CN1989240B

CN1989240B - 含有减毒瘟病毒的疫苗

Info

Publication number: CN1989240B
Application number: CN200580024345XA
Authority: CN
Inventors: 格雷戈尔·迈耶斯; 安德烈亚斯·埃格; 克里斯琴·费策尔; 马蒂纳·冯弗赖伯格
Original assignee: Boehringer Ingelheim Vetmedica GmbH
Current assignee: Boehringer Ingelheim Vetmedica GmbH
Priority date: 2004-05-19
Filing date: 2005-05-18
Publication date: 2013-07-31
Anticipated expiration: 2025-05-18
Also published as: ES2321210T9; LU92747I2; BRPI0510396A; KR100908798B1; NZ552088A; KR20070012862A; NL300743I1; JP5006782B2; BRPI0510396B1; AR104448A2; AR049176A1; HUS1500033I1; RS50753B9; CN1989240A; MXPA06013298A; EP1751276B9; CY2015023I1; SI1751276T1; RS50753B; CY2015021I2

Abstract

本发明涉及减毒的瘟病毒，特别是减毒的BVDV，其中至少一个突变是在糖蛋白E^rns的编码序列，并且至少另一个突变在N^pro的编码序列，其优选导致除了失活(推测的)属于N^pro的免疫调节活性外，联合灭活属于糖蛋白E^rns的RNA酶活性。发明也涉及用于减弱瘟病毒例如BVDV的方法，编码该瘟病毒特别是BVDV的核酸，包括发明的减毒瘟病毒特别是BVDV的组合物和疫苗。

Description

含有减毒瘟病毒的疫苗

发明背景

技术领域

本发明涉及动物健康的领域，特别是减毒的瘟病毒如牛病毒性腹泻病毒(bovine viral diarrhea virus，BVDV)。

背景资料

瘟病毒在世界上许多国家是具有重大经济影响的动物疾病的起因。现已知的病毒分离物分属四个不同物种，它们一起形成黄病毒科的一个属。

I/II牛病毒性腹泻病毒(BVDV)1型(BVDV-1)和2型(BVDV-2)引起牛的病毒性腹泻(BVD)和家畜(cattle)的粘膜疾病(MD)(Baker，1987；Moennigand Plagemann，1992；Thiel et al.，1996)。BVDV区分成2个物种是基于基因组序列的明显不同(概述见Heinz et al.，2000)，从有限的交叉中和抗体反应也可以明显看出它们的区别(Ridpath et al.1994)。

III传统猪瘟病毒(CSFV)以前称作猪霍乱病毒，引起传统猪瘟(CSF)或猪霍乱(HC)(Moennig and Plagemann，1992；Thiel et al.，1996)。

IV边界病病毒(Border disease virus，BDV)一般发现于羊，引起边界病(BD)。BDV宫内感染小羊后，被持续感染的小羊可以出生但很虚弱并表现出不同的异常情况，其中最为人熟知的是“长毛摇摆(hairy shaker)”综合征(Moennig and Plagemann，1992；Thiel et al.，1996)。

瘟病毒是小的有包膜病毒，其正链单链RNA基因组既缺失5’帽也缺失3’poly(A)序列。该病毒基因组编码一种约有4000个氨基酸的聚蛋白，它通过细胞蛋白酶和病毒蛋白酶参与的翻译中加工和翻译后加工产生最终裂解产物。病毒蛋白在该聚蛋白中按NH₂-N^pro-C-E^rns-E1-E2-p7-NS2-NS3-NS4A-NS4B-NS5A-NS5B-COOH顺序排列(Lindenbach and Rice，2001)。蛋白C(＝核心蛋白或衣壳蛋白)以及糖蛋白E^rns、E1和E2代表瘟病毒颗粒的结构成分，已有人在CSFV中予以证明(Thiel et al.，1991)。BVDV中也是如此。有人发现E2是抗体中和的靶，E^rns也是但程度较低(Donis et al.，1988；Paton et al.，1992；van Rijn et al.，1993；Weiland et al.，1990，1992)。E^rns缺乏典型的膜锚着结构，可从感染的细胞大量分泌，有报道称这种蛋白表现出RNA酶活性(Hulst et al.，1994；Schneider et al.，1993；Windisch et al.，1996)。这种酶活性对于病毒生命周期的功能目前还不清楚。这种酶活性依赖于两个氨基酸链的存在，这两个链在瘟病毒E^rns以及植物来源和真菌来源的不同的已知RNA酶间是保守的。这两个保守序列都含有组氨酸残基(Schneider et al.，1993)。在CSFV疫苗株的E^rns蛋白中用赖氨酸替换这些残基中的一个导致破坏RNA酶活性(Hulst et al.，1998)。向CSFV疫苗株的基因组中导入这些突变不影响病毒活力或生长特性，但导致病毒表现出致细胞病变表型(Hulst et al.，1998)。相似地，Meyers等显示有毒的CSFV株Alfort/Tübingen的RNA酶阴性变体是有完整活力的。但这种病毒突变体不表现致细胞病变表型(Meyers et al.，1999)。

N^pro代表瘟病毒RNA的长开放读码框编码的第一个蛋白。N^pro代表非结构蛋白，其具有蛋白酶活性并推测在翻译期间已经将自身原始聚蛋白切割(Stark et al.，1993；Wiskerchen et al.，1991)。N^pro是半胱氨酸蛋白酶(Rümenapf et al.，1998)，其并非病毒复制所必需的(Tratschin et al.，1998)。最近有证据表明，N^pro在一定程度上干扰细胞的抗病毒防御功能，因此猜测它能在受感染的宿主内调节免疫系统(Rüggli et al.，2003)。Mayer和他的同事提出通过删除N^pro基因而使CSFV减毒(Mayer et al.，2004)。

目前用于预防和治疗BVDV感染的BVDV疫苗也有缺陷(Oirschot et al.1999)。抗传统BVDV-1的疫苗仅对BVDV-2感染提供部分保护，而已接种的牛(dam)会生出被有毒力的BVDV-2持续感染的小牛(Bolin et al.，1991，Ridpath et al.，1994)。这可能是由于1型和2型毒株间存在较大的抗原多样性，这种多样性在病毒中和的主要抗原糖蛋白E2中最显著(Tijssen et al.，1996)。大多数抗1型株的单克隆抗体不能结合2型病毒(Ridpath et al.，1994)。

包含减毒病毒或灭活病毒或在异源表达系统中表达的病毒蛋白的CSFV疫苗和BVDV疫苗已可以生产并且目前正在使用。灭活疫苗(灭活的完整病毒)或亚单位疫苗(传统纯化的或异源表达的病毒蛋白)产生完全的保护性免疫应答的效力常常不如活疫苗，即使在有佐剂存在时也是如此。

使BVDV减毒用作活疫苗的结构基础不清楚。这些疫苗尽管已减毒，仍常常涉及安全问题。疫苗病毒可以通过怀孕动物例如母牛的胎盘，在胎儿中引起临床表现，和/或产生被持续感染的小牛。因此，不能将它们用于包括怀孕的母牛在内的育种群体。怀孕的母牛必须与接种疫苗的牛隔离以保护胎儿并且其自身不能接种疫苗。而且，减毒活BVDV的回复体对动物形成严重威胁。传统衍生的减毒病毒通过传统的多次传代实现减毒，对它们而言，减毒现象的分子源由以及遗传稳定性仍不清楚，而且无法预测它们是否会回复为有毒力的野生型。

由于对瘟病毒感染实施有效、安全且可检测的预防和治疗的重要性，强烈需要改良的减毒瘟病毒(例如BVDV)以及包括所述减毒瘟病毒(例如BVDV)的组合物和疫苗，所述病毒具有诱导免疫的高潜力以及明确的减毒基础使之有别于致病性瘟病毒，例如BVDV，。

因此，本发明要解决的技术问题是提供改良的减毒瘟病毒(优选减毒BVDV)用作减毒活疫苗。这种改良的减毒瘟病毒，优选BVDV，应当尤其(i)本身不通过胎盘且(ii)可诱导免疫力，这种免疫力可防止病毒通过胎盘传播，并因此避免怀孕问题，例如在BVDV感染的情况下的胎儿流产问题或生出持续感染的宿主如小牛的问题。

附图简述

图1对NY93/C(II型BVDV)的血清中和的测定

图2对KE9(I型BVDV)的血清中和的测定

图3对NY93/C(II型BVDV)的血清中和的测定

所有后述序列用破折号(-)表示删除区，其也编号，序列表中的序列是连续编号的、不含删除区或氨基酸密码子。

SEQ ID NO：1 XIKE-A-cDNA序列

SEQ ID NO：2 XIKE-A-NdN-cDNA序列

SEQ ID NO：3 XIKE-B-cDNA序列

SEQ ID NO：4 XIKE-B-NdN-cDNA

SEQ ID NO：5 XIKE-A氨基酸序列

SEQ ID NO：6 XIKE-A-NdN氨基酸序列

SEQ ID NO：7 XIKE-B氨基酸序列

SEQ ID NO：8 XIKE-B-NdN氨基酸序列

SEQ ID NO：9 XIKE-C-NdN氨基酸序列

SEQ ID NO：10 XIKE-C-NdN-cDNA序列

SEQ ID NO：11 XIKE-C-cDNA序列

SEQ ID NO：12 XIKE-C氨基酸序列

发明概述

本发明涉及减毒瘟病毒，优选减毒BVDV，其中至少一个突变是在糖蛋白E^rns的编码序列中，且至少另一个突变在N^prp的编码序列中，优选这些突变导致除了灭活N^pro所带有的(推测的)免疫调节活性外，同时还灭活糖蛋白E^rns所带有的RNA酶活性。本发明也涉及使瘟病毒减毒的方法，所述减毒作用导致产生减毒的瘟病毒，优选减毒的BVDV，见上述。本发明还涉及编码所述减毒瘟病毒(优选减毒BVDV)的核酸分子，以及包含减毒瘟病毒(优选本文所述BVDV)的组合物和疫苗。

发明详述

说明书中使用的术语的定义：

在本发明的具体实施前必须指出，除非上下文有其它明确暗示，这里和所附的权利要求书中使用的单数形式“a”“an”和“the”包括复数意义。因此，例如，“a BVDV”包括该BVDV的复数，“细胞”的意思是一个或多个细胞以及本领域技术人员已知的所述细胞的等效形式，等等。除非另有定义，本文所有技术和科学术语具有本发明所属领域普通技术人员一般理解的相同含义。尽管可以将任何相似于或等同于本文所述的方法和材料用来实施或检验本发明，但是优选本文所述方法，装置和材料。本文所述的所有发表物在此引入作参考，以便描述和公开这些发表物中报道的、可以在与本发明相关联方面使用的细胞系、载体和方法学。不应将本文所述理解为承认本发明不具有利用在先发明提前公开这些内容的权利。

本文术语“瘟病毒”指黄病毒科中瘟病毒属的所有成员，包括BVDV，CSFV和BDV。

本文术语“CSFV”指属于黄病毒科瘟病毒属传统猪瘟病毒(classicalswine fever virus，CSFV)种的所有病毒。

本文术语“BVDV”指属于黄病毒科瘟病毒属中牛病毒性腹泻病毒(BVDV)1型(BVDV-1)和2型(BVDV-2)的所有病毒(Heinz et al.，2000)。较传统的BVDV 1型株和新近鉴别的BVDV 2型株在核苷酸和氨基酸序列中表现出一些有限但明显的不同。

本文中术语“N^pro”指病毒开放读码框编码的第一个蛋白，其将自身从合成的聚蛋白上切割下来而与该蛋白的其它部分脱离(Stark，et al.，J.Virol.67：7088-7093(1993)；Wiskerchen，et al.，Virol.65：4508-4514(1991))。根据上下文，该术语也可以指其编码核苷酸序列突变后保留的“N^pro”氨基酸，或编码该蛋白的核苷酸序列自身。“N^pro所带有的蛋白酶活性”指该“N^pro”的多肽裂解活性。

本文中“E^rns”指糖蛋白E^rns，其代表瘟病毒颗粒的一种结构成分(Thiel etal.，1991)。E^rns缺乏典型的膜锚结构，可以从感染的细胞大量分泌，据报道该蛋白表现出RNA酶活性(Hulst et al.，1994；Schneider et al.，1993；Windischet al.，1996)。应当指出在发表物中经常将术语糖蛋白E0用作糖蛋白E^rns的同义词。根据上下文，该术语也可以指其编码核苷酸序列突变后的“E^rns”蛋白或该蛋白的编码核苷酸序列自身。“糖蛋白E^rns所带有的RNA酶活性”指该糖蛋白的RNA裂解活性，即糖蛋白E^rns水解RNA的能力。术语“灭活该糖蛋白所带有的RNA酶活性”指与该糖蛋白E^rns的未修饰的野生型相比，修饰的糖蛋白E^rns不能水解RNA或水解能力减弱。

减毒：本文中“减毒的瘟病毒或BVDV颗粒”意思是，通过本文所述方法减毒的本发明减毒瘟病毒或BVDV颗粒与衍生所述减毒瘟病毒或BVDV颗粒的野生型瘟病毒或BVDV分离物，它们的毒力在主要临床参数方面有统计学显著差异，所述参数在BVDV的情况中是腹泻、发热和用同样剂量(优选6×10⁶TCID₅₀)感染的动物的致死率。因此，所述减毒BVDV颗粒不引起腹泻、发热和死亡并因此可在疫苗中使用。

本文中灭活E^rns是指，在Meyers等1999所述RNA酶测定中，RNA酶活性不明显高于未感染的对照细胞测得的水平。“在Meyers等1999所述RNA酶测定中，不明显高于未感染的对照细胞测得的水平”意思是例如，RNA酶活性比未感染的对照细胞低150％。

本文中灭活N^pro是指，通过突变来阻碍或明显减弱N^pro的可能的免疫调节活性。在优选实施方案中，这种突变阻碍或大大减弱N^pro对受染细胞诱导干扰素应答的作用的干扰，参见Rüggli等(2003)。在这种情况下，灭活N^pro将允许所述细胞进行(mount)正常的干扰素应答。

本文中“加工信号”指确保产生瘟病毒(优选BVDV)C蛋白的功能性N-末端的物质，特别是选自泛素、LC3、SUMO-1、NEDD8、GATE-16和GABA(A)RAP的物质。选自内含肽(intein)、小RNA病毒3C、心病毒(caridovirus)2A和兔出血性疾病病毒p15的蛋白酶在本文中也理解为“加工信号”。术语“加工信号”也包括技术人员已知的保证C蛋白的功能性N-末端产生的任何其它相似的加工信号。

本文中“蛋白C”或“C蛋白”或“C-蛋白”指瘟病毒颗粒的一种结构成分(Thiel et al.，1991)。“蛋白C”是瘟病毒的衣壳或核心蛋白。根据上下文，该术语也可以指带有因编码核苷酸序列突变所致一或多个氨基酸替换的“蛋白C”。

本发明中“片段”是本发明多核苷酸分子的任何亚单位，即任何亚群(subset)。在DNA的情况中，所述片段是比覆盖全长病毒基因组的DNA更短的DNA。

本发明核苷酸分子的“功能性变体”是指，具有与本发明核苷酸分子基本相似生物学活性(指功能活性或结构活性)的核苷酸分子。术语“功能性变体”也包括“片段”、“基于简并核酸密码的变体”或“化学衍生物”。这种“功能性变体”例如可以携带一或多个核苷酸替换、删除或插入。该功能性变体至少部分保留其生物学活性，例如作为感染性克隆或疫苗株的功能，或者甚至表现出改进的生物学活性。“具有基本相似生物学活性”是指本文所述瘟病毒而言，例如，所述瘟病毒按本文所述方式减毒，并导致产生适于制备减毒活病毒的非致病性病毒，其丧失通过胎盘的能力但是在接种后介导免疫应答。

“基于遗传密码简并特性的变体”是因一种氨基酸可以由几种不同的核苷酸三联体编码的事实导致的变体。所述变体至少部分保留其生物学活性，或者甚至表现出改进的生物学活性。

如果两种分子具有基本相似的核苷酸序列或生物学活性，则其中一种分子“基本相似于”另一分子。因此，只要两种分子具有相似活性，即使它们的核苷酸序列不相同也认为它们是本文所述变体，两种具有相似核苷酸序列的分子即使它们的生物学活性不相同也认为是本文所述变体。

本文中突变指编码本发明蛋白/氨基酸的核酸分子中的修饰。所述突变涉及但不限于：取代(置换一或多个核苷酸/碱基对)，删除(去除一或多个核苷酸/碱基对)，和/或插入(添加一或多个核苷酸/碱基对)。本文中突变可以是单一突变或多个突变，因此，术语“突变”在本文中常用，它既指单一突变也指多个突变。所述突变包括但不局限于：点突变(单个核苷酸突变)或较大突变，其中例如编码性核酸分子的部分被删除、取代和/或插入了额外的编码性核酸。所述突变可以因编码序列中的改变而表达修饰的多肽。这种修饰的多肽是所需的，正如本发明下文中所述。

本文中术语“疫苗”指药用组合物，其包含至少一种在动物体内诱导免疫应答的免疫活性成分，并且可以但非必需包含一种或多种增强该活性成分的免疫活性的额外成分。疫苗还可包含药用组合物常用的其它成分。疫苗的免疫活性成分可以包含完整病毒颗粒，所述颗粒可以是其原始形式，或者是在改良活疫苗(MLV)中的减毒颗粒，或者是在灭活疫苗(KV)中经适当方法灭活的颗粒。在另一种形式中，疫苗的免疫活性成分可以包含所述生物的适当成分(亚单位疫苗)，这些成分可通过破坏整个颗粒或者含有这种颗粒的生长培养物来产生，并可选随后纯化产生所需结构，或者这些成分可通过合成产生，包括利用基于细菌、昆虫、哺乳动物或其它物种的合适系统进行适当操作并可选随后进行分离和纯化，或者这些成分可通过在需要疫苗的动物中诱导该合成过程而产生，方法是用合适的药用组合物直接掺入遗传物质(多核苷酸接种)。疫苗可以包含一种上述成分或同时包含一种以上的上述成分。本文中术语“疫苗”指兽医使用的含抗原物质的疫苗，其给药目的是，诱导特异性抵抗由瘟病毒感染(优选BVDV感染)引发的疾病的主动免疫。本文所述减毒瘟病毒、特别是减毒BVDV可提供主动免疫力，这种免疫力可以经由母体抗体被动转移，所述母体抗体是指抵抗该病毒所含免疫原以及有时也抵抗抗原相关生物的抗体。本发明的疫苗是指上述疫苗，其中一种免疫活性成分是BVDV、或者是来源于瘟病毒、或者是衍生自与任何已知瘟病毒序列(正义或反义)70％以上同源的核苷酸序列。

术语“活疫苗”指包括活的、特别是活的病毒活性成分的疫苗。

增强免疫应答的额外成分是一般称作“佐剂”的组分，例如氢氧化铝、矿物油或其它油、或者是添加到所述疫苗中或在这类额外成分各自诱导后由机体产生的辅助分子，例如但不局限于干扰素、白细胞介素或生长因子。

“药用组合物”基本由一种或多种能改变生物的生理功能(例如免疫功能)的成分组成，所述生物是指被给药的生物、或者生活在该成分中的生物、或者有该成分生活于其上的生物。该术语包括但不局限于抗生素或抗寄生虫药，以及常用于达到其它目的的其它组分，这些目的例如但不局限于：加工特性，无菌性，稳定性，经肠道或非肠道途径(例如口服，鼻内，静脉内，肌肉内，皮下，皮内或其它合适途径)给药的可行性，给药后的耐受性，控制释放特性。仅仅为证明的目的而给出的这种药用组合物的一个非限制性例子可以如下制备：将受染细胞培养物的培养上清与稳定剂(例如亚精胺和/或BSA(牛血清白蛋白)混合，随后将混合物冻干或用其它方法脱水。接种前，将该混合物在水溶液(例如盐水，PBS(磷酸盐缓冲液)中或非水溶液(例如油乳剂、基于铝的佐剂)中水化。

发明内容

解决上述技术问题的技术方案可参见说明书和权利要求中具体表征的实施方案。

申请人惊讶发现，瘟病毒，特别是BVDV，可通过在糖蛋白E^rns编码序列中引入至少一个突变并在N^pro编码序列中引入至少另一个突变而被更有效地减毒，所述突变优选导致除了灭活N^pro所带有的免疫调节活性外还灭活E^rns所带有的RNA酶活性。免疫调节作用在一个方面是指一种瘟病毒的指定功能，但不局限于此，例如参见Rüggli等(2003)。

按照本发明的减毒瘟病毒(特别是BVDV)在疫苗中使用较有利。所述减毒瘟病毒(特别是减毒BVDV)提供了强免疫原性的活疫苗。申请人惊讶发现，本发明的瘟病毒(特别是BVDV)在怀孕的动物中使用也是安全，因为它们不能通过胎盘。这可参见实施例3中对BVDV的举例说明。

此外，具有作为减毒基础的指定突变的活疫苗将允许避免目前疫苗制备的缺点，即回复为致病性更强的毒株的风险。所述减毒突变的另一优势在于其分子独特性，这种独特性使得它们能被用作减毒瘟病毒(特别是BVDV)的特有标记，并将这些病毒与本领域的其它瘟病毒(特别是BVDV)区分开来。因此，本发明一方面提供减毒瘟病毒(特别是减毒BVDV)，其在糖蛋白E^rns编码序列中有至少一个突变并在N^pro编码序列中有至少另一个突变。优选，在这种减毒瘟病毒中(优选在这种减毒BVDV中)，所述在糖蛋白E^rn的编码序列中的突变导致E^rns所带有的RNA酶活性被灭活，和/或所述在N^pro编码序列中的突变导致该N^pro被灭活。所述灭活可以通过本领域技术人员已知的E^rns-和N^pro-编码序列的任何突变来实现，其中所述突变是“定义”章节定义的任何突变，例如删除，插入突变和/或取代突变。更优选，所述突变是删除，因为回复成野生型的可能性在删除的情况中是最小的。

已知糖蛋白E^rns形成大约97kD的由二硫键连接的同二聚体，其中每一单体由相当于Rümenapf等(1993)所述CSFV聚蛋白氨基酸268至494的227个氨基酸组成。CSFV Alfort/Tübingen株的基因组序列可从GenBank/EMBL数据库获得，登录号J04358；BVDV株CP7的氨基酸序列在GenBank/EMBL数据库可获得(登录号U63479)；在BVDV CP7聚蛋白中，E^rns蛋白相当于残基271至497。在糖蛋白E^rns以及一些植物来源和真菌来源的具有RNA酶活性的蛋白中，这两个氨基酸区高度保守(Schneider et al.，1993)。这两个区对RNA酶的酶促活性特别重要。在Meyers等1999所述CSFV Alfort株聚蛋白的情况中，第一个区域由该病毒聚蛋白的295至307位氨基酸所示区域组成(在BVDV cp7株的情况中为298至310)，第二个区域由该病毒聚蛋白的338至357位氨基酸所示区域组成(在BVDV cp7株的情况中为341至360)(编号参照已公开的CSFV株Alfort/Tübingen的公认氨基酸序列(Meyerset al.，1989)。如上面提到的对RNA酶活性特别重要的氨基酸绝不限于CSFVAlfort/Tübingen株中的上述指定位点，这是用来简单举例方式以指出优选的氨基酸在该位点，或由于它们是高度保守的，因此在其它株例如在BVDV，BDV和瘟病毒的相当于所述位点处。对除了CSFV Alfort/Tübingen株以外的瘟病毒而言，优选的氨基酸的位置编号可以不同，但是瘟病毒分子生物学领域的专家可通过该氨基酸序列的高度保守和在前后序列中这些基序的位置而容易地鉴别出这些优选的氨基酸。在一个具体的非限制性例子中，CSFV Alfort/Tübingen株的位点346等同于BVDV cp7株的位点349。

本发明因此优选涉及本发明BVDV，其中糖蛋白E^rns编码序列中的所述突变位于与氨基酸位点298至310和/或位点341至360对应的编码核苷酸序列中。优选，所述突变是(单字母符号表示的氨基酸；位置编号之前的氨基酸表示将要被取代的氨基酸，位置编号之后的氨基酸表示取代后的氨基酸(del表示删除)：例如，H300L表示组氨酸300被亮氨酸取代：

糖蛋白E^rns的合适修饰是例如单取代/删除：S298G，H300K，H300L，H300R，H300del，W303G，P304del，E305A，C308G，R343G，E345del，W346G，K348A，H349K，H349L，H349del，H349Q，H349SV(突变H349S和插入V)，K348R，W351P，W351G，W351L，W351K，W351H；双取代/删除：H300L/H349L，K348del/H349del，H349del/G350del，E345del/H349del，W303G/E305A，H300K/H349K，H300K/H349L和三删除：L299del/H300del/G300del，K348del/H349del/G350del。对于上面列出的突变，按照发表的BVDV CP7的氨基酸序列编号(编号减3相当于CSFVAlfort/Tübingen氨基酸序列的等同残基)。经测试，所有上面列出的突变至少分别为不含N^pro区突变的CSFV或BVDV突变体。瘟病毒糖蛋白E^rns的合适突变体在例如WO 99/64604中描述，该文献在此全部引入以作参考。但应指出，按照本发明，N^pro区必须有至少一个额外突变，详见下文。

申请人发现，删除或取代位点346(CSFV)或349(BVDV)的组氨酸残基导致有效灭活E^rns，并因此获得特别有用的瘟病毒活疫苗。本发明证明，病毒聚蛋白的位点346(按照发表的CSFV Alfort/Tübingen(Meyers et a1.，1989)的序列编号)或该瘟病毒是BVDV时的位点349(按照发表的BVDV CP7(Meyers et al.，1996b)的序列编号)的组氨酸残基代表E^rnsRNA酶的保守且公认的活性位点残基，当该残基被删除时，瘟病毒是有活力的并编码无RNA酶活性的E^rns蛋白。因此，优选，本发明也涉及本发明BVDV，其中所述在糖蛋白E^rns编码序列中的突变是位点349组氨酸残基的删除或取代。甚至更具体地，公认的RNA酶活性位点是保守的E^rns序列SLHGIWPEKICTG和/或LQRHEWNKHGWCNWFHIEPW(这里给出BVDV-2 New York’93蛋白的序列作为举例；在其它瘟病毒序列中可以发现微小改变，但是所述基序的特征对本领域专家而言是明显的。例如，BVDV-1 CP7的相应氨基酸序列是SLHGIWPEKICTG和/或LQRHEWNKHGWCNWYNIEPW，CSFVAlfort/Tüibingen的相应氨基酸序列是SLHGIWPEKICKG和/或LQRHEWNKHGWCNWYNIDPW)。因此，优选，本发明还涉及本发明BVDV，其中所述在糖蛋白E^rns编码序列中的突变位于编码保守的E^rns序列SLHGIWPEKICTG和/或LQRHEWNKHGWCNWFHIEPW的核苷酸序列中。这些序列代表公认的RNA酶活性位点。公认的E^rns活性位点的序列SLHGIWPEKIC和RHEWNKHGWCNW在各种瘟病毒之间更为保守。因此，优选，本发明涉及在N^pro蛋白和糖蛋白E^rns的编码序列中有至少一个突变的瘟病毒，特别是BVDV，其中所述在糖蛋白E^rns编码序列中的突变位于编码保守的E^rns序列SLHGIWPEKIC和/或RHEWNKHGWCNW的核苷酸序列中，优选，所述突变仅位于一种所述序列中。因此本发明也涉及在N^pro蛋白和糖蛋白E^rns的编码序列中有至少一个突变的瘟病毒，特别是BVDV，其中所述在糖蛋白E^rms编码序列中的突变位于编码保守的E^rns序列SLHGIWPEKIC和/或HEWNKHGWCNW的核苷酸序列中，优选，所述突变涉及两个不同的氨基酸，即为双突变。因此，所述突变可以是编码两个氨基酸的两个不同三联码中1-3个核苷酸的突变。因此本发明也涉及在N^pro蛋白和糖蛋白E^rns的编码序列中有至少一个突变的瘟病毒，特别是BVDV，其中所述在糖蛋白E^rns编码序列中的突变是位于编码保守的E^rns序列SLHGIWPEKIC和/或RHEWNKHGWCNW的核苷酸序列中的双突变。优选，所述突变涉及单个氨基酸。因此，所述突变可以是编码一个氨基酸的一个三联码中1-3个核苷酸的突变。因此本发明也涉及在N^pro蛋白和糖蛋白E^rns的编码序列中有至少一个突变的瘟病毒，特别是BVDV，其中在保守的E^rns序列SLHGIWPEKIC和/或RHEWNKHGWCNW中有单个突变。

如上所述，本发明提供了在糖蛋白E^rns编码序列和N^Pro蛋白编码序列中有至少一个突变的减毒瘟病毒，其中所述突变优选导致灭活糖蛋白E^rns所带有的RNA酶活性和N^pro所带有的免疫调节活性。在瘟病毒，特别是下文祥述的具体通式所示的BVDV中，N^pro被灭活，在所述通式中，具有N^pro的0-所有氨基酸；具有或不具有泛素或LC3或另一个序列作为加工信号(例如SUMO-1，NEDD8，GATE-16，GABA(A)RAP，或蛋白酶例如内含肽，小RNA病毒3C，心病毒2A或兔出血性疾病病毒的p15)。在有加工信号的情况中，将该加工信号的编码序列插入N^pro-蛋白(其剩余部分)C-末端或其附近。仅在有加工信号时，可以有任何数目的编码N^pro的氨基酸(＝N^pro氨基酸)。当不插入加工信号序列时，可以有最多12个N^pro氨基酸，优选其氨基末端氨基酸，其余氨基酸必须删除。而且，除上述E^rns突变(至少其中有一种存在于本发明瘟病毒特别是BVDV中)外，瘟病毒(特别是BVDV)的其余序列仍保持不变，即不突变，或者也可以在C-蛋白的N-末端附近有突变。下文给出了这方面的更具体实施方案。

因此，本发明涉及瘟病毒，特别是本发明BVDV，其中所述在N^pro编码序列中的突变导致编码的聚蛋白有下列通式的特征：

[N^pro]_x-[PS]_y-[C-term]

并且其中：

[N^pro]涉及该聚蛋白的N^pro部分，其中“x”代表该聚蛋白中的N^pro氨基酸数。

[PS]涉及选自下列的加工信号：泛素、LC3、SUMO-1、NEDD8、GATE-16或GABA(A)RAP或蛋白酶例如内含肽、小RNA病毒3C、心病毒2A或兔出血性疾病病毒的p15，或技术人员已知确保产生C蛋白功能性N-末端的任何加工信号。“Y”可以是＝0，其意味着不存在加工信号(＝无PS)，或“Y”可以是＝1，其意味着存在加工信号(＝有PS)。

[C-term]涉及完整瘟病毒(特别是完整BVDV)的聚蛋白，其不含N^pro，但包含衣壳(C)-蛋白和瘟病毒聚蛋白(尤其BVDV聚蛋白)中的任何其它蛋白，包括羧基端NS5B。优选，所述[C-term]中的糖蛋白E^rns有突变，以致糖蛋白E^rns的RNA酶活性失活。术语“瘟病毒聚蛋白/BVDV聚蛋白中的任何其它蛋白”涉及E^rns，E1，E2，p7，NS2，NS3，NS4A，NS4B和NS5A，其中糖蛋白E^rns有突变，优选如本文所述(见上文)，致使糖蛋白E^rns的RNA酶活性失活。优选，本发明瘟病毒(特别是BVDV)具有C-蛋白，该蛋白除了位点2的氨基酸从D变成N外不含有其它突变。因此，[C-term^*]等同于[C-term]但是在C-蛋白的位点2有突变(N取代D)；

当“y”＝0(意味着无[PS]存在)那么“x”是0至12，(意味着无N^pro特异氨基酸或有N^pro的1至12个氨基酸，优选有N^pro的N-末端)；

当“y”＝1(意味着存在[PS])那么“x”是0至168；(意味着无N^pro特异氨基酸或有N^pro的全部1至168个氨基酸，优选有N^pro的N-末端)。

也更优选，本发明涉及瘟病毒，特别是本发明BVDV，其中所述在N^pro编码序列中的突变导致编码的聚蛋白具有下列通式的特征：

[N^pro]₁-[PS]₀-[C-term]

并且其中定义如上所述。

其具体例子在下面公开，其中N-末端的蛋氨酸后跟随C-蛋白以及该聚蛋白中的任何其它蛋白，包括羧基端NS5B。因此，最优选，本发明涉及瘟病毒，特别是本发明BVDV，其中所述在N^pro编码序列中的突变导致编码的聚蛋白具有下列通式的特征：

M[C-term]。

并且其中定义如上所述。

[N^pro]₃-[PS]₀-[C-term]

并且其中定义如上所述。

下面公开了BVDV的具体例子，其中N-末端的蛋氨酸后跟随N^pro序列EL和C-蛋白以及该聚蛋白中的任何其它蛋白，包括羧基端NS5B。因此，最优选，本发明涉及本发明BVDV，其中所述在N^pro编码序列中的突变导致编码的聚蛋白具有下列通式的特征：

MEL-[C-term]

并且其中定义如上所述。

[N^pro]₄-[PS]₀-[C-term]

并且其中定义如上所述。

下面公开了BVDV的具体例子，其中N-末端的蛋氨酸后跟随N^pro序列ELF和C-蛋白以及该聚蛋白中的任何其它蛋白，包括羧基端NS5B。因此，最优选，本发明涉及本发明BVDV，其中所述在N^pro编码序列中的突变导致编码的聚蛋白具有下列通式的特征：

MELF-[C-term].

并且其中定义如上所述。

[N^pro]₆-[PS]₀-[C-term]

并且其中定义如上所述。

下面公开了BVDV的具体例子，其中N-末端的蛋氨酸后跟随N^pro序列ELFSN和C-蛋白以及该聚蛋白中的任何其它蛋白，包括羧基端NS5B。因此，最优选，本发明涉及本发明BVDV，其中所述在N^pro编码序列中的突变导致编码的聚蛋白具有下列通式的特征：

MELFSN-[C-term].

并且其中定义如上所述。

[N^pro]₄-[PS]₀-[C-term^*]

并且其中除了改变C-蛋白的氨基末端部分外，定义如上所述。

下面公开了BVDV的具体例子，其中N-末端的蛋氨酸后跟随N^pro序列ELF，并且在C-蛋白序列中位点2的氨基酸从D变成N。因此，氨基末端的C-蛋白序列是SNEGSK...而不是SDEGSK。因此，最优选，本发明涉及本发明BVDV，其中所述在N^pro编码序列中的突变导致编码的聚蛋白具有下列通式的特征：

MELF-[C-term^*]，

其中在C-蛋白位点2的氨基酸从D变成N，并且其中定义如上所述。

[N^pro]_x-[PS]₁-[C-term]，

并且其中定义如上所述，

并且其中PS是上述任一种PS，优选选自泛素或LC3。

下面公开了BVDV的具体例子，其中N-末端的蛋氨酸后跟随任何21个或28个N^pro氨基酸、泛素或LC3和C-蛋白。因此，最优选，本发明涉及本发明BVDV，其中所述在N^pro编码序列中的突变导致编码的聚蛋白具有下列通式的特征：

[N^pro]₂₂-[PS]₁-[C-term]，其中优选，PS是泛素或LC3或者

[N^pro]₂₉-[PS]₁-[C-term]，其中优选，PS是泛素或LC3。

泛素是众所周知的高度保守的细胞蛋白，有76个氨基酸。泛素有多种功能，其中之一是在蛋白质代谢中起关键作用，因为与泛素偶联可以使蛋白被标记以便由蛋白酶体降解。泛素通过其羧基端甘氨酸与其它蛋白偶联或融合后，该泛素可以被细胞的泛素特异性蛋白酶切除。因此，蛋白与泛素羧基端融合通常会导致该融合蛋白在细胞内表达后在指定部位被蛋白水解，从而得到其组成成分。

LC3(微管相关蛋白的轻链3)代表一种具有多功能的细胞蛋白，其有125个氨基酸(指牛LC3的长度)。最近，已经确定该蛋白在自吞噬中的基本作用。在此过程中，LC3因羧基端切割而激活。由此产生由甘氨酸组成的新羧基末端。然后LC3借羧基末端甘氨酸与自吞噬小泡膜上的磷脂酰乙醇胺偶联。由于此过程，与LC3羧基端融合的蛋白被细胞的蛋白酶在指定位点切割。

也更优选，本发明涉及瘟病毒，优选本发明BVDV，其中所述在N^pro编码序列中的突变导致编码的聚蛋白具有下列通式的特征：

[N^pro]₂-[PS]_y-[C-term]并优选ME-[PS]_y-[C-term]；

[N^pro]₅-[PS]_y-[C-term]并优选MELFS-[PS]_y-[C-term]；

[N^pro]₇-[PS]_y-[C-term]并优选MELFSNE-[PS]_y-[C-term]；

[N^pro]₈-[PS]_y-[C-term]并优选MELFSNEL-[PS]_y-[C-term]；

[N^pro]₉-[PS]_y-[C-term]并优选MELFSNELL-[PS]_y-[C-term]；

[N^pro]₁₀-[PS]_y-[C-term]并优选MELFSNELLY-[PS]_y-[C-term]；

[N^pro]₁₁-[PS]_y-[C-term]并优选MELFSNELLYK-[PS]_y-[C-term]；和

[N^pro]₁₂-[PS]_y-[C-term]并优选MELFSNELLYKT-[PS]_y-[C-term]

并且其中定义如上所述。优选公开的实施方案指BVDV。

最优选，y是0(无PS存在)。

也更优选，如上面描述的本发明BVDV是BVDV 1型BVDV。最优选，如上面描述的本发明BVDV是BVDV 2型BVDV。BVDV-1和BVDV-2按照其基因组序列区分(Heinz et al.，2000和其参考文献)。本文所述BVDV-1可以用于制备组合物，所述组合物用于在育种牛群、怀孕母牛中预防和/或治疗BVDV-1感染，以及用于在怀孕母牛中诱导对抗BVDV 1型感染的胎儿保护作用。意外的是，本文所述BVDV-2可以用于制备在育种牛群中预防和/或治疗BVDV-1感染时使用的组合物。特别地，本发明涉及本发明2型BVDV用于制备在怀孕母牛中预防和/或治疗BVDV 1型感染时使用的组合物的用途。优选，本发明BVDV 2型可用于制备在怀孕母牛中诱导对抗BVDV 1型感染的胎儿保护作用的组合物。同样意外的是，本文所述BVDV-1可以用于制备在育种牛群中预防和/或治疗BVDV-2感染时使用的组合物。特别地，本发明涉及本发明BVDV 1型用于制备在怀孕母牛中预防和/或治疗BVDV 1型感染时使用的组合物。优选，本发明BVDV 1型可以用于制备在怀孕母牛中诱导对抗BVDV 2型感染的胎儿保护作用的组合物。最优选BVDV 1型和2型组合用于制备组合物，所述组合物用于在育种牛群、怀孕母牛中预防和/或治疗BVDV-1型和/或2型感染，以及在怀孕母牛中诱导对抗BVDV 1型和/或2型感染的胎儿保护作用。

最优选，将要按本文所述进行突变的本发明野生型BVDV相当于SEQID No.5(称作XIKEA)的氨基酸序列或是其功能变体。也最优选，本发明BVDV具有本发明N^pro突变并相当于SEQ ID No.6(称作XIKE-A-NdN)的氨基酸序列或是其功能变体。优选，这种功能变体至少65％同源于本文公开的氨基酸序列。在氨基酸水平，同源性是非常粗略(roughly)的：BVDV-1/-BVDV-1：93％；BVDV-1/-BVDV-2：84％；BVDV-2/-BVDV-2：98％。因此，更优选，这种功能变体与本文公开的氨基酸序列同源性至少70％，71％，72％，73％，74％，75％，76％，77％，78％，79％，80％，81％，82％，83％，84％，85％，86％，87％，88％，89％或90％。也更优选，这种功能变体与本文公开的氨基酸序列同源性至少91％，92％，93％，94％，95％，96％，97％或98％。最优选，这种功能变体与本文公开的氨基酸序列同源性至少99％或99.9％。

也最优选，本发明BVDV具有本发明所述E^rns突变(删除了编码组氨酸349的密码子)，它相当于SEQ ID No.7(称作XIKE-B)的氨基酸序列或是其功能变体。也最优选，本发明BVDV具有本发明的E^ms突变和N^pro突变两者，其中删除了编码E^rns中组氨酸349的密码子，还删除了除密码子1-4以外的完整N^pro编码区，因此保留了N^pro的氨基酸MELF。所述突变体相当于SEQ ID No.8(称XIKE-B-NdN)的氨基酸序列或是其功能变体。优选，这种功能变体至少65％与本文公开的氨基酸序列同源。更优选，这种功能变体与本文公开的氨基酸序列同源性至少70％，71％，72％，73％，74％，75％，76％，77％，78％，79％，80％，81％，82％，83％，84％，85％，86％，87％，88％，89％或90％。也更优选，这种功能变体与本文公开的氨基酸序列同源性至少91％，92％，93％，94％，95％，96％，97％或98％。最优选，这种功能变体与本文公开的氨基酸序列同源性至少99％或99.9％。

也最优选，本发明BVDV具有本发明E^rns突变(编码组氨酸300的密码子被编码亮氨酸的密码子取代)，并相当于SEQ ID No.9(称作XIKE-C)的氨基酸序列或是其功能变体。也最优选，本发明BVDV具有本发明E^rns突变和N^pro突变两者，其中编码组氨酸300的密码子被编码亮氨酸的密码子取代，另外还删除了除密码子1-4以外的完整N^pro编码区，因此保留了N^pro的氨基酸MELF。所述突变体相当于SEQ ID No.10(称作XIKE-C-NdN)的氨基酸序列或是其功能变体。优选，这种功能变体至少65％同源于本文公开的氨基酸序列。更优选，这种功能变体与本文公开的氨基酸序列同源性至少70％，71％，72％，73％，74％，75％，76％，77％，78％，79％，80％，81％，82％，83％，84％，85％，86％，87％，88％，89％或90％。也更优选，这种功能变体与本文公开的氨基酸序列同源性至少91％，92％，93％，94％，95％，96％，97％或98％。最优选，这种功能变体与本文公开的氨基酸序列同源性至少99％或99.9％。

本文所述发明的另一重要实施方案是组合物，其包含本发明瘟病毒(特别是BVDV)和溶液。技术人员知道可以包含在所述组合物中的额外成分(也参见Remington′s Pharmaceutical Sciences.(1990).18th ed.Mack Publ.，Easton)。专家可以使用已知的可注射且生理可接受的无菌溶液。制备非肠道注射或输注的随时可用的溶液时，可以很容易获得等渗水溶液例如盐水或相应的血浆蛋白溶液。药用组合物可以以冻干物或干燥制剂形式提供，其在使用前在无菌条件下用已知的可注射的溶液直接重建，例如为组配试剂盒形式(a kit of parts)。

本发明组合物的最终制剂可以制备为例如注射所用的制剂，方法是，将本发明瘟病毒(优选BVDV)与生理可接受的无菌溶液混合，可以添加已知的载体物质和/或添加剂(例如血清白蛋白，葡萄糖，亚硫酸氢钠，EDTA)。这种溶液可以基于生理可接受的溶剂，例如pH7-8的水溶液。pH可以通过可药用的缓冲液来稳定。所述溶液还可以含有稳定剂如表面活性剂例如吐温20，血清白蛋白例如BSA(牛血清白蛋白)，抗坏血酸，和/或亚精胺。所述组合物也可以含有佐剂，例如氢氧化铝、矿物油或其它油、或者是添加到所述疫苗中或在这类额外成分各自诱导后由机体产生的辅助分子，例如但不限于，干扰素，白细胞介素或生长因子。

例如，在本发明组合物中，可以将瘟病毒(特别是BVDV)溶于：

瘟病毒(优选BVDV) 10²-10⁸ TCID50

SGS^* 25％v/v

细胞培养基 qsp 1剂

^*SGS：每2毫升中的组成

蔗糖 75mg

明胶 20mg

氢氧化钾 0.274mg

L-谷氨酸 0.72mg

磷酸二氢钾 0.516mg

磷酸氢二钾 1.254mg

注射用水 qsp 2ml

当所述组合物事先已用其它方法冻干或脱水时，在接种前，将所述组合物在水溶液(例如盐水，PBS(磷酸盐缓冲液))或非水溶液(例如油乳剂(基于矿物油、或植物油/可代谢的油/基于单或双乳剂)，基于铝的佐剂，基于卡波姆(carbomer)的佐剂)中再水化。

优选，本发明组合物在动物中诱导免疫应答。更优选，本发明组合物是疫苗。本文所理解的疫苗包含瘟病毒，特别是本发明BVDV，定义如上述(见“定义”章节)。

最优选，本发明组合物还包括可药用的载体或赋形剂。上文公开了几种载体或赋形剂。如果所述组合物是用于注射或输注，可以包括制备等渗溶液所用的物质，保存剂例如p-羟苯甲酸，稳定剂例如乙二胺四乙酸的碱金属盐，可能也含有乳化剂和/或分散剂。

本发明组合物可以皮内、气管内或阴道内应用。优选将组合物在肌肉内或鼻内应用。在动物体内，可以证实上文所述药用组合物经静脉内施用或直接注射到靶组织中有优势。在全身应用的情况中，优选静脉内，血管内，肌肉内，鼻内，动脉内，腹膜内，口服，或鞘内途径。更局部的应用可以是皮下，真皮内(intradermally)，皮内(intracutaneously)，心内，小叶内(intralobally)，髓内(intramedullarly)，肺内或直入待处理的组织(结缔组织，骨组织，肌肉组织，神经组织，上皮组织)中或该组织附近。根据所需持续时间和治疗效果，本发明组合物可以给药一次或多次，或间歇给药，例如每天给药且持续数日、数周或数月并以不同剂量给药。

本发明也涉及本发明瘟病毒，特别是BVDV，在制备用于预防和治疗瘟病毒感染特别是BVDV感染的疫苗中的用途。

本发明的另一重要部分是含有编码本发明瘟病毒(特别是BVDV)的核酸的多核苷酸分子，或片段，功能变体，基于简并核酸密码子的变体，融合分子或其化学衍生物。优选，所述多核苷酸分子是DNA。也优选，所述多核苷酸分子是RNA。在更优选的实施方案中，所述多核苷酸分子也包括瘟病毒特别是BVDV的功能性5’-和/或3’-非翻译区的核苷酸序列。

本领域已知有多种核苷酸序列可以作为制备编码本发明减毒瘟病毒的多核苷酸分子的基础，所述的本发明减毒瘟病毒在N^pro编码序列中有至少一个突变且在糖蛋白E^rns编码序列中有至少一个突变，所述突变导致既灭活糖蛋白E^rns所带有的RNA酶活性也灭活N^pro所带有的免疫调节活性。下面列出瘟病毒多个成员的野生型序列的核酸序列例子：

边界病病毒

BD31株 NCBI GenBank登录号[U70263]

X818株 NCBI GenBank登录号[AF037405]

牛病毒性腹泻病毒1

NADL株 NCBI GenBank登录号[M31182]

Osloss株 NCBI GenBank登录号[M96687]

SD-1株 NCBI GenBank登录号[M96751]

CP7株 NCBI GenBank登录号[U63479]

牛病毒性腹泻病毒2

890株 NCBI GenBank登录号[U18059]

C413株 NCBI GenBank登录号[AF002227]

传统猪瘟病毒

Alfort/187株 NCBI GenBank登录号[X87939]

Alfort-Tübingen株 NCBI GenBank登录号[J04358]

Brescia株 NCBI GenBank登录号[M31768]

C株 NCBI GenBank登录号[Z46258]

本发明涉及N^pro和E^rns编码序列的突变/修饰在上文中祥述。基于这些信息，本领域技术人员能制备编码本发明瘟病毒的任何多核苷酸/多核酸。而且，此人能制备本发明的减毒瘟病毒。用于在多核苷酸序列中导入突变、克隆和扩增所述突变的多核苷酸的分子方法可以参见例如Sambrook等1989或Ausubel等1994。

最优选，将要按本文所述进行突变的本发明野生型BVDV由核酸序列SEQ ID No.1(称作XIKE A)或其功能变体编码。也最优选，本发明BVDV具有本发明N^pro突变并由SEQ ID No.2(称作XIKE-A-NdN)或其功能变体编码。优选，这种功能变体至少65％同源于本文公开的核酸序列。在核酸水平，同源性是非常粗略的：BVDV-1/-BVDV-1：80％；BVDV-1/-BVDV-2：70％；BVDV-2/-BVDV-2：96％。因此，更优选，这种功能变体与本文公开的核酸序列同源性至少70％，71％，72％，73％，74％，75％，76％，77％，78％，79％，80％，81％，82％，83％，84％，85％，86％，87％，88％，89％或90％。也更优选，这种功能变体与本文公开的核酸序列同源性至少91％，92％，93％，94％，95％，96％，97％或98％。最优选，这种功能变体与本文公开的核酸序列同源性至少99％或99.9％。

也最优选，本发明BVDV具有本发明所述E^rns突变(删除了编码H349的密码子)，它由SEQ ID No.7(称作XIKE-B)或其功能变体编码。也最优选，本发明BVDV具有本发明的E^rns突变和N^pro突变两者，其中删除了编码E^rns中组氨酸349的密码子，还删除了除密码子1-4以外的完整N^pro编码区，因此保留了N^pro的氨基酸MELF。所述突变由SEQ ID No.8(称作XIKE-B-NdN)或其功能变体编码。优选，这种功能变体至少65％与本文公开的核酸序列同源。更优选，这种功能变体与本文公开的核酸序列同源性至少70％，71％，72％，73％，74％，75％，76％，77％，78％，79％，80％，81％，82％，83％，84％，85％，86％，87％，88％，89％或90％。也更优选，这种功能变体与本文公开的核酸序列同源性至少91％，92％，93％，94％，95％，96％，97％或98％。最优选，这种功能变体与本文公开的核酸序列同源性至少99％或99.9％。

也最优选，本发明BVDV具有本发明具有本发明所述E^rns突变(密码子H300被亮氨酸密码子取代)，它由SEQ ID No.11(称作XIKE-C)的核酸序列或其功能变体编码。也最优选，本发明BVDV具有本发明E^rns突变和N^pro突变两者，其中编码组氨酸300的密码子被编码亮氨酸的密码子取代，另外还删除了除密码子1-4以外的完整N^pro编码区，因此保留了N^pro的氨基酸MELF。所述突变由SEQ ID No.12(称作XIKE-C-NdN)的核酸序列或其功能变体编码。优选，这种功能变体至少65％同源于本文公开的核酸序列。更优选，这种功能变体与本文公开的核酸序列同源性至少70％，71％，72％，73％，74％，75％，76％，77％，78％，79％，80％，81％，82％，83％，84％，85％，86％，87％，88％，89％或90％。也更优选，这种功能变体与本文公开的核酸序列同源性至少91％，92％，93％，94％，95％，96％，97％或98％。最优选，这种功能变体与本文公开的核酸序列同源性至少99％或99.9％。

本发明的另一个重要方面是使瘟病毒减毒的方法，特征在于在瘟病毒中产生糖蛋白E^rns编码序列中的至少一个突变和N^pro编码序列中的至少另一个突变。按照优选的实施方案，所述瘟病毒是BVDV。

按照更优选的实施方案，所述方法包括下列步骤：

a)将野生型瘟病毒核苷酸序列逆转录成cDNA；

b)克隆该cDNA；

c)向该cDNA中导入突变，所述突变选自删除、插入突变和/或取代突变且所述突变位于糖蛋白E^rns和蛋白酶N^pro的编码序列中，

d)将该cDNA掺入能在体外或感染合适的细胞后指导将瘟病毒cDNA转录成RNA的质粒或DNA病毒中。

本发明使BVDV减毒的方法优选包括下列步骤：

a)将野生型BVDV核苷酸序列逆转录成cDNA；

b)克隆该cDNA；

本发明另一重要实施方案是治疗瘟病毒引起的疾病的方法，其中本发明瘟病毒或本发明组合物，其中将该瘟病毒或该组合物以技术人员已知的合适剂量给予有需要的动物，并且减轻所述瘟病毒感染的症状。

本发明另一重要实施方案是治疗BVDV引起的疾病的方法，其中本发明BVDV或本发明组合物，其中将所述BVDV或所述组合物以技术人员已知的合适剂量给予有需要的动物，并且监测BVDV感染症状诸如病毒血症和白细胞减少和/或发热和/或腹泻的减轻情况。

