CN104352940B - A kind of Chinese medicine composition for alleviating physical fatigue - Google Patents
A kind of Chinese medicine composition for alleviating physical fatigue Download PDFInfo
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- CN104352940B CN104352940B CN201410685777.6A CN201410685777A CN104352940B CN 104352940 B CN104352940 B CN 104352940B CN 201410685777 A CN201410685777 A CN 201410685777A CN 104352940 B CN104352940 B CN 104352940B
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/32—Bones; Osteocytes; Osteoblasts; Tendons; Tenocytes; Teeth; Odontoblasts; Cartilage; Chondrocytes; Synovial membrane
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/25—Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
- A61K36/258—Panax (ginseng)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/46—Eucommiaceae (Eucommia family), e.g. hardy rubber tree
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/54—Lauraceae (Laurel family), e.g. cinnamon or sassafras
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/74—Rubiaceae (Madder family)
- A61K36/744—Gardenia
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/74—Rubiaceae (Madder family)
- A61K36/746—Morinda
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/81—Solanaceae (Potato family), e.g. tobacco, nightshade, tomato, belladonna, capsicum or jimsonweed
- A61K36/815—Lycium (desert-thorn)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/906—Zingiberaceae (Ginger family)
- A61K36/9064—Amomum, e.g. round cardamom
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0053—Mouth and digestive tract, i.e. intraoral and peroral administration
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/08—Solutions
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
- A61K9/16—Agglomerates; Granulates; Microbeadlets ; Microspheres; Pellets; Solid products obtained by spray drying, spray freeze drying, spray congealing,(multiple) emulsion solvent evaporation or extraction
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/20—Pills, tablets, discs, rods
- A61K9/28—Dragees; Coated pills or tablets, e.g. with film or compression coating
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate
Abstract
The present invention relates to a kind of Chinese medicine composition for alleviating physical fatigue, it is bulk drug, by a certain percentage, part pulverizing medicinal materials by pilose antler, ginseng, the bark of eucommia, Morinda officinalis, the fruit of Chinese wolfberry, Chinese cassia tree, cape jasmine, fructus amomi, remaining medicinal material adds auxiliary material and is made by extraction, concentration, dry, crushing.
Description
Technical field
The present invention relates to a kind of Chinese medicine composition for alleviating physical fatigue, it is more particularly to a kind of by pilose antler, ginseng, the bark of eucommia,
Morinda officinalis, the fruit of Chinese wolfberry, Chinese cassia tree, cape jasmine, fructus amomi are Chinese medicine preparation composition prepared by bulk drug.
Technical background
With the rapid development of modern society, social competitiveness is increasing, simultaneously because the popularization of computer and tight
Study and work opened etc., people are made to be pressed by various intentional or unintentional sign stimuluses by various tangible or invisible spirit
Power, the motion or work of various large amount of exercise are subject, cause people often in fatigue even fatigue state.Fatigue goes out
If cannot be rested in time after now, the time, which has been grown to produce, overworks and then causes to go to bits.Except making a part of device of body
Official and system is overstretched causes outside various types of disease damage, also occurs that the function of circulation, breathing, digestive function etc. subtracts
Move back.Influences of fatigue is also manifested by the influence to metabolism, and K, P increase of the outer liquid of myocyte, are electrolysed in vivo during muscle activity
The distribution situation of matter changes.The colloid substances deslagging being made up of in urine mucoprotein increases, reducing substances and protein in urine
Excretion also increase.In a word, physiological change essence when tired is many, such as accumulation of internal fatigue substances, including breast
Acid, pyruvic acid, liver glycogen, the metabolite etc. of nitrogen；Between the imbalance of isohydria, including osmotic pressure, pH value, redox materials
Balance etc..Show according to statistics, China more than half at present is in the state of chronic fatigue syndrome, in urban human
70% is accounted in mouthful, and people increasingly value health.
The benevolence board of converging alleviates pilose antler, the bark of eucommia, Chinese cassia tree, the Morinda officinalis that physical fatigue piece contains warming kidney and enhancing yang effect, kidney tonifying, essence replenishing,
Nourishing edema with the liver involved has the fruit of Chinese wolfberry；Regulating qi-flowing for strengthening spleen, the help motive have ginseng；Relieving QI stagnancy in the stomach has fructus amomi；To prevent pilose antler in prescription, Du
Secondary, Chinese cassia tree, Morinda officinalis are excessively scorching, and cape jasmine purging intense heat relieving restlessness is added in side.Whole prescription has kidney tonifying, essence replenishing, strengthening the spleen and stomach, blood-nourishing
Adjust liver, raise the effect of the mind, to the comprehensive conditioning of physical function, can effectively alleviate because overworked or various stimulations cause body
Power is tired, and composition, which cooperates, in side has synergistic function.
