CN203794901U - Anaerobic culturing device and system - Google Patents
Anaerobic culturing device and system Download PDFInfo
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- CN203794901U CN203794901U CN201420187436.1U CN201420187436U CN203794901U CN 203794901 U CN203794901 U CN 203794901U CN 201420187436 U CN201420187436 U CN 201420187436U CN 203794901 U CN203794901 U CN 203794901U
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- 238000012258 culturing Methods 0.000 title abstract description 19
- 230000007246 mechanism Effects 0.000 claims abstract description 28
- 239000007789 gas Substances 0.000 claims description 174
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 56
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims description 51
- 238000000605 extraction Methods 0.000 claims description 43
- 229910052757 nitrogen Inorganic materials 0.000 claims description 28
- MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 claims description 21
- 229910001882 dioxygen Inorganic materials 0.000 claims description 21
- 239000000203 mixture Substances 0.000 claims description 21
- 239000001257 hydrogen Substances 0.000 claims description 19
- 229910052739 hydrogen Inorganic materials 0.000 claims description 19
- 239000001569 carbon dioxide Substances 0.000 claims description 17
- 229910002092 carbon dioxide Inorganic materials 0.000 claims description 17
- 235000011089 carbon dioxide Nutrition 0.000 claims description 17
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 claims description 12
- 150000002431 hydrogen Chemical class 0.000 claims description 7
- 238000004891 communication Methods 0.000 claims description 5
- 239000001301 oxygen Substances 0.000 description 8
- 229910052760 oxygen Inorganic materials 0.000 description 8
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 6
- 241001148471 unidentified anaerobic bacterium Species 0.000 description 6
- 238000000034 method Methods 0.000 description 5
- 230000002924 anti-infective effect Effects 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 238000002560 therapeutic procedure Methods 0.000 description 2
- PCTMTFRHKVHKIS-BMFZQQSSSA-N (1s,3r,4e,6e,8e,10e,12e,14e,16e,18s,19r,20r,21s,25r,27r,30r,31r,33s,35r,37s,38r)-3-[(2r,3s,4s,5s,6r)-4-amino-3,5-dihydroxy-6-methyloxan-2-yl]oxy-19,25,27,30,31,33,35,37-octahydroxy-18,20,21-trimethyl-23-oxo-22,39-dioxabicyclo[33.3.1]nonatriaconta-4,6,8,10 Chemical compound C1C=C2C[C@@H](OS(O)(=O)=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2.O[C@H]1[C@@H](N)[C@H](O)[C@@H](C)O[C@H]1O[C@H]1/C=C/C=C/C=C/C=C/C=C/C=C/C=C/[C@H](C)[C@@H](O)[C@@H](C)[C@H](C)OC(=O)C[C@H](O)C[C@H](O)CC[C@@H](O)[C@H](O)C[C@H](O)C[C@](O)(C[C@H](O)[C@H]2C(O)=O)O[C@H]2C1 PCTMTFRHKVHKIS-BMFZQQSSSA-N 0.000 description 1
- 208000035473 Communicable disease Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 125000004435 hydrogen atom Chemical group [H]* 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 244000005706 microflora Species 0.000 description 1
- 210000004400 mucous membrane Anatomy 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses an anaerobic culturing device and system. The anaerobic culturing device comprises a shell body, an air exhausting mechanism and an air inflation mechanism. The air exhausting mechanism is provided with an air exhausting pipe which can be communicated with a culturing tank through an air inlet in the air exhausting pipe and is used for exhausting air in the culturing tank. The air inflation mechanism comprises at least one air inflation pipe which can be communicated with a culturing air source or air through an air inflation inlet in the air inflation pipe. Meanwhile, an air inflation outlet in the air inflation pipe is communicated with a tank body of the culturing tank and is used for the culturing air source or the air to be injected into the culturing tank. By operating a first connecting-disconnecting control piece and a second connecting-disconnecting control piece, air ways in the exhausting pipe and the air inflation pipe are controlled to be connected and disconnected, and by multi-time exhausting-inflation matching, the purpose that a culturing environment is formed in the culturing tank is achieved finally.
