CN202214355U - Intelligent anaerobic environment generation experimental system - Google Patents

Intelligent anaerobic environment generation experimental system Download PDF

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Publication number
CN202214355U
CN202214355U CN 201120347501 CN201120347501U CN202214355U CN 202214355 U CN202214355 U CN 202214355U CN 201120347501 CN201120347501 CN 201120347501 CN 201120347501 U CN201120347501 U CN 201120347501U CN 202214355 U CN202214355 U CN 202214355U
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pipeline
vacuum
cylinder
anaerobic environment
rare gas
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刘增辉
苏东海
朱运利
辛秀兰
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Beijing Polytechnic
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Beijing Polytechnic
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/40Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M45/00Means for pre-treatment of biological substances
    • C12M45/02Means for pre-treatment of biological substances by mechanical forces; Stirring; Trituration; Comminuting
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M45/00Means for pre-treatment of biological substances
    • C12M45/20Heating; Cooling

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Abstract

An intelligent anaerobic environment generation experimental system belongs to the field of biomedicine, and comprises a pipeline, a vacuum pipeline and main pipelines, wherein the pipeline is filled with inert gas, and each main pipeline is connected with an experimental container. Pressure sensors are arranged on the pipeline filled with inert gas and the vacuum pipeline, and electromagnetic valves for separating are arranged between the pipeline filled with the inert gas and each main pipeline, between the vacuum pipeline and each main pipeline, between an inert gas cylinder and the pipeline, and between a vacuum pump and the vacuum pipeline. Each main pipeline is connected with the upper end of a cylinder rod through a connector, the lower end of each cylinder rod is connected with a syringe filter and a syringe needle, two magnetic inductive proximity switches for judging puncturing positions are mounted at two ends of each driving cylinder, each experimental container with a rubber bottle plug is placed below the corresponding syringe needle, and the magnetic inductive proximity switches, the electromagnetic valves and the pressure sensors are connected onto a PLC (programmable logic controller) control system which is connected with a touch screen. The intelligent anaerobic environment generation experimental system is capable of conveniently realizing anaerobic environment generation directly in small-capacity containers, so that anaerobic cultivation experiments can be carried out.

