CN104352940A - Traditional Chinese medicinal composition for relieving physical fatigue - Google Patents
Traditional Chinese medicinal composition for relieving physical fatigue Download PDFInfo
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- CN104352940A CN104352940A CN201410685777.6A CN201410685777A CN104352940A CN 104352940 A CN104352940 A CN 104352940A CN 201410685777 A CN201410685777 A CN 201410685777A CN 104352940 A CN104352940 A CN 104352940A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/32—Bones; Osteocytes; Osteoblasts; Tendons; Tenocytes; Teeth; Odontoblasts; Cartilage; Chondrocytes; Synovial membrane
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/25—Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
- A61K36/258—Panax (ginseng)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/46—Eucommiaceae (Eucommia family), e.g. hardy rubber tree
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/54—Lauraceae (Laurel family), e.g. cinnamon or sassafras
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/74—Rubiaceae (Madder family)
- A61K36/744—Gardenia
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/74—Rubiaceae (Madder family)
- A61K36/746—Morinda
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/81—Solanaceae (Potato family), e.g. tobacco, nightshade, tomato, belladonna, capsicum or jimsonweed
- A61K36/815—Lycium (desert-thorn)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/906—Zingiberaceae (Ginger family)
- A61K36/9064—Amomum, e.g. round cardamom
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0053—Mouth and digestive tract, i.e. intraoral and peroral administration
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/08—Solutions
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
- A61K9/16—Agglomerates; Granulates; Microbeadlets ; Microspheres; Pellets; Solid products obtained by spray drying, spray freeze drying, spray congealing,(multiple) emulsion solvent evaporation or extraction
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/20—Pills, tablets, discs, rods
- A61K9/28—Dragees; Coated pills or tablets, e.g. with film or compression coating
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate
Abstract
The invention relates to a traditional Chinese medicinal composition for relieving physical fatigue. The traditional Chinese medicinal composition for relieving the physical fatigue is prepared from the following bulk pharmaceutical chemicals: cornua cervi pantotrichum, ginseng, eucommia bark, morinda officinalis, barbary wolfberry fruit, cinnamon, cape jasmine and fructus amomi. Part of medicinal materials are smashed in a certain ratio, the rest of the medicinal materials are extracted, concentrated, dried and smashed, and auxiliary materials are added, so that the traditional Chinese medicinal composition for relieving the physical fatigue is obtained.
Description
Technical field
The present invention relates to a kind of Chinese medicine composition of alleviating physical fatigue, particularly a kind of is Chinese medicine preparation compositions prepared by crude drug by Cornu Cervi Pantotrichum, Radix Ginseng, the Cortex Eucommiae, Radix Morindae Officinalis, Fructus Lycii, Cortex Cinnamomi, Fructus Gardeniae, Fructus Amomi.
Technical background
Along with the develop rapidly of modern society, the competitiveness of society is increasing, simultaneously due to the universal and nervous study and work etc. of computer, people are made to be subject to various intentional or unintentional sign stimulus, be subject to various tangible or invisible stress, subject motion or the work of various large amount of exercise, cause people to be often in the even overtired state of fatigue.If can not get timely rest after occurring tired, the time has been grown and will produce overwork and then cause going to bits.Except making a certain partial organ of health and system hypertonicity and causing various dissimilar disease damage, also there will be the hypofunction of circulation, breathing, digestive function etc.Tired impact also shows metabolic impact, and during musculation, K, P of the outer liquid of myocyte increase, and the distribution situation of cylinder electrolyte changes.The colloid substances deslagging be made up of mucin in urine increases, and in urine, the excretion of reducing substances and protein also increases.In a word, physiological change essence time tired is many-sided, as the accumulation of fatigue substances in body, comprises the metabolite etc. of lactic acid, acetone acid, liver glycogen, nitrogen; The imbalance of body fluid balance, comprises the balance etc. between osmotic pressure, pH value, redox materials.Show according to statistics, China more than half is at present in the state of chronic fatigue syndrome, account for 70%, and people more and more values in urban population healthy.
