CN202347019U - Anaerobic and microaerophilic gas treating device - Google Patents

Anaerobic and microaerophilic gas treating device Download PDF

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Publication number
CN202347019U
CN202347019U CN 201120526938 CN201120526938U CN202347019U CN 202347019 U CN202347019 U CN 202347019U CN 201120526938 CN201120526938 CN 201120526938 CN 201120526938 U CN201120526938 U CN 201120526938U CN 202347019 U CN202347019 U CN 202347019U
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gas
culture tank
cpu
pressure
control system
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刘嘉蓉
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GUANGZHOU HITECH PHOTOELECTRICITY BIOTECH CO Ltd
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GUANGZHOU HITECH PHOTOELECTRICITY BIOTECH CO Ltd
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    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M45/00—Means for pre-treatment of biological substances
    • C12M45/02—Means for pre-treatment of biological substances by mechanical forces; Stirring; Trituration; Comminuting
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/40—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M45/00—Means for pre-treatment of biological substances
    • C12M45/20—Heating; Cooling

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Abstract

The utility model discloses an anaerobic and microaerophilic gas treating device, comprising a main control system, a culture tank and a mixed gas source, wherein the main control system is respectively connected the culture tank and the mixed gas source through a tank body connecting pipe and a gas source connecting pipe; a vacuum pump, a control circuit electrically connected with the vacuum pump, a pressure sensor or a CO2 sensor, a mixed gas inlet electromagnetic valve arranged on the gas source connecting pipe, a vacuum electromagnetic valve and a central processing unit electrically connected with the control circuit are arranged in the main control system, and the control circuit is respectively electrically connected with the mixed gas inlet electromagnetic valve and the control vacuum electromagnetic valve; one end of the pressure sensor is respectively connected with the tank body connecting pipe and the culture tank, the other end of the pressure sensor is electrically connected with the central processing unit and used for detecting gas supply pressure of the mixed gas source, pressure of the mixed gas in the culture tank and gas tightness for connection of the culture tank and the main control system, and then transmitting detection signals to the central processing unit. The whole device has the advantages of high precise control degree, high reliability, good impermeability, and better repeatability of results.

Description

A kind of anaerobism, little aerobic gas treatment equipment
Technical field
The utility model relates to the self-reacting device equipment technical field of separating and cultivating anerobes and microaerobe.
Background technology
To manipulation requires such as the separation of microaerobe and anerobes, cultivations the particular environment condition is provided in the bacteriology.Based on the needs of cultivating this quasi-microorganism; Various robotization anaerobism in the market or little aerobic operation device such as products such as anaerobism or little aerobic cultivation operation box, complex equipments involves great expense; Bulky; And exist gas consumption big, use cost is high, reaches shortcomings such as anaerobic environment condition length consuming time.
The utility model content
The purpose of the utility model has been to overcome the weak point of existing anaerobism on the market, the operation of little oxygen and culture apparatus, for the Experiment on Microbiology worker provide a kind of equipment simple, easy and simple to handle, form the intelligent automation cultivating operating apparatus of anaerobic or little aerobic environment fast.
