CN113832017A

CN113832017A - Intelligent parallel anaerobic culture fermentation system

Info

Publication number: CN113832017A
Application number: CN202110684513.9A
Authority: CN
Inventors: 刘占英; 齐咏生; 刘洋; 杜晓旭
Original assignee: Inner Mongolia University of Technology
Current assignee: Inner Mongolia University of Technology
Priority date: 2021-06-21
Filing date: 2021-06-21
Publication date: 2021-12-24

Abstract

The invention discloses an intelligent parallel anaerobic culture fermentation system which comprises a fermentation tank body (8), a nutrient liquid dripping device, an anaerobic environment control execution device and an air pressure monitoring module, wherein the air pressure monitoring module is arranged on a visual operation platform. The design of the four-pipe-hole fermentation tank body is respectively responsible for the injection of culture solution, the injection of nitrogen, the extraction of air and the monitoring of pressure, so that the time of nitrogen injection and gas extraction is more accurate, a higher-precision anaerobic environment is manufactured, and a signal is transmitted to an operation table by a pressure module and is visually displayed by an LCD (liquid crystal display), and the intelligent control of anaerobic bacteria fermentation is realized. The automatic control system designed by the invention optimizes the production process and forms a functional automatic control system for the anaerobic microorganism fermentation process. The purpose of this system is to solve anaerobic bacteria fermentation process anaerobism condition and difficult manufacturing and difficult control problem.

Description

Intelligent parallel anaerobic culture fermentation system

Technical Field

The system can be used for various anaerobic bacteria culture fermentation projects, realizes automatic production of culture and fermentation of various anaerobic bacteria, and belongs to the technical field of fermentation.

Background

At present, the fermentation industry in China is in the key stage of upgrading transformation, and how to accurately control the fermentation reaction process, improve the reaction efficiency and reduce the resource consumption is the future development trend. In recent years, with the rapid development of microbial fermentation, biosensing technology and related products, the research and development of universal and miniaturized biological fermentation equipment at home and abroad become more and more important. However, the conventional manual operation control system is usually adopted in the small-sized microbial fermentation device in the laboratory at present, and the high-precision control requirement of the current multi-variety microbial fermentation is difficult to meet. Meanwhile, since the cultivation of anaerobic microorganisms in a laboratory requires severe anaerobic conditions, a corresponding small-sized apparatus is lacking.

Disclosure of Invention

The invention aims to design a four-pipe-hole fermentation tank body respectively responsible for culture solution injection, nitrogen injection, air extraction and pressure monitoring on the basis of the current domestic and foreign existing fermentation equipment and fermentation technology research, so that the nitrogen injection and gas extraction time is more accurate, a higher-precision anaerobic environment is manufactured, signals are transmitted to an operation table by a pressure module and are visually displayed by an LCD (liquid crystal display), and the intelligent control of anaerobic bacteria fermentation is realized. The automatic control system designed by the invention optimizes the production process and forms a functional automatic control system for the anaerobic microorganism fermentation process. The purpose of this system is to solve anaerobic bacteria fermentation process anaerobism condition and difficult manufacturing and difficult control problem.

In order to achieve the purpose, the system provides an intelligent parallel anaerobic culture fermentation system which is used in an anaerobic bacteria fermentation process.

The invention adopts the technical scheme that the intelligent parallel anaerobic culture fermentation system comprises a fermentation tank body 8, a nutrient liquid dripping device, an anaerobic environment control execution device and an air pressure monitoring module, and is arranged on a visual operation platform.

In the anaerobic environment control execution device, a button switch 1 controls a CPU core module 2, and output data is displayed through an LED display module 5.

The nutrient liquid dripping device comprises a nitrogen electric valve 11, an air control valve 12, a pressure sensor 13 and a culture liquid dripping control valve 14, and the nitrogen electric valve 11, the air control valve 12, the pressure sensor 13 and the culture liquid dripping control valve 14 are fixed in the heat dissipation shell 3 below the operating platform.