实施例

下面的实施例用于进一步说明本发明，但不应理解为限制本文公开的发明的范围。

实施例1

BVDV XIKE-B：在怀孕母牛中评价胎儿致病性

BVDV XIKE-B是高度致病的BVDV 2型分离物NewYork′93/C的一种RNA酶阴性突变体，其从感染性cDNA克隆pKANE40B收获，并在组织培养中表现出野生型样(wt-样)生长特性。在动物实验中发现这种突变的病毒被大大减毒，因此其是一种用于开发减毒活疫苗病毒的有希望的候选者(Meyer et al.，2002)。为检测是否这种减毒病毒仍能通过胎盘并感染胎儿，用XIKE-B感染怀孕的母牛。使用从cDNA克隆pKANE40A收获的野生型BVDV作为对照。这种病毒称XIKE-A，其在感染的细胞中表达有活性的E^rns RNA酶。

本研究目的在于评价XIKE-A和XIKE-B在怀孕动物中的安全性。

实验设计

从BVDV阴性兽群(herd)选择10只怀孕的母牛(heifer)。该实验中包括下列组别，每组5只母牛：

接种前8天将母牛移至实验设施内。转运至实验设施内后确认怀孕状态。在接种当天母牛处于妊娠60到90天之间。在同一时间点对所有动物接种。

在观察期间监测母牛有无BVDV感染的临床表现，包括流产。从这些动物收集血液标本进行血清学，抗原检测，并计数白细胞。感染后9周终止实验。将未流产的母牛处死，检查并收集子宫。常规尸检收集胎儿器官标本并检测BVDV感染。

有无胎儿感染是主要评价参数，其取自实验终止时与BVDV-相关的母牛死亡数、与BVDV-相关的流产数以及BVDV阳性胎儿数。除了该主要参数，还评价BVDV感染的特征性临床表现、母牛体内的病毒血症和白细胞计数以及病毒攻击后的直肠温度。

动物

母牛购自无BVDV的农场。

只使用符合下列纳入原则的动物。

纳入原则

-无BVD抗体，在转运前和研究开始时(在动物检验设施处)，在血清抗体检验中检测每个个体。

-无BVDV，用合适的试验检测每个个体的血浆和/或血沉棕黄层制备物。

-在研究开始时体检评价临床健康。按照目前通用的兽医实践完成对这些动物的健康检查。

-在接种前通过身体检查确认怀孕。通过受精记录证实在接种时怀孕在60天至90天之间。

实验株A

说明：	XIKE A，活病毒BVDV株
		组合物：	包括低传代(low passaged)XIKE-A的细胞培养上清的实验材料
BVD成分：	BVDV II型株：XIKE-A
		提供者：	Dr.Gregor Meyers，“Bundesforschungsanstalt für Viruskrankheitender Tiere”(BFAV)，Paul-Ehrlich-Straβe 28，72076 Tübingen，Germany
BVD病毒量：	1型株：10⁵TCID₅₀/6ml(TCID＝组织培养感染剂量)
		应用的疫苗体积：	每个鼻孔3毫升
应用途径：	鼻内
		剂型的制备：	运送接种物，其为干冰上50ml小瓶中待稀释的冻干形式，将其贮存在-70℃，以备接种。在即将接种第1组母牛之前，将所述材料解冻，避免局部温度超过37℃。当肉眼看不到液体中有冰后，将材料轻轻搅动并立即用于接种动物。
未使用的接种物：	测定未使用的材料的体积，将其分成两份，在干冰或液氮中急冻，贮存，以备再次滴定。将病毒和污染的塑料或玻璃器皿与适量体积的8-10％福尔马林溶液在室温保温至少24小时，灭活病毒，之后丢弃。

实验株B

说明：	XIKE B，活病毒BVDV株
		组合物：	包括低传代XIKE-B的细胞培养上清的实验材料
BVD成分：	BVDV II型株：XIKE-B
		提供者：	Dr.Gregor Meyers，“Bundesforschungsanstalt für Viruskrankheitender Tiere”(BFAV)，Paul-Ehrlich-Straβe 28，72076 Tübingen，Germany
应用的BVD病毒剂量：	1型株：10⁵TCID₅₀/6ml(TCID＝组织培养感染剂量)
		应用的疫苗体积：	每个鼻孔3毫升
应用途径：	鼻内
		剂型的制备：	运送接种物，其为干冰上50ml小瓶中待稀释的冻干形式，将其贮存在-70℃，以备接种。在即将接种第2组母牛之前，将所述材料解冻，避免局部温度超过37℃。当肉眼看不到液体中有冰后，将材料轻轻搅动并立即用于接种动物。

未使用的接种物：

测定未使用的材料的体积，将其分成两份，在干冰或液氮中急冻，贮存，以备再次滴定。将病毒和污染的塑料或玻璃器皿与适量体积的8-10％福尔马林溶液在室温保温至少24小时，灭活病毒，之后丢弃。

妊娠控制

即将接种前确认怀孕。

母牛的接种

接种是在实验的第0天。

通过无针头的注射器对每个鼻孔内给予3毫升实验材料。每次使用新的无菌注射器。为了尽量减少呼出材料导致的液体损失，在吸气时给药。

接种后观察

血液标本的收集和检查

按照标准的无菌操作(消毒出血位点)收集血液。每个动物使用新的无菌注射器和针头。

收集血液制备血清

在即将接种前，以及在感染后每周和在研究终止时从母牛收集至少10毫升血液。将血清贮存于-20℃直至需要。

收集血液用于白细胞计数和制备血沉棕黄层(buffy coat)

对于白细胞计数而言，收集3毫升血液，之后立即转移至预先装有0.06毫升EDTA(0.235MOL/L)的合适的无菌管(Venoject，Terumo Europe N.V.，Leuven，Belgium)中。

对于制备血沉棕黄层而言，收集至少15毫升血液，之后立即转移至预先装有0.1毫升肝素溶液(注射用肝素钠，5000IU/毫升，批号A7B163A有效期至：11/2000：Gedeon Richter RT，Budapest，Hungary)的合适的无菌管中，最终在血液标本中每毫升血至少20IU的肝素。之后将内容物小心混匀。

为了制备血沉棕黄层和计数白细胞，在以下时间点从母牛收集血液

·自感染后第0天至第14天之间，每天采血；

·自第15天至第40天之间或者直至所有动物在三个连续采样时间点分离病毒结果为阴性时，每两天采血。

血清的制备

使血液在室温凝集，并离心分离。将每个血清标本分成两份，每份至少2毫升。其是一组用于ELISA测定BVDV特异性抗体。其余血清冷冻并贮存于-20℃直至需要。

白细胞计数

用标称精确度为0.1×10⁹/l，100/μl的库尔特粒度仪半自动电子装置(coulter-counter semi-automated electronic device，Diatron Minicell-16，Messtechnik GmbH，Wien，Austria)确定白细胞数目。该装置按照生产商的建议使用(校准和计数白细胞)。

血沉棕黄层的制备

尽快将肝素血液标本转运至实验室。在无菌条件下(无菌吸头，无菌操作，干净的台面等等)按照标准实验室操作进行血沉棕黄层的制备步骤。

将获得的血沉棕黄层重悬于小体积(2毫升)的RPMI 1640中并以两份0.5毫升冻于-70℃。将余下的1毫升血沉棕黄层立即用于在容许性细胞培养物中通过共培养判定与BVDV结合的血细胞。

BVD血清抗体的ELISA检测

用合适的有效ELISA试验(Svanovir^TM BVDV antibody test Cat#10-2200-10)检测每份血清标本中BVDV-抗体的存在。按照生产商的建议验证并进行试验。按log 2水平稀释阳性标本以判定BVDV抗体滴度。

BVD抗原测定

通过将新鲜制备的血沉棕黄层与易感细胞或细胞系共培养测定每份血沉棕黄层标本中BVDV的存在。共培养前不允许冷冻。

从每份标本收集血浆并提供给Man-Gene。

临床观察

母牛的观察

在接种后第0-42天由训练有素的兽医每天检查动物有无临床表现。

记录并描述所有临床表现，为其性质、坚固性(consistence)/触感、严重度(轻、中或重)、位置、影响面积的大小，按照公认的标准定义评分。特别注意呼吸道症状(呼吸，其速率；鼻或眼分泌物；结膜炎，喷嚏，咳嗽等等)和腹泻。

直肠温度

从接种前5天到感染后21天，每天在同样的小时时间(优选早晨)测定每只母牛的直肠温度。

持续每天测量直肠温度直到每只动物至少连续3天直肠温度低于或等于39℃。

检测妊娠中断

通过直肠检查确认妊娠、及对流产或胎儿的再吸收。在接种当时、接种后1和2个月由训练有素的兽医检查所有动物。按照常规兽医操作检查。

每天检查母牛的任何流产迹象直至研究终止(病毒攻击后8-12周)。

研究的终止

通过屠杀母牛并提取胎儿终止研究。将胎儿和胚胎物质转移至标有母牛号码和日期/时间的密闭的转运容器。将容器运至选定的尸检房间。

母牛不需要尸检。按下文所述对胎儿进行尸检，记录所发现的情况并收集一组标本。

死后检查

对每例死亡病历进行实验动物的详细尸检。

由有经验的外科兽医完成死后检查并在合适的数据单上记录数据。按照临床表现和观察到的损害进行进一步的实验室检查。当尸检诊断为由微生物因子引起的疾病时，通过特异于该因子的适当试验验证该诊断。

将每个组织标本收集在至少2个分别的带标记的容器中，并速冻在液氮中。将标本贮存在-70℃直至需要。

流产的胎儿和研究终止

从胎儿收集至少下列组织标本：

·腹腔或胸腔的渗出物，如果有，

·肠系膜淋巴结，

·脾，

·胸腺，

·脑(cerebellum)，

·肾，

·胸骨的骨髓，

·胎盘的标本，如果有。

死亡或处死的母牛

收集至少下列组织标本：

·用于提供血沉棕黄层的血液，如果有，

·用于提供血清的血液，如果有，

·派伊氏板(Peyer’s patches)，

·肠系膜淋巴结，

·脾，

·肾，

·子宫，包括来自胎盘的标本，如果有。

标本的贮存和运输

标本：	贮存：
		血清	-20℃
血沉棕黄层	-70℃
		病毒	-70℃
母牛的组织	-70℃
		胎儿的组织	-70℃

根据提供者的要求将标本送去进行实验室分析。标本的选择和运输的耗时由研究组领导或项目组管理者同意。一般来讲，流产材料或新生小牛的标本尽快进行研究。

结果

死亡率

第626号母牛(第1组)于PI(接种后)第13天死亡。下表总结了观察到的临床表现和尸检中揭示的损害：

母牛	生前观察	死后发现
			第626号	·自7 DPI有疾病表现·7-12 DPI流泪，流鼻涕·8-12 DPI食欲丧失·11-12 DPI腹泻·9-10和12 DPI呼吸速率加快·9 DPI咳嗽·12 DPI异常呼吸	·脱水·浆膜出血·派伊氏板充血·肺水肿

这些临床表现和肉眼观察到的病理发现与BVDV导致的损害一致，因此，可以得出结论：死亡原因是BVDV感染。

感染后流产

在每一组有一只母牛有临床流产。第615号母牛(第1组)在PI第38天流产，第469号母牛(第2组)在PI第39天流产。两者的胎儿表现出自溶现象，并且估计它们在流产前至少3-7天死亡(大约32-35DPI)。

在第1组，研究终止时处死第526号母牛经检查发现无胎儿。肉眼观察子宫病理情况有以下发现：

·子宫右角轻度扩张，

·腔内保留有进行性自溶的胎盘残余物。

第526号母牛中的发现与“沉默”流产一致，最有可能是因BVD感染。

对母牛的临床观察

各组的临床观察数据和临床表现持续时间的总结列在下面。

观察时的临床表现和接种后天数(DPI)

第1组(XIKE-A)

^*第626号母牛死于PI第13天

第2组(XIKE-B)

第1组所有被XIKE-A感染的动物表现出多种临床表现。首先出现呼吸症状伴随食欲丧失，几天后除第526号母牛外的其它母牛腹泻。感染后一只母牛死亡、另一只流产(见前面)。所有这些症状与预期的用有毒力的BVDV株感染后的症状一致。

第2组所有用XIKE-B感染的动物无临床表现。同时，在观察期间一只母牛流产。

直肠温度

这些动物感染前未检测到异常温度改变。

在第2组，感染后从第0天到第21天，所有温度值保持在生理范围内。

感染后在第7-11 PI期间检测，所有第1组动物的直肠温度升高。

研究终止时的发现

在研究终止时，处死并当场检查胎儿。

未从第526号获得胎儿(见10.2“感染后流产”章节)。

在胎儿尸检时观察到下面的发现：

这些发现表明2只第1组动物(第598和第618号母牛)和一只第2组动物(第619号母牛)在提取前几周死亡，因此认为它们是流产。

由死后发现来修正流产

死后检查后不清楚为什么某些母牛没有流产。应将死胎考虑成流产，因此在研究终止后将临床资料修改如下：

第1组

动物编号	结论
		526598615618626	BVD流产(死后子宫有胎盘)BVD流产(死后有胎儿)临床BVD流产BVD流产(死后有胎儿)死于BVD

第2组

动物编号	结论
		469565588608619	临床BVD流产预期BVD流产；无活力胎儿正常正常BVD流产(死后有胎儿)

血液标本检查

白细胞计数

因为所有动物在PI第26天病毒分离结果为阴性，所以WBC计数在该时间点中断。

将0 DPI的值认为是对比的个体基线。

在第2组中，直到观察期结束(26 DPI)白细胞计数从没达到基线值40％或更低。

在第1组中，一只动物(第598号母牛)的WBC计数有1天低于基线40％。

血清学

选择的动物在感染前其血清中均无BVDV特异抗体。

感染后，从PI 3周检测到第1组所有存活的母牛产生BVDV特异抗体，并且在所有研究动物中都持续到观察期结束。在第2组，从PI 4周检测到五只母牛4只有BVDV特异抗体。可测定的抗体应答仅在3只动物中持续到观察期结束。第2组中的滴度低于第1组的。

通过共培养检测病毒

血沉棕黄层

检测全部两组的BVDV。病毒检测的耗时总结如下。所有标本在收集后立即共培养，即不冷冻。

组织标本

在死亡的母牛和胎儿中BVD病毒的存在总结如下：

母牛：

胎儿：

NT＝未检测

标本在收集后立即共培养(即不冷冻)，带“#”标记者例外，它只有冷冻标本。

BVD相关临床和实验室数据的总结

第1组

动物编号	结论	BVD
			526598615618626	BVD流产(死后子宫有胎盘)BVD流产(死后有胎儿)临床BVD流产BVD流产(死后有胎儿)死于BVD	NT(未找到标本)+(胎儿)^-(胎儿)^-(胎儿)^*+(胎儿)/+(母牛)

NT：未检测

第2组

动物编号	结论	BVD
			469565588608619	临床BVD流产预期BVD流产；无活力胎儿正常正常BVD流产(死后有胎儿)	-(胎儿)^+(胎儿)-(胎儿)+(胎儿)-(胎儿)^

^*取样时胎儿自溶

结论：

本研究目的是评价在妊娠动物中XIKE-A和XIKE-B的安全性。

从BVDV阴性兽群选择10只怀孕的母牛。该实验中包括两组，每组5只母牛。一组接种XIKE-A病毒株，另一组接种XIKE-B病毒株。在接种当天母牛处于妊娠期60-90天之间。

结果证明，XIKE-B病毒比XIKE-A致病性低，然而在XIKE-B组也观察到与BVD相关的流产和胎儿感染。因此可以得出结论，灭活E^rns RNA酶不能防止胎儿感染。

实施例2

BVDV XIKE-A-NdN：评价在怀孕母牛中的胎儿致病性

已经发现N^pro对于组织培养中CSFV的生长并非必需(Tratschin et al.，1998)。即使仍然缺少N^pro删除致使BVDV减毒的证据，但该蛋白在病毒与宿主间相互作用中似乎可能起作用，并且最近关于CSFV的实验的确显示了这点(Mayer et al.，2004；Rüggli et al.，2003)。因此我们想研究是否删除N^pro编码序列的主要部分导致病毒不再感染妊娠母牛体内的胎儿。按照标准操作从全长cDNA克隆pKANE40A中删除除5’端4个密码子外的N^pro基因。将所得的突变的全长克隆用作模板进行体外转录，并如描述的(Meyer et al.，2002)将所得的cRNA转染MDBK细胞。将回收的病毒在组织培养中扩增然后用于下面描述的动物实验。由于前面显示BVDV XIKE-B能够通过胎盘(实施例1)，所以将其用作对照。

研究目标/目的

本研究目的在于评价基因组N^pro编码区主要部分被删除后的减毒活BVDV在怀孕动物中的安全性。

材料与方法在实施例1中描述。

研究设计

将8只怀孕母牛随机分成两组。按照下面的计划对其进行处理和观察：

研究类型：	开放型控制型临床研究
		实验单位：	个体动物
盲法：	部分盲法。不提供详细的盲法步骤和对处理方案的评价。观察的兽医位于研究现场，病理学人员则不清楚该处理；他们仅了解与其任务有关的实验方法部分。由研究者或其指派者进行疫苗接种。由研究者将标本编号以备病毒分离，直至获得了所有的结果。

结果

在研究开始时所有母牛是健康的且已怀孕。经证实所有动物在开始时无BVDV也无BVDV抗体。

用于感染的病毒的制备和控制

在稀释步骤全程收集标本并于制备的当天通过在合适的组织培养物上共培养进行测定，即不冷冻。病毒滴定的结果如下表所示。

BVDV感染的临床症状

下表给出了在观察期间有临床表现的动物的总结。

临床表现以及观察到这些临床表现时所处的接种后天数(DPI)

第1组(XIKE-A NdN)

第2组(XIKE-B)

每组部分动物仅观察到轻度和短暂的临床表现。

在第1组，五只母牛中有1只在PI第8天丧失食欲。在第2组，三只动物中有2只有临床表现。两组母牛在大约PI第21天出现咳嗽，其中一只动物伴有食欲丧失。

直肠温度

这些动物在接种前未检测到异常的温度改变。仅有几例接种后测得温度升高，见下表。

组别	动物ID	温度(℃)	PI天数
				1	15831621	39.939.0	85
2	14381585	39.040.8	29

每组有一只动物温度略升，每组也有一只动物发热。发热在PI第8或9天检测到。温度值总是在第二天回到正常。

白细胞计数

所有组在PI第3-8天观察到有部分出现白细胞减少。白细胞计数降低至少40％的动物数目如下：

组别	有白细胞减少的动物数目/总数
		12	3/5(60％)1/3(33％)

血清学(BVDV抗体)

与研究方案相顺应，在接种前所有母牛无BVDV抗体。在第1组(用XIKE-A NdN接种)和第2组(用XIKE-B接种)中，只在研究终止时(接种后2个月)检测到完全的血清转化。

从血沉棕黄层分离病毒

未检测到病毒血症。

从胎儿组织标本中分离BVD病毒

	第1组	第2组
			N	5	3
处理	XIKE-A-NdN	XIKE-B/对照
			途径	肌肉内	肌肉内
检测到胎儿传播的胎儿数目：	5个感染的胎儿中的4个	3个感染的胎儿中的2个
			用于处理的病毒具有通过胎盘传播的潜力的结论	观察到XIKE-A-NdN的胎儿传播	观察到XIKE-B的胎儿传播

结论

与表现高度致病性的母系病毒XIKE-A(Meyer et al.，2002)相比，N^pro删除导致BVDV明显减毒。然而，在接种怀孕的母牛后，只删除N^pro不能防止基于NY93的病毒重组体向胎儿传播。

实施例3

BVDV XIKE-B-NdN：评价在怀孕母牛中的胎儿致病性

为能检测RNA酶失活和N^pro删除联合对于BVDV减毒和胎儿传播的潜在影响，基于pKANE40A删除密码子349后得到的RNA酶阴性突变体pKANE40B这种感染性cDNA克隆，来构建不同BVDV-2突变体，它们在N^pro编码区中包含删除。分析回收的病毒中是否存在所需突变、是否在所导入的改变区域的侧翼不存在第二个位点突变，还分析这些病毒在组织培养物中的生长特性。XIKE-B-NdN(V-pK88C)作为一种变体，除了删除密码子349使RNA酶失活外，删除了除密码子1-4以外的全部N^pro编码区，由于它不仅具有所需突变还具有可接受的生长特性，因此选它进行动物实验。本研究的目的是评价活的减毒BVDV分离物在怀孕动物中的安全性。

将5只BVDV-阴性，怀孕的母牛用感染剂量10⁵TCID50/动物的XIKE-B-NdN鼻内接种(后面滴定数据描绘于表3.1)。每天记录临床数据。收集血液标本进行白细胞计数，血沉棕黄层制备和血清学。研究终止后收集胎儿组织进行病毒分离。

材料与方法：

如实施例1详述的：

结果

未观察到临床资料(数据未显示)。除了第1015号母牛有一天(PI第6天)白细胞计数比基线值(第0天)低约40％这样的明显降低以外，白细胞计数实际上保持不变(数据未显示)。

a)血沉棕黄层制备物的分析：

将大约10⁶白细胞(平行双份)与MDBK细胞在24-孔组织培养板中培养5天。将标本冻融两次。将100微升已解冻的标本接种在新鲜接种的24-孔组织培养板上并通过间接免疫荧光染色测试病毒(单克隆抗体Code 4，抗非结构蛋白NS3中的保守表位)。从第921，1013，1015，1055和1075号动物的血沉棕黄层制备物中未分离出BVDV(表3.2)，而阳性对照清楚表明该测试操作正确。

b)胎儿组织的死后检查

研究终止后收集下列胎儿组织进行病毒分离：脾，肾，胸腺，胸骨，脑，胎盘，小肠和腹液。简言之，用无菌海沙和经冰冷却且无Ca²⁺和Mg²⁺的PBS在研钵中制备组织悬液。用1毫升经冰冷却且无Ca²⁺和Mg²⁺的PBS冲洗研钵并将悬液于2000×g(4℃)离心10分钟。将上清先通过一次性0.45μm滤膜支架，随后第二次过滤(0.2μm孔径)。在24孔组织培养板(37℃，7％CO2)中MDBK细胞单层上进行平行双份病毒分离(400μl胎儿组织悬液或100μl胎儿腹液)。每天监控组织标本的致细胞病变效应或细菌污染，保温5天后将板冻融两次。将100μl样品传代至新鲜接种的MDBK细胞。用间接免疫荧光染色(单克隆抗体Code 4)检测病毒。在组织标本或胎儿腹液中未能检测到BVDV(表3.3)。

c)血清学发现

在接种前，接种后1个月和研究终止时判定血清中和滴度。所有动物的血清以三份平行测试抗NY93/C中和抗体，并且用间接免疫荧光染色读取终末点稀释度。结果表示为终末点稀释度，其中和大约100 TCID₅₀并用Kaerber方法计算。在感染后第0天、感染后1周和2周未获得确切数据，因为血清稀释度高达1∶16时对MDBK细胞是有毒的，在更高稀释度未能检测到中和作用。接种后第3周开始，所有动物出现抗同源的BVDV-2病毒NY’93/C的中和抗体，持续到实验结束(表3.4和图1)。

d)结论

动物研究期间获得的数据清晰显示BVDV XIKE-B-NdN代表高度减毒的病毒。与在怀孕母牛中显示高速率胎儿传播的野生型病毒或单一突变体XIKE-B或XIKE-A-NdN对比，双突变体不通过胎盘。BVDV XIKE-B-NdN以及相似的双突变体非常适合用于活的减毒疫苗。

研究编号：B01 BIVI020和B01 BIVI022

表3.1：病毒的回复滴定(Back Titration)

有效性和交叉保护研究

用减毒病毒突变体BVDV XIKE-B或BVDV XIKE-B-NdN接种必须面对两个可能的问题。首先，BVDV-1和BVDV-2之间一般有交叉保护的问题。在怀孕动物中，至少用灭活的BVDV-1疫苗接种不能预防BVDV-2向胎儿传播。因为抗胎儿感染的保护代表抗BVDV接种的主要目的，因此不能认为这种疫苗诱导广泛的保护性免疫力。因此问题是，是否用活的减毒BVDV-2接种能预防病毒向胎儿传播。第二，降低的BVDV XIKE-B-NdN生长速度会导致仅仅低水平的保护，不能预防在怀孕母牛中跨胎盘感染胎儿。为说明这些问题，开始动物研究。将动物(2组，每组10只)用BVDV XIKE-B或XIKE-B-NdN接种(预计剂量：1毫升上清有10⁵TCID₅₀的病毒；回复滴定如表3.5所示)。除未接种的对照组有一只动物轻度咳嗽一天外，这些动物接种后无一有临床表现。除了未接种的对照组有一只动物直肠温度值39.1℃一天外，其它动物的该值都低于39℃。如上述分析接种后制备的血沉棕黄层标本中病毒的存在。实验显示20只动物中仅有5只在感染后第4-8天期间有1天或2天在其血液中含有病毒(表3.6)。

表3.5：用于接种的病毒的回复滴定

接种后4周，进行动物的受精。在60-90天后用BVDV-1株(BVDV KE-9，异源攻击，动物用XIKE-B接种)或者异源BVDV-2株(BVDV KE-13，同源攻击，动物用XIKE-B-NdN免疫)进行攻击感染(预计剂量：在6毫升中的10⁵TCID50；回复滴定显示于表3.7)。从每组接种的5只怀孕的母牛中随机选取进行攻击感染。BVDV XIKE-B接种的动物用BVDV-1 KE-9株攻击，而BVDV XIKE-B/NdN接种的母牛用BVDV-2 KE-13攻击。此外，将2只未接种的对照动物用每种攻击病毒感染。

研究编号/Id.：B01 BIVI020/BVDV TüXIKE-B-NdN；胎儿保护研究

表3.7：攻击病毒的回复滴定

标本1：接种物原种(stock of inoculate)

标本2：接种物恢复稳定后的原种(stock of inoculate returned from the stable)

标本3：过量接种

^*KE9的第二次接种，标本2由于细胞死亡所以无法判断。

^**KE9，标本3由于细胞死亡或细菌污染所以无法判断。

^***KE13的第一次接种，标本3由于细菌污染所以无法判断。

接种的动物经病毒攻击感染后未表现出病毒血症或临床症状。攻击是成功的，因为所有未接种的对照都是BVDV阳性(表3.8)。在对照组仅观察到轻度的疾病表现。白细胞计数接近正常(未显示)。

在接种前，接种后1个月，攻击前，攻击后1个月和研究终止时判定血清中和滴度。所有动物的血清一式三份测试抗KE9和NY93/C(1456Nase)中和抗体，并且用间接免疫荧光染色读取终末点稀释度。结果表示为终末点稀释度，其中和大约100TCID₅₀并用Kaerber方法计算。在某些更高抗体滴度时，使用的终末点稀释度不够高。只有用XIKE-B接种的动物大约第4周开始有低滴度的抗KE9抗体。通过病毒攻击，所有动物都有抗体滴度，其在攻击后大约第4周开始显著升高。XIKE-B接种的动物比XIKE-B-NdN接种的动物具有更高的抗体滴度。在接种后4周所有动物产生大约相同中和滴度的抗NY93/C，在XIKE-B-NdN接种的动物中滴度略低。攻击后所有动物有高的抗体滴度。图2显示抗KE9(BVDV-1)的血清中和测定，图3显示抗NY93/C(BVDV-2)的血清中和测定。

分析终止研究后从胎儿获得的组织标本，结果显示，从接种的动物获得的材料得到阴性结果，而在所有4个对照动物中则发生传播(表3.9)。因此，很明显，现有的BVDV-2突变体是很适合作为有效交叉保护的疫苗病毒。

结论

由于未接种的对照有BVDV病毒血症，并且所有未接种对照的胎儿表现为BVDV阳性，所以攻击是成功的。

在目前实验和测定条件下，两种分离物都引发完全保护。根据攻击后BVD病毒血症和向胎儿的传播显示，具有单个遗传标记的分离物XIKE-B可针对1型BVDV攻击提供交叉保护。根据攻击后BVD病毒血症和向胎儿的传播，有双遗传标记的分离物XIKE-B-NdN能对抗异源的2型BVDV攻击株提供完全保护。

1.在目前实验和测定条件下，根据攻击后BVD病毒血症和向胎儿的传播，分离物XIKE-B(2型分离物)可对抗1型BVDV攻击提供交叉保护(n＝4)。

2.在目前实验和测定条件下，根据攻击后BVD病毒血症和向胎儿的传播，分离物XIKE-B-NdN(2型分离物)可对抗异源的2型BVDV攻击株提供完全保护。

实施例4

N^pro突变体的建立

进一步分析带有N^pro删除的BVDV-2突变体。构建出在基因组N^pro-编码区有删除的不同突变体。起初，只导入真性删除或伴有点突变的删除。

A：[N^pro]₁-[C-term]；

B：[N^pro]₃-[C-term]；

C：[N^pro]₄-[C-term]；

D：[N^pro]₆-[C-term]；

E：[N^pro]₄-[C-term^*]

在通式中[N^pro]_x代表保留在突变的聚蛋白氨基酸中的N^pro氨基末端残基数，[C-term]是除了N^pro外的完整聚蛋白(始于C蛋白止于NS5B)，[C-term^*]与[C-term]相同，但是在C蛋白的位点2有突变(N取代D)。

回收的病毒的生长速度远远低于野生型XIKE-A的或RNA酶阴性突变体XIKE-B的。对这一发现有两种可能的解释：(i)在不同病毒株中，N^pro-编码区不同长度的序列对有效翻译起始是必需的(Myers et al.，2001；Tautz etal.，1999)和(ii)额外序列融合到衣壳蛋白的氨基末端干扰了衣壳蛋白的功能。

为获得生长更好的N^pro删除突变体，用牛泛素基因或者牛LC3-编码序列的片段取代N^pro基因的主要部分产生了第二组突变体。这些构建物能进行有效翻译并产生带有正确氨基末端的衣壳蛋白。

[N^pro]₂₂-[PS]-[C-term]

其中PS是泛素或LC3，C-term是除了N^pro外的完整聚蛋白(始于C蛋白止于NS5B)。

这些突变体的生长速度更相似于XIKE-A的测定结果。甚至看来，通式[N^pro]₂₂-[PS]-[C-term]所示两种叫做V-pK87F和V-pK87G的RNA酶阳性病毒根本不表现出明显生长阻滞，而RNA酶阴性对应物V-pK88G在繁殖时再次有一定程度阻滞，但是比前面描述的突变体程度轻。

N^pro删除突变体的进一步的例子可以是：

MESDEGSK...

MELFSSDEGSK...

MELFSNESDEGSK...

MELFSNELSDEGSK...

MELFSNELLSDEGSK...

MELFSNELLYSDEGSK...

MELFSNELLYKSDEGSK...

MELFSNELLYKTSDEGSK...

MELFSNELLYKT代表BVDV分离物NewYork93/C的N^pro的氨基末端序列。

也可能使用此序列有一或多个突变的多种变体。特别是在其它瘟病毒中发现的天然变异预计是有功能的。因此，所测试或提议的带有N^pro氨基末端不同部分的变体的全部名单可以扩大为带有氨基酸替换的等效组群。下面给出几种瘟病毒相应序列的典型例子，但是可能的变异不局限于这些例子。

BVDV New York93/C：MELFSNELLYKT

BVDV CP13：MELISNELLYKT

BVDV SD1：MELITNELLYKT_

CSFV Brescia：MELNHFELLYKT

BDV X818：MELNKFELLYKT

因此，这些变体可以例如包括：MELI-[PS]₀-[C-term]；

MELIS-[PS]₀-[C-term]；

MELISN-[PS]₀-[C-term]；

MELISNE-[PS]₀-[C-term]；

MELISNEL-[PS]₀-[C-term]；

MELISNELL-[PS]₀-[C-term]；

MELISNELLY-[PS]₀-[C-term]；

MELISNELLYK-[PS]₀-[C-term]；

MELISNELLYKT-[PS]₀-[C-term]；

MELIT-[PS]₀-[C-term]；

MELITN-[PS]₀-[C-term]；

MELITNE-[PS]₀-[C-term]；

MELITNEL-[PS]₀-[C-term]；

MELITNELL-[PS]₀-[C-term]；

MELITNELLY-[PS]₀-[C-term]；

MELITNELLYK-[PS]₀-[C-term]；

MELITNELLYKT-[PS]₀-[C-term]；

这些通式也可以有[PS]₁，即PS也可以是本文描述的PS之一。

N^pro蛋白序列表示为斜体。BVDV NewYork93/C序列的氨基酸替换表示为粗体。

可以例如通过使用Becher et al.，2003，Virology 311，96-104)给出的GeneBank登录号，或通过标准的序列数据搜索发现其它例子。

还可以使用插入(残留的)N^pro序列和衣壳蛋白氨基末端之间的加工信号(PS)。PS导致切割，产生有功能的衣壳蛋白。这类构建物的构型可以如下：

[N^pro]₂₂-PS[C-term]

PS：加工信号。可以是蛋白酶的靶(例如本文定义的泛素，LC3或者是蛋白酶或者是导致在其自身羧基末端加工的不稳定的肽例如内含肽(Chonget al.1998和其参考文献))或者小RNA病毒的3C，心病毒或口蹄疫病毒的2A，兔出血性疾病病毒的p15或其它嵌杯样病毒的相应蛋白酶(Proter 1993，和其参考文献；Meyers et al.，2000和其参考文献)。

当使用PS时，由于PS确保产生衣壳蛋白C的正确氨基末端，所以可能有大量不同变体。因此当使用PS构建物时，只要读码框未被移位或翻译未被框内终止密码子终止，预计N^pro序列的所有类型删除或突变都导致有活力的变体。例如，我们建立了有活力的CSFV N^pro删除突变体，通式如下

[N^pro]₂₉-PS[C-term]

特别有兴趣的是N^pro突变封闭该蛋白的蛋白裂解活性。Rümenapf et al.(1998)发表的文章鉴定了CSFV Alfort Tübingen的蛋白酶的活性位点残基。相应的氨基酸(位点22的谷氨酸，位点49的组氨酸和位点69的半胱氨酸)在其它瘟病毒中是保守的。因此，将位点69的半胱氨酸替换为除了丝氨酸或苏氨酸外的任何氨基酸都导致该蛋白酶活性的破坏。相似地，改变位点22的谷氨酸很可能导致该蛋白酶的失活，除非新的氨基酸是天冬氨酸。类似地，位点49的绝大多数替换(如果不是所有替换)将导致该蛋白酶失活。

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序列表

<110>贝林格尔.英格海姆维特梅迪卡有限公司(Boehringer Ingelheim Vetmedica GmbH)