Description

Intelligent anaerobic environment generation experimental system
Technical field:
The utility model is a kind of intelligent full-automatic experimental installation that is applied to biomedicine field, and it can be conveniently implemented in the purpose that anaerobic environment directly takes place in the small volume containers, to carry out the anaerobism culture experiment.It has overcome shortcomings such as the operation inconvenience that brings when traditional anaerobism incubator experimentizes, anaerobic environment formation time length, energy consumption height under " overall situation "; Also realized the function that multi-work-station carries out work simultaneously simultaneously at an easy rate; Can realize the purpose of experiment in batches at an easy rate, and have very high experimental repeatability.
Background technology:
The anaerobism culture experiment is the modal a kind of experiment in biological study field; Common laboratory facilities are in an airtight incubator, to form anaerobic environment; The operator puts in both hands to have gloves in the incubator and operates; Receive the restriction of operational condition, the accessibility of operation and the handiness of operation all receive very big restriction, and this just requires the operator must grasp certain operating skill just can accomplish to accomplish safely experiment.In addition, traditional anaerobism incubator is in whole incubator, to form the method for anaerobic environment owing to what adopt, so have shortcomings such as the anaerobic environment formation time is long, efficient is low, operation inconvenience inevitably.
The utility model content:
In order to overcome existing many shortcomings when utilizing the anaerobism incubator to carry out the anaerobism culture experiment; The anaerobic environment generation experimental system that the utility model provides adopts complete intelligentized mode, and the equipment that makes has simple to operate; Experimental repeatability is good; The anaerobic environment formation time is short, and energy consumption is low, configurable different advantages such as anaerobic environment.In addition, the design of multi-work-station and high-level efficiency make this equipment be suitable for carrying out the batch experiment.
The utility model provides a kind of intelligent anaerobic environment generation experimental system; Intelligent anaerobic environment generation experimental system; It is characterized in that: it comprises the pipeline that is used for the filling rare gas element that is connected with the rare gas element steel cylinder; Be connected with the vacuum pipe that is used for extracting vacuum of vacuum pump, be used for the main pipe line that is connected with experiment container; The said pipeline that is used for the filling rare gas element all is equipped with pressure transmitter with the vacuum pipe that is used for extracting vacuum, and the pipeline that is used for the filling rare gas element communicates with the vacuum pipe and the main pipe line that are used for extracting vacuum;
Be used between the pipeline and main pipe line of filling rare gas element, between vacuum pipe and the main pipe line, rare gas element steel cylinder and be used between the pipeline of filling rare gas element, all have SV to isolate between vacuum pump and the vacuum pipe.
Main pipe line is connected with the upper end of cylinder rod through connecting joint, and the lower end of cylinder rod is connecting the pin type strainer, and the lower end of pin type strainer is connecting entry needle.
Drive the cylinder two ends and two magnetic inductives that carry out the puncture position judgement are housed near switch.
The experiment container that has bottle closure of rubber is placed on the below of entry needle.
Above-mentioned magnetic inductive all is connected on the system near switch, SV and pressure transmitter, and system connects indicating system.
Further, the lower end of cylinder rod is also connecting the anti-drop device of being made up of anti-drop device seat and anti-drop device lid, and entry needle connects with the pin type strainer earlier, is installed in then on the anti-drop device seat, and the pin type strainer is in anti-drop device.
The technical scheme that the utility model adopted is: adopt independently anaerobism subenvironment experiment model; Promptly when experiment; Select to load experimental solutions with the vial (generally selecting volume about 100ml) of rubber stopper seal; Then stainless steel entry needle prior and that experimental channel connects is thrust in the vial from the rubber plug top, accomplish experimental installation and the quick of experiment container and be connected.Utilize this experimental channel (below be called main pipe line) realize the vacuumizing of vial experiment container, inject need filling gas (like N 2, CO 2).There are two laterals the rear end of main pipe line, and one is connected with vacuum pump, and another source of the gas with inflation is connected, between main pipe line and the small transfer line through the solenoid control connecting and disconnecting.The transmitter that detects vacuum tightness and positive pressure is installed in main pipe line; Be used to realize the pressure process that vacuumizes with filling gas is detected; SV all is connected with system (like PLC, various microcomputer control system) with transmitter, through the user program that stores in the system whole experimental system is realized automated procedure control.The utility model from entry needle be punctured to anaerobic environment forming process, automatically realize by experimental installation until the entire operation control process of extracting of entry needle.
Because what adopt is thicker bottle closure of rubber sealing and thinner entry needle, therefore, after anaerobic environment produces completion and extracts entry needle; Can keep sealed state preferably; But to be on the safe side, after the anaerobic environment manufacturing is accomplished, if can be better with wax oil needle seal mouth effect.
Description of drawings:
Anaerobic environment process synoptic diagram takes place in Fig. 1 entry needle puncture method fast
The intelligent anaerobic environment generation of Fig. 2 experimental system ultimate principle
Fixing and the detection principle schematic of Fig. 3 experiment container
Embodiment:
Intelligent anaerobic environment generation experimental system is characterized in that it comprises the pipeline 1 that is used for the filling rare gas element, is used for the vacuum pipe 2 of extracting vacuum, is used for the main pipe line 3 that is connected with experiment container; The said pipeline 1 that is used for the filling rare gas element all is equipped with pressure transmitter with the vacuum pipe 2 that is used for extracting vacuum.
Be used between the pipeline 1 and main pipe line 3 of filling rare gas element, between vacuum pipe 2 and the main pipe line 3, between rare gas element steel cylinder and the pipeline 1, all have SV to isolate between vacuum pump and the vacuum pipe 2.
Main pipe line 3 is connected through the upper end of connecting joint 4 with cylinder rod 5, and the lower end of cylinder rod 5 is connecting pin type strainer 10, and the lower end of pin type strainer 10 is connecting entry needle 12.
Drive cylinder 7 two ends and two magnetic inductives that carry out the puncture position judgement are housed near switch.
The experiment container that has bottle closure of rubber is placed on the below of entry needle 12.
Above-mentioned magnetic inductive all is connected on the PLC system near switch, SV and pressure transmitter, and the PLC system connects indicating system.
Further, the lower end of cylinder rod 5 is also connecting the anti-drop device of being made up of anti-drop device seat 9 and anti-drop device lid 11, and entry needle 12 connects with pin type strainer 10 earlier, is installed in then on the anti-drop device seat 9, and pin type strainer 10 is in anti-drop device.
1. the preparation of experiment container
