JCXJVPUVTGWSNB-UHFFFAOYSA-N nitrogen dioxide Inorganic materials O=[N]=O JCXJVPUVTGWSNB-UHFFFAOYSA-N 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a preparation device of an anaerobic culture bottle, which comprises an air source, a vacuum pump, a culture bottle, a first electromagnetic valve, a second electromagnetic valve and an automatic preparation device, wherein the air source is connected with the vacuum pump; the automatic preparation device comprises a first electric push rod, a second electric push rod, a valve needle and a transmission device; the end parts of the first electric push rod and the second electric push rod are fixedly provided with a valve needle; the air source is connected to a valve needle on the first electric push rod through a first electromagnetic valve; the vacuum pump is connected to a valve needle on the second electric push rod through a second electromagnetic valve; the transmission device is arranged below the first electric push rod and the second electric push rod, and a plurality of culture bottles are fixed on the transmission device. The electric push rod can automatically pierce the valve needle into the culture bottle for vacuumizing and inflating, and the automatic preparation of the anaerobic culture bottle is realized under the cooperation of the transmission device.
Description
Technical Field
The utility model relates to a biological culture medium, in particular to a preparation device of an anaerobic culture bottle.
Background
The vacuum pumping method is to split-load the prepared anaerobic culture medium into containers for sealing, then connect a vacuum pump with the containers through a pipeline for vacuumizing, then fill oxygen-free gas, and repeat the process for a plurality of times. The boiling and oxygen-expelling method is to put the prepared culture medium into a container, heat and boil, introduce oxygen-free gas at the same time, cool to room temperature under the condition of aeration, and then split charging and sterilization are carried out.
At present, the two preparation methods need to participate in all or part of steps manually, the internal pressure of a container cannot be effectively regulated in the preparation process, the preparation process is complicated, and when a large number of culture bottles are required for experiments, the time and labor cost for preparing the culture bottles are relatively high.
Disclosure of Invention
In view of the above-mentioned shortcomings of the existing anaerobic culture bottle preparation methods, the utility model provides an anaerobic culture bottle preparation device, which can achieve the effect of automatic batch preparation of anaerobic culture bottles.
In order to achieve the above purpose, the embodiment of the present utility model adopts the following technical scheme:
an anaerobic culture bottle preparation device comprises an air source, a vacuum pump, a culture bottle, a first electromagnetic valve, a second electromagnetic valve and an automatic preparation device; the automatic preparation device comprises a first electric push rod, a second electric push rod, a valve needle and a transmission device; the end parts of the first electric push rod and the second electric push rod are fixedly provided with a valve needle; the air source is connected to a valve needle on the first electric push rod through a first electromagnetic valve; the vacuum pump is connected to a valve needle on the second electric push rod through a second electromagnetic valve; the transmission device is arranged below the first electric push rod and the second electric push rod, and a plurality of culture bottles are fixed on the transmission device.
A flow control valve is arranged between the air source and the first electromagnetic valve and used for controlling the flow of air released by the air source.
The device also comprises a positive pressure meter; the number of the positive pressure tables is two; the air outlet end is arranged at the air source; the other is disposed between the first solenoid valve and the flow control valve.
The negative pressure meter is also included; the negative pressure meter is arranged between the second electromagnetic valve and the valve needle on the second electric push rod, can be used for detecting the air pressure inside the culture bottle and is used for helping to judge whether the anaerobic culture bottle is successfully prepared.
The bottleneck of blake bottle is rubber cork, and the needle of being convenient for pierces, and the needle is pulled out inside still can keep sealing state.
The implementation of the utility model has the advantages that:
the needle is arranged on the electric push rod, the culture bottle is placed on the transmission device, the needle is automatically stretched into the culture bottle through the electric push rod, the automatic vacuumizing and the inflating are carried out under the cooperation of the first electromagnetic valve and the second electromagnetic valve, the preparation of the current culture bottle is completed after repeated times, the electric push rod is retracted, the needle is retracted, the transmission device moves the next culture bottle to the current position to continue the preparation of the anaerobic culture bottle, and therefore batch automatic preparation of the anaerobic culture bottle is achieved.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present utility model, the drawings that are needed in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present utility model, and other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
Fig. 1 is a schematic structural diagram of an anaerobic culture bottle preparation device according to the present utility model.
Legend description: 1. a gas source; 11. a first electromagnetic valve; 12. a flow control valve; 2. a vacuum pump; 21. a second electromagnetic valve; 3. a culture bottle; 41. a first electric push rod; 42. a second electric push rod; 43. a valve needle; 44. a transmission device; 5. a positive pressure gauge; 6. negative pressure meter.
