CN104337842A - Periplaneta americana medicine material and preparation method thereof - Google Patents
Periplaneta americana medicine material and preparation method thereof Download PDFInfo
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- CN104337842A CN104337842A CN201410354006.9A CN201410354006A CN104337842A CN 104337842 A CN104337842 A CN 104337842A CN 201410354006 A CN201410354006 A CN 201410354006A CN 104337842 A CN104337842 A CN 104337842A
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Abstract
The invention provides a preparation method of a periplaneta americana medicine material. The preparation method comprises the following steps: killing periplaneta americana in a non-heating manner, collecting bodies, and drying, thereby obtaining the periplaneta americana medicine material. The invention further provides the periplaneta americana medicine material prepared by using the method. The periplaneta americana medicine material is prepared in the non-heating manner, that is, an electric shock method, a suffocation method, a cold water drowning method, and the like, the content of extractum and free amino acid of the medicine material is greatly increased when being compared with that of the medicine material obtained by a conventional hot water scalding method, and the result shows that the preparation method of the medicine material is remarkably superior to a conventional process.
Description
Technical field
The present invention relates to a kind of periplaneta americana medical material and preparation method thereof.
Background technology
Periplaneta americana P erip laneta am ericana is Insecta Pterigota Blattaria Blattidae Periplaneta insecticide, and be commonly called as " Blatta seu periplaneta ", its beginning of being used as medicine is loaded in Shennong's Herbal.Numerous research shows, the chemical composition in periplaneta americana is mainly divided into two large classes.The first kind to be pheromone be large Lian has volatile pheromone component to release in vitro, mainly comprises sex pheromone and aggregation pheromone.Equations of The Second Kind is aminoacid and various insect neuropeptide, is be present in the chemical composition in its body.
Wherein, aminoacid forms organism protein and with the relevant the most basic material of vital movement, is the ultimate unit forming protein molecule in vivo, has close relationship with the vital movement of biology.It has special physiological function in antibody, is one of indispensable nutritional labeling in organism.Aminoacid is pharmaceutically being mainly used to prepare Hausmam Amin 20, also as medicine with for the synthesis of polypeptide drugs.Aminoacid as medicine has 100 kinds, has 20 kinds to have kind more than 100 with the aminoacid forming nonprotein comprising the aminoacid forming protein.
The compound preparation be made up of several amino acids occupies very important status in modern parenteral nutrition and " elemental diet " therapy, to the nutrition maintaining critical patient, rescue patient life has a positive effect, and becomes one of medical kind indispensable in modern medical service.
The periplaneta americana medical material tradition concocting method of current use is dead for adult bombay canary boiling water scalds, and dries or dries.But whether this traditional concocting method is applicable to periplaneta americana medical material, and current also rarely have research.
Summary of the invention
In the present invention with periplaneta americana medical material Determination of Free Amino Acids, extract content etc. for index, comparative evaluation is carried out to different concocting methods, to screening best concocting method.
The invention provides the preparation method of periplaneta americana medical material, it is by periplaneta americana, after adopting non-heated method to put to death, collects polypide, dry, obtains periplaneta americana medical material.
Wherein, described non-heated method is that electric shocking method, smother play or cold water are drowned method.
Further, described electric shocking method is that periplaneta americana is placed in high-voltage fence, puts to death under 2000 ~ 3000v voltage.
Further, described smother play is that periplaneta americana is placed in vacuum bag, and evacuation makes its death by suffocation.
Further, the described cold water method of being drowned periplaneta americana is placed in rustless steel to shine net, is immersed in cold water by solarization net and puts to death.
Wherein, described drying is cold drying, dries or air-dry to moisture≤10%.
The present invention's research shows, later stage baking temperature is 60 DEG C time, and the quality of periplaneta americana medical material prepared by the present invention is also better than traditional processing procedure.Therefore, the cold drying that the present invention uses, described cold drying, the baking temperature in traditional periplaneta americana concocting method can be continued to use---60 DEG C, also can carrying out drying lower than when 60 DEG C.