The experiment container that native system requires to use is necessary for employing bottle closure of rubber sealed glass bottle, and generally below 500ML, rubber plug thickness is generally 10-15MM to volume, to guarantee to have better seal property.
2. experiment container stationary installation
When experiment, experiment container is to need fixed, and native system adopts the cylinder driving-type anchor clamps that experiment container is fixed on preposition, reaches to make the constant purpose in experiment container holding position in whole experiment.Fixing and the detection principle schematic of experiment container is seen Fig. 3.In Fig. 3, clamp cylinder 16 (below be called clamping cylinder) and be used to provide clamping force, what adopt among the design is double-acting cylinder.Front end at its cylinder rod 19 is installed with active chuck 20, the yielding rubber that on the front end face of active chuck 20, has been adhesively fixed, and when clamping, playing shock absorption increases frictional force simultaneously.Fixed stop 21 transverse section are V-type, and experiment container is played fixing and positioning action.17-transmitter S3 and 18-transmitter S4 have been installed respectively in the rear and front end of clamping cylinder 16, have been respectively applied for the movement position that detects cylinder rod, promptly detecting is releasing orientation or clamped condition, and what two transmitters all adopted is that magnetic inductive is near switch.S3 is used to judge whether to unclamp, and S4 is used to judge whether to clamp.
3. experiment container and experimental system is connected
Experiment container need form airtight the connection with the pipeline of experimental system during experiment, and the mode of connection that the utility model adopts is the entry needle puncture method.Specific practice is: adopt that a hollow is two to be gone out rod-type double-acting cylinder 7 (below be called the puncture cylinder) and as drive unit, drive the puncture action of entry needle.The cylinder rod 5 of this cylinder is an open core type; Can the double as gas circuit; Main pipe line 3 is connected through the upper end of connecting joint 4 with cylinder rod 5, and the lower end of cylinder rod 5 is connecting anti-drop device (being made up of anti-drop device seat 9 and anti-drop device lid 11), is used to prevent that entry needle from coming off when extracting; Entry needle 12 connects with pin type strainer 10 earlier, is installed in then on the anti-drop device seat 9, screws on anti-drop device lid 11 after installing.The relation of installation is seen Fig. 1.
When needs are connected experiment container with experimental system, just drive the bottle closure of rubber that entry needle thrusts experiment container fast through driving cylinder 7, realize being communicated with fast through entry needle and experiment container.Piercing process is seen Fig. 1.
The movement position that the drives cylinder state that just punctures can detect judgement near switch sensor 6 and transmitter 8 through being installed in the magnetic inductive that drives the cylinder two ends.Transmitter 6 is used to judge whether entry needle is extracted, and transmitter 8 is used to judge whether entry needle thrusts.
4. experimental channel system
The experimental channel of the utility model comprises: be used for filling N 2Gas (or filling CO 2Gas) rare gas element pipeline 1 is used for the vacuum pipe 2 of extracting vacuum, is used for the main pipe line 3 that is connected with experiment container.Between rare gas element pipeline 1 and the main pipe line 3, between vacuum pipe 2 and the main pipe line 3, between rare gas element steel cylinder and the pipeline 1, all have SV (like V1, V2) to isolate between vacuum pump and the vacuum pipe 2, open automatically through system when needed.The tubing system structure is seen Fig. 2.
5. process monitoring device
In when experiment, whole experiment all has corresponding transmitter to monitor, and has in the main monitoring: experiment container whether clamped fixing, whether carried out puncture, vacuumized that the vacuum tightness that whether has reached preset requires, whether filling gas is accomplished etc.
Particular case is following:
● whether 6-transmitter S1: magnetic inductive is installed on the upper end position that drives cylinder 7 near switch, be used to judge whether do not extract at puncture position or puncture completion entry needle really.See Fig. 1.
● 8-transmitter S2: magnetic inductive is installed on the lower end position that drives cylinder 7 near switch, is used to judge whether perhaps whether be punctured to the position at puncture position.See Fig. 1.
● 17-transmitter S3: magnetic inductive is installed on the back-end location of clamping cylinder 16 near switch, is used to judge whether clamping cylinder unclamps really.See Fig. 3.
● 18-transmitter S4: magnetic inductive is installed on the front position of clamping cylinder 16 near switch, is used to judge whether clamping cylinder has clamped experiment container really.See Fig. 3.The installation site of transmitter can be adjusted according to the diameter of experiment container.
● 22-pressure transmitter SN: be used for monitoring the inflation pressure of nitrogen pipeline 1, judge whether to have reached the inflation pressure requirement.
● 23-pressure transmitter SV: be used for monitoring the vacuum pressure of vacuum pipe 2, judge to vacuumize whether reached the vacuum tightness requirement.
In the sensor, transmitter 6 and transmitter 8, transmitter 17 and transmitter 18 are merely the service condition of a station, and other stations are all identical.
6. process control
The utility model equipment adopts programmable logic controller (PLC) control techniques and touch-screen control techniques to realize the process control to total system, makes system realize full automatic control.Touch-screen is the interface of man-machine interaction, is mainly used in the purpose of sending operational order with the demonstration of demonstration, experimentation, alarm display, to equipment that presets of experiment parameter; PLC is used to realize the intelligent process control of total system.
(1)PLC
The utility model equipment adopts PLC that total system is carried out robotization control; Native system needs digital quantity to import 23 points altogether; Digital quantity is exported 32 points; According to the requirement that IO are counted, can select siemens S7-200 (like the model of CPU more than 222), also can select the PLC of siemens S7-300 series.If do not consider extended function, select S7-200CPU both economical.In the utility model equipment, adopted the CPU 314 of S7-300 series, the main configuring condition of its hardware is seen table 1.
The main configuring condition of table 1PLC hardware
Sequence number Module Quantity
1 Power module PS 307 5A 1
2 The CPU module, CPU 314 1
3 Signaling module, SM 321 1
4 Signaling module, SM 322 1
The I/O address distribution condition of PLC is seen table 2.
The I/O address distribution condition of table 2PLC
Sequence number The address Symbol Equipment Effect
1 I0.0 1S1 Magneticinduction is near switch Judge the upper end position of puncture cylinder 1
2 I0.1 1S2 Magneticinduction is near switch Judge the lower end position of puncture cylinder 1
3 I0.2 1S3 Magneticinduction is near switch Judge the release position of clamping cylinder 1
4 I0.3 1S4 Magneticinduction is near switch Judge the clamped position of clamping cylinder 1
5 I0.4 2S1 Magneticinduction is near switch Judge the upper end position of puncture cylinder 2
6 I0.5 2S2 Magneticinduction is near switch Judge the lower end position of puncture cylinder 2
7 I0.6 2S3 Magneticinduction is near switch Judge the release position of clamping cylinder 2
8 I0.7 2S4 Magneticinduction is near switch Judge the clamped position of clamping cylinder 2
9 I1.0 3S1 Magneticinduction is near switch Judge the upper end position of puncture cylinder 3
10 I1.1 3S2 Magneticinduction is near switch Judge the lower end position of puncture cylinder 3
11 I1.2 3S3 Magneticinduction is near switch Judge the release position of clamping cylinder 3