For realizing above purpose; Technical scheme below the utility model has been taked: a kind of anaerobism, little aerobic gas treatment equipment; Include main control system, culture tank, gas mixture source of the gas, this main control system is connected with the gas mixture source of the gas with culture tank respectively through tank body pipe connecting, source of the gas pipe connecting; In said main control system, be provided with vacuum pump, the pilot circuit that is electrically connected with vacuum pump, pressure transmitter or CO 2Transmitter, be arranged on the mixed gas air inlet SV on the source of the gas pipe connecting, the cpu that vacuumizes SV, is electrically connected with pilot circuit, pilot circuit vacuumizes SV and is electrically connected with said mixed gas air inlet SV, control respectively; Said pressure transmitter one end is connected with tank body pipe connecting, culture tank respectively, and the other end is electrically connected with cpu, and it is used to detect the supply gas pressure of gas mixture source of the gas, mixture pressure or the CO in the culture tank 2The resistance to air loss that gas concentration and culture tank are connected with main control system, and send detection signal to cpu;
When carrying out little oxygen operation sequence, pressure sensor detects the supply gas pressure of source of the gas and the gas pressure in the culture tank respectively, and detected value is sent to central processing unit, and central processing unit is based on preset value and detected value contrast, if be not inconsistent, work is ended; If conform to; Then carry out following gas pump drainage replacement process: central processing unit sends the Different control signal respectively and gives control circuit, control circuit based on this control signal control respectively mist air inlet magnetic valve by, control vacuumize solenoid valve conduction, the control vavuum pump starts; Pressure sensor continues to detect the negative pressure in the culture tank; And detected value sent to central processing unit; When this detected value when predetermined value in the central processing unit equates; Then central processing unit sends the Different control signal and gives control circuit, control circuit based on this control signal control respectively vavuum pump stop, control vacuumize magnetic valve by, control mist air inlet solenoid valve conduction; When central processing unit receives detected pressures value that pressure sensor transmits when conforming to standard atmospheric pressure; Central processing unit sends control signal and gives control circuit; Control circuit ends based on this control signal control mist air inlet magnetic valve, and said process is a gas pump drainage displacement;
When carrying out the anaerobic operation program, said pressure transmitter detects the gaseous tension in the culture tank, and detected value is sent to cpu, and cpu is according to preset value and the contrast of this detected value, if be not inconsistent, work is ended; If conform to, then repeat above-mentioned three times gas pump drainage replacement process.
In the actual tests process of anaerobic operation; Detected value in the anaerobic operation program and preset value respectively with little oxygen schedule of operation in detected value and preset value identical; Just being provided with of cpu can be top described at least two to three repetitive operations in the anaerobic operation program, and the experimenter can select little aerobic operation program voluntarily or the anaerobic operation program makes an experiment.
The intelligent anaerobism of this kind/little aerobic gas treatment equipment; In conjunction with traditional " catalyzer oxygen consumption technology " and " gas pump drainage replacement technique "; And adopt intelligence software robotization red-tape operati program, can reach the desirable culture environment of cultivating anerobes or microaerobe.If select little oxygen schedule of operation; Then the built-in accurate vacuum pump of system host is operated through the pilot circuit sequence of control; Will with culture tank that main frame is connected in contain the oxygen air and go out by the respective line pump drainage after, (be generally 80%N by the mixed gas that source of the gas provided that is connected with main frame again 2, 10%CO 2, 10%H 2Thereby) be fills up to and reach little aerobic environment in the culture tank; If select the anaerobism program, then above operating process triplicate, remaining trace oxygen reaches the required oxygen-free environment condition of anerobes in the jar thereby exhaust through the palladium grain catalysis of presetting in the culture tank then;
The design of full-automatic control pump drainage gas gas ratio: anaerobism/little aerobic gas treatment equipment and culture tank are linked the tank body interface of main frame through quick vacuum breaker plug; Through the self-defined suction pressure of intelligent software; Main frame is surveyed the gaseous tension in the culture tank through precision pressure sensor; The pressure result of detection is fed back to cpu; Cpu is carried out corresponding actions according to pre-set programs to pilot circuit and two electromagnetic valve components, thereby realizes gas in the culture tank and the mixed gas in the source of the gas are carried out pump drainage displacement automatically apace; The user can be according to the component ratio of experiment demand gaseous mixture self-defined to be imported; Control instruction through automated software; Cooperate the mixed gas in the source of the gas of selecting preparation in advance to reach needed component ratio; And then make with culture tank that device links to each other in reach the desired level of user after the pump drainage displacement of oxygen concentration through certain number of times, satisfy the requirement of various gas culture condition;