Present invention also offers periplaneta americana medical material prepared by said method.
Wherein, calculate with anhydrous periplaneta americana medical material, its ethanol-soluble extractives content is at more than 26%w/w, and free aminoacid content is at more than 2.0%w/w.
Further, calculate with anhydrous periplaneta americana medical material, its ethanol-soluble extractives content is 26-30%w/w, and free aminoacid content is 2.0-3.0%w/w.
Further, calculate with anhydrous periplaneta americana medical material, its ethanol-soluble extractives content is 26-29%w/w, and free aminoacid content is 2.3-2.6%w/w.
Present invention also offers the preparation be prepared from by above-mentioned periplaneta americana medical material, be with described periplaneta americana medical material (as periplaneta americana medicated powder) or its extract for active component, add the preparation that pharmaceutically conventional adjuvant or complementary composition are prepared from.
Pharmaceutically acceptable adjuvant of the present invention, refer to the material be included in addition to the active ingredient (s in dosage form, include but are not limited to filler (diluent), lubricant (fluidizer or antitack agent), dispersant, wetting agent, binding agent, regulator, solubilizing agent, antioxidant, antibacterial, emulsifying agent, disintegrating agent etc.Binding agent comprises syrup, arabic gum, gelatin, sorbitol, tragacanth, cellulose and its derivates (as microcrystalline Cellulose, sodium carboxymethyl cellulose, ethyl cellulose or hydroxypropyl methylcellulose etc.), gelatine size, syrup, starch slurry or polyvinylpyrrolidone etc.; Filler comprises lactose, Icing Sugar, dextrin, starch and derivant thereof, cellulose and its derivates, inorganic calcium salt (as calcium sulfate, calcium phosphate, calcium hydrogen phosphate, precipitated calcium carbonate etc.), sorbitol or glycine etc.; Lubricant comprises micropowder silica gel, magnesium stearate, Pulvis Talci, aluminium hydroxide, boric acid, hydrogenated vegetable oil, Polyethylene Glycol etc.; Disintegrating agent comprises starch and derivant (as carboxymethyl starch sodium, Explotab, pregelatinized Starch, modified starch, hydroxypropyl starch, corn starch etc.), polyvinylpyrrolidone or microcrystalline Cellulose etc.; Wetting agent comprises sodium lauryl sulphate, water or alcohol etc.; Antioxidant packages is containing sodium sulfite, sodium sulfite, sodium pyrosulfite, dibutyl benzoic acid etc.; Antibacterial comprises 0.5% phenol, 0.3% cresol, 0.5% chlorobutanol etc.; Regulator comprises hydrochloric acid, citric acid, potassium hydroxide (sodium), sodium citrate and buffer agent (comprising phosphoric acid dioxy sodium and sodium hydrogen phosphate) etc.; Emulsifier package containing Tween-80, do not have that sour Pyrusussuriensis is smooth, pluronic gram F-68, lecithin, fabaceous lecithin etc.; Solubilizing agent comprises tween 80, bile, glycerol etc.
Described pharmaceutically acceptable complementary composition, it has certain physiologically active, but adding of this composition can not change above-mentioned health product or the leading position of pharmaceutical composition in treatment of diseases, and only play auxiliary effect, these auxiliary effects are only the utilizations to this composition known activity, are the usual adjuvant treatment modality of field of medicaments.
The present invention adopts electric shocking method, smother play, cold water non-heated modes such as method of being drowned to carry out the preparation of periplaneta americana medical material, scald dead method with traditional boiling water to compare, in medical material, extractum and free aminoacid content obviously raise, and show that the preparation method of medical material of the present invention is significantly better than traditional handicraft.
Obviously, according to foregoing of the present invention, according to ordinary technical knowledge and the means of this area, not departing under the present invention's above-mentioned basic fundamental thought prerequisite, the amendment of other various ways, replacement or change can also be made.