Description
Technical field
The application relates to a kind of microbial culture equipment, especially relates to the culture device of a kind of anerobe.
Background technology
Anerobe is distributed widely in each position of nature and Whole Body usually used as normal microflora, on each position of human body, all mucous membrane surfaces have all been covered with anerobe and non-anerobe, and in infectious diseases, anaerobic infection occupies sizable ratio.For a long time, due to the restriction in medical condition and region, the few of anaerobic bacteria culture technology understands in hospital, the equipment that even there is no this respect having, so that clinicist is in the time of anti-infective therapy, can not do anaerobic bacteria culture and test, and form the custom of experience medication, also can affect the effect of anti-infective therapy.
Carrying out anaerobism cultivation is one of big event of hospital's microorganism laboratory, and the method such as useful anaerobic culture box, aerogenesis bag that Hospitals at Present is conventional realizes anaerobism and cultivates.Anaerobic culture box is an airtight large-scale metal box, has culturing room in case, and a transparent panel that side has a synthetic glass to do of culturing room is equipped with two gloves in culturing room on plate, can in culturing room, operate by gloves.There is a switch room in the outside of culturing room, has inside and outside two, and wherein inside door is for isolate forster chamber and switch room, in closing condition.When operation, first will in culturing room, form anaerobic environment, then first open external door, culture is put into switch room.Shut external door and operate and make to reach in switch room anaerobic state, then hand stretches into gloves the inside door of switch room is opened, and article are moved in culturing room, closes upper internal door, maintenance anaerobic state culturing room in.
But there is equipment heaviness, consume the shortcomings such as gas is many, complicated operation, the generation anaerobic environment time is long, equipment volume is difficult to greatly move in existing this anaerobic culture box.
The use of aerogenesis bag is more simple, only medicament (aerogenesis bag) outer packaging need be cut off and is put into container, can form suitable anaerobism, micro-aerobic, carbonic acid gas culture environment after sealing.Its ultimate principle is that the oxygen in enclosed space is completely or partially sponged, and then produces carbonic acid gas.But aerogenesis bag, due to big or small restriction itself, can only be applicable to a certain size spatial dimension, uses very inconvenient.
Summary of the invention
The application provides a kind of culture device for anaerobic bacteria and system.
According to the application's first aspect, the application provides a kind of anaerobic culture device, comprising:
Housing, described housing is that anaerobic culture device provides support;
Extraction sector, described extraction sector is arranged in housing, and it has extraction pipe, and described extraction pipe has the intake interface being communicated with culture tank, and described extraction pipe is provided with first and logical close the logical of gas circuit in control piece control extraction pipe and close;
Also comprise at least one group of inflation mechanism, one group of corresponding at least one culture tank of giving of inflation mechanism is inflated; Described in every group, inflation mechanism comprises at least one gas-filled valve, the inflation outlet that each gas-filled valve has and cultivates the inflation entrance of source of the gas or air communication and is communicated with culture tank, and on each gas-filled valve, be respectively arranged with the second logical close that control piece carries out the interior gas circuit of gas-filled valve logical and close control.
As the further improvement of described anaerobic culture device, described first logical closes control piece and/or second logical to close control piece be machinery valve, and the valve body of described machinery valve is positioned in the gas circuit of extraction pipe or gas-filled valve.
As the further improvement of described anaerobic culture device, described anaerobic culture device also comprises power module, described first logical closes control piece and/or second logical to close control piece be electrically operated valve, described electrically operated valve is electrically connected with power module, and each electrically operated valve is respectively equipped with the break-make of trigger switch control electrically operated valve power source.
As the further improvement of described anaerobic culture device, described anaerobic culture device also comprises controller, described first logical closes control piece and the second logical trigger switch that closes control piece is connected with controller signals, is started and is closed first lead to and close control piece and second and lead to and close control piece by described controller.
As the further improvement of described anaerobic culture device, described in every group, inflation mechanism comprises that at least two for being connected to few two kinds of different gas-filled valves of cultivating source of the gas, described anaerobic culture device also comprises multiple-pass joint, the intake interface of described extraction pipe and the inflation of gas-filled valve outlet are all directly communicated in multiple-pass joint, and described multiple-pass joint direct and culture tank is connected.