The content of the invention
The present invention relates to one kind to alleviate physical fatigue Chinese medicine composition, by the Chinese medicine material medicine preparation of following weight proportion
Into pilose antler 0.5-1.5 parts, ginseng 2-6 parts, bark of eucommia 3-10 parts, Morinda officinalis 3-8 parts, fruit of Chinese wolfberry 2-6 parts, Chinese cassia tree 1-3 parts, cape jasmine
1-3 parts, fructus amomi 0.5-1.5 parts.
Preferably, be prepared by the Chinese medicine material medicine of following weight proportion, 1 part of pilose antler, 4.4 parts of ginseng, 7 parts of the bark of eucommia,
6.4 parts of Morinda officinalis, 4 parts of the fruit of Chinese wolfberry, 2 parts of Chinese cassia tree, 2 parts of cape jasmine, 1 part of fructus amomi.
Composition described above is by weight as proportioning, can increase or reduce according to corresponding proportion in production, such as big rule
Mould production can by kilogram in units of, or in units of ton, small-scale production can also be in units of milligram, and weight can increase
Or reduce, but the constant rate of the raw medicinal herbs weight proportion between each composition.
The ratio of above weight proportion screens to obtain by science, fat such as severe or mild for especial patient
Or the patient of slight of stature, the proportioning of the amount of composition can be accordingly adjusted, is increased or decreased no more than 100%, drug effect is constant.
Single medicinal material in composition described above, especially ministerial drug and adjutant, can also be had by appropriate in the identical property of medicine
Medicine is replaced, and Chinese medicine preparation its medicine effect after replacement is constant.
The Chinese medicine preparation composition of the present invention, it is by extraction or its other party by the Chinese medicine material for forming above-mentioned formula
Formula process, pharmaceutically active substance is made, then, using the material as raw material, it is necessary to when add pharmaceutically acceptable carrier, according to system
Made of the routine techniques of agent.The active material can be obtained by extracting Chinese medicine material respectively, can also be by common
Extraction Chinese medicine material obtains, and can also obtain by other means, such as：By crushing, squeezing, calcining, grinding, sieving, diacolation,
Extraction, water extraction, alcohol extracting, the material that ester carries, ketone carries, the methods of chromatographing obtains, these active materials can be medicinal extract form, can be with
It is that dry extract can also be liquid extract, different according to preparation need to determine that different concentration is made.
Pharmaceutically active substance in the Chinese medicine preparation composition of the present invention, shared percentage by weight can be in the formulation for it
0.1-99.9%, remaining is pharmaceutically acceptable carrier.The pharmaceutical preparation of the present invention, exists, the list in a unit
Position dosage form refers to the unit of preparation, such as every of tablet, every capsule of capsule, and every bottle of oral liquid, every bag of granule
Deng.
The Chinese medicine preparation of the present invention can be any pharmaceutically useful formulation, and these formulations include：It is tablet, sugar coated tablet, thin
Film coated tablet, enteric coated tablet, capsule, hard capsule, soft capsule, oral liquid, mouth containing agent, granule, electuary, pill,
Powder, paste, sublimed preparation, supensoid agent, pulvis, solution, injection, suppository, ointment, emplastrum, creme, spray, drops,
Patch.The preparation of the present invention, preferably peroral dosage form, such as：Capsule, tablet, oral liquid, granule, pill, powder, pellet
Agent, paste etc..Most preferably tablet.
The Chinese medicine preparation composition of the present invention, its preparation being administered orally containing conventional excipient, such as adhesive,
Filler, diluent, tablet agent, lubricant, disintegrant, colouring agent, flavor enhancement and wetting agent, tablet can be wrapped if necessary
Clothing.
Applicable filler includes cellulose, mannitol, lactose and other similar fillers.Suitable disintegrant bag
Include starch, polyvinylpyrrolidone and starch derivatives, such as sodium starch glycollate.Suitable lubricant includes, such as firmly
Fatty acid magnesium.Suitable pharmaceutically acceptable wetting agent includes lauryl sodium sulfate.
Solid oral composition can be prepared by conventional methods such as mixing, filling, tablettings.Work can be made by carrying out mixing repeatedly
Property material be distributed in entirely using a large amount of fillers those compositions in.