The core board alleviating physical fatigue sheet that converges contains the Cornu Cervi Pantotrichum of warming the kidney to activate YANG effect, the Cortex Eucommiae, Cortex Cinnamomi, Radix Morindae Officinalis, kidney tonifying, essence replenishing, nourishes edema with the liver involved and has Fructus Lycii; Regulating qi-flowing for strengthening spleen, reinforce functional activities of the heart have Radix Ginseng; Relieving QI stagnancy in the stomach has Fructus Amomi; For preventing Cornu Cervi Pantotrichum in prescription, the Cortex Eucommiae, Cortex Cinnamomi, Radix Morindae Officinalis too scorching, in side, add Fructus Gardeniae pathogenic fire purging relieving restlessness.Whole prescription has kidney tonifying, essence replenishing, invigorating the spleen and regulating the stomach, the effect of adjusting liver, taking good care of the mind of nourishing blood, and nurse one's health physical function comprehensively, can effectively alleviate because overworked or various stimulation causes physical fatigue, in side, composition cooperatively interacts and has synergistic function.
Summary of the invention
The present invention relates to a kind of alleviating physical fatigue Chinese medicine composition, be prepared from by the raw material of Chinese medicine medicine of following weight proportion, Cornu Cervi Pantotrichum 0.5-1.5 part, Radix Ginseng 2-6 part, Cortex Eucommiae 3-10 part, Radix Morindae Officinalis 3-8 part, Fructus Lycii 2-6 part, Cortex Cinnamomi 1-3 part, Fructus Gardeniae 1-3 part, Fructus Amomi 0.5-1.5 part.
Preferably, be prepared from by the raw material of Chinese medicine medicine of following weight proportion, 1 part, Cornu Cervi Pantotrichum, Radix Ginseng 4.4 parts, the Cortex Eucommiae 7 parts, Radix Morindae Officinalis 6.4 parts, Fructus Lycii 4 parts, Cortex Cinnamomi 2 parts, Fructus Gardeniae 2 parts, Fructus Amomi 1 part.
More than composition is by weight as proportioning, can increase according to corresponding proportion when producing or reduce, as large-scale production can by kilogram in units of, or in units of ton, small-scale production also can in units of milligram, weight can increase or reduce, but the constant rate of raw medicinal herbs weight proportion between each composition.
The ratio of above weight proportion is through that science screening obtains, and for especial patient, as serious symptom or mild, fat or modest patient, can the proportioning of amount of corresponding adjustment composition, and increase or reduce being no more than 100%, drug effect is constant.
Single medicinal material more than in composition, especially ministerial drug and adjuvant drug, also can be replaced by the suitable Chinese medicine with the identical property of medicine, its drug effect of the Chinese medicine preparation after replacement is constant.
Chinese medicine preparation compositions of the present invention, being that the raw material of Chinese medicine by being formed by above-mentioned formula is processed through extraction or other modes, making pharmaceutically active substance, subsequently, with this material for raw material, when needing, add medicine acceptable carrier, make according to the routine techniques of galenic pharmacy.Described active substance can obtain by extracting raw material of Chinese medicine respectively, also can be obtained by the common raw material of Chinese medicine that extracts, also can obtain by other means, as: by pulverizing, squeezing, calcine, grind, sieve, percolation, extraction, water extraction, alcohol extraction, ester carry, the material that method obtains, these active substances can be extractum form such as ketone is carried, chromatography, can be dry extract also can be fluid extract, need to determine to make different concentration according to the difference of preparation.
Pharmaceutically active substance in Chinese medicine preparation compositions of the present invention, its in the formulation shared percentage by weight can be 0.1-99.9%, all the other are medicine acceptable carrier.Pharmaceutical preparation of the present invention, exists in a unit, and described unit dosage form refers to the unit of preparation, as every sheet of tablet, and every capsules of capsule, every bottle of oral liquid, granule every bag etc.
Chinese medicine preparation of the present invention can be any pharmaceutically useful dosage form, and these dosage forms comprise: tablet, sugar coated tablet, film coated tablet, enteric coated tablet, capsule, hard capsule, soft capsule, oral liquid, suck agent, granule, electuary, pill, powder, unguentum, sublimed preparation, suspensoid, powder, solution, injection, suppository, ointment, plaster, cream, spray, drop, patch.Preparation of the present invention, preferably peroral dosage form, as: capsule, tablet, oral liquid, granule, pill, powder, sublimed preparation, unguentum etc.Most preferably tablet.
Chinese medicine preparation compositions of the present invention, the preparation of its oral administration can containing conventional excipient, and such as binding agent, filler, diluent, tablet agent, lubricant, disintegrating agent, coloring agent, flavoring agent and wetting agent, can carry out coating to tablet if desired.