Furthermore, the nutrient solution dripping device also comprises an electric dripping valve 16, a telescopic dripping rubber tube 17, a rotary wheel disc driving device 18, a rotary wheel disc 19 and a movable bracket 20. The electric dropping valve 16 is connected with the telescopic dropping rubber tube 17 by screw thread and fixed on the bayonet position of the rotary wheel disc 19. The rotary wheel disc driving device 18 is connected with the rotary wheel disc 19 through a movable bracket 20 and is arranged in the sliding groove.

Furthermore, the anaerobic environment control execution device controls the nitrogen electric valve 11 to open through the CPU core module 2 to fill nitrogen into the fermentation tank, the pressure monitoring module transmits the pressure in the tank body into the CPU core module until the pressure reaches a set pressure value, then the nitrogen electric valve 11 is controlled to close, and simultaneously the vacuum pump 4 and the four air control valves 12 are opened to pump out the gas in the tank body for 5 seconds, so that the circulation is carried out, and the anaerobic environment in the fermentation tank is guaranteed.

Further, a control panel device consisting of the button switch 1 and the LED display module 5 is embedded above the whole workbench and forms an angle of 60 degrees with the horizontal plane, so that daily operation and observation are facilitated.

Further, six culture solution conveying rubber pipes 6 which are connected in series and arranged on the upper half part of the workbench side by side can be embedded on the rotary wheel disc 7, or the culture solution conveying rubber pipes 6 can be used independently to form a culture solution conveying unit of the system; the two groups of culture solution conveying units are arranged in parallel and are controlled by the CPU core module 2 together, and the types, frequency and flow of the added nutrient solution are intelligently selected.

Furthermore, the fermentation tank bodies 8 have three groups, each group of fermentation tank bodies 8 is provided with a pipe hole for adding a culture solution, and thalli and the culture solution are injected through the pipe hole before the device is started; a culture solution pipe hole for injecting nitrogen is controlled by the CPU core module 2 to control the nitrogen electric valve to inject nitrogen into the culture solution pipe hole; a culture solution pipe hole for pumping gas in the tank, wherein the gas in the tank is pumped out from the hole by controlling a vacuum pump 4 and an air control valve 12 through a CPU core module 2; a hole for a culture solution pipe for monitoring the pressure in the tank is provided, and a pressure sensor 13 is installed therein for monitoring the pressure in the fermenter. Three groups of parallel fermentation tank bodies 8 are fixed in a groove of the heat dissipation shell 3 below the workbench, and three groups of one-to-one parallel reference experiments are carried out simultaneously.

Furthermore, the nitrogen electric valve 11 and the air control valve 12 are arranged on the fermentation tank body 8 in a flange connection mode, the flange connection mode is convenient to install and disassemble, and the nitrogen electric valve and the air control valve are not easy to leak and have high safety factors under the condition that high temperature and high pressure are generated in the fermentation tank body 8 through fermentation reaction.

Further, the pressure sensor 13 is connected by screw threads, so that the sealing effect is not influenced. The culture solution dripping tank opening 15 is welded on the fermentation tank body 8, and the culture solution dripping control valve 14 is added with the intelligent infrared device and is in butt joint with the culture solution conveying rubber tube 6, so that the intelligent addition of the nutrient solution in the whole process is realized.

Further, the pressure monitoring module collects the pressure condition in the fermentation tank body 8 through the pressure sensor 13 and outputs the pressure condition to the operation table through the LCD display module 5, the pressure in the fermentation tank body is monitored in real time in the operation table, and the fermentation environment in the fermentation tank body 8 is controlled by combining with the anaerobic environment control execution device.

Compared with the prior art, the fermentation system designed by the invention is a laboratory microorganism culture system for microorganism or cell culture, but the fermentation system in the prior art has single function and is not intelligent, and can not effectively give consideration to the tightness, automatic addition and anaerobic environment control, and nutrient substances can not be added at any time when anaerobic culture is required. Compared with the prior art, the invention adopts the design of the four-pipe-hole fermentation tank body which is respectively responsible for the injection of culture solution, the injection of nitrogen, the extraction of air and the monitoring of pressure, so that the time for the injection of nitrogen and the extraction of air is more accurate, a higher-precision anaerobic environment can be manufactured on the premise of ensuring the addition at any time, and the pressure module transmits signals to the operation table to be visually displayed by the LCD, thereby realizing the intelligent control of anaerobic bacteria fermentation.

