CN200949107Y - Portable automatic anaerobic/oxygen micro-need culturing device - Google Patents

Portable automatic anaerobic/oxygen micro-need culturing device Download PDF

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Publication number
CN200949107Y
CN200949107Y CN 200620064559 CN200620064559U CN200949107Y CN 200949107 Y CN200949107 Y CN 200949107Y CN 200620064559 CN200620064559 CN 200620064559 CN 200620064559 U CN200620064559 U CN 200620064559U CN 200949107 Y CN200949107 Y CN 200949107Y
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gas
interface
source
magnetic valve
electromagnetic valve
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CN 200620064559
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杨光世
刘嘉蓉
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/42Integrated assemblies, e.g. cassettes or cartridges
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/54Constructional details, e.g. recesses, hinges hand portable
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/40Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
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  • Microbiology (AREA)
  • Sustainable Development (AREA)
  • Biotechnology (AREA)
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  • General Engineering & Computer Science (AREA)
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  • Clinical Laboratory Science (AREA)
  • Analytical Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

A potable automatic anaerobic/ microaerophilic culture device comprises a standard mixed-air supplier, a culture jar and a control mainframe. The mainframe includes an oilless vacuum pump, an air supplier electromagnetic valve, a vaccumized electromagnetic valve, a pressure sensor, a vacuum display list and a MPU control circuit. The standard mixed-air supplier goes through the air supplier connecting tube and is connected with the air supplier electromagnetic valve via the air supplier interface; the oilless vacuum pump is connected with the vacuumized electromagnetic valve; the air supplier electromagnetic valve and the vaccumized electromagnetic valve are connected with the culture jar via the air-replacement interface; the pressure sensor is equipped in the air passage accessing the culture jar; the MPU control circuit produces program control signals for the air supplier electromagnetic valve, the vaccumized electromagnetic valve and the oilless vacuum pump based on the pressure signal input by the pressure sensor to control the air pumping drainage and replacement in the culture jar. This system has the advantages of high efficiency and utility, portability and small volume.