Below by way of the form of specific embodiment, foregoing of the present invention is described in further detail again.But this should be interpreted as that the scope of the above-mentioned theme of the present invention is only limitted to following embodiment.All technology realized based on foregoing of the present invention all belong to scope of the present invention.
Detailed description of the invention
Embodiment 1 electric shocking method prepares periplaneta americana medical material
By adult bombay canary, be placed in high-voltage fence, energising, puts to death instantaneously, power-off under 2000 ~ 3000v voltage, collects polypide, is placed in heated-air circulation oven 60 DEG C and is dried to moisture≤10%.
Embodiment 2 smother play prepares periplaneta americana medical material
By adult bombay canary, be placed in vacuum bag, evacuation makes its death by suffocation, collects polypide, is placed in heated-air circulation oven 60 DEG C and is dried to moisture≤10%.
Embodiment 3 cold water is drowned legal system for periplaneta americana medical material
By adult bombay canary, be placed in stainless steel mesh, screen cloth be immersed in cold water, after its death, collect polypide, be placed in heated-air circulation oven 60 DEG C and be dried to moisture≤10%.
The research of embodiment 4 periplaneta americana medical material preparation method
1. periplaneta americana medicinal material processing
(1) electric shocking method
By adult bombay canary, be placed in high-voltage fence, energising, puts to death instantaneously, power-off under 2000 ~ 3000v voltage, collects polypide, is placed in heated-air circulation oven 60 DEG C and is dried to moisture≤10%.
(2) smother play
By adult bombay canary, be placed in vacuum bag, evacuation makes its death by suffocation, collects polypide, is placed in heated-air circulation oven 60 DEG C and is dried to moisture≤10%.
(3) cold water is drowned method
By adult bombay canary, be placed in stainless steel mesh, screen cloth be immersed in cold water, after its death, collect polypide, be placed in heated-air circulation oven 60 DEG C and be dried to moisture≤10%.
(4) boiling water scalds dead method
By adult bombay canary, be placed in the stainless-steel pan filling boiling water, scald dead, collect polypide, be placed in heated-air circulation oven 60 DEG C and be dried to moisture≤10%.
2. determination of water
The periplaneta americana medical material above method prepared, pulverizes with high speed disintegrator, crosses 40 mesh sieves, and sampling measures moisture according to aquametry (" Chinese Pharmacopoeia " annex Ⅸ H first method).
3. determination of extractives
Measure according to the hot dipping (" Chinese Pharmacopoeia " annex Ⅹ A) under ethanol-soluble extractives algoscopy item, use 50% ethanol as solvent, measure extract content.
4. Contents of Amino Acids
(1) sample preparation
Take Periplaneta Americana powder 10g, put in 250ml flask, add 8 times amount 65% alcohol reflux three times, each 1.5 hours, merge extractive liquid, filter, filtrate reduced in volume, to about 10ml, adds about 40ml boiling water, stirring and dissolving, leave standstill and let cool, divide the layer that deoils, filter, filtrate adding distil water is settled to 50ml.
(2) reference substance solution preparation
Precision takes the alanine reference substance 50mg being dried to constant weight at 105 DEG C, put in 100ml measuring bottle, is diluted to scale with water dissolution, shake up, precision measures 5ml, puts in 100ml measuring bottle, be diluted with water to scale, shake up, obtain (every 1ml is containing alanine 25 μ g).
(3) preparation of standard curve
Precision measures reference substance solution 0.0ml, 0.2ml, 0.4ml, 0.6ml, 0.8ml, 1.0ml, be placed in 10ml tool plug test tube respectively, add water to 1ml, add 0.2mol/L citrate buffer (pH value 5.0) 1.0ml, 1% ascorbic acid solution 0.1ml, 1,2,3-indantrione monohydrate ethylene glycol monomethyl ether test solution 3.0ml, shake up, put in boiling water bath and heat 15 minutes, take out, let cool, add 80% ethanol 3.0ml again, shake up, test according to spectrophotography (" Chinese Pharmacopoeia " annex V B), absorbance is measured at 570nm wavelength place, take absorbance as vertical coordinate, concentration is abscissa, drawing standard curve.