<120>含有减毒瘟病毒的疫苗

<130>Case1/1716

<160>12

<170>PatentIn version 3.1

<210>1

<211>12332

<212>DNA

<213>野生型BVDV：XIKE-A

<400>1

gtatacgaga ttagctaaag aactcgtata tggattggac gtcaacaaat ttttaattgg 60

caacgtaggg aaccttcccc tcagcgaagg ccgaaaagag gctagccatg cccttagtag 120

gactagcaaa agtaggggac tagcggtagc agtgagttcg ttggatggcc gaacccctga 180

gtacagggga gtcgtcaatg gttcgacact ccattagtcg aggagtctcg agatgccatg 240

tggacgaggg catgcccacg gcacatctta acccatgcgg gggttgcatg ggtgaaagcg 300

ctattcgtgg cgttatggac acagcctgat agggtgtagc agagacctgc tattccgcta 360

gtaaaaactc tgctgtacat ggcacatgga gttgttttca aatgaacttt tatacaaaac 420

atataaacaa aaaccagcag gcgtcgtgga acctgtttac gacgtcaacg ggcgcccact 480

gtttggagag agcagtgact tgcacccgca gtcaacacta aaactaccac accaacgagg 540

cagcgccaac atcctgacca atgctaggtc cctaccgcgg aaaggtgact gccggagagg 600

taatgtgtat ggaccggtga gtggcatcta tatcaaacca ggaccgatct actaccagga 660

ttatgtgggc cccgtctatc atagagcccc actggaacta tgtagggagg caagtatgtg 720

cgaaacaact aggagagttg gcagagtgac cggtagtgat gggaaattat atcatatcta 780

catctgcata gatgggtgta tcctcctgaa gagggcgact aggaaccaac cagaagtcct 840

gaaatgggta tacaacagat taaattgtcc tttatgggtc accagctgct ccgatgaagg 900

gagcaagggt gctacaagta agaagcagcc taagccagat aggatagaaa aaggtaagat 960

gaaaatagcc ccaaaagaga cagaaaaaga ttgcaaaacc agaccccccg acgcgactat 1020

agtagtagaa ggggttaagt accaggtgaa gaaaaaagga aaggtaaggg gaaaaaatac 1080

tcaagatggg ttatatcaca acaagaataa gccccctgaa tcaagaaaaa aattggaaaa 1140

ggcactgctg gcttgggcca tcttagcagc ggtcctgctt cagctggtaa caggagagaa 1200

tatcacccag tggaacttga tggacaacgg caccgaggga atacagcaag cgatgttcct 1260

aagaggggtg aacaggagtc tacatggaat ttggccagag aaaatttgca ccggagtacc 1320

aactcactta gcaacagact atgagcttaa agagatagtg gggatgatgg acgcgagtga 1380

gaagaccaac tacacgtgtt gcaggttgca aagacatgag tggaataaac atggttggtg 1440

taactggttt catatagaac cgtggatatg gttgatgaac aaaacccaaa acaacctgac 1500

agaagggcaa ccgcttaggg agtgtgctgt gacttgtagg tatgacaagg aaacagaatt 1560

gaacatcgtg acacaggcta gggacagacc tacaactctg acaggttgca agaaaggcaa 1620

gaatttctct ttcgcaggtg ttatactgga tgggccctgt aactttaaag tatcggttga 1680

agatgtgctg ttcaaggagc acgattgcgg caacatgctg caagagaccg cgatacagct 1740

actcgatggg gcaaccaaca ccattgaggg agcaagggta gggacggcca agttgacaac 1800

ctggttaggg aagcaattag ggatccttgg taagaagttg gagaacaaaa gcaaagcatg 1860

gtttggtgca catgcagcaa gtccatactg cggagtggag aggaagatcg gttacgtatg 1920

gtatacaaaa aactgcactc cagcttgcct tccaagaaac actagaataa taggccccgg 1980

gaaatttgat accaacgccg aagatggaaa aatactccat gagatggggg ggcacctctc 2040

agaatttgtc ctattgtcct tggtggttct gtctgacttt gccccggaaa ccgcgagcgt 2100

catctacttg gttctacatt ttgcgatccc gcaaagccac gttgatgtag acacatgcga 2160

caagaaccag ctgaatttaa cggtagcaac cacagtagca gaggtcatac cagggacagt 2220

gtggaaccta gggaagtatg tctgcataag accagactgg tggccatatg agacgacgac 2280

agtcttcgtc atagaggaag cagggcaagt aatcaaattg atgctaaggg ccatcagaga 2340

cttaactagg atatggaatg ctgccactac cacagctttc ttaatctttt tagtaaaagc 2400

actgagggga caactaatcc aagggctatt gtggctgatg ctaataacag gagcacaggg 2460

cttccctgaa tgcaaagagg gcttccaata tgccatatct aaagacagga aaatggggtt 2520

attggggcca gagagcttaa ctacaacatg gcacctcccc accaaaaaaa tagtggattc 2580

catggtgcat gtatggtgtg aaggaaaaga cttgaaaata ttaaaaatgt gcacaaagga 2640

agagaggtat ctagtggctg tgcacgagag agccttatca accagtgccg agtttatgca 2700

gatcagtgat gggacaatag gcccagacgt gatagatatg cctgatgact ttgagtttgg 2760

actctgccct tgtgactcaa aaccagtgat aaagggcaaa tttaatgcca gcttactgaa 2820

tggaccagct ttccagatgg tatgcccaca ggggtggact ggtacaatag aatgcaccct 2880

agcgaaccaa gacaccttgg acacaactgt cattaggaca tatagaagaa ctaccccatt 2940

tcagcggaga aaatggtgta cctatgaaaa aataataggg gaagatatct atgaatgcat 3000

tctaggtgga aactggacat gcataaccgg tgaccatagc aggttgaaag acggacctat 3060

caagaagtgt aagtggtgtg gccatgactt cgtcaactca gaggggctac cacactaccc 3120

aataggcaag tgcatgctca tcaacgagag tgggtacagg tatgtagatg acacctcttg 3180

cgataggggt ggtgtagcca tagttccatc tggcaccgta aagtgtagaa taggtaacgt 3240

cacggtgcaa gttatcgcta ctaacaatga tctgggaccc atgccttgca gcccagctga 3300

agtgatagca agtgaaggac cagtggaaaa gactgcatgc acattcaact attcaaggac 3360

tctacctaat aagtattatg agccaaggga ccggtacttc caacaataca tgttaaaagg 3420

ggagtggcaa tattggttcg acctggattc tgtagaccac cacaaagact acttctcaga 3480

gttcataatc atagcagtgg tcgccttgtt gggtggtaag tacgtactgt ggctcttgat 3540

aacatacaca atactgtctg agcagatggc tatgggtgct ggagtgaata ctgaagagat 3600

agtcatgata ggcaatttgc tgacagacag tgatattgag gttgtggttt atttccttct 3660

tctgtactta atagttaaag aggaactggc gaggaaatgg attatactgg tataccacat 3720

ccttgtagcc aaccctatga aaacaattgg ggtcgtctta ctaatgctag ggggagtggt 3780

gaaggccagc agaatcaatg ctgatgacca aagtgctatg gacccatgct ttcttctcgt 3840

gacaggcgta gtggctgttt tgatgatcgc tagaagagaa cctgccacat taccactgat 3900

tgtagcattg ctagcaataa gaacatcagg attcctactg cccgctagca ttgatgtaac 3960

tgtagcagta gtattaattg tacttttgtt ggctagctac ataacagact actttagata 4020

taaaaagtgg cttcaactct tatttagtct gatagctggt atctttatta taaggagctt 4080

aaaacatatc aaccagatgg aggtaccaga aatatctatg ccaagttgga gacctctagc 4140

tctggtcctt ttctatataa catctacagc aataaccact aattgggaca ttgacttagc 4200

aggcttcctg ctgcaatggg cgccagcagt gatcatgatg gctaccatgt gggcagactt 4260

tttgactctg atcatagtcc tgcccagtta cgagttatct aagctttact tcctaaagaa 4320

cgtcaggaca gacgtggaaa agaactggct cggcaaagtg aaatacagac agatcagttc 4380

agtttatgac atctgtgaca gtgaggaagc agtgtaccta tttccatcaa ggcataagag 4440

tggaagcagg ccagatttca tattaccttt tttgaaagcc gtgttaataa gctgcatcag 4500

cagccaatgg caagtggttt acatttctta cctaatactg gaaattacat actatatgca 4560

caggaaaatc atagatgagg tgtcaggagg agcaaatttt ctatcaagac tcatagcagc 4620

catcatagaa ttaaattggg ccatagatga tgaggaatgt aaaggactga agaaactgta 4680

tctcttgtca gggagagcga agaatttgat agttaaacat aaggtaagaa atgaagccgt 4740

ccacagatgg tttggtgagg aggaaatata cggggcaccc aaggtgatca ctatcataaa 4800

agctagtacc ctaagtaaaa acaggcactg cataatctgc acgatctgtg aagggaaaga 4860

atggaatgga gccaactgcc caaagtgtgg aagacaagga aagcccataa catgtggaat 4920

gacactcgca gactttgagg agaaacatta caaaaagata tttataagag aagaatcttc 4980

ttgtcctgtg ccttttgatc cttcttgcca ttgtaattat tttcgccacg atgggccttt 5040

caggaaagag tataagggtt acgtccaata cacagccaga ggacaactct ttctgaggaa 5100

cctaccaatt ctagcgacga agatgaagct attaatggtg ggaaacctcg gcgcagaaat 5160

tggcgacctg gaacatctag gatgggtact gagagggcca gccgtgtgca aaaaaattac 5220

caaccatgag aagtgccacg taaacatcat ggataagcta actgcatttt ttggaatcat 5280

gcctagaggc acgaccccta gggcacctgt gaggttcccc acagcactac taaaagtgag 5340

aagggggcta gagacgggat gggcttacac gcaccaagga gggatcagct cggtagacca 5400

tgtcacagcc ggaaaggatt tactagtgtg tgacagtatg ggcaggacca gggttgtctg 5460

tcatagtaac aataagatga ctgatgagac tgagtatggc atcaagaccg actcagggtg 5520

tcccgaaggt gcgaggtgtt acgtgctaaa cccagaagct gttaacattt ctggcacaaa 5580

aggagctatg gtacacctcc agaaaacggg gggggagttc acatgtgtca ctgcctcagg 5640

gaccccggct ttcttcgatc tgaaaaatct aaaaggctgg tccgggctac caatttttga 5700

agcatccagt ggcagggtgg ttggtagggt gaaagtcggc aagaatgagg attccaagcc 5760

caccaaacta atgagcggaa tccagacagt gtctaagaac cagacagacc tagcggacat 5820

cgtaaaaaaa ttgactagta tgaacagagg agagttcaaa cagataacat tagccactgg 5880

ggcaggaaaa actacggaac tgccaaggtc cgtcatagag gagataggga ggcacaaaag 5940

ggtcttagtc ctgataccat tgagagcagc agcagagtca gtgtatcagt atatgagagt 6000

gaagtaccca agtatatctt tcaatttgag aataggagat atgaaggaag gtgacatggc 6060

cactggtatc acctacgcct catatgggta cttttgtcag cttcctcagc ccaaactgag 6120

agctgccatg gtagagtact catatatatt cttagatgag taccactgtg ctacacccga 6180

gcaattagca ataattggaa agatacacag gtttgctgaa aatcttagag tggtagcaat 6240

gacagcaacc ccagctggaa cggtcacaac gactggtcag aaacacccta tagaggagtt 6300

catagcccca gaggtgatga aaggtgaaga tctaggtagt gaatacttgg atattgcagg 6360

gttgaagata ccgactgaag agatgaaagg caacatgctc gtgttcgcgc caactaggaa 6420

catggcagta gaaacagcta agaaattgaa ggctaaggga tacaactctg gatactatta 6480

cagtggggaa aacccagaga acttgagggt ggtaacctcg caatccccgt atgtggtagt 6540

agccaccaat gccatagagt caggtgtgac attaccagac ttagacacag ttgtagacac 6600

tggactaaag tgtgagaaga gggtgaggat ttcttcaaaa atgcccttca ttgtaacagg 6660

acttaagaga atggcagtca caatcggaga gcaagcccag cgcaggggta gagtaggaag 6720

agtcaagcca ggtaggtact ataggagtca agaaacagct tcagggtcaa aagattacca 6780

ttacgaccta ctgcaagccc agaggtacgg aatagaagat ggaattaatg taacaaagtc 6840

attcagggag atgaactatg attggagcct ttacgaagag gacagcttga tgataactca 6900

actcgaggtc cttaacaacc tccttatatc agaagacctg cctgccgcag tgaagaacat 6960

catggcccgg accgatcacc cagaacccat acaactggcc tataacagtt atgaaaacca 7020

aattccagtg ctgttcccaa agatcaaaaa tggtgaggtg acagacagtt atgagaatta 7080

cacatatctc aatgcaagaa aattaggaga ggacgtgccg gcatatgtgt acgccacaga 7140

ggatgaggat ctagcagtgg atcttctggg tatggattgg ccggacccag gcaaccaaca 7200

ggtggtagag acagggaggg cattaaaaca agtaactggc ttatccacag cagaaaacgc 7260

cctcttgata gccctattcg gctacgtcgg gtaccagaca ctttcaaaaa ggcacatacc 7320

catgattact gacatctata cacttgaaga ccacaggctt gaggacacaa cccacctcca 7380

gtttgcccca aacgctataa ggaccgacgg caaggactca gagttgaagg aattagctgt 7440

gggagacctt gataaatatg tggacgcact ggtagactac tccaaacaag ggatgaaatt 7500

catcaaagtc caagctgaaa aggtcagaga ctcccagtct acgaaggaag gcttgcaaac 7560

cattaaggag tatgtggata agtttataca atcactaaca gagaataagg aggagatcat 7620

caggtatgga ctatggggag ttcacacggc actctacaaa agcttggcag cgagactggg 7680

gcatgaaaca gcttttgcaa ctttagtggt aaaatggttg gcttttgggg gcgaaacggt 7740

atctgctcac atcaagcaag tagcagttga tctagtagta tattatatca tcaacaaacc 7800

atcttttcct ggagatacag agacccaaca agaggggagg aagtttgtgg ctagtctttt 7860

tatatctgca ctagcaacat acacatataa aacctggaat tacaacaatc tgcaacgggt 7920

tgtcgaacct gccttagctt acctcccata tgctacaagt gccttgaagt tgttcacacc 7980

cacaagatta gagagtgtgg tcatactcag ttctacaatt tacaagacat acctctctat 8040

aaggaagggt aagagtgacg gcttgttagg tacaggcata agtgcagcca tggagatctt 8100

aaaccaaaac ccaatctcag taggtatatc tgtgatgctg ggggtaggtg ccatcgccgc 8160

ccataatgca atagaatcta gtgaacagaa aagaactttg ctgatgaagg tctttgtaaa 8220

aaacttctta gaccaagcag caacagatga gctagtcaaa gagaaccctg aaaaaataat 8280

catggctcta tttgaagcag tccagaccat aggaaacccc ctaagactca tctaccatct 8340

gtacggggtg tactataagg ggtgggaagc aaaagaactc gcagagaaaa ctgctggccg 8400

caacttattc acattgatca tgtttgaggc ctttgagctt ttaggtatgg actcagaagg 8460

aaagataaga aacttgtcag gcaactacat actggactta atcttcaact tgcataataa 8520

attaaacaag gggctcaaaa aactagtcct tgggtgggct cctgcacctt tgagctgtga 8580

ttggacacca agtgatgaga gaataagcct acctcataac aactacttaa gggtagaaac 8640

caggtgtcct tgtggctatg agatgaaggc aataaaaaat gttgctggta aattgacaaa 8700

agttgaagaa aaggggtcct tcctatgcag gaatagatta gggagaggac ctccaaactt 8760

caaagtaaca aagttctatg atgataactt gatagaagtc aagccagtag ctaggctaga 8820

aggccaggtg gacctctatt acaagggagt aacagctaag ttagactaca acaatgggaa 8880

agtactgtta gctaccaaca agtgggaggt ggaccacgct ttcctgacca gactagtaaa 8940

gaagcacaca gggataggtt ttaaaggtgc atatttgggt gaccgaccag accatcaaga 9000

tcttgtcgat agagattgtg caactataac gaagaactca gtacagttcc taaaaatgaa 9060

gaagggttgc gctttcacat atgacctaac aatctctaac cttgtcaggc ttattgaact 9120

agtccataag aataatttac aagaaagaga gatccctacc gtgacagtaa ctacttggct 9180

tgcatattct tttgtcaatg aagacctggg gactatcaag cctgtattgg gggagaaagt 9240

catcccagaa ccccccgagg agttgagtct ccaacccacc gtgagactag tcaccactga 9300

aacagcaata accataacag gggaggctga agtgatgacg acagggatca caccagtggt 9360

agagatgaaa gaagaacctc agctggacca ccagtcaact accctaaagg tagggttgaa 9420

ggaaggggaa tatccagggc caggagttaa ccctaaccat ttagcagagg tgatagatga 9480

gaaagatgac aggccttttg tcctaatcat cggtaacaaa ggttctacct cgaacagagc 9540

aagaacggcc aagaatatac ggctgtacaa aggaaacaac ccaagagaga tcagggatct 9600

gatgagccaa ggaagaatat tgacggttgc tctaaaagag ttggacccgg aattaaaaga 9660

attagtagat tacaagggga cctttctcaa tagggaagct ttagaagccc taagcttagg 9720

taagccaatc aagaggaaaa ccacaacagc aatgatcagg aggttaatag agccagaggt 9780

tgaggaggaa ctaccagatt ggttccaagc ggaagaaccc ctatttttgg aagcaaaaat 9840

acagaatgac ttataccacc taattggcag tgtagatagt ataaaaagca aagcaaagga 9900

attaggggcc acagataaca caaagatagt gaaggaagtt ggggctagga cctatacgat 9960

gaaattgagc agctggagca cacaagttac aaaaaaacag atgagtctag cccctctctt 10020

tgaagagctg ttattaaagt gccctccatg tagtaaaatt tcaaagggac atatggtgtc 10080

agcataccaa ctggctcaag gaaactggga acccctcggg tgtggggtct atatgggaac 10140

cataccagct aggcgtctca agatccaccc ttatgaggct taccttaaac tcaaagagct 10200

ggtggaagtt gaatcttcga gggccactgc aaaagaatcc atcataagag aacataacac 10260

ctggatcctg cggaaggtga gacatgaagg gaacctaaga accaaatcaa tgatcaaccc 10320

tgggaaaata tcagatcagc tatgcagaga tggacacaaa agaaacatat ataataagat 10380

cataggctca acaatggcct ctgctggtat taggctggag aaactgccag tagtccgagc 10440

ccaaactgac acaaccagtt tccaccaagc cataagagaa aaaattgata aaacagaaaa 10500

caagcagacc cctgaattgc atgaagaact aatgaaggtc ttcgactgct taaagatccc 10560

agagctgaag gaatcgtatg atgaagtttc atgggaacaa ttagaagccg ggataaaccg 10620

taagggtgca gcaggctatc tagagagcaa gaacataggg gaagtcctag acacagagaa 10680

acacatagta gagcagctga tcaaggatct gaggaagggg aagaagatta ggtactatga 10740

aacagccatc cccaagaatg agaagagaga cgtcagcgac gactgggaag ccggagagtt 10800

cgttgatgaa aagaaaccaa gagtaatcca gtacccggac gccaaggtga gactggccat 10860

tacaaaagtg atgtacaaat gggtaaagca aaaaccagtg gtgatacccg gctatgaagg 10920

taaaacacct ctatttgaca tattcaacaa agtgaagaag gaatgggatt cattccagga 10980

ccccgtagca gtgagctttg acaccaaagc gtgggataca caagtcacca gtagagacct 11040

aatgttgata aaggatatcc agaaatatta tttcaagaga agtatacaca aatttttaga 11100

tacaataaca gaacacatgg tggaggtacc tgtcattaca gcagacggtg aagtttacat 11160

aaggaatggt cagaggggta gtggccaacc cgacacaagt gctggtaata gtatgttgaa 11220

tgtcctaacc atgatatatg ctttctgtaa aagtacaggc ataccttaca ggggattcag 11280

cagagtggca agaatccatg tgtgtggtga tgatggcttt ttgataacag agagaggact 11340

gggactgaaa ttctctgaga agggtatgca gatattacat gaggccggga agccccagaa 11400

aataactgaa ggggacaaaa tgaaagtggc atacagattc gaggacatag agttttgttc 11460

ccatactccc gtgccagtca gatgggcaga taacaccagt agttacatgg cagggaggag 11520

cacagccact atactagcta agatggcaac caggctggat tccagcggag agaggggtag 11580

cacagcttat gagaaggccg tagccttcag cttccttttg atgtactcat ggaatcccgt 11640

agttagaagg atctgcttac tggtgttgtc acagtttcca gaaatatccc catccaaaaa 11700

cacaatatac tactaccaag gggatcccat agctgcgtac agagaagtga tagggaaaca 11760

gctgtgtgaa ctgaaaagaa caggatttga gaagctggct ggtctgaatt tgagtatgac 11820

cactctaggc atctggacaa aacatactag taaaagacta atccaagcct gtgtagaaat 11880

aggtaagaga gaaggtacct ggttagttaa tgctgacaga ctgattgcag gaaagactgg 11940

gaagttttac atcccaagca ctggtgtcac tctgttggga aaacactatg aggaaattaa 12000

cttaaagcaa aaggcggcac aaccgccgat agagggggtt gacagatata agttgggccc 12060

catagttaat gttatcttga gaaggctgag ggtgatgctg atgacagttg ccagcggaag 12120

ctggtgaatc cgtccggagc gtcgtgccct cactcaaggt ttttaattgt aaatattgta 12180

aatagacagc taagatattt attgtagttg gatagtaatg cagtgatagt aaatacccca 12240

atttaacact acctccaatg cactaagcac tttagctgtg tgaggttaac tcgacgtcca 12300

cggttggact agggaagacc tctaacagcc cc 12332

<210>2

<211>11840

<212>DNA

<213>人工序列：突变的BVDV：XIKE-A-NdN

<400>2

gtatacgaga ttagctaaag aactcgtata tggattggac gtcaacaaat ttttaattgg 60

caacgtaggg aaccttcccc tcagcgaagg ccgaaaagag gctagccatg cccttagtag 120

gactagcaaa agtaggggac tagcggtagc agtgagttcg ttggatggcc gaacccctga 180

gtacagggga gtcgtcaatg gttcgacact ccattagtcg aggagtctcg agatgccatg 240

tggacgaggg catgcccacg gcacatctta acccatgcgg gggttgcatg ggtgaaagcg 300

ctattcgtgg cgttatggac acagcctgat agggtgtagc agagacctgc tattccgcta 360

gtaaaaactc tgctgtacat ggcacatgga gttgttttcc gatgaaggga gcaagggtgc 420

tacaagtaag aagcagccta agccagatag gatagaaaaa ggtaagatga aaatagcccc 480

aaaagagaca gaaaaagatt gcaaaaccag accccccgac gcgactatag tagtagaagg 540

ggttaagtac caggtgaaga aaaaaggaaa ggtaagggga aaaaatactc aagatgggtt 600

atatcacaac aagaataagc cccctgaatc aagaaaaaaa ttggaaaagg cactgctggc 660

ttgggccatc ttagcagcgg tcctgcttca gctggtaaca ggagagaata tcacccagtg 720

gaacttgatg gacaacggca ccgagggaat acagcaagcg atgttcctaa gaggggtgaa 780

caggagtcta catggaattt ggccagagaa aatttgcacc ggagtaccaa ctcacttagc 840

aacagactat gagcttaaag agatagtggg gatgatggac gcgagtgaga agaccaacta 900

cacgtgttgc aggttgcaaa gacatgagtg gaataaacat ggttggtgta actggtttca 960

tatagaaccg tggatatggt tgatgaacaa aacccaaaac aacctgacag aagggcaacc 1020

gcttagggag tgtgctgtga cttgtaggta tgacaaggaa acagaattga acatcgtgac 1080

acaggctagg gacagaccta caactctgac aggttgcaag aaaggcaaga atttctcttt 1140

cgcaggtgtt atactggatg ggccctgtaa ctttaaagta tcggttgaag atgtgctgtt 1200

caaggagcac gattgcggca acatgctgca agagaccgcg atacagctac tcgatggggc 1260

aaccaacacc attgagggag caagggtagg gacggccaag ttgacaacct ggttagggaa 1320

gcaattaggg atccttggta agaagttgga gaacaaaagc aaagcatggt ttggtgcaca 1380

tgcagcaagt ccatactgcg gagtggagag gaagatcggt tacgtatggt atacaaaaaa 1440

ctgcactcca gcttgccttc caagaaacac tagaataata ggccccggga aatttgatac 1500

caacgccgaa gatggaaaaa tactccatga gatggggggg cacctctcag aatttgtcct 1560

attgtccttg gtggttctgt ctgactttgc cccggaaacc gcgagcgtca tctacttggt 1620

tctacatttt gcgatcccgc aaagccacgt tgatgtagac acatgcgaca agaaccagct 1680

gaatttaacg gtagcaacca cagtagcaga ggtcatacca gggacagtgt ggaacctagg 1740

gaagtatgtc tgcataagac cagactggtg gccatatgag acgacgacag tcttcgtcat 1800

agaggaagca gggcaagtaa tcaaattgat gctaagggcc atcagagact taactaggat 1860

atggaatgct gccactacca cagctttctt aatcttttta gtaaaagcac tgaggggaca 1920

actaatccaa gggctattgt ggctgatgct aataacagga gcacagggct tccctgaatg 1980

caaagagggc ttccaatatg ccatatctaa agacaggaaa atggggttat tggggccaga 2040

gagcttaact acaacatggc acctccccac caaaaaaata gtggattcca tggtgcatgt 2100

atggtgtgaa ggaaaagact tgaaaatatt aaaaatgtgc acaaaggaag agaggtatct 2160

agtggctgtg cacgagagag ccttatcaac cagtgccgag tttatgcaga tcagtgatgg 2220

gacaataggc ccagacgtga tagatatgcc tgatgacttt gagtttggac tctgcccttg 2280

tgactcaaaa ccagtgataa agggcaaatt taatgccagc ttactgaatg gaccagcttt 2340

ccagatggta tgcccacagg ggtggactgg tacaatagaa tgcaccctag cgaaccaaga 2400

caccttggac acaactgtca ttaggacata tagaagaact accccatttc agcggagaaa 2460

atggtgtacc tatgaaaaaa taatagggga agatatctat gaatgcattc taggtggaaa 2520

ctggacatgc ataaccggtg accatagcag gttgaaagac ggacctatca agaagtgtaa 2580

gtggtgtggc catgacttcg tcaactcaga ggggctacca cactacccaa taggcaagtg 2640

catgctcatc aacgagagtg ggtacaggta tgtagatgac acctcttgcg ataggggtgg 2700

tgtagccata gttccatctg gcaccgtaaa gtgtagaata ggtaacgtca cggtgcaagt 2760

tatcgctact aacaatgatc tgggacccat gccttgcagc ccagctgaag tgatagcaag 2820

tgaaggacca gtggaaaaga ctgcatgcac attcaactat tcaaggactc tacctaataa 2880

gtattatgag ccaagggacc ggtacttcca acaatacatg ttaaaagggg agtggcaata 2940

ttggttcgac ctggattctg tagaccacca caaagactac ttctcagagt tcataatcat 3000

agcagtggtc gccttgttgg gtggtaagta cgtactgtgg ctcttgataa catacacaat 3060

actgtctgag cagatggcta tgggtgctgg agtgaatact gaagagatag tcatgatagg 3120

caatttgctg acagacagtg atattgaggt tgtggtttat ttccttcttc tgtacttaat 3180

agttaaagag gaactggcga ggaaatggat tatactggta taccacatcc ttgtagccaa 3240

ccctatgaaa acaattgggg tcgtcttact aatgctaggg ggagtggtga aggccagcag 3300

aatcaatgct gatgaccaaa gtgctatgga cccatgcttt cttctcgtga caggcgtagt 3360

ggctgttttg atgatcgcta gaagagaacc tgccacatta ccactgattg tagcattgct 3420

agcaataaga acatcaggat tcctactgcc cgctagcatt gatgtaactg tagcagtagt 3480

attaattgta cttttgttgg ctagctacat aacagactac tttagatata aaaagtggct 3540

tcaactctta tttagtctga tagctggtat ctttattata aggagcttaa aacatatcaa 3600

ccagatggag gtaccagaaa tatctatgcc aagttggaga cctctagctc tggtcctttt 3660

ctatataaca tctacagcaa taaccactaa ttgggacatt gacttagcag gcttcctgct 3720

gcaatgggcg ccagcagtga tcatgatggc taccatgtgg gcagactttt tgactctgat 3780

catagtcctg cccagttacg agttatctaa gctttacttc ctaaagaacg tcaggacaga 3840

cgtggaaaag aactggctcg gcaaagtgaa atacagacag atcagttcag tttatgacat 3900

ctgtgacagt gaggaagcag tgtacctatt tccatcaagg cataagagtg gaagcaggcc 3960

agatttcata ttaccttttt tgaaagccgt gttaataagc tgcatcagca gccaatggca 4020

agtggtttac atttcttacc taatactgga aattacatac tatatgcaca ggaaaatcat 4080

agatgaggtg tcaggaggag caaattttct atcaagactc atagcagcca tcatagaatt 4140

aaattgggcc atagatgatg aggaatgtaa aggactgaag aaactgtatc tcttgtcagg 4200

gagagcgaag aatttgatag ttaaacataa ggtaagaaat gaagccgtcc acagatggtt 4260

tggtgaggag gaaatatacg gggcacccaa ggtgatcact atcataaaag ctagtaccct 4320

aagtaaaaac aggcactgca taatctgcac gatctgtgaa gggaaagaat ggaatggagc 4380

caactgccca aagtgtggaa gacaaggaaa gcccataaca tgtggaatga cactcgcaga 4440

ctttgaggag aaacattaca aaaagatatt tataagagaa gaatcttctt gtcctgtgcc 4500

ttttgatcct tcttgccatt gtaattattt tcgccacgat gggcctttca ggaaagagta 4560

taagggttac gtccaataca cagccagagg acaactcttt ctgaggaacc taccaattct 4620

agcgacgaag atgaagctat taatggtggg aaacctcggc gcagaaattg gcgacctgga 4680

acatctagga tgggtactga gagggccagc cgtgtgcaaa aaaattacca accatgagaa 4740

gtgccacgta aacatcatgg ataagctaac tgcatttttt ggaatcatgc ctagaggcac 4800

gacccctagg gcacctgtga ggttccccac agcactacta aaagtgagaa gggggctaga 4860

gacgggatgg gcttacacgc accaaggagg gatcagctcg gtagaccatg tcacagccgg 4920

aaaggattta ctagtgtgtg acagtatggg caggaccagg gttgtctgtc atagtaacaa 4980

taagatgact gatgagactg agtatggcat caagaccgac tcagggtgtc ccgaaggtgc 5040

gaggtgttac gtgctaaacc cagaagctgt taacatttct ggcacaaaag gagctatggt 5100

acacctccag aaaacggggg gggagttcac atgtgtcact gcctcaggga ccccggcttt 5160

cttcgatctg aaaaatctaa aaggctggtc cgggctacca atttttgaag catccagtgg 5220

cagggtggtt ggtagggtga aagtcggcaa gaatgaggat tccaagccca ccaaactaat 5280

gagcggaatc cagacagtgt ctaagaacca gacagaccta gcggacatcg taaaaaaatt 5340

gactagtatg aacagaggag agttcaaaca gataacatta gccactgggg caggaaaaac 5400

tacggaactg ccaaggtccg tcatagagga gatagggagg cacaaaaggg tcttagtcct 5460

gataccattg agagcagcag cagagtcagt gtatcagtat atgagagtga agtacccaag 5520

tatatctttc aatttgagaa taggagatat gaaggaaggt gacatggcca ctggtatcac 5580

ctacgcctca tatgggtact tttgtcagct tcctcagccc aaactgagag ctgccatggt 5640

agagtactca tatatattct tagatgagta ccactgtgct acacccgagc aattagcaat 5700

aattggaaag atacacaggt ttgctgaaaa tcttagagtg gtagcaatga cagcaacccc 5760

agctggaacg gtcacaacga ctggtcagaa acaccctata gaggagttca tagccccaga 5820

ggtgatgaaa ggtgaagatc taggtagtga atacttggat attgcagggt tgaagatacc 5880

gactgaagag atgaaaggca acatgctcgt gttcgcgcca actaggaaca tggcagtaga 5940

aacagctaag aaattgaagg ctaagggata caactctgga tactattaca gtggggaaaa 6000

cccagagaac ttgagggtgg taacctcgca atccccgtat gtggtagtag ccaccaatgc 6060

catagagtca ggtgtgacat taccagactt agacacagtt gtagacactg gactaaagtg 6120

tgagaagagg gtgaggattt cttcaaaaat gcccttcatt gtaacaggac ttaagagaat 6180

ggcagtcaca atcggagagc aagcccagcg caggggtaga gtaggaagag tcaagccagg 6240

taggtactat aggagtcaag aaacagcttc agggtcaaaa gattaccatt acgacctact 6300

gcaagcccag aggtacggaa tagaagatgg aattaatgta acaaagtcat tcagggagat 6360

gaactatgat tggagccttt acgaagagga cagcttgatg ataactcaac tcgaggtcct 6420

taacaacctc cttatatcag aagacctgcc tgccgcagtg aagaacatca tggcccggac 6480

cgatcaccca gaacccatac aactggccta taacagttat gaaaaccaaa ttccagtgct 6540

gttcccaaag atcaaaaatg gtgaggtgac agacagttat gagaattaca catatctcaa 6600

tgcaagaaaa ttaggagagg acgtgccggc atatgtgtac gccacagagg atgaggatct 6660

agcagtggat cttctgggta tggattggcc ggacccaggc aaccaacagg tggtagagac 6720

agggagggca ttaaaacaag taactggctt atccacagca gaaaacgccc tcttgatagc 6780

cctattcggc tacgtcgggt accagacact ttcaaaaagg cacataccca tgattactga 6840

catctataca cttgaagacc acaggcttga ggacacaacc cacctccagt ttgccccaaa 6900

cgctataagg accgacggca aggactcaga gttgaaggaa ttagctgtgg gagaccttga 6960

taaatatgtg gacgcactgg tagactactc caaacaaggg atgaaattca tcaaagtcca 7020

agctgaaaag gtcagagact cccagtctac gaaggaaggc ttgcaaacca ttaaggagta 7080

tgtggataag tttatacaat cactaacaga gaataaggag gagatcatca ggtatggact 7140

atggggagtt cacacggcac tctacaaaag cttggcagcg agactggggc atgaaacagc 7200

ttttgcaact ttagtggtaa aatggttggc ttttgggggc gaaacggtat ctgctcacat 7260

caagcaagta gcagttgatc tagtagtata ttatatcatc aacaaaccat cttttcctgg 7320

agatacagag acccaacaag aggggaggaa gtttgtggct agtcttttta tatctgcact 7380

agcaacatac acatataaaa cctggaatta caacaatctg caacgggttg tcgaacctgc 7440

cttagcttac ctcccatatg ctacaagtgc cttgaagttg ttcacaccca caagattaga 7500

gagtgtggtc atactcagtt ctacaattta caagacatac ctctctataa ggaagggtaa 7560

gagtgacggc ttgttaggta caggcataag tgcagccatg gagatcttaa accaaaaccc 7620

aatctcagta ggtatatctg tgatgctggg ggtaggtgcc atcgccgccc ataatgcaat 7680

agaatctagt gaacagaaaa gaactttgct gatgaaggtc tttgtaaaaa acttcttaga 7740

ccaagcagca acagatgagc tagtcaaaga gaaccctgaa aaaataatca tggctctatt 7800

tgaagcagtc cagaccatag gaaaccccct aagactcatc taccatctgt acggggtgta 7860

ctataagggg tgggaagcaa aagaactcgc agagaaaact gctggccgca acttattcac 7920

attgatcatg tttgaggcct ttgagctttt aggtatggac tcagaaggaa agataagaaa 7980

cttgtcaggc aactacatac tggacttaat cttcaacttg cataataaat taaacaaggg 8040

gctcaaaaaa ctagtccttg ggtgggctcc tgcacctttg agctgtgatt ggacaccaag 8100

tgatgagaga ataagcctac ctcataacaa ctacttaagg gtagaaacca ggtgtccttg 8160

tggctatgag atgaaggcaa taaaaaatgt tgctggtaaa ttgacaaaag ttgaagaaaa 8220

ggggtccttc ctatgcagga atagattagg gagaggacct ccaaacttca aagtaacaaa 8280

gttctatgat gataacttga tagaagtcaa gccagtagct aggctagaag gccaggtgga 8340

cctctattac aagggagtaa cagctaagtt agactacaac aatgggaaag tactgttagc 8400

taccaacaag tgggaggtgg accacgcttt cctgaccaga ctagtaaaga agcacacagg 8460

gataggtttt aaaggtgcat atttgggtga ccgaccagac catcaagatc ttgtcgatag 8520

agattgtgca actataacga agaactcagt acagttccta aaaatgaaga agggttgcgc 8580

tttcacatat gacctaacaa tctctaacct tgtcaggctt attgaactag tccataagaa 8640

taatttacaa gaaagagaga tccctaccgt gacagtaact acttggcttg catattcttt 8700

tgtcaatgaa gacctgggga ctatcaagcc tgtattgggg gagaaagtca tcccagaacc 8760

ccccgaggag ttgagtctcc aacccaccgt gagactagtc accactgaaa cagcaataac 8820

cataacaggg gaggctgaag tgatgacgac agggatcaca ccagtggtag agatgaaaga 8880

agaacctcag ctggaccacc agtcaactac cctaaaggta gggttgaagg aaggggaata 8940

tccagggcca ggagttaacc ctaaccattt agcagaggtg atagatgaga aagatgacag 9000

gccttttgtc ctaatcatcg gtaacaaagg ttctacctcg aacagagcaa gaacggccaa 9060

gaatatacgg ctgtacaaag gaaacaaccc aagagagatc agggatctga tgagccaagg 9120

aagaatattg acggttgctc taaaagagtt ggacccggaa ttaaaagaat tagtagatta 9180

caaggggacc tttctcaata gggaagcttt agaagcccta agcttaggta agccaatcaa 9240

gaggaaaacc acaacagcaa tgatcaggag gttaatagag ccagaggttg aggaggaact 9300

accagattgg ttccaagcgg aagaacccct atttttggaa gcaaaaatac agaatgactt 9360

ataccaccta attggcagtg tagatagtat aaaaagcaaa gcaaaggaat taggggccac 9420

agataacaca aagatagtga aggaagttgg ggctaggacc tatacgatga aattgagcag 9480

ctggagcaca caagttacaa aaaaacagat gagtctagcc cctctctttg aagagctgtt 9540

attaaagtgc cctccatgta gtaaaatttc aaagggacat atggtgtcag cataccaact 9600

ggctcaagga aactgggaac ccctcgggtg tggggtctat atgggaacca taccagctag 9660

gcgtctcaag atccaccctt atgaggctta ccttaaactc aaagagctgg tggaagttga 9720

atcttcgagg gccactgcaa aagaatccat cataagagaa cataacacct ggatcctgcg 9780

gaaggtgaga catgaaggga acctaagaac caaatcaatg atcaaccctg ggaaaatatc 9840

agatcagcta tgcagagatg gacacaaaag aaacatatat aataagatca taggctcaac 9900

aatggcctct gctggtatta ggctggagaa actgccagta gtccgagccc aaactgacac 9960

aaccagtttc caccaagcca taagagaaaa aattgataaa acagaaaaca agcagacccc 10020

tgaattgcat gaagaactaa tgaaggtctt cgactgctta aagatcccag agctgaagga 10080

atcgtatgat gaagtttcat gggaacaatt agaagccggg ataaaccgta agggtgcagc 10140

aggctatcta gagagcaaga acatagggga agtcctagac acagagaaac acatagtaga 10200

gcagctgatc aaggatctga ggaaggggaa gaagattagg tactatgaaa cagccatccc 10260

caagaatgag aagagagacg tcagcgacga ctgggaagcc ggagagttcg ttgatgaaaa 10320

gaaaccaaga gtaatccagt acccggacgc caaggtgaga ctggccatta caaaagtgat 10380

gtacaaatgg gtaaagcaaa aaccagtggt gatacccggc tatgaaggta aaacacctct 10440

atttgacata ttcaacaaag tgaagaagga atgggattca ttccaggacc ccgtagcagt 10500

gagctttgac accaaagcgt gggatacaca agtcaccagt agagacctaa tgttgataaa 10560

ggatatccag aaatattatt tcaagagaag tatacacaaa tttttagata caataacaga 10620

acacatggtg gaggtacctg tcattacagc agacggtgaa gtttacataa ggaatggtca 10680

gaggggtagt ggccaacccg acacaagtgc tggtaatagt atgttgaatg tcctaaccat 10740

gatatatgct ttctgtaaaa gtacaggcat accttacagg ggattcagca gagtggcaag 10800

aatccatgtg tgtggtgatg atggcttttt gataacagag agaggactgg gactgaaatt 10860

ctctgagaag ggtatgcaga tattacatga ggccgggaag ccccagaaaa taactgaagg 10920

ggacaaaatg aaagtggcat acagattcga ggacatagag ttttgttccc atactcccgt 10980

gccagtcaga tgggcagata acaccagtag ttacatggca gggaggagca cagccactat 11040

actagctaag atggcaacca ggctggattc cagcggagag aggggtagca cagcttatga 11100

gaaggccgta gccttcagct tccttttgat gtactcatgg aatcccgtag ttagaaggat 11160

ctgcttactg gtgttgtcac agtttccaga aatatcccca tccaaaaaca caatatacta 11220

ctaccaaggg gatcccatag ctgcgtacag agaagtgata gggaaacagc tgtgtgaact 11280

gaaaagaaca ggatttgaga agctggctgg tctgaatttg agtatgacca ctctaggcat 11340

ctggacaaaa catactagta aaagactaat ccaagcctgt gtagaaatag gtaagagaga 11400

aggtacctgg ttagttaatg ctgacagact gattgcagga aagactggga agttttacat 11460

cccaagcact ggtgtcactc tgttgggaaa acactatgag gaaattaact taaagcaaaa 11520

ggcggcacaa ccgccgatag agggggttga cagatataag ttgggcccca tagttaatgt 11580

tatcttgaga aggctgaggg tgatgctgat gacagttgcc agcggaagct ggtgaatccg 11640

tccggagcgt cgtgccctca ctcaaggttt ttaattgtaa atattgtaaa tagacagcta 11700

agatatttat tgtagttgga tagtaatgca gtgatagtaa ataccccaat ttaacactac 11760

ctccaatgca ctaagcactt tagctgtgtg aggttaactc gacgtccacg gttggactag 11820

ggaagacctc taacagcccc 11840

<210>3

<211>12329

<212>DNA

<213>人工序列：突变的BVDV：XIKE-B

<400>3

gtatacgaga ttagctaaag aactcgtata tggattggac gtcaacaaat ttttaattgg 60

caacgtaggg aaccttcccc tcagcgaagg ccgaaaagag gctagccatg cccttagtag 120

gactagcaaa agtaggggac tagcggtagc agtgagttcg ttggatggcc gaacccctga 180

gtacagggga gtcgtcaatg gttcgacact ccattagtcg aggagtctcg agatgccatg 240

tggacgaggg catgcccacg gcacatctta acccatgcgg gggttgcatg ggtgaaagcg 300

ctattcgtgg cgttatggac acagcctgat agggtgtagc agagacctgc tattccgcta 360

gtaaaaactc tgctgtacat ggcacatgga gttgttttca aatgaacttt tatacaaaac 420

atataaacaa aaaccagcag gcgtcgtgga acctgtttac gacgtcaacg ggcgcccact 480

gtttggagag agcagtgact tgcacccgca gtcaacacta aaactaccac accaacgagg 540

cagcgccaac atcctgacca atgctaggtc cctaccgcgg aaaggtgact gccggagagg 600

taatgtgtat ggaccggtga gtggcatcta tatcaaacca ggaccgatct actaccagga 660

ttatgtgggc cccgtctatc atagagcccc actggaacta tgtagggagg caagtatgtg 720

cgaaacaact aggagagttg gcagagtgac cggtagtgat gggaaattat atcatatcta 780

catctgcata gatgggtgta tcctcctgaa gagggcgact aggaaccaac cagaagtcct 840

gaaatgggta tacaacagat taaattgtcc tttatgggtc accagctgct ccgatgaagg 900

gagcaagggt gctacaagta agaagcagcc taagccagat aggatagaaa aaggtaagat 960

gaaaatagcc ccaaaagaga cagaaaaaga ttgcaaaacc agaccccccg acgcgactat 1020

agtagtagaa ggggttaagt accaggtgaa gaaaaaagga aaggtaaggg gaaaaaatac 1080

tcaagatggg ttatatcaca acaagaataa gccccctgaa tcaagaaaaa aattggaaaa 1140

ggcactgctg gcttgggcca tcttagcagc ggtcctgctt cagctggtaa caggagagaa 1200

tatcacccag tggaacttga tggacaacgg caccgaggga atacagcaag cgatgttcct 1260

aagaggggtg aacaggagtc tacatggaat ttggccagag aaaatttgca ccggagtacc 1320

aactcactta gcaacagact atgagcttaa agagatagtg gggatgatgg acgcgagtga 1380

gaagaccaac tacacgtgtt gcaggttgca aagacatgag tggaataaag gttggtgtaa 1440

ctggtttcat atagaaccgt ggatatggtt gatgaacaaa acccaaaaca acctgacaga 1500

agggcaaccg cttagggagt gtgctgtgac ttgtaggtat gacaaggaaa cagaattgaa 1560

catcgtgaca caggctaggg acagacctac aactctgaca ggttgcaaga aaggcaagaa 1620

tttctctttc gcaggtgtta tactggatgg gccctgtaac tttaaagtat cggttgaaga 1680

tgtgctgttc aaggagcacg attgcggcaa catgctgcaa gagaccgcga tacagctact 1740

cgatggggca accaacacca ttgagggagc aagggtaggg acggccaagt tgacaacctg 1800

gttagggaag caattaggga tccttggtaa gaagttggag aacaaaagca aagcatggtt 1860

tggtgcacat gcagcaagtc catactgcgg agtggagagg aagatcggtt acgtatggta 1920

tacaaaaaac tgcactccag cttgccttcc aagaaacact agaataatag gccccgggaa 1980

atttgatacc aacgccgaag atggaaaaat actccatgag atgggggggc acctctcaga 2040

atttgtccta ttgtccttgg tggttctgtc tgactttgcc ccggaaaccg cgagcgtcat 2100

ctacttggtt ctacattttg cgatcccgca aagccacgtt gatgtagaca catgcgacaa 2160

gaaccagctg aatttaacgg tagcaaccac agtagcagag gtcataccag ggacagtgtg 2220

gaacctaggg aagtatgtct gcataagacc agactggtgg ccatatgaga cgacgacagt 2280

cttcgtcata gaggaagcag ggcaagtaat caaattgatg ctaagggcca tcagagactt 2340

aactaggata tggaatgctg ccactaccac agctttctta atctttttag taaaagcact 2400

gaggggacaa ctaatccaag ggctattgtg gctgatgcta ataacaggag cacagggctt 2460

ccctgaatgc aaagagggct tccaatatgc catatctaaa gacaggaaaa tggggttatt 2520

ggggccagag agcttaacta caacatggca cctccccacc aaaaaaatag tggattccat 2580

ggtgcatgta tggtgtgaag gaaaagactt gaaaatatta aaaatgtgca caaaggaaga 2640

gaggtatcta gtggctgtgc acgagagagc cttatcaacc agtgccgagt ttatgcagat 2700

cagtgatggg acaataggcc cagacgtgat agatatgcct gatgactttg agtttggact 2760

ctgcccttgt gactcaaaac cagtgataaa gggcaaattt aatgccagct tactgaatgg 2820

accagctttc cagatggtat gcccacaggg gtggactggt acaatagaat gcaccctagc 2880

gaaccaagac accttggaca caactgtcat taggacatat agaagaacta ccccatttca 2940

gcggagaaaa tggtgtacct atgaaaaaat aataggggaa gatatctatg aatgcattct 3000

aggtggaaac tggacatgca taaccggtga ccatagcagg ttgaaagacg gacctatcaa 3060

gaagtgtaag tggtgtggcc atgacttcgt caactcagag gggctaccac actacccaat 3120

aggcaagtgc atgctcatca acgagagtgg gtacaggtat gtagatgaca cctcttgcga 3180

taggggtggt gtagccatag ttccatctgg caccgtaaag tgtagaatag gtaacgtcac 3240

ggtgcaagtt atcgctacta acaatgatct gggacccatg ccttgcagcc cagctgaagt 3300

gatagcaagt gaaggaccag tggaaaagac tgcatgcaca ttcaactatt caaggactct 3360

acctaataag tattatgagc caagggaccg gtacttccaa caatacatgt taaaagggga 3420

gtggcaatat tggttcgacc tggattctgt agaccaccac aaagactact tctcagagtt 3480

cataatcata gcagtggtcg ccttgttggg tggtaagtac gtactgtggc tcttgataac 3540

atacacaata ctgtctgagc agatggctat gggtgctgga gtgaatactg aagagatagt 3600

catgataggc aatttgctga cagacagtga tattgaggtt gtggtttatt tccttcttct 3660

gtacttaata gttaaagagg aactggcgag gaaatggatt atactggtat accacatcct 3720

tgtagccaac cctatgaaaa caattggggt cgtcttacta atgctagggg gagtggtgaa 3780

ggccagcaga atcaatgctg atgaccaaag tgctatggac ccatgctttc ttctcgtgac 3840

aggcgtagtg gctgttttga tgatcgctag aagagaacct gccacattac cactgattgt 3900

agcattgcta gcaataagaa catcaggatt cctactgccc gctagcattg atgtaactgt 3960

agcagtagta ttaattgtac ttttgttggc tagctacata acagactact ttagatataa 4020

aaagtggctt caactcttat ttagtctgat agctggtatc tttattataa ggagcttaaa 4080

acatatcaac cagatggagg taccagaaat atctatgcca agttggagac ctctagctct 4140

ggtccttttc tatataacat ctacagcaat aaccactaat tgggacattg acttagcagg 4200

cttcctgctg caatgggcgc cagcagtgat catgatggct accatgtggg cagacttttt 4260

gactctgatc atagtcctgc ccagttacga gttatctaag ctttacttcc taaagaacgt 4320

caggacagac gtggaaaaga actggctcgg caaagtgaaa tacagacaga tcagttcagt 4380

ttatgacatc tgtgacagtg aggaagcagt gtacctattt ccatcaaggc ataagagtgg 4440

aagcaggcca gatttcatat tacctttttt gaaagccgtg ttaataagct gcatcagcag 4500

ccaatggcaa gtggtttaca tttcttacct aatactggaa attacatact atatgcacag 4560

gaaaatcata gatgaggtgt caggaggagc aaattttcta tcaagactca tagcagccat 4620

catagaatta aattgggcca tagatgatga ggaatgtaaa ggactgaaga aactgtatct 4680

cttgtcaggg agagcgaaga atttgatagt taaacataag gtaagaaatg aagccgtcca 4740

cagatggttt ggtgaggagg aaatatacgg ggcacccaag gtgatcacta tcataaaagc 4800

tagtacccta agtaaaaaca ggcactgcat aatctgcacg atctgtgaag ggaaagaatg 4860

gaatggagcc aactgcccaa agtgtggaag acaaggaaag cccataacat gtggaatgac 4920

actcgcagac tttgaggaga aacattacaa aaagatattt ataagagaag aatcttcttg 4980

tcctgtgcct tttgatcctt cttgccattg taattatttt cgccacgatg ggcctttcag 5040

gaaagagtat aagggttacg tccaatacac agccagagga caactctttc tgaggaacct 5100

accaattcta gcgacgaaga tgaagctatt aatggtggga aacctcggcg cagaaattgg 5160

cgacctggaa catctaggat gggtactgag agggccagcc gtgtgcaaaa aaattaccaa 5220

ccatgagaag tgccacgtaa acatcatgga taagctaact gcattttttg gaatcatgcc 5280

tagaggcacg acccctaggg cacctgtgag gttccccaca gcactactaa aagtgagaag 5340

ggggctagag acgggatggg cttacacgca ccaaggaggg atcagctcgg tagaccatgt 5400

cacagccgga aaggatttac tagtgtgtga cagtatgggc aggaccaggg ttgtctgtca 5460

tagtaacaat aagatgactg atgagactga gtatggcatc aagaccgact cagggtgtcc 5520

cgaaggtgcg aggtgttacg tgctaaaccc agaagctgtt aacatttctg gcacaaaagg 5580

agctatggta cacctccaga aaacgggggg ggagttcaca tgtgtcactg cctcagggac 5640

cccggctttc ttcgatctga aaaatctaaa aggctggtcc gggctaccaa tttttgaagc 5700

atccagtggc agggtggttg gtagggtgaa agtcggcaag aatgaggatt ccaagcccac 5760

caaactaatg agcggaatcc agacagtgtc taagaaccag acagacctag cggacatcgt 5820

aaaaaaattg actagtatga acagaggaga gttcaaacag ataacattag ccactggggc 5880

aggaaaaact acggaactgc caaggtccgt catagaggag atagggaggc acaaaagggt 5940

cttagtcctg ataccattga gagcagcagc agagtcagtg tatcagtata tgagagtgaa 6000

gtacccaagt atatctttca atttgagaat aggagatatg aaggaaggtg acatggccac 6060

tggtatcacc tacgcctcat atgggtactt ttgtcagctt cctcagccca aactgagagc 6120

tgccatggta gagtactcat atatattctt agatgagtac cactgtgcta cacccgagca 6180

attagcaata attggaaaga tacacaggtt tgctgaaaat cttagagtgg tagcaatgac 6240

agcaacccca gctggaacgg tcacaacgac tggtcagaaa caccctatag aggagttcat 6300

agccccagag gtgatgaaag gtgaagatct aggtagtgaa tacttggata ttgcagggtt 6360

gaagataccg actgaagaga tgaaaggcaa catgctcgtg ttcgcgccaa ctaggaacat 6420

ggcagtagaa acagctaaga aattgaaggc taagggatac aactctggat actattacag 6480

tggggaaaac ccagagaact tgagggtggt aacctcgcaa tccccgtatg tggtagtagc 6540

caccaatgcc atagagtcag gtgtgacatt accagactta gacacagttg tagacactgg 6600

actaaagtgt gagaagaggg tgaggatttc ttcaaaaatg cccttcattg taacaggact 6660

taagagaatg gcagtcacaa tcggagagca agcccagcgc aggggtagag taggaagagt 6720

caagccaggt aggtactata ggagtcaaga aacagcttca gggtcaaaag attaccatta 6780

cgacctactg caagcccaga ggtacggaat agaagatgga attaatgtaa caaagtcatt 6840

cagggagatg aactatgatt ggagccttta cgaagaggac agcttgatga taactcaact 6900

cgaggtcctt aacaacctcc ttatatcaga agacctgcct gccgcagtga agaacatcat 6960

ggcccggacc gatcacccag aacccataca actggcctat aacagttatg aaaaccaaat 7020

tccagtgctg ttcccaaaga tcaaaaatgg tgaggtgaca gacagttatg agaattacac 7080

atatctcaat gcaagaaaat taggagagga cgtgccggca tatgtgtacg ccacagagga 7140

tgaggatcta gcagtggatc ttctgggtat ggattggccg gacccaggca accaacaggt 7200

ggtagagaca gggagggcat taaaacaagt aactggctta tccacagcag aaaacgccct 7260

cttgatagcc ctattcggct acgtcgggta ccagacactt tcaaaaaggc acatacccat 7320

gattactgac atctatacac ttgaagacca caggcttgag gacacaaccc acctccagtt 7380

tgccccaaac gctataagga ccgacggcaa ggactcagag ttgaaggaat tagctgtggg 7440

agaccttgat aaatatgtgg acgcactggt agactactcc aaacaaggga tgaaattcat 7500

caaagtccaa gctgaaaagg tcagagactc ccagtctacg aaggaaggct tgcaaaccat 7560

taaggagtat gtggataagt ttatacaatc actaacagag aataaggagg agatcatcag 7620

gtatggacta tggggagttc acacggcact ctacaaaagc ttggcagcga gactggggca 7680

tgaaacagct tttgcaactt tagtggtaaa atggttggct tttgggggcg aaacggtatc 7740

tgctcacatc aagcaagtag cagttgatct agtagtatat tatatcatca acaaaccatc 7800

ttttcctgga gatacagaga cccaacaaga ggggaggaag tttgtggcta gtctttttat 7860

atctgcacta gcaacataca catataaaac ctggaattac aacaatctgc aacgggttgt 7920

cgaacctgcc ttagcttacc tcccatatgc tacaagtgcc ttgaagttgt tcacacccac 7980

aagattagag agtgtggtca tactcagttc tacaatttac aagacatacc tctctataag 8040

gaagggtaag agtgacggct tgttaggtac aggcataagt gcagccatgg agatcttaaa 8100

ccaaaaccca atctcagtag gtatatctgt gatgctgggg gtaggtgcca tcgccgccca 8160

taatgcaata gaatctagtg aacagaaaag aactttgctg atgaaggtct ttgtaaaaaa 8220

cttcttagac caagcagcaa cagatgagct agtcaaagag aaccctgaaa aaataatcat 8280

