CN1961893A - Method for preparing extract of adult bombay canary - Google Patents
Method for preparing extract of adult bombay canary Download PDFInfo
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- CN1961893A CN1961893A CNA200510032348XA CN200510032348A CN1961893A CN 1961893 A CN1961893 A CN 1961893A CN A200510032348X A CNA200510032348X A CN A200510032348XA CN 200510032348 A CN200510032348 A CN 200510032348A CN 1961893 A CN1961893 A CN 1961893A
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Abstract
The invention relates to a method for preparing America roach adult extractive, wherein it comprises that combines alcohol extraction and water extraction techniques, while the total recycle rate can reach 25.2% and the amino acid content of recycled product can reach 58.5%. The inventive method is simple with low cost and short time. The test on rat has proved that the invention has no side effect and toxic.
Description
Technical field
The present invention is specifically related to periplaneta americana (Periplaneta americana) adult preparation method of extract.
Background technology
Cockroach (Periplaneta) is a kind of ancient insecticide (Blatta seu periplaneta), survived on earth 3.5 hundred million years, be considered as insect and eliminate it by the mankind always over the past thousands of years, yet the means of killing in the face of the mankind are the most original or go-go still can indomitablely be survived so far, as seen have classic bioactive substance and special physiological mechanism in its body, and superpower life-information maintenance system, thereby it is the most ancient in the world just it to be become, and multiplies the most successful so far insecticide monoid.Functions such as " Dictionary of Chinese Pharmacy ", " Chinese medicine voluminous dictionary ", " Chinese medicinal animal will ", " national Chinese herbal medicine compilation " etc. are near, the modern pharmacy monograph is all put down in writing cockroach has " invigorate blood circulation, dissipating blood stasis, eliminate indigestion, the infantile malnutrition that disappears, detoxifcation, diuresis, detumescence ", and be used for the treatment of furuncle, throat moth, carbuncle, snake bite and insect sting etc.Scholars such as Yao Lian think through textual criticism, the medicinal cockroach of putting down in writing in the above ancient books and records, its Ji Yuan and former animal should be periplaneta americana (Periplanetaamericana), Australian cockroach (P.australasiae), periplaneta brunnea (P.brunnea), Peroplaneta fluligginosa (P.fuliginosa) of Blattaria Blattidae Periplaneta etc.
The cockroach extractive of tool active component can be used for treating outer wound face, female genital diseases, cardiovascular disease and hepatitis B etc.Chinese patent CN87100537 discloses " a kind of preparation method for the treatment of outer wound face medicine ", this method is: with coarse pulverization after the cockroach polypide drying, with alcohol solution dipping to extract wherein effective medicinal components, separate filtering residue and filtrate then, and filtering residue extracted repeatedly, concentrate gained filtrate and obtain thick thing, the treated outer wound face medicine of a kind of treatment of making.Chinese patent CN1135895A discloses " a kind of medicine for the treatment of female genital diseases and uses thereof ", the cockroach ethanol extraction adds adjuvant and can be made into suppository, effervescent tablet, tablet etc., aspect diseases such as treatment cervical erosion, vaginitis, cervical cancer, have short treating period, good effect, advantage that toxic and side effects is few.Chinese patent 94118839 discloses in the Blatta seu periplaneta body and extracted active component, removes degrease behind the employing ethanol extraction, makes water solublity extractum, and is evident in efficacy to cardiovascular disease with the medicine that compound nucleoside compatibility is made.Chinese patent 94108943 discloses with the contract mixture of amino acids of the intravital mucopolysaccharide of ethanol extraction, defat, activated carbon adsorption, branch molten acquisition Blatta seu periplaneta, is mainly used in the treatment of hepatitis B.To the research of Blatta seu periplaneta, also utilize corporacardiace treatment heart disease etc. abroad.