Drawings

Fig. 1 is a schematic view of the overall appearance structure of the present invention.

Fig. 2 is a schematic view of the internal structure of the present invention.

FIG. 3 is a schematic structural diagram of the intelligent parallel anaerobic culture fermentation system.

1. A push button switch; 2. a CPU core module; 3. a heat dissipating housing; 4. a vacuum pump; 5. an LCD display module; 6. a culture solution conveying rubber tube; 7 rotating the wheel disc; 8. a fermentation tank body; 9. a nitrogen tank; 10. a bin gate.

FIG. 4 is a schematic view of a fermentation tank of the intelligent parallel anaerobic culture fermentation system provided by the invention.

11. A nitrogen electric valve; 12. an air control valve; 13. a pressure sensor; 14. a culture solution dripping control valve; 15. the culture liquid is dripped into the tank mouth.

FIG. 5 is a schematic view of the intelligent parallel anaerobic culture fermentation system culture liquid adding device.

16. An electric dropping valve; 17. a flexible dropping rubber tube; 18. a rotary wheel disc drive device; 19. rotating the wheel disc; 20. and moving the support.

Detailed Description

The present invention will be described in detail below with reference to the accompanying drawings and examples.

The utility model provides a parallel anaerobic culture fermentation system of intelligence, this system is a culture system suitable for anaerobic microorganism, can solve the difficult problem that special fermentation cylinder of present anaerobic microorganism and culture system lack, especially there is not the parallel anaerobic culture fermentation system of intelligence to cultivate the poor problem of accuracy that the fermentation system caused for anaerobic microorganism cultivates yet.

The system comprises four parts: the fermentation tank comprises a fermentation tank body, a nutrient liquid dripping device, an anaerobic environment control execution device and an air pressure monitoring module.

The fermentation tank body is provided with three fermentation tank bodies, each fermentation tank body is provided with a pipe hole for dripping culture solution, and thalli are injected through the pipe holes; a pipe hole for injecting nitrogen or carbon dioxide; a tube hole for extracting gas in the tank; and a tube hole for monitoring the pressure in the tank body, wherein the tube hole for feeding the feed material and the tube hole for inoculating the strain can share one tube hole.

The nutrient solution dripping device mainly comprises three culture solution dripping control valves, two dripping wheel discs controlled by stepping motors and twelve culture solution conveying pipes. The anaerobic environment control execution device mainly comprises a nitrogen injection module, an air extraction module, a CPU core processing module and a shell with a heat dissipation groove. The air pressure monitoring module consists of three pressure sensors and an LCD display module. In the anaerobic environment control execution device, the nitrogen injection module is formed by connecting three air valve control fermentation tank ports controlled by a CPU (central processing unit) with a nitrogen tank, and the CPU controls the injection time of nitrogen. In the culture solution dropwise add device, the culture solution conveyer pipe is fixed in the operation panel, can select the rubber tube to connect and fix to rotatory rim plate in according to culture solution kind quantity when needing, and dropwise add rim plate accessible step motor control is rotatory to drip different feed supplements to the fermentation cylinder. The air extraction module is a vacuum pump controlled by a CPU to start and stop and is connected with the three fermentation tanks, and the CPU controls the air extraction time. The CPU core processing module comprises a PLC (programmable logic controller) and an EM235 analog input module connected with the PLC, the PLC is also connected with the nitrogen electric valve and the vacuum pump, and the EM235 analog input module is connected with the pressure sensor. The system solves the problem that anaerobic conditions are difficult to create and maintain during anaerobic bacteria fermentation, so that the anaerobic bacteria are easier to culture and used for fermentation. The structure of the system is shown in the following figure 1, and the internal structure is shown in figure 2.