(4) total amino acids assay in sample
Precision measures sample 2.0ml, puts in 100ml measuring bottle, is diluted with water to scale, shake up.Precision measures 1ml, puts in 10ml tool plug test tube, the method under sighting target directrix curve preparation, from " adding 0.2mol/L citrate buffer (pH value 5.0) 1.0ml ", measure absorbance in accordance with the law, read amino acid whose content in need testing solution from standard curve, calculate, to obtain final product.
One, result
The periplaneta americana determination of extractives result of different concocting method is as follows:
Table 1
The periplaneta americana free aminoacid content of different concocting method is as follows:
Table 2
Four, conclusion
Adopt electric shocking method, smother play, cold water non-heated modes such as method of being drowned carry out periplaneta americana medicinal material processing, scald dead method with traditional boiling water and compare, in medical material, extractum and free aminoacid content obviously raise.
Claims (10)
1. the preparation method of periplaneta americana medical material, is characterized in that: it is by periplaneta americana, after adopting non-heated method to put to death, collects polypide, dry, obtains periplaneta americana medical material.
2. preparation method according to claim 1, is characterized in that: described non-heated method is that electric shocking method, smother play or cold water are drowned method.
3. preparation method according to claim 2, is characterized in that: described electric shocking method is that periplaneta americana is placed in high-voltage fence, puts to death under 2000 ~ 3000v voltage.
4. preparation method according to claim 2, is characterized in that: described smother play is that periplaneta americana is placed in vacuum equipment, and evacuation makes its death by suffocation.
5. preparation method according to claim 2, is characterized in that: the described cold water method of being drowned periplaneta americana is placed in rustless steel to shine net, is immersed in cold water by solarization net and puts to death.
6. preparation method according to claim 1, is characterized in that: described drying is cold drying, dry or air-dry to moisture≤10%.
7. the periplaneta americana medical material prepared of method described in claim 1 ~ 6 any one.
8. periplaneta americana medical material according to claim 7, is characterized in that: calculate with anhydrous periplaneta americana medical material, its ethanol-soluble extractives content is at more than 26%w/w, and free aminoacid content is at more than 2.0%w/w.
9. periplaneta americana medical material according to claim 8, is characterized in that: calculate with anhydrous periplaneta americana medical material, its ethanol-soluble extractives content is 26%-30%w/w, and free aminoacid content is 2.0%-3.0%w/w.
10. periplaneta americana medical material according to claim 9, is characterized in that: calculate with anhydrous periplaneta americana medical material, its ethanol-soluble extractives content is 26%-29%w/w, and free aminoacid content is 2.3%-2.6%w/w.
Priority Applications (1)
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| CN201410354006.9A CN104337842B (en) | 2013-08-02 | 2014-07-23 | A kind of American cockroach medicinal material and preparation method thereof |
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| CN201410354006.9A CN104337842B (en) | 2013-08-02 | 2014-07-23 | A kind of American cockroach medicinal material and preparation method thereof |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1961893A (en) * | 2005-11-08 | 2007-05-16 | 肖小芹 | Method for preparing extract of adult bombay canary |
| CN102846667A (en) * | 2012-08-23 | 2013-01-02 | 耿福能 | Preparation method for nanometer Periplaneta americana through low temperature drying |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1961893A (en) * | 2005-11-08 | 2007-05-16 | 肖小芹 | Method for preparing extract of adult bombay canary |
| CN102846667A (en) * | 2012-08-23 | 2013-01-02 | 耿福能 | Preparation method for nanometer Periplaneta americana through low temperature drying |
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Effective date of registration: 20190107 Address after: 615000 Xichang Airport Road, Sichuan Province Patentee after: Sichuan Good Doctor Panxi Pharmaceutical Co., Ltd. Address before: Room 206, Building 5, Gaopeng Avenue, Chengdu High-tech Zone, Sichuan 610041 Patentee before: Chengdu Baicao Heji Technology Co., Ltd. |
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