Bibliographical information is arranged, and the periplaneta americana body contains the active component of multiple tool medical value, and its lymph contains trehalose (trehalose), inositol (inositol), protocatechuic acid glucoside glycoproteins such as (protocatechuic acid glucoside); Contain several amino acids in cuticle and the muscle, glycosides propylhomoserin, alanine, leucine, isoleucine, phenylalanine, cystine, glutamic acid, histidine, serine, threonine, arginine, N etc. are wherein arranged.Contain multiple pheromone, neurotransmitter peptide and neural chromone C, D in addition, isoxanthopterin, 2-amino-4,6-dihydroxypteridine, 5-hydroxyl color ammonia, DOPA ammonia, r-aminobutyric acid, chrondroitin (chondroitin), dermatan (dermantan), keratan sulfate, hyaluronic acid (hyaluromic) etc.
The big cockroach extractive preparation method in existing America traditional ethanol extraction method or the simple water extraction methods of adopting cause a large amount of aminoacid to fail effectively to extract thus, and overall recovery is less than 10%, the aminoacid total content of product only 30%.
Summary of the invention
The object of the present invention is to provide a kind of preparation method of extract of adult bombay canary.This method can be extracted the various active component of periplaneta americana fast and effectively, as the natural extract of animal, and adult extract safe without toxic side effect of the present invention.
The present invention realizes according to following technical scheme:
The A water extraction:
1, slightly carries: choose adult bombay canary in January, be transferred to interior the raising 5-6 days of glass jar of supplying water but removing food,-20 ℃ of freezing 30min put to death after treating into the most content of vomiting caused by intestinal parasitosis, and 75% ethanol short time was cleaned polypide surface, twice of distilled water drip washing, airing, cut off and take by weighing about 10g behind wing, foot, the antenna and put into and grind alms bowl, add the 10ml distilled water 30min that mills, put-20 ℃ of refrigerator and cooled and freeze 15min, 3 times so repeatedly, fully worn into homogenate up to polypide.Homogenate is moved in the 50ml centrifuge tube, be settled to 40ml, the centrifugal 15min of 4000rpm, separate upper strata grease and lower sediment, supernatant in the middle of getting, the centrifugal 15min of 10000rpm, separate upper strata grease and lower sediment, supernatant in the middle of getting ,-4 ℃ of refrigerator standing over night.
2, concentrate, alcohol washes: be heated to explosive evaporation in boiling water bath, generate a large amount of taupes precipitations when treating supernatant concentration to original volume 1/4, the centrifugal 15min of cooling back 10000rpm discards upper strata grease and lower sediment, supernatant in the middle of getting.Be heated to about 60 ℃, add 95% ethanol (2: 1) while hot, constantly stir, be cooled to room temperature after, 10000rpm is centrifugal, and 15min removes supernatant, white depositions, ethanol repeated precipitation three times.
3, refining: extracting waste precipitate heating for dissolving, add 1/10-1/8 volume activity carbon again, constantly stir, 80 ℃ of heating 30min, filtered while hot with a small amount of distillation washing activated carbon secondary, merges each washing filtrate, 95% ethyl alcohol recrystallization.
B: alcohol extraction:
Water intaking is extracted the first step and is separated upper strata grease and the lower sediment that obtains, 85% ethanol that adds 2 times of volumes, hot reflux extracting 3-5 hour, the overall process temperature of alcohol extraction is controlled at about 75 ℃, extract heated in boiling water bath be concentrated into original volume 1/4, the centrifugal 15min of cooling back 10000rpm discards upper strata grease and lower sediment, adds activated carbon adsorption and makes with extra care.
Water extract mixed with the alcohol extract equal-volume promptly get extract of adult bombay canary of the present invention.
Preparation method of the present invention combines alcohol extraction with water extraction process, overall recovery reaches 25.2%, follows the example of yield than simple alcohol extraction and exceeds more than one times, reclaims the product amino acid content up to 58.5%.Described extraction preparation method step is simple, and solvent for use is cheap and easy to get, and extraction effect is good and extract complete.Acute toxicity test in mice show extract that the present invention makes safely, have no side effect.