ggctctattt gaagcagtcc agaccatagg aaacccccta agactcatct accatctgta 8340

cggggtgtac tataaggggt gggaagcaaa agaactcgca gagaaaactg ctggccgcaa 8400

cttattcaca ttgatcatgt ttgaggcctt tgagctttta ggtatggact cagaaggaaa 8460

gataagaaac ttgtcaggca actacatact ggacttaatc ttcaacttgc ataataaatt 8520

aaacaagggg ctcaaaaaac tagtccttgg gtgggctcct gcacctttga gctgtgattg 8580

gacaccaagt gatgagagaa taagcctacc tcataacaac tacttaaggg tagaaaccag 8640

gtgtccttgt ggctatgaga tgaaggcaat aaaaaatgtt gctggtaaat tgacaaaagt 8700

tgaagaaaag gggtccttcc tatgcaggaa tagattaggg agaggacctc caaacttcaa 8760

agtaacaaag ttctatgatg ataacttgat agaagtcaag ccagtagcta ggctagaagg 8820

ccaggtggac ctctattaca agggagtaac agctaagtta gactacaaca atgggaaagt 8880

actgttagct accaacaagt gggaggtgga ccacgctttc ctgaccagac tagtaaagaa 8940

gcacacaggg ataggtttta aaggtgcata tttgggtgac cgaccagacc atcaagatct 9000

tgtcgataga gattgtgcaa ctataacgaa gaactcagta cagttcctaa aaatgaagaa 9060

gggttgcgct ttcacatatg acctaacaat ctctaacctt gtcaggctta ttgaactagt 9120

ccataagaat aatttacaag aaagagagat ccctaccgtg acagtaacta cttggcttgc 9180

atattctttt gtcaatgaag acctggggac tatcaagcct gtattggggg agaaagtcat 9240

cccagaaccc cccgaggagt tgagtctcca acccaccgtg agactagtca ccactgaaac 9300

agcaataacc ataacagggg aggctgaagt gatgacgaca gggatcacac cagtggtaga 9360

gatgaaagaa gaacctcagc tggaccacca gtcaactacc ctaaaggtag ggttgaagga 9420

aggggaatat ccagggccag gagttaaccc taaccattta gcagaggtga tagatgagaa 9480

agatgacagg ccttttgtcc taatcatcgg taacaaaggt tctacctcga acagagcaag 9540

aacggccaag aatatacggc tgtacaaagg aaacaaccca agagagatca gggatctgat 9600

gagccaagga agaatattga cggttgctct aaaagagttg gacccggaat taaaagaatt 9660

agtagattac aaggggacct ttctcaatag ggaagcttta gaagccctaa gcttaggtaa 9720

gccaatcaag aggaaaacca caacagcaat gatcaggagg ttaatagagc cagaggttga 9780

ggaggaacta ccagattggt tccaagcgga agaaccccta tttttggaag caaaaataca 9840

gaatgactta taccacctaa ttggcagtgt agatagtata aaaagcaaag caaaggaatt 9900

aggggccaca gataacacaa agatagtgaa ggaagttggg gctaggacct atacgatgaa 9960

attgagcagc tggagcacac aagttacaaa aaaacagatg agtctagccc ctctctttga 10020

agagctgtta ttaaagtgcc ctccatgtag taaaatttca aagggacata tggtgtcagc 10080

ataccaactg gctcaaggaa actgggaacc cctcgggtgt ggggtctata tgggaaccat 10140

accagctagg cgtctcaaga tccaccctta tgaggcttac cttaaactca aagagctggt 10200

ggaagttgaa tcttcgaggg ccactgcaaa agaatccatc ataagagaac ataacacctg 10260

gatcctgcgg aaggtgagac atgaagggaa cctaagaacc aaatcaatga tcaaccctgg 10320

gaaaatatca gatcagctat gcagagatgg acacaaaaga aacatatata ataagatcat 10380

aggctcaaca atggcctctg ctggtattag gctggagaaa ctgccagtag tccgagccca 10440

aactgacaca accagtttcc accaagccat aagagaaaaa attgataaaa cagaaaacaa 10500

gcagacccct gaattgcatg aagaactaat gaaggtcttc gactgcttaa agatcccaga 10560

gctgaaggaa tcgtatgatg aagtttcatg ggaacaatta gaagccggga taaaccgtaa 10620

gggtgcagca ggctatctag agagcaagaa cataggggaa gtcctagaca cagagaaaca 10680

catagtagag cagctgatca aggatctgag gaaggggaag aagattaggt actatgaaac 10740

agccatcccc aagaatgaga agagagacgt cagcgacgac tgggaagccg gagagttcgt 10800

tgatgaaaag aaaccaagag taatccagta cccggacgcc aaggtgagac tggccattac 10860

aaaagtgatg tacaaatggg taaagcaaaa accagtggtg atacccggct atgaaggtaa 10920

aacacctcta tttgacatat tcaacaaagt gaagaaggaa tgggattcat tccaggaccc 10980

cgtagcagtg agctttgaca ccaaagcgtg ggatacacaa gtcaccagta gagacctaat 11040

gttgataaag gatatccaga aatattattt caagagaagt atacacaaat ttttagatac 11100

aataacagaa cacatggtgg aggtacctgt cattacagca gacggtgaag tttacataag 11160

gaatggtcag aggggtagtg gccaacccga cacaagtgct ggtaatagta tgttgaatgt 11220

cctaaccatg atatatgctt tctgtaaaag tacaggcata ccttacaggg gattcagcag 11280

agtggcaaga atccatgtgt gtggtgatga tggctttttg ataacagaga gaggactggg 11340

actgaaattc tctgagaagg gtatgcagat attacatgag gccgggaagc cccagaaaat 11400

aactgaaggg gacaaaatga aagtggcata cagattcgag gacatagagt tttgttccca 11460

tactcccgtg ccagtcagat gggcagataa caccagtagt tacatggcag ggaggagcac 11520

agccactata ctagctaaga tggcaaccag gctggattcc agcggagaga ggggtagcac 11580

agcttatgag aaggccgtag ccttcagctt ccttttgatg tactcatgga atcccgtagt 11640

tagaaggatc tgcttactgg tgttgtcaca gtttccagaa atatccccat ccaaaaacac 11700

aatatactac taccaagggg atcccatagc tgcgtacaga gaagtgatag ggaaacagct 11760

gtgtgaactg aaaagaacag gatttgagaa gctggctggt ctgaatttga gtatgaccac 11820

tctaggcatc tggacaaaac atactagtaa aagactaatc caagcctgtg tagaaatagg 11880

taagagagaa ggtacctggt tagttaatgc tgacagactg attgcaggaa agactgggaa 11940

gttttacatc ccaagcactg gtgtcactct gttgggaaaa cactatgagg aaattaactt 12000

aaagcaaaag gcggcacaac cgccgataga gggggttgac agatataagt tgggccccat 12060

agttaatgtt atcttgagaa ggctgagggt gatgctgatg acagttgcca gcggaagctg 12120

gtgaatccgt ccggagcgtc gtgccctcac tcaaggtttt taattgtaaa tattgtaaat 12180

agacagctaa gatatttatt gtagttggat agtaatgcag tgatagtaaa taccccaatt 12240

taacactacc tccaatgcac taagcacttt agctgtgtga ggttaactcg acgtccacgg 12300

ttggactagg gaagacctct aacagcccc 12329

<210>4

<211>11837

<212>DNA

<213>人工序列：突变的BVDV：XIKE-B-NdN

<400>4

gtatacgaga ttagctaaag aactcgtata tggattggac gtcaacaaat ttttaattgg 60

caacgtaggg aaccttcccc tcagcgaagg ccgaaaagag gctagccatg cccttagtag 120

gactagcaaa agtaggggac tagcggtagc agtgagttcg ttggatggcc gaacccctga 180

gtacagggga gtcgtcaatg gttcgacact ccattagtcg aggagtctcg agatgccatg 240

tggacgaggg catgcccacg gcacatctta acccatgcgg gggttgcatg ggtgaaagcg 300

ctattcgtgg cgttatggac acagcctgat agggtgtagc agagacctgc tattccgcta 360

gtaaaaactc tgctgtacat ggcacatgga gttgttttcc gatgaaggga gcaagggtgc 420

tacaagtaag aagcagccta agccagatag gatagaaaaa ggtaagatga aaatagcccc 480

aaaagagaca gaaaaagatt gcaaaaccag accccccgac gcgactatag tagtagaagg 540

ggttaagtac caggtgaaga aaaaaggaaa ggtaagggga aaaaatactc aagatgggtt 600

atatcacaac aagaataagc cccctgaatc aagaaaaaaa ttggaaaagg cactgctggc 660

ttgggccatc ttagcagcgg tcctgcttca gctggtaaca ggagagaata tcacccagtg 720

gaacttgatg gacaacggca ccgagggaat acagcaagcg atgttcctaa gaggggtgaa 780

caggagtcta catggaattt ggccagagaa aatttgcacc ggagtaccaa ctcacttagc 840

aacagactat gagcttaaag agatagtggg gatgatggac gcgagtgaga agaccaacta 900

cacgtgttgc aggttgcaaa gacatgagtg gaataaaggt tggtgtaact ggtttcatat 960

agaaccgtgg atatggttga tgaacaaaac ccaaaacaac ctgacagaag ggcaaccgct 1020

tagggagtgt gctgtgactt gtaggtatga caaggaaaca gaattgaaca tcgtgacaca 1080

ggctagggac agacctacaa ctctgacagg ttgcaagaaa ggcaagaatt tctctttcgc 1140

aggtgttata ctggatgggc cctgtaactt taaagtatcg gttgaagatg tgctgttcaa 1200

ggagcacgat tgcggcaaca tgctgcaaga gaccgcgata cagctactcg atggggcaac 1260

caacaccatt gagggagcaa gggtagggac ggccaagttg acaacctggt tagggaagca 1320

attagggatc cttggtaaga agttggagaa caaaagcaaa gcatggtttg gtgcacatgc 1380

agcaagtcca tactgcggag tggagaggaa gatcggttac gtatggtata caaaaaactg 1440

cactccagct tgccttccaa gaaacactag aataataggc cccgggaaat ttgataccaa 1500

cgccgaagat ggaaaaatac tccatgagat gggggggcac ctctcagaat ttgtcctatt 1560

gtccttggtg gttctgtctg actttgcccc ggaaaccgcg agcgtcatct acttggttct 1620

acattttgcg atcccgcaaa gccacgttga tgtagacaca tgcgacaaga accagctgaa 1680

tttaacggta gcaaccacag tagcagaggt cataccaggg acagtgtgga acctagggaa 1740

gtatgtctgc ataagaccag actggtggcc atatgagacg acgacagtct tcgtcataga 1800

ggaagcaggg caagtaatca aattgatgct aagggccatc agagacttaa ctaggatatg 1860

gaatgctgcc actaccacag ctttcttaat ctttttagta aaagcactga ggggacaact 1920

aatccaaggg ctattgtggc tgatgctaat aacaggagca cagggcttcc ctgaatgcaa 1980

agagggcttc caatatgcca tatctaaaga caggaaaatg gggttattgg ggccagagag 2040

cttaactaca acatggcacc tccccaccaa aaaaatagtg gattccatgg tgcatgtatg 2100

gtgtgaagga aaagacttga aaatattaaa aatgtgcaca aaggaagaga ggtatctagt 2160

ggctgtgcac gagagagcct tatcaaccag tgccgagttt atgcagatca gtgatgggac 2220

aataggccca gacgtgatag atatgcctga tgactttgag tttggactct gcccttgtga 2280

ctcaaaacca gtgataaagg gcaaatttaa tgccagctta ctgaatggac cagctttcca 2340

gatggtatgc ccacaggggt ggactggtac aatagaatgc accctagcga accaagacac 2400

cttggacaca actgtcatta ggacatatag aagaactacc ccatttcagc ggagaaaatg 2460

gtgtacctat gaaaaaataa taggggaaga tatctatgaa tgcattctag gtggaaactg 2520

gacatgcata accggtgacc atagcaggtt gaaagacgga cctatcaaga agtgtaagtg 2580

gtgtggccat gacttcgtca actcagaggg gctaccacac tacccaatag gcaagtgcat 2640

gctcatcaac gagagtgggt acaggtatgt agatgacacc tcttgcgata ggggtggtgt 2700

agccatagtt ccatctggca ccgtaaagtg tagaataggt aacgtcacgg tgcaagttat 2760

cgctactaac aatgatctgg gacccatgcc ttgcagccca gctgaagtga tagcaagtga 2820

aggaccagtg gaaaagactg catgcacatt caactattca aggactctac ctaataagta 2880

ttatgagcca agggaccggt acttccaaca atacatgtta aaaggggagt ggcaatattg 2940

gttcgacctg gattctgtag accaccacaa agactacttc tcagagttca taatcatagc 3000

agtggtcgcc ttgttgggtg gtaagtacgt actgtggctc ttgataacat acacaatact 3060

gtctgagcag atggctatgg gtgctggagt gaatactgaa gagatagtca tgataggcaa 3120

tttgctgaca gacagtgata ttgaggttgt ggtttatttc cttcttctgt acttaatagt 3180

taaagaggaa ctggcgagga aatggattat actggtatac cacatccttg tagccaaccc 3240

tatgaaaaca attggggtcg tcttactaat gctaggggga gtggtgaagg ccagcagaat 3300

caatgctgat gaccaaagtg ctatggaccc atgctttctt ctcgtgacag gcgtagtggc 3360

tgttttgatg atcgctagaa gagaacctgc cacattacca ctgattgtag cattgctagc 3420

aataagaaca tcaggattcc tactgcccgc tagcattgat gtaactgtag cagtagtatt 3480

aattgtactt ttgttggcta gctacataac agactacttt agatataaaa agtggcttca 3540

actcttattt agtctgatag ctggtatctt tattataagg agcttaaaac atatcaacca 3600

gatggaggta ccagaaatat ctatgccaag ttggagacct ctagctctgg tccttttcta 3660

tataacatct acagcaataa ccactaattg ggacattgac ttagcaggct tcctgctgca 3720

atgggcgcca gcagtgatca tgatggctac catgtgggca gactttttga ctctgatcat 3780

agtcctgccc agttacgagt tatctaagct ttacttccta aagaacgtca ggacagacgt 3840

ggaaaagaac tggctcggca aagtgaaata cagacagatc agttcagttt atgacatctg 3900

tgacagtgag gaagcagtgt acctatttcc atcaaggcat aagagtggaa gcaggccaga 3960

tttcatatta ccttttttga aagccgtgtt aataagctgc atcagcagcc aatggcaagt 4020

ggtttacatt tcttacctaa tactggaaat tacatactat atgcacagga aaatcataga 4080

tgaggtgtca ggaggagcaa attttctatc aagactcata gcagccatca tagaattaaa 4140

ttgggccata gatgatgagg aatgtaaagg actgaagaaa ctgtatctct tgtcagggag 4200

agcgaagaat ttgatagtta aacataaggt aagaaatgaa gccgtccaca gatggtttgg 4260

tgaggaggaa atatacgggg cacccaaggt gatcactatc ataaaagcta gtaccctaag 4320

taaaaacagg cactgcataa tctgcacgat ctgtgaaggg aaagaatgga atggagccaa 4380

ctgcccaaag tgtggaagac aaggaaagcc cataacatgt ggaatgacac tcgcagactt 4440

tgaggagaaa cattacaaaa agatatttat aagagaagaa tcttcttgtc ctgtgccttt 4500

tgatccttct tgccattgta attattttcg ccacgatggg cctttcagga aagagtataa 4560

gggttacgtc caatacacag ccagaggaca actctttctg aggaacctac caattctagc 4620

gacgaagatg aagctattaa tggtgggaaa cctcggcgca gaaattggcg acctggaaca 4680

tctaggatgg gtactgagag ggccagccgt gtgcaaaaaa attaccaacc atgagaagtg 4740

ccacgtaaac atcatggata agctaactgc attttttgga atcatgccta gaggcacgac 4800

ccctagggca cctgtgaggt tccccacagc actactaaaa gtgagaaggg ggctagagac 4860

gggatgggct tacacgcacc aaggagggat cagctcggta gaccatgtca cagccggaaa 4920

ggatttacta gtgtgtgaca gtatgggcag gaccagggtt gtctgtcata gtaacaataa 4980

gatgactgat gagactgagt atggcatcaa gaccgactca gggtgtcccg aaggtgcgag 5040

gtgttacgtg ctaaacccag aagctgttaa catttctggc acaaaaggag ctatggtaca 5100

cctccagaaa acgggggggg agttcacatg tgtcactgcc tcagggaccc cggctttctt 5160

cgatctgaaa aatctaaaag gctggtccgg gctaccaatt tttgaagcat ccagtggcag 5220

ggtggttggt agggtgaaag tcggcaagaa tgaggattcc aagcccacca aactaatgag 5280

cggaatccag acagtgtcta agaaccagac agacctagcg gacatcgtaa aaaaattgac 5340

tagtatgaac agaggagagt tcaaacagat aacattagcc actggggcag gaaaaactac 5400

ggaactgcca aggtccgtca tagaggagat agggaggcac aaaagggtct tagtcctgat 5460

accattgaga gcagcagcag agtcagtgta tcagtatatg agagtgaagt acccaagtat 5520

atctttcaat ttgagaatag gagatatgaa ggaaggtgac atggccactg gtatcaccta 5580

cgcctcatat gggtactttt gtcagcttcc tcagcccaaa ctgagagctg ccatggtaga 5640

gtactcatat atattcttag atgagtacca ctgtgctaca cccgagcaat tagcaataat 5700

tggaaagata cacaggtttg ctgaaaatct tagagtggta gcaatgacag caaccccagc 5760

tggaacggtc acaacgactg gtcagaaaca ccctatagag gagttcatag ccccagaggt 5820

gatgaaaggt gaagatctag gtagtgaata cttggatatt gcagggttga agataccgac 5880

tgaagagatg aaaggcaaca tgctcgtgtt cgcgccaact aggaacatgg cagtagaaac 5940

agctaagaaa ttgaaggcta agggatacaa ctctggatac tattacagtg gggaaaaccc 6000

agagaacttg agggtggtaa cctcgcaatc cccgtatgtg gtagtagcca ccaatgccat 6060

agagtcaggt gtgacattac cagacttaga cacagttgta gacactggac taaagtgtga 6120

gaagagggtg aggatttctt caaaaatgcc cttcattgta acaggactta agagaatggc 6180

agtcacaatc ggagagcaag cccagcgcag gggtagagta ggaagagtca agccaggtag 6240

gtactatagg agtcaagaaa cagcttcagg gtcaaaagat taccattacg acctactgca 6300

agcccagagg tacggaatag aagatggaat taatgtaaca aagtcattca gggagatgaa 6360

ctatgattgg agcctttacg aagaggacag cttgatgata actcaactcg aggtccttaa 6420

caacctcctt atatcagaag acctgcctgc cgcagtgaag aacatcatgg cccggaccga 6480

tcacccagaa cccatacaac tggcctataa cagttatgaa aaccaaattc cagtgctgtt 6540

cccaaagatc aaaaatggtg aggtgacaga cagttatgag aattacacat atctcaatgc 6600

aagaaaatta ggagaggacg tgccggcata tgtgtacgcc acagaggatg aggatctagc 6660

agtggatctt ctgggtatgg attggccgga cccaggcaac caacaggtgg tagagacagg 6720

gagggcatta aaacaagtaa ctggcttatc cacagcagaa aacgccctct tgatagccct 6780

attcggctac gtcgggtacc agacactttc aaaaaggcac atacccatga ttactgacat 6840

ctatacactt gaagaccaca ggcttgagga cacaacccac ctccagtttg ccccaaacgc 6900

tataaggacc gacggcaagg actcagagtt gaaggaatta gctgtgggag accttgataa 6960

atatgtggac gcactggtag actactccaa acaagggatg aaattcatca aagtccaagc 7020

tgaaaaggtc agagactccc agtctacgaa ggaaggcttg caaaccatta aggagtatgt 7080

ggataagttt atacaatcac taacagagaa taaggaggag atcatcaggt atggactatg 7140

gggagttcac acggcactct acaaaagctt ggcagcgaga ctggggcatg aaacagcttt 7200

tgcaacttta gtggtaaaat ggttggcttt tgggggcgaa acggtatctg ctcacatcaa 7260

gcaagtagca gttgatctag tagtatatta tatcatcaac aaaccatctt ttcctggaga 7320

tacagagacc caacaagagg ggaggaagtt tgtggctagt ctttttatat ctgcactagc 7380

aacatacaca tataaaacct ggaattacaa caatctgcaa cgggttgtcg aacctgcctt 7440

agcttacctc ccatatgcta caagtgcctt gaagttgttc acacccacaa gattagagag 7500

tgtggtcata ctcagttcta caatttacaa gacatacctc tctataagga agggtaagag 7560

tgacggcttg ttaggtacag gcataagtgc agccatggag atcttaaacc aaaacccaat 7620

ctcagtaggt atatctgtga tgctgggggt aggtgccatc gccgcccata atgcaataga 7680

atctagtgaa cagaaaagaa ctttgctgat gaaggtcttt gtaaaaaact tcttagacca 7740

agcagcaaca gatgagctag tcaaagagaa ccctgaaaaa ataatcatgg ctctatttga 7800

agcagtccag accataggaa accccctaag actcatctac catctgtacg gggtgtacta 7860

taaggggtgg gaagcaaaag aactcgcaga gaaaactgct ggccgcaact tattcacatt 7920

gatcatgttt gaggcctttg agcttttagg tatggactca gaaggaaaga taagaaactt 7980

gtcaggcaac tacatactgg acttaatctt caacttgcat aataaattaa acaaggggct 8040

caaaaaacta gtccttgggt gggctcctgc acctttgagc tgtgattgga caccaagtga 8100

tgagagaata agcctacctc ataacaacta cttaagggta gaaaccaggt gtccttgtgg 8160

ctatgagatg aaggcaataa aaaatgttgc tggtaaattg acaaaagttg aagaaaaggg 8220

gtccttccta tgcaggaata gattagggag aggacctcca aacttcaaag taacaaagtt 8280

ctatgatgat aacttgatag aagtcaagcc agtagctagg ctagaaggcc aggtggacct 8340

ctattacaag ggagtaacag ctaagttaga ctacaacaat gggaaagtac tgttagctac 8400

caacaagtgg gaggtggacc acgctttcct gaccagacta gtaaagaagc acacagggat 8460

aggttttaaa ggtgcatatt tgggtgaccg accagaccat caagatcttg tcgatagaga 8520

ttgtgcaact ataacgaaga actcagtaca gttcctaaaa atgaagaagg gttgcgcttt 8580

cacatatgac ctaacaatct ctaaccttgt caggcttatt gaactagtcc ataagaataa 8640

tttacaagaa agagagatcc ctaccgtgac agtaactact tggcttgcat attcttttgt 8700

caatgaagac ctggggacta tcaagcctgt attgggggag aaagtcatcc cagaaccccc 8760

cgaggagttg agtctccaac ccaccgtgag actagtcacc actgaaacag caataaccat 8820

aacaggggag gctgaagtga tgacgacagg gatcacacca gtggtagaga tgaaagaaga 8880

acctcagctg gaccaccagt caactaccct aaaggtaggg ttgaaggaag gggaatatcc 8940

agggccagga gttaacccta accatttagc agaggtgata gatgagaaag atgacaggcc 9000

ttttgtccta atcatcggta acaaaggttc tacctcgaac agagcaagaa cggccaagaa 9060

tatacggctg tacaaaggaa acaacccaag agagatcagg gatctgatga gccaaggaag 9120

aatattgacg gttgctctaa aagagttgga cccggaatta aaagaattag tagattacaa 9180

ggggaccttt ctcaataggg aagctttaga agccctaagc ttaggtaagc caatcaagag 9240

gaaaaccaca acagcaatga tcaggaggtt aatagagcca gaggttgagg aggaactacc 9300

agattggttc caagcggaag aacccctatt tttggaagca aaaatacaga atgacttata 9360

ccacctaatt ggcagtgtag atagtataaa aagcaaagca aaggaattag gggccacaga 9420

taacacaaag atagtgaagg aagttggggc taggacctat acgatgaaat tgagcagctg 9480

gagcacacaa gttacaaaaa aacagatgag tctagcccct ctctttgaag agctgttatt 9540

aaagtgccct ccatgtagta aaatttcaaa gggacatatg gtgtcagcat accaactggc 9600

tcaaggaaac tgggaacccc tcgggtgtgg ggtctatatg ggaaccatac cagctaggcg 9660

tctcaagatc cacccttatg aggcttacct taaactcaaa gagctggtgg aagttgaatc 9720

ttcgagggcc actgcaaaag aatccatcat aagagaacat aacacctgga tcctgcggaa 9780

ggtgagacat gaagggaacc taagaaccaa atcaatgatc aaccctggga aaatatcaga 9840

tcagctatgc agagatggac acaaaagaaa catatataat aagatcatag gctcaacaat 9900

ggcctctgct ggtattaggc tggagaaact gccagtagtc cgagcccaaa ctgacacaac 9960

cagtttccac caagccataa gagaaaaaat tgataaaaca gaaaacaagc agacccctga 10020

attgcatgaa gaactaatga aggtcttcga ctgcttaaag atcccagagc tgaaggaatc 10080

gtatgatgaa gtttcatggg aacaattaga agccgggata aaccgtaagg gtgcagcagg 10140

ctatctagag agcaagaaca taggggaagt cctagacaca gagaaacaca tagtagagca 10200

gctgatcaag gatctgagga aggggaagaa gattaggtac tatgaaacag ccatccccaa 10260

gaatgagaag agagacgtca gcgacgactg ggaagccgga gagttcgttg atgaaaagaa 10320

accaagagta atccagtacc cggacgccaa ggtgagactg gccattacaa aagtgatgta 10380

caaatgggta aagcaaaaac cagtggtgat acccggctat gaaggtaaaa cacctctatt 10440

tgacatattc aacaaagtga agaaggaatg ggattcattc caggaccccg tagcagtgag 10500

ctttgacacc aaagcgtggg atacacaagt caccagtaga gacctaatgt tgataaagga 10560

tatccagaaa tattatttca agagaagtat acacaaattt ttagatacaa taacagaaca 10620

catggtggag gtacctgtca ttacagcaga cggtgaagtt tacataagga atggtcagag 10680

gggtagtggc caacccgaca caagtgctgg taatagtatg ttgaatgtcc taaccatgat 10740

atatgctttc tgtaaaagta caggcatacc ttacagggga ttcagcagag tggcaagaat 10800

ccatgtgtgt ggtgatgatg gctttttgat aacagagaga ggactgggac tgaaattctc 10860

tgagaagggt atgcagatat tacatgaggc cgggaagccc cagaaaataa ctgaagggga 10920

caaaatgaaa gtggcataca gattcgagga catagagttt tgttcccata ctcccgtgcc 10980

agtcagatgg gcagataaca ccagtagtta catggcaggg aggagcacag ccactatact 11040

agctaagatg gcaaccaggc tggattccag cggagagagg ggtagcacag cttatgagaa 11100

ggccgtagcc ttcagcttcc ttttgatgta ctcatggaat cccgtagtta gaaggatctg 11160

cttactggtg ttgtcacagt ttccagaaat atccccatcc aaaaacacaa tatactacta 11220

ccaaggggat cccatagctg cgtacagaga agtgataggg aaacagctgt gtgaactgaa 11280

aagaacagga tttgagaagc tggctggtct gaatttgagt atgaccactc taggcatctg 11340

gacaaaacat actagtaaaa gactaatcca agcctgtgta gaaataggta agagagaagg 11400

tacctggtta gttaatgctg acagactgat tgcaggaaag actgggaagt tttacatccc 11460

aagcactggt gtcactctgt tgggaaaaca ctatgaggaa attaacttaa agcaaaaggc 11520

ggcacaaccg ccgatagagg gggttgacag atataagttg ggccccatag ttaatgttat 11580

cttgagaagg ctgagggtga tgctgatgac agttgccagc ggaagctggt gaatccgtcc 11640

ggagcgtcgt gccctcactc aaggttttta attgtaaata ttgtaaatag acagctaaga 11700

tatttattgt agttggatag taatgcagtg atagtaaata ccccaattta acactacctc 11760

caatgcacta agcactttag ctgtgtgagg ttaactcgac gtccacggtt ggactaggga 11820

agacctctaa cagcccc 11837

<210>5

<211>3913

<212>PRT

<213>野生型BVDV：XIKE-A

<400>5

Met Glu Leu Phe Ser Asn Glu Leu Leu Tyr Lys Thr Tyr Lys Gln Lys

1 5 10 15

Pro Ala Gly Val Val Glu Pro Val Tyr Asp Val Asn Gly Arg Pro Leu

20 25 30

Phe Gly Glu Ser Ser Asp Leu His Pro Gln Ser Thr Leu Lys Leu Pro

35 40 45

His Gln Arg Gly Ser Ala Asn Ile Leu Thr Asn Ala Arg Ser Leu Pro

50 55 60

Arg Lys Gly Asp Cys Arg Arg Gly Asn Val Tyr Gly Pro Val Ser Gly

65 70 75 80

Ile Tyr Ile Lys Pro Gly Pro Ile Tyr Tyr Gln Asp Tyr Val Gly Pro

85 90 95

Val Tyr His Arg Ala Pro Leu Glu Leu Cys Arg Glu Ala Ser Met Cys

100 105 110

Glu Thr Thr Arg Arg Val Gly Arg Val Thr Gly Ser Asp Gly Lys Leu

115 120 125

Tyr His Ile Tyr Ile Cys Ile Asp Gly Cys Ile Leu Leu Lys Arg Ala

130 135 140

Thr Arg Asn Gln Pro Glu Val Leu Lys Trp Val Tyr Asn Arg Leu Asn

145 150 155 160

Cys Pro Leu Trp Val Thr Ser Cys Ser Asp Glu Gly Ser Lys Gly Ala

165 170 175

Thr Ser Lys Lys Gln Pro Lys Pro Asp Arg Ile Glu Lys Gly Lys Met

180 185 190

Lys Ile Ala Pro Lys Glu Thr Glu Lys Asp Cys Lys Thr Arg Pro Pro

195 200 205

Asp Ala Thr Ile Val Val Glu Gly Val Lys Tyr Gln Val Lys Lys Lys

210 215 220

Gly Lys Val Arg Gly Lys Asn Thr Gln Asp Gly Leu Tyr His Asn Lys

225 230 235 240

Asn Lys Pro Pro Glu Ser Arg Lys Lys Leu Glu Lys Ala Leu Leu Ala

245 250 255

Trp Ala Ile Leu Ala Ala Val Leu Leu Gln Leu Val Thr Gly Glu Asn

260 265 270

Ile Thr Gln Trp Asn Leu Met Asp Asn Gly Thr Glu Gly Ile Gln Gln

275 280 285

Ala Met Phe Leu Arg Gly Val Asn Arg Ser Leu His Gly Ile Trp Pro

290 295 300

Glu Lys Ile Cys Thr Gly Val Pro Thr His Leu Ala Thr Asp Tyr Glu

305 310 315 320

Leu Lys Glu Ile Val Gly Met Met Asp Ala Ser Glu Lys Thr Asn Tyr

325 330 335

Thr Cys Cys Arg Leu Gln Arg His Glu Trp Asn Lys His Gly Trp Cys

340 345 350

Asn Trp Phe His Ile Glu Pro Trp Ile Trp Leu Met Asn Lys Thr Gln

355 360 365

Asn Asn Leu Thr Glu Gly Gln Pro Leu Arg Glu Cys Ala Val Thr Cys

370 375 380

Arg Tyr Asp Lys Glu Thr Glu Leu Asn Ile Val Thr Gln Ala Arg Asp

385 390 395 400

Arg Pro Thr Thr Leu Thr Gly Cys Lys Lys Gly Lys Asn Phe Ser Phe

405 410 415

Ala Gly Val Ile Leu Asp Gly Pro Cys Asn Phe Lys Val Ser Val Glu

420 425 430

Asp Val Leu Phe Lys Glu His Asp Cys Gly Asn Met Leu Gln Glu Thr

435 440 445

Ala Ile Gln Leu Leu Asp Gly Ala Thr Asn Thr Ile Glu Gly Ala Arg

450 455 460

Val Gly Thr Ala Lys Leu Thr Thr Trp Leu Gly Lys Gln Leu Gly Ile

465 470 475 480

Leu Gly Lys Lys Leu Glu Asn Lys Ser Lys Ala Trp Phe Gly Ala His

485 490 495

Ala Ala Ser Pro Tyr Cys Gly Val Glu Arg Lys Ile Gly Tyr Val Trp

500 505 510

Tyr Thr Lys Asn Cys Thr Pro Ala Cys Leu Pro Arg Asn Thr Arg Ile

515 520 525

Ile Gly Pro Gly Lys Phe Asp Thr Asn Ala Glu Asp Gly Lys Ile Leu

530 535 540

His Glu Met Gly Gly His Leu Ser Glu Phe Val Leu Leu Ser Leu Val

545 550 555 560

Val Leu Ser Asp Phe Ala Pro Glu Thr Ala Ser Val Ile Tyr Leu Val

565 570 575

Leu His Phe Ala Ile Pro Gln Ser His Val Asp Val Asp Thr Cys Asp

580 585 590

Lys Asn Gln Leu Asn Leu Thr Val Ala Thr Thr Val Ala Glu Val Ile

595 600 605

Pro Gly Thr Val Trp Asn Leu Gly Lys Tyr Val Cys Ile Arg Pro Asp

610 615 620

Trp Trp Pro Tyr Glu Thr Thr Thr Val Phe Val Ile Glu Glu Ala Gly

625 630 635 640

Gln Val Ile Lys Leu Met Leu Arg Ala Ile Arg Asp Leu Thr Arg Ile

645 650 655

Trp Asn Ala Ala Thr Thr Thr Ala Phe Leu Ile Phe Leu Val Lys Ala

660 665 670

Leu Arg Gly Gln Leu Ile Gln Gly Leu Leu Trp Leu Met Leu Ile Thr

675 680 685

Gly Ala Gln Gly Phe Pro Glu Cys Lys Glu Gly Phe Gln Tyr Ala Ile

690 695 700

Ser Lys Asp Arg Lys Met Gly Leu Leu Gly Pro Glu Ser Leu Thr Thr

705 710 715 720

Thr Trp His Leu Pro Thr Lys Lys Ile Val Asp Ser Met Val His Val

725 730 735

Trp Cys Glu Gly Lys Asp Leu Lys Ile Leu Lys Met Cys Thr Lys Glu

740 745 750

Glu Arg Tyr Leu Val Ala Val His Glu Arg Ala Leu Ser Thr Ser Ala

755 760 765

Glu Phe Met Gln Ile Ser Asp Gly Thr Ile Gly Pro Asp Val Ile Asp

770 775 780

Met Pro Asp Asp Phe Glu Phe Gly Leu Cys Pro Cys Asp Ser Lys Pro

785 790 795 800

Val Ile Lys Gly Lys Phe Asn Ala Ser Leu Leu Asn Gly Pro Ala Phe

805 810 815

Gln Met Val Cys Pro Gln Gly Trp Thr Gly Thr Ile Glu Cys Thr Leu

820 825 830

Ala Asn Gln Asp Thr Leu Asp Thr Thr Val Ile Arg Thr Tyr Arg Arg

835 840 845

Thr Thr Pro Phe Gln Arg Arg Lys Trp Cys Thr Tyr Glu Lys Ile Ile

850 855 860

Gly Glu Asp Ile Tyr Glu Cys Ile Leu Gly Gly Asn Trp Thr Cys Ile

865 870 875 880

Thr Gly Asp His Ser Arg Leu Lys Asp Gly Pro Ile Lys Lys Cys Lys

885 890 895

Trp Cys Gly His Asp Phe Val Asn Ser Glu Gly Leu Pro His Tyr Pro

900 905 910

Ile Gly Lys Cys Met Leu Ile Asn Glu Ser Gly Tyr Arg Tyr Val Asp

915 920 925

Asp Thr Ser Cys Asp Arg Gly Gly Val Ala Ile Val Pro Ser Gly Thr

930 935 940

Val Lys Cys Arg Ile Gly Asn Val Thr Val Gln Val Ile Ala Thr Asn

945 950 955 960

Asn Asp Leu Gly Pro Met Pro Cys Ser Pro Ala Glu Val Ile Ala Ser

965 970 975

Glu Gly Pro Val Glu Lys Thr Ala Cys Thr Phe Asn Tyr Ser Arg Thr

980 985 990

Leu Pro Asn Lys Tyr Tyr Glu Pro Arg Asp Arg Tyr Phe Gln Gln Tyr

995 1000 1005

Met Leu Lys Gly Glu Trp Gln Tyr Trp Phe Asp Leu Asp Ser Val

1010 1015 1020

Asp His His Lys Asp Tyr Phe Ser Glu Phe Ile Ile Ile Ala Val

1025 1030 1035

Val Ala Leu Leu Gly Gly Lys Tyr Val Leu Trp Leu Leu Ile Thr

1040 1045 1050

Tyr Thr Ile Leu Ser Glu Gln Met Ala Met Gly Ala Gly Val Asn

1055 1060 1065

Thr Glu Glu Ile Val Met Ile Gly Asn Leu Leu Thr Asp Ser Asp

1070 1075 1080

Ile Glu Val Val Val Tyr Phe Leu Leu Leu Tyr Leu Ile Val Lys

1085 1090 1095

Glu Glu Leu Ala Arg Lys Trp Ile Ile Leu Val Tyr His Ile Leu

1100 1105 1110

Val Ala Asn Pro Met Lys Thr Ile Gly Val Val Leu Leu Met Leu

1115 1120 1125

Gly Gly Val Val Lys Ala Ser Arg Ile Asn Ala Asp Asp Gln Ser

1130 1135 1140

Ala Met Asp Pro Cys Phe Leu Leu Val Thr Gly Val Val Ala Val

1145 1150 1155

Leu Met Ile Ala Arg Arg Glu Pro Ala Thr Leu Pro Leu Ile Val

1160 1165 1170

Ala Leu Leu Ala Ile Arg Thr Ser Gly Phe Leu Leu Pro Ala Ser

1175 1180 1185

Ile Asp Val Thr Val Ala Val Val Leu Ile Val Leu Leu Leu Ala

1190 1195 1200

Ser Tyr Ile Thr Asp Tyr Phe Arg Tyr Lys Lys Trp Leu Gln Leu

1205 1210 1215

Leu Phe Ser Leu Ile Ala Gly Ile Phe Ile Ile Arg Ser Leu Lys

1220 1225 1230

His Ile Asn Gln Met Glu Val Pro Glu Ile Ser Met Pro Ser Trp

1235 1240 1245

Arg Pro Leu Ala Leu Val Leu Phe Tyr Ile Thr Ser Thr Ala Ile

1250 1255 1260

Thr Thr Asn Trp Asp Ile Asp Leu Ala Gly Phe Leu Leu Gln Trp

1265 1270 1275

Ala Pro Ala Val Ile Met Met Ala Thr Met Trp Ala Asp Phe Leu

1280 1285 1290

Thr Leu Ile Ile Val Leu Pro Ser Tyr Glu Leu Ser Lys Leu Tyr

1295 1300 1305

Phe Leu Lys Asn Val Arg Thr Asp Val Glu Lys Asn Trp Leu Gly

1310 1315 1320

Lys Val Lys Tyr Arg Gln Ile Ser Ser Val Tyr Asp Ile Cys Asp

1325 1330 1335

Ser Glu Glu Ala Val Tyr Leu Phe Pro Ser Arg His Lys Ser Gly

1340 1345 1350

Ser Arg Pro Asp Phe Ile Leu Pro Phe Leu Lys Ala Val Leu Ile

1355 1360 1365

Ser Cys Ile Ser Ser Gln Trp Gln Val Val Tyr Ile Ser Tyr Leu

1370 1375 1380

Ile Leu Glu Ile Thr Tyr Tyr Met His Arg Lys Ile Ile Asp Glu

1385 1390 1395

Val Ser Gly Gly Ala Asn Phe Leu Ser Arg Leu Ile Ala Ala Ile

1400 1405 1410

Ile Glu Leu Asn Trp Ala Ile Asp Asp Glu Glu Cys Lys Gly Leu

1415 1420 1425

Lys Lys Leu Tyr Leu Leu Ser Gly Arg Ala Lys Asn Leu Ile Val

1430 1435 1440

Lys His Lys Val Arg Asn Glu Ala Val His Arg Trp Phe Gly Glu

1445 1450 1455

Glu Glu Ile Tyr Gly Ala Pro Lys Val Ile Thr Ile Ile Lys Ala

1460 1465 1470

Ser Thr Leu Ser Lys Asn Arg His Cys Ile Ile Cys Thr Ile Cys

1475 1480 1485

Glu Gly Lys Glu Trp Asn Gly Ala Asn Cys Pro Lys Cys Gly Arg

1490 1495 1500

Gln Gly Lys Pro Ile Thr Cys Gly Met Thr Leu Ala Asp Phe Glu

1505 1510 1515

Glu Lys His Tyr Lys Lys Ile Phe Ile Arg Glu Glu Ser Ser Cys

1520 1525 1530

Pro Val Pro Phe Asp Pro Ser Cys His Cys Asn Tyr Phe Arg His

1535 1540 1545

Asp Gly Pro Phe Arg Lys Glu Tyr Lys Gly Tyr Val Gln Tyr Thr

1550 1555 1560

Ala Arg Gly Gln Leu Phe Leu Arg Asn Leu Pro Ile Leu Ala Thr

1565 1570 1575

Lys Met Lys Leu Leu Met Val Gly Asn Leu Gly Ala Glu Ile Gly

1580 1585 1590

Asp Leu Glu His Leu Gly Trp Val Leu Arg Gly Pro Ala Val Cys

1595 1600 1605

Lys Lys Ile Thr Asn His Glu Lys Cys His Val Asn Ile Met Asp

1610 1615 1620

Lys Leu Thr Ala Phe Phe Gly Ile Met Pro Arg Gly Thr Thr Pro

1625 1630 1635

Arg Ala Pro Val Arg Phe Pro Thr Ala Leu Leu Lys Val Arg Arg

1640 1645 1650

Gly Leu Glu Thr Gly Trp Ala Tyr Thr His Gln Gly Gly Ile Ser

1655 1660 1665

Ser Val Asp His Val Thr Ala Gly Lys Asp Leu Leu Val Cys Asp

1670 1675 1680

Ser Met Gly Arg Thr Arg Val Val Cys His Ser Asn Asn Lys Met

1685 1690 1695

Thr Asp Glu Thr Glu Tyr Gly Ile Lys Thr Asp Ser Gly Cys Pro

1700 1705 1710

Glu Gly Ala Arg Cys Tyr Val Leu Asn Pro Glu Ala Val Asn Ile

1715 1720 1725

Ser Gly Thr Lys Gly Ala Met Val His Leu Gln Lys Thr Gly Gly

1730 1735 1740

Glu Phe Thr Cys Val Thr Ala Ser Gly Thr Pro Ala Phe Phe Asp

1745 1750 1755

Leu Lys Asn Leu Lys Gly Trp Ser Gly Leu Pro Ile Phe Glu Ala

1760 1765 1770

Ser Ser Gly Arg Val Val Gly Arg Val Lys Val Gly Lys Asn Glu

1775 1780 1785

Asp Ser Lys Pro Thr Lys Leu Met Ser Gly Ile Gln Thr Val Ser

1790 1795 1800

Lys Asn Gln Thr Asp Leu Ala Asp Ile Val Lys Lys Leu Thr Ser

1805 1810 1815

Met Asn Arg Gly Glu Phe Lys Gln Ile Thr Leu Ala Thr Gly Ala

1820 1825 1830

Gly Lys Thr Thr Glu Leu Pro Arg Ser Val Ile Glu Glu Ile Gly

1835 1840 1845

Arg His Lys Arg Val Leu Val Leu Ile Pro Leu Arg Ala Ala Ala

1850 1855 1860

Glu Ser Val Tyr Gln Tyr Met Arg Val Lys Tyr Pro Ser Ile Ser

1865 1870 1875

Phe Asn Leu Arg Ile Gly Asp Met Lys Glu Gly Asp Met Ala Thr

1880 1885 1890

Gly Ile Thr Tyr Ala Ser Tyr Gly Tyr Phe Cys Gln Leu Pro Gln

1895 1900 1905

Pro Lys Leu Arg Ala Ala Met Val Glu Tyr Ser Tyr Ile Phe Leu

1910 1915 1920

Asp Glu Tyr His Cys Ala Thr Pro Glu Gln Leu Ala Ile Ile Gly

1925 1930 1935

Lys Ile His Arg Phe Ala Glu Asn Leu Arg Val Val Ala Met Thr

1940 1945 1950

Ala Thr Pro Ala Gly Thr Val Thr Thr Thr Gly Gln Lys His Pro

1955 1960 1965

Ile Glu Glu Phe Ile Ala Pro Glu Val Met Lys Gly Glu Asp Leu

1970 1975 1980

Gly Ser Glu Tyr Leu Asp Ile Ala Gly Leu Lys Ile Pro Thr Glu

1985 1990 1995

Glu Met Lys Gly Asn Met Leu Val Phe Ala Pro Thr Arg Asn Met

2000 2005 2010

Ala Val Glu Thr Ala Lys Lys Leu Lys Ala Lys Gly Tyr Asn Ser

2015 2020 2025

Gly Tyr Tyr Tyr Ser Gly Glu Asn Pro Glu Asn Leu Arg Val Val

2030 2035 2040

Thr Ser Gln Ser Pro Tyr Val Val Val Ala Thr Asn Ala Ile Glu

2045 2050 2055

Ser Gly Val Thr Leu Pro Asp Leu Asp Thr Val Val Asp Thr Gly

2060 2065 2070

Leu Lys Cys Glu Lys Arg Val Arg Ile Ser Ser Lys Met Pro Phe

2075 2080 2085

Ile Val Thr Gly Leu Lys Arg Met Ala Val Thr Ile Gly Glu Gln

2090 2095 2100

Ala Gln Arg Arg Gly Arg Val Gly Arg Val Lys Pro Gly Arg Tyr

2105 2110 2115

Tyr Arg Ser Gln Glu Thr Ala Ser Gly Ser Lys Asp Tyr His Tyr

2120 2125 2130

Asp Leu Leu Gln Ala Gln Arg Tyr Gly Ile Glu Asp Gly Ile Asn

2135 2140 2145

Val Thr Lys Ser Phe Arg Glu Met Asn Tyr Asp Trp Ser Leu Tyr

2150 2155 2160

Glu Glu Asp Ser Leu Met Ile Thr Gln Leu Glu Val Leu Asn Asn

2165 2170 2175

Leu Leu Ile Ser Glu Asp Leu Pro Ala Ala Val Lys Asn Ile Met

2180 2185 2190

Ala Arg Thr Asp His Pro Glu Pro Ile Gln Leu Ala Tyr Asn Ser

2195 2200 2205

Tyr Glu Asn Gln Ile Pro Val Leu Phe Pro Lys Ile Lys Asn Gly

2210 2215 2220

Glu Val Thr Asp Ser Tyr Glu Asn Tyr Thr Tyr Leu Asn Ala Arg

2225 2230 2235

Lys Leu Gly Glu Asp Val Pro Ala Tyr Val Tyr Ala Thr Glu Asp

2240 2245 2250

Glu Asp Leu Ala Val Asp Leu Leu Gly Met Asp Trp Pro Asp Pro

2255 2260 2265

Gly Asn Gln Gln Val Val Glu Thr Gly Arg Ala Leu Lys Gln Val

2270 2275 2280

Thr Gly Leu Ser Thr Ala Glu Asn Ala Leu Leu Ile Ala Leu Phe

2285 2290 2295

Gly Tyr Val Gly Tyr Gln Thr Leu Ser Lys Arg His Ile Pro Met

2300 2305 2310

Ile Thr Asp Ile Tyr Thr Leu Glu Asp His Arg Leu Glu Asp Thr

2315 2320 2325

Thr His Leu Gln Phe Ala Pro Asn Ala Ile Arg Thr Asp Gly Lys

2330 2335 2340

Asp Ser Glu Leu Lys Glu Leu Ala Val Gly Asp Leu Asp Lys Tyr

2345 2350 2355

Val Asp Ala Leu Val Asp Tyr Ser Lys Gln Gly Met Lys Phe Ile

2360 2365 2370

Lys Val Gln Ala Glu Lys Val Arg Asp Ser Gln Ser Thr Lys Glu

2375 2380 2385

Gly Leu Gln Thr Ile Lys Glu Tyr Val Asp Lys Phe Ile Gln Ser

2390 2395 2400

Leu Thr Glu Asn Lys Glu Glu Ile Ile Arg Tyr Gly Leu Trp Gly

2405 2410 2415

Val His Thr Ala Leu Tyr Lys Ser Leu Ala Ala Arg Leu Gly His

2420 2425 2430

Glu Thr Ala Phe Ala Thr Leu Val Val Lys Trp Leu Ala Phe Gly

2435 2440 2445

Gly Glu Thr Val Ser Ala His Ile Lys Gln Val Ala Val Asp Leu

2450 2455 2460

Val Val Tyr Tyr Ile Ile Asn Lys Pro Ser Phe Pro Gly Asp Thr

2465 2470 2475

Glu Thr Gln Gln Glu Gly Arg Arg Phe Val Ala Ser Leu Phe Ile

2480 2485 2490

Ser Ala Leu Ala Thr Tyr Thr Tyr Lys Thr Trp Asn Tyr Asn Asn

2495 2500 2505

Leu Gln Arg Val Val Glu Pro Ala Leu Ala Tyr Leu Pro Tyr Ala

2510 2515 2520

Thr Ser Ala Leu Lys Leu Phe Thr Pro Thr Arg Leu Glu Ser Val

2525 2530 2535

Val Ile Leu Ser Ser Thr Ile Tyr Lys Thr Tyr Leu Ser Ile Arg

2540 2545 2550

Lys Gly Lys Ser Asp Gly Leu Leu Gly Thr Gly Ile Ser Ala Ala

2555 2560 2565

Met Glu Ile Leu Asn Gln Asn Pro Ile Ser Val Gly Ile Ser Val

2570 2575 2580

Met Leu Gly Val Gly Ala Ile Ala Ala His Asn Ala Ile Glu Ser

2585 2590 2595

Ser Glu Gln Lys Arg Thr Leu Leu Met Lys Val Phe Val Lys Asn

2600 2605 2610

Phe Leu Asp Gln Ala Ala Thr Asp Glu Leu Val Lys Glu Asn Pro

2615 2620 2625

Glu Lys Ile Ile Met Ala Leu Phe Glu Ala Val Gln Thr Ile Gly

2630 2635 2640

Asn Pro Leu Arg Leu Ile Tyr His Leu Tyr Gly Val Tyr Tyr Lys

2645 2650 2655

Gly Trp Glu Ala Lys Glu Leu Ala Glu Lys Thr Ala Gly Arg Asn

2660 2665 2670

Leu Phe Thr Leu Ile Met Phe Glu Ala Phe Glu Leu Leu Gly Met

2675 2680 2685

Asp Ser Glu Gly Lys Ile Arg Asn Leu Ser Gly Asn Tyr Ile Leu

2690 2695 2700

Asp Leu Ile Phe Asn Leu His Asn Lys Leu Asn Lys Gly Leu Lys

2705 2710 2715

Lys Leu Val Leu Gly Trp Ala Pro Ala Pro Leu Ser Cys Asp Trp

2720 2725 2730

Thr Pro Ser Asp Glu Arg Ile Ser Leu Pro His Asn Asn Tyr Leu

2735 2740 2745

Arg Val Glu Thr Arg Cys Pro Cys Gly Tyr Glu Met Lys Ala Ile

2750 2755 2760

Lys Asn Val Ala Gly Lys Leu Thr Lys Val Glu Glu Lys Gly Ser

2765 2770 2775

Phe Leu Cys Arg Asn Arg Leu Gly Arg Gly Pro Pro Asn Phe Lys

2780 2785 2790

Val Thr Lys Phe Tyr Asp Asp Asn Leu Ile Glu Val Lys Pro Val

2795 2800 2805

Ala Arg Leu Glu Gly Gln Val Asp Leu Tyr Tyr Lys Gly Val Thr

2810 2815 2820

Ala Lys Leu Asp Tyr Asn Asn Gly Lys Val Leu Leu Ala Thr Asn

2825 2830 2835

Lys Trp Glu Val Asp His Ala Phe Leu Thr Arg Leu Val Lys Lys

2840 2845 2850

His Thr Gly Ile Gly Phe Lys Gly Ala Tyr Leu Gly Asp Arg Pro

2855 2860 2865

Asp His Gln Asp Leu Val Asp Arg Asp Cys Ala Thr Ile Thr Lys

2870 2875 2880

Asn Ser Val Gln Phe Leu Lys Met Lys Lys Gly Cys Ala Phe Thr

2885 2890 2895

Tyr Asp Leu Thr Ile Ser Asn Leu Val Arg Leu Ile Glu Leu Val

2900 2905 2910

His Lys Asn Asn Leu Gln Glu Arg Glu Ile Pro Thr Val Thr Val

2915 2920 2925

Thr Thr Trp Leu Ala Tyr Ser Phe Val Asn Glu Asp Leu Gly Thr

2930 2935 2940

Ile Lys Pro Val Leu Gly Glu Lys Val Ile Pro Glu Pro Pro Glu

2945 2950 2955

Glu Leu Ser Leu Gln Pro Thr Val Arg Leu Val Thr Thr Glu Thr

2960 2965 2970

Ala Ile Thr Ile Thr Gly Glu Ala Glu Val Met Thr Thr Gly Ile

2975 2980 2985

Thr Pro Val Val Glu Met Lys Glu Glu Pro Gln Leu Asp His Gln

2990 2995 3000

Ser Thr Thr Leu Lys Val Gly Leu Lys Glu Gly Glu Tyr Pro Gly

3005 3010 3015

Pro Gly Val Asn Pro Asn His Leu Ala Glu yal Ile Asp Glu Lys

3020 3025 3030

Asp Asp Arg Pro Phe Val Leu Ile Ile Gly Asn Lys Gly Ser Thr

3035 3040 3045

Ser Asn Arg Ala Arg Thr Ala Lys Asn Ile Arg Leu Tyr Lys Gly

3050 3055 3060

Asn Asn Pro Arg Glu Ile Arg Asp Leu Met Ser Gln Gly Arg Ile

3065 3070 3075

Leu Thr Val Ala Leu Lys Glu Leu Asp Pro Glu Leu Lys Glu Leu

3080 3085 3090

Val Asp Tyr Lys Gly Thr Phe Leu Asn Arg Glu Ala Leu Glu Ala

3095 3100 3105

Leu Ser Leu Gly Lys Pro Ile Lys Arg Lys Thr Thr Thr Ala Met

3110 3115 3120

Ile Arg Arg Leu Ile Glu Pro Glu Val Glu Glu Glu Leu Pro Asp

3125 3130 3135

Trp Phe Gln Ala Glu Glu Pro Leu Phe Leu Glu Ala Lys Ile Gln

3140 3145 3150

Asn Asp Leu Tyr His Leu Ile Gly Ser Val Asp Ser Ile Lys Ser

3155 3160 3165

Lys Ala Lys Glu Leu Gly Ala Thr Asp Asn Thr Lys Ile Val Lys

3170 3175 3180

Glu Val Gly Ala Arg Thr Tyr Thr Met Lys Leu Ser Ser Trp Ser

3185 3190 3195

Thr Gln Val Thr Lys Lys Gln Met Ser Leu Ala Pro Leu Phe Glu

3200 3205 3210

Glu Leu Leu Leu Lys Cys Pro Pro Cys Ser Lys Ile Ser Lys Gly

3215 3220 3225

His Met Val Ser Ala Tyr Gln Leu Ala Gln Gly Asn Trp Glu Pro

3230 3235 3240

Leu Gly Cys Gly Val Tyr Met Gly Thr Ile Pro Ala Arg Arg Leu

3245 3250 3255

Lys Ile His Pro Tyr Glu Ala Tyr Leu Lys Leu Lys Glu Leu Val

3260 3265 3270

Glu Val Glu Ser Ser Arg Ala Thr Ala Lys Glu Ser Ile Ile Arg

3275 3280 3285

Glu His Asn Thr Trp Ile Leu Arg Lys Val Arg His Glu Gly Asn

3290 3295 3300

Leu Arg Thr Lys Ser Met Ile Asn Pro Gly Lys Ile Ser Asp Gln

3305 3310 3315

Leu Cys Arg Asp Gly His Lys Arg Asn Ile Tyr Asn Lys Ile Ile

3320 3325 3330

Gly Ser Thr Met Ala Ser Ala Gly Ile Arg Leu Glu Lys Leu Pro

3335 3340 3345

Val Val Arg Ala Gln Thr Asp Thr Thr Ser Phe His Gln Ala Ile

3350 3355 3360

Arg Glu Lys Ile Asp Lys Thr Glu Asn Lys Gln Thr Pro Glu Leu

3365 3370 3375

His Glu Glu Leu Met Lys Val Phe Asp Cys Leu Lys Ile Pro Glu

3380 3385 3390

Leu Lys Glu Ser Tyr Asp Glu Val Ser Trp Glu Gln Leu Glu Ala

3395 3400 3405

Gly Ile Asn Arg Lys Gly Ala Ala Gly Tyr Leu Glu Ser Lys Asn

3410 3415 3420

Ile Gly Glu Val Leu Asp Thr Glu Lys His Ile Val Glu Gln Leu

3425 3430 3435

Ile Lys Asp Leu Arg Lys Gly Lys Lys Ile Arg Tyr Tyr Glu Thr

3440 3445 3450

Ala Ile Pro Lys Asn Glu Lys Arg Asp Val Ser Asp Asp Trp Glu

3455 3460 3465

Ala Gly Glu Phe Val Asp Glu Lys Lys Pro Arg Val Ile Gln Tyr

3470 3475 3480

Pro Asp Ala Lys Val Arg Leu Ala Ile Thr Lys Val Met Tyr Lys

3485 3490 3495

Trp Val Lys Gln Lys Pro Val Val Ile Pro Gly Tyr Glu Gly Lys

3500 3505 3510

Thr Pro Leu Phe Asp Ile Phe Asn Lys Val Lys Lys Glu Trp Asp

3515 3520 3525

Ser Phe Gln Asp Pro Val Ala Val Ser Phe Asp Thr Lys Ala Trp

3530 3535 3540

Asp Thr Gln Val Thr Ser Arg Asp Leu Met Leu Ile Lys Asp Ile

3545 3550 3555

Gln Lys Tyr Tyr Phe Lys Arg Ser Ile His Lys Phe Leu Asp Thr

3560 3565 3570

Ile Thr Glu His Met Val Glu Val Pro Val Ile Thr Ala Asp Gly

3575 3580 3585

Glu Val Tyr Ile Arg Asn Gly Gln Arg Gly Ser Gly Gln Pro Asp

3590 3595 3600

Thr Ser Ala Gly Asn Ser Met Leu Asn Val Leu Thr Met Ile Tyr

3605 3610 3615

Ala Phe Cys Lys Ser Thr Gly Ile Pro Tyr Arg Gly Phe Ser Arg

3620 3625 3630

Val Ala Arg Ile His Val Cys Gly Asp Asp Gly Phe Leu Ile Thr

3635 3640 3645

Glu Arg Gly Leu Gly Leu Lys Phe Ser Glu Lys Gly Met Gln Ile

3650 3655 3660

Leu His Glu Ala Gly Lys Pro Gln Lys Ile Thr Glu Gly Asp Lys

3665 3670 3675

Met Lys Val Ala Tyr Arg Phe Glu Asp Ile Glu Phe Cys Ser His

3680 3685 3690

Thr Pro Val Pro Val Arg Trp Ala Asp Asn Thr Ser Ser Tyr Met

3695 3700 3705

Ala Gly Arg Ser Thr Ala Thr Ile Leu Ala Lys Met Ala Thr Arg

3710 3715 3720

Leu Asp Ser Ser Gly Glu Arg Gly Ser Thr Ala Tyr Glu Lys Ala

3725 3730 3735

Val Ala Phe Ser Phe Leu Leu Met Tyr Ser Trp Asn Pro Val Val

3740 3745 3750

Arg Arg Ile Cys Leu Leu Val Leu Ser Gln Phe Pro Glu Ile Ser

3755 3760 3765

Pro Ser Lys Asn Thr Ile Tyr Tyr Tyr Gln Gly Asp Pro Ile Ala

3770 3775 3780

Ala Tyr Arg Glu Val Ile Gly Lys Gln Leu Cys Glu Leu Lys Arg

3785 3790 3795

Thr Gly Phe Glu Lys Leu Ala Gly Leu Asn Leu Ser Met Thr Thr

3800 3805 3810

Leu Gly Ile Trp Thr Lys His Thr Ser Lys Arg Leu Ile Gln Ala

3815 3820 3825

Cys Val Glu Ile Gly Lys Arg Glu Gly Thr Trp Leu Val Asn Ala

3830 3835 3840

Asp Arg Leu Ile Ala Gly Lys Thr Gly Lys Phe Tyr Ile Pro Ser

3845 3850 3855

Thr Gly Val Thr Leu Leu Gly Lys His Tyr Glu Glu Ile Asn Leu

3860 3865 3870

Lys Gln Lys Ala Ala Gln Pro Pro Ile Glu Gly Val Asp Arg Tyr

3875 3880 3885

Lys Leu Gly Pro Ile Val Asn Val Ile Leu Arg Arg Leu Arg Val

3890 3895 3900

Met Leu Met Thr Val Ala Ser Gly Ser Trp

3905 3910

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Met Glu Leu Phe Ser Asp Glu Gly Ser Lys Gly Ala Thr Ser Lys Lys