With the little oxygen program of standard is example; This program is for micro-aerobic bacteria optimum culture condition to be provided; The account form of oxygen remaining proportion is in the little oxygen menu of standard in software program: primary oxygen concentration is 21% in the culture tank, and is consistent with oxygen concentration in the air.Little good oxygen condition generally is meant the condition that needs 6% oxygen concentration, and this just means that 100-in the initial environment (6/21 * 100)=71.42858% gas concentration must replace with the mixed gas of no oxygen.For reaching this state, to calculate with initial 1 standard atmospheric pressure, the little oxygen program of standard pump drainage vacuum calculates final filling point (being about 1013mbar) then to 286mbar, then replaces filling with the oxygenless gas mixture that is connected.This anaerobism/little aerobic system can highly precisely accomplish aforesaid operations automatically;
Standard anaerobism program then is for anerobes optimal culture condition to be provided; In standard program, accomplish and carry out three replacement cycles; The calculating of oxygen remaining proportion is in the standard anaerobism menu: primary oxygen concentration is 21% in the culture tank, is consistent with oxygen concentration in the air; In each circulation culture tank, surpass 80% gas approximately and replaced (pump drainage to 200mbar recharge) by the oxygenless gas mixture to 1013mbar.After three replacement cycles, only remain in jar (1-80.26%) 3* 21%=0.16% oxygen.
The pressure transmitter of the utility model also has full device resistance to air loss detection effect: when handling each culture tank; This device can require to carry out the full device resistance to air loss and detect according to the Quality Control that user's software operation instruction set, comprise gas leak-testing under A, the high vacuum state: detect this device automatically and be in culture tank that deeper vacuum state following time ought a certainly be connected and occur jar revealing a test crash and show that then there is leakage problem in this culture tank.B, the airtight leak-testing of low vacuum state: ACOE is near state under the barometric point and shows then that when the seal leakage test crash appears in a certain continuous culture tank there is leakage problem in this device down; Be connected test respectively with culture tank through the main frame precision pressure sensor, guarantee that the full device air-tightness is reliable, 100% does not have leakage.
Through central processing unit, the predeterminable S.O.P. of the utility model device, the user can directly cultivate the standardization program that gas condition directly selects for use system to prestore based on different microorganism species or difference; Also can be, and can store self defining programm based on concrete experiment demand through touch-screen or the self-defined required pressure of computer operation, gas component, Quality Control level etc., output experiment condition setting or print operation.
When carrying out the anaerobic operation program, in said culture tank, be placed with deoxidation catalyst in advance.In conjunction with deoxidation catalyst, can thoroughly remove the residual oxygen in the culture tank.
When carrying out the cell cultures schedule of operation, the user can select to open CO 2The concentration measuring ability, system host can pass through CO 2Transmitter is surveyed the CO in the culture tank 2Gas concentration is with CO 2The concentration result of detection feeds back to cpu, and cpu is carried out corresponding actions according to pre-set programs to pilot circuit and two electromagnetic valve components, thereby realizes gas in the culture tank and the mixed gas in the source of the gas are carried out pump drainage displacement automatically apace; The user can be according to the component ratio of experiment demand gaseous mixture self-defined to be imported; Control instruction through automated software; Concentration of carbon dioxide reaches needed component ratio in the mixed gas in the source of the gas that cooperation is selected to prepare in advance; And then make with culture tank that device links to each other in reach the desired level of user after the pump drainage displacement of density of carbon dioxide gas through certain number of times, satisfy the requirement of various gas culture condition.
On said main control system, also be provided with USB interface, this USB interface is electrically connected with said cpu.Make hardware and software upgrading convenient feasible through USB interface: the user can select for use the different volumes culture tank directly then can arbitrarily increase gas interface to 3 or increase tank body with device coupling, HardwareUpgring arbitrarily based on the real work amount and be interfaced to 3, and directly carries out the corresponding software upgrading through USB interface.