The specific embodiment
Embodiment one: the extract of adult bombay canary preparation method
The A water extraction:
1, slightly carries: choose adult bombay canary in January, be transferred to interior the raising 5-6 days of glass jar of supplying water but removing food,-20 ℃ of freezing 30min put to death after treating into the most content of vomiting caused by intestinal parasitosis, and 75% ethanol short time was cleaned polypide surface, twice of distilled water drip washing, airing, cut off and take by weighing about 10g behind wing, foot, the antenna and put into and grind alms bowl, add the 10ml distilled water 30min that mills, put-20 ℃ of refrigerator and cooled and freeze 15min, 3 times so repeatedly, fully worn into homogenate up to polypide.Homogenate is moved in the 50ml centrifuge tube, be settled to 40ml, the centrifugal 15min of 4000rpm, separate upper strata grease and lower sediment, supernatant in the middle of getting, the centrifugal 15min of 10000rpm, separate upper strata grease and lower sediment, supernatant in the middle of getting ,-4 ℃ of refrigerator standing over night.
2, concentrate, alcohol washes: be heated to explosive evaporation in boiling water bath, generate a large amount of taupes precipitations when treating supernatant concentration to original volume 1/4, the centrifugal 15min of cooling back 10000rpm discards upper strata grease and lower sediment, clear liquor in the middle of getting.Be heated to about 60 ℃, add 95% ethanol (2: 1) while hot, constantly stir, be cooled to room temperature after, 10000rpm is centrifugal, and 15min removes supernatant, white depositions, ethanol repeated precipitation three times.
3, refining: extracting waste precipitate heating for dissolving, add 1/10-1/8 volume activity carbon again, constantly stir, 80 ℃ of heating 30min, filtered while hot with a small amount of distillation washing activated carbon secondary, merges each washing filtrate, 95% ethyl alcohol recrystallization.
B: alcohol extraction:
Water intaking is extracted the first step and is separated upper strata grease and the lower sediment that obtains, 85% ethanol that adds 2 times of volumes, hot reflux extracting 3-5 hour, the overall process temperature of alcohol extraction is controlled at about 75 ℃, extract heated in boiling water bath be concentrated into original volume 1/4, the centrifugal 15min of cooling back 10000rpm discards precipitation, adds the adsorption refining of 1/10-1/8 volume activity charcoal.
Water extract mixed with the alcohol extract equal-volume promptly get extract of adult bombay canary of the present invention.
Embodiment two: the American-cockroach-extract acute toxicity test in mice
40 of Kunming mouses, male and female half and half, body weight 20 ± 2g is divided into 4 groups at random, 10 every group.Water is can't help in fasting overnight before the animals administer, after the numbering with female worm extract and nymph extract through disposable gastric infusion.Two kinds are tried thing each minute four dosage groups, the maximal dose group is got stock solution, other dosage groups are by establishing spacing between group at 1: 0.8, irritating the stomach amount is the 0.25ml/10g body weight, at least at interval feed in 2 hours after the administration, 14 days toxic reaction behind the observation animal filling stomach is obtained and is tried the median lethal dose(LD 50) (LD of thing to mice continuously
50).
Mice is behind gastric infusion, observe and do not see dead mouse in 14 days continuously, and well-grown, and outward appearance, behavioral activity, the mental status, diet, defecation and color, skin and fur etc. there is no unusually, and body weight all has increase.Dissect all no abnormal variations of each internal organs such as the perusal heart, liver, spleen, lung, kidney after putting to death animal.Tried thing LD
50Greater than 1260mg/kg, show that being tried thing does not have the danger that serious acute is poisoned, and belongs to nontoxic substantially chemical compound test result and sees Table one:
Table one American-cockroach-extract acute toxic test result
| The animal group | Dosage (mg/kg) | Number of animals (only) | Dead animal number (only) | Mortality rate (%) |
| 1 2 3 4 | 1260 1000 800 640 | 10 10 10 10 | 0 0 0 0 | 0 0 0 0 |
The preparation technology of extract of adult bombay canary of the present invention is simple, is convenient to promote the use of; The extract that makes has comprised pure dissolubility and water miscible active component, extract fully, the acute toxicity test in mice result shows that extract has no side effect and is easily absorbed by the body, for pharmacy provides safety, fine raw material, can make pointed medicine according to clinical needs, have bigger application prospect.