As the further improvement of described anaerobic culture device, described anaerobic culture device also comprises pressure transmitter and display module, and described pressure transmitter is arranged at multiple-pass joint for connecting the gas circuit of culture tank.
The application also provides a kind of anaerobism culture systems, comprises anaerobic culture device and at least one culture tank described in above-mentioned arbitrary embodiment; Extraction pipe in described anaerobic culture device is communicated in the tank body of described culture tank, and logical close the logical of gas circuit in control piece control extraction pipe and close by first, gas-filled valve in described inflation mechanism is corresponding to be communicated with the tank body of at least one culture tank, and leads to and close the logical of gas circuit in control piece control gas-filled valve and close by second.
As the further improvement of described anaerobism culture systems, described anaerobism culture systems also comprises cultivation source of the gas, described cultivation source of the gas comprises without oxygen gas mixture, and any in carbon dioxide, nitrogen and three kinds of gases of hydrogen, described inflation mechanism comprises two gas-filled valves, the inflation entrance of one of them gas-filled valve with without oxygen gas mixture connect, any connection in the inflation entrance of another gas-filled valve and carbon dioxide, nitrogen and three kinds of gases of hydrogen.
As the further improvement of described anaerobism culture systems, described anaerobism culture systems also comprises cultivation source of the gas, described cultivation source of the gas comprises carbon dioxide, nitrogen and three kinds of cultivation sources of the gas of hydrogen, described inflation mechanism comprises three gas-filled valves, the inflation entrance of first gas-filled valve and carbon dioxide are connected, the inflation entrance of second gas-filled valve and nitrogen are connected, and the inflation entrance of the 3rd gas-filled valve and hydrogen are connected.
As the further improvement of described anaerobism culture systems, described anaerobism culture systems also comprises cultivation source of the gas, described cultivation source of the gas comprises carbon dioxide, described inflation mechanism comprises two gas-filled valves, the inflation entrance of one of them gas-filled valve is communicated with carbonic acid gas, and the inflation entrance of another gas-filled valve is left a blank.
The application's beneficial effect is:
In the anaerobism culture systems that the application provides, this anaerobic culture device comprises housing, extraction sector and inflation mechanism.This extraction sector has extraction pipe, and extraction pipe can be connected by the intake interface on it and culture tank, for extracting gas in culture tank out.Inflation mechanism comprises at least one gas-filled valve, this gas-filled valve can be by the inflation entrance on it and cultivation source of the gas or air communication, inflation outlet on it is simultaneously communicated with the tank body of culture tank, cultivate source of the gas or air for being filled with in culture tank, logical close control piece and the second logical control piece that closes is controlled the logical of gas circuit in extraction pipe and gas-filled valve and closed by operating first, fill cooperation through repeatedly taking out, finally can realize the object that forms culture environment in culture tank.
Especially when gas-filled valve is during at least two, the cultivation source of the gas that each gas-filled valve is communicated with is to be set as difference, gas-filled valve can be communicated with respectively different cultivation sources of the gas, the cooperation of filling action by taking out realizes the object that forms culture environment in culture tank, make this anaerobic culture device not only cultivate source of the gas applicable to single without oxygen gas mixture, can also be applicable to the combination of nitrogen, carbonic acid gas and hydrogen, expand the cultivation source of the gas scope of application of anaerobic culture device.
Brief description of the drawings
Fig. 1 is the profile schematic diagram of a kind of embodiment of the application's culture device for anaerobic bacteria;
Fig. 2 is the cut-away view of a kind of embodiment of the application's culture device for anaerobic bacteria;
Fig. 3 is the gas circuit connection diagram of a kind of embodiment of the application's culture device for anaerobic bacteria.
Embodiment
By reference to the accompanying drawings the present invention is described in further detail below by embodiment.
The application is defined as follows following technical name:
Anaerobism cultivate (anaerobic culture) refer to microorganism be placed in molecular oxygen state of isolation under the cultivation carried out.
Cultivate that source of the gas comprises nitrogen, carbonic acid gas, hydrogen and without oxygen gas mixture.