The filler be suitable for comprises cellulose, mannitol, lactose and other similar filler.Suitable disintegrating agent comprises starch, polyvinylpyrrolidone and starch derivatives, such as sodium starch glycollate.Suitable lubricant comprises, such as magnesium stearate.The suitable acceptable wetting agent of medicine comprises sodium lauryl sulphate.
By mixing, fill, the method that tabletting etc. are conventional prepares solid oral composition.Repeatedly mix and active substance can be made to be distributed in those compositionss of a large amount of filler of whole use.
The form of oral liquid can be such as aqueous or oily suspensions, solution, Emulsion, syrup or elixir, or can be the composite dry products of a kind of available water before use or other suitable carrier.This liquid preparation can containing conventional additive, such as suspending agent, such as sorbitol, syrup, methylcellulose, gelatin, hydroxyethyl-cellulose, carboxymethyl cellulose, aluminium stearate gel or hydrogenated edible fats, emulsifying agent, such as lecithin, anhydro sorbitol monooleate or arabic gum; Non-aqueous carrier (they can comprise edible oil), the oily ester of the such as ester of almond oil, fractionated coconut oil, such as glycerol, propylene glycol or ethanol; Antiseptic, such as para hydroxybenzene methyl ester or propyl p-hydroxybenzoate or sorbic acid, and if need, can containing conventional flavouring agent or coloring agent.
For injection, the fluid unit dosage form of preparation contains active substance of the present invention and sterile carrier.According to carrier and concentration, this compound can be suspended or dissolve.The preparation of solution is normally by being dissolved in a kind of carrier by active substance, filter-sterilized before being loaded a kind of suitable bottle or ampoule, then seals.Adjuvant such as a kind of local anesthetic, antiseptic and buffer agent also can be dissolved in this carrier.In order to improve its stability, by freezing for this compositions after loading bottle, and under vacuo water can be removed.
Chinese medicine preparation of the present invention, applicable medicine acceptable carrier is optionally added when being prepared into medicament, described medicine acceptable carrier is selected from: mannitol, sorbitol, sodium pyrosulfite, sodium sulfite, sodium thiosulfate, cysteine hydrochloride, TGA, methionine, vitamin C, EDETATE SODIUM, Ethylenediaminetetraacetic Acid Calcium Salt, the alkali-metal carbonate of monovalence, acetate, phosphate or its aqueous solution, hydrochloric acid, acetic acid, sulphuric acid, phosphoric acid, aminoacid, sodium chloride, potassium chloride, sodium lactate, xylitol, maltose, glucose, fructose, dextran, glycine, starch, sucrose, lactose, mannitol, silicon derivative, cellulose and its derivates, alginate, gelatin, polyvinylpyrrolidone, glycerol, Tween 80, agar, calcium carbonate, calcium bicarbonate, surfactant, Polyethylene Glycol, cyclodextrin, beta-schardinger dextrin-, phospholipid material, Kaolin, Pulvis Talci, calcium stearate, magnesium stearate etc.
Preparation of the present invention according to the situation determination usage and dosage of patient, can take three every day in use, each 1-20 agent, as: 1-20 bag or grain or sheet.
Chinese medicine preparation compositions of the present invention can adopt following methods to prepare:
Cornu Cervi Pantotrichum, part Radix Ginseng are pulverized, residue medical material water extraction, leaching medicinal liquid; Filtrate merges, concentrated, dry, adds medicated powder for subsequent use, adjuvant makes preparation.
Preferably, the preparation method of alleviating physical fatigue Chinese medicine preparation of the present invention, it is characterized in that, step is as follows: Cornu Cervi Pantotrichum, part Radix Ginseng are pulverized, residue medicinal material extract 2 times, and first time, by 8 times of weight water, extracts 1.5 hours; Second time, with 6 times of water gagings, is extracted 1 hour, and filtrate merges concentrated, dry, pulverizing, obtains pharmaceutically active substance, with this active substance for active constituents of medicine, be prepared into alleviating physical fatigue Chinese medicine preparation according to galenic pharmacy routine techniques through process.Wherein said drying means is preferably not higher than microwave drying under 60 DEG C of conditions.