Claims (1)
1, a kind of preparation method of extract of adult bombay canary is characterized in that being undertaken by following processing step:
The A water extraction:
(1) slightly carries: choose adult bombay canary in January, be transferred to interior the raising 5-6 days of glass jar of supplying water but removing food,-20 ℃ of freezing 30min put to death after treating into the most content of vomiting caused by intestinal parasitosis, 75% ethanol Rapid Cleaning polypide surface, distilled water drip washing twice, airing, cut off and take by weighing 10g behind wing, foot, the antenna and put into mortar, add 10ml distilled water grinding 30min postposition-20 a ℃ refrigerator and cooled and freeze 15min, 3 times so repeatedly, fully worn into homogenate up to polypide.Homogenate is moved in the 50ml centrifuge tube, be settled to 40ml, the centrifugal 15min of 4000rpm, separate upper strata grease and lower sediment, supernatant in the middle of getting, the centrifugal 15min of 10000rpm, separate upper strata grease and lower sediment, supernatant in the middle of getting ,-4 ℃ of refrigerator standing over night.
(2) concentrate, alcohol washes: be heated to explosive evaporation in boiling water bath, generate a large amount of taupes precipitations when treating supernatant concentration to original volume 1/4, the centrifugal 15min of cooling back 10000rpm discards upper strata grease and lower sediment, supernatant in the middle of getting.Be heated to about 60 ℃, add 95% ethanol (2: 1) while hot, constantly stir, be cooled to room temperature after, 10000rpm is centrifugal, and 15min removes supernatant, white depositions, ethanol repeated precipitation three times.
(3) refining: extracting waste precipitate heating for dissolving, constantly stir behind the adding 1/10-1/8 volume activity carbon, 80 ℃ of heating 30min, filtered while hot with a small amount of washing activated carbon secondary that distills, merges each washing filtrate, 95% ethyl alcohol recrystallization.
B: alcohol extraction:
Water intaking is extracted the first step and is separated upper strata grease and the lower sediment that obtains, 85% ethanol that adds 2 times of volumes, hot reflux extracting 1-2 hour, the overall process temperature of alcohol extraction is controlled at about 75 ℃, extract heated in boiling water bath be concentrated into original volume 1/4, the centrifugal 15min of cooling back 10000rpm discards upper strata grease and lower sediment, adds activated carbon adsorption and makes with extra care.