Be generally 80% nitrogen without oxygen gas mixture, 10% carbonic acid gas, 10% hydrogen.
Culture environment comprises anaerobic environment, micro-aerobic environment and carbon dioxide environment.
Micro-aerobic environment refers to containing oxygen (molecular state) and measures the culture environment when few.
Embodiment:
Please refer to Fig. 1 and 2, the present embodiment provides a kind of anaerobism culture systems, and it comprises anaerobic culture device and at least one culture tank 9.
This anaerobic culture device comprises housing 1, display module 2, vacuum pump 3, gas-filled valve (not shown body portion only illustrates inflation entrance 4), power module 5, logical control piece 6, multiple-pass joint 7 and the pressure transmitter 8 of closing.
This housing 1 comprises base 11 and upper cover 12, this base 11 and upper cover 12 surround one cavity are installed, housing 1 provides support for anaerobic culture device, vacuum pump 3, gas-filled valve, power module 5, logical control piece 6, multiple-pass joint 7 and the pressure transmitter 8 of closing are all arranged in installation cavity, and display module 2 is arranged at the outside surface of housing 1.
This display module 2 is to be display screen, and its data for showing that pressure transmitter 8 records, so that operator's reference.Or, display module 2 can be also external connection module, be that anaerobic culture device does not comprise display module 2, but by the external display module of socket, the data of this display module in order to show that pressure transmitter 8 records, an even all right external controller, becomes the more understandable information of operator by measured data-switching, for example simulated pressure signal intensity is changed into pressure dial plate and numeral demonstration, so that operator is easier to understand the pressure size of installing in cavity this moment.
This vacuum pump 3 is as the extraction sector in the present embodiment, and it has the extraction pipe body portion of simplified construction not shown extraction pipe (in the accompanying drawing 2 for), and this extraction pipe has intake interface, and is communicated with culture tank 9 by intake interface.This extraction pipe is provided with first and logical close the logical of gas circuit in control piece control extraction pipe and close.
In the present embodiment, three gas-filled valves are as an inflation mechanism, and inflation mechanism can be also that other can be realized and cultivate that source of the gas is communicated with and the structure of inflating in other embodiments, and the quantity of inflation mechanism can be also that one group, two groups, three groups are even more.One group of corresponding at least one culture tank 9 of giving of inflation mechanism is inflated, and that is to say in the time having many group inflation mechanisms, can inflate multiple culture tank 9 simultaneously, makes it reach culture environment.Every group of inflation mechanism can comprise at least one gas-filled valve, but gas-filled valve is while being one, this gas-filled valve need to connect without oxygen gas mixture.When gas-filled valve is during at least two, gas-filled valve is connected for the cultivation source of the gas different from least two kinds, or respectively with air and above cultivation source of the gas in one connect, the inflation outlet that gas-filled valve has and cultivates the inflation entrance of source of the gas or air communication and be communicated with culture tank 9, is respectively arranged with the second logical close that control piece carries out gas-filled valve logical simultaneously and closes control on each gas-filled valve.
In the present embodiment, first logical closes control piece and the second logical control piece that closes is referred to as the logical control piece 6 that closes, it can be machinery valve, the valve body of this machinery valve is positioned in the gas circuit of extraction pipe or gas-filled valve, in the time adopting machinery valve, this anaerobic culture device can not arrange power module (self has power module vacuum pump).This logical control piece 6 that closes also can be for electrically operated valve, as magnetic valve or pile-up valve, here taking magnetic valve as example.When logical, when closing control piece 6 and adopting electrically operated valve, anaerobic culture device also comprises power module, for example internal battery or external plug.This electrically operated valve is electrically connected with power module, and each electrically operated valve is respectively equipped with the break-make of trigger switch control electrically operated valve power source, and (trigger switch is the conventional switch of prior art, as keyswitch, twist switch etc., here not shown), be respectively used to the control of operator to extraction pipe and each gas-filled valve.
This is logical closes control piece 6 and can manually be operated by operator.Or logical while closing control piece 6 for electrically operated valve, also can make anaerobic culture device comprise controller, first logical closes control piece and the second logical trigger switch that closes control piece is connected with controller signals, is started and is closed first lead to and close control piece and second and lead to and close control piece by controller.The controller of this controller and above-mentioned display module can be same controller.