Description

Portable Automatic anaerobism/little aerobic culture apparatus
Technical field
The utility model relates to a kind of device that is used to separate and cultivate anerobe and microaerobe.
Background technology
Provide the particular environment condition to manipulation requires such as the separation of microaerobe and anerobe, cultivations in the bacteriology.Based on the needs of cultivating this quasi-microorganism, various automatization anaerobism in the market or little aerobic operation device such as products such as anaerobism or little aerobic cultivation operation box, the equipment complexity, involve great expense, bulky, and exist gas consumption big, the use cost height reaches shortcomings such as anaerobic environment condition length consuming time.
Summary of the invention
The purpose of this utility model is the many weak points at existing anaerobism or little aerobic operation device on the market, propose a kind of equipment simple, easy and simple to handle, to Portable Automatic anaerobism/little aerobic culture apparatus of quick formation anaerobic or little aerobic environment.
The purpose of this utility model realizes by technical scheme: designed Portable Automatic anaerobism/little aerobic culture apparatus is by the normal mixture source, culture tank and main control system constitute, described main control system includes oilless vacuum pump, the source of the gas magnetic valve, vacuumize magnetic valve, pressure transmitter, vacuum tightness indicator gauge and microprocessor control circuit, and above main control system, be provided with gas source interface and gas displacement interface, described normal mixture source is connected with gas source interface on the main control system by the source of the gas pipe connecting, this gas source interface is connected to the input interface of source of the gas magnetic valve, oilless vacuum pump is connected with the output interface that vacuumizes magnetic valve, the output interface of source of the gas magnetic valve and the input interface that vacuumizes magnetic valve are connected to the gas displacement interface on the main control system, this gas displacement interface is connected to the fast interface of culture tank by quick connecting pipe, pressure transmitter is installed in the gas circuit that inserts culture tank, the vacuum tightness indicator gauge is connected with pressure transmitter, the pressure signal input microprocessor pilot circuit that pressure transmitter produces, microprocessor control circuit produces the source of the gas magnetic valve according to the pressure signal of being imported, vacuumize the program control signal of magnetic valve and oilless vacuum pump, the gas pump drainage and the displacement of control culture tank.
Can the culture tank stationary installation be set in described main control system one side, so that place the tank body on-line operation.
This Portable Automatic anaerobism/when little aerobic culture apparatus is worked, the normal mixture bottle is connected with the gas source interface of main control system, and culture tank is connected with the gas displacement interface of main control system, behind the starting switch of pressing main control system, pressure transmitter detects the culture tank internal pressure, the pressure signal input microprocessor pilot circuit that is produced, microprocessor control circuit is further controlled respective electrical magnet valve and oil-free vacuum pump work according to this pressure change, thereby realizes the pump drainage and the displacement of gas in the culture tank.
This Portable Automatic anaerobism/little aerobic culture apparatus is to be applied to the apparatus that Microbiological Lab carries out gas pump drainage displacement purposes, its built-in accurate oilless vacuum pump, this device comes the culture tank that device is connected is carried out the displacement of gas pump drainage by the microprocessor in the main control system, pressure transmitter and pilot circuit, about 50 seconds of 1 circulation required time of gas pump drainage displacement.If need provide the anaerobism culture condition then under the jar inner cap, to fold up the palladium catalyst grain, will carry out gas pump drainage displacement back jar interior residual oxygen reaction approach exhaustion 3 times, cause jar interior 100% anaerobic state; If need provide little aerobic culture condition then only gas in the jar to be carried out 1 pump drainage displacement, jar little oxygen environment of interior maintenance is got final product.
Positively effect of the present utility model has been to overcome the shortcoming and defect part of present various commercial anaerobism/little oxygen operation and culture apparatus; This device possesses following advantage: 1. device is desk-top portable design, convenient light and handy; 2. compact construction is convenient to the device operation; 3. its entire operation process dried and clean, no crossed contamination, no chemical waste; 3. easy and simple to handle, highly effective and safe and environment friendly and pollution-free 4. can accurate controls, reliability height, good reproducibility as a result; Air consumption low, need not large-scale gas-holder, running cost is low; 6. install easy maintaining; 7. the scope of application is extensive, can be used for anaerobic operation and also can be used for little aerobic operation; The cost performance height, be easy to promote the popularization use.
Description of drawings
Fig. 1 is the formation synoptic diagram of this device;
Fig. 2 is this apparatus structure skeleton diagram;
Fig. 3 is the microprocessor control circuit figure of this device.
Embodiment
Below in conjunction with accompanying drawing being described in further detail to embodiment of the present utility model:
As shown in Figure 1, this Portable Automatic anaerobism/little aerobic culture apparatus is made of normal mixture source 1, culture tank 2 and main control system 3, above main control system, be provided with gas source interface 10, gas displacement interface 11 and portable handle 16, the operating panel of main control system is provided with vacuum tightness indicator gauge 8, also have start button switch 14 and functional select switch 15, normal mixture source 1 is connected with gas source interface 10 on the main control system 3 by source of the gas pipe connecting 12, and the gas displacement interface 11 on the main control system 3 is connected to the fast interface of culture tank 2 by quick connecting pipe 13.Main control system 3 is built-in with oilless vacuum pump 4, source of the gas magnetic valve 5, vacuumize magnetic valve 6, pressure transmitter 7, microprocessor control circuit 9 and pipe installation, Fig. 2 has shown the annexation between each parts, normal mixture source 1 is connected to the input interface (through gas source interface 10) of source of the gas magnetic valve 5, oilless vacuum pump 4 is connected with the output interface that vacuumizes magnetic valve 6, the output interface of source of the gas magnetic valve 5 and the input interface that vacuumizes magnetic valve 6 are connected to the fast interface (through gas displacement interface 11) of culture tank 2, pressure transmitter 7 is installed in the gas circuit that inserts culture tank 2, vacuum tightness indicator gauge 8 is connected with pressure transmitter 7, microprocessor control circuit 9 produces source of the gas magnetic valve 5 according to the pressure signal of pressure transmitter 7 inputs, vacuumize the program control signal of magnetic valve 6 and oilless vacuum pump 4, the gas pump drainage and the displacement of control culture tank 2.
Microprocessor control circuit 9 adopts the control core of PIC16C57 micro-chip as circuit assembly.The basic hardware schematic circuit as shown in Figure 3.In this circuit, RA0~RA3 is used for seven segment code and shows, RB6 source of the gas solenoid control signal, RB7 vacuum pump solenoid control signal, RB7 vacuumizes the solenoid control signal, and RC3~RC5 is used for the function button design, and RC0~RC2 is used for carrying out pressure survey with sensor communication, RB0~RB1 scans seven segment code, and RB2~RB4 LED light shows.
According to the pre-set programs of microprocessor control circuit 9, the concrete working process of this automatic anaerobism/little aerobic culture apparatus is as follows:
Connect host power switch, the selection function switch 15 as required, if carry out little aerobic operation, press start button 14, device at first carries out pressure detection: 5 conductings of microprocessor control circuit 9 control source of the gas magnetic valves, vacuumize magnetic valve 6 and end, pressure transmitter 7 examination criteria bleed pressures and culture tank connect leak detection, and detected result is fed back to microprocessor control circuit 9.If undesired, cease operation; If it is normal, microprocessor control circuit 9 control oilless vacuum pumps 4 start, vacuumize magnetic valve 6 conductings simultaneously, source of the gas magnetic valve 5 ends, after negative pressure reaches standard in the pressure transmitter 7 detection culture tank, oilless vacuum pump 4 quits work, vacuumize magnetic valve 6 simultaneously and end, 5 conductings of source of the gas magnetic valve, standard gas charges into culture tank 2, when the culture tank internal pressure reached standard atmospheric pressure, standard gas was filled and is finished.Source of the gas magnetic valve 5 ends simultaneously, and oilless vacuum pump 4 quits work, and vacuumizes magnetic valve 6 and ends, and device is got back to original state.If selection function switch 15 carries out anaerobic operation, then need earlier catalyzer to be put into culture tank, press start button 14, device at first carries out pressure detection, if undesired, cease operation; If normal, microprocessor control circuit 9 repeats above-mentioned three gas pump drainages displacement, impels a jar interior remnant oxygen to exhaust by catalyzer afterwards.The anaerobic jar gas displacement is finished.