Water extract mixed with the alcohol extract equal-volume promptly get extract of adult bombay canary of the present invention.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA200510032348XA CN1961893A (en) | 2005-11-08 | 2005-11-08 | Method for preparing extract of adult bombay canary |
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|---|---|---|---|
| CNA200510032348XA CN1961893A (en) | 2005-11-08 | 2005-11-08 | Method for preparing extract of adult bombay canary |
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| CN1961893A true CN1961893A (en) | 2007-05-16 |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102349931A (en) * | 2011-08-12 | 2012-02-15 | 云南省腾冲制药厂 | Cockroach extract, preparation method thereof, and application of cockroach extract preparation in treatment of cardiovascular disease |
| CN101502534B (en) * | 2009-03-11 | 2012-05-23 | 黄少鉴 | Method for extracting medicinal material from Periplaneta americana |
| CN102846667A (en) * | 2012-08-23 | 2013-01-02 | 耿福能 | Preparation method for nanometer Periplaneta americana through low temperature drying |
| CN102108090B (en) * | 2009-12-23 | 2013-04-03 | 浙江京新药业股份有限公司 | Dihydroiso-coumarin glucoside compounds and preparation method and application thereof |
| CN103690457A (en) * | 2012-08-31 | 2014-04-02 | 耿福能 | Toothpaste and preparation method thereof |
| CN104337842A (en) * | 2013-08-02 | 2015-02-11 | 成都百草和济科技有限公司 | Periplaneta americana medicine material and preparation method thereof |
| CN105434310A (en) * | 2015-12-22 | 2016-03-30 | 广州市娇兰化妆品有限公司 | Application of periplaneta americana active ingredient extract in cosmetic preparation |
| CN111925408A (en) * | 2020-08-14 | 2020-11-13 | 云南中医药大学 | Preparation method of periplaneta americana polypeptide extract by using water as medium |
| JP2022551319A (en) * | 2019-10-11 | 2022-12-08 | リャンシャン ジァネンダ バイオマテリアル ブリーディングシーオー．，エルティーディー． | American cockroach extract, formulation, preparation method and use thereof |
-
2005
- 2005-11-08 CN CNA200510032348XA patent/CN1961893A/en active Pending
Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101502534B (en) * | 2009-03-11 | 2012-05-23 | 黄少鉴 | Method for extracting medicinal material from Periplaneta americana |
| CN102108090B (en) * | 2009-12-23 | 2013-04-03 | 浙江京新药业股份有限公司 | Dihydroiso-coumarin glucoside compounds and preparation method and application thereof |
| CN102349931A (en) * | 2011-08-12 | 2012-02-15 | 云南省腾冲制药厂 | Cockroach extract, preparation method thereof, and application of cockroach extract preparation in treatment of cardiovascular disease |
| CN102846667A (en) * | 2012-08-23 | 2013-01-02 | 耿福能 | Preparation method for nanometer Periplaneta americana through low temperature drying |
| CN102846667B (en) * | 2012-08-23 | 2013-06-12 | 耿福能 | Preparation method for nanometer Periplaneta americana through low temperature drying |
| CN103690457B (en) * | 2012-08-31 | 2016-10-26 | 耿福能 | A kind of toothpaste and preparation method thereof |
| CN103690457A (en) * | 2012-08-31 | 2014-04-02 | 耿福能 | Toothpaste and preparation method thereof |
| CN104337842A (en) * | 2013-08-02 | 2015-02-11 | 成都百草和济科技有限公司 | Periplaneta americana medicine material and preparation method thereof |
| CN104337842B (en) * | 2013-08-02 | 2018-08-14 | 成都百草和济科技有限公司 | A kind of American cockroach medicinal material and preparation method thereof |
| CN105434310A (en) * | 2015-12-22 | 2016-03-30 | 广州市娇兰化妆品有限公司 | Application of periplaneta americana active ingredient extract in cosmetic preparation |
| CN105434310B (en) * | 2015-12-22 | 2021-02-19 | 广州市娇兰化妆品有限公司 | Application of periplaneta americana active ingredient extract in preparation of cosmetics |
| JP2022551319A (en) * | 2019-10-11 | 2022-12-08 | リャンシャン ジァネンダ バイオマテリアル ブリーディングシーオー．，エルティーディー． | American cockroach extract, formulation, preparation method and use thereof |
| JP7391204B2 (en) | 2019-10-11 | 2023-12-04 | リャンシャン ジァネンダ バイオマテリアル ブリーディングシーオー．，エルティーディー． | Extracts of American cockroach, formulations, preparation methods and uses thereof |
| CN111925408A (en) * | 2020-08-14 | 2020-11-13 | 云南中医药大学 | Preparation method of periplaneta americana polypeptide extract by using water as medium |
| CN111925408B (en) * | 2020-08-14 | 2023-06-06 | 云南中医药大学 | Preparation method of periplaneta americana polypeptide extract taking water as medium |
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Open date: 20070516 |