Certainly, also can comprise cultivation source of the gas in this anaerobism culture systems, this cultivation source of the gas is communicated with culture tank by corresponding gas-filled valve, inflates, to this following being described in detail.Or in other embodiments, itself does not have cultivation source of the gas anaerobism culture systems, but by its inflation entrance with outside cultivation source of the gas by pipeline communication, inflate thereby realize.
The present embodiment describes as an example of three gas-filled valves example, three gas-filled valves is called successively to the first gas-filled valve, the second gas-filled valve and the 3rd gas-filled valve here.The body portion of simplified construction not shown gas-filled valve (in the accompanying drawing 2 for) as illustrated in fig. 1 and 2, the corresponding B mouth of this first gas-filled valve, the corresponding C mouth of the second gas-filled valve, the corresponding D mouth of the 3rd gas-filled valve, here tri-inflation entrances of BCD are called to the first inflation entrance, second successively and inflate outlet and the 3rd inflation entrance, these three gas-filled valves are connected with cultivation source of the gas or air by inflation entrance separately.
As shown in Figure 3, the inflation outlet (not shown) of these three gas-filled valves and the intake interface of extraction sector are directly communicated in the shank 71 of multiple-pass joint 7, multiple-pass joint 7 is directly connected by pipeline and culture tank 9, wherein inflating entrance 4(is B, C, D mouth) and multiple-pass joint 7 be A mouth for connecting the joint 72(of culture tank 9) can expose to outside housing 1, it is dismountable making culture tank 9, cultivating between source of the gas and anaerobic culture device, make anaerobic culture device can independently pack, transport, be convenient to and the assembling of cultivating source of the gas and culture tank simultaneously.Certainly, multiple-pass joint 7, as an adapter, is communicated with by a pipeline each gas-filled valve, extraction sector after integrating again with culture tank, can simplify the structure of gas-filled valve, extraction pipe, but in other embodiments, gas-filled valve and extraction pipe are still and can be directly connected with culture tank by pipe connecting.This pressure transmitter 8 is arranged in the gas circuit of multiple-pass joint 7, is used for detecting gaseous tension in gas circuit and sends display module 2 or external controller to as control signal, now pressure transmitter 8 can be considered as to a part for anaerobic culture device.Or pressure transmitter also can be arranged in the tank body of culture tank, and gas in culture tank is measured.Certainly, in other embodiments, also can adopt independent pressure transmitter by the interior gaseous tension of culture tank 9 is measured, and coordinate outside display module to show,
Because the present embodiment adopts three road gas-filled valves, three road gas-filled valves can connect different cultivation sources of the gas, thereby realize anaerobism, micro-different culture environment of oxygen and carbon dioxide that need.Here be described in detail as follows:
Tetra-interface definitions of ABCD are as follows:
A: connect culture tank 9;
B: connect without oxygen gas mixture or hydrogen or leave a blank (leave a blank and be equivalent to connect air, be lower same);
C: connect carbon dioxide or leave a blank;
D: connect nitrogen or leave a blank.
Optional following multiple cultivation source of the gas scheme is used for realizing anaerobic environment:
1, B mouth connects without oxygen gas mixture, and D mouth and C mouth are left a blank and do not connect cultivation source of the gas, and the gas-filled valve of D mouth and C mouth correspondence is in closing condition.
Because the interior gas of culture tank 9 need to repeatedly be taken out and fills circulation and could realize anaerobic environment, as shown in Figure 3, the working process that this anaerobism is cultivated gas is as follows: after cultivation product are put into culture tank 9, culture tank 9 is connected to anaerobic culture device, open the corresponding switching of controlling extraction pipe of magnetic valve SV1(magnetic valve SV1), joint extraction pipe, power module 5 is started working from the interior extracting gases of culture tank 9, and after be evacuated down to-85kPa, magnetic valve SV1 disconnects.The then corresponding switching of controlling the gas-filled valve of connecting with anaerobic hybrid gas source of magnetic valve SV2(magnetic valve SV2) connect and be filled with without oxygen gas mixture in culture tank 9, when the anaerobic mixture pressure being filled with in culture tank 9 reaches after 0kPa, magnetic valve SV2 disconnects, and stops being filled with without oxygen gas mixture in culture tank 9.And then connect magnetic valve SV1 culture tank 9 is bled, after be evacuated down to-85kPa, shut electromagnetic valve SV1 again, opens magnetic valve SV2 and inflates, and so repeatedly takes out the anaerobic culture environment of filling the interior generation 100% of culture tank 9 after step for three times.