Detailed Description
The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
As shown in fig. 1, the anaerobic culture bottle 3 preparation device comprises an air source 1, a vacuum pump 2, a culture bottle 3, a first electromagnetic valve 11, a second electromagnetic valve 21 and an automatic preparation device; the automatic preparation device comprises a first electric push rod 41, a second electric push rod 42, a valve needle 43 and a transmission device 44; the valve needle 43 is fixed at the end parts of the first electric push rod 41 and the second electric push rod 42; the air source 1 is connected to a valve needle 43 on the first electric push rod 41 through a first electromagnetic valve 11; the vacuum pump 2 is connected to a valve needle 43 on a second electric push rod 42 through a second electromagnetic valve 21; the transmission device 44 is arranged below the first electric push rod 41 and the second electric push rod 42, and a plurality of culture bottles 3 are fixed on the transmission device;
the valve needle 43 is arranged on the electric push rod, the culture bottle 3 is arranged on the transmission device 44, the valve needle 43 is automatically stretched into the culture bottle 3 through the electric push rod, automatic vacuumizing and inflating are carried out under the cooperation of the first electromagnetic valve 11 and the second electromagnetic valve 21, the preparation of the current culture bottle 3 is completed after repeated times, the electric push rod is retracted, the valve needle 43 is retracted, the transmission device 44 moves the next culture bottle 3 to the current position to continuously carry out the preparation of the anaerobic culture bottle 3, and therefore batch automatic preparation of the anaerobic culture bottle 3 is realized.
A flow control valve 12 is arranged between the air source 1 and the first electromagnetic valve 11 and is used for controlling the flow of the air released by the air source 1; in practical applications, the gas source 1 typically comprises a cylinder of hydrogen, nitrogen and carbon dioxide; the outlet gas path of each gas cylinder is respectively provided with a flow control valve 12 for precisely controlling the proportion of each gas component in the inflation gas.
Positive pressure table 5 is also included; the number of the positive pressure tables 5 is two; the air outlet end is arranged at the air source 1; the other is arranged between the first electromagnetic valve 11 and the flow control valve 12; the positive pressure gauge 5 may be used to detect the air pressure of the air supply 1 to determine if the air supply 1 needs to be replaced.
The negative pressure meter 6 is also included; the negative pressure meter 6 is disposed between the second electromagnetic valve 21 and the valve needle 43 on the second electric push rod 42, and can be used for detecting the air pressure inside the culture flask 3, and for helping to determine whether the anaerobic culture flask 3 is successfully prepared.
The bottle mouth of the culture bottle 3 is a rubber cork, so that the valve needle 43 is convenient to penetrate, and the inside of the culture bottle can still keep a sealing state within a certain time after the valve needle 43 is pulled out.
The implementation of the utility model has the advantages that:
through installing the needle on electric putter, the blake bottle then places on transmission, pierces the needle inside the blake bottle through electric putter is automatic flexible, carries out automatic evacuation, aerifys under the cooperation of first solenoid valve, second solenoid valve, accomplishes the preparation of current blake bottle after repeated many times, and electric putter withdraws, withdraws the needle, and transmission removes next blake bottle to current position and continues the preparation of anaerobic blake bottle to realize anaerobic blake bottle's batch automatic preparation.
The foregoing is merely illustrative of the present utility model, and the present utility model is not limited thereto, and any changes or substitutions easily contemplated by those skilled in the art within the technical scope of the present utility model should be included in the scope of the present utility model. Therefore, the protection scope of the present utility model shall be subject to the protection scope of the claims.
Claims (6)
1. The preparation device of the anaerobic culture bottle (3) comprises an air source (1), a vacuum pump (2) and the culture bottle (3), and is characterized by further comprising a first electromagnetic valve (11), a second electromagnetic valve (21) and an automatic preparation device; the automatic preparation device comprises a first electric push rod (41), a second electric push rod (42), a valve needle (43) and a transmission device (44); the valve needle (43) is fixed at the end parts of the first electric push rod (41) and the second electric push rod (42); the air source (1) is connected to a valve needle (43) on the first electric push rod (41) through a first electromagnetic valve (11); the vacuum pump (2) is connected to a valve needle (43) on a second electric push rod (42) through a second electromagnetic valve (21); the transmission device (44) is arranged below the first electric push rod (41) and the second electric push rod (42), and a plurality of culture bottles (3) are fixed on the transmission device.
2. Anaerobic culture flask (3) preparation device according to claim 1, characterized in that a flow control valve (12) is arranged between the gas source (1) and the first solenoid valve (11).
3. The anaerobic culture bottle (3) preparation device according to claim 1, further comprising a positive pressure meter (5).
4. An anaerobic culture flask (3) preparation device according to claim 3, wherein the number of positive pressure gauges (5) is two; the air outlet end is arranged at the air source (1); the other is arranged between the first electromagnetic valve (11) and the flow control valve (12).
5. The anaerobic culture bottle (3) preparation device according to claim 1, further comprising a negative pressure meter (6); the negative pressure gauge (6) is arranged between the second electromagnetic valve (21) and a valve needle (43) on the second electric push rod (42).
6. The anaerobic culture bottle (3) preparation device according to claim 1, wherein the bottle mouth of the culture bottle (3) is a rubber cork.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321346844.2U CN220335153U (en) | 2023-05-30 | 2023-05-30 | Anaerobic culture bottle preparation device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321346844.2U CN220335153U (en) | 2023-05-30 | 2023-05-30 | Anaerobic culture bottle preparation device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220335153U true CN220335153U (en) | 2024-01-12 |
Family
ID=89458514
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321346844.2U Active CN220335153U (en) | 2023-05-30 | 2023-05-30 | Anaerobic culture bottle preparation device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220335153U (en) |
-
2023
- 2023-05-30 CN CN202321346844.2U patent/CN220335153U/en active Active
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