1 5 10 15

Gln Pro Lys Pro Asp Arg Ile Glu Lys Gly Lys Met Lys Ile Ala Pro

20 25 30

Lys Glu Thr Glu Lys Asp Cys Lys Thr Arg Pro Pro Asp Ala Thr Ile

35 40 45

Val Val Glu Gly Val Lys Tyr Gln Val Lys Lys Lys Gly Lys Val Arg

50 55 60

Gly Lys Asn Thr Gln Asp Gly Leu Tyr His Asn Lys Asn Lys Pro Pro

65 70 75 80

Glu Ser Arg Lys Lys Leu Glu Lys Ala Leu Leu Ala Trp Ala Ile Leu

85 90 95

Ala Ala Val Leu Leu Gln Leu Val Thr Gly Glu Asn Ile Thr Gln Trp

100 105 110

Asn Leu Met Asp Asn Gly Thr Glu Gly Ile Gln Gln Ala Met Phe Leu

115 120 125

Arg Gly Val Asn Arg Ser Leu His Gly Ile Trp Pro Glu Lys Ile Cys

130 135 140

Thr Gly Val Pro Thr His Leu Ala Thr Asp Tyr Glu Leu Lys Glu Ile

145 150 155 160

Val Gly Met Met Asp Ala Ser Glu Lys Thr Asn Tyr Thr Cys Cys Arg

165 170 175

Leu Gln Arg His Glu Trp Asn Lys His Gly Trp Cys Asn Trp Phe His

180 185 190

Ile Glu Pro Trp Ile Trp Leu Met Asn Lys Thr Gln Asn Asn Leu Thr

195 200 205

Glu Gly Gln Pro Leu Arg Glu Cys Ala Val Thr Cys Arg Tyr Asp Lys

210 215 220

Glu Thr Glu Leu Asn Ile Val Thr Gln Ala Arg Asp Arg Pro Thr Thr

225 230 235 240

Leu Thr Gly Cys Lys Lys Gly Lys Asn Phe Ser Phe Ala Gly Val Ile

245 250 255

Leu Asp Gly Pro Cys Asn Phe Lys Val Ser Val Glu Asp Val Leu Phe

260 265 270

Lys Glu His Asp Cys Gly Asn Met Leu Gln Glu Thr Ala Ile Gln Leu

275 280 285

Leu Asp Gly Ala Thr Asn Thr Ile Glu Gly Ala Arg Val Gly Thr Ala

290 295 300

Lys Leu Thr Thr Trp Leu Gly Lys Gln Leu Gly Ile Leu Gly Lys Lys

305 310 315 320

Leu Glu Asn Lys Ser Lys Ala Trp Phe Gly Ala His Ala Ala Ser Pro

325 330 335

Tyr Cys Gly Val Glu Arg Lys Ile Gly Tyr Val Trp Tyr Thr Lys Asn

340 345 350

Cys Thr Pro Ala Cys Leu Pro Arg Asn Thr Arg Ile Ile Gly Pro Gly

355 360 365

Lys Phe Asp Thr Asn Ala Glu Asp Gly Lys Ile Leu His Glu Met Gly

370 375 380

Gly His Leu Ser Glu Phe Val Leu Leu Ser Leu Val Val Leu Ser Asp

385 390 395 400

Phe Ala Pro Glu Thr Ala Ser Val Ile Tyr Leu Val Leu His Phe Ala

405 410 415

Ile Pro Gln Ser His Val Asp Val Asp Thr Cys Asp Lys Asn Gln Leu

420 425 430

Asn Leu Thr Val Ala Thr Thr Val Ala Glu Val Ile Pro Gly Thr Val

435 440 445

Trp Asn Leu Gly Lys Tyr Val Cys Ile Arg Pro Asp Trp Trp Pro Tyr

450 455 460

Glu Thr Thr Thr Val Phe Val Ile Glu Glu Ala Gly Gln Val Ile Lys

465 470 475 480

Leu Met Leu Arg Ala Ile Arg Asp Leu Thr Arg Ile Trp Asn Ala Ala

485 490 495

Thr Thr Thr Ala Phe Leu Ile Phe Leu Val Lys Ala Leu Arg Gly Gln

500 505 510

Leu Ile Gln Gly Leu Leu Trp Leu Met Leu Ile Thr Gly Ala Gln Gly

515 520 525

Phe Pro Glu Cys Lys Glu Gly Phe Gln Tyr Ala Ile Ser Lys Asp Arg

530 535 540

Lys Met Gly Leu Leu Gly Pro Glu Ser Leu Thr Thr Thr Trp His Leu

545 550 555 560

Pro Thr Lys Lys Ile Val Asp Ser Met Val His Val Trp Cys Glu Gly

565 570 575

Lys Asp Leu Lys Ile Leu Lys Met Cys Thr Lys Glu Glu Arg Tyr Leu

580 585 590

Val Ala Val His Glu Arg Ala Leu Ser Thr Ser Ala Glu Phe Met Gln

595 600 605

Ile Ser Asp Gly Thr Ile Gly Pro Asp Val Ile Asp Met Pro Asp Asp

610 615 620

Phe Glu Phe Gly Leu Cys Pro Cys Asp Ser Lys Pro Val Ile Lys Gly

625 630 635 640

Lys Phe Asn Ala Ser Leu Leu Asn Gly Pro Ala Phe Gln Met Val Cys

645 650 655

Pro Gln Gly Trp Thr Gly Thr Ile Glu Cys Thr Leu Ala Asn Gln Asp

660 665 670

Thr Leu Asp Thr Thr Val Ile Arg Thr Tyr Arg Arg Thr Thr Pro Phe

675 680 685

Gln Arg Arg Lys Trp Cys Thr Tyr Glu Lys Ile Ile Gly Glu Asp Ile

690 695 700

Tyr Glu Cys Ile Leu Gly Gly Asn Trp Thr Cys Ile Thr Gly Asp His

705 710 715 720

Ser Arg Leu Lys Asp Gly Pro Ile Lys Lys Cys Lys Trp Cys Gly His

725 730 735

Asp Phe Val Asn Ser Glu Gly Leu Pro His Tyr Pro Ile Gly Lys Cys

740 745 750

Met Leu Ile Asn Glu Ser Gly Tyr Arg Tyr Val Asp Asp Thr Ser Cys

755 760 765

Asp Arg Gly Gly Val Ala Ile Val Pro Ser Gly Thr Val Lys Cys Arg

770 775 780

Ile Gly Asn Val Thr Val Gln Val Ile Ala Thr Asn Asn Asp Leu Gly

785 790 795 800

Pro Met Pro Cys Ser Pro Ala Glu Val Ile Ala Ser Glu Gly Pro Val

805 810 815

Glu Lys Thr Ala Cys Thr Phe Asn Tyr Ser Arg Thr Leu Pro Asn Lys

820 825 830

Tyr Tyr Glu Pro Arg Asp Arg Tyr Phe Gln Gln Tyr Met Leu Lys Gly

835 840 845

Glu Trp Gln Tyr Trp Phe Asp Leu Asp Ser Val Asp His His Lys Asp

850 855 860

Tyr Phe Ser Glu Phe Ile Ile Ile Ala Val Val Ala Leu Leu Gly Gly

865 870 875 880

Lys Tyr Val Leu Trp Leu Leu Ile Thr Tyr Thr Ile Leu Ser Glu Gln

885 890 895

Met Ala Met Gly Ala Gly Val Asn Thr Glu Glu Ile Val Met Ile Gly

900 905 910

Asn Leu Leu Thr Asp Ser Asp Ile Glu Val Val Val Tyr Phe Leu Leu

915 920 925

Leu Tyr Leu Ile Val Lys Glu Glu Leu Ala Arg Lys Trp Ile Ile Leu

930 935 940

Val Tyr His Ile Leu Val Ala Asn Pro Met Lys Thr Ile Gly Val Val

945 950 955 960

Leu Leu Met Leu Gly Gly Val Val Lys Ala Ser Arg Ile Asn Ala Asp

965 970 975

Asp Gln Ser Ala Met Asp Pro Cys Phe Leu Leu Val Thr Gly Val Val

980 985 990

Ala Val Leu Met Ile Ala Arg Arg Glu Pro Ala Thr Leu Pro Leu Ile

995 1000 1005

Val Ala Leu Leu Ala Ile Arg Thr Ser Gly Phe Leu Leu Pro Ala

1010 1015 1020

Ser Ile Asp Val Thr Val Ala Val Val Leu Ile Val Leu Leu Leu

1025 1030 1035

Ala Ser Tyr Ile Thr Asp Tyr Phe Arg Tyr Lys Lys Trp Leu Gln

1040 1045 1050

Leu Leu Phe Ser Leu Ile Ala Gly Ile Phe Ile Ile Arg Ser Leu

1055 1060 1065

Lys His Ile Asn Gln Met Glu Val Pro Glu Ile Ser Met Pro Ser

1070 1075 1080

Trp Arg Pro Leu Ala Leu Val Leu Phe Tyr Ile Thr Ser Thr Ala

1085 1090 1095

Ile Thr Thr Asn Trp Asp Ile Asp Leu Ala Gly Phe Leu Leu Gln

1100 1105 1110

Trp Ala Pro Ala Val Ile Met Met Ala Thr Met Trp Ala Asp Phe

1115 1120 1125

Leu Thr Leu Ile Ile Val Leu Pro Ser Tyr Glu Leu Ser Lys Leu

1130 1135 1140

Tyr Phe Leu Lys Asn Val Arg Thr Asp Val Glu Lys Asn Trp Leu

1145 1150 1155

Gly Lys Val Lys Tyr Arg Gln Ile Ser Ser Val Tyr Asp Ile Cys

1160 1165 1170

Asp Ser Glu Glu Ala Val Tyr Leu Phe Pro Ser Arg His Lys Ser

1175 1180 1185

Gly Ser Arg Pro Asp Phe Ile Leu Pro Phe Leu Lys Ala Val Leu

1190 1195 1200

Ile Ser Cys Ile Ser Ser Gln Trp Gln Val Val Tyr Ile Ser Tyr

1205 1210 1215

Leu Ile Leu Glu Ile Thr Tyr Tyr Met His Arg Lys Ile Ile Asp

1220 1225 1230

Glu Val Ser Gly Gly Ala Asn Phe Leu Ser Arg Leu Ile Ala Ala

1235 1240 1245

Ile Ile Glu Leu Asn Trp Ala Ile Asp Asp Glu Glu Cys Lys Gly

1250 1255 1260

Leu Lys Lys Leu Tyr Leu Leu Ser Gly Arg Ala Lys Asn Leu Ile

1265 1270 1275

Val Lys His Lys Val Arg Asn Glu Ala Val His Arg Trp Phe Gly

1280 1285 1290

Glu Glu Glu Ile Tyr Gly Ala Pro Lys Val Ile Thr Ile Ile Lys

1295 1300 1305

Ala Ser Thr Leu Ser Lys Asn Arg His Cys Ile Ile Cys Thr Ile

1310 1315 1320

Cys Glu Gly Lys Glu Trp Asn Gly Ala Asn Cys Pro Lys Cys Gly

1325 1330 1335

Arg Gln Gly Lys Pro Ile Thr Cys Gly Met Thr Leu Ala Asp Phe

1340 1345 1350

Glu Glu Lys His Tyr Lys Lys Ile Phe Ile Arg Glu Glu Ser Ser

1355 1360 1365

Cys Pro Val Pro Phe Asp Pro Ser Cys His Cys Asn Tyr Phe Arg

1370 1375 1380

His Asp Gly Pro Phe Arg Lys Glu Tyr Lys Gly Tyr Val Gln Tyr

1385 1390 1395

Thr Ala Arg Gly Gln Leu Phe Leu Arg Asn Leu Pro Ile Leu Ala

1400 1405 1410

Thr Lys Met Lys Leu Leu Met Val Gly Asn Leu Gly Ala Glu Ile

1415 1420 1425

Gly Asp Leu Glu His Leu Gly Trp Val Leu Arg Gly Pro Ala Val

1430 1435 1440

Cys Lys Lys Ile Thr Asn His Glu Lys Cys His Val Asn Ile Met

1445 1450 1455

Asp Lys Leu Thr Ala Phe Phe Gly Ile Met Pro Arg Gly Thr Thr

1460 1465 1470

Pro Arg Ala Pro Val Arg Phe Pro Thr Ala Leu Leu Lys Val Arg

1475 1480 1485

Arg Gly Leu Glu Thr Gly Trp Ala Tyr Thr His Gln Gly Gly Ile

1490 1495 1500

Ser Ser Val Asp His Val Thr Ala Gly Lys Asp Leu Leu Val Cys

1505 1510 1515

Asp Ser Met Gly Arg Thr Arg Val Val Cys His Ser Asn Asn Lys

1520 1525 1530

Met Thr Asp Glu Thr Glu Tyr Gly Ile Lys Thr Asp Ser Gly Cys

1535 1540 1545

Pro Glu Gly Ala Arg Cys Tyr Val Leu Asn Pro Glu Ala Val Asn

1550 1555 1560

Ile Ser Gly Thr Lys Gly Ala Met Val His Leu Gln Lys Thr Gly

1565 1570 1575

Gly Glu Phe Thr Cys Val Thr Ala Ser Gly Thr Pro Ala Phe Phe

1580 1585 1590

Asp Leu Lys Asn Leu Lys Gly Trp Ser Gly Leu Pro Ile Phe Glu

1595 1600 1605

Ala Ser Ser Gly Arg Val Val Gly Arg Val Lys Val Gly Lys Asn

1610 1615 1620

Glu Asp Ser Lys Pro Thr Lys Leu Met Ser Gly Ile Gln Thr Val

1625 1630 1635

Ser Lys Asn Gln Thr Asp Leu Ala Asp Ile Val Lys Lys Leu Thr

1640 1645 1650

Ser Met Asn Arg Gly Glu Phe Lys Gln Ile Thr Leu Ala Thr Gly

1655 1660 1665

Ala Gly Lys Thr Thr Glu Leu Pro Arg Ser Val Ile Glu Glu Ile

1670 1675 1680

Gly Arg His Lys Arg Val Leu Val Leu Ile Pro Leu Arg Ala Ala

1685 1690 1695

Ala Glu Ser Val Tyr Gln Tyr Met Arg Val Lys Tyr Pro Ser Ile

1700 1705 1710

Ser Phe Asn Leu Arg Ile Gly Asp Met Lys Glu Gly Asp Met Ala

1715 1720 1725

Thr Gly Ile Thr Tyr Ala Ser Tyr Gly Tyr Phe Cys Gln Leu Pro

1730 1735 1740

Gln Pro Lys Leu Arg Ala Ala Met Val Glu Tyr Ser Tyr Ile Phe

1745 1750 1755

Leu Asp Glu Tyr His Cys Ala Thr Pro Glu Gln Leu Ala Ile Ile

1760 1765 1770

Gly Lys Ile His Arg Phe Ala Glu Asn Leu Arg Val Val Ala Met

1775 1780 1785

Thr Ala Thr Pro Ala Gly Thr Val Thr Thr Thr Gly Gln Lys His

1790 1795 1800

Pro Ile Glu Glu Phe Ile Ala Pro Glu Val Met Lys Gly Glu Asp

1805 1810 1815

Leu Gly Ser Glu Tyr Leu Asp Ile Ala Gly Leu Lys Ile Pro Thr

1820 1825 1830

Glu Glu Met Lys Gly Asn Met Leu Val Phe Ala Pro Thr Arg Asn

1835 1840 1845

Met Ala Val Glu Thr Ala Lys Lys Leu Lys Ala Lys Gly Tyr Asn

1850 1855 1860

Ser Gly Tyr Tyr Tyr Ser Gly Glu Asn Pro Glu Asn Leu Arg Val

1865 1870 1875

Val Thr Ser Gln Ser Pro Tyr Val Val Val Ala Thr Asn Ala Ile

1880 1885 1890

Glu Ser Gly Val Thr Leu Pro Asp Leu Asp Thr Val Val Asp Thr

1895 1900 1905

Gly Leu Lys Cys Glu Lys Arg Val Arg Ile Ser Ser Lys Met Pro

1910 1915 1920

Phe Ile Val Thr Gly Leu Lys Arg Met Ala Val Thr Ile Gly Glu

1925 1930 1935

Gln Ala Gln Arg Arg Gly Arg Val Gly Arg Val Lys Pro Gly Arg

1940 1945 1950

Tyr Tyr Arg Ser Gln Glu Thr Ala Ser Gly Ser Lys Asp Tyr His

1955 1960 1965

Tyr Asp Leu Leu Gln Ala Gln Arg Tyr Gly Ile Glu Asp Gly Ile

1970 1975 1980

Asn Val Thr Lys Ser Phe Arg Glu Met Asn Tyr Asp Trp Ser Leu

1985 1990 1995

Tyr Glu Glu Asp Ser Leu Met Ile Thr Gln Leu Glu Val Leu Asn

2000 2005 2010

Asn Leu Leu Ile Ser Glu Asp Leu Pro Ala Ala Val Lys Asn Ile

2015 2020 2025

Met Ala Arg Thr Asp His Pro Glu Pro Ile Gln Leu Ala Tyr Asn

2030 2035 2040

Ser Tyr Glu Asn Gln Ile Pro Val Leu Phe Pro Lys Ile Lys Asn

2045 2050 2055

Gly Glu Val Thr Asp Ser Tyr Glu Asn Tyr Thr Tyr Leu Asn Ala

2060 2065 2070

Arg Lys Leu Gly Glu Asp Val Pro Ala Tyr Val Tyr Ala Thr Glu

2075 2080 2085

Asp Glu Asp Leu Ala Val Asp Leu Leu Gly Met Asp Trp Pro Asp

2090 2095 2100

Pro Gly Asn Gln Gln Val Val Glu Thr Gly Arg Ala Leu Lys Gln

2105 2110 2115

Val Thr Gly Leu Ser Thr Ala Glu Asn Ala Leu Leu Ile Ala Leu

2120 2125 2130

Phe Gly Tyr Val Gly Tyr Gln Thr Leu Ser Lys Arg His Ile Pro

2135 2140 2145

Met Ile Thr Asp Ile Tyr Thr Leu Glu Asp His Arg Leu Glu Asp

2150 2155 2160

Thr Thr His Leu Gln Phe Ala Pro Asn Ala Ile Arg Thr Asp Gly

2165 2170 2175

Lys Asp Ser Glu Leu Lys Glu Leu Ala Val Gly Asp Leu Asp Lys

2180 2185 2190

Tyr Val Asp Ala Leu Val Asp Tyr Ser Lys Gln Gly Met Lys Phe

2195 2200 2205

Ile Lys Val Gln Ala Glu Lys Val Arg Asp Ser Gln Ser Thr Lys

2210 2215 2220

Glu Gly Leu Gln Thr Ile Lys Glu Tyr Val Asp Lys Phe Ile Gln

2225 2230 2235

Ser Leu Thr Glu Asn Lys Glu Glu Ile Ile Arg Tyr Gly Leu Trp

2240 2245 2250

Gly Val His Thr Ala Leu Tyr Lys Ser Leu Ala Ala Arg Leu Gly

2255 2260 2265

His Glu Thr Ala Phe Ala Thr Leu Val Val Lys Trp Leu Ala Phe

2270 2275 2280

Gly Gly Glu Thr Val Ser Ala His Ile Lys Gln Val Ala Val Asp

2285 2290 2295

Leu Val Val Tyr Tyr Ile Ile Asn Lys Pro Ser Phe Pro Gly Asp

2300 2305 2310

Thr Glu Thr Gln Gln Glu Gly Arg Arg Phe Val Ala Ser Leu Phe

2315 2320 2325

Ile Ser Ala Leu Ala Thr Tyr Thr Tyr Lys Thr Trp Asn Tyr Asn

2330 2335 2340

Asn Leu Gln Arg Val Val Glu Pro Ala Leu Ala Tyr Leu Pro Tyr

2345 2350 2355

Ala Thr Ser Ala Leu Lys Leu Phe Thr Pro Thr Arg Leu Glu Ser

2360 2365 2370

Val Val Ile Leu Ser Ser Thr Ile Tyr Lys Thr Tyr Leu Ser Ile

2375 2380 2385

Arg Lys Gly Lys Ser Asp Gly Leu Leu Gly Thr Gly Ile Ser Ala

2390 2395 2400

Ala Met Glu Ile Leu Asn Gln Asn Pro Ile Ser Val Gly Ile Ser

2405 2410 2415

Val Met Leu Gly Val Gly Ala Ile Ala Ala His Asn Ala Ile Glu

2420 2425 2430

Ser Ser Glu Gln Lys Arg Thr Leu Leu Met Lys Val Phe Val Lys

2435 2440 2445

Asn Phe Leu Asp Gln Ala Ala Thr Asp Glu Leu Val Lys Glu Asn

2450 2455 2460

Pro Glu Lys Ile Ile Met Ala Leu Phe Glu Ala Val Gln Thr Ile

2465 2470 2475

Gly Asn Pro Leu Arg Leu Ile Tyr His Leu Tyr Gly Val Tyr Tyr

2480 2485 2490

Lys Gly Trp Glu Ala Lys Glu Leu Ala Glu Lys Thr Ala Gly Arg

2495 2500 2505

Asn Leu Phe Thr Leu Ile Met Phe Glu Ala Phe Glu Leu Leu Gly

2510 2515 2520

Met Asp Ser Glu Gly Lys Ile Arg Asn Leu Ser Gly Asn Tyr Ile

2525 2530 2535

Leu Asp Leu Ile Phe Asn Leu His Asn Lys Leu Asn Lys Gly Leu

2540 2545 2550

Lys Lys Leu Val Leu Gly Trp Ala Pro Ala Pro Leu Ser Cys Asp

2555 2560 2565

Trp Thr Pro Ser Asp Glu Arg Ile Ser Leu Pro His Asn Asn Tyr

2570 2575 2580

Leu Arg Val Glu Thr Arg Cys Pro Cys Gly Tyr Glu Met Lys Ala

2585 2590 2595

Ile Lys Asn Val Ala Gly Lys Leu Thr Lys Val Glu Glu Lys Gly

2600 2605 2610

Ser Phe Leu Cys Arg Asn Arg Leu Gly Arg Gly Pro Pro Asn Phe

2615 2620 2625

Lys Val Thr Lys Phe Tyr Asp Asp Asn Leu Ile Glu Val Lys Pro

2630 2635 2640

Val Ala Arg Leu Glu Gly Gln Val Asp Leu Tyr Tyr Lys Gly Val

2645 2650 2655

Thr Ala Lys Leu Asp Tyr Asn Asn Gly Lys Val Leu Leu Ala Thr

2660 2665 2670

Asn Lys Trp Glu Val Asp His Ala Phe Leu Thr Arg Leu Val Lys

2675 2680 2685

Lys His Thr Gly Ile Gly Phe Lys Gly Ala Tyr Leu Gly Asp Arg

2690 2695 2700

Pro Asp His Gln Asp Leu Val Asp Arg Asp Cys Ala Thr Ile Thr

2705 2710 2715

Lys Asn Ser Val Gln Phe Leu Lys Met Lys Lys Gly Cys Ala Phe

2720 2725 2730

Thr Tyr Asp Leu Thr Ile Ser Asn Leu Val Arg Leu Ile Glu Leu

2735 2740 2745

Val His Lys Asn Asn Leu Gln Glu Arg Glu Ile Pro Thr Val Thr

2750 2755 2760

Val Thr Thr Trp Leu Ala Tyr Ser Phe Val Asn Glu Asp Leu Gly

2765 2770 2775

Thr Ile Lys Pro Val Leu Gly Glu Lys Val Ile Pro Glu Pro Pro

2780 2785 2790

Glu Glu Leu Ser Leu Gln Pro Thr Val Arg Leu Val Thr Thr Glu

2795 2800 2805

Thr Ala Ile Thr Ile Thr Gly Glu Ala Glu Val Met Thr Thr Gly

2810 2815 2820

Ile Thr Pro Val Val Glu Met Lys Glu Glu Pro Gln Leu Asp His

2825 2830 2835

Gln Ser Thr Thr Leu Lys Val Gly Leu Lys Glu Gly Glu Tyr Pro

2840 2845 2850

Gly Pro Gly Val Asn Pro Asn His Leu Ala Glu Val Ile Asp Glu

2855 2860 2865

Lys Asp Asp Arg Pro Phe yal Leu Ile Ile Gly Asn Lys Gly Ser

2870 2875 2880

Thr Ser Asn Arg Ala Arg Thr Ala Lys Asn Ile Arg Leu Tyr Lys

2885 2890 2895

Gly Asn Asn Pro Arg Glu Ile Arg Asp Leu Met Ser Gln Gly Arg

2900 2905 2910

Ile Leu Thr Val Ala Leu Lys Glu Leu Asp Pro Glu Leu Lys Glu

2915 2920 2925

Leu Val Asp Tyr Lys Gly Thr Phe Leu Asn Arg Glu Ala Leu Glu

2930 2935 2940

Ala Leu Ser Leu Gly Lys Pro Ile Lys Arg Lys Thr Thr Thr Ala

2945 2950 2955

Met Ile Arg Arg Leu Ile Glu Pro Glu Val Glu Glu Glu Leu Pro

2960 2965 2970

Asp Trp Phe Gln Ala Glu Glu Pro Leu Phe Leu Glu Ala Lys Ile

2975 2980 2985

Gln Asn Asp Leu Tyr His Leu Ile Gly Ser Val Asp Ser Ile Lys

2990 2995 3000

Ser Lys Ala Lys Glu Leu Gly Ala Thr Asp Asn Thr Lys Ile Val

3005 3010 3015

Lys Glu Val Gly Ala Arg Thr Tyr Thr Met Lys Leu Ser Ser Trp

3020 3025 3030

Ser Thr Gln Val Thr Lys Lys Gln Met Ser Leu Ala Pro Leu Phe

3035 3040 3045

Glu Glu Leu Leu Leu Lys Cys Pro Pro Cys Ser Lys Ile Ser Lys

3050 3055 3060

Gly His Met Val Ser Ala Tyr Gln Leu Ala Gln Gly Asn Trp Glu

3065 3070 3075

Pro Leu Gly Cys Gly Val Tyr Met Gly Thr Ile Pro Ala Arg Arg

3080 3085 3090

Leu Lys Ile His Pro Tyr Glu Ala Tyr Leu Lys Leu Lys Glu Leu

3095 3100 3105

Val Glu Val Glu Ser Ser Arg Ala Thr Ala Lys Glu Ser Ile Ile

3110 3115 3120

Arg Glu His Asn Thr Trp Ile Leu Arg Lys Val Arg His Glu Gly

3125 3130 3135

Asn Leu Arg Thr Lys Ser Met Ile Asn Pro Gly Lys Ile Ser Asp

3140 3145 3150

Gln Leu Cys Arg Asp Gly His Lys Arg Asn Ile Tyr Asn Lys Ile

3155 3160 3165

Ile Gly Ser Thr Met Ala Ser Ala Gly Ile Arg Leu Glu Lys Leu

3170 3175 3180

Pro Val Val Arg Ala Gln Thr Asp Thr Thr Ser Phe His Gln Ala

3185 3190 3195

Ile Arg Glu Lys Ile Asp Lys Thr Glu Asn Lys Gln Thr Pro Glu

3200 3205 3210

Leu His Glu Glu Leu Met Lys Val Phe Asp Cys Leu Lys Ile Pro

3215 3220 3225

Glu Leu Lys Glu Ser Tyr Asp Glu Val Ser Trp Glu Gln Leu Glu

3230 3235 3240

Ala Gly Ile Asn Arg Lys Gly Ala Ala Gly Tyr Leu Glu Ser Lys

3245 3250 3255

Asn Ile Gly Glu Val Leu Asp Thr Glu Lys His Ile Val Glu Gln

3260 3265 3270

Leu Ile Lys Asp Leu Arg Lys Gly Lys Lys Ile Arg Tyr Tyr Glu

3275 3280 3285

Thr Ala Ile Pro Lys Asn Glu Lys Arg Asp Val Ser Asp Asp Trp

3290 3295 3300

Glu Ala Gly Glu Phe Val Asp Glu Lys Lys Pro Arg Val Ile Gln

3305 3310 3315

Tyr Pro Asp Ala Lys Val Arg Leu Ala Ile Thr Lys Val Met Tyr

3320 3325 3330

Lys Trp Val Lys Gln Lys Pro Val Val Ile Pro Gly Tyr Glu Gly

3335 3340 3345

Lys Thr Pro Leu Phe Asp Ile Phe Asn Lys Val Lys Lys Glu Trp

3350 3355 3360

Asp Ser Phe Gln Asp Pro Val Ala Val Ser Phe Asp Thr Lys Ala

3365 3370 3375

Trp Asp Thr Gln Val Thr Ser Arg Asp Leu Met Leu Ile Lys Asp

3380 3385 3390

Ile Gln Lys Tyr Tyr Phe Lys Arg Ser Ile His Lys Phe Leu Asp

3395 3400 3405

Thr Ile Thr Glu His Met Val Glu Val Pro Val Ile Thr Ala Asp

3410 3415 3420

Gly Glu Val Tyr Ile Arg Asn Gly Gln Arg Gly Ser Gly Gln Pro

3425 3430 3435

Asp Thr Ser Ala Gly Asn Ser Met Leu Asn Val Leu Thr Met Ile

3440 3445 3450

Tyr Ala Phe Cys Lys Ser Thr Gly Ile Pro Tyr Arg Gly Phe Ser

3455 3460 3465

Arg Val Ala Arg Ile His Val Cys Gly Asp Asp Gly Phe Leu Ile

3470 3475 3480

Thr Glu Arg Gly Leu Gly Leu Lys Phe Ser Glu Lys Gly Met Gln

3485 3490 3495

Ile Leu His Glu Ala Gly Lys Pro Gln Lys Ile Thr Glu Gly Asp

3500 3505 3510

Lys Met Lys Val Ala Tyr Arg Phe Glu Asp Ile Glu Phe Cys Ser

3515 3520 3525

His Thr Pro Val Pro Val Arg Trp Ala Asp Asn Thr Ser Ser Tyr

3530 3535 3540

Met Ala Gly Arg Ser Thr Ala Thr Ile Leu Ala Lys Met Ala Thr

3545 3550 3555

Arg Leu Asp Ser Ser Gly Glu Arg Gly Ser Thr Ala Tyr Glu Lys

3560 3565 3570

Ala Val Ala Phe Ser Phe Leu Leu Met Tyr Ser Trp Asn Pro Val

3575 3580 3585

Val Arg Arg Ile Cys Leu Leu Val Leu Ser Gln Phe Pro Glu Ile

3590 3595 3600

Ser Pro Ser Lys Asn Thr Ile Tyr Tyr Tyr Gln Gly Asp Pro Ile

3605 3610 3615

Ala Ala Tyr Arg Glu Val Ile Gly Lys Gln Leu Cys Glu Leu Lys

3620 3625 3630

Arg Thr Gly Phe Glu Lys Leu Ala Gly Leu Asn Leu Ser Met Thr

3635 3640 3645

Thr Leu Gly Ile Trp Thr Lys His Thr Ser Lys Arg Leu Ile Gln

3650 3655 3660

Ala Cys Val Glu Ile Gly Lys Arg Glu Gly Thr Trp Leu Val Asn

3665 3670 3675

Ala Asp Arg Leu Ile Ala Gly Lys Thr Gly Lys Phe Tyr Ile Pro

3680 3685 3690

Ser Thr Gly Val Thr Leu Leu Gly Lys His Tyr Glu Glu Ile Asn

3695 3700 3705

Leu Lys Gln Lys Ala Ala Gln Pro Pro Ile Glu Gly Val Asp Arg

3710 3715 3720

Tyr Lys Leu Gly Pro Ile Val Asn Val Ile Leu Arg Arg Leu Arg

3725 3730 3735

Val Met Leu Met Thr Val Ala Ser Gly Ser Trp

3740 3745

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Met Glu Leu Phe Ser Asn Glu Leu Leu Tyr Lys Thr Tyr Lys Gln Lys