2, B mouth connects without oxygen gas mixture, and D mouth connects nitrogen, and C mouth is left a blank and do not connect cultivation source of the gas, and the gas-filled valve of C mouth correspondence is in closing condition.
Because repeatedly take out the gas being filled with for the last time in the process of filling and be only the gas of effective use, therefore the low cultivation gas of the inflatable body process of taking out available rates above replaces.
The working process that this anaerobism is cultivated gas is as follows: after cultivation product are put into culture tank 9, culture tank 9 is connected to anaerobic culture device, open the corresponding switching of controlling extraction pipe of magnetic valve SV1(magnetic valve SV1), connect extraction pipe from the interior extracting gases of culture tank 9, after be evacuated down to-85kPa, magnetic valve SV1 disconnects.The then corresponding switching of controlling the gas-filled valve of connecting with nitrogen of magnetic valve SV4(magnetic valve SV4) connect be filled with nitrogen in culture tank 9, when the nitrogen pressure being filled with in culture tank 9 reaches after 0kPa, magnetic valve SV4 disconnects, and stops being filled with nitrogen in culture tank 9.And then connect magnetic valve SV1 culture tank 9 is bled, after be evacuated down to-85kPa, shut electromagnetic valve SV1 again, open magnetic valve SV4 and carry out inflated with nitrogen, when the nitrogen pressure being filled with in culture tank 9 reaches after 0kPa, magnetic valve SV4 disconnects, and stops being filled with nitrogen in culture tank 9.Finally, open magnetic valve SV1 again to after be evacuated down in culture tank 9-85kPa, disconnect magnetic valve SV1, then the corresponding switching of controlling the gas-filled valve of connecting with anaerobic hybrid gas source of magnetic valve SV2(magnetic valve SV2) connect, to being filled with in culture tank 9 without oxygen gas mixture to 0kPa, make the anaerobic culture environment of the interior generation 100% of culture tank 9.
In this programme, if do not considered gas cost, gas that also can be using hydrogen or carbonic acid gas as front twice inflation, replaces being filled with of nitrogen with hydrogen or carbonic acid gas, is still and is filled with without oxygen gas mixture for the last time.
3, D mouth connects nitrogen, and C mouth connects carbon dioxide, and B mouth connects hydrogen, and three kinds of gas content ratios in the mixed gas of generation are just adjustable.
The difference part of this programme and scheme two is, in the time of front twice inflation, can be filled with any gas of nitrogen, carbonic acid gas and hydrogen, as being first filled with the nitrogen that price is lower, and open the corresponding switching of controlling the gas-filled valve of connecting with hydrogen of magnetic valve SV2(magnetic valve SV2 while inflating the last time), the corresponding switching of controlling the gas-filled valve of connecting with carbonic acid gas of SV3(magnetic valve SV3) and the switching of the gas-filled valve of the corresponding control of SV4(magnetic valve SV4 and nitrogen connection) be filled with in proportion three kinds of gases, can form preset proportion without oxygen gas mixture.
Realize micro-aerobic culture environment scheme as follows:
As formed micro-aerobic environment in culture tank 9, still can realize by above-mentioned three kinds of schemes, its solenoid control is all consistent.But according to the difference of oxygen level, take out the number of times difference of filling circulation, if oxygen level is between 1% to 4%, need to experience secondary and take out inflatable body circulation, after power module 5 is taken the gas of culture tank 9 for the second time away, be filled with without oxygen gas mixture, or be filled with nitrogen respectively, carbonic acid gas and hydrogen forms without oxygen gas mixture.And oxygen level is between 4% to 6% time, only need experience once to take out inflatable body circulation, take away for the first time after the gas of culture tank 9 at power module 5, be filled with without oxygen gas mixture, or be filled with respectively nitrogen, carbonic acid gas and hydrogen and form without oxygen gas mixture, thereby form micro-aerobic environment.