An intelligent parallel anaerobic culture fermentation system is used for the fermentation of anaerobe, and the device comprises: and pressing a button, starting the system, opening a culture solution injection valve, controlling the rotary wheel disc to rotate by the CPU control module to inject different culture solutions, and injecting the culture solution into the next fermentation tank body by moving left and right until all the culture solutions are injected, and closing the tank body valve. The pressure sensor inputs analog quantity into the CPU control module through the EM235 analog quantity input module, the CPU outputs the pressure of each fermentation tank body through the LCD display module, the CPU control module fills nitrogen into the tank body through controlling the electric nitrogen valve, when the pressure reaches a set value, the electric nitrogen valve is controlled to be closed, meanwhile, the air control valve is opened, then air in the tank body is pumped out for 5 seconds through controlling the vacuum pump, and the circulation is carried out, so that the manufacturing and the maintaining of the anaerobic environment in the tank body are realized.

The button is pressed, the system is started, the culture solution injection valve is opened, the CPU control module controls the rotary wheel disc driving device to drive the rotary wheel disc to inject different culture solutions, the culture solution is injected into the next fermentation tank body through left and right movement, until all the culture solutions are injected, the tank body valve is closed, and the tank body is rotated to the initial position. The whole culture solution injection process is completed by the system, and the aseptic operation is ensured to the maximum extent.

Each fermentation tank body is provided with a pipe hole for dropwise adding supplementary materials and supplementing sugar or ammonia, and culture solution and thalli are injected through the pipe hole; a pipe hole for injecting nitrogen or carbon dioxide; a tube hole for extracting gas in the tank; an orifice for monitoring the pressure in the tank.

The pressure sensor inputs analog quantity into the CPU control module through the EM235 analog quantity input module, the CPU outputs the pressure of each fermentation tank body through the LCD display module, the PLC controller charges nitrogen into the tank body through controlling the electric nitrogen valve, when the pressure reaches a set value, the electric nitrogen valve is controlled to be closed, the air control valve is opened at the same time, then air in the tank body is pumped out for 5 seconds through controlling the vacuum pump, and the circulation is carried out, so that the oxygen content in the tank body is reduced to the maximum extent, and an anaerobic environment is provided for microbial fermentation in the fermentation tank.

As shown in figure 3, the fermentation tank body of the intelligent parallel anaerobic culture fermentation system is arranged in the front of a heat dissipation shell under an operation platform, a nitrogen tank is arranged in the rear, a vacuum pump is arranged on the other side, and an air control valve and a pressure sensor are respectively arranged at the corresponding tank hole of each tank body.

As shown in figure 4, in the air pressure monitoring module of the system, a pressure sensor is installed on a hole of a fermentation tank, the system starts to operate through a button switch, the pressure sensor can acquire an analog signal output by the pressure in the tank body, and the analog signal is transmitted to a CPU module through an EM235 analog input module to be processed and then displayed on an operation table through an LCD display module.

Install CPU control module on the operation panel, through button switch, the system begins to operate, each module of system accomplishes the initialization, culture solution injection valve is opened, by the rotatory different culture solutions of injection of CPU control module control rotary wheel dish, remove to control to pour into the culture solution to next fermenter body about the rethread, it finishes until all culture solution injection, close a jar body valve, PLC control module control nitrogen gas electric valve opens and fills nitrogen gas into to the fermenter, until reaching the set pressure value, then PLC controller control nitrogen gas electric valve closes and opens vacuum pump and four air control valves simultaneously and take out gaseous 5 seconds in the tank body, so circulate, the thermovent can get rid of the heat in order to guarantee the normal operating of whole device.