Preparation of the present invention has the effect of alleviating physical fatigue, may be used for alleviating physical fatigue.In this product, Fructus Lycii, Cortex Cinnamomi, Fructus Gardeniae, Fructus Amomi etc. are all in national medicine-food two-purpose kind list, Cornu Cervi Pantotrichum, Radix Ginseng, the Cortex Eucommiae, Morinda can be used for the kind of health food in national publication, and medical starch, pregelatinized Starch, low-substituted hydroxypropyl methylcellulose, magnesium stearate belong to common dosage forms adjuvant.Without incompatibility between former, adjuvant, historical usage proved kind conventional amount used use safety, in side, composition cooperatively interacts and has synergistic function.
The preferred formula of the present invention is: 1 part, Cornu Cervi Pantotrichum, Radix Ginseng 4.4 parts, the Cortex Eucommiae 7 parts, Radix Morindae Officinalis 6.4 parts, Fructus Lycii 4 parts, Cortex Cinnamomi 2 parts, Fructus Gardeniae 2 parts, Fructus Amomi 1 part; More than form,
If 1 part is 100g, can be made into pharmaceutical preparation 1000 doses.The final drug preparation that described dose of finger is made, as capsule 1000 doses refers to capsule preparations 1000,1000 doses, tablet refers to 1000, tablet, is granule 1000 grams for granule.Weight can increase or reduce,
But the constant rate of the raw medicinal herbs weight proportion between each composition.
Beneficial effect of the present invention is further illustrated below by way of experimental data:
Preparation method of the present invention is through screening acquisition, and screening process is as follows:
Due to the equal water soluble of the most of effective ingredient of square Chinese crude drug, therefore extract solvent and be decided to be water, ginsenoside alleviates one of physical resisting fatigue principle active component, total saponins based on ginsenoside is inspection target, investigate extraction time, amount of water, extraction time to the impact of total saponins, determine best extraction process.The results are shown in Table 1, table 2, table 3.
Table 1 orthogonal test gauge outfit L
9(3
4)
Get medical material except beating powder, get 1/4 recipe quantity (645g) medical material 9 parts, test by orthogonal test arrangement, filter, filtrate reduced in volume, dry, obtain 9 groups of sample dry extracts.According to (the total saponins assay method that health food functional component detection method specifies carries out.) measure total soap amount in extractum, the results are shown in Table 2.
Table 2 orthogonal experiments
The variance analysis of table 3 total saponins
α＝0.05
Note: total saponins %=records total saponins/(medical material amount * dry spun)
Results and analysis: experimental result display extraction time extracts total saponins appreciable impact, extraction time, amount of water extract total saponins and do not make significant difference under the condition of design, according to intuitive analysis, optimum extraction condition is: material of getting it filled, extracting in water 3 times, each extraction 1.5 hours, adds 10 times of water gagings.According to intuitive analysis, extract for three times and increase by 7% than extracted twice total saponins amount, 1.5 hours extraction times comparatively extracted 1 hour total saponins amount increases the comparatively 8 times amount amount of water total saponins increases by 6% of 1%, 10 times amount amount of water.Self 2 times of water gaging can be absorbed from the saving energy and preliminary experiment medical material itself to consider, extraction process is modified to: all the other Six-element medical materials, decoct with water secondary, first time adds the soak by water 1.5 hours of 8 times amount, second time adds 6 times amount soak by water 1 hour, filters, merging filtrate.
Demonstration test
Get prescription medical material 1.29kg, according to determining that extraction process carries out 3 checkings, getting part extract dry, calculating the result in table 4.
Table 4 extracts demonstration test result
| Tested number | Yield of extract (%) | Total saponins (%) | Total saponins (g) |
| 1 | 12.87 | 3.15 | 5.23 |
| 2 | 13.17 | 3.19 | 5.40 |
| 3 | 13.33 | 3.24 | 5.57 |
Drying condition screens
Conventional extract dry mode has vacuum drying, microwave drying, constant pressure and dry etc. at present.Demonstration test gained extractum is mixed, dry respectively, measure total saponin content, the results are shown in Table 5:
Table 5 drying condition screens
| Vacuum drying | Microwave drying | Constant pressure and dry | |
| Baking temperature | 60 | 60 | 60 |
| Extractum (g) | 302.7 | 298.5 | 314.2 |
| Dry cream amount (g) | 144.9 | 142.2 | 149.9 |
| Dry cream rate (%) | 12.62 | 12.56 | 12.58 |
| Total saponins (%) | 3.25 | 3.23 | 3.20 |
| Total saponins (g) | 15.87 | 15.70 | 15.57 |
| Drying time (h) | 4 | 1.6 | 6 |
According to conventional adjuvant, investigate the adjuvants such as carboxymethyl starch sodium, starch, microcrystalline Cellulose (101), calcium hydrogen phosphate, pregelatinized Starch, low-substituted hydroxypropyl methylcellulose, magnesium stearate, design prescription, with broken brittleness, disintegration for investigating target, screening prescription.