The scheme that realizes carbonic acid gas culture environment is as follows:
C mouth connects carbon dioxide, and B mouth and D mouth are left a blank.
After cultivation product are put into culture tank 9, culture tank 9 is connected to anaerobic culture device, open the corresponding switching of controlling extraction pipe of magnetic valve SV1(magnetic valve SV1), joint extraction pipe, power module 5 is started working from the interior extracting gases of culture tank 9, after be evacuated down to-85kPa, magnetic valve SV1 disconnects power module 5 simultaneously and quits work.The then corresponding switching of controlling the gas-filled valve of connecting with carbonic acid gas of magnetic valve SV3(magnetic valve SV3) connect be filled with a certain proportion of carbonic acid gas in culture tank 9, then magnetic valve SV2 or magnetic valve SV3 open, and be filled with air and form required CO after 0kPa
2culture environment.
In like manner, according to the above-mentioned scheme that realizes carbonic acid gas culture environment, also can realize nitrogen culture environment and hydrogen culture environment.
The anaerobism culture systems that the present embodiment provides can realize anaerobism, micro-oxygen and carbon dioxide culture environment that needs under above multiple different cultivation gas source condition, and the scope of application is wider, and operation is also enough simple and convenient simultaneously.
Three gas-filled valves shown in the present embodiment are only a kind of examples, also can adopt in other embodiments a gas-filled valve, as adopt a gas-filled valve to be equivalent to C, D mouth in such scheme 1 to remove.As adopt two gas-filled valves, except the scheme 2 of cultivating for anaerobic environment above, other schemes are still all applicable.Or also can adopt more gas-filled valves, in the time of more gas-filled valves, also all can realize anaerobism, micro-oxygen and carbon dioxide culture environment that needs according to the control step of above each scheme.
Further, the present embodiment can be realized anaerobism, micro-aerobic and and CO by manual operation
2culture environment, and in other embodiments, also can be at controller built-in computer software program, thus realize the automatic control of each parts such as magnetic valve by controller, control action can be with reference to described in above each scheme, and operator only need input the parameter or the preference pattern that need.
Above content is in conjunction with concrete embodiment further description made for the present invention, can not assert that specific embodiment of the invention is confined to these explanations.For general technical staff of the technical field of the invention, without departing from the inventive concept of the premise, can also make some simple deduction or replace.
Claims (10)
1. an anaerobic culture device, is characterized in that, comprising:
Housing, described housing is that anaerobic culture device provides support;
Extraction sector, described extraction sector is arranged in housing, and it has extraction pipe, and described extraction pipe has the intake interface being communicated with culture tank, and described extraction pipe is provided with first and logical close the logical of gas circuit in control piece control extraction pipe and close;
Also comprise at least one group of inflation mechanism, one group of corresponding at least one culture tank of giving of inflation mechanism is inflated; Described in every group, inflation mechanism comprises at least one gas-filled valve, the inflation outlet that each gas-filled valve has and cultivates the inflation entrance of source of the gas or air communication and is communicated with culture tank, and on each gas-filled valve, be respectively arranged with the second logical close that control piece carries out the interior gas circuit of gas-filled valve logical and close control.
2. anaerobic culture device as claimed in claim 1, is characterized in that, described first logical closes control piece and/or second logical to close control piece be machinery valve, and the valve body of described machinery valve is positioned in the gas circuit of extraction pipe or gas-filled valve.
3. anaerobic culture device as claimed in claim 1, it is characterized in that, described anaerobic culture device also comprises power module, described first logical closes control piece and/or second logical to close control piece be electrically operated valve, described electrically operated valve is electrically connected with power module, and each electrically operated valve is respectively equipped with the break-make of trigger switch control electrically operated valve power source.