12 I1.3 3S4 Magneticinduction is near switch Judge the clamped position of clamping cylinder 3
13 I1.4 4S1 Magneticinduction is near switch Judge the upper end position of puncture cylinder 4
14 I1.5 4S2 Magneticinduction is near switch Judge the lower end position of puncture cylinder 4
15 I1.6 4S3 Magneticinduction is near switch Judge the release position of clamping cylinder 4
16 I1.7 4S4 Magneticinduction is near switch Judge the clamped position of clamping cylinder 4
17 I2.0 5S1 Magneticinduction is near switch Judge the upper end position of puncture cylinder 5
18 I2.1 5S2 Magneticinduction is near switch Judge the lower end position of puncture cylinder 5
19 I2.2 5S3 Magneticinduction is near switch Judge the release position of clamping cylinder 5
20 I2.3 5S4 Magneticinduction is near switch Judge the clamped position of clamping cylinder 5
21 I2.4 SN Pressure transmitter Judge the inflated with nitrogen inflation pressure
22 I2.5 SV Pressure transmitter Judge evacuated pressure
23 I2.6 N-A Emergency stop switch Urgent stop control signal
24 Q4.4 KM1 Relay1 coil The power supply of control vacuum pump
25 Q4.1 KM2 Relay1 coil The red colored lamp of three layers of PL of control
26 Q4.2 KM3 Relay1 coil The yellow light of three layers of PL of control
27 Q4.3 KM4 Relay1 coil The green light of three layers of PL of control
28 Q4.4 KM5 Relay1 coil The control hummer
29 Q4.5 1V Electromagnetic valve coil The vacuum tunnel of control station 1
30 Q4.6 1N Electromagnetic valve coil The channel of nitrogen of control station 1
31 Q4.7 1Y1 Electromagnetic valve coil Control puncture cylinder 1 descends
32 Q5.0 1Y2 Electromagnetic valve coil Control puncture cylinder 1 rises
33 Q5.5 1Y3 Electromagnetic valve coil Control clamping cylinder 1
34 Q5.2 2V Electromagnetic valve coil The vacuum tunnel of control station 2
35 Q5.3 2N Electromagnetic valve coil The channel of nitrogen of control station 2
36 Q5.4 2Y1 Electromagnetic valve coil Control puncture cylinder 2 descends
37 Q5.5 2Y2 Electromagnetic valve coil Control puncture cylinder 2 rises
38 Q5.6 2Y3 Electromagnetic valve coil Control clamping cylinder 2
39 Q5.7 3V Electromagnetic valve coil The vacuum tunnel of control station 3
40 Q6.0 3N Electromagnetic valve coil The channel of nitrogen of control station 3
41 Q6.1 3Y1 Electromagnetic valve coil Control puncture cylinder 3 descends
42 Q6.2 3Y2 Electromagnetic valve coil Control puncture cylinder 3 rises
43 Q6.3 3Y3 Electromagnetic valve coil Control clamping cylinder 3
44 Q6.4 4V Electromagnetic valve coil The vacuum tunnel of control station 4
45 Q6.5 4N Electromagnetic valve coil The channel of nitrogen of control station 4
46 Q6.6 4Y1 Electromagnetic valve coil Control puncture cylinder 4 descends
47 Q6.7 4Y2 Electromagnetic valve coil Control puncture cylinder 4 rises
48 Q7.0 4Y3 Electromagnetic valve coil Control clamping cylinder 4
49 Q7.1 5V Electromagnetic valve coil The vacuum tunnel of control station 5
50 Q7.2 5N Electromagnetic valve coil The channel of nitrogen of control station 5
51 Q7.3 5Y1 Electromagnetic valve coil Control puncture cylinder 5 descends
52 Q7.4 5Y2 Electromagnetic valve coil Control puncture cylinder 5 rises
53 Q7.5 5Y3 Electromagnetic valve coil Control clamping cylinder 5
54 Q7.6 N Electromagnetic valve coil The break-make of control rare gas element pipeline
55 Q7.7 V Electromagnetic valve coil The break-make of control vacuum pipe
(2) touch-screen
Touch-screen is the equipment that carries out man-machine interaction, and in the design, operator send operational order through touch-screen to system, and realizes presetting and show, realize demonstration and alarm condition demonstration to experimentation experiment parameter through touch-screen.
In the utility model design, selection be siemens touch-screen TP 177B, also can select the touch-screen of other compatible with it brands.
The operation that on touch-screen, can carry out has:
1. operating mode is selected: the utility model is provided with two kinds of operator schemes, and promptly automatic mode and manual mode are selected through one " switch ".
Under automatic mode, operate " startup " switch, can realize the fully automatic operation of equipment, promptly equipment is automatically accomplished from being punctured to according to the parameter that is provided with in advance and is produced whole working process of extracting entry needle after anaerobic environment is accomplished.In automatic operational process, if operated " stopping " switch, then equipment can be extracted entry needle after having accomplished current working cycle (beginning to end up being to inflated with nitrogen a working cycle from vacuumizing) and quit work from experiment container.Under automatic mode, operation " resetting " switch is invalid.
Under " manually " pattern, operation " startups " switch can equipment be automatically accomplished from being punctured to according to the process of accomplishing a working cycle and is produced whole working process of extracting entry needle after the anaerobic environment completion.Under this pattern, operation " stopping " switch is invalid, if operation " resetting " switch, then equipment can automatically return to original state.
2. operation automatically: under " automatically " pattern, operation " startups " switch can equipment be automatically accomplished from being punctured to according to the parameter of setting in advance and is produced whole working process of extracting entry needle after the anaerobic environment completion.
3. out of service: under " automatically " pattern, operation " stopping " switch can make equipment after having accomplished current working cycle, entry needle extracted from experiment container and quit work.Be that a kind of user of giving ends the right of operation automatically.
4. manually single loop operation: under " manually " pattern, operation " startup " switch can make equipment automatically accomplish from being punctured to according to the process of accomplishing a working cycle and produce whole working process of extracting entry needle after anaerobic environment is accomplished.
5. reset operation: under " manually " pattern, operation " resetting " switch can make equipment automatically restore to original state.
6. parameter setting: in the utility model, need parameter, have only " cycle index " through the touch-screen setting.The size of cycle index influences the epoxy amount of anaerobic environment, and highly purified if desired anaerobic environment then need be provided with bigger cycle values.The scope that is provided with of cycle values: 1-10.
7. station is selected: the utility model has adopted the structure design of multi-work-station, promptly can be that a plurality of experiment containers are made anaerobic environment simultaneously.Need according to general experiment, in design actual design 5 stations, and, be provided with obsolete station carried out the function that selectivity is closed in order to make more convenient application.According to actual needs, can the station quantity of real work be selected between 1-5 through operation, minimum 1, maximum 5.
(3) anxious stopping operated
When emergency situation has appearred in equipment, need make equipment out of service immediately through certain operation, Here it is, and anxious stopping operated.The mode that directly is connected with the input port of PLC through the scram button switch has been adopted in the utility model design, so that PLC can full out receive the anxious order that stops.
7. other system equipment
In the utility model design, also need some other standard equipment; Mainly contain air (be used to provide cylinder operation required pressurized air); Vacuum pump (it directly is connected with vacuum pipe 2, is the equipment that is used for extracting vacuum), nitrogen (or other bodies of inflating) storage cylinder fitted.
Be this unit type of testing novel employing below:
Air: Lip river, Mount Tai, Shanghai industry and trade ltd produces, DA-500/4C
Vacuum pump: German KNF (assembling), KNF N816.3KPE
Nitrogen steel cylinder: WGA140-6-15.