Claims

1. The utility model provides a parallel anaerobic culture fermentation system of intelligence which characterized in that: comprises a fermentation tank body (8), a nutrient liquid dripping device, an anaerobic environment control execution device and a gas pressure monitoring module;

in the anaerobic environment control execution device, a button switch (1) controls a CPU core module (2) and displays output data through an LED display module (5); the nutrient solution dripping device comprises a nitrogen electric valve (11), an air control valve (12), a pressure sensor (13) and a culture solution dripping control valve (14), and the nitrogen electric valve (11), the air control valve (12), the pressure sensor (13) and the culture solution dripping control valve (14) are fixed in a heat dissipation shell (3) below the operating platform; the pressure monitoring module collects the pressure condition in the fermentation tank body (8) through the pressure sensor (13), outputs the pressure condition to the operation table through the LCD display module (5), monitors the pressure in the tank body in real time in the operation table, and controls the fermentation environment in the fermentation tank body (8) by combining with the anaerobic environment control execution device.

2. The intelligent parallel anaerobic culture fermentation system of claim 1, wherein: the nutrient solution dripping device also comprises an electric dripping valve (16), a telescopic dripping rubber tube (17), a rotary wheel disc driving device (18), a rotary wheel disc (19) and a movable bracket (20); the electric dropping valve (16) is connected with the telescopic dropping rubber tube (17) by screw threads and is fixed on the bayonet position of the rotary wheel disc (19); the rotary wheel disc driving device (18) is connected with the rotary wheel disc (19) through a movable bracket (20) and is arranged in the sliding groove.

3. The intelligent parallel anaerobic culture fermentation system of claim 1, wherein: the anaerobic environment control execution device controls the nitrogen electric valve (11) to open through the CPU core module (2) to fill nitrogen into the fermentation tank, the pressure monitoring module transmits the pressure in the tank body into the CPU core module until a set pressure value is reached, and then the nitrogen electric valve (11) is controlled to close and simultaneously the vacuum pump (4) and the four air control valves (12) are opened to pump out the gas in the tank body.

4. The intelligent parallel anaerobic culture fermentation system of claim 1, wherein: the control panel device composed of the button switch (1) and the LED display module (5) is embedded above the whole workbench and forms an angle of 60 degrees with the horizontal plane.

5. The intelligent parallel anaerobic culture fermentation system of claim 1, wherein: six culture solution conveying rubber pipes (6) which are connected in series and arranged on the upper half part of the workbench side by side can be embedded on the rotary wheel disc (7), or the culture solution conveying rubber pipes (6) can be used independently to form a culture solution conveying unit of the system; the two groups of culture solution conveying units are arranged in parallel and are controlled by a CPU core module (2) together, and the types, frequency and flow of the added nutrient solution are intelligently selected.

6. The intelligent parallel anaerobic culture fermentation system of claim 1, wherein: the fermentation tank bodies (8) are divided into three groups, each group of fermentation tank bodies (8) is provided with a pipe hole for adding a culture solution, and thalli and the culture solution are injected through the pipe hole of the culture solution; a culture solution pipe hole for injecting nitrogen is controlled by the CPU core module (2) to control the nitrogen electric valve to inject nitrogen into the culture solution pipe hole; a culture solution pipe hole for pumping gas in the tank, wherein the gas in the tank is pumped out from the hole by controlling a vacuum pump (4) and an air control valve (12) through a CPU core module (2); a hole for a culture solution for monitoring the pressure in the tank body, wherein a pressure sensor (13) is installed therein for monitoring the pressure in the fermentation tank body; three groups of parallel fermentation tank bodies (8) are fixed in a groove of the heat dissipation shell (3) below the workbench, and three groups of one-to-one parallel reference experiments are carried out simultaneously.

7. The intelligent parallel anaerobic culture fermentation system of claim 1, wherein: the nitrogen electric valve (11) and the air control valve (12) are arranged on the fermentation tank body (8) in a flange connection mode, and are convenient to install and detach in the flange connection mode.

8. The intelligent parallel anaerobic culture fermentation system of claim 5, wherein: the pressure sensor (13) is in threaded connection, so that the sealing effect is not influenced; the culture solution dripping tank opening (15) is welded on the fermentation tank body (8), and the culture solution dripping control valve (14) is added with an intelligent infrared device and is in butt joint with the culture solution conveying rubber tube (6), so that the whole-process intelligent addition of the nutrient solution is realized.