4. anaerobic culture device as claimed in claim 3, it is characterized in that, described anaerobic culture device also comprises controller, described first logical closes control piece and the second logical trigger switch that closes control piece is connected with controller signals, is started and is closed first lead to and close control piece and second and lead to and close control piece by described controller.
5. the anaerobic culture device as described in any one in claim 1 to 4, it is characterized in that, described in every group, inflation mechanism comprises that at least two for being connected to few two kinds of different gas-filled valves of cultivating source of the gas, described anaerobic culture device also comprises multiple-pass joint, the intake interface of described extraction pipe and the inflation of gas-filled valve outlet are all directly communicated in multiple-pass joint, and described multiple-pass joint direct and culture tank is connected.
6. anaerobic culture device as claimed in claim 5, is characterized in that, described anaerobic culture device also comprises pressure transmitter and display module, and described pressure transmitter is arranged at multiple-pass joint for connecting the gas circuit of culture tank.
7. an anaerobism culture systems, is characterized in that, comprises anaerobic culture device and at least one culture tank as described in claim 1-6 any one; Extraction pipe in described anaerobic culture device is communicated in the tank body of described culture tank, and logical close the logical of gas circuit in control piece control extraction pipe and close by first, gas-filled valve in described inflation mechanism is corresponding to be communicated with the tank body of at least one culture tank, and leads to and close the logical of gas circuit in control piece control gas-filled valve and close by second.
8. anaerobism culture systems as claimed in claim 7, it is characterized in that, described anaerobism culture systems also comprises cultivation source of the gas, described cultivation source of the gas comprises without oxygen gas mixture, and any in carbon dioxide, nitrogen and three kinds of gases of hydrogen, described inflation mechanism comprises two gas-filled valves, the inflation entrance of one of them gas-filled valve with without oxygen gas mixture connect, any connection in the inflation entrance of another gas-filled valve and carbon dioxide, nitrogen and three kinds of gases of hydrogen.
9. anaerobism culture systems as claimed in claim 7, it is characterized in that, described anaerobism culture systems also comprises cultivation source of the gas, described cultivation source of the gas comprises carbon dioxide, nitrogen and three kinds of cultivation sources of the gas of hydrogen, described inflation mechanism comprises three gas-filled valves, the inflation entrance of first gas-filled valve and carbon dioxide are connected, and the inflation entrance of second gas-filled valve and nitrogen are connected, and the inflation entrance of the 3rd gas-filled valve and hydrogen are connected.
10. anaerobism culture systems as claimed in claim 7, it is characterized in that, described anaerobism culture systems also comprises cultivation source of the gas, described cultivation source of the gas comprises carbon dioxide, described inflation mechanism comprises two gas-filled valves, the inflation entrance of one of them gas-filled valve is communicated with carbonic acid gas, and the inflation entrance of another gas-filled valve is left a blank.
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|---|---|---|---|
| CN201420187436.1U CN203794901U (en) | 2014-04-17 | 2014-04-17 | Anaerobic culturing device and system |
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| CN201420187436.1U CN203794901U (en) | 2014-04-17 | 2014-04-17 | Anaerobic culturing device and system |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106148178A (en) * | 2015-04-02 | 2016-11-23 | 复旦大学附属华山医院 | The culture systems of a kind of static killing curve for anti-anaerobic agent and construction method thereof |
| CN107586703A (en) * | 2017-11-09 | 2018-01-16 | 安徽普生源生物科技有限公司 | Microbial cultivation device |
| CN113832017A (en) * | 2021-06-21 | 2021-12-24 | 内蒙古工业大学 | Intelligent parallel anaerobic culture fermentation system |
-
2014
- 2014-04-17 CN CN201420187436.1U patent/CN203794901U/en not_active Expired - Lifetime
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106148178A (en) * | 2015-04-02 | 2016-11-23 | 复旦大学附属华山医院 | The culture systems of a kind of static killing curve for anti-anaerobic agent and construction method thereof |
| CN107586703A (en) * | 2017-11-09 | 2018-01-16 | 安徽普生源生物科技有限公司 | Microbial cultivation device |
| CN113832017A (en) * | 2021-06-21 | 2021-12-24 | 内蒙古工业大学 | Intelligent parallel anaerobic culture fermentation system |
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