Claims (2)

1. intelligent anaerobic environment generation experimental system is characterized in that: it comprises the pipeline that is used for the filling rare gas element that is connected with the rare gas element steel cylinder, is connected with the vacuum pipe that is used for extracting vacuum of vacuum pump, is used for the main pipe line that is connected with experiment container; The said pipeline that is used for the filling rare gas element all is equipped with pressure transmitter with the vacuum pipe that is used for extracting vacuum, and the pipeline that is used for the filling rare gas element communicates with the vacuum pipe and the main pipe line that are used for extracting vacuum;
Be used between the pipeline and main pipe line of filling rare gas element, between vacuum pipe and the main pipe line, rare gas element steel cylinder and be used between the pipeline of filling rare gas element, all have SV to isolate between vacuum pump and the vacuum pipe;
Main pipe line is connected with the cylinder rod upper end that drives cylinder through connecting joint, and the lower end of cylinder rod is connecting the pin type strainer, and the lower end of pin type strainer is connecting entry needle;
Drive the cylinder two ends and two magnetic inductives that carry out the puncture position judgement are housed near switch;
The experiment container that has bottle closure of rubber is placed on the below of entry needle;
Above-mentioned magnetic inductive all is connected on the system near switch, SV and pressure transmitter, and system connects indicating system.
2. according to the said intelligent anaerobic environment generation experimental system of claim 1; It is characterized in that: the lower end of cylinder rod is also connecting the anti-drop device of being made up of anti-drop device seat and anti-drop device lid; Entry needle connects with the pin type strainer earlier; Be installed in then on the anti-drop device seat, the pin type strainer is in anti-drop device.
CN 201120347501 2011-09-16 2011-09-16 Intelligent anaerobic environment generation experimental system Expired - Fee Related CN202214355U (en)