The form of oral liquid for example can be water-based or oily suspensions, solution, emulsion, syrup or elixir,
Or can be a kind of dry products that water or other suitable carriers can be used to compound before use.This liquid preparation can contain
Conventional additive, such as suspending agent, such as sorbierite, syrup, methylcellulose, gelatin, hydroxyethyl cellulose, carboxymethyl are fine
Dimension element, aluminium stearate gel or hydrogenated edible fats, emulsifying agent, such as lecithin, anhydro sorbitol monooleate or Arab
Glue；Non-aqueous carrier (they can include edible oil), such as the oily ester of the ester of apricot kernel oil, fractionated coconut oil, such as glycerine,
Propane diols or ethanol；Preservative, such as para hydroxybenzene methyl esters or propylparaben or sorbic acid, and if desired,
Contain conventional flavouring agent or colouring agent.
For injection, the fluid unit dosage form of preparation contains the active material and sterile carrier of the present invention.According to carrier
And concentration, this compound can be suspended or be dissolved.The preparation of solution is dissolved in a kind of load typically by by active material
In body, sterilization is filtered before a kind of suitable bottle or ampoule is loaded into, is then sealed.For example a kind of local anaesthesia of auxiliary material
Agent, preservative and buffer can also be dissolved in this carrier., can be after bottle be loaded by this in order to improve its stability
Kind composition frost, and under vacuo remove water.
The Chinese medicine preparation of the present invention, suitable pharmaceutically acceptable carrier is optionally added when being prepared into medicament,
The pharmaceutically acceptable carrier is selected from：Mannitol, sorbierite, sodium pyrosulfite, sodium hydrogensulfite, sodium thiosulfate, hydrochloric acid
Cysteine, TGA, methionine, injection Vitamin B_6 DTA disodiums, Ethylenediaminetetraacetic Acid Calcium Salt, the carbonate of monovalence alkali metal, acetate,
Phosphate or its aqueous solution, hydrochloric acid, acetic acid, sulfuric acid, phosphoric acid, amino acid, sodium chloride, potassium chloride, sodium lactate, xylitol, malt
Sugar, glucose, fructose, dextran, glycine, starch, sucrose, lactose, mannitol, silicon derivative, cellulose and its spread out
Biology, alginates, gelatin, polyvinylpyrrolidone, glycerine, Tween 80, agar, calcium carbonate, calcium bicarbonate, surfactant,
Polyethylene glycol, cyclodextrin, beta-schardinger dextrin, phospholipid material, kaolin, talcum powder, calcium stearate, magnesium stearate etc..
The preparation of the present invention determines usage and dosage according to the situation of patient when in use, can often taken three times per day, each 1-20
Agent, such as：1-20 bags or grain or piece.
The Chinese medicine preparation composition of the present invention can use following methods to prepare：
Pilose antler, part ginseng are crushed, and remaining medicinal material is extracted with water, leaching decoction；Filtrate merges, and concentrates, and dries, and adds standby
Preparation is made in medication powder, auxiliary material.
Preferably, the preparation method of alleviation physical fatigue Chinese medicine preparation of the invention, it is characterised in that step is as follows：Deer
Fine and soft, part ginseng crushes, and remaining medicinal material extract 2 times, for the first time with 8 times of weight waters, extracts 1.5 hours；For the second time with 6 times of amounts
Water, extract 1 hour, filtrate merges concentration, dries, crushes, and pharmaceutically active substance is obtained by processing, using the active material as medicine
Thing active component, it is prepared into according to galenic pharmacy routine techniques and alleviates physical fatigue Chinese medicine preparation.Wherein described drying means is preferred
For microwave drying under the conditions of not higher than 60 DEG C.
The preparation of the present invention has the function that to alleviate physical fatigue, can be used for alleviating physical fatigue.The fruit of Chinese wolfberry in this product,
Chinese cassia tree, cape jasmine, fructus amomi etc. are in national medicinal and edible varieties list, and pilose antler, ginseng, the bark of eucommia, Morinda are in national publication
Available for the kind of health food, medical starch, pregelatinized starch, low-substituted hydroxypropyl methylcellulose, magnesium stearate belong to normal
Use pharmaceutical adjunct.Without incompatibility between former, auxiliary material, historical usage confirms that this kind conventional amount used is safe to use, is formed in side
Mutual cooperation has synergistic function.