Claims (2)

1. Portable Automatic anaerobism/little aerobic culture systems, it is characterized in that by normal mixture source (1), culture tank (2) and main control system (3) constitute, described main control system (3) includes oilless vacuum pump (4), source of the gas magnetic valve (5), vacuumize magnetic valve (6), pressure transmitter (7), vacuum tightness indicator gauge (8) and microprocessor control circuit (9), and above main control system, be provided with gas source interface (10) and gas displacement interface (11), described normal mixture source (1) is connected with gas source interface (10) on the main control system by source of the gas pipe connecting (12), this gas source interface (10) is connected to the input interface of source of the gas magnetic valve (5), oilless vacuum pump (4) is connected with the output interface that vacuumizes magnetic valve (6), the output interface of source of the gas magnetic valve (5) and the input interface that vacuumizes magnetic valve (6) are connected to the gas displacement interface (11) on the main control system, this gas displacement interface (11) is connected to the fast interface of culture tank (2) by quick connecting pipe (13), pressure transmitter (7) is installed in the gas circuit that inserts culture tank (2), vacuum tightness indicator gauge (8) is connected with pressure transmitter (7), the pressure signal input microprocessor pilot circuit (9) that pressure transmitter (7) produces, microprocessor control circuit (9) produces source of the gas magnetic valve (5) according to the pressure signal of being imported, vacuumize the program control signal of magnetic valve (6) and oilless vacuum pump (4), the gas pump drainage and the displacement of control culture tank (2).
2. Portable Automatic anaerobism according to claim 1/little aerobic culture systems is characterized in that described main control system one side has the stationary installation of culture tank (2).
CN 200620064559 2006-09-21 2006-09-21 Portable automatic anaerobic/oxygen micro-need culturing device Expired - Lifetime CN200949107Y (en)

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Application Number Priority Date Filing Date Title
CN 200620064559 CN200949107Y (en) 2006-09-21 2006-09-21 Portable automatic anaerobic/oxygen micro-need culturing device

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Application Number Priority Date Filing Date Title
CN 200620064559 CN200949107Y (en) 2006-09-21 2006-09-21 Portable automatic anaerobic/oxygen micro-need culturing device

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CN200949107Y true CN200949107Y (en) 2007-09-19

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102161970A (en) * 2011-01-20 2011-08-24 重庆大学 Portable cell incubator
CN108865883A (en) * 2018-06-06 2018-11-23 赵裕光 Observe the device of cell behaviors off field for applying direct current

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102161970A (en) * 2011-01-20 2011-08-24 重庆大学 Portable cell incubator
CN102161970B (en) * 2011-01-20 2013-01-23 重庆大学 Portable cell incubator
CN108865883A (en) * 2018-06-06 2018-11-23 赵裕光 Observe the device of cell behaviors off field for applying direct current
CN108865883B (en) * 2018-06-06 2021-05-07 赵裕光 Device for observing cell biological behaviors under external direct-current electric field

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