Table 6 prescription screening table
In order to better control the curative effect of product, having formulated the content assaying method of total saponins in side, being specially, ultraviolet visible spectrophotometry, determined wavelength is 560 ± 2nm.
Instrument and reagent
Instrument lambda25 spectrophotometry instrument;
Reagent alleviating physical fatigue preparation, ginsenoside re reference substance (Nat'l Pharmaceutical & Biological Products Control Institute provides, for assay); Methanol, ethanol are analytical pure, and water is ultra-pure water, XAD-2 macroporous resin, neutral alumina (100-200 order).
Sample treatment
Accurately take 1g sample, put in 100ml volumetric flask, add a small amount of water, ultrasonic 30min, use water standardize solution, shake up after cooling, place, Aspirate supernatant 1.0ml carries out column chromatography.
Make chromatography pipe with 10ml syringe, in-built 3cmXAD-2 macroporous resin, above add 1cm neutral alumina (100-200 order).First wash post with 25ml70% ethanol, then wash post with 25ml, discard eluent.
Accurately add the processed good sample solution of 1.0ml, wash post with 25ml, discard eluent.With 25ml70% ethanol elution ginsenoside, collect eluent in evaporating dish, put 60 DEG C of water-baths and volatilize, do colour developing with this.
Colour developing
0.2ml5% vanillin glacial acetic acid solution is accurately added in the above-mentioned evaporating dish volatilized, residue is dissolved, add 0.8ml perchloric acid again, shake up rear immigration color comparison tube, 60 DEG C of heating in water bath 10min, take out, after ice bath cooling, accurately add glacial acetic acid 5.0ml, with zero pipe for blank, shake up rear 1cm cuvette, under 560nm wavelength, measure absorbance together with standard pipe.
Standard pipe
Accurate absorption ginsenoside Re standard solution (2mg/ml) 100ul is put in evaporating dish, and water-bath volatilizes (lower than 60 DEG C), by development step, under 560nm wavelength, measures absorbance.
Result calculates
Total saponins (g/100g)=(A
sample/ A
mark) × C
mark× (V
sample/ m
sample) × 100/1000/1000
A in formula---absorbance
M---sample quality, g
V---dilution volume, ml
C---the amount of standard pipe ginsenoside Re, ug
Note: result of calculation retains two position effective digitals
Beneficial effect of the present invention is further illustrated below by way of experimental data:
Test method
1. swimming with a load attached to the body experiment: the sheet lead of 5% body weight that mouse tail is born a heavy burden, be placed in 50cm × 50cm × 40cm pond went swimming, depth of water 40cm, water temperature 25 DEG C ± 0.5 DEG C, 5, every pond mice, the swimming of record mice started to the death time as the mice burden swimming time.
2. hepatic glycogen measures: cervical dislocation puts to death mice, gets liver, homogenate, by the operation of glycogen testing cassete description, measures Mouse Liver glycogen content.
3. blood lactase acid measures: after last gives given the test agent 30min, get blood 0.1mL from mouse orbit venous plexus.Then make it not bear a heavy burden to stop after the swimming trunk went swimming 10min of water temperature 30 DEG C, respectively at after swimming 0, respectively get blood 0.1mL again after 20min, by the operation of full blood lactic testing cassete description, measure each pipe absorbance, calculate Serum lactic acid content and blood lactase acid area under curve.Blood lactase acid area under curve=5 × (the blood lactase acid value of the rear 20min of blood lactase acid value+2 × swimming rest of the rear 0min of front blood lactase acid value+3 × swimming that swims).
The medicine that Experimental agents uses the embodiment of the present invention 1 to prepare, is called: remittance core board alleviating physical fatigue preparation
1 converges core board alleviating physical fatigue preparation to the impact of mice muscle power
Table 7 is visible, and the walking weight load of tested object height, middle dosage group mice is obviously longer than blank group, and difference has significant (P<0.05).Show that this tested material has the effect extending the mice burden swimming time, can judge that remittance core board alleviating physical fatigue preparation has effect of relieving physical fatigue.
