CN201762333U - Open type cell culture box - Google Patents
Open type cell culture box Download PDFInfo
- Publication number
- CN201762333U CN201762333U CN2010205196133U CN201020519613U CN201762333U CN 201762333 U CN201762333 U CN 201762333U CN 2010205196133 U CN2010205196133 U CN 2010205196133U CN 201020519613 U CN201020519613 U CN 201020519613U CN 201762333 U CN201762333 U CN 201762333U
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- CN
- China
- Prior art keywords
- cell culture
- sealing gasket
- combined sealing
- box body
- cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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Abstract
A disposable cell culture box, which has an open type structure and cell culture liquid of which is able to perform gas exchange with outside, is characterized by including a hollow cover, a screw, a box body, a combined sealing gasket, a mesh and a cell culture sheet; the cell culture sheet is installed on the bottom of the box body; the combined sealing gasket is pressed on the cell culture; the mesh is embedded on the combined sealing gasket; and the hollow cover is equipped on the upper portion of the combined sealing gasket and is fixed on the box body by the screw. Cells are cultured on the cell culture sheet; the cell culture liquid is fed into the box from one end by an injector; a gas exchange layer on the top of the combined sealing gasket performs gas exchange between the culture liquid and outside, thus guaranteeing pH balance and sufficient dissolved oxygen in the cell liquid; and the mesh controls bubbles in the culture liquid to move on the upper portion of the combined sealing gasket so as not to influence growth of cells on the cell culture sheet on the bottom of the combined sealing gasket.
Description
Technical field the utility model relates to a kind of cell culture case, particularly a kind of disposable use cell culture case with Open architecture.
Background technology at present in cytobiology field and life science the Tissue Culture Flask of widespread use be the glassiness or the plastics matter culturing bottle of standard, the function of this class Tissue Culture Flask is exactly the container that is equivalent to cell culture fluid, keeps the cell normal growth.The limitation of this class simple cell culturing bottle is fairly obvious: owing to be sealed structure, gas exchange capacity is poor, and pH balance that the cell normal growth needs in cell culture fluid and dissolved oxygen can not get in time replenishing, and will influence cell proper splitting and propagation.Simultaneously, this class Tissue Culture Flask repeatedly washes down pattern meeting influence experiment next time because degree of cleaning are not enough of use, and repeatedly washing down the scratch that causes also can influence cell image observation and sampling.
Above-mentioned deficiency has restricted experiment use and the operation of normal cell culture experiment and correlated results analysis of this class Tissue Culture Flask, therefore, develop a kind of Open architecture that has, cell culture fluid can to carry out the disposable use cell culture case of gaseous interchange very necessary with the external world.
The summary of the invention the purpose of this utility model provides a kind of disposable use, has the cell culture case that Open architecture, cell culture fluid can carry out gaseous interchange with the external world.
Open cell culture case is made up of hollow out lid, screw, box body, combination sealing pad, aperture plate and cell cultures sheet.
The cell cultures sheet is installed on box body bottom, and combination sealing pad gland is on cell cultures, and the aperture plate edge is stuck on the combination sealing pad, the hollow out lid be installed on combination sealing pad top and by screw retention on box body.
Cell cultures on the cell cultures sheet, cell culture fluid by syringe in box body one end adds box.The gas exchange layer at combination sealing top realizes nutrient solution and extraneous gaseous interchange, guarantees balance and the dissolved oxygen abundance of pH in the enchylema.Aperture plate can be controlled the nutrient solution bubble makes it can not influence cell growth on the cell cultures sheet that is positioned at the rebasing portion of combination sealing in the bubble activity of combination sealing pad top.
After the utility model adopts technique scheme, have following distinguishing feature:
1, cellular regions is Open architecture, and the gas exchange layer at combination sealing pad top can guarantee that cell culture fluid can freely carry out gaseous interchange.
2, cell culture case is designed to disposable use, need not to clean, and can not impact next cell cultures.
Description of drawings Fig. 1 shows the structure of open cell culture case
During experiment, in cell is annotated into cell cultures with syringe from the edge of combination sealing pad 4.After experiment finishes, unclamp set screw 2, remove hollow out lid 1, aperture plate 5 and combination sealing pad 4 successively after, just can take cell cultures sheet 6, box body 3 abandons.
Claims (1)
1. an open cell culture case is made up of hollow out lid, screw, box body, combination sealing pad, aperture plate and cell cultures sheet; It is characterized in that the cell cultures sheet is installed on box body bottom, combination sealing pad gland is on cell cultures, and the aperture plate edge is stuck on the combination sealing pad, the hollow out lid be installed on combination sealing pad top and by screw retention on box body.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010205196133U CN201762333U (en) | 2010-09-07 | 2010-09-07 | Open type cell culture box |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010205196133U CN201762333U (en) | 2010-09-07 | 2010-09-07 | Open type cell culture box |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201762333U true CN201762333U (en) | 2011-03-16 |
Family
ID=43714943
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010205196133U Expired - Fee Related CN201762333U (en) | 2010-09-07 | 2010-09-07 | Open type cell culture box |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN201762333U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112812966A (en) * | 2021-02-02 | 2021-05-18 | 宁波大学医学院附属医院 | Cell culture device |
-
2010
- 2010-09-07 CN CN2010205196133U patent/CN201762333U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112812966A (en) * | 2021-02-02 | 2021-05-18 | 宁波大学医学院附属医院 | Cell culture device |
| CN112812966B (en) * | 2021-02-02 | 2023-06-20 | 宁波大学医学院附属医院 | Cell culture device |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20110316 Termination date: 20130907 |