Also include the touch display screen that is electrically connected with cpu, each detected value that this cpu will receive from pressure transmitter and the running status of main control system are shown on the touch display screen.Main frame can detect the working order of each several part automatically in the entire operation process; Possesses the gaseous tension measuring ability; And can show error situation and detected result of jar pressure condition of interior gas pump drainage, operating process etc. in real time; Real-time false alarm and in video display, operating result repeatable 100%; Main frame or the monitor showing at any time of culture tank internal pressure, easy to use and reliable.
On said culture tank, also be connected with the culture tank SV, this culture tank SV is connected to the gas bar, and the said gas bar the other end is connected with said pressure transmitter, said gas bar also respectively with mixed gas air inlet SV, vacuumize SV and be connected.Pressure transmitter detects the supply gas pressure of gas mixture source of the gas, promptly is equivalent to detect the gas mixture source of the gas and is punched into the mixture pressure in the gas bar.
The intelligent anaerobism of the utility model, little aerobic gas treatment equipment belong to the manufacturing and the Application Areas of the self-reacting device equipment of mikrobe separation and cultivation.This device is made up of main control system, cpu, gas circuit and circuit control system; It is a kind of self-reacting device device that adopts gas pump drainage principle,displacement; Its built-in accurate oilless vacuum pump and wiring; Automatically accomplish the pump drainage and the displacement of gas through cpu; Make in the cultivation tank body that is connected and reach predetermined atmosphere surrounding fast; Can be each culture tank provides and independently cultivates gas condition, i.e. anaerobism culture system, little aerobic culture system or user-defined mixed gas culture system etc., thus carry out the experimental installation that experimental studies such as separation and Culture anaerobism and microaerophile provide a kind of intelligence, robotization for Microbiological Lab; This device adopts the microcomputer-based intelligent function software; Entire operation process with touch-screen or computer interconnection operating method main control system; Gas concentration such as the oxygen that anaerobism, little aerobic or User Defined is required through the standard program menu, carbonic acid gas, hydrogen and culture tank internal pressure are carried out precision; Can programme to the mixing gas component of varying environment condition and define regulation and control; Automatically for each culture vessel, every kind of cultivation provide different atmosphere surrounding conditions separately; Realize that start starts at once, full-automatic control panel is operated, the quick conversion of different operating condition need not to wait for; The intelligence operation software function is abundant, handiness is high, user interface is friendly and upgrading is convenient, can be widely used in the corresponding anerobes of industries such as medical treatment, disease control, medicine, scientific research, food and environmental protection, little aerobic bacteria, cell cultures etc., for further identifying, separating effective assurance is provided.
The utility model compared with prior art has following advantage:
1. whole device adopts the intelligent software operation, and accurate control degree is high, safety is high, good leak tightness, result have better repeatability; Cpu is through the software control circuit, and precision is high, and good stability uses reliably, economizes gas, economy, operational safety, dependable performance;
2. simple operation, highly effective and safe, can fully realize individual operation; Whole gas path device is through software and solenoid control, and gaseous fraction is provided with flexibly, and control accuracy is good
3. entire operation process dried and clean, no crossed contamination, no chemical waste, safety and environmental protection;
4. air consumption is low, need not large-scale gas-holder, running cost is low;
5. compact construction is convenient to the systematize operation; Can arbitrarily increase gas or tank body communication port according to customer need, the autonomous experiment of real realization is provided with; The equipment subsequent upgrade is also extremely convenient;
6. experimental arrangement and data can realize storage or print;
7. Maintenance free maintenance;
8. the scope of application is extensive: both can be used for anaerobic operation, little aerobic operation, also can be used for the cell cultures operation;
Cost performance high, be easy to promote the popularization use.
Description of drawings
Fig. 1 is the utility model anaerobism, little aerobic gas treatment equipment schematic block diagram;
Fig. 2 is the utility model anaerobism, little aerobic gas treatment equipment hardware block diagram;
Description of reference numerals: 1-vacuum pump, 2-pilot circuit, 3-pressure transmitter or CO 2Transmitter, 4-mixed gas air inlet SV, 5-vacuumizes SV, 6-operating display, 7-tank body pipe connecting, 8-source of the gas pipe connecting, 9-cpu, 20-main control system, 21-culture tank, 211-culture tank SV, 22-gas mixture source of the gas, 23-gas bar.