Table 7 converges core board alleviating physical fatigue preparation to the impact of mice burden swimming time
Note: each dosage group compares with blank group, * * P < 0.01.
2 converge core board alleviating physical fatigue preparation to the impact of hepatic glycogen
From table 8, the hepatic glycogen content of tested material each dosage group mice is all higher than blank group, and difference has statistical significance (P<0.01).Show that this tested material has the effect promoting mice liver glycogen reserves, judge that this experimental result is positive.
Table 8 converge core board alleviating physical fatigue sheet on the impact of Mouse Liver glycogen content (
)
Note: each dosage group compares with blank group, * * P < 0.01.
3 converge core board alleviating physical fatigue preparation to the impact of blood lactase acid
From table 9, the each dosage group of tested material on mice swimming before blood lactase acid value there are no significant impact (P>0.05), to blood lactase acid value after mice swimming lower than blank group, but no significant difference (P>0.05); High, middle dosage group obviously can reduce the blood lactase acid value after mice swimming rest 20min, and compare with blank group, difference has significant (P<0.05).Tested object height, middle dosage group Mouse Blood lactic acid area under curve are lower than blank group, difference has significant (P<0.05), show that this tested material has and reduce the effect that lactic acid generates after mouse movement, judge that this experimental result is positive.
Table 9 converge core board alleviating physical fatigue sheet on the impact of Mouse Blood Lactate and area under curve (
)
Note: each dosage group compares with blank group, * P < 0.05.
Detailed description of the invention:
Further illustrate the present invention below in conjunction with embodiment, but do not show limiting to the claimed invention.
Embodiment 1
The preparation of tablet
Get Cornu Cervi Pantotrichum 100g, Radix Ginseng 440g, Cortex Eucommiae 700g, Radix Morindae Officinalis 640g part, Fructus Lycii 400g, Cortex Cinnamomi 200g, Fructus Gardeniae 200g, Fructus Amomi 100g.
Cornu Cervi Pantotrichum, part Radix Ginseng are ground into 80 orders, for subsequent use, residue seven flavor medicine material, decoct with water secondary, first time adds the soak by water 1.5 hours of 8 times amount, second time adds 6 times amount soak by water 1 hour, filter, filtrate merges, add above-mentioned filtrate for subsequent use, concentrating under reduced pressure becomes relative density to be the thick paste of 1.1 ~ 1.2 (60 DEG C), 55 DEG C ~ 60 DEG C microwave drying, pulverize, add medicated powder after pulverizing, recipe quantity pregelatinized Starch, low-substituted hydroxypropyl methylcellulose and low-substituted hydroxypropyl methylcellulose, mix homogeneously, add 75% ethanol 40g, granulate with 24 mesh sieves, 65 DEG C ~ 70 DEG C oven dryings, 20 mesh sieve granulate, add magnesium stearate, mix homogeneously, tabletting, film coating, obtain.
Embodiment 2
Get 1 part, Cornu Cervi Pantotrichum, Radix Ginseng 4.4 parts, the Cortex Eucommiae 7 parts, Radix Morindae Officinalis 6.4 parts, Fructus Lycii 4 parts, Cortex Cinnamomi 2 parts, Fructus Gardeniae 2 parts, Fructus Amomi 1 part.
Wherein 1 part is 100g, extracts pharmaceutically active substance, be prepared into capsule according to galenic pharmacy routine techniques according to the method for embodiment 1.
Embodiment 3
Get 1 part, Cornu Cervi Pantotrichum, Radix Ginseng 4.4 parts, the Cortex Eucommiae 7 parts, Radix Morindae Officinalis 6.4 parts, Fructus Lycii 4 parts, Cortex Cinnamomi 2 parts, Fructus Gardeniae 2 parts, Fructus Amomi 1 part.
Wherein 1 part is 100g, extracts pharmaceutically active substance, be prepared into granule according to galenic pharmacy routine techniques according to the method for embodiment 1.
Embodiment 4
Get 1 part, Cornu Cervi Pantotrichum, Radix Ginseng 4.4 parts, the Cortex Eucommiae 7 parts, Radix Morindae Officinalis 6.4 parts, Fructus Lycii 4 parts, Cortex Cinnamomi 2 parts, Fructus Gardeniae 2 parts, Fructus Amomi 1 part.