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Application Number Priority Date Filing Date Title
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Application Number Priority Date Filing Date Title
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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102015007049A1 (en) * 2015-05-29 2016-12-01 Lukas Neuhaus Method with an electronic sample fluid receiver for producing and providing fluid samples filled in environmentally sealed containers for analysis and / or limit monitoring
DE102015007050A1 (en) * 2015-05-29 2016-12-01 Lukas Neuhaus Method with an electronic switching valve-based sample fluid for producing and providing in-container filled fluid samples for analysis and / or limit monitoring
CN106222083A (en) * 2016-08-18 2016-12-14 袁宇志 Intelligent pair of gas culture tank control system

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102015007049A1 (en) * 2015-05-29 2016-12-01 Lukas Neuhaus Method with an electronic sample fluid receiver for producing and providing fluid samples filled in environmentally sealed containers for analysis and / or limit monitoring
DE102015007050A1 (en) * 2015-05-29 2016-12-01 Lukas Neuhaus Method with an electronic switching valve-based sample fluid for producing and providing in-container filled fluid samples for analysis and / or limit monitoring
DE102015007050B4 (en) 2015-05-29 2023-08-03 Lukas Neuhaus Method with an electronic switching valve-based sample fluid collector for generating and providing fluid samples filled in containers for analysis and/or limit value monitoring
CN106222083A (en) * 2016-08-18 2016-12-14 袁宇志 Intelligent pair of gas culture tank control system

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