Currently preferred formula is：1 part of pilose antler, 4.4 parts of ginseng, 7 parts of the bark of eucommia, 6.4 parts of Morinda officinalis, 4 parts of the fruit of Chinese wolfberry, meat
2 parts of osmanthus, 2 parts of cape jasmine, 1 part of fructus amomi；Composition described above,
If 1 part is 100g, 1000 doses of pharmaceutical preparation can be made into.Final drug preparation, such as capsule made of described dose of finger
1000 doses of finger capsule preparations 1000,1000 doses of 1000, tablets of finger of tablet, are 1000 grams of granules for granule.Weight can
To increase or reduce,
But the constant rate of the raw medicinal herbs weight proportion between each composition.
Beneficial effects of the present invention are further illustrated below by way of experimental data：
The preparation method is that obtained by screening, screening process is as follows：
Because the most of active ingredients of square Chinese medicine are water-soluble, therefore extract solvent and be set to water, ginsenoside is slow
One of tired principle active component is resisted in disintegration strenuously, and the total saposins based on ginsenoside is inspection targets, when investigating extraction
Between, the influence of amount of water, extraction time to total saposins, it is determined that optimal extraction process.It the results are shown in Table 1, table 2, table 3.
The orthogonal test gauge outfit L of table 19(34)
Remove and spray the outer medicinal material of powder, take 9 parts of 1/4 recipe quantity (645g) medicinal material, tested by orthogonal test arrangement, filtered,
Filtrate decompression concentrates, and dries, obtains 9 groups of sample dry extracts.According to (total saposins as defined in health food functional component detection method are surveyed
Determine method progress.) total soap amount in medicinal extract is determined, it the results are shown in Table 2.
The orthogonal experiments of table 2
The total saposins variance analysis of table 3
α=0.05
Note：Total saposins %=measures total saposins/(medicinal material amount * dry spuns)
As a result with analysis：Experimental result shows that extraction time has a significant impact to total saposins extraction, extraction time, amount of water
Total saposins extraction is not made significant difference under conditions of design, according to analysis directly perceived, optimum extraction condition is：Get it filled material, add water
Extraction 3 times, extraction 1.5 hours, add 10 times of amount water every time.According to analysis directly perceived, extraction three times increases than extracting total saposins amount twice
Add 7%, 1.5 hours extraction times relatively extracted total saposins amount increase by 1% in 1 hour, and 10 times of amount amount of water are compared with 8 times of amount total soaps of amount of water
Glycosides increase by 6%.Itself 2 times of amount water can be absorbed in itself and is considered from the energy and preliminary experiment medicinal material is saved, and extraction process is modified to：
Remaining Six-element medicinal material, adds water to cook secondary, and for the first time plus the decoctings of 8 times of amounts are boiled 1.5 hours, second plus 6 times of amount decoctings to boil 1 small
When, filtration, merging filtrate.
Checking test
Prescription medicinal material 1.29kg is taken, according to 3 checkings of extraction process progress are determined, part extract dry is taken, calculates checking
It the results are shown in Table 4.
Table 4 extracts checking test result
| Tested number | Yield of extract (%) | Total saposins (%) | Total saposins (g) |
| 1 | 12.87 | 3.15 | 5.23 |
| 2 | 13.17 | 3.19 | 5.40 |
| 3 | 13.33 | 3.24 | 5.57 |
Drying condition screens
Conventional extract dry mode has vacuum drying, microwave drying, constant pressure and dry etc. at present.By medicinal extract obtained by checking test
Mixing, is separately dried, and determines total saponin content, the results are shown in Table 5：
The drying condition of table 5 screens
| Vacuum drying | Microwave drying | Constant pressure and dry | |
| Drying temperature | 60 | 60 | 60 |
| Medicinal extract (g) | 302.7 | 298.5 | 314.2 |
| Dry cream amount (g) | 144.9 | 142.2 | 149.9 |
| Dry cream rate (%) | 12.62 | 12.56 | 12.58 |
| Total saposins (%) | 3.25 | 3.23 | 3.20 |
| Total saposins (g) | 15.87 | 15.70 | 15.57 |
| Drying time (h) | 4 | 1.6 | 6 |
According to conventional auxiliary material, investigate sodium carboxymethyl starch, starch, microcrystalline cellulose (101), calcium monohydrogen phosphate, pregelatinated and form sediment
The auxiliary materials such as powder, low-substituted hydroxypropyl methylcellulose, magnesium stearate, prescription is designed, using broken brittleness, disintegration time limited as target is investigated, sieved
Select prescription.
The prescription screening table of table 6
The effect of in order to preferably control product, the content assaying method of total saposins in side is formulated, specifically, ultraviolet-
Visible spectrophotometry, Detection wavelength are 560 ± 2nm.
Instrument and reagent
Instrument lambda25 spectrophotometry instrument；
Reagent alleviate physical fatigue preparation, ginsenoside re reference substances