Embodiment
Explain further details below in conjunction with accompanying drawing and embodiment content to the utility model.
Embodiment:
See also illustrated in figures 1 and 2; This anaerobism, little aerobic gas treatment equipment are to be applied to the apparatus that Microbiological Lab carries out gas pump drainage displacement purposes; Include main control system 20, culture tank 21, gas mixture source of the gas 22, be arranged on mixed gas air inlet SV 4 on the source of the gas pipe connecting 8, vacuumize SV 5; This main control system 20 through tank body pipe connecting 7, source of the gas pipe connecting 8 be connected with gas mixture source of the gas 22 with culture tank 21 respectively, its main control system 20 built-in accurate oilless vacuum pumps 1; And the pilot circuit 2 that is electrically connected with vacuum pump 1, pilot circuit 2 vacuumizes SV 5 and is electrically connected with mixed gas air inlet SV 4, control respectively; Pressure transmitter 3 one ends are connected with tank body pipe connecting 7, culture tank 21 respectively; The other end is electrically connected with cpu 9; It is used to detect the resistance to air loss that mixture pressure and culture tank 21 in the supply gas pressure, culture tank 21 of gas mixture source of the gas 22 are connected with main control system 20, and sends detection signal to cpu 9; Culture tank 21 through cpu 9, pressure transmitter 3, gas circuit and pilot circuit with pilot circuit 2 is electrically connected in the main control system 20 come main control system 20 is connected is carried out the displacement of gas pump drainage, about 1 minute of 1 circulation required time of gas pump drainage displacement.The operator can choose through operating display or self-defined mixing gas component as required; Select or self-defined anaerobism or little aerobic program start; Behind the program start; Cpu 9 is through pressure transmitter 3 and gas mixing ratio, and the suction air pressure that calculating needs, the steering routine and the working cycle number of times that need are carried out corresponding quality guarantee and gas replacement program successively.
Gas mixture source of the gas 22, culture tank 21 are connected with culture tank tank body interface with the gas source interface of main control system 20 respectively; After confirming the schedule of operation menu through touch display screen 6 inputs; Press the starting switch of main frame; Cpu 9 control pressure transmitters 3 detect culture tank 21 internal pressures, and pilot circuit 2 is further controlled respective electrical magnet valve and vacuum pump work according to pressure change then, thereby realize jar pump drainage and the displacement of interior gas.
Concrete working process is following:
After main control system 20 is connected, select the program of required execution as required through touch display screen 6 operations.If select to carry out little aerobic program, then behind the start-up routine, after the host-initiated program; Cpu 9 sends control signal corresponding and gives pilot circuit 2; Pilot circuit 2 is controlled 4 conductings of mixed gas air inlet SV respectively, control vacuumizes SV 5 and ends, and pressure transmitter 3 detects the leak detection that is connected of mixture pressures and culture tank 21 and main control system 20, and detected result is fed back to cpu 9; If undesired, cease operation; If each item index is that detected value is all normal with settings; Then carry out following gas pump drainage replacement process: cpu 9 sends the Different control signal respectively and gives pilot circuit 2; Pilot circuit 2 is controlled vacuum pump 1 respectively according to this wave and is started, and vacuum solenoid 5 conductings, control mixed gas air inlet SV 4 end, and pressure transmitter 3 continues to detect the negative pressure in the culture tank 21; And detected value sent to cpu 9; When this detected value when preset value in the cpu 9 equates, then cpu 9 sends the Different control signal and gives pilot circuit 2, pilot circuit 2 according to this wave control respectively vacuum pump 1 stop, control vacuumize SV 5 by, control 4 conductings of mixed gas air inlet SV; Mixed gas charges into culture tank 21; When culture tank 21 internal pressures reached standard atmospheric pressure, mixed gas was filled and is accomplished, and the displacement of gas pump drainage finishes; When cpu 9 receives detecting pressure value that pressure transmitter 3 transmits when conforming to standard atmospheric pressure; Cpu 9 sends wave and gives pilot circuit 2; Pilot circuit 2 ends according to this wave control mixed gas air inlet SV 4, and main control system 20 is returned to original state, and said process is a gas pump drainage displacement; Make and keep little oxygen environment to get final product in the culture tank 21; But if the oxygen content baric minimum very little or that can bear that requires is bigger, then can repeatedly replace or use instead self defining programm, carry out experimental implementation behind the preset values such as setting oxygen desired content and minimum suction pressure;