Wherein 1 part is 100g, extracts pharmaceutically active substance, be prepared into oral solutions according to galenic pharmacy routine techniques according to the method for embodiment 1.
Embodiment 5
Cornu Cervi Pantotrichum, part Radix Ginseng are ground into 80 orders, for subsequent use, residue seven flavor medicine material, decoct with water secondary, first time adds the soak by water 1.5 hours of 8 times amount, second time adds 6 times amount soak by water 1 hour, filter, filtrate merges, add above-mentioned filtrate for subsequent use, concentrating under reduced pressure becomes relative density to be the thick paste of 1.20 ~ 1.35 (60 DEG C), 55 DEG C ~ 60 DEG C microwave drying, pulverize, add medicated powder for subsequent use, recipe quantity pregelatinized Starch, starch and low-substituted hydroxypropyl methylcellulose, mix homogeneously, add 75% ethanol 40g, granulate with 24 mesh sieves, 65 DEG C ~ 70 DEG C oven dryings, 20 mesh sieve granulate, add magnesium stearate, mix homogeneously, tabletting, film coating, obtain.
Claims (6)
1. the Chinese medicine composition of an alleviating physical fatigue, it is characterized in that: be prepared from by the raw material of Chinese medicine medicine of following weight proportion, Cornu Cervi Pantotrichum 0.5-2 part, Radix Ginseng 2-6 part, Cortex Eucommiae 3-10 part, Radix Morindae Officinalis 3-8 part, Fructus Lycii 2-6 part, Cortex Cinnamomi 1-3 part, Fructus Gardeniae 1-3 part, Fructus Amomi 0.5-2 part.
2. Chinese medicine composition according to claim 1, it is characterized in that: be prepared from by the raw material of Chinese medicine medicine of following weight proportion, Cornu Cervi Pantotrichum 1-1.5 part, Radix Ginseng 4-5 part, Cortex Eucommiae 6-7 part, Radix Morindae Officinalis 6-7 part, Fructus Lycii 4-5 part, Cortex Cinnamomi 1-2 part, Fructus Gardeniae 1-2 part, Fructus Amomi 1-1.5 part.
3. the Chinese medicine composition described in claim 1-2, is characterized in that, Cornu Cervi Pantotrichum described in formula, Radix Ginseng, the Cortex Eucommiae, Radix Morindae Officinalis, Fructus Lycii, Cortex Cinnamomi, Fructus Gardeniae, Fructus Amomi is all or part of feeds intake with its extract.
4. alleviating physical fatigue preparation according to claim 1-3, is characterized in that, makes any one dosage form by conventional method.
5. the alleviating physical fatigue preparation according to claim 1-3, makes tablet, capsule, oral liquid, granule, pill, powder, unguentum, sublimed preparation, suspensoid, powder, solution, suppository, unguentum, drop pill or patch.
6. the application of Chinese medicine composition in the medicine preparing alleviating physical fatigue or health product of claim 1.
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| CN104719745A (en) * | 2015-03-27 | 2015-06-24 | 张青杰 | Five-element plant powder (white) food formula and preparation technology of five-element plant powder (white) food |
| CN114099625A (en) * | 2021-10-27 | 2022-03-01 | 上海纳米技术及应用国家工程研究中心有限公司 | Formula for improving sexual life quality reduction and preparation process thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104719745A (en) * | 2015-03-27 | 2015-06-24 | 张青杰 | Five-element plant powder (white) food formula and preparation technology of five-element plant powder (white) food |
| CN114099625A (en) * | 2021-10-27 | 2022-03-01 | 上海纳米技术及应用国家工程研究中心有限公司 | Formula for improving sexual life quality reduction and preparation process thereof |
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Address after: 330043 No. 1266 Huiren Avenue, Xiaolan Economic and Technological Development Zone, Nanchang City, Jiangxi Province Co-patentee after: Shanghai Zhongchuang Medicine Science Co., Ltd. Patentee after: Jiangxi Huiren pharmaceutical Limited by Share Ltd Address before: 330052 1189 Yingbin Avenue, Nanchang, Jiangxi Co-patentee before: Shanghai Zhongchuang Medicine Science Co., Ltd. Patentee before: Huiren Pharmaceutical Co., Ltd. Jiangxi |
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