1 5 10 15

Pro Ala Gly Val Val Glu Pro Val Tyr Asp Val Asn Gly Arg Pro Leu

20 25 30

Phe Gly Glu Ser Ser Asp Leu His Pro Gln Ser Thr Leu Lys Leu Pro

35 40 45

His Gln Arg Gly Ser Ala Asn Ile Leu Thr Asn Ala Arg Ser Leu Pro

50 55 60

Arg Lys Gly Asp Cys Arg Arg Gly Asn Val Tyr Gly Pro Val Ser Gly

65 70 75 80

Ile Tyr Ile Lys Pro Gly Pro Ile Tyr Tyr Gln Asp Tyr Val Gly Pro

85 90 95

Val Tyr His Arg Ala Pro Leu Glu Leu Cys Arg Glu Ala Ser Met Cys

100 105 110

Glu Thr Thr Arg Arg Val Gly Arg Val Thr Gly Ser Asp Gly Lys Leu

115 120 125

Tyr His Ile Tyr Ile Cys Ile Asp Gly Cys Ile Leu Leu Lys Arg Ala

130 135 140

Thr Arg Asn Gln Pro Glu Val Leu Lys Trp Val Tyr Asn Arg Leu Asn

145 150 155 160

Cys Pro Leu Trp Val Thr Ser Cys Ser Asp Glu Gly Ser Lys Gly Ala

165 170 175

Thr Ser Lys Lys Gln Pro Lys Pro Asp Arg Ile Glu Lys Gly Lys Met

180 185 190

Lys Ile Ala Pro Lys Glu Thr Glu Lys Asp Cys Lys Thr Arg Pro Pro

195 200 205

Asp Ala Thr Ile Val Val Glu Gly Val Lys Tyr Gln Val Lys Lys Lys

210 215 220

Gly Lys Val Arg Gly Lys Asn Thr Gln Asp Gly Leu Tyr His Asn Lys

225 230 235 240

Asn Lys Pro Pro Glu Ser Arg Lys Lys Leu Glu Lys Ala Leu Leu Ala

245 250 255

Trp Ala Ile Leu Ala Ala Val Leu Leu Gln Leu Val Thr Gly Glu Asn

260 265 270

Ile Thr Gln Trp Asn Leu Met Asp Asn Gly Thr Glu Gly Ile Gln Gln

275 280 285

Ala Met Phe Leu Arg Gly Val Asn Arg Ser Leu His Gly Ile Trp Pro

290 295 300

Glu Lys Ile Cys Thr Gly Val Pro Thr His Leu Ala Thr Asp Tyr Glu

305 310 315 320

Leu Lys Glu Ile Val Gly Met Met Asp Ala Ser Glu Lys Thr Asn Tyr

325 330 335

Thr Cys Cys Arg Leu Gln Arg His Glu Trp Asn Lys Gly Trp Cys Asn

340 345 350

Trp Phe His Ile Glu Pro Trp Ile Trp Leu Met Asn Lys Thr Gln Asn

355 360 365

Asn Leu Thr Glu Gly Gln Pro Leu Arg Glu Cys Ala Val Thr Cys Arg

370 375 380

Tyr Asp Lys Glu Thr Glu Leu Asn Ile Val Thr Gln Ala Arg Asp Arg

385 390 395 400

Pro Thr Thr Leu Thr Gly Cys Lys Lys Gly Lys Asn Phe Ser Phe Ala

405 410 415

Gly Val Ile Leu Asp Gly Pro Cys Asn Phe Lys Val Ser Val Glu Asp

420 425 430

Val Leu Phe Lys Glu His Asp Cys Gly Asn Met Leu Gln Glu Thr Ala

435 440 445

Ile Gln Leu Leu Asp Gly Ala Thr Asn Thr Ile Glu Gly Ala Arg Val

450 455 460

Gly Thr Ala Lys Leu Thr Thr Trp Leu Gly Lys Gln Leu Gly Ile Leu

465 470 475 480

Gly Lys Lys Leu Glu Asn Lys Ser Lys Ala Trp Phe Gly Ala His Ala

485 490 495

Ala Ser Pro Tyr Cys Gly Val Glu Arg Lys Ile Gly Tyr Val Trp Tyr

500 505 510

Thr Lys Asn Cys Thr Pro Ala Cys Leu Pro Arg Asn Thr Arg Ile Ile

515 520 525

Gly Pro Gly Lys Phe Asp Thr Asn Ala Glu Asp Gly Lys Ile Leu His

530 535 540

Glu Met Gly Gly His Leu Ser Glu Phe Val Leu Leu Ser Leu Val Val

545 550 555 560

Leu Ser Asp Phe Ala Pro Glu Thr Ala Ser Val Ile Tyr Leu Val Leu

565 570 575

His Phe Ala Ile Pro Gln Ser His Val Asp Val Asp Thr Cys Asp Lys

580 585 590

Asn Gln Leu Asn Leu Thr Val Ala Thr Thr Val Ala Glu Val Ile Pro

595 600 605

Gly Thr Val Trp Asn Leu Gly Lys Tyr Val Cys Ile Arg Pro Asp Trp

610 615 620

Trp Pro Tyr Glu Thr Thr Thr Val Phe Val Ile Glu Glu Ala Gly Gln

625 630 635 640

Val Ile Lys Leu Met Leu Arg Ala Ile Arg Asp Leu Thr Arg Ile Trp

645 650 655

Asn Ala Ala Thr Thr Thr Ala Phe Leu Ile Phe Leu Val Lys Ala Leu

660 665 670

Arg Gly Gln Leu Ile Gln Gly Leu Leu Trp Leu Met Leu Ile Thr Gly

675 680 685

Ala Gln Gly Phe Pro Glu Cys Lys Glu Gly Phe Gln Tyr Ala Ile Ser

690 695 700

Lys Asp Arg Lys Met Gly Leu Leu Gly Pro Glu Ser Leu Thr Thr Thr

705 710 715 720

Trp His Leu Pro Thr Lys Lys Ile Val Asp Ser Met Val His Val Trp

725 730 735

Cys Glu Gly Lys Asp Leu Lys Ile Leu Lys Met Cys Thr Lys Glu Glu

740 745 750

Arg Tyr Leu Val Ala Val His Glu Arg Ala Leu Ser Thr Ser Ala Glu

755 760 765

Phe Met Gln Ile Ser Asp Gly Thr Ile Gly Pro Asp Val Ile Asp Met

770 775 780

Pro Asp Asp Phe Glu Phe Gly Leu Cys Pro Cys Asp Ser Lys Pro Val

785 790 795 800

Ile Lys Gly Lys Phe Asn Ala Ser Leu Leu Asn Gly Pro Ala Phe Gln

805 810 815

Met Val Cys Pro Gln Gly Trp Thr Gly Thr Ile Glu Cys Thr Leu Ala

820 825 830

Asn Gln Asp Thr Leu Asp Thr Thr Val Ile Arg Thr Tyr Arg Arg Thr

835 840 845

Thr Pro Phe Gln Arg Arg Lys Trp Cys Thr Tyr Glu Lys Ile Ile Gly

850 855 860

Glu Asp Ile Tyr Glu Cys Ile Leu Gly Gly Asn Trp Thr Cys Ile Thr

865 870 875 880

Gly Asp His Ser Arg Leu Lys Asp Gly Pro Ile Lys Lys Cys Lys Trp

885 890 895

Cys Gly His Asp Phe Val Asn Ser Glu Gly Leu Pro His Tyr Pro Ile

900 905 910

Gly Lys Cys Met Leu Ile Asn Glu Ser Gly Tyr Arg Tyr Val Asp Asp

915 920 925

Thr Ser Cys Asp Arg Gly Gly Val Ala Ile Val Pro Ser Gly Thr Val

930 935 940

Lys Cys Arg Ile Gly Asn Val Thr Val Gln Val Ile Ala Thr Asn Asn

945 950 955 960

Asp Leu Gly Pro Met Pro Cys Ser Pro Ala Glu Val Ile Ala Ser Glu

965 970 975

Gly Pro Val Glu Lys Thr Ala Cys Thr Phe Asn Tyr Ser Arg Thr Leu

980 985 990

Pro Asn Lys Tyr Tyr Glu Pro Arg Asp Arg Tyr Phe Gln Gln Tyr Met

995 1000 1005

Leu Lys Gly Glu Trp Gln Tyr Trp Phe Asp Leu Asp Ser Val Asp

1010 1015 1020

His His Lys Asp Tyr Phe Ser Glu Phe Ile Ile Ile Ala Val Val

1025 1030 1035

Ala Leu Leu Gly Gly Lys Tyr Val Leu Trp Leu Leu Ile Thr Tyr

1040 1045 1050

Thr Ile Leu Ser Glu Gln Met Ala Met Gly Ala Gly Val Asn Thr

1055 1060 1065

Glu Glu Ile Val Met Ile Gly Asn Leu Leu Thr Asp Ser Asp Ile

1070 1075 1080

Glu Val Val Val Tyr Phe Leu Leu Leu Tyr Leu Ile Val Lys Glu

1085 1090 1095

Glu Leu Ala Arg Lys Trp Ile Ile Leu Val Tyr His Ile Leu Val

1100 1105 1110

Ala Asn Pro Met Lys Thr Ile Gly Val Val Leu Leu Met Leu Gly

1115 1120 1125

Gly Val Val Lys Ala Ser Arg Ile Asn Ala Asp Asp Gln Ser Ala

1130 1135 1140

Met Asp Pro Cys Phe Leu Leu Val Thr Gly Val Val Ala Val Leu

1145 1150 1155

Met Ile Ala Arg Arg Glu Pro Ala Thr Leu Pro Leu Ile Val Ala

1160 1165 1170

Leu Leu Ala Ile Arg Thr Ser Gly Phe Leu Leu Pro Ala Ser Ile

1175 1180 1185

Asp Val Thr Val Ala Val Val Leu Ile Val Leu Leu Leu Ala Ser

1190 1195 1200

Tyr Ile Thr Asp Tyr Phe Arg Tyr Lys Lys Trp Leu Gln Leu Leu

1205 1210 1215

Phe Ser Leu Ile Ala Gly Ile Phe Ile Ile Arg Ser Leu Lys His

1220 1225 1230

Ile Asn Gln Met Glu Val Pro Glu Ile Ser Met Pro Ser Trp Arg

1235 1240 1245

Pro Leu Ala Leu Val Leu Phe Tyr Ile Thr Ser Thr Ala Ile Thr

1250 1255 1260

Thr Asn Trp Asp Ile Asp Leu Ala Gly Phe Leu Leu Gln Trp Ala

1265 1270 1275

Pro Ala Val Ile Met Met Ala Thr Met Trp Ala Asp Phe Leu Thr

1280 1285 1290

Leu Ile Ile Val Leu Pro Ser Tyr Glu Leu Ser Lys Leu Tyr Phe

1295 1300 1305

Leu Lys Asn Val Arg Thr Asp Val Glu Lys Asn Trp Leu Gly Lys

1310 1315 1320

Val Lys Tyr Arg Gln Ile Ser Ser Val Tyr Asp Ile Cys Asp Ser

1325 1330 1335

Glu Glu Ala Val Tyr Leu Phe Pro Ser Arg His Lys Ser Gly Ser

1340 1345 1350

Arg Pro Asp Phe Ile Leu Pro Phe Leu Lys Ala Val Leu Ile Ser

1355 1360 1365

Cys Ile Ser Ser Gln Trp Gln Val Val Tyr Ile Ser Tyr Leu Ile

1370 1375 1380

Leu Glu Ile Thr Tyr Tyr Met His Arg Lys Ile Ile Asp Glu Val

1385 1390 1395

Ser Gly Gly Ala Asn Phe Leu Ser Arg Leu Ile Ala Ala Ile Ile

1400 1405 1410

Glu Leu Asn Trp Ala Ile Asp Asp Glu Glu Cys Lys Gly Leu Lys

1415 1420 1425

Lys Leu Tyr Leu Leu Ser Gly Arg Ala Lys Asn Leu Ile Val Lys

1430 1435 1440

His Lys Val Arg Asn Glu Ala Val His Arg Trp Phe Gly Glu Glu

1445 1450 1455

Glu Ile Tyr Gly Ala Pro Lys Val Ile Thr Ile Ile Lys Ala Ser

1460 1465 1470

Thr Leu Ser Lys Asn Arg His Cys Ile Ile Cys Thr Ile Cys Glu

1475 1480 1485

Gly Lys Glu Trp Asn Gly Ala Asn Cys Pro Lys Cys Gly Arg Gln

1490 1495 1500

Gly Lys Pro Ile Thr Cys Gly Met Thr Leu Ala Asp Phe Glu Glu

1505 1510 1515

Lys His Tyr Lys Lys Ile Phe Ile Arg Glu Glu Ser Ser Cys Pro

1520 1525 1530

Val Pro Phe Asp Pro Ser Cys His Cys Asn Tyr Phe Arg His Asp

1535 1540 1545

Gly Pro Phe Arg Lys Glu Tyr Lys Gly Tyr Val Gln Tyr Thr Ala

1550 1555 1560

Arg Gly Gln Leu Phe Leu Arg Asn Leu Pro Ile Leu Ala Thr Lys

1565 1570 1575

Met Lys Leu Leu Met Val Gly Asn Leu Gly Ala Glu Ile Gly Asp

1580 1585 1590

Leu Glu His Leu Gly Trp Val Leu Arg Gly Pro Ala Val Cys Lys

1595 1600 1605

Lys Ile Thr Asn His Glu Lys Cys His Val Asn Ile Met Asp Lys

1610 1615 1620

Leu Thr Ala Phe Phe Gly Ile Met Pro Arg Gly Thr Thr Pro Arg

1625 1630 1635

Ala Pro Val Arg Phe Pro Thr Ala Leu Leu Lys Val Arg Arg Gly

1640 1645 1650

Leu Glu Thr Gly Trp Ala Tyr Thr His Gln Gly Gly Ile Ser Ser

1655 1660 1665

Val Asp His Val Thr Ala Gly Lys Asp Leu Leu Val Cys Asp Ser

1670 1675 1680

Met Gly Arg Thr Arg Val Val Cys His Ser Asn Asn Lys Met Thr

1685 1690 1695

Asp Glu Thr Glu Tyr Gly Ile Lys Thr Asp Ser Gly Cys Pro Glu

1700 1705 1710

Gly Ala Arg Cys Tyr Val Leu Asn Pro Glu Ala Val Asn Ile Ser

1715 1720 1725

Gly Thr Lys Gly Ala Met Val His Leu Gln Lys Thr Gly Gly Glu

1730 1735 1740

Phe Thr Cys Val Thr Ala Ser Gly Thr Pro Ala Phe Phe Asp Leu

1745 1750 1755

Lys Asn Leu Lys Gly Trp Ser Gly Leu Pro Ile Phe Glu Ala Ser

1760 1765 1770

Ser Gly Arg Val Val Gly Arg Val Lys Val Gly Lys Asn Glu Asp

1775 1780 1785

Ser Lys Pro Thr Lys Leu Met Ser Gly Ile Gln Thr Val Ser Lys

1790 1795 1800

Asn Gln Thr Asp Leu Ala Asp Ile Val Lys Lys Leu Thr Ser Met

1805 1810 1815

Asn Arg Gly Glu Phe Lys Gln Ile Thr Leu Ala Thr Gly Ala Gly

1820 1825 1830

Lys Thr Thr Glu Leu Pro Arg Ser Val Ile Glu Glu Ile Gly Arg

1835 1840 1845

His Lys Arg Val Leu Val Leu Ile Pro Leu Arg Ala Ala Ala Glu

1850 1855 1860

Ser Val Tyr Gln Tyr Met Arg Val Lys Tyr Pro Ser Ile Ser Phe

1865 1870 1875

Asn Leu Arg Ile Gly Asp Met Lys Glu Gly Asp Met Ala Thr Gly

1880 1885 1890

Ile Thr Tyr Ala Ser Tyr Gly Tyr Phe Cys Gln Leu Pro Gln Pro

1895 1900 1905

Lys Leu Arg Ala Ala Met Val Glu Tyr Ser Tyr Ile Phe Leu Asp

1910 1915 1920

Glu Tyr His Cys Ala Thr Pro Glu Gln Leu Ala Ile Ile Gly Lys

1925 1930 1935

Ile His Arg Phe Ala Glu Asn Leu Arg Val Val Ala Met Thr Ala

1940 1945 1950

Thr Pro Ala Gly Thr Val Thr Thr Thr Gly Gln Lys His Pro Ile

1955 1960 1965

Glu Glu Phe Ile Ala Pro Glu Val Met Lys Gly Glu Asp Leu Gly

1970 1975 1980

Ser Glu Tyr Leu Asp Ile Ala Gly Leu Lys Ile Pro Thr Glu Glu

1985 1990 1995

Met Lys Gly Asn Met Leu Val Phe Ala Pro Thr Arg Asn Met Ala

2000 2005 2010

Val Glu Thr Ala Lys Lys Leu Lys Ala Lys Gly Tyr Asn Ser Gly

2015 2020 2025

Tyr Tyr Tyr Ser Gly Glu Asn Pro Glu Asn Leu Arg Val Val Thr

2030 2035 2040

Ser Gln Ser Pro Tyr Val Val Val Ala Thr Asn Ala Ile Glu Ser

2045 2050 2055

Gly Val Thr Leu Pro Asp Leu Asp Thr Val Val Asp Thr Gly Leu

2060 2065 2070

Lys Cys Glu Lys Arg Val Arg Ile Ser Ser Lys Met Pro Phe Ile

2075 2080 2085

Val Thr Gly Leu Lys Arg Met Ala Val Thr Ile Gly Glu Gln Ala

2090 2095 2100

Gln Arg Arg Gly Arg Val Gly Arg Val Lys Pro Gly Arg Tyr Tyr

2105 2110 2115

Arg Ser Gln Glu Thr Ala Ser Gly Ser Lys Asp Tyr His Tyr Asp

2120 2125 2130

Leu Leu Gln Ala Gln Arg Tyr Gly Ile Glu Asp Gly Ile Asn Val

2135 2140 2145

Thr Lys Ser Phe Arg Glu Met Asn Tyr Asp Trp Ser Leu Tyr Glu

2150 2155 2160

Glu Asp Ser Leu Met Ile Thr Gln Leu Glu Val Leu Asn Asn Leu

2165 2170 2175

Leu Ile Ser Glu Asp Leu Pro Ala Ala Val Lys Asn Ile Met Ala

2180 2185 2190

Arg Thr Asp His Pro Glu Pro Ile Gln Leu Ala Tyr Asn Ser Tyr

2195 2200 2205

Glu Asn Gln Ile Pro Val Leu Phe Pro Lys Ile Lys Asn Gly Glu

2210 2215 2220

Val Thr Asp Ser Tyr Glu Asn Tyr Thr Tyr Leu Asn Ala Arg Lys

2225 2230 2235

Leu Gly Glu Asp Val Pro Ala Tyr Val Tyr Ala Thr Glu Asp Glu

2240 2245 2250

Asp Leu Ala Val Asp Leu Leu Gly Met Asp Trp Pro Asp Pro Gly

2255 2260 2265

Asn Gln Gln Val Val Glu Thr Gly Arg Ala Leu Lys Gln Val Thr

2270 2275 2280

Gly Leu Ser Thr Ala Glu Asn Ala Leu Leu Ile Ala Leu Phe Gly

2285 2290 2295

Tyr Val Gly Tyr Gln Thr Leu Ser Lys Arg His Ile Pro Met Ile

2300 2305 2310

Thr Asp Ile Tyr Thr Leu Glu Asp His Arg Leu Glu Asp Thr Thr

2315 2320 2325

His Leu Gln Phe Ala Pro Asn Ala Ile Arg Thr Asp Gly Lys Asp

2330 2335 2340

Ser Glu Leu Lys Glu Leu Ala Val Gly Asp Leu Asp Lys Tyr Val

2345 2350 2355

Asp Ala Leu Val Asp Tyr Ser Lys Gln Gly Met Lys Phe Ile Lys

2360 2365 2370

Val Gln Ala Glu Lys Val Arg Asp Ser Gln Ser Thr Lys Glu Gly

2375 2380 2385

Leu Gln Thr Ile Lys Glu Tyr Val Asp Lys Phe Ile Gln Ser Leu

2390 2395 2400

Thr Glu Asn Lys Glu Glu Ile Ile Arg Tyr Gly Leu Trp Gly Val

2405 2410 2415

His Thr Ala Leu Tyr Lys Ser Leu Ala Ala Arg Leu Gly His Glu

2420 2425 2430

Thr Ala Phe Ala Thr Leu Val Val Lys Trp Leu Ala Phe Gly Gly

2435 2440 2445

Glu Thr Val Ser Ala His Ile Lys Gln Val Ala Val Asp Leu Val

2450 2455 2460

Val Tyr Tyr Ile Ile Asn Lys Pro Ser Phe Pro Gly Asp Thr Glu

2465 2470 2475

Thr Gln Gln Glu Gly Arg Arg Phe Val Ala Ser Leu Phe Ile Ser

2480 2485 2490

Ala Leu Ala Thr Tyr Thr Tyr Lys Thr Trp Asn Tyr Asn Asn Leu

2495 2500 2505

Gln Arg Val Val Glu Pro Ala Leu Ala Tyr Leu Pro Tyr Ala Thr

2510 2515 2520

Ser Ala Leu Lys Leu Phe Thr Pro Thr Arg Leu Glu Ser Val Val

2525 2530 2535

Ile Leu Ser Ser Thr Ile Tyr Lys Thr Tyr Leu Ser Ile Arg Lys

2540 2545 2550

Gly Lys Ser Asp Gly Leu Leu Gly Thr Gly Ile Ser Ala Ala Met

2555 2560 2565

Glu Ile Leu Asn Gln Asn Pro Ile Ser Val Gly Ile Ser Val Met

2570 2575 2580

Leu Gly Val Gly Ala Ile Ala Ala His Asn Ala Ile Glu Ser Ser

2585 2590 2595

Glu Gln Lys Arg Thr Leu Leu Met Lys Val Phe Val Lys Asn Phe

2600 2605 2610

Leu Asp Gln Ala Ala Thr Asp Glu Leu Val Lys Glu Asn Pro Glu

2615 2620 2625

Lys Ile Ile Met Ala Leu Phe Glu Ala Val Gln Thr Ile Gly Asn

2630 2635 2640

Pro Leu Arg Leu Ile Tyr His Leu Tyr Gly Val Tyr Tyr Lys Gly

2645 2650 2655

Trp Glu Ala Lys Glu Leu Ala Glu Lys Thr Ala Gly Arg Asn Leu

2660 2665 2670

Phe Thr Leu Ile Met Phe Glu Ala Phe Glu Leu Leu Gly Met Asp

2675 2680 2685

Ser Glu Gly Lys Ile Arg Asn Leu Ser Gly Asn Tyr Ile Leu Asp

2690 2695 2700

Leu Ile Phe Asn Leu His Asn Lys Leu Asn Lys Gly Leu Lys Lys

2705 2710 2715

Leu Val Leu Gly Trp Ala Pro Ala Pro Leu Ser Cys Asp Trp Thr

2720 2725 2730

Pro Ser Asp Glu Arg Ile Ser Leu Pro His Asn Asn Tyr Leu Arg

2735 2740 2745

Val Glu Thr Arg Cys Pro Cys Gly Tyr Glu Met Lys Ala Ile Lys

2750 2755 2760

Asn Val Ala Gly Lys Leu Thr Lys Val Glu Glu Lys Gly Ser Phe

2765 2770 2775

Leu Cys Arg Asn Arg Leu Gly Arg Gly Pro Pro Asn Phe Lys Val

2780 2785 2790

Thr Lys Phe Tyr Asp Asp Asn Leu Ile Glu Val Lys Pro Val Ala

2795 2800 2805

Arg Leu Glu Gly Gln Val Asp Leu Tyr Tyr Lys Gly Val Thr Ala

2810 2815 2820

Lys Leu Asp Tyr Asn Asn Gly Lys Val Leu Leu Ala Thr Asn Lys

2825 2830 2835

Trp Glu Val Asp His Ala Phe Leu Thr Arg Leu Val Lys Lys His

2840 2845 2850

Thr Gly Ile Gly Phe Lys Gly Ala Tyr Leu Gly Asp Arg Pro Asp

2855 2860 2865

His Gln Asp Leu Val Asp Arg Asp Cys Ala Thr Ile Thr Lys Asn

2870 2875 2880

Ser Val Gln Phe Leu Lys Met Lys Lys Gly Cys Ala Phe Thr Tyr

2885 2890 2895

Asp Leu Thr Ile Ser Asn Leu Val Arg Leu Ile Glu Leu Val His

2900 2905 2910

Lys Asn Asn Leu Gln Glu Arg Glu Ile Pro Thr Val Thr Val Thr

2915 2920 2925

Thr Trp Leu Ala Tyr Ser Phe Val Asn Glu Asp Leu Gly Thr Ile

2930 2935 2940

Lys Pro Val Leu Gly Glu Lys Val Ile Pro Glu Pro Pro Glu Glu

2945 2950 2955

Leu Ser Leu Gln Pro Thr Val Arg Leu Val Thr Thr Glu Thr Ala

2960 2965 2970

Ile Thr Ile Thr Gly Glu Ala Glu Val Met Thr Thr Gly Ile Thr

2975 2980 2985

Pro Val Val Glu Met Lys Glu Glu Pro Gln Leu Asp His Gln Ser

2990 2995 3000

Thr Thr Leu Lys Val Gly Leu Lys Glu Gly Glu Tyr Pro Gly Pro

3005 3010 3015

Gly Val Asn Pro Asn His Leu Ala Glu Val Ile Asp Glu Lys Asp

3020 3025 3030

Asp Arg Pro Phe Val Leu Ile Ile Gly Asn Lys Gly Ser Thr Ser

3035 3040 3045

Asn Arg Ala Arg Thr Ala Lys Asn Ile Arg Leu Tyr Lys Gly Asn

3050 3055 3060

Asn Pro Arg Glu Ile Arg Asp Leu Met Ser Gln Gly Arg Ile Leu

3065 3070 3075

Thr Val Ala Leu Lys Glu Leu Asp Pro Glu Leu Lys Glu Leu Val

3080 3085 3090

Asp Tyr Lys Gly Thr Phe Leu Asn Arg Glu Ala Leu Glu Ala Leu

3095 3100 3105

Ser Leu Gly Lys Pro Ile Lys Arg Lys Thr Thr Thr Ala Met Ile

3110 3115 3120

Arg Arg Leu Ile Glu Pro Glu Val Glu Glu Glu Leu Pro Asp Trp

3125 3130 3135

Phe Gln Ala Glu Glu Pro Leu Phe Leu Glu Ala Lys Ile Gln Asn

3140 3145 3150

Asp Leu Tyr His Leu Ile Gly Ser Val Asp Ser Ile Lys Ser Lys

3155 3160 3165

Ala Lys Glu Leu Gly Ala Thr Asp Asn Thr Lys Ile Val Lys Glu

3170 3175 3180

Val Gly Ala Arg Thr Tyr Thr Met Lys Leu Ser Ser Trp Ser Thr

3185 3190 3195

Gln Val Thr Lys Lys Gln Met Ser Leu Ala Pro Leu Phe Glu Glu

3200 3205 3210

Leu Leu Leu Lys Cys Pro Pro Cys Ser Lys Ile Ser Lys Gly His

3215 3220 3225

Met Val Ser Ala Tyr Gln Leu Ala Gln Gly Asn Trp Glu Pro Leu

3230 3235 3240

Gly Cys Gly Val Tyr Met Gly Thr Ile Pro Ala Arg Arg Leu Lys

3245 3250 3255

Ile His Pro Tyr Glu Ala Tyr Leu Lys Leu Lys Glu Leu Val Glu

3260 3265 3270

Val Glu Ser Ser Arg Ala Thr Ala Lys Glu Ser Ile Ile Arg Glu

3275 3280 3285

His Asn Thr Trp Ile Leu Arg Lys Val Arg His Glu Gly Asn Leu

3290 3295 3300

Arg Thr Lys Ser Met Ile Asn Pro Gly Lys Ile Ser Asp Gln Leu

3305 3310 3315

Cys Arg Asp Gly His Lys Arg Asn Ile Tyr Asn Lys Ile Ile Gly

3320 3325 3330

Ser Thr Met Ala Ser Ala Gly Ile Arg Leu Glu Lys Leu Pro Val

3335 3340 3345

Val Arg Ala Gln Thr Asp Thr Thr Ser Phe His Gln Ala Ile Arg

3350 3355 3360

Glu Lys Ile Asp Lys Thr Glu Asn Lys Gln Thr Pro Glu Leu His

3365 3370 3375

Glu Glu Leu Met Lys Val Phe Asp Cys Leu Lys Ile Pro Glu Leu

3380 3385 3390

Lys Glu Ser Tyr Asp Glu Val Ser Trp Glu Gln Leu Glu Ala Gly

3395 3400 3405

Ile Asn Arg Lys Gly Ala Ala Gly Tyr Leu Glu Ser Lys Asn Ile

3410 3415 3420

Gly Glu Val Leu Asp Thr Glu Lys His Ile Val Glu Gln Leu Ile

3425 3430 3435

Lys Asp Leu Arg Lys Gly Lys Lys Ile Arg Tyr Tyr Glu Thr Ala

3440 3445 3450

Ile Pro Lys Asn Glu Lys Arg Asp Val Ser Asp Asp Trp Glu Ala

3455 3460 3465

Gly Glu Phe Val Asp Glu Lys Lys Pro Arg Val Ile Gln Tyr Pro

3470 3475 3480

Asp Ala Lys Val Arg Leu Ala Ile Thr Lys Val Met Tyr Lys Trp

3485 3490 3495

Val Lys Gln Lys Pro Val Val Ile Pro Gly Tyr Glu Gly Lys Thr

3500 3505 3510

Pro Leu Phe Asp Ile Phe Asn Lys Val Lys Lys Glu Trp Asp Ser

3515 3520 3525

Phe Gln Asp Pro Val Ala Val Ser Phe Asp Thr Lys Ala Trp Asp

3530 3535 3540

Thr Gln Val Thr Ser Arg Asp Leu Met Leu Ile Lys Asp Ile Gln

3545 3550 3555

Lys Tyr Tyr Phe Lys Arg Ser Ile His Lys Phe Leu Asp Thr Ile

3560 3565 3570

Thr Glu His Met Val Glu Val Pro Val Ile Thr Ala Asp Gly Glu

3575 3580 3585

Val Tyr Ile Arg Asn Gly Gln Arg Gly Ser Gly Gln Pro Asp Thr

3590 3595 3600

Ser Ala Gly Asn Ser Met Leu Asn Val Leu Thr Met Ile Tyr Ala

3605 3610 3615

Phe Cys Lys Ser Thr Gly Ile Pro Tyr Arg Gly Phe Ser Arg Val

3620 3625 3630

Ala Arg Ile His Val Cys Gly Asp Asp Gly Phe Leu Ile Thr Glu

3635 3640 3645

Arg Gly Leu Gly Leu Lys Phe Ser Glu Lys Gly Met Gln Ile Leu

3650 3655 3660

His Glu Ala Gly Lys Pro Gln Lys Ile Thr Glu Gly Asp Lys Met

3665 3670 3675

Lys Val Ala Tyr Arg Phe Glu Asp Ile Glu Phe Cys Ser His Thr

3680 3685 3690

Pro Val Pro Val Arg Trp Ala Asp Asn Thr Ser Ser Tyr Met Ala

3695 3700 3705

Gly Arg Ser Thr Ala Thr Ile Leu Ala Lys Met Ala Thr Arg Leu

3710 3715 3720

Asp Ser Ser Gly Glu Arg Gly Ser Thr Ala Tyr Glu Lys Ala Val

3725 3730 3735

Ala Phe Ser Phe Leu Leu Met Tyr Ser Trp Asn Pro Val Val Arg

3740 3745 3750

Arg Ile Cys Leu Leu Val Leu Ser Gln Phe Pro Glu Ile Ser Pro

3755 3760 3765

Ser Lys Asn Thr Ile Tyr Tyr Tyr Gln Gly Asp Pro Ile Ala Ala

3770 3775 3780

Tyr Arg Glu Val Ile Gly Lys Gln Leu Cys Glu Leu Lys Arg Thr

3785 3790 3795

Gly Phe Glu Lys Leu Ala Gly Leu Asn Leu Ser Met Thr Thr Leu

3800 3805 3810

Gly Ile Trp Thr Lys His Thr Ser Lys Arg Leu Ile Gln Ala Cys

3815 3820 3825

Val Glu Ile Gly Lys Arg Glu Gly Thr Trp Leu Val Asn Ala Asp

3830 3835 3840

Arg Leu Ile Ala Gly Lys Thr Gly Lys Phe Tyr Ile Pro Ser Thr

3845 3850 3855

Gly Val Thr Leu Leu Gly Lys His Tyr Glu Glu Ile Asn Leu Lys

3860 3865 3870

Gln Lys Ala Ala Gln Pro Pro Ile Glu Gly Val Asp Arg Tyr Lys

3875 3880 3885

Leu Gly Pro Ile Val Asn Val Ile Leu Arg Arg Leu Arg Val Met

3890 3895 3900

Leu Met Thr Val Ala Ser Gly Ser Trp

3905 3910

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Met Glu Leu Phe Set Asp Glu Gly Set Lys Gly Ala Thr Ser Lys Lys