If select to carry out the anaerobic operation program, the operator is through the anaerobism programs menu start-up routine of the required execution of touch display screen 6 operation selections, and cpu 9 at first carries out pressure detection through pressure transmitter 3, if undesired, cease operation; If it is normal; Pilot circuit 2 repeats above-mentioned three gas pump drainages displacement, also can in advance deoxidation catalyst be put into culture tank 21, impels culture tank 21 interior remnant oxygen to exhaust by deoxidation catalyst; Cause 100% anaerobic state in the culture tank, then the anaerobic jar gas displacement is accomplished.
The present embodiment gas treatment equipment also possesses the Quality Control design: device between locomorphic stage, can be selected the Quality Control level of different stage at the gas pump drainage according to user's different experimental conditions demand voluntarily.Carry out the test of standard source of the gas gas input pressure respectively through pressure transmitter; Culture tank connects test; Seal leakage test, catalyst activity property testing (be applicable under the absolute anaerobic culture environment condition and select for use under the situation of catalyzer) etc.; Thereby guarantee that whole device reaches that the full device airtight performance is normal, each building block its functional attributes all meets quality control standard; Finally make the interior atmosphere surrounding condition of culture tank satisfy required condition and guarantee that tank body does not have gas leak between during cultivation, experimental implementation reproducibility of results 100%.
The operation of full automatic control screen reaches the anaerobic environment required time: 5 minutes (comprising steady time); Reach little oxygen environment required time: 2 minutes (comprising steady time); And different operating condition conversion fast need not to wait for the function software feature richness.
In the present embodiment; Pilot circuit 2 is available circuits; It is used for IO signal with cpu 9 output and is amplified to and can controls each electromagnetic valve switch and vacuum pump switch (it controls each SV through the control solenoid), adopts general amplifying circuit to add the rly. realization.
ADC among Fig. 2 (Analog-to-Digital Conventer) is an analog, is prior art, and it is used for the simulating signal dress of pressure transmitter 3 outputs is changed to numerary signal, so that cpu 9 is handled; On culture tank 21, also be connected with culture tank SV 211, it is used for switch culture tank 21, and this culture tank SV 211 is connected to gas bar 23; Gas bar 23 the other ends are connected with pressure transmitter 3, gas bar 23 also respectively with mixed gas air inlet SV 4, vacuumize SV 5 and be connected, when culture tank SV 211 is opened; 21 gentle 23 of above-mentioned culture tank are communicated with; Gas bar 23 is a kind of gaseous interchange cavitys, thus culture tank 21 can with other parts swap gas that is communicated with gas bar 23 equally, as bleeding by vacuum pump 1; Perhaps, when valve open, get final product mutual exchanging gas by 22 inflations of gas mixture source of the gas.
Two-wire among present embodiment Fig. 1 is connected to gas circuit and connects, and the circuit that is connected to of single line band arrow connects, and the gas circuit that is connected to of two-wire band arrow connects among Fig. 2, and the circuit that is connected to of single line band arrow connects.
Above-listed detailed description is to the specifying of the utility model possible embodiments, and this embodiment is not the claim in order to restriction the utility model, does not allly break away from the equivalence that the utility model does and implements or change, all should be contained in the claim of this case.

Claims (5)

1. an anaerobism, little aerobic gas treatment equipment; It is characterized in that: include main control system (20), culture tank (21), gas mixture source of the gas (22), this main control system (20) is connected with gas mixture source of the gas (22) with culture tank (21) respectively through tank body pipe connecting (7), source of the gas pipe connecting (8); The pilot circuit (2), pressure transmitter or the CO that in said main control system (20), be provided with vacuum pump (1), are electrically connected with vacuum pump (1) 2Transmitter (3), be arranged on the mixed gas air inlet SV (4) on the source of the gas pipe connecting (8), the cpu (9) that vacuumizes SV (5), is electrically connected with pilot circuit (2), pilot circuit (2) vacuumizes SV (5) and is electrically connected with said mixed gas air inlet SV (4), control respectively;
Said pressure transmitter or CO 2Transmitter (3) one ends are connected with tank body pipe connecting (7), culture tank (21) respectively, and the other end is electrically connected with cpu (9), and it is used to detect the supply gas pressure of gas mixture source of the gas (22), mixture pressure or the CO in the culture tank (21) 2The resistance to air loss that gas concentration and culture tank (21) are connected with main control system (20), and send detection signal to cpu (9);
Pressure transmitter (3) detects the supply gas pressure of gas mixture source of the gas (22) and the gaseous tension in the culture tank (21) respectively, and detected value is sent to cpu (9), and cpu (9) is according to preset value and detected value contrast, if be not inconsistent, work is ended; If conform to; Cpu (9) sends the Different control signal respectively and gives pilot circuit (2), pilot circuit (2) according to this wave control respectively mixed gas air inlet SV (4) by, control vacuumize SV (5) conducting, control vacuum pump (1) starts; Pressure transmitter (3) continues to detect the negative pressure in the culture tank (21); And detected value sent to cpu (9); When this detected value when preset value in the cpu (9) equates; Then cpu (9) sends the Different control signal and gives pilot circuit (2), pilot circuit (2) according to this wave control respectively vacuum pump (1) stop, control vacuumize SV (5) by, control mixed gas air inlet SV (4) conducting; When cpu (9) receives detecting pressure value that pressure transmitter (3) transmits when conforming to standard atmospheric pressure; Cpu (9) sends wave and gives pilot circuit (2), and pilot circuit (2) ends according to this wave control mixed gas air inlet SV (4).
2. anaerobism as claimed in claim 1, little aerobic gas treatment equipment is characterized in that: when carrying out the anaerobic operation program, in said culture tank (21), be placed with deoxidation catalyst in advance.
3. anaerobism as claimed in claim 1, little aerobic gas treatment equipment is characterized in that: on said main control system (20), also be provided with USB interface (23), this USB interface (23) is electrically connected with said cpu (9).
4. like arbitrary described anaerobism, little aerobic gas treatment equipment in the claim 1 to 3; It is characterized in that: also include the touch display screen (6) that is electrically connected with cpu (9), each detected value that this cpu (9) will receive from pressure transmitter (3) and the running status of main control system (1) are shown on the touch display screen (6).
5. anaerobism as claimed in claim 4, little aerobic gas treatment equipment; It is characterized in that: on said culture tank (21), also be connected with culture tank SV (211); This culture tank SV (211) is connected to gas bar (23); Said gas bar (23) the other end is connected with said pressure transmitter (3), said gas bar (23) also respectively with mixed gas air inlet SV (4), vacuumize SV (5) and be connected.
CN 201120526938 2011-12-14 2011-12-14 Anaerobic and microaerophilic gas treating device Expired - Fee Related CN202347019U (en)

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CN 201120526938 CN202347019U (en) 2011-12-14 2011-12-14 Anaerobic and microaerophilic gas treating device

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CN 201120526938 CN202347019U (en) 2011-12-14 2011-12-14 Anaerobic and microaerophilic gas treating device

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