1 5 10 15

Gln Pro Lys Pro Asp Arg Ile Glu Lys Gly Lys Met Lys Ile Ala Pro

20 25 30

Lys Glu Thr Glu Lys Asp Cys Lys Thr Arg Pro Pro Asp Ala Thr Ile

35 40 45

Val Val Glu Gly Val Lys Tyr Gln Val Lys Lys Lys Gly Lys Val Arg

50 55 60

Gly Lys Asn Thr Gln Asp Gly Leu Tyr His Asn Lys Asn Lys Pro Pro

65 70 75 80

Glu Set Arg Lys Lys Leu Glu Lys Ala Leu Leu Ala Trp Ala Ile Leu

85 90 95

Ala Ala Val Leu Leu Gln Leu Val Thr Gly Glu Asn Ile Thr Gln Trp

100 105 110

Asn Leu Met Asp Asn Gly Thr Glu Gly Ile Gln Gln Ala Met Phe Leu

115 120 125

Arg Gly Val Asn Arg Ser Leu His Gly Ile Trp Pro Glu Lys Ile Cys

130 135 140

Thr Gly Val Pro Thr His Leu Ala Thr Asp Tyr Glu Leu Lys Glu Ile

145 150 155 160

Val Gly Met Met Asp Ala Ser Glu Lys Thr Asn Tyr Thr Cys Cys Arg

165 170 175

Leu Gln Arg His Glu Trp Asn Lys Gly Trp Cys Asn Trp Phe His Ile

180 185 190

Glu Pro Trp Ile Trp Leu Met Asn Lys Thr Gln Asn Asn Leu Thr Glu

195 200 205

Gly Gln Pro Leu Arg Glu Cys Ala Val Thr Cys Arg Tyr Asp Lys Glu

210 215 220

Thr Glu Leu Asn Ile Val Thr Gln Ala Arg Asp Arg Pro Thr Thr Leu

225 230 235 240

Thr Gly Cys Lys Lys Gly Lys Asn Phe Ser Phe Ala Gly Val Ile Leu

245 250 255

Asp Gly Pro Cys Asn Phe Lys Val Ser Val Glu Asp Val Leu Phe Lys

260 265 270

Glu His Asp Cys Gly Asn Met Leu Gln Glu Thr Ala Ile Gln Leu Leu

275 280 285

Asp Gly Ala Thr Asn Thr Ile Glu Gly Ala Arg Val Gly Thr Ala Lys

290 295 300

Leu Thr Thr Trp Leu Gly Lys Gln Leu Gly Ile Leu Gly Lys Lys Leu

305 310 315 320

Glu Asn Lys Ser Lys Ala Trp Phe Gly Ala His Ala Ala Ser Pro Tyr

325 330 335

Cys Gly Val Glu Arg Lys Ile Gly Tyr Val Trp Tyr Thr Lys Asn Cys

340 345 350

Thr Pro Ala Cys Leu Pro Arg Asn Thr Arg Ile Ile Gly Pro Gly Lys

355 360 365

Phe Asp Thr Asn Ala Glu Asp Gly Lys Ile Leu His Glu Met Gly Gly

370 375 380

His Leu Ser Glu Phe Val Leu Leu Ser Leu Val Val Leu Ser Asp Phe

385 390 395 400

Ala Pro Glu Thr Ala Ser Val Ile Tyr Leu Val Leu His Phe Ala Ile

405 410 415

Pro Gln Ser His Val Asp Val Asp Thr Cys Asp Lys Asn Gln Leu Asn

420 425 430

Leu Thr Val Ala Thr Thr Val Ala Glu Val Ile Pro Gly Thr Val Trp

435 440 445

Asn Leu Gly Lys Tyr Val Cys Ile Arg Pro Asp Trp Trp Pro Tyr Glu

450 455 460

Thr Thr Thr Val Phe Val Ile Glu Glu Ala Gly Gln Val Ile Lys Leu

465 470 475 480

Met Leu Arg Ala Ile Arg Asp Leu Thr Arg Ile Trp Asn Ala Ala Thr

485 490 495

Thr Thr Ala Phe Leu Ile Phe Leu Val Lys Ala Leu Arg Gly Gln Leu

500 505 510

Ile Gln Gly Leu Leu Trp Leu Met Leu Ile Thr Gly Ala Gln Gly Phe

515 520 525

Pro Glu Cys Lys Glu Gly Phe Gln Tyr Ala Ile Ser Lys Asp Arg Lys

530 535 540

Met Gly Leu Leu Gly Pro Glu Ser Leu Thr Thr Thr Trp His Leu Pro

545 550 555 560

Thr Lys Lys Ile Val Asp Ser Met Val His Val Trp Cys Glu Gly Lys

565 570 575

Asp Leu Lys Ile Leu Lys Met Cys Thr Lys Glu Glu Arg Tyr Leu Val

580 585 590

Ala Val His Glu Arg Ala Leu Ser Thr Ser Ala Glu Phe Met Gln Ile

595 600 605

Ser Asp Gly Thr Ile Gly Pro Asp Val Ile Asp Met Pro Asp Asp Phe

610 615 620

Glu Phe Gly Leu Cys Pro Cys Asp Ser Lys Pro Val Ile Lys Gly Lys

625 630 635 640

Phe Asn Ala Ser Leu Leu Asn Gly Pro Ala Phe Gln Met Val Cys Pro

645 650 655

Gln Gly Trp Thr Gly Thr Ile Glu Cys Thr Leu Ala Asn Gln Asp Thr

660 665 670

Leu Asp Thr Thr Val Ile Arg Thr Tyr Arg Arg Thr Thr Pro Phe Gln

675 680 685

Arg Arg Lys Trp Cys Thr Tyr Glu Lys Ile Ile Gly Glu Asp Ile Tyr

690 695 700

Glu Cys Ile Leu Gly Gly Asn Trp Thr Cys Ile Thr Gly Asp His Ser

705 710 715 720

Arg Leu Lys Asp Gly Pro Ile Lys Lys Cys Lys Trp Cys Gly His Asp

725 730 735

Phe Val Asn Ser Glu Gly Leu Pro His Tyr Pro Ile Gly Lys Cys Met

740 745 750

Leu Ile Asn Glu Ser Gly Tyr Arg Tyr Val Asp Asp Thr Ser Cys Asp

755 760 765

Arg Gly Gly Val Ala Ile Val Pro Ser Gly Thr Val Lys Cys Arg Ile

770 775 780

Gly Asn Val Thr Val Gln Val Ile Ala Thr Asn Asn Asp Leu Gly Pro

785 790 795 800

Met Pro Cys Ser Pro Ala Glu Val Ile Ala Ser Glu Gly Pro Val Glu

805 810 815

Lys Thr Ala Cys Thr Phe Asn Tyr Ser Arg Thr Leu Pro Asn Lys Tyr

820 825 830

Tyr Glu Pro Arg Asp Arg Tyr Phe Gln Gln Tyr Met Leu Lys Gly Glu

835 840 845

Trp Gln Tyr Trp Phe Asp Leu Asp Ser Val Asp His His Lys Asp Tyr

850 855 860

Phe Ser Glu Phe Ile Ile Ile Ala Val Val Ala Leu Leu Gly Gly Lys

865 870 875 880

Tyr Val Leu Trp Leu Leu Ile Thr Tyr Thr Ile Leu Ser Glu Gln Met

885 890 895

Ala Met Gly Ala Gly Val Asn Thr Glu Glu Ile Val Met Ile Gly Asn

900 905 910

Leu Leu Thr Asp Ser Asp Ile Glu Val Val Val Tyr Phe Leu Leu Leu

915 920 925

Tyr Leu Ile Val Lys Glu Glu Leu Ala Arg Lys Trp Ile Ile Leu Val

930 935 940

Tyr His Ile Leu Val Ala Asn Pro Met Lys Thr Ile Gly Val Val Leu

945 950 955 960

Leu Met Leu Gly Gly Val Val Lys Ala Ser Arg Ile Asn Ala Asp Asp

965 970 975

Gln Ser Ala Met Asp Pro Cys Phe Leu Leu Val Thr Gly Val Val Ala

980 985 990

Val Leu Met Ile Ala Arg Arg Glu Pro Ala Thr Leu Pro Leu Ile Val

995 1000 1005

Ala Leu Leu Ala Ile Arg Thr Ser Gly Phe Leu Leu Pro Ala Ser

1010 1015 1020

Ile Asp Val Thr Val Ala Val Val Leu Ile Val Leu Leu Leu Ala

1025 1030 1035

Ser Tyr Ile Thr Asp Tyr Phe Arg Tyr Lys Lys Trp Leu Gln Leu

1040 1045 1050

Leu Phe Ser Leu Ile Ala Gly Ile Phe Ile Ile Arg Ser Leu Lys

1055 1060 1065

His Ile Asn Gln Met Glu Val Pro Glu Ile Ser Met Pro Ser Trp

1070 1075 1080

Arg Pro Leu Ala Leu Val Leu Phe Tyr Ile Thr Ser Thr Ala Ile

1085 1090 1095

Thr Thr Asn Trp Asp Ile Asp Leu Ala Gly Phe Leu Leu Gln Trp

1100 1105 1110

Ala Pro Ala Val Ile Met Met Ala Thr Met Trp Ala Asp Phe Leu

1115 1120 1125

Thr Leu Ile Ile Val Leu Pro Ser Tyr Glu Leu Ser Lys Leu Tyr

1130 1135 1140

Phe Leu Lys Asn Val Arg Thr Asp Val Glu Lys Asn Trp Leu Gly

1145 1150 1155

Lys Val Lys Tyr Arg Gln Ile Ser Ser Val Tyr Asp Ile Cys Asp

1160 1165 1170

Ser Glu Glu Ala Val Tyr Leu Phe Pro Ser Arg His Lys Ser Gly

1175 1180 1185

Ser Arg Pro Asp Phe Ile Leu Pro Phe Leu Lys Ala Val Leu Ile

1190 1195 1200

Ser Cys Ile Ser Ser Gln Trp Gln Val Val Tyr Ile Ser Tyr Leu

1205 1210 1215

Ile Leu Glu Ile Thr Tyr Tyr Met His Arg Lys Ile Ile Asp Glu

1220 1225 1230

Val Ser Gly Gly Ala Asn Phe Leu Ser Arg Leu Ile Ala Ala Ile

1235 1240 1245

Ile Glu Leu Asn Trp Ala Ile Asp Asp Glu Glu Cys Lys Gly Leu

1250 1255 1260

Lys Lys Leu Tyr Leu Leu Ser Gly Arg Ala Lys Asn Leu Ile Val

1265 1270 1275

Lys His Lys Val Arg Asn Glu Ala Val His Arg Trp Phe Gly Glu

1280 1285 1290

Glu Glu Ile Tyr Gly Ala Pro Lys Val Ile Thr Ile Ile Lys Ala

1295 1300 1305

Ser Thr Leu Ser Lys Asn Arg His Cys Ile Ile Cys Thr Ile Cys

1310 1315 1320

Glu Gly Lys Glu Trp Asn Gly Ala Asn Cys Pro Lys Cys Gly Arg

1325 1330 1335

Gln Gly Lys Pro Ile Thr Cys Gly Met Thr Leu Ala Asp Phe Glu

1340 1345 1350

Glu Lys His Tyr Lys Lys Ile Phe Ile Arg Glu Glu Ser Ser Cys

1355 1360 1365

Pro Val Pro Phe Asp Pro Ser Cys His Cys Asn Tyr Phe Arg His

1370 1375 1380

Asp Gly Pro Phe Arg Lys Glu Tyr Lys Gly Tyr Val Gln Tyr Thr

1385 1390 1395

Ala Arg Gly Gln Leu Phe Leu Arg Asn Leu Pro Ile Leu Ala Thr

1400 1405 1410

Lys Met Lys Leu Leu Met Val Gly Asn Leu Gly Ala Glu Ile Gly

1415 1420 1425

Asp Leu Glu His Leu Gly Trp Val Leu Arg Gly Pro Ala Val Cys

1430 1435 1440

Lys Lys Ile Thr Asn His Glu Lys Cys His Val Asn Ile Met Asp

1445 1450 1455

Lys Leu Thr Ala Phe Phe Gly Ile Met Pro Arg Gly Thr Thr Pro

1460 1465 1470

Arg Ala Pro Val Arg Phe Pro Thr Ala Leu Leu Lys Val Arg Arg

1475 1480 1485

Gly Leu Glu Thr Gly Trp Ala Tyr Thr His Gln Gly Gly Ile Ser

1490 1495 1500

Ser Val Asp His Val Thr Ala Gly Lys Asp Leu Leu Val Cys Asp

1505 1510 1515

Ser Met Gly Arg Thr Arg Val Val Cys His Ser Asn Asn Lys Met

1520 1525 1530

Thr Asp Glu Thr Glu Tyr Gly Ile Lys Thr Asp Ser Gly Cys Pro

1535 1540 1545

Glu Gly Ala Arg Cys Tyr Val Leu Asn Pro Glu Ala Val Asn Ile

1550 1555 1560

Ser Gly Thr Lys Gly Ala Met Val His Leu Gln Lys Thr Gly Gly

1565 1570 1575

Glu Phe Thr Cys Val Thr Ala Ser Gly Thr Pro Ala Phe Phe Asp

1580 1585 1590

Leu Lys Asn Leu Lys Gly Trp Ser Gly Leu Pro Ile Phe Glu Ala

1595 1600 1605

Ser Ser Gly Arg Val Val Gly Arg Val Lys Val Gly Lys Asn Glu

1610 1615 1620

Asp Ser Lys Pro Thr Lys Leu Met Ser Gly Ile Gln Thr Val Ser

1625 1630 1635

Lys Asn Gln Thr Asp Leu Ala Asp Ile Val Lys Lys Leu Thr Ser

1640 1645 1650

Met Asn Arg Gly Glu Phe Lys Gln Ile Thr Leu Ala Thr Gly Ala

1655 1660 1665

Gly Lys Thr Thr Glu Leu Pro Arg Ser Val Ile Glu Glu Ile Gly

1670 1675 1680

Arg His Lys Arg Val Leu Val Leu Ile Pro Leu Arg Ala Ala Ala

1685 1690 1695

Glu Ser Val Tyr Gln Tyr Met Arg Val Lys Tyr Pro Ser Ile Ser

1700 1705 1710

Phe Asn Leu Arg Ile Gly Asp Met Lys Glu Gly Asp Met Ala Thr

1715 1720 1725

Gly Ile Thr Tyr Ala Ser Tyr Gly Tyr Phe Cys Gln Leu Pro Gln

1730 1735 1740

Pro Lys Leu Arg Ala Ala Met Val Glu Tyr Ser Tyr Ile Phe Leu

1745 1750 1755

Asp Clu Tyr His Cys Ala Thr Pro Glu Gln Leu Ala Ile Ile Gly

1760 1765 1770

Lys Ile His Arg Phe Ala Glu Asn Leu Arg Val Val Ala Met Thr

1775 1780 1785

Ala Thr Pro Ala Gly Thr Val Thr Thr Thr Gly Gln Lys His Pro

1790 1795 1800

Ile Glu Glu Phe Ile Ala Pro Glu Val Met Lys Gly Glu Asp Leu

1805 1810 1815

Gly Ser Glu Tyr Leu Asp Ile Ala Gly Leu Lys Ile Pro Thr Glu

1820 1825 1830

Glu Met Lys Gly Asn Met Leu Val Phe Ala Pro Thr Arg Asn Met

1835 1840 1845

Ala Val Glu Thr Ala Lys Lys Leu Lys Ala Lys Gly Tyr Asn Ser

1850 1855 1860

Gly Tyr Tyr Tyr Ser Gly Glu Asn Pro Glu Asn Leu Arg Val Val

1865 1870 1875

Thr Ser Gln Ser Pro Tyr Val Val Val Ala Thr Asn Ala Ile Glu

1880 1885 1890

Ser Gly Val Thr Leu Pro Asp Leu Asp Thr Val Val Asp Thr Gly

1895 1900 1905

Leu Lys Cys Glu Lys Arg Val Arg Ile Ser Ser Lys Met Pro Phe

1910 1915 1920

Ile Val Thr Gly Leu Lys Arg Met Ala Val Thr Ile Gly Glu Gln

1925 1930 1935

Ala Gln Arg Arg Gly Arg Val Gly Arg Val Lys Pro Gly Arg Tyr

1940 1945 1950

Tyr Arg Ser Gln Glu Thr Ala Ser Gly Ser Lys Asp Tyr His Tyr

1955 1960 1965

Asp Leu Leu Gln Ala Gln Arg Tyr Gly Ile Glu Asp Gly Ile Asn

1970 1975 1980

Val Thr Lys Ser Phe Arg Glu Met Asn Tyr Asp Trp Ser Leu Tyr

1985 1990 1995

Glu Glu Asp Ser Leu Met Ile Thr Gln Leu Glu Val Leu Asn Asn

2000 2005 2010

Leu Leu Ile Ser Glu Asp Leu Pro Ala Ala Val Lys Asn Ile Met

2015 2020 2025

Ala Arg Thr Asp His Pro Glu Pro Ile Gln Leu Ala Tyr Asn Ser

2030 2035 2040

Tyr Glu Asn Gln Ile Pro Val Leu Phe Pro Lys Ile Lys Asn Gly

2045 2050 2055

Glu Val Thr Asp Ser Tyr Glu Asn Tyr Thr Tyr Leu Asn Ala Arg

2060 2065 2070

Lys Leu Gly Glu Asp Val Pro Ala Tyr Val Tyr Ala Thr Glu Asp

2075 2080 2085

Glu Asp Leu Ala Val Asp Leu Leu Gly Met Asp Trp Pro Asp Pro

2090 2095 2100

Gly Asn Gln Gln Val Val Glu Thr Gly Arg Ala Leu Lys Gln Val

2105 2110 2115

Thr Gly Leu Ser Thr Ala Glu Asn Ala Leu Leu Ile Ala Leu Phe

2120 2125 2130

Gly Tyr Val Gly Tyr Gln Thr Leu Ser Lys Arg His Ile Pro Met

2135 2140 2145

Ile Thr Asp Ile Tyr Thr Leu Glu Asp His Arg Leu Glu Asp Thr

2150 2155 2160

Thr His Leu Gln Phe Ala Pro Asn Ala Ile Arg Thr Asp Gly Lys

2165 2170 2175

Asp Ser Glu Leu Lys Glu Leu Ala Val Gly Asp Leu Asp Lys Tyr

2180 2185 2190

Val Asp Ala Leu Val Asp Tyr Ser Lys Gln Gly Met Lys Phe Ile

2195 2200 2205

Lys Val Gln Ala Glu Lys Val Arg Asp Ser Gln Ser Thr Lys Glu

2210 2215 2220

Gly Leu Gln Thr Ile Lys Glu Tyr Val Asp Lys Phe Ile Gln Ser

2225 2230 2235

Leu Thr Glu Asn Lys Glu Glu Ile Ile Arg Tyr Gly Leu Trp Gly

2240 2245 2250

Val His Thr Ala Leu Tyr Lys Ser Leu Ala Ala Arg Leu Gly His

2255 2260 2265

Glu Thr Ala Phe Ala Thr Leu Val Val Lys Trp Leu Ala Phe Gly

2270 2275 2280

Gly Glu Thr Val Ser Ala His Ile Lys Gln Val Ala Val Asp Leu

2285 2290 2295

Val Val Tyr Tyr Ile Ile Asn Lys Pro Ser Phe Pro Gly Asp Thr

2300 2305 2310

Glu Thr Gln Gln Glu Gly Arg Arg Phe Val Ala Ser Leu Phe Ile

2315 2320 2325

Ser Ala Leu Ala Thr Tyr Thr Tyr Lys Thr Trp Asn Tyr Asn Asn

2330 2335 2340

Leu Gln Arg Val Val Glu Pro Ala Leu Ala Tyr Leu Pro Tyr Ala

2345 2350 2355

Thr Ser Ala Leu Lys Leu Phe Thr Pro Thr Arg Leu Glu Ser Val

2360 2365 2370

Val Ile Leu Ser Ser Thr Ile Tyr Lys Thr Tyr Leu Ser Ile Arg

2375 2380 2385

Lys Gly Lys Ser Asp Gly Leu Leu Gly Thr Gly Ile Ser Ala Ala

2390 2395 2400

Met Glu Ile Leu Asn Gln Asn Pro Ile Ser Val Gly Ile Ser Val

2405 2410 2415

Met Leu Gly Val Gly Ala Ile Ala Ala His Asn Ala Ile Glu Ser

2420 2425 2430

Ser Glu Gln Lys Arg Thr Leu Leu Met Lys Val Phe Val Lys Asn

2435 2440 2445

Phe Leu Asp Gln Ala Ala Thr Asp Glu Leu Val Lys Glu Asn Pro

2450 2455 2460

Glu Lys Ile Ile Met Ala Leu Phe Glu Ala Val Gln Thr Ile Gly

2465 2470 2475

Asn Pro Leu Arg Leu Ile Tyr His Leu Tyr Gly Val Tyr Tyr Lys

2480 2485 2490

Gly Trp Glu Ala Lys Glu Leu Ala Glu Lys Thr Ala Gly Arg Asn

2495 2500 2505

Leu Phe Thr Leu Ile Met Phe Glu Ala Phe Glu Leu Leu Gly Met

2510 2515 2520

Asp Ser Glu Gly Lys Ile Arg Asn Leu Ser Gly Asn Tyr Ile Leu

2525 2530 2535

Asp Leu Ile Phe Asn Leu His Asn Lys Leu Asn Lys Gly Leu Lys

2540 2545 2550

Lys Leu Val Leu Gly Trp Ala Pro Ala Pro Leu Ser Cys Asp Trp

2555 2560 2565

Thr Pro Ser Asp Glu Arg Ile Ser Leu Pro His Asn Asn Tyr Leu

2570 2575 2580

Arg Val Glu Thr Arg Cys Pro Cys Gly Tyr Glu Met Lys Ala Ile

2585 2590 2595

Lys Asn Val Ala Gly Lys Leu Thr Lys Val Glu Glu Lys Gly Ser

2600 2605 2610

Phe Leu Cys Arg Asn Arg Leu Gly Arg Gly Pro Pro Asn Phe Lys

2615 2620 2625

Val Thr Lys Phe Tyr Asp Asp Asn Leu Ile Glu Val Lys Pro Val

2630 2635 2640

Ala Arg Leu Glu Gly Gln Val Asp Leu Tyr Tyr Lys Gly Val Thr

2645 2650 2655

Ala Lys Leu Asp Tyr Asn Asn Gly Lys Val Leu Leu Ala Thr Asn

2660 2665 2670

Lys Trp Glu Val Asp His Ala Phe Leu Thr Arg Leu Val Lys Lys

2675 2680 2685

His Thr Gly Ile Gly Phe Lys Gly Ala Tyr Leu Gly Asp Arg Pro

2690 2695 2700

Asp His Gln Asp Leu Val Asp Arg Asp Cys Ala Thr Ile Thr Lys

2705 2710 2715

Asn Ser Val Gln Phe Leu Lys Met Lys Lys Gly Cys Ala Phe Thr

2720 2725 2730

Tyr Asp Leu Thr Ile Ser Asn Leu Val Arg Leu Ile Glu Leu Val

2735 2740 2745

His Lys Asn Asn Leu Gln Glu Arg Glu Ile Pro Thr Val Thr Val

2750 2755 2760

Thr Thr Trp Leu Ala Tyr Ser Phe Val Asn Glu Asp Leu Gly Thr

2765 2770 2775

Ile Lys Pro Val Leu Gly Glu Lys Val Ile Pro Glu Pro Pro Glu

2780 2785 2790

Glu Leu Ser Leu Gln Pro Thr Val Arg Leu Val Thr Thr Glu Thr

2795 2800 2805

Ala Ile Thr Ile Thr Gly Glu Ala Glu Val Met Thr Thr Gly Ile

2810 2815 2820

Thr Pro Val Val Glu Met Lys Glu Glu Pro Gln Leu Asp His Gln

2825 2830 2835

Ser Thr Thr Leu Lys Val Gly Leu Lys Glu Gly Glu Tyr Pro Gly

2840 2845 2850

Pro Gly Val Asn Pro Asn His Leu Ala Glu Val Ile Asp Glu Lys

2855 2860 2865

Asp Asp Arg Pro Phe Val Leu Ile Ile Gly Asn Lys Gly Ser Thr

2870 2875 2880

Ser Asn Arg Ala Arg Thr Ala Lys Asn Ile Arg Leu Tyr Lys Gly

2885 2890 2895

Asn Asn Pro Arg Glu Ile Arg Asp Leu Met Ser Gln Gly Arg Ile

2900 2905 2910

Leu Thr Val Ala Leu Lys Glu Leu Asp Pro Glu Leu Lys Glu Leu

2915 2920 2925

Val Asp Tyr Lys Gly Thr Phe Leu Asn Arg Glu Ala Leu Glu Ala

2930 2935 2940

Leu Ser Leu Gly Lys Pro Ile Lys Arg Lys Thr Thr Thr Ala Met

2945 2950 2955

Ile Arg Arg Leu Ile Glu Pro Glu Val Glu Glu Glu Leu Pro Asp

2960 2965 2970

Trp Phe Gln Ala Glu Glu Pro Leu Phe Leu Glu Ala Lys Ile Gln

2975 2980 2985

Asn Asp Leu Tyr His Leu Ile Gly Ser Val Asp Ser Ile Lys Ser

2990 2995 3000

Lys Ala Lys Glu Leu Gly Ala Thr Asp Asn Thr Lys Ile Val Lys

3005 3010 3015

Glu Val Gly Ala Arg Thr Tyr Thr Met Lys Leu Ser Ser Trp Ser

3020 3025 3030

Thr Gln Val Thr Lys Lys Gln Met Ser Leu Ala Pro Leu Phe Glu

3035 3040 3045

Glu Leu Leu Leu Lys Cys Pro Pro Cys Ser Lys Ile Ser Lys Gly

3050 3055 3060

His Met Val Ser Ala Tyr Gln Leu Ala Gln Gly Asn Trp Glu Pro

3065 3070 3075

Leu Gly Cys Gly Val Tyr Met Gly Thr Ile Pro Ala Arg Arg Leu

3080 3085 3090

Lys Ile His Pro Tyr Glu Ala Tyr Leu Lys Leu Lys Glu Leu Val

3095 3100 3105

Glu Val Glu Ser Ser Arg Ala Thr Ala Lys Glu Ser Ile Ile Arg

3110 3115 3120

Glu His Asn Thr Trp Ile Leu Arg Lys Val Arg His Glu Gly Asn

3125 3130 3135

Leu Arg Thr Lys Ser Met Ile Asn Pro Gly Lys Ile Ser Asp Gln

3140 3145 3150

Leu Cys Arg Asp Gly His Lys Arg Asn Ile Tyr Asn Lys Ile Ile

3155 3160 3165

Gly Ser Thr Met Ala Ser Ala Gly Ile Arg Leu Glu Lys Leu Pro

3170 3175 3180

Val Val Arg Ala Gln Thr Asp Thr Thr Ser Phe His Gln Ala Ile

3185 3190 3195

Arg Glu Lys Ile Asp Lys Thr Glu Asn Lys Gln Thr Pro Glu Leu

3200 3205 3210

His Glu Glu Leu Met Lys Val Phe Asp Cys Leu Lys Ile Pro Glu

3215 3220 3225

Leu Lys Glu Ser Tyr Asp Glu Val Ser Trp Glu Gln Leu Glu Ala

3230 3235 3240

Gly Ile Asn Arg Lys Gly Ala Ala Gly Tyr Leu Glu Ser Lys Asn

3245 3250 3255

Ile Gly Glu Val Leu Asp Thr Glu Lys His Ile Val Glu Gln Leu

3260 3265 3270

Ile Lys Asp Leu Arg Lys Gly Lys Lys Ile Arg Tyr Tyr Glu Thr

3275 3280 3285

Ala Ile Pro Lys Asn Glu Lys Arg Asp Val Ser Asp Asp Trp Glu

3290 3295 3300

Ala Gly Glu Phe Val Asp Glu Lys Lys Pro Arg Val Ile Gln Tyr

3305 3310 3315

Pro Asp Ala Lys Val Arg Leu Ala Ile Thr Lys Val Met Tyr Lys

3320 3325 3330

Trp Val Lys Gln Lys Pro Val Val Ile Pro Gly Tyr Glu Gly Lys

3335 3340 3345

Thr Pro Leu Phe Asp Ile Phe Asn Lys Val Lys Lys Glu Trp Asp

3350 3355 3360

Ser Phe Gln Asp Pro Val Ala Val Ser Phe Asp Thr Lys Ala Trp

3365 3370 3375

Asp Thr Gln Val Thr Ser Arg Asp Leu Met Leu Ile Lys Asp Ile

3380 3385 3390

Gln Lys Tyr Tyr Phe Lys Arg Ser Ile His Lys Phe Leu Asp Thr

3395 3400 3405

Ile Thr Glu His Met Val Glu Val Pro Val Ile Thr Ala Asp Gly

3410 3415 3420

Glu Val Tyr Ile Arg Asn Gly Gln Arg Gly Ser Gly Gln Pro Asp

3425 3430 3435

Thr Ser Ala Gly Asn Ser Met Leu Asn Val Leu Thr Met Ile Tyr

3440 3445 3450

Ala Phe Cys Lys Ser Thr Gly Ile Pro Tyr Arg Gly Phe Ser Arg

3455 3460 3465

Val Ala Arg Ile His Val Cys Gly Asp Asp Gly Phe Leu Ile Thr

3470 3475 3480

Glu Arg Gly Leu Gly Leu Lys Phe Ser Glu Lys Gly Met Gln Ile

3485 3490 3495

Leu His Glu Ala Gly Lys Pro Gln Lys Ile Thr Glu Gly Asp Lys

3500 3505 3510

Met Lys Val Ala Tyr Arg Phe Glu Asp Ile Glu Phe Cys Ser His

3515 3520 3525

Thr Pro Val Pro Val Arg Trp Ala Asp Asn Thr Ser Ser Tyr Met

3530 3535 3540

Ala Gly Arg Ser Thr Ala Thr Ile Leu Ala Lys Met Ala Thr Arg

3545 3550 3555

Leu Asp Ser Ser Gly Glu Arg Gly Ser Thr Ala Tyr Glu Lys Ala

3560 3565 3570

Val Ala Phe Ser Phe Leu Leu Met Tyr Ser Trp Asn Pro Val Val

3575 3580 3585

Arg Arg Ile Cys Leu Leu Val Leu Ser Gln Phe Pro Glu Ile Ser

3590 3595 3600

Pro Ser Lys Asn Thr Ile Tyr Tyr Tyr Gln Gly Asp Pro Ile Ala

3605 3610 3615

Ala Tyr Arg Glu Val Ile Gly Lys Gln Leu Cys Glu Leu Lys Arg

3620 3625 3630

Thr Gly Phe Glu Lys Leu Ala Cly Leu Asn Leu Ser Met Thr Thr

3635 3640 3645

Leu Gly Ile Trp Thr Lys His Thr Ser Lys Arg Leu Ile Gln Ala

3650 3655 3660

Cys Val Glu Ile Gly Lys Arg Glu Gly Thr Trp Leu Val Asn Ala

3665 3670 3675

Asp Arg Leu Ile Ala Gly Lys Thr Gly Lys Phe Tyr Ile Pro Ser

3680 3685 3690

Thr Gly Val Thr Leu Leu Gly Lys His Tyr Glu Glu Ile Asn Leu

3695 3700 3705

Lys Gln Lys Ala Ala Gln Pro Pro Ile Glu Gly Val Asp Arg Tyr

3710 3715 3720

Lys Leu Gly Pro Ile Val Asn Val Ile Leu Arg Arg Leu Arg Val

3725 3730 3735

Met Leu Met Thr Val Ala Ser Gly Ser Trp

3740 3745

<210>9

<211>3913

<212>PRT

<213>人工序列：XIKE-C BVDV-序列

<400>9

Met Glu Leu Phe Ser Asn Glu Leu Leu Tyr Lys Thr Tyr Lys Gln Lys

1 5 10 15

Pro Ala Gly Val Val Glu Pro Val Tyr Asp Val Asn Gly Arg Pro Leu

20 25 30

Phe Gly Glu Ser Ser Asp Leu His Pro Gln Ser Thr Leu Lys Leu Pro

35 40 45

His Gln Arg Gly Ser Ala Asn Ile Leu Thr Asn Ala Arg Ser Leu Pro

50 55 60

Arg Lys Gly Asp Cys Arg Arg Gly Asn Val Tyr Gly Pro Val Ser Gly

65 70 75 80

Ile Tyr Ile Lys Pro Gly Pro Ile Tyr Tyr Gln Asp Tyr Val Gly Pro

85 90 95

Val Tyr His Arg Ala Pro Leu Glu Leu Cys Arg Glu Ala Ser Met Cys

100 105 110

Glu Thr Thr Arg Arg Val Gly Arg Val Thr Gly Ser Asp Gly Lys Leu

115 120 125

Tyr His Ile Tyr Ile Cys Ile Asp Gly Cys Ile Leu Leu Lys Arg Ala

130 135 140

Thr Arg Asn Gln Pro Glu Val Leu Lys Trp Val Tyr Asn Arg Leu Asn

145 150 155 160

Cys Pro Leu Trp Val Thr Ser Cys Ser Asp Glu Gly Ser Lys Gly Ala

165 170 175

Thr Ser Lys Lys Gln Pro Lys Pro Asp Arg Ile Glu Lys Gly Lys Met

180 185 190

Lys Ile Ala Pro Lys Glu Thr Glu Lys Asp Cys Lys Thr Arg Pro Pro

195 200 205

Asp Ala Thr Ile Val Val Glu Gly Val Lys Tyr Gln Val Lys Lys Lys

210 215 220

Gly Lys Val Arg Gly Lys Asn Thr Gln Asp Gly Leu Tyr His Asn Lys

225 230 235 240

Asn Lys Pro Pro Glu Ser Arg Lys Lys Leu Glu Lys Ala Leu Leu Ala

245 250 255

Trp Ala Ile Leu Ala Ala Val Leu Leu Gln Leu Val Thr Gly Glu Asn

260 265 270

Ile Thr Gln Trp Asn Leu Met Asp Asn Gly Thr Glu Gly Ile Gln Gln

275 280 285

Ala Met Phe Leu Arg Gly Val Asn Arg Ser Leu Leu Gly Ile Trp Pro

290 295 300

Glu Lys Ile Cys Thr Gly Val Pro Thr His Leu Ala Thr Asp Tyr Glu

305 310 315 320

Leu Lys Glu Ile Val Gly Met Met Asp Ala Ser Glu Lys Thr Asn Tyr

325 330 335

Thr Cys Cys Arg Leu Gln Arg His Glu Trp Asn Lys His Gly Trp Cys

340 345 350

Asn Trp Phe His Ile Glu Pro Trp Ile Trp Leu Met Asn Lys Thr Gln

355 360 365

Asn Asn Leu Thr Glu Gly Gln Pro Leu Arg Glu Cys Ala Val Thr Cys

370 375 380

Arg Tyr Asp Lys Glu Thr Glu Leu Asn Ile Val Thr Gln Ala Arg Asp

385 390 395 400

Arg Pro Thr Thr Leu Thr Gly Cys Lys Lys Gly Lys Asn Phe Ser Phe

405 410 415

Ala Gly Val Ile Leu Asp Gly Pro Cys Asn Phe Lys Val Ser Val Glu

420 425 430

Asp Val Leu Phe Lys Glu His Asp Cys Gly Asn Met Leu Gln Glu Thr

435 440 445

Ala Ile Gln Leu Leu Asp Gly Ala Thr Asn Thr Ile Glu Gly Ala Arg

450 455 460

Val Gly Thr Ala Lys Leu Thr Thr Trp Leu Gly Lys Gln Leu Gly Ile

465 470 475 480

Leu Gly Lys Lys Leu Glu Asn Lys Ser Lys Ala Trp Phe Gly Ala His

485 490 495

Ala Ala Ser Pro Tyr Cys Gly Val Glu Arg Lys Ile Gly Tyr Val Trp

500 505 510

Tyr Thr Lys Asn Cys Thr Pro Ala Cys Leu Pro Arg Asn Thr Arg Ile

515 520 525

Ile Gly Pro Gly Lys Phe Asp Thr Asn Ala Glu Asp Gly Lys Ile Leu

530 535 540

His Glu Met Gly Gly His Leu Ser Glu Phe Val Leu Leu Ser Leu Val

545 550 555 560

Val Leu Ser Asp Phe Ala Pro Glu Thr Ala Ser Val Ile Tyr Leu Val

565 570 575

Leu His Phe Ala Ile Pro Gln Ser His Val Asp Val Asp Thr Cys Asp

580 585 590

Lys Asn Gln Leu Asn Leu Thr Val Ala Thr Thr Val Ala Glu Val Ile

595 600 605

Pro Gly Thr Val Trp Asn Leu Gly Lys Tyr Val Cys Ile Arg Pro Asp

610 615 620

Trp Trp Pro Tyr Glu Thr Thr Thr Val Phe Val Ile Glu Glu Ala Gly

625 630 635 640

Gln Val Ile Lys Leu Met Leu Arg Ala Ile Arg Asp Leu Thr Arg Ile

645 650 655

Trp Asn Ala Ala Thr Thr Thr Ala Phe Leu Ile Phe Leu Val Lys Ala

660 665 670

Leu Arg Gly Gln Leu Ile Gln Gly Leu Leu Trp Leu Met Leu Ile Thr

675 680 685

Gly Ala Gln Gly Phe Pro Glu Cys Lys Glu Gly Phe Gln Tyr Ala Ile

690 695 700

Ser Lys Asp Arg Lys Met Gly Leu Leu Gly Pro Glu Ser Leu Thr Thr

705 710 715 720

Thr Trp His Leu Pro Thr Lys Lys Ile Val Asp Ser Met Val His Val

725 730 735

Trp Cys Glu Gly Lys Asp Leu Lys Ile Leu Lys Met Cys Thr Lys Glu

740 745 750

Glu Arg Tyr Leu Val Ala Val His Glu Arg Ala Leu Ser Thr Ser Ala

755 760 765

Glu Phe Met Gln Ile Ser Asp Gly Thr Ile Gly Pro Asp Val Ile Asp

770 775 780

Met Pro Asp Asp Phe Glu Phe Gly Leu Cys Pro Cys Asp Ser Lys Pro

785 790 795 800

Val Ile Lys Gly Lys Phe Asn Ala Ser Leu Leu Asn Gly Pro Ala Phe

805 810 815

Gln Met Val Cys Pro Gln Gly Trp Thr Gly Thr Ile Glu Cys Thr Leu

820 825 830

Ala Asn Gln Asp Thr Leu Asp Thr Thr Val Ile Arg Thr Tyr Arg Arg

835 840 845

Thr Thr Pro Phe Gln Arg Arg Lys Trp Cys Thr Tyr Glu Lys Ile Ile

850 855 860

Gly Glu Asp Ile Tyr Glu Cys Tle Leu Gly Gly Asn Trp Thr Cys Ile

865 870 875 880

Thr Gly Asp His Ser Arg Leu Lys Asp Gly Pro Ile Lys Lys Cys Lys

885 890 895

Trp Cys Gly His Asp Phe Val Asn Ser Glu Gly Leu Pro His Tyr Pro

900 905 910

Ile Gly Lys Cys Met Leu Ile Asn Glu Ser Gly Tyr Arg Tyr Val Asp

915 920 925

Asp Thr Ser Cys Asp Arg Gly Gly Val Ala Ile Val Pro Ser Gly Thr

930 935 940

Val Lys Cys Arg Ile Gly Asn Val Thr Val Gln Val Ile Ala Thr Asn

945 950 955 960

Asn Asp Leu Gly Pro Met Pro Cys Ser Pro Ala Glu Val Ile Ala Ser

965 970 975

Glu Gly Pro Val Glu Lys Thr Ala Cys Thr Phe Asn Tyr Ser Arg Thr

980 985 990

Ieu Pro Asn Lys Tyr Tyr Glu Pro Arg Asp Arg Tyr Phe Gln Gln Tyr

995 1000 1005

Met Leu Lys Gly Glu Trp Gln Tyr Trp Phe Asp Leu Asp Ser Val

1010 1015 1020

Asp His His Lys Asp Tyr Phe Ser Glu Phe Ile Ile Ile Ala Val

1025 1030 1035

Val Ala Leu Leu Gly Gly Lys Tyr Val Leu Trp Leu Leu Ile Thr

1040 1045 1050

Tyr Thr Ile Leu Ser Glu Gln Met Ala Met Gly Ala Gly Val Asn

1055 1060 1065

Thr Glu Glu Ile Val Met Ile Gly Asn Leu Leu Thr Asp Ser Asp

1070 1075 1080

Ile Glu Val Val Val Tyr Phe Leu Leu Leu Tyr Leu Ile Val Lys

1085 1090 1095

Glu Glu Leu Ala Arg Lys Trp Ile Ile Leu Val Tyr His Ile Leu

1100 1105 1110

Val Ala Asn Pro Met Lys Thr Ile Gly Val Val Leu Leu Met Leu

1115 1120 1125

Gly Gly Val Val Lys Ala Ser Arg Ile Asn Ala Asp Asp Gln Ser

1130 1135 1140

Ala Met Asp Pro Cys Phe Leu Leu Val Thr Gly Val Val Ala Val

1145 1150 1155

Leu Met Ile Ala Arg Arg Glu Pro Ala Thr Leu Pro Leu Ile Val

1160 1165 1170

Ala Leu Leu Ala Ile Arg Thr Ser Gly Phe Leu Leu Pro Ala Ser

1175 1180 1185

Ile Asp Val Thr Val Ala Val Val Leu Ile Val Leu Leu Leu Ala

1190 1195 1200

Ser Tyr Ile Thr Asp Tyr Phe Arg Tyr Lys Lys Trp Leu Gln Leu

1205 1210 1215

Leu Phe Ser Leu Ile Ala Gly Ile Phe Ile Ile Arg Ser Leu Lys

1220 1225 1230

His Ile Asn Gln Met Glu Val Pro Glu Ile Ser Met Pro Ser Trp

1235 1240 1245

Arg Pro Leu Ala Leu Val Leu Phe Tyr Ile Thr Ser Thr Ala Ile

1250 1255 1260

Thr Thr Asn Trp Asp Ile Asp Leu Ala Gly Phe Leu Leu Gln Trp

1265 1270 1275

Ala Pro Ala Val Ile Met Met Ala Thr Met Trp Ala Asp Phe Leu

1280 1285 1290

Thr Leu Ile Ile Val Leu Pro Ser Tyr Glu Leu Ser Lys Leu Tyr

1295 1300 1305

Phe Leu Lys Asn Val Arg Thr Asp Val Glu Lys Asn Trp Leu Gly

1310 1315 1320

Lys Val Lys Tyr Arg Gln Ile Ser Ser Val Tyr Asp Ile Cys Asp

1325 1330 1335

Ser Glu Glu Ala Val Tyr Leu Phe Pro Ser Arg His Lys Ser Gly

1340 1345 1350

Ser Arg Pro Asp Phe Ile Leu Pro Phe Leu Lys Ala Val Leu Ile

1355 1360 1365

Ser Cys Ile Ser Ser Gln Trp Gln Val Val Tyr Ile Ser Tyr Leu

1370 1375 1380

Ile Leu Glu Ile Thr Tyr Tyr Met His Arg Lys Ile Ile Asp Glu

1385 1390 1395

Val Ser Gly Gly Ala Asn Phe Leu Ser Arg Leu Ile Ala Ala Ile

1400 1405 1410

Ile Glu Leu Asn Trp Ala Ile Asp Asp Glu Glu Cys Lys Gly Leu

1415 1420 1425

Lys Lys Leu Tyr Leu Leu Ser Gly Arg Ala Lys Asn Leu Ile Val

1430 1435 1440

Lys His Lys Val Arg Asn Glu Ala Val His Arg Trp Phe Gly Glu

1445 1450 1455

Glu Glu Ile Tyr Gly Ala Pro Lys Val Ile Thr Ile Ile Lys Ala

1460 1465 1470

Ser Thr Leu Ser Lys Asn Arg His Cys Ile Ile Cys Thr Ile Cys

1475 1480 1485

Glu Gly Lys Glu Trp Asn Gly Ala Asn Cys Pro Lys Cys Gly Arg

1490 1495 1500

Gln Gly Lys Pro Ile Thr Cys Gly Met Thr Leu Ala Asp Phe Glu

1505 1510 1515

Glu Lys His Tyr Lys Lys Ile Phe Ile Arg Glu Glu Ser Ser Cys

1520 1525 1530

Pro Val Pro Phe Asp Pro Ser Cys His Cys Asn Tyr Phe Arg His

1535 1540 1545

Asp Gly Pro Phe Arg Lys Glu Tyr Lys Gly Tyr Val Gln Tyr Thr

1550 1555 1560

Ala Arg Gly Gln Leu Phe Leu Arg Asn Leu Pro Ile Leu Ala Thr

1565 1570 1575

Lys Met Lys Leu Leu Met Val Gly Asn Leu Gly Ala Glu Ile Gly

1580 1585 1590

Asp Leu Glu His Leu Gly Trp Val Leu Arg Gly Pro Ala Val Cys

1595 1600 1605

Lys Lys Ile Thr Asn His Glu Lys Cys His Val Asn Ile Met Asp

1610 1615 1620

Lys Leu Thr Ala Phe Phe Gly Ile Met Pro Arg Gly Thr Thr Pro

1625 1630 1635

Arg Ala Pro Val Arg Phe Pro Thr Ala Leu Leu Lys Val Arg Arg

1640 1645 1650

Gly Leu Glu Thr Gly Trp Ala Tyr Thr His Gln Gly Gly Ile Ser

1655 1660 1665

Ser Val Asp His Val Thr Ala Gly Lys Asp Leu Leu Val Cys Asp

1670 1675 1680

Ser Met Gly Arg Thr Arg Val Val Cys His Ser Asn Asn Lys Met

1685 1690 1695

Thr Asp Glu Thr Glu Tyr Gly Ile Lys Thr Asp Ser Gly Cys Pro

1700 1705 1710

Glu Gly Ala Arg Cys Tyr Val Leu Asn Pro Glu Ala Val Asn Ile

1715 1720 1725

Ser Gly Thr Lys Gly Ala Met Val His Leu Gln Lys Thr Gly Gly

1730 1735 1740

Glu Phe Thr Cys Val Thr Ala Ser Gly Thr Pro Ala Phe Phe Asp

1745 1750 1755

Leu Lys Asn Leu Lys Gly Trp Ser Gly Leu Pro Ile Phe Glu Ala

1760 1765 1770

Ser Ser Gly Arg Val Val Gly Arg Val Lys Val Gly Lys Asn Glu

1775 1780 1785

Asp Ser Lys Pro Thr Lys Leu Met Ser Gly Ile Gln Thr Val Ser

1790 1795 1800

Lys Asn Gln Thr Asp Leu Ala Asp Ile Val Lys Lys Leu Thr Ser

1805 1810 1815

Met Asn Arg Gly Glu Phe Lys Gln Ile Thr Leu Ala Thr Gly Ala

1820 1825 1830

Gly Lys Thr Thr Glu Leu Pro Arg Ser Val Ile Glu Glu Ile Gly

1835 1840 1845

Arg His Lys Arg Val Leu Val Leu Ile Pro Leu Arg Ala Ala Ala

1850 1855 1860

Glu Ser Val Tyr Gln Tyr Met Arg Val Lys Tyr Pro Ser Ile Ser

1865 1870 1875

Phe Asn Leu Arg Ile Gly Asp Met Lys Glu Gly Asp Met Ala Thr

1880 1885 1890

Gly Ile Thr Tyr Ala Ser Tyr Gly Tyr Phe Cys Gln Leu Pro Gln

1895 1900 1905

Pro Lys Leu Arg Ala Ala Met Val Glu Tyr Ser Tyr Ile Phe Leu

1910 1915 1920

Asp Glu Tyr His Cys Ala Thr Pro Glu Gln Leu Ala Ile Ile Gly

1925 1930 1935

Lys Ile His Arg Phe Ala Glu Asn Leu Arg Val Val Ala Met Thr

1940 1945 1950

Ala Thr Pro Ala Gly Thr Val Thr Thr Thr Gly Gln Lys His Pro

1955 1960 1965

Ile Glu Glu Phe Ile Ala Pro Glu Val Met Lys Gly Glu Asp Leu

1970 1975 1980

Gly Ser Glu Tyr Leu Asp Ile Ala Gly Leu Lys Ile Pro Thr Glu

1985 1990 1995

Glu Met Lys Gly Asn Met Leu Val Phe Ala Pro Thr Arg Asn Met

2000 2005 2010

Ala Val Glu Thr Ala Lys Lys Leu Lys Ala Lys Gly Tyr Asn Ser

2015 2020 2025

Gly Tyr Tyr Tyr Ser Gly Glu Asn Pro Glu Asn Leu Arg Val Val

2030 2035 2040

Thr Ser Gln Ser Pro Tyr Val Val Val Ala Thr Asn Ala Ile Glu

2045 2050 2055

Ser Gly Val Thr Leu Pro Asp Leu Asp Thr Val Val Asp Thr Gly

2060 2065 2070

Leu Lys Cys Glu Lys Arg Val Arg Ile Ser Ser Lys Met Pro Phe

2075 2080 2085

Ile Val Thr Gly Leu Lys Arg Met Ala Val Thr Ile Gly Glu Gln

2090 2095 2100

Ala Gln Arg Arg Gly Arg Val Gly Arg Val Lys Pro Gly Arg Tyr

2105 2110 2115

Tyr Arg Ser Gln Glu Thr Ala Ser Gly Ser Lys Asp Tyr His Tyr

2120 2125 2130

Asp Leu Leu Gln Ala Gln Arg Tyr Gly Ile Glu Asp Gly Ile Asn

2135 2140 2145

Val Thr Lys Ser Phe Arg Glu Met Asn Tyr Asp Trp Ser Leu Tyr

2150 2155 2160

Glu Glu Asp Ser Leu Met Ile Thr Gln Leu Glu Val Leu Asn Asn

2165 2170 2175

Leu Leu Ile Ser Glu Asp Leu Pro Ala Ala Val Lys Asn Ile Met

2180 2185 2190

Ala Arg Thr Asp His Pro Glu Pro Ile Gln Leu Ala Tyr Asn Ser

2195 2200 2205

Tyr Glu Asn Gln Ile Pro Val Leu Phe Pro Lys Ile Lys Asn Gly

2210 2215 2220

Glu Val Thr Asp Ser Tyr Glu Asn Tyr Thr Tyr Leu Asn Ala Arg

2225 2230 2235

Lys Leu Gly Glu Asp Val Pro Ala Tyr Val Tyr Ala Thr Glu Asp

2240 2245 2250

Glu Asp Leu Ala Val Asp Leu Leu Gly Met Asp Trp Pro Asp Pro

2255 2260 2265

Gly Asn Gln Gln Val Val Glu Thr Gly Arg Ala Leu Lys Gln Val

2270 2275 2280

Thr Gly Leu Ser Thr Ala Glu Asn Ala Leu Leu Ile Ala Leu Phe

2285 2290 2295

Gly Tyr Val Gly Tyr Gln Thr Leu Ser Lys Arg His Ile Pro Met

2300 2305 2310

Ile Thr Asp Ile Tyr Thr Leu Glu Asp His Arg Leu Glu Asp Thr

2315 2320 2325

Thr His Leu Gln Phe Ala Pro Asn Ala Ile Arg Thr Asp Gly Lys

2330 2335 2340

Asp Ser Glu Leu Lys Glu Leu Ala Val Gly Asp Leu Asp Lys Tyr

2345 2350 2355

Val Asp Ala Leu Val Asp Tyr Ser Lys Gln Gly Met Lys Phe Ile

2360 2365 2370

Lys Val Gln Ala Glu Lys Val Arg Asp Ser Gln Ser Thr Lys Glu

2375 2380 2385

Gly Leu Gln Thr Ile Lys Glu Tyr Val Asp Lys Phe Ile Gln Ser

2390 2395 2400

Leu Thr Glu Asn Lys Glu Glu Ile Ile Arg Tyr Gly Leu Trp Gly

2405 2410 2415

Val His Thr Ala Leu Tyr Lys Ser Leu Ala Ala Arg Leu Gly His

2420 2425 2430

Glu Thr Ala Phe Ala Thr Leu Val Val Lys Trp Leu Ala Phe Gly

2435 2440 2445

Gly Glu Thr Val Ser Ala His Ile Lys Gln Val Ala Val Asp Leu

2450 2455 2460

Val Val Tyr Tyr Ile Ile Asn Lys Pro Ser Phe Pro Gly Asp Thr

2465 2470 2475

Glu Thr Gln Gln Glu Gly Arg Arg Phe Val Ala Ser Leu Phe Ile

2480 2485 2490

Ser Ala Leu Ala Thr Tyr Thr Tyr Lys Thr Trp Asn Tyr Asn Asn

2495 2500 2505

Leu Gln Arg Val Val Glu Pro Ala Leu Ala Tyr Leu Pro Tyr Ala

2510 2515 2520

Thr Ser Ala Leu Lys Leu Phe Thr Pro Thr Arg Leu Glu Ser Val

2525 2530 2535

Val Ile Leu Ser Ser Thr Ile Tyr Lys Thr Tyr Leu Ser Ile Arg

2540 2545 2550

Lys Gly Lys Ser Asp Gly Leu Leu Gly Thr Gly Ile Ser Ala Ala

2555 2560 2565

Met Glu Ile Leu Asn Gln Asn Pro Ile Ser Val Gly Ile Ser Val

2570 2575 2580

Met Leu Gly Val Gly Ala Ile Ala Ala His Asn Ala Ile Glu Ser

2585 2590 2595

Ser Glu Gln Lys Arg Thr Leu Leu Met Lys Val Phe Val Lys Asn

2600 2605 2610

Phe Leu Asp Gln Ala Ala Thr Asp Glu Leu Val Lys Glu Asn Pro

2615 2620 2625

Glu Lys Ile Ile Met Ala Leu Phe Glu Ala Val Gln Thr Ile Gly

2630 2635 2640

Asn Pro Leu Arg Leu Ile Tyr His Leu Tyr Gly Val Tyr Tyr Lys

2645 2650 2655

Gly Trp Glu Ala Lys Glu Leu Ala Glu Lys Thr Ala Gly Arg Asn

2660 2665 2670

Leu Phe Thr Leu Ile Met Phe Glu Ala Phe Glu Leu Leu Gly Met

2675 2680 2685

Asp Ser Glu Gly Lys Ile Arg Asn Leu Ser Gly Asn Tyr Ile Leu

2690 2695 2700

Asp Leu Ile Phe Asn Leu His Asn Lys Leu Asn Lys Gly Leu Lys

2705 2710 2715

Lys Leu Val Leu Gly Trp Ala Pro Ala Pro Leu Ser Cys Asp Trp

2720 2725 2730

Thr Pro Ser Asp Glu Arg Ile Ser Leu Pro His Asn Asn Tyr Leu

2735 2740 2745

Arg Val Glu Thr Arg Cys Pro Cys Gly Tyr Glu Met Lys Ala Ile

2750 2755 2760

Lys Asn Val Ala Gly Lys Leu Thr Lys Val Glu Glu Lys Gly Ser

2765 2770 2775

Phe Leu Cys Arg Asn Arg Leu Gly Arg Gly Pro Pro Asn Phe Lys

2780 2785 2790

Val Thr Lys Phe Tyr Asp Asp Asn Leu Ile Glu Val Lys Pro Val

2795 2800 2805

Ala Arg Leu Glu Gly Gln Val Asp Leu Tyr Tyr Lys Gly Val Thr

2810 2815 2820

Ala Lys Leu Asp Tyr Asn Asn Gly Lys Val Leu Leu Ala Thr Asn

2825 2830 2835

Lys Trp Glu Val Asp His Ala Phe Leu Thr Arg Leu Val Lys Lys

2840 2845 2850

His Thr Gly Ile Gly Phe Lys Gly Ala Tyr Leu Gly Asp Arg Pro

2855 2860 2865

Asp His Gln Asp Leu Val Asp Arg Asp Cys Ala Thr Ile Thr Lys

2870 2875 2880

Asn Ser Val Gln Phe Leu Lys Met Lys Lys Gly Cys Ala Phe Thr

2885 2890 2895

Tyr Asp Leu Thr Ile Ser Asn Leu Val Arg Leu Ile Glu Leu Val

2900 2905 2910

His Lys Asn Asn Leu Gln Glu Arg Glu Ile Pro Thr Val Thr Val

2915 2920 2925

Thr Thr Trp Leu Ala Tyr Ser Phe Val Asn Glu Asp Leu Gly Thr

2930 2935 2940

Ile Lys Pro Val Leu Gly Glu Lys Val Ile Pro Glu Pro Pro Glu

2945 2950 2955

Glu Leu Ser Leu Gln Pro Thr Val Arg Leu Val Thr Thr Glu Thr

2960 2965 2970

Ala Ile Thr Ile Thr Gly Glu Ala Glu Val Met Thr Thr Gly Ile

2975 2980 2985

Thr Pro Val Val Glu Met Lys Glu Glu Pro Gln Leu Asp His Gln

2990 2995 3000

Ser Thr Thr Leu Lys Val Gly Leu Lys Glu Gly Glu Tyr Pro Gly

3005 3010 3015

Pro Gly Val Asn Pro Asn His Leu Ala Glu Val Ile Asp Glu Lys

3020 3025 3030

Asp Asp Arg Pro Phe Val Leu Ile Ile Gly Asn Lys Gly Ser Thr

3035 3040 3045

Ser Asn Arg Ala Arg Thr Ala Lys Asn Ile Arg Leu Tyr Lys Gly

3050 3055 3060

Asn Asn Pro Arg Glu Ile Arg Asp Leu Met Ser Gln Gly Arg Ile

3065 3070 3075

Leu Thr Val Ala Leu Lys Glu Leu Asp Pro Glu Leu Lys Glu Leu

3080 3085 3090

Val Asp Tyr Lys Gly Thr Phe Leu Asn Arg Glu Ala Leu Glu Ala

3095 3100 3105

Leu Ser Leu Gly Lys Pro Ile Lys Arg Lys Thr Thr Thr Ala Met

3110 3115 3120

Ile Arg Arg Leu Ile Glu Pro Glu Val Glu Glu Glu Leu Pro Asp

3125 3130 3135

Trp Phe Gln Ala Glu Glu Pro Leu Phe Leu Glu Ala Lys Ile Gln

3140 3145 3150

Asn Asp Leu Tyr His Leu Ile Gly Ser Val Asp Ser Ile Lys Ser

3155 3160 3165

Lys Ala Lys Glu Leu Gly Ala Thr Asp Asn Thr Lys Ile Val Lys

3170 3175 3180

Glu Val Gly Ala Arg Thr Tyr Thr Met Lys Leu Ser Ser Trp Ser

3185 3190 3195

Thr Gln Val Thr Lys Lys Gln Met Ser Leu Ala Pro Leu Phe Glu

3200 3205 3210

Glu Leu Leu Leu Lys Cys Pro Pro Cys Ser Lys Ile Ser Lys Gly

3215 3220 3225

His Met Val Ser Ala Tyr Gln Leu Ala Gln Gly Asn Trp Glu Pro

3230 3235 3240

Ieu Gly Cys Gly Val Tyr Met Gly Thr Ile Pro Ala Arg Arg Leu

3245 3250 3255

Lys Ile His Pro Tyr Glu Ala Tyr Leu Lys Leu Lys Glu Leu Val

3260 3265 3270

Glu Val Glu Ser Ser Arg Ala Thr Ala Lys Glu Ser Ile Ile Arg

3275 3280 3285

Glu His Asn Thr Trp Ile Leu Arg Lys Val Arg His Glu Gly Asn

3290 3295 3300

Leu Arg Thr Lys Ser Met Ile Asn Pro Gly Lys Ile Ser Asp Gln

3305 3310 3315

Leu Cys Arg Asp Gly His Lys Arg Asn Ile Tyr Asn Lys Ile Ile

3320 3325 3330

Gly Ser Thr Met Ala Ser Ala Gly Ile Arg Leu Glu Lys Leu Pro

3335 3340 3345

Val Val Arg Ala Gln Thr Asp Thr Thr Ser Phe His Gln Ala Ile

3350 3355 3360

Arg Glu Lys Ile Asp Lys Thr Glu Asn Lys Gln Thr Pro Glu Leu

3365 3370 3375

His Glu Glu Leu Met Lys Val Phe Asp Cys Leu Lys Ile Pro Glu

3380 3385 3390

Leu Lys Glu Ser Tyr Asp Glu Val Ser Trp Glu Gln Leu Glu Ala

3395 3400 3405

Gly Ile Asn Arg Lys Gly Ala Ala Gly Tyr Leu Glu Ser Lys Asn

3410 3415 3420

Ile Gly Glu Val Leu Asp Thr Glu Lys His Ile Val Glu Gln Leu

3425 3430 3435

Ile Lys Asp Leu Arg Lys Gly Lys Lys Ile Arg Tyr Tyr Glu Thr

3440 3445 3450

Ala Ile Pro Lys Asn Glu Lys Arg Asp Val Ser Asp Asp Trp Glu

3455 3460 3465

Ala Gly Glu Phe Val Asp Glu Lys Lys Pro Arg Val Ile Gln Tyr

3470 3475 3480

Pro Asp Ala Lys Val Arg Leu Ala Ile Thr Lys Val Met Tyr Lys

3485 3490 3495

Trp Val Lys Gln Lys Pro Val Val Ile Pro Gly Tyr Glu Gly Lys

3500 3505 3510

Thr Pro Leu Phe Asp Ile Phe Asn Lys Val Lys Lys Glu Trp Asp

3515 3520 3525

Ser Phe Gln Asp Pro Val Ala Val Ser Phe Asp Thr Lys Ala Trp

3530 3535 3540

Asp Thr Gln Val Thr Ser Arg Asp Leu Met Leu Ile Lys Asp Ile

3545 3550 3555

Gln Lys Tyr Tyr Phe Lys Arg Ser Ile His Lys Phe Leu Asp Thr

3560 3565 3570

Ile Thr Glu His Met Val Glu Val Pro Val Ile Thr Ala Asp Gly

3575 3580 3585

Glu Val Tyr Ile Arg Asn Gly Gln Arg Gly Ser Gly Gln Pro Asp

3590 3595 3600

Thr Ser Ala Gly Asn Ser Met Leu Asn Val Leu Thr Met Ile Tyr

3605 3610 3615

Ala Phe Cys Lys Ser Thr Gly Ile Pro Tyr Arg Gly Phe Ser Arg

3620 3625 3630

Val Ala Arg IIe His Val Cys Gly Asp Asp Gly Phe Leu Ile Thr

3635 3640 3645

Glu Arg Gly Leu Gly Leu Lys Phe Ser Glu Lys Gly Met Gln Ile

3650 3655 3660

Leu His Glu Ala Gly Lys Pro Gln Lys Ile Thr Glu Gly Asp Lys

3665 3670 3675

Met Lys Val Ala Tyr Arg Phe Glu Asp Ile Glu Phe Cys Ser His

3680 3685 3690

Thr Pro Val Pro Val Arg Trp Ala Asp Asn Thr Ser Ser Tyr Met

3695 3700 3705

Ala Gly Arg Ser Thr Ala Thr Ile Leu Ala Lys Met Ala Thr Arg

3710 3715 3720

Leu Asp Ser Ser Gly Glu Arg Gly Ser Thr Ala Tyr Glu Lys Ala

3725 3730 3735

Val Ala Phe Ser Phe Leu Leu Met Tyr Ser Trp Asn Pro Val Val

3740 3745 3750

Arg Arg Ile Cys Leu Leu Val Leu Ser Gln Phe Pro Glu Ile Ser

3755 3760 3765

Pro Ser Lys Asn Thr Ile Tyr Tyr Tyr Gln Gly Asp Pro Ile Ala

3770 3775 3780

Ala Tyr Arg Glu Val Ile Gly Lys Gln Leu Cys Glu Leu Lys Arg

3785 3790 3795

Thr Gly Phe Glu Lys Leu Ala Gly Leu Asn Leu Ser Met Thr Thr

3800 3805 3810

Leu Gly Ile Trp Thr Lys His Thr Ser Lys Arg Leu Ile Gln Ala

3815 3820 3825

Cys Val Glu Ile Gly Lys Arg Glu Gly Thr Trp Leu Val Asn Ala

3830 3835 3840

Asp Arg Leu Ile Ala Gly Lys Thr Gly Lys Phe Tyr Ile Pro Ser

3845 3850 3855

Thr Gly Val Thr Leu Leu Gly Lys His Tyr Glu Glu Ile Asn Leu

3860 3865 3870

Lys Gln Lys Ala Ala Gln Pro Pro Ile Glu Gly Val Asp Arg Tyr

3875 3880 3885

Lys Leu Gly Pro Ile Val Asn Val Ile Leu Arg Arg Leu Arg Val

3890 3895 3900

Met Leu Met Thr Val Ala Ser Gly Ser Trp

3905 3910

<210>10

<211>12332

<212>DNA

<213>Artificial-序列：XIKE-C BVDV-序列

<400>10

gtatacgaga ttagctaaag aactcgtata tggattggac gtcaacaaat ttttaattgg 60

caacgtaggg aaccttcccc tcagcgaagg ccgaaaagag gctagccatg cccttagtag 120

gactagcaaa agtaggggac tagcggtagc agtgagttcg ttggatggcc gaacccctga 180

gtacagggga gtcgtcaatg gttcgacact ccattagtcg aggagtctcg agatgccatg 240

tggacgaggg catgcccacg gcacatctta acccatgcgg gggttgcatg ggtgaaagcg 300

ctattcgtgg cgttatggac acagcctgat agggtgtagc agagacctgc tattccgcta 360

gtaaaaactc tgctgtacat ggcacatgga gttgttttca aatgaacttt tatacaaaac 420

atataaacaa aaaccagcag gcgtcgtgga acctgtttac gacgtcaacg ggcgcccact 480

gtttggagag agcagtgact tgcacccgca gtcaacacta aaactaccac accaacgagg 540

cagcgccaac atcctgacca atgctaggtc cctaccgcgg aaaggtgact gccggagagg 600

taatgtgtat ggaccggtga gtggcatcta tatcaaacca ggaccgatct actaccagga 660

ttatgtgggc cccgtctatc atagagcccc actggaacta tgtagggagg caagtatgtg 720

cgaaacaact aggagagttg gcagagtgac cggtagtgat gggaaattat atcatatcta 780

catctgcata gatgggtgta tcctcctgaa gagggcgact aggaaccaac cagaagtcct 840

gaaatgggta tacaacagat taaattgtcc tttatgggtc accagctgct ccgatgaagg 900

gagcaagggt gctacaagta agaagcagcc taagccagat aggatagaaa aaggtaagat 960

gaaaatagcc ccaaaagaga cagaaaaaga ttgcaaaacc agaccccccg acgcgactat 1020

agtagtagaa ggggttaagt accaggtgaa gaaaaaagga aaggtaaggg gaaaaaatac 1080

tcaagatggg ttatatcaca acaagaataa gccccctgaa tcaagaaaaa aattggaaaa 1140

ggcactgctg gcttgggcca tcttagcagc ggtcctgctt cagctggtaa caggagagaa 1200

tatcacccag tggaacttga tggacaacgg caccgaggga atacagcaag cgatgttcct 1260

aagaggggtg aacaggagtc tattaggaat ttggccagag aaaatttgca ccggagtacc 1320

aactcactta gcaacagact atgagcttaa agagatagtg gggatgatgg acgcgagtga 1380

gaagaccaac tacacgtgtt gcaggttgca aagacatgag tggaataaac atggttggtg 1440

taactggttt catatagaac cgtggatatg gttgatgaac aaaacccaaa acaacctgac 1500

agaagggcaa ccgcttaggg agtgtgctgt gacttgtagg tatgacaagg aaacagaatt 1560

gaacatcgtg acacaggcta gggacagacc tacaactctg acaggttgca agaaaggcaa 1620

gaatttctct ttcgcaggtg ttatactgga tgggccctgt aactttaaag tatcggttga 1680

agatgtgctg ttcaaggagc acgattgcgg caacatgctg caagagaccg cgatacagct 1740

actcgatggg gcaaccaaca ccattgaggg agcaagggta gggacggcca agttgacaac 1800

ctggttaggg aagcaattag ggatccttgg taagaagttg gagaacaaaa gcaaagcatg 1860

gtttggtgca catgcagcaa gtccatactg cggagtggag aggaagatcg gttacgtatg 1920

gtatacaaaa aactgcactc cagcttgcct tccaagaaac actagaataa taggccccgg 1980

gaaatttgat accaacgccg aagatggaaa aatactccat gagatggggg ggcacctctc 2040

agaatttgtc ctattgtcct tggtggttct gtctgacttt gccccggaaa ccgcgagcgt 2100

catctacttg gttctacatt ttgcgatccc gcaaagccac gttgatgtag acacatgcga 2160

caagaaccag ctgaatttaa cggtagcaac cacagtagca gaggtcatac cagggacagt 2220

gtggaaccta gggaagtatg tctgcataag accagactgg tggccatatg agacgacgac 2280

agtcttcgtc atagaggaag cagggcaagt aatcaaattg atgctaaggg ccatcagaga 2340

cttaactagg atatggaatg ctgccactac cacagctttc ttaatctttt tagtaaaagc 2400

actgagggga caactaatcc aagggctatt gtggctgatg ctaataacag gagcacaggg 2460

cttccctgaa tgcaaagagg gcttccaata tgccatatct aaagacagga aaatggggtt 2520

attggggcca gagagcttaa ctacaacatg gcacctcccc accaaaaaaa tagtggattc 2580

catggtgcat gtatggtgtg aaggaaaaga cttgaaaata ttaaaaatgt gcacaaagga 2640

agagaggtat ctagtggctg tgcacgagag agccttatca accagtgccg agtttatgca 2700

gatcagtgat gggacaatag gcccagacgt gatagatatg cctgatgact ttgagtttgg 2760

actctgccct tgtgactcaa aaccagtgat aaagggcaaa tttaatgcca gcttactgaa 2820

tggaccagct ttccagatgg tatgcccaca ggggtggact ggtacaatag aatgcaccct 2880

agcgaaccaa gacaccttgg acacaactgt cattaggaca tatagaagaa ctaccccatt 2940

tcagcggaga aaatggtgta cctatgaaaa aataataggg gaagatatct atgaatgcat 3000

tctaggtgga aactggacat gcataaccgg tgaccatagc aggttgaaag acggacctat 3060

caagaagtgt aagtggtgtg gccatgactt cgtcaactca gaggggctac cacactaccc 3120

aataggcaag tgcatgctca tcaacgagag tgggtacagg tatgtagatg acacctcttg 3180

cgataggggt ggtgtagcca tagttccatc tggcaccgta aagtgtagaa taggtaacgt 3240

cacggtgcaa gttatcgcta ctaacaatga tctgggaccc atgccttgca gcccagctga 3300

agtgatagca agtgaaggac cagtggaaaa gactgcatgc acattcaact attcaaggac 3360

tctacctaat aagtattatg agccaaggga ccggtacttc caacaataca tgttaaaagg 3420

ggagtggcaa tattggttcg acctggattc tgtagaccac cacaaagact acttctcaga 3480

gttcataatc atagcagtgg tcgccttgtt gggtggtaag tacgtactgt ggctcttgat 3540

aacatacaca atactgtctg agcagatggc tatgggtgct ggagtgaata ctgaagagat 3600

agtcatgata ggcaatttgc tgacagacag tgatattgag gttgtggttt atttccttct 3660

tctgtactta atagttaaag aggaactggc gaggaaatgg attatactgg tataccacat 3720

ccttgtagcc aaccctatga aaacaattgg ggtcgtctta ctaatgctag ggggagtggt 3780

gaaggccagc agaatcaatg ctgatgacca aagtgctatg gacccatgct ttcttctcgt 3840

gacaggcgta gtggctgttt tgatgatcgc tagaagagaa cctgccacat taccactgat 3900

tgtagcattg ctagcaataa gaacatcagg attcctactg cccgctagca ttgatgtaac 3960

tgtagcagta gtattaattg tacttttgtt ggctagctac ataacagact actttagata 4020

taaaaagtgg cttcaactct tatttagtct gatagctggt atctttatta taaggagctt 4080

aaaacatatc aaccagatgg aggtaccaga aatatctatg ccaagttgga gacctctagc 4140

tctggtcctt ttctatataa catctacagc aataaccact aattgggaca ttgacttagc 4200

aggcttcctg ctgcaatggg cgccagcagt gatcatgatg gctaccatgt gggcagactt 4260

tttgactctg atcatagtcc tgcccagtta cgagttatct aagctttact tcctaaagaa 4320

cgtcaggaca gacgtggaaa agaactggct cggcaaagtg aaatacagac agatcagttc 4380

agtttatgac atctgtgaca gtgaggaagc agtgtaccta tttccatcaa ggcataagag 4440

tggaagcagg ccagatttca tattaccttt tttgaaagcc gtgttaataa gctgcatcag 4500

cagccaatgg caagtggttt acatttctta cctaatactg gaaattacat actatatgca 4560

caggaaaatc atagatgagg tgtcaggagg agcaaatttt ctatcaagac tcatagcagc 4620

catcatagaa ttaaattggg ccatagatga tgaggaatgt aaaggactga agaaactgta 4680

tctcttgtca gggagagcga agaatttgat agttaaacat aaggtaagaa atgaagccgt 4740

ccacagatgg tttggtgagg aggaaatata cggggcaccc aaggtgatca ctatcataaa 4800

agctagtacc ctaagtaaaa acaggcactg cataatctgc acgatctgtg aagggaaaga 4860

atggaatgga gccaactgcc caaagtgtgg aagacaagga aagcccataa catgtggaat 4920

gacactcgca gactttgagg agaaacatta caaaaagata tttataagag aagaatcttc 4980

ttgtcctgtg ccttttgatc cttcttgcca ttgtaattat tttcgccacg atgggccttt 5040

caggaaagag tataagggtt acgtccaata cacagccaga ggacaactct ttctgaggaa 5100

cctaccaatt ctagcgacga agatgaagct attaatggtg ggaaacctcg gcgcagaaat 5160

tggcgacctg gaacatctag gatgggtact gagagggcca gccgtgtgca aaaaaattac 5220

caaccatgag aagtgccacg taaacatcat ggataagcta actgcatttt ttggaatcat 5280

gcctagaggc acgaccccta gggcacctgt gaggttcccc acagcactac taaaagtgag 5340

aagggggcta gagacgggat gggcttacac gcaccaagga gggatcagct cggtagacca 5400

tgtcacagcc ggaaaggatt tactagtgtg tgacagtatg ggcaggacca gggttgtctg 5460

tcatagtaac aataagatga ctgatgagac tgagtatggc atcaagaccg actcagggtg 5520

tcccgaaggt gcgaggtgtt acgtgctaaa cccagaagct gttaacattt ctggcacaaa 5580

aggagctatg gtacacctcc agaaaacggg gggggagttc acatgtgtca ctgcctcagg 5640

gaccccggct ttcttcgatc tgaaaaatct aaaaggctgg tccgggctac caatttttga 5700

agcatccagt ggcagggtgg ttggtagggt gaaagtcggc aagaatgagg attccaagcc 5760

caccaaacta atgagcggaa tccagacagt gtctaagaac cagacagacc tagcggacat 5820

cgtaaaaaaa ttgactagta tgaacagagg agagttcaaa cagataacat tagccactgg 5880

ggcaggaaaa actacggaac tgccaaggtc cgtcatagag gagataggga ggcacaaaag 5940

ggtcttagtc ctgataccat tgagagcagc agcagagtca gtgtatcagt atatgagagt 6000

gaagtaccca agtatatctt tcaatttgag aataggagat atgaaggaag gtgacatggc 6060

cactggtatc acctacgcct catatgggta cttttgtcag cttcctcagc ccaaactgag 6120

agctgccatg gtagagtact catatatatt cttagatgag taccactgtg ctacacccga 6180

gcaattagca ataattggaa agatacacag gtttgctgaa aatcttagag tggtagcaat 6240

gacagcaacc ccagctggaa cggtcacaac gactggtcag aaacacccta tagaggagtt 6300

catagcccca gaggtgatga aaggtgaaga tctaggtagt gaatacttgg atattgcagg 6360

gttgaagata ccgactgaag agatgaaagg caacatgctc gtgttcgcgc caactaggaa 6420

catggcagta gaaacagcta agaaattgaa ggctaaggga tacaactctg gatactatta 6480

cagtggggaa aacccagaga acttgagggt ggtaacctcg caatccccgt atgtggtagt 6540

agccaccaat gccatagagt caggtgtgac attaccagac ttagacacag ttgtagacac 6600

tggactaaag tgtgagaaga gggtgaggat ttcttcaaaa atgcccttca ttgtaacagg 6660

acttaagaga atggcagtca caatcggaga gcaagcccag cgcaggggta gagtaggaag 6720

agtcaagcca ggtaggtact ataggagtca agaaacagct tcagggtcaa aagattacca 6780

ttacgaccta ctgcaagccc agaggtacgg aatagaagat ggaattaatg taacaaagtc 6840

attcagggag atgaactatg attggagcct ttacgaagag gacagcttga tgataactca 6900

actcgaggtc cttaacaacc tccttatatc agaagacctg cctgccgcag tgaagaacat 6960

catggcccgg accgatcacc cagaacccat acaactggcc tataacagtt atgaaaacca 7020

aattccagtg ctgttcccaa agatcaaaaa tggtgaggtg acagacagtt atgagaatta 7080

cacatatctc aatgcaagaa aattaggaga ggacgtgccg gcatatgtgt acgccacaga 7140

ggatgaggat ctagcagtgg atcttctggg tatggattgg ccggacccag gcaaccaaca 7200

ggtggtagag acagggaggg cattaaaaca agtaactggc ttatccacag cagaaaacgc 7260

cctcttgata gccctattcg gctacgtcgg gtaccagaca ctttcaaaaa ggcacatacc 7320

catgattact gacatctata cacttgaaga ccacaggctt gaggacacaa cccacctcca 7380

gtttgcccca aacgctataa ggaccgacgg caaggactca gagttgaagg aattagctgt 7440

gggagacctt gataaatatg tggacgcact ggtagactac tccaaacaag ggatgaaatt 7500

catcaaagtc caagctgaaa aggtcagaga ctcccagtct acgaaggaag gcttgcaaac 7560

cattaaggag tatgtggata agtttataca atcactaaca gagaataagg aggagatcat 7620

caggtatgga ctatggggag ttcacacggc actctacaaa agcttggcag cgagactggg 7680

gcatgaaaca gcttttgcaa ctttagtggt aaaatggttg gcttttgggg gcgaaacggt 7740

atctgctcac atcaagcaag tagcagttga tctagtagta tattatatca tcaacaaacc 7800

atcttttcct ggagatacag agacccaaca agaggggagg aagtttgtgg ctagtctttt 7860

tatatctgca ctagcaacat acacatataa aacctggaat tacaacaatc tgcaacgggt 7920

tgtcgaacct gccttagctt acctcccata tgctacaagt gccttgaagt tgttcacacc 7980

cacaagatta gagagtgtgg tcatactcag ttctacaatt tacaagacat acctctctat 8040

aaggaagggt aagagtgacg gcttgttagg tacaggcata agtgcagcca tggagatctt 8100

aaaccaaaac ccaatctcag taggtatatc tgtgatgctg ggggtaggtg ccatcgccgc 8160

ccataatgca atagaatcta gtgaacagaa aagaactttg ctgatgaagg tctttgtaaa 8220

aaacttctta gaccaagcag caacagatga gctagtcaaa gagaaccctg aaaaaataat 8280

catggctcta tttgaagcag tccagaccat aggaaacccc ctaagactca tctaccatct 8340

gtacggggtg tactataagg ggtgggaagc aaaagaactc gcagagaaaa ctgctggccg 8400

caacttattc acattgatca tgtttgaggc ctttgagctt ttaggtatgg actcagaagg 8460

aaagataaga aacttgtcag gcaactacat actggactta atcttcaact tgcataataa 8520

attaaacaag gggctcaaaa aactagtcct tgggtgggct cctgcacctt tgagctgtga 8580

ttggacacca agtgatgaga gaataagcct acctcataac aactacttaa gggtagaaac 8640

caggtgtcct tgtggctatg agatgaaggc aataaaaaat gttgctggta aattgacaaa 8700

agttgaagaa aaggggtcct tcctatgcag gaatagatta gggagaggac ctccaaactt 8760

caaagtaaca aagttctatg atgataactt gatagaagtc aagccagtag ctaggctaga 8820

aggccaggtg gacctctatt acaagggagt aacagctaag ttagactaca acaatgggaa 8880

agtactgtta gctaccaaca agtgggaggt ggaccacgct ttcctgacca gactagtaaa 8940

gaagcacaca gggataggtt ttaaaggtgc atatttgggt gaccgaccag accatcaaga 9000

tcttgtcgat agagattgtg caactataac gaagaactca gtacagttcc taaaaatgaa 9060

gaagggttgc gctttcacat atgacctaac aatctctaac cttgtcaggc ttattgaact 9120

agtccataag aataatttac aagaaagaga gatccctacc gtgacagtaa ctacttggct 9180

tgcatattct tttgtcaatg aagacctggg gactatcaag cctgtattgg gggagaaagt 9240

catcccagaa ccccccgagg agttgagtct ccaacccacc gtgagactag tcaccactga 9300

aacagcaata accataacag gggaggctga agtgatgacg acagggatca caccagtggt 9360

agagatgaaa gaagaacctc agctggacca ccagtcaact accctaaagg tagggttgaa 9420

ggaaggggaa tatccagggc caggagttaa ccctaaccat ttagcagagg tgatagatga 9480

gaaagatgac aggccttttg tcctaatcat cggtaacaaa ggttctacct cgaacagagc 9540

aagaacggcc aagaatatac ggctgtacaa aggaaacaac ccaagagaga tcagggatct 9600

gatgagccaa ggaagaatat tgacggttgc tctaaaagag ttggacccgg aattaaaaga 9660

attagtagat tacaagggga cctttctcaa tagggaagct ttagaagccc taagcttagg 9720

taagccaatc aagaggaaaa ccacaacagc aatgatcagg aggttaatag agccagaggt 9780

tgaggaggaa ctaccagatt ggttccaagc ggaagaaccc ctatttttgg aagcaaaaat 9840

acagaatgac ttataccacc taattggcag tgtagatagt ataaaaagca aagcaaagga 9900

attaggggcc acagataaca caaagatagt gaaggaagtt ggggctagga cctatacgat 9960

gaaattgagc agctggagca cacaagttac aaaaaaacag atgagtctag cccctctctt 10020

tgaagagctg ttattaaagt gccctccatg tagtaaaatt tcaaagggac atatggtgtc 10080

agcataccaa ctggctcaag gaaactggga acccctcggg tgtggggtct atatgggaac 10140

cataccagct aggcgtctca agatccaccc ttatgaggct taccttaaac tcaaagagct 10200

ggtggaagtt gaatcttcga gggccactgc aaaagaatcc atcataagag aacataacac 10260

ctggatcctg cggaaggtga gacatgaagg gaacctaaga accaaatcaa tgatcaaccc 10320

tgggaaaata tcagatcagc tatgcagaga tggacacaaa agaaacatat ataataagat 10380

cataggctca acaatggcct ctgctggtat taggctggag aaactgccag tagtccgagc 10440

ccaaactgac acaaccagtt tccaccaagc cataagagaa aaaattgata aaacagaaaa 10500

caagcagacc cctgaattgc atgaagaact aatgaaggtc ttcgactgct taaagatccc 10560

agagctgaag gaatcgtatg atgaagtttc atgggaacaa ttagaagccg ggataaaccg 10620

taagggtgca gcaggctatc tagagagcaa gaacataggg gaagtcctag acacagagaa 10680

acacatagta gagcagctga tcaaggatct gaggaagggg aagaagatta ggtactatga 10740

aacagccatc cccaagaatg agaagagaga cgtcagcgac gactgggaag ccggagagtt 10800

cgttgatgaa aagaaaccaa gagtaatcca gtacccggac gccaaggtga gactggccat 10860

tacaaaagtg atgtacaaat gggtaaagca aaaaccagtg gtgatacccg gctatgaagg 10920

taaaacacct ctatttgaca tattcaacaa agtgaagaag gaatgggatt cattccagga 10980

ccccgtagca gtgagctttg acaccaaagc gtgggataca caagtcacca gtagagacct 11040

aatgttgata aaggatatcc agaaatatta tttcaagaga agtatacaca aatttttaga 11100

tacaataaca gaacacatgg tggaggtacc tgtcattaca gcagacggtg aagtttacat 11160

aaggaatggt cagaggggta gtggccaacc cgacacaagt gctggtaata gtatgttgaa 11220

tgtcctaacc atgatatatg ctttctgtaa aagtacaggc ataccttaca ggggattcag 11280

cagagtggca agaatccatg tgtgtggtga tgatggcttt ttgataacag agagaggact 11340

gggactgaaa ttctctgaga agggtatgca gatattacat gaggccggga agccccagaa 11400

aataactgaa ggggacaaaa tgaaagtggc atacagattc gaggacatag agttttgttc 11460

ccatactccc gtgccagtca gatgggcaga taacaccagt agttacatgg cagggaggag 11520

cacagccact atactagcta agatggcaac caggctggat tccagcggag agaggggtag 11580

cacagcttat gagaaggccg tagccttcag cttccttttg atgtactcat ggaatcccgt 11640

agttagaagg atctgcttac tggtgttgtc acagtttcca gaaatatccc catccaaaaa 11700

cacaatatac tactaccaag gggatcccat agctgcgtac agagaagtga tagggaaaca 11760

gctgtgtgaa ctgaaaagaa caggatttga gaagctggct ggtctgaatt tgagtatgac 11820

cactctaggc atctggacaa aacatactag taaaagacta atccaagcct gtgtagaaat 11880

aggtaagaga gaaggtacct ggttagttaa tgctgacaga ctgattgcag gaaagactgg 11940

gaagttttac atcccaagca ctggtgtcac tctgttggga aaacactatg aggaaattaa 12000

cttaaagcaa aaggcggcac aaccgccgat agagggggtt gacagatata agttgggccc 12060

catagttaat gttatcttga gaaggctgag ggtgatgctg atgacagttg ccagcggaag 12120

ctggtgaatc cgtccggagc gtcgtgccct cactcaaggt ttttaattgt aaatattgta 12180

aatagacagc taagatattt attgtagttg gatagtaatg cagtgatagt aaatacccca 12240

atttaacact acctccaatg cactaagcac tttagctgtg tgaggttaac tcgacgtcca 12300

cggttggact agggaagacc tctaacagcc cc 12332

<210>11

<211>11840

<212>DNA

<213>人工序列：XIKE-C-NdN BVDV-序列

<400>11

gtatacgaga ttagctaaag aactcgtata tggattggac gtcaacaaat ttttaattgg 60

caacgtaggg aaccttcccc tcagcgaagg ccgaaaagag gctagccatg cccttagtag 120

gactagcaaa agtaggggac tagcggtagc agtgagttcg ttggatggcc gaacccctga 180

gtacagggga gtcgtcaatg gttcgacact ccattagtcg aggagtctcg agatgccatg 240

tggacgaggg catgcccacg gcacatctta acccatgcgg gggttgcatg ggtgaaagcg 300

ctattcgtgg cgttatggac acagcctgat agggtgtagc agagacctgc tattccgcta 360

gtaaaaactc tgctgtacat ggcacatgga gttgttttcc gatgaaggga gcaagggtgc 420

tacaagtaag aagcagccta agccagatag gatagaaaaa ggtaagatga aaatagcccc 480

aaaagagaca gaaaaagatt gcaaaaccag accccccgac gcgactatag tagtagaagg 540

ggttaagtac caggtgaaga aaaaaggaaa ggtaagggga aaaaatactc aagatgggtt 600

atatcacaac aagaataagc cccctgaatc aagaaaaaaa ttggaaaagg cactgctggc 660

ttgggccatc ttagcagcgg tcctgcttca gctggtaaca ggagagaata tcacccagtg 720

gaacttgatg gacaacggca ccgagggaat acagcaagcg atgttcctaa gaggggtgaa 780

caggagtcta ttaggaattt ggccagagaa aatttgcacc ggagtaccaa ctcacttagc 840

aacagactat gagcttaaag agatagtggg gatgatggac gcgagtgaga agaccaacta 900

cacgtgttgc aggttgcaaa gacatgagtg gaataaacat ggttggtgta actggtttca 960

tatagaaccg tggatatggt tgatgaacaa aacccaaaac aacctgacag aagggcaacc 1020

gcttagggag tgtgctgtga cttgtaggta tgacaaggaa acagaattga acatcgtgac 1080

acaggctagg gacagaccta caactctgac aggttgcaag aaaggcaaga atttctcttt 1140

cgcaggtgtt atactggatg ggccctgtaa ctttaaagta tcggttgaag atgtgctgtt 1200

caaggagcac gattgcggca acatgctgca agagaccgcg atacagctac tcgatggggc 1260

aaccaacacc attgagggag caagggtagg gacggccaag ttgacaacct ggttagggaa 1320

gcaattaggg atccttggta agaagttgga gaacaaaagc aaagcatggt ttggtgcaca 1380

tgcagcaagt ccatactgcg gagtggagag gaagatcggt tacgtatggt atacaaaaaa 1440

ctgcactcca gcttgccttc caagaaacac tagaataata ggccccggga aatttgatac 1500

caacgccgaa gatggaaaaa tactccatga gatggggggg cacctctcag aatttgtcct 1560

attgtccttg gtggttctgt ctgactttgc cccggaaacc gcgagcgtca tctacttggt 1620

tctacatttt gGgatcccgc aaagccacgt tgatgtagac acatgcgaca agaaccagct 1680

gaatttaacg gtagcaacca cagtagcaga ggtcatacca gggacagtgt ggaacctagg 1740

gaagtatgtc tgcataagac cagactggtg gccatatgag acgacgacag tcttcgtcat 1800

agaggaagca gggcaagtaa tcaaattgat gctaagggcc atcagagact taactaggat 1860

atggaatgct gccactacca cagctttctt aatcttttta gtaaaagcac tgaggggaca 1920

actaatccaa gggctattgt ggctgatgct aataacagga gcacagggct tccctgaatg 1980

caaagagggc ttccaatatg ccatatctaa agacaggaaa atggggttat tggggccaga 2040

gagcttaact acaacatggc acctccccac caaaaaaata gtggattcca tggtgcatgt 2100

atggtgtgaa ggaaaagact tgaaaatatt aaaaatgtgc acaaaggaag agaggtatct 2160

agtggctgtg cacgagagag ccttatcaac cagtgccgag tttatgcaga tcagtgatgg 2220

gacaataggc ccagacgtga tagatatgcc tgatgacttt gagtttggac tctgcccttg 2280

tgactcaaaa ccagtgataa agggcaaatt taatgccagc ttactgaatg gaccagcttt 2340

ccagatggta tgcccacagg ggtggactgg tacaatagaa tgcaccctag cgaaccaaga 2400

caccttggac acaactgtca ttaggacata tagaagaact accccatttc agcggagaaa 2460

atggtgtacc tatgaaaaaa taatagggga agatatctat gaatgcattc taggtggaaa 2520

ctggacatgc ataaccggtg accatagcag gttgaaagac ggacctatca agaagtgtaa 2580

gtggtgtggc catgacttcg tcaactcaga ggggctacca cactacccaa taggcaagtg 2640

catgctcatc aacgagagtg ggtacaggta tgtagatgac acctcttgcg ataggggtgg 2700

tgtagccata gttccatctg gcaccgtaaa gtgtagaata ggtaacgtca cggtgcaagt 2760

tatcgctact aacaatgatc tgggacccat gccttgcagc ccagctgaag tgatagcaag 2820

tgaaggacca gtggaaaaga ctgcatgcac attcaactat tcaaggactc tacctaataa 2880

gtattatgag ccaagggacc ggtacttcca acaatacatg ttaaaagggg agtggcaata 2940

ttggttcgac ctggattctg tagaccacca caaagactac ttctcagagt tcataatcat 3000

agcagtggtc gccttgttgg gtggtaagta cgtactgtgg ctcttgataa catacacaat 3060

actgtctgag cagatggcta tgggtgctgg agtgaatact gaagagatag tcatgatagg 3120

caatttgctg acagacagtg atattgaggt tgtggtttat ttccttcttc tgtacttaat 3180

agttaaagag gaactggcga ggaaatggat tatactggta taccacatcc ttgtagccaa 3240

ccctatgaaa acaattgggg tcgtcttact aatgctaggg ggagtggtga aggccagcag 3300

aatcaatgct gatgaccaaa gtgctatgga cccatgcttt cttctcgtga caggcgtagt 3360

ggctgttttg atgatcgcta gaagagaacc tgccacatta ccactgattg tagcattgct 3420

agcaataaga acatcaggat tcctactgcc cgctagcatt gatgtaactg tagcagtagt 3480

attaattgta cttttgttgg ctagctacat aacagactac tttagatata aaaagtggct 3540

tcaactctta tttagtctga tagctggtat ctttattata aggagcttaa aacatatcaa 3600

ccagatggag gtaccagaaa tatctatgcc aagttggaga cctctagctc tggtcctttt 3660

ctatataaca tctacagcaa taaccactaa ttgggacatt gacttagcag gcttcctgct 3720

gcaatgggcg ccagcagtga tcatgatggc taccatgtgg gcagactttt tgactctgat 3780

catagtcctg cccagttacg agttatctaa gctttacttc ctaaagaacg tcaggacaga 3840

cgtggaaaag aactggctcg gcaaagtgaa atacagacag atcagttcag tttatgacat 3900

ctgtgacagt gaggaagcag tgtacctatt tccatcaagg cataagagtg gaagcaggcc 3960

agatttcata ttaccttttt tgaaagccgt gttaataagc tgcatcagca gccaatggca 4020

agtggtttac atttcttacc taatactgga aattacatac tatatgcaca ggaaaatcat 4080

agatgaggtg tcaggaggag caaattttct atcaagactc atagcagcca tcatagaatt 4140

aaattgggcc atagatgatg aggaatgtaa aggactgaag aaactgtatc tcttgtcagg 4200

gagagcgaag aatttgatag ttaaacataa ggtaagaaat gaagccgtcc acagatggtt 4260

tggtgaggag gaaatatacg gggcacccaa ggtgatcact atcataaaag ctagtaccct 4320

aagtaaaaac aggcactgca taatctgcac gatctgtgaa gggaaagaat ggaatggagc 4380

caactgccca aagtgtggaa gacaaggaaa gcccataaca tgtggaatga cactcgcaga 4440

ctttgaggag aaacattaca aaaagatatt tataagagaa gaatcttctt gtcctgtgcc 4500

ttttgatcct tcttgccatt gtaattattt tcgccacgat gggcctttca ggaaagagta 4560

taagggttac gtccaataca cagccagagg acaactcttt ctgaggaacc taccaattct 4620

agcgacgaag atgaagctat taatggtggg aaacctcggc gcagaaattg gcgacctgga 4680

acatctagga tgggtactga gagggccagc cgtgtgcaaa aaaattacca accatgagaa 4740

gtgccacgta aacatcatgg ataagctaac tgcatttttt ggaatcatgc ctagaggcac 4800

gacccctagg gcacctgtga ggttccccac agcactacta aaagtgagaa gggggctaga 4860

gacgggatgg gcttacacgc accaaggagg gatcagctcg gtagaccatg tcacagccgg 4920

aaaggattta ctagtgtgtg acagtatggg caggaccagg gttgtctgtc atagtaacaa 4980

taagatgact gatgagactg agtatggcat caagaccgac tcagggtgtc ccgaaggtgc 5040

gaggtgttac gtgctaaacc cagaagctgt taacatttct ggcacaaaag gagctatggt 5100

acacctccag aaaacggggg gggagttcac atgtgtcact gcctcaggga ccccggcttt 5160

cttcgatctg aaaaatctaa aaggctggtc cgggctacca atttttgaag catccagtgg 5220

cagggtggtt ggtagggtga aagtcggcaa gaatgaggat tccaagccca ccaaactaat 5280

gagcggaatc cagacagtgt ctaagaacca gacagaccta gcggacatcg taaaaaaatt 5340

gactagtatg aacagaggag agttcaaaca gataacatta gccactgggg caggaaaaac 5400

tacggaactg ccaaggtccg tcatagagga gatagggagg cacaaaaggg tcttagtcct 5460

gataccattg agagcagcag cagagtcagt gtatcagtat atgagagtga agtacccaag 5520

tatatctttc aatttgagaa taggagatat gaaggaaggt gacatggcca ctggtatcac 5580

ctacgcctca tatgggtact tttgtcagct tcctcagccc aaactgagag ctgccatggt 5640

agagtactca tatatattct tagatgagta ccactgtgct acacccgagc aattagcaat 5700

aattggaaag atacacaggt ttgctgaaaa tcttagagtg gtagcaatga cagcaacccc 5760

agctggaacg gtcacaacga ctggtcagaa acaccctata gaggagttca tagccccaga 5820

ggtgatgaaa ggtgaagatc taggtagtga atacttggat attgcagggt tgaagatacc 5880

gactgaagag atgaaaggca acatgctcgt gttcgcgcca actaggaaca tggcagtaga 5940

aacagctaag aaattgaagg ctaagggata caactctgga tactattaca gtggggaaaa 6000

cccagagaac ttgagggtgg taacctcgca atccccgtat gtggtagtag ccaccaatgc 6060

catagagtca ggtgtgacat taccagactt agacacagtt gtagacactg gactaaagtg 6120

tgagaagagg gtgaggattt cttcaaaaat gcccttcatt gtaacaggac ttaagagaat 6180

ggcagtcaca atcggagagc aagcccagcg caggggtaga gtaggaagag tcaagccagg 6240

taggtactat aggagtcaag aaacagcttc agggtcaaaa gattaccatt acgacctact 6300

gcaagcccag aggtacggaa tagaagatgg aattaatgta acaaagtcat tcagggagat 6360

gaactatgat tggagccttt acgaagagga cagcttgatg ataactcaac tcgaggtcct 6420

taacaacctc cttatatcag aagacctgcc tgccgcagtg aagaacatca tggcccggac 6480

cgatcaccca gaacccatac aactggccta taacagttat gaaaaccaaa ttccagtgct 6540

gttcccaaag atcaaaaatg gtgaggtgac agacagttat gagaattaca catatctcaa 6600

tgcaagaaaa ttaggagagg acgtgccggc atatgtgtac gccacagagg atgaggatct 6660

agcagtggat cttctgggta tggattggcc ggacccaggc aaccaacagg tggtagagac 6720

agggagggca ttaaaacaag taactggctt atccacagca gaaaacgccc tcttgatagc 6780

cctattcggc tacgtcgggt accagacact ttcaaaaagg cacataccca tgattactga 6840

catctataca cttgaagacc acaggcttga ggacacaacc cacctccagt ttgccccaaa 6900

cgctataagg accgacggca aggactcaga gttgaaggaa ttagctgtgg gagaccttga 6960

taaatatgtg gacgcactgg tagactactc caaacaaggg atgaaattca tcaaagtcca 7020

agctgaaaag gtcagagact cccagtctac gaaggaaggc ttgcaaacca ttaaggagta 7080

tgtggataag tttatacaat cactaacaga gaataaggag gagatcatca ggtatggact 7140

atggggagtt cacacggcac tctacaaaag cttggcagcg agactggggc atgaaacagc 7200

ttttgcaact ttagtggtaa aatggttggc ttttgggggc gaaacggtat ctgctcacat 7260

caagcaagta gcagttgatc tagtagtata ttatatcatc aacaaaccat cttttcctgg 7320

agatacagag acccaacaag aggggaggaa gtttgtggct agtcttttta tatctgcact 7380

agcaacatac acatataaaa cctggaatta caacaatctg caacgggttg tcgaacctgc 7440

cttagcttac ctcccatatg ctacaagtgc cttgaagttg ttcacaccca caagattaga 7500

gagtgtggtc atactcagtt ctacaattta caagacatac ctctctataa ggaagggtaa 7560

gagtgacggc ttgttaggta caggcataag tgcagccatg gagatcttaa accaaaaccc 7620

aatctcagta ggtatatctg tgatgctggg ggtaggtgcc atcgccgccc ataatgcaat 7680

agaatctagt gaacagaaaa gaactttgct gatgaaggtc tttgtaaaaa acttcttaga 7740

ccaagcagca acagatgagc tagtcaaaga gaaccctgaa aaaataatca tggctctatt 7800

tgaagcagtc cagaccatag gaaaccccct aagactcatc taccatctgt acggggtgta 7860

ctataagggg tgggaagcaa aagaactcgc agagaaaact gctggccgca acttattcac 7920

attgatcatg tttgaggcct ttgagctttt aggtatggac tcagaaggaa agataagaaa 7980

cttgtcaggc aactacatac tggacttaat cttcaacttg cataataaat taaacaaggg 8040

gctcaaaaaa ctagtccttg ggtgggctcc tgcacctttg agctgtgatt ggacaccaag 8100

tgatgagaga ataagcctac ctcataacaa ctacttaagg gtagaaacca ggtgtccttg 8160

tggctatgag atgaaggcaa taaaaaatgt tgctggtaaa ttgacaaaag ttgaagaaaa 8220

ggggtccttc ctatgcagga atagattagg gagaggacct ccaaacttca aagtaacaaa 8280

gttctatgat gataacttga tagaagtcaa gccagtagct aggctagaag gccaggtgga 8340

cctctattac aagggagtaa cagctaagtt agactacaac aatgggaaag tactgttagc 8400

taccaacaag tgggaggtgg accacgcttt cctgaccaga ctagtaaaga agcacacagg 8460

gataggtttt aaaggtgcat atttgggtga ccgaccagac catcaagatc ttgtcgatag 8520

agattgtgca actataacga agaactcagt acagttccta aaaatgaaga agggttgcgc 8580

tttcacatat gacctaacaa tctctaacct tgtcaggctt attgaactag tccataagaa 8640

taatttacaa gaaagagaga tccctaccgt gacagtaact acttggcttg catattcttt 8700

tgtcaatgaa gacctgggga ctatcaagcc tgtattgggg gagaaagtca tcccagaacc 8760

ccccgaggag ttgagtctcc aacccaccgt gagactagtc accactgaaa cagcaataac 8820

cataacaggg gaggctgaag tgatgacgac agggatcaca ccagtggtag agatgaaaga 8880

agaacctcag ctggaccacc agtcaactac cctaaaggta gggttgaagg aaggggaata 8940

tccagggcca ggagttaacc ctaaccattt agcagaggtg atagatgaga aagatgacag 9000

gccttttgtc ctaatcatcg gtaacaaagg ttctacctcg aacagagcaa gaacggccaa 9060

gaatatacgg ctgtacaaag gaaacaaccc aagagagatc agggatctga tgagccaagg 9120

aagaatattg acggttgctc taaaagagtt ggacccggaa ttaaaagaat tagtagatta 9180

caaggggacc tttctcaata gggaagcttt agaagcccta agcttaggta agccaatcaa 9240

gaggaaaacc acaacagcaa tgatcaggag gttaatagag ccagaggttg aggaggaact 9300

accagattgg ttccaagcgg aagaacccct atttttggaa gcaaaaatac agaatgactt 9360

ataccaccta attggcagtg tagatagtat aaaaagcaaa gcaaaggaat taggggccac 9420

agataacaca aagatagtga aggaagttgg ggctaggacc tatacgatga aattgagcag 9480

ctggagcaca caagttacaa aaaaacagat gagtctagcc cctctctttg aagagctgtt 9540

attaaagtgc cctccatgta gtaaaatttc aaagggacat atggtgtcag cataccaact 9600

ggctcaagga aactgggaac ccctcgggtg tggggtctat atgggaacca taccagctag 9660

gcgtctcaag atccaccctt atgaggctta ccttaaactc aaagagctgg tggaagttga 9720

atcttcgagg gccactgcaa aagaatccat cataagagaa cataacacct ggatcctgcg 9780

gaaggtgaga catgaaggga acctaagaac caaatcaatg atcaaccctg ggaaaatatc 9840

agatcagcta tgcagagatg gacacaaaag aaacatatat aataagatca taggctcaac 9900

aatggcctct gctggtatta ggctggagaa actgccagta gtccgagccc aaactgacac 9960

aaccagtttc caccaagcca taagagaaaa aattgataaa acagaaaaca agcagacccc 10020

tgaattgcat gaagaactaa tgaaggtctt cgactgctta aagatcccag agctgaagga 10080

atcgtatgat gaagtttcat gggaacaatt agaagccggg ataaaccgta agggtgcagc 10140

aggctatcta gagagcaaga acatagggga agtcctagac acagagaaac acatagtaga 10200

gcagctgatc aaggatctga ggaaggggaa gaagattagg tactatgaaa cagccatccc 10260

caagaatgag aagagagacg tcagcgacga ctgggaagcc ggagagttcg ttgatgaaaa 10320

gaaaccaaga gtaatccagt acccggacgc caaggtgaga ctggccatta caaaagtgat 10380

gtacaaatgg gtaaagcaaa aaccagtggt gatacccggc tatgaaggta aaacacctct 10440

atttgacata ttcaacaaag tgaagaagga atgggattca ttccaggacc ccgtagcagt 10500

gagctttgac accaaagcgt gggatacaca agtcaccagt agagacctaa tgttgataaa 10560

ggatatccag aaatattatt tcaagagaag tatacacaaa tttttagata caataacaga 10620

acacatggtg gaggtacctg tcattacagc agacggtgaa gtttacataa ggaatggtca 10680

gaggggtagt ggccaacccg acacaagtgc tggtaatagt atgttgaatg tcctaaccat 10740

gatatatgct ttctgtaaaa gtacaggcat accttacagg ggattcagca gagtggcaag 10800

aatccatgtg tgtggtgatg atggcttttt gataacagag agaggactgg gactgaaatt 10860

ctctgagaag ggtatgcaga tattacatga ggccgggaag ccccagaaaa taactgaagg 10920

ggacaaaatg aaagtggcat acagattcga ggacatagag ttttgttccc atactcccgt 10980

gccagtcaga tgggcagata acaccagtag ttacatggca gggaggagca cagccactat 11040

actagctaag atggcaacca ggctggattc cagcggagag aggggtagca cagcttatga 11100

gaaggccgta gccttcagct tccttttgat gtactcatgg aatcccgtag ttagaaggat 11160

ctgcttactg gtgttgtcac agtttccaga aatatcccca tccaaaaaca caatatacta 11220

ctaccaaggg gatcccatag ctgcgtacag agaagtgata gggaaacagc tgtgtgaact 11280

gaaaagaaca ggatttgaga agctggctgg tctgaatttg agtatgacca ctctaggcat 11340

ctggacaaaa catactagta aaagactaat ccaagcctgt gtagaaatag gtaagagaga 11400

aggtacctgg ttagttaatg ctgacagact gattgcagga aagactggga agttttacat 11460

cccaagcact ggtgtcactc tgttgggaaa acactatgag gaaattaact taaagcaaaa 11520

ggcggcacaa ccgccgatag agggggttga cagatataag ttgggcccca tagttaatgt 11580

tatcttgaga aggctgaggg tgatgctgat gacagttgcc agcggaagct ggtgaatccg 11640

tccggagcgt cgtgccctca ctcaaggttt ttaattgtaa atattgtaaa tagacagcta 11700

agatatttat tgtagttgga tagtaatgca gtgatagtaa ataccccaat ttaacactac 11760

ctccaatgca ctaagcactt tagctgtgtg aggttaactc gacgtccacg gttggactag 11820

ggaagacctc taacagcccc 11840

<210>12

<211>3749

<212>PRT

<213>人工序列：XIKE-C-NdN

<400>12

Met Glu Leu Phe Ser Asp Glu Gly Ser Lys Gly Ala Thr Ser Lys Lys

1 5 10 15

Gln Pro Lys Pro Asp Arg Ile Glu Lys Gly Lys Met Lys Ile Ala Pro

20 25 30

Lys Glu Thr Glu Lys Asp Cys Lys Thr Arg Pro Pro Asp Ala Thr Ile

35 40 45

Val Val Glu Gly Val Lys Tyr Gln Val Lys Lys Lys Gly Lys Val Arg

50 55 60

Gly Lys Asn Thr Gln Asp Gly Leu Tyr His Asn Lys Asn Lys Pro Pro

65 70 75 80

Glu Ser Arg Lys Lys Leu Glu Lys Ala Leu Leu Ala Trp Ala Ile Leu

85 90 95

Ala Ala Val Leu Leu Gln Leu Val Thr Gly Glu Asn Ile Thr Gln Trp

100 105 110

Asn Leu Met Asp Asn Gly Thr Glu Gly Ile Gln Gln Ala Met Phe Leu

115 120 125

Arg Gly Val Asn Arg Ser Leu Leu Gly Ile Trp Pro Glu Lys Ile Cys

130 135 140

Thr Gly Val Pro Thr His Leu Ala Thr Asp Tyr Glu Leu Lys Glu Ile

145 150 155 160

Val Gly Met Met Asp Ala Ser Glu Lys Thr Asn Tyr Thr Cys Cys Arg

165 170 175

Leu Gln Arg His Glu Trp Asn Lys His Gly Trp Cys Asn Trp Phe His

180 185 190

Ile Glu Pro Trp Ile Trp Leu Met Asn Lys Thr Gln Asn Asn Leu Thr

195 200 205

Glu Gly Gln Pro Leu Arg Glu Cys Ala Val Thr Cys Arg Tyr Asp Lys

210 215 220

Glu Thr Glu Leu Asn Ile Val Thr Gln Ala Arg Asp Arg Pro Thr Thr

225 230 235 240

Leu Thr Gly Cys Lys Lys Gly Lys Asn Phe Ser Phe Ala Gly Val Ile

245 250 255

Leu Asp Gly Pro Cys Asn Phe Lys Val Ser Val Glu Asp Val Leu Phe

260 265 270

Lys Glu His Asp Cys Gly Asn Met Leu Gln Glu Thr Ala Ile Gln Leu

275 280 285

Leu Asp Gly Ala Thr Asn Thr Ile Glu Gly Ala Arg Val Gly Thr Ala

290 295 300

Lys Leu Thr Thr Trp Leu Gly Lys Gln Leu Gly Ile Leu Gly Lys Lys

305 310 315 320

Leu Glu Asn Lys Ser Lys Ala Trp Phe Gly Ala His Ala Ala Ser Pro

325 330 335

Tyr Cys Gly Val Glu Arg Lys Ile Gly Tyr Val Trp Tyr Thr Lys Asn

340 345 350

Cys Thr Pro Ala Cys Leu Pro Arg Asn Thr Arg Ile Ile Gly Pro Gly

355 360 365

Lys Phe Asp Thr Asn Ala Glu Asp Gly Lys Ile Leu His Glu Met Gly

370 375 380

Gly His Leu Ser Glu Phe Val Leu Leu Ser Leu Val Val Leu Ser Asp

385 390 395 400

Phe Ala Pro Glu Thr Ala Ser Val Ile Tyr Leu Val Leu His Phe Ala

405 410 415

Ile Pro Gln Ser His Val Asp Val Asp Thr Cys Asp Lys Asn Gln Leu

420 425 430

Asn Leu Thr Val Ala Thr Thr Val Ala Glu Val Ile Pro Gly Thr Val

435 440 445

Trp Asn Leu Gly Lys Tyr Val Cys Ile Arg Pro Asp Trp Trp Pro Tyr

450 455 460

Glu Thr Thr Thr Val Phe Val Ile Glu Glu Ala Gly Gln Val Ile Lys

465 470 475 480

Leu Met Leu Arg Ala Ile Arg Asp Leu Thr Arg Ile Trp Asn Ala Ala

485 490 495

Thr Thr Thr Ala Phe Leu Ile Phe Leu Val Lys Ala Leu Arg Gly Gln

500 505 510

Leu Ile Gln Gly Leu Leu Trp Leu Met Leu Ile Thr Gly Ala Gln Gly

515 520 525

Phe Pro Glu Cys Lys Glu Gly Phe Gln Tyr Ala Ile Ser Lys Asp Arg

530 535 540

Lys Met Gly Leu Leu Gly Pro Glu Ser Leu Thr Thr Thr Trp His Leu

545 550 555 560

Pro Thr Lys Lys Ile Val Asp Ser Met Val His Val Trp Cys Glu Gly

565 570 575

Lys Asp Leu Lys Ile Leu Lys Met Cys Thr Lys Glu Glu Arg Tyr Leu

580 585 590

Val Ala Val His Glu Arg Ala Leu Ser Thr Ser Ala Glu Phe Met Gln

595 600 605

Ile Ser Asp Gly Thr Ile Gly Pro Asp Val Ile Asp Met Pro Asp Asp

610 615 620

Phe Glu Phe Gly Leu Cys Pro Cys Asp Ser Lys Pro Val Ile Lys Gly

625 630 635 640

Lys Phe Asn Ala Ser Leu Leu Asn Gly Pro Ala Phe Gln Met Val Cys

645 650 655

Pro Gln Gly Trp Thr Gly Thr Ile Glu Cys Thr Leu Ala Asn Gln Asp

660 665 670

Thr Leu Asp Thr Thr Val Ile Arg Thr Tyr Arg Arg Thr Thr Pro Phe

675 680 685

Gln Arg Arg Lys Trp Cys Thr Tyr Glu Lys Ile Ile Gly Glu Asp Ile

690 695 700

Tyr Glu Cys Ile Leu Gly Gly Asn Trp Thr Cys Ile Thr Gly Asp His

705 710 715 720

Ser Arg Leu Lys Asp Gly Pro Ile Lys Lys Cys Lys Trp Cys Gly His

725 730 735

Asp Phe Val Asn Ser Glu Gly Leu Pro His Tyr Pro Ile Gly Lys Cys

740 745 750

Met Leu Ile Asn Glu Ser Gly Tyr Arg Tyr Val Asp Asp Thr Ser Cys

755 760 765

Asp Arg Gly Gly Val Ala Ile Val Pro Ser Gly Thr Val Lys Cys Arg

770 775 780

Ile Gly Asn Val Thr Val Gln Val Ile Ala Thr Asn Asn Asp Leu Gly

785 790 795 800

Pro Met Pro Cys Ser Pro Ala Glu Val Ile Ala Ser Glu Gly Pro Val

805 810 815

Glu Lys Thr Ala Cys Thr Phe Asn Tyr Ser Arg Thr Leu Pro Asn Lys

820 825 830

Tyr Tyr Glu Pro Arg Asp Arg Tyr Phe Gln Gln Tyr Met Leu Lys Gly

835 840 845

Glu Trp Gln Tyr Trp Phe Asp Leu Asp Ser Val Asp His His Lys Asp

850 855 860

Tyr Phe Ser Glu Phe Ile Ile Ile Ala Val Val Ala Leu Leu Gly Gly

865 870 875 880

Lys Tyr Val Leu Trp Leu Leu Ile Thr Tyr Thr Ile Leu Ser Glu Gln

885 890 895

Met Ala Met Gly Ala Gly Val Asn Thr Glu Glu Ile Val Met Ile Gly

900 905 910

Asn Leu Leu Thr Asp Ser Asp Ile Glu Val Val Val Tyr Phe Leu Leu

915 920 925

Leu Tyr Leu Ile Val Lys Glu Glu Leu Ala Arg Lys Trp Ile Ile Leu

930 935 940

Val Tyr His Ile Leu Val Ala Asn Pro Met Lys Thr Ile Gly Val Val

945 950 955 960

Leu Leu Met Leu Gly Gly Val Val Lys Ala Ser Arg Ile Asn Ala Asp

965 970 975

Asp Gln Ser Ala Met Asp Pro Cys Phe Leu Leu Val Thr Gly Val Val

980 985 990

Ala Val Leu Met Ile Ala Arg Arg Glu Pro Ala Thr Leu Pro Leu Ile

995 1000 1005

Val Ala Leu Leu Ala Ile Arg Thr Ser Gly Phe Leu Leu Pro Ala

1010 1015 1020

Ser Ile Asp Val Thr Val Ala Val Val Leu Ile Val Leu Leu Leu

1025 1030 1035

Ala Ser Tyr Ile Thr Asp Tyr Phe Arg Tyr Lys Lys Trp Leu Gln

1040 1045 1050

Leu Leu Phe Ser Leu Ile Ala Gly Ile Phe Ile Ile Arg Ser Leu

1055 1060 1065

Lys His Ile Asn Gln Met Glu Val Pro Glu Ile Ser Met Pro Ser

1070 1075 1080

Trp Arg Pro Leu Ala Leu Val Leu Phe Tyr Ile Thr Ser Thr Ala

1085 1090 1095

Ile Thr Thr Asn Trp Asp Ile Asp Leu Ala Gly Phe Leu Leu Gln

1100 1105 1110

Trp Ala Pro Ala Val Ile Met Met Ala Thr Met Trp Ala Asp Phe

1115 1120 1125

Leu Thr Leu Ile Ile Val Leu Pro Ser Tyr Glu Leu Ser Lys Leu

1130 1135 1140

Tyr Phe Leu Lys Asn Val Arg Thr Asp Val Glu Lys Asn Trp Leu

1145 1150 1155

Gly Lys Val Lys Tyr Arg Gln Ile Ser Ser Val Tyr Asp Ile Cys

1160 1165 1170

Asp Ser Glu Glu Ala Val Tyr Leu Phe Pro Ser Arg His Lys Ser

1175 1180 1185

Gly Ser Arg Pro Asp Phe Ile Leu Pro Phe Leu Lys Ala Val Leu

1190 1195 1200

Ile Ser Cys Ilc Ser Ser Gln Trp Gln Val Val Tyr Ile Ser Tyr

1205 1210 1215

Leu Ile Leu Glu Ile Thr Tyr Tyr Met His Arg Lys Ile Ile Asp

1220 1225 1230

Glu Val Ser Gly Gly Ala Asn Phe Leu Ser Arg Leu Ile Ala Ala

1235 1240 1245

Ile Ile Glu Leu Asn Trp Ala Ile Asp Asp Glu Glu Cys Lys Gly

1250 1255 1260

Leu Lys Lys Leu Tyr Leu Leu Ser Gly Arg Ala Lys Asn Leu Ile

1265 1270 1275

Val Lys His Lys Val Arg Asn Glu Ala Val His Arg Trp Phe Gly

1280 1285 1290

Glu Glu Glu Ile Tyr Gly Ala Pro Lys Val Ile Thr Ile Ile Lys

1295 1300 1305

Ala Ser Thr Leu Ser Lys Asn Arg His Cys Ile Ile Cys Thr Ile

1310 1315 1320

Cys Glu Gly Lys Glu Trp Asn Gly Ala Asn Cys Pro Lys Cys Gly

1325 1330 1335

Arg Gln Gly Lys Pro Ile Thr Cys Gly Met Thr Leu Ala Asp Phe

1340 1345 1350

Glu Glu Lys His Tyr Lys Lys Ile Phe Ile Arg Glu Glu Ser Ser

1355 1360 1365

Cys Pro Val Pro Phe Asp Pro Ser Cys His Cys Asn Tyr Phe Arg

1370 1375 1380

His Asp Gly Pro Phe Arg Lys Glu Tyr Lys Gly Tyr Val Gln Tyr

1385 1390 1395

Thr Ala Arg Gly Gln Leu Phe Leu Arg Asn Leu Pro Ile Leu Ala

1400 1405 1410

Thr Lys Met Lys Leu Leu Met Val Gly Asn Leu Gly Ala Glu Ile

1415 1420 1425

Gly Asp Leu Glu His Leu Gly Trp Val Leu Arg Gly Pro Ala Val

1430 1435 1440

Cys Lys Lys Ile Thr Asn His Glu Lys Cys His Val Asn Ile Met

1445 1450 1455

Asp Lys Leu Thr Ala Phe Phe Gly Ile Met Pro Arg Gly Thr Thr

1460 1465 1470

Pro Arg Ala Pro Val Arg Phe Pro Thr Ala Leu Leu Lys Val Arg

1475 1480 1485

Arg Gly Leu Glu Thr Gly Trp Ala Tyr Thr His Gln Gly Gly Ile

1490 1495 1500

Ser Ser Val Asp His Val Thr Ala Gly Lys Asp Leu Leu Val Cys

1505 1510 1515

Asp Ser Met Gly Arg Thr Arg Val Val Cys His Ser Asn Asn Lys

1520 1525 1530

Met Thr Asp Glu Thr Glu Tyr Gly Ile Lys Thr Asp Ser Gly Cys

1535 1540 1545

Pro Glu Gly Ala Arg Cys Tyr Val Leu Asn Pro Glu Ala Val Asn

1550 1555 1560

Ile Ser Gly Thr Lys Gly Ala Met Val His Leu Gln Lys Thr Gly

1565 1570 1575

Gly Glu Phe Thr Cys Val Thr Ala Ser Gly Thr Pro Ala Phe Phe

1580 1585 1590

Asp Leu Lys Asn Leu Lys Gly Trp Ser Gly Leu Pro Ile Phe Glu

1595 1600 1605

Ala Ser Ser Gly Arg Val Val Gly Arg Val Lys Val Gly Lys Asn

1610 1615 1620

Glu Asp Ser Lys Pro Thr Lys Leu Met Ser Gly Ile Gln Thr Val

1625 1630 1635

Ser Lys Asn Gln Thr Asp Leu Ala Asp Ile Val Lys Lys Leu Thr

1640 1645 1650

Ser Met Asn Arg Gly Glu Phe Lys Gln Ile Thr Leu Ala Thr Gly

1655 1660 1665

Ala Gly Lys Thr Thr Glu Leu Pro Arg Ser Val Ile Glu Glu Ile

1670 1675 1680

Gly Arg His Lys Arg Val Leu Val Leu Ile Pro Leu Arg Ala Ala

1685 1690 1695

Ala Glu Ser Val Tyr Gln Tyr Met Arg Val Lys Tyr Pro Ser Ile

1700 1705 1710

Ser Phe Asn Leu Arg Ile Gly Asp Met Lys Glu Gly Asp Met Ala

1715 1720 1725

Thr Gly Ile Thr Tyr Ala Ser Tyr Gly Tyr Phe Cys Gln Leu Pro

1730 1735 1740

Gln Pro Lys Leu Arg Ala Ala Met Val Glu Tyr Ser Tyr Ile Phe

1745 1750 1755

Leu Asp Glu Tyr His Cys Ala Thr Pro Glu Gln Leu Ala Ile Ile

1760 1765 1770

Gly Lys Ile His Arg Phe Ala Glu Asn Leu Arg Val Val Ala Met

1775 1780 1785

Thr Ala Thr Pro Ala Gly Thr Val Thr Thr Thr Gly Gln Lys His

1790 1795 1800

Pro Ile Glu Glu Phe Ile Ala Pro Glu Val Met Lys Gly Glu Asp

1805 1810 1815

Leu Gly Ser Glu Tyr Leu Asp Ile Ala Gly Leu Lys Ile Pro Thr

1820 1825 1830

Glu Glu Met Lys Gly Asn Met Leu Val Phe Ala Pro Thr Arg Asn

1835 1840 1845

Met Ala Val Glu Thr Ala Lys Lys Leu Lys Ala Lys Gly Tyr Asn

1850 1855 1860

Ser Gly Tyr Tyr Tyr Ser Gly Glu Asn Pro Glu Asn Leu Arg Val

1865 1870 1875

Val Thr Ser Gln Ser Pro Tyr Val Val Val Ala Thr Asn Ala Ile

1880 1885 1890

Glu Ser Gly Val Thr Leu Pro Asp Leu Asp Thr Val Val Asp Thr

1895 1900 1905

Gly Leu Lys Cys Glu Lys Arg Val Arg Ile Ser Ser Lys Met Pro

1910 1915 1920

Phe Ile Val Thr Gly Leu Lys Arg Met Ala Val Thr Ile Gly Glu

1925 1930 1935

Gln Ala Gln Arg Arg Gly Arg Val Gly Arg Val Lys Pro Gly Arg

1940 1945 1950

Tyr Tyr Arg Ser Gln Glu Thr Ala Ser Gly Ser Lys Asp Tyr His

1955 1960 1965

Tyr Asp Leu Leu Gln Ala Gln Arg Tyr Gly Ile Glu Asp Gly Ile

1970 1975 1980

Asn Val Thr Lys Ser Phe Arg Glu Met Asn Tyr Asp Trp Ser Leu

1985 1990 1995

Tyr Glu Glu Asp Ser Leu Met Ile Thr Gln Leu Glu Val Leu Asn

2000 2005 2010

Asn Leu Leu Ile Ser Glu Asp Leu Pro Ala Ala Val Lys Asn Ile

2015 2020 2025

Met Ala Arg Thr Asp His Pro Glu Pro Ile Gln Leu Ala Tyr Asn

2030 2035 2040

Ser Tyr Glu Asn Gln Ile Pro Val Leu Phe Pro Lys Ile Lys Asn

2045 2050 2055

Gly Glu Val Thr Asp Ser Tyr Glu Asn Tyr Thr Tyr Leu Asn Ala

2060 2065 2070

Arg Lys Leu Gly Glu Asp Val Pro Ala Tyr Val Tyr Ala Thr Glu

2075 2080 2085

Asp Glu Asp Leu Ala Val Asp Leu Leu Gly Met Asp Trp Pro Asp

2090 2095 2100

Pro Gly Asn Gln Gln Val Val Glu Thr Gly Arg Ala Leu Lys Gln

2105 2110 2115

Val Thr Gly Leu Ser Thr Ala Glu Asn Ala Leu Leu Ile Ala Leu

2120 2125 2130

Phe Gly Tyr Val Gly Tyr Gln Thr Leu Ser Lys Arg His Ile Pro

2135 2140 2145

Met Ile Thr Asp Ile Tyr Thr Leu Glu Asp His Arg Leu Glu Asp

2150 2155 2160

Thr Thr His Leu Gln Phe Ala Pro Asn Ala Ile Arg Thr Asp Gly

2165 2170 2175

Lys Asp Ser Glu Leu Lys Glu Leu Ala Val Gly Asp Leu Asp Lys

2180 2185 2190

Tyr Val Asp Ala Leu Val Asp Tyr Ser Lys Gln Gly Met Lys Phe

2195 2200 2205

Ile Lys Val Gln Ala Glu Lys Val Arg Asp Ser Gln Ser Thr Lys

2210 2215 2220

Glu Gly Leu Gln Thr Ile Lys Glu Tyr Val Asp Lys Phe Ile Gln

2225 2230 2235

Ser Leu Thr Glu Asn Lys Glu Glu Ile Ile Arg Tyr Gly Leu Trp

2240 2245 2250

Gly Val His Thr Ala Leu Tyr Lys Ser Leu Ala Ala Arg Leu Gly

2255 2260 2265

His Glu Thr Ala Phe Ala Thr Leu Val Val Lys Trp Leu Ala Phe

2270 2275 2280

Gly Gly Glu Thr Val Ser Ala His Ile Lys Gln Val Ala Val Asp

2285 2290 2295

Leu Val Val Tyr Tyr Ile Ile Asn Lys Pro Ser Phe Pro Gly Asp

2300 2305 2310

Thr Glu Thr Gln Gln Glu Gly Arg Arg Phe Val Ala Ser Leu Phe

2315 2320 2325

Ile Ser Ala Leu Ala Thr Tyr Thr Tyr Lys Thr Trp Asn Tyr Asn

2330 2335 2340

Asn Leu Gln Arg Val Val Glu Pro Ala Leu Ala Tyr Leu Pro Tyr

2345 2350 2355

Ala Thr Ser Ala Leu Lys Leu Phe Thr Pro Thr Arg Leu Glu Ser

2360 2365 2370

Val Val Ile Leu Ser Ser Thr Ile Tyr Lys Thr Tyr Leu Ser Ile

2375 2380 2385

Arg Lys Gly Lys Ser Asp Gly Leu Leu Gly Thr Gly Ile Ser Ala

2390 2395 2400

Ala Met Glu Ile Leu Asn Gln Asn Pro Ile Ser Val Gly Ile Ser

2405 2410 2415

Val Met Leu Gly Val Gly Ala Ile Ala Ala His Asn Ala Ile Glu

2420 2425 2430

Ser Ser Glu Gln Lys Arg Thr Leu Leu Met Lys Val Phe Val Lys

2435 2440 2445

Asn Phe Leu Asp Gln Ala Ala Thr Asp Glu Leu Val Lys Glu Asn

2450 2455 2460

Pro Glu Lys Ile Ile Met Ala Leu Phe Glu Ala Val Gln Thr Ile

2465 2470 2475

Gly Asn Pro Leu Arg Leu Ile Tyr His Leu Tyr Gly Val Tyr Tyr

2480 2485 2490

Lys Gly Trp Glu Ala Lys Glu Leu Ala Glu Lys Thr Ala Gly Arg

2495 2500 2505

Asn Leu Phe Thr Leu Ile Met Phe Glu Ala Phe Glu Leu Leu Gly

2510 2515 2520

Met Asp Ser Glu Gly Lys Ile Arg Asn Leu Ser Gly Asn Tyr Ile

2525 2530 2535

Leu Asp Leu Ile Phe Asn Leu His Asn Lys Leu Asn Lys Gly Leu

2540 2545 2550

Lys Lys Leu Val Leu Gly Trp Ala Pro Ala Pro Leu Ser Cys Asp

2555 2560 2565

Trp Thr Pro Ser Asp Glu Arg Ile Ser Leu Pro His Asn Asn Tyr

2570 2575 2580

Leu Arg Val Glu Thr Arg Cys Pro Cys Gly Tyr Glu Met Lys Ala

2585 2590 2595

Ile Lys Asn Val Ala Gly Lys Leu Thr Lys Val Glu Glu Lys Gly

2600 2605 2610

Ser Phe Leu Cys Arg Asn Arg Leu Gly Arg Gly Pro Pro Asn Phe

2615 2620 2625

Lys Val Thr Lys Phe Tyr Asp Asp Asn Leu Ile Glu Val Lys Pro

2630 2635 2640

Val Ala Arg Leu Glu Gly Gln Val Asp Leu Tyr Tyr Lys Gly Val

2645 2650 2655

Thr Ala Lys Leu Asp Tyr Asn Asn Gly Lys Val Leu Leu Ala Thr

2660 2665 2670

Asn Lys Trp Glu Val Asp His Ala Phe Leu Thr Arg Leu Val Lys

2675 2680 2685

Lys His Thr Gly Ile Gly Phe Lys Gly Ala Tyr Leu Gly Asp Arg

2690 2695 2700

Pro Asp His Gln Asp Leu Val Asp Arg Asp Cys Ala Thr Ile Thr

2705 2710 2715

Lys Asn Ser Val Gln Phe Leu Lys Met Lys Lys Gly Cys Ala Phe

2720 2725 2730

Thr Tyr Asp Leu Thr Ile Ser Asn Leu Val Arg Leu Ile Glu Leu

2735 2740 2745

Val His Lys Asn Asn Leu Gln Glu Arg Glu Ile Pro Thr Val Thr

2750 2755 2760

Val Thr Thr Trp Leu Ala Tyr Ser Phe Val Asn Glu Asp Leu Gly

2765 2770 2775

Thr Ile Lys Pro Val Leu Gly Glu Lys Val Ile Pro Glu Pro Pro

2780 2785 2790

Glu Glu Leu Ser Leu Gln Pro Thr Val Arg Leu Val Thr Thr Glu

2795 2800 2805

Thr Ala Ile Thr Ile Thr Gly Glu Ala Glu Val Met Thr Thr Gly

2810 2815 2820

Ile Thr Pro Val Val Glu Met Lys Glu Glu Pro Gln Leu Asp His

2825 2830 2835

Gln Ser Thr Thr Leu Lys Val Gly Leu Lys Glu Gly Glu Tyr Pro

2840 2845 2850

Gly Pro Gly Val Asn Pro Asn His Leu Ala Glu Val Ile Asp Glu

2855 2860 2865

Lys Asp Asp Arg Pro Phe Val Leu Ile Ile Gly Asn Lys Gly Ser

2870 2875 2880

Thr Ser Asn Arg Ala Arg Thr Ala Lys Asn Ile Arg Leu Tyr Lys

2885 2890 2895

Gly Asn Asn Pro Arg Glu Ile Arg Asp Leu Met Ser Gln Gly Arg

2900 2905 2910

Ile Leu Thr Val Ala Leu Lys Glu Leu Asp Pro Glu Leu Lys Glu

2915 2920 2925

Leu Val Asp Tyr Lys Gly Thr Phe Leu Asn Arg Glu Ala Leu Glu

2930 2935 2940

Ala Leu Ser Leu Gly Lys Pro Ile Lys Arg Lys Thr Thr Thr Ala

2945 2950 2955

Met Ile Arg Arg Leu Ile Glu Pro Glu Val Glu Glu Glu Leu Pro

2960 2965 2970

Asp Trp Phe Gln Ala Glu Glu Pro Leu Phe Leu Glu Ala Lys Ile

2975 2980 2985

Gln Asn Asp Leu Tyr His Leu Ile Gly Ser Val Asp Ser Ile Lys

2990 2995 3000

Ser Lys Ala Lys Glu Leu Gly Ala Thr Asp Asn Thr Lys Ile Val

3005 3010 3015

Lys Glu Val Gly Ala Arg Thr Tyr Thr Met Lys Leu Ser Ser Trp

3020 3025 3030

Ser Thr Gln Val Thr Lys Lys Gln Met Ser Leu Ala Pro Leu Phe

3035 3040 3045

Glu Glu Leu Leu Leu Lys Cys Pro Pro Cys Ser Lys Ile Ser Lys

3050 3055 3060

Gly His Met Val Ser Ala Tyr Gln Leu Ala Gln Gly Asn Trp Glu

3065 3070 3075

Pro Leu Gly Cys Gly Val Tyr Met Gly Thr Ile Pro Ala Arg Arg

3080 3085 3090

Leu Lys Ile His Pro Tyr Glu Ala Tyr Leu Lys Leu Lys Glu Leu

3095 3100 3105

Val Glu Val Glu Ser Ser Arg Ala Thr Ala Lys Glu Ser Ile Ile

3110 3115 3120

Arg Glu His Asn Thr Trp Ile Leu Arg Lys Val Arg His Glu Gly

3125 3130 3135

Asn Leu Arg Thr Lys Ser Met Ile Asn Pro Gly Lys Ile Ser Asp

3140 3145 3150

Gln Leu Cys Arg Asp Gly His Lys Arg Asn Ile Tyr Asn Lys Ile

3155 3160 3165

Ile Gly Ser Thr Met Ala Ser Ala Gly Ile Arg Leu Glu Lys Leu

3170 3175 3180

Pro Val Val Arg Ala Gln Thr Asp Thr Thr Ser Phe His Gln Ala

3185 3190 3195

Ile Arg Glu Lys Ile Asp Lys Thr Glu Asn Lys Gln Thr Pro Glu

3200 3205 3210

Leu His Glu Glu Leu Met Lys Val Phe Asp Cys Leu Lys Ile Pro

3215 3220 3225

Glu Leu Lys Glu Ser Tyr Asp Glu Val Ser Trp Glu Gln Leu Glu

3230 3235 3240

Ala Gly Ile Asn Arg Lys Gly Ala Ala Gly Tyr Leu Glu Ser Lys

3245 3250 3255

Asn Ile Gly Glu Val Leu Asp Thr Glu Lys His Ile Val Glu Gln

3260 3265 3270

Leu Ile Lys Asp Leu Arg Lys Gly Lys Lys Ile Arg Tyr Tyr Glu

3275 3280 3285

Thr Ala Ile Pro Lys Asn Glu Lys Arg Asp Val Ser Asp Asp Trp

3290 3295 3300

Glu Ala Gly Glu Phe Val Asp Glu Lys Lys Pro Arg Val Ile Gln

3305 3310 3315

Tyr Pro Asp Ala Lys Val Arg Leu Ala Ile Thr Lys Val Met Tyr

3320 3325 3330

Lys Trp Val Lys Gln Lys Pro Val Val Ile Pro Gly Tyr Glu Gly

3335 3340 3345

Lys Thr Pro Leu Phe Asp Ile Phe Asn Lys Val Lys Lys Glu Trp

3350 3355 3360

Asp Ser Phe Gln Asp Pro Val Ala Val Ser Phe Asp Thr Lys Ala

3365 3370 3375

Trp Asp Thr Gln Val Thr Ser Arg Asp Leu Met Leu Ile Lys Asp

3380 3385 3390

Ile Gln Lys Tyr Tyr Phe Lys Arg Ser Ile His Lys Phe Leu Asp

3395 3400 3405

Thr Ile Thr Glu His Met Val Glu Val Pro Val Ile Thr Ala Asp

3410 3415 3420

Gly Glu Val Tyr Ile Arg Asn Gly Gln Arg Gly Ser Gly Gln Pro

3425 3430 3435

Aso Thr Ser Ala Gly Asn Ser Met Leu Asn Val Leu Thr Met Ile

3440 3445 3450

Tyr Ala Phe Cys Lys Ser Thr Gly Ile Pro Tyr Arg Gly Phe Ser

3455 3460 3465

Arg Val Ala Arg Ile His Val Cys Gly Asp Asp Gly Phe Leu Ile

3470 3475 3480

Thr Glu Arg Gly Leu Gly Leu Lys Phe Ser Glu Lys Gly Met Gln

3485 3490 3495

Ile Leu His Glu Ala Gly Lys Pro Gln Lys Ile Thr Glu Gly Asp

3500 3505 3510

Lys Met Lys Val Ala Tyr Arg Phe Glu Asp Ile Glu Phe Cys Ser

3515 3520 3525

His Thr Pro Val Pro Val Arg Trp Ala Asp Asn Thr Ser Ser Tyr

3530 3535 3540

Met Ala Gly Arg Ser Thr Ala Thr Ile Leu Ala Lys Met Ala Thr

3545 3550 3555

Arg Leu Asp Ser Ser Gly Glu Arg Gly Ser Thr Ala Tyr Glu Lys

3560 3565 3570

Ala Val Ala Phe Ser Phe Leu Leu Met Tyr Ser Trp Asn Pro Val

3575 3580 3585

Val Arg Arg Ile Cys Leu Leu Val Leu Ser Gln Phe Pro Glu Ile

3590 3595 3600

Ser Pro Ser Lys Asn Thr Ile Tyr Tyr Tyr Gln Gly Asp Pro Ile

3605 3610 3615

Ala Ala Tyr Arg Glu Val Ile Gly Lys Gln Leu Cys Glu Leu Lys

3620 3625 3630

Arg Thr Gly Phe Glu Lys Leu Ala Gly Leu Asn Leu Ser Met Thr

3635 3640 3645

Thr Leu Gly Ile Trp Thr Lys His Thr Ser Lys Arg Leu Ile Gln

3650 3655 3660

Ala Cys Val Glu Ile Gly Lys Arg Glu Gly Thr Trp Leu Val Asn

3665 3670 3675

Ala Asp Arg Leu Ile Ala Gly Lys Thr Gly Lys Phe Tyr Ile Pro

3680 3685 3690

Ser Thr Gly Val Thr Leu Leu Gly Lys His Tyr Glu Glu Ile Asn

3695 3700 3705

Leu Lys Gln Lys Ala Ala Gln Pro Pro Ile Glu Gly Val Asp Arg

3710 3715 3720

Tyr Lys Leu Gly Pro Ile Val Asn Val Ile Leu Arg Arg Leu Arg

3725 3730 3735

Val Met Leu Met Thr Val Ala Ser Gly Ser Trp

3740 3745

Claims

1.一种减毒的牛病毒性腹泻病毒，其在糖蛋白E^rns编码序列中有至少一个突变且在N^pro编码序列中另有至少一个突变，其中

a.在糖蛋白E^rns编码序列中的所述突变位于编码位点298至310的氨基酸和/或位点341至360的氨基酸的核苷酸序列中且导致存在于E^rns中的RNA酶活性失活，所述编号参照牛病毒性腹泻病毒cp7株已公开的氨基酸序列，和

b.在N^pro编码序列中的所述突变导致该N^pro的蛋白酶活性失活。

2.权利要求1的病毒，其中所述突变是删除。

3.权利要求1或2的病毒，其中在糖蛋白E^rns编码序列中的所述突变位于编码E^rns保守序列SLHGIWPEKICTG和/或LQRHEWNKHGWCN WFHIEPW的核苷酸序列中。

4.权利要求1至3之一的病毒，其中在糖蛋白E^rns编码序列中的所述突变位于编码E^rns保守序列SLHGIWPEKIC和/或RHEWNKHGWCNW的核苷酸序列中。

5.权利要求1至4之一的病毒，其中在糖蛋白E^rns编码序列中的所述突变是位于E^rns保守序列SLHGIWPEKIC或RHEWNKHGWCNW中的单突变。

6.权利要求1-5之一的病毒，其中所述突变选自：

i)S298G，

ii)W303G，

iii)H349L或H349K，

iv)H300K加H349K，或者H300K加H349L，

v)H300L加H349L，

vi)删除组氨酸349(349del)，

所述编号参照牛病毒性腹泻病毒cp7株已公开的氨基酸序列。

7.权利要求1至6之一的病毒，其中在N^pro编码序列中的所述突变导致编码的聚蛋白有下列通式的特征：

[N^pro]_x-[PS]_y-[C-term]

并且其中：

[N^pro]是指该聚蛋白的N^pro部分，其中“x”代表该聚蛋白中N^pro的氨基酸数目；且其中

[PS]是指选自下列的加工信号：泛素和LC3；并且其中

“Y”＝0，其意味着不存在加工信号，或“Y”＝1，其意味着存在加工信号；并且其中

[C-term]是指完整的病毒聚蛋白，其除外N^pro，但是包括衣壳(C)-蛋白和该病毒聚蛋白中存在的包括羧基端NS5B在内的任何其它蛋白；并且其中

当“y”＝0时，“x”是0至12，这意味着无N^pro特异性氨基酸或仅存在N^pro的1至12个氨基酸；并且其中

当“y”＝1时，“x”是0至168；这意味着无N^pro特异性氨基酸或存在N^pro的1至全部168个氨基酸。

8.权利要求7的病毒，其中在N^pro编码序列中的所述突变导致编码的聚蛋白有下列通式的特征：

[N^pro]₁-[PS]₀-[C-term]，

并且其中的含义按照权利要求7定义。

9.权利要求7的病毒，其中在N^pro编码序列中的所述突变导致编码的聚蛋白有下列通式的特征：

[N^pro]₃-[PS]₀-[C-term]，

并且其中的含义按照权利要求7定义。

10.权利要求7的病毒，其中在N^pro编码序列中的所述突变导致编码的聚蛋白有下列通式的特征：

[N^pro]₄-[PS]₀-[C-term]，

并且其中的含义按照权利要求7定义。

11.权利要求7的病毒，其中在N^pro编码序列中的所述突变导致编码的聚蛋白有下列通式的特征：

[N^pro]₆-[PS]₀-[C-term]，

并且其中的含义按照权利要求7定义。

12.权利要求7的病毒，其中在N^pro编码序列中的所述突变导致编码的聚蛋白有下列通式的特征：

[N^pro]₄-[PS]₀-[C-term]*，

并且其中[C-term]*是指其C-蛋白中位点2的氨基酸从D变成N后得到的[C-term]。

13.权利要求7的病毒，其中在N^pro编码序列中的突变导致编码的聚蛋白有选自下列通式的特征：

M-[PS]₀-[C-term]；

MEL-[PS]₀-[C-term]；

MELF-[PS]₀-[C-term]；

MELFS-[PS]₀-[C-term]；

MELFSN-[PS]₀-[C-term]；

MELFSNE-[PS]₀-[C-term]；

MELFSNEL-[PS]₀-[C-term]；

MELFSNELL-[PS]₀-[C-term]；

MELFSNELLY-[PS]₀-[C-term]；

MELFSNELLYK-[PS]₀-[C-term]；

MELFSNELLYKT-[PS]₀-[C-term]。

14.权利要求7的病毒，其中在N^pro编码序列中的所述突变导致编码的聚蛋白有选自下列通式的特征：

MELI-[PS]₀-[C-term]；

MELIS-[PS]₀-[C-term]；

MELISN-[PS]₀-[C-term]；

MELISNE-[PS]₀-[C-term]；

MELISNEL-[PS]₀-[C-term]；

MELISNELL-[PS]₀-[C-term]；

MELISNELLY-[PS]₀-[C-term]；

MELISNELLYK-[PS]₀-[C-term]；

MELISNELLYKT-[PS]₀-[C-term]。

15.权利要求7的病毒，其中在N^pro编码序列中的突变导致编码的聚蛋白有选自下列通式的特征：

MELIT-[PS]₀-[C-term]；

MELITN-[PS]₀-[C-term]；

MELITNE-[PS]₀-[C-term]；

MELITNEL-[PS]₀-[C-term]；

MELITNELL-[PS]₀-[C-term]；

MELITNELLY-[PS]₀-[C-term]；

MELITNELLYK-[PS]₀-[C-term]；

MELITNELLYKT-[PS]₀-[C-term]。

16.权利要求1至15之一的病毒，其中该减毒牛病毒性腹泻病毒为减毒牛病毒性腹泻病毒1型。

17.权利要求1至15之一的病毒，其中该减毒牛病毒性腹泻病毒为减毒牛病毒性腹泻病毒2型。

18.权利要求1的病毒，其中该减毒牛病毒性腹泻病毒的编码区由氨基酸序列SEQ ID No.8编码。

19.权利要求18的病毒，其中该减毒牛病毒性腹泻病毒为减毒牛病毒性腹泻病毒2型。

20.组合物，包括溶液和权利要求1至19之一的减毒牛病毒性腹泻病毒。

21.权利要求20的组合物，其在动物中诱导免疫应答。

22.权利要求20或21的组合物，其是疫苗。

23.权利要求20至22之一的组合物，其中该组合物进一步包括可药用的载体或赋形剂。

24.权利要求1至19之一的病毒在制备用于预防和治疗牛病毒性腹泻病毒感染的疫苗中的用途。

25.包括编码权利要求1至19之一所述病毒的核酸的核酸分子，或其基于简并核酸密码的变体。

26.权利要求25的核酸分子，其中该核酸分子是DNA。

27.权利要求25的核酸分子，其中该核酸分子是RNA。

28.使牛病毒性腹泻病毒减毒的方法，特征在于，在牛病毒性腹泻病毒中产生突变，即糖蛋白E^rns编码序列中的至少一个突变和N^pro编码序列中的至少另一个突变，其中：

a.在糖蛋白E^rns编码序列中的所述突变位于编码位点298至310的氨基酸和/或位点341至360的氨基酸的核苷酸序列中且导致存在于E^rns中的 RNA酶活性失活，所述编号参照牛病毒性腹泻病毒cp7株已公开的氨基酸序列，和

29.权利要求28的方法，包括下列步骤：

a)将野生型牛病毒性腹泻病毒核苷酸序列逆转录成cDNA；

b)克隆该cDNA；

c)向该cDNA中导入位于糖蛋白E^rns和N^pro的编码序列中的突变，

d)将该cDNA掺入能在体外或感染合适的细胞后指导将牛病毒性腹泻病毒cDNA转录成RNA的质粒或DNA病毒中。

30.权利要求20的组合物，其中所述组合物包含权利要求16所述的减毒牛病毒性腹泻病毒1型以及权利要求17或19所述的减毒牛病毒性腹泻病毒2型。

31.权利要求16所述的减毒牛病毒性腹泻病毒1型以及权利要求17或19所述的减毒牛病毒性腹泻病毒2型组合用于制备预防和治疗牛病毒性腹泻病毒感染的疫苗的用途。

32.权利要求16所述的减毒牛病毒性腹泻病毒1型病毒在制备组合物中的用途，所述组合物是在育种牛群、怀孕母牛中预防和/或治疗牛病毒性腹泻病毒1型感染的组合物，或者是在怀孕母牛中诱导对抗牛病毒性腹泻病毒1型感染的胎儿保护作用的组合物。

33.权利要求16所述的减毒牛病毒性腹泻病毒1型病毒在制备组合物中的用途，所述组合物是在育种牛群、怀孕母牛中预防和/或治疗牛病毒性腹泻病毒2型感染的组合物，或者是在怀孕母牛中诱导对抗牛病毒性腹泻病毒2型感染的胎儿保护作用的组合物。

34.权利要求17或19所述的减毒牛病毒性腹泻病毒2型病毒在制备组合物中的用途，所述组合物是在育种牛群、怀孕母牛中预防和/或治疗牛病毒性腹泻病毒1型感染的组合物，或者是在怀孕母牛中诱导对抗牛病毒性腹泻病毒1型感染的胎儿保护作用的组合物。

35.权利要求16所述的减毒牛病毒性腹泻病毒1型病毒与权利要求17或19所述的减毒牛病毒性腹泻病毒2型病毒组合用于制备组合物的用途，所述组合物是在育种牛群、怀孕母牛中预防和/或治疗牛病毒性腹泻病毒1 型和/或2型感染的组合物，或者是在怀孕母牛中诱导对抗牛病毒性腹泻病毒1型和2型感染的胎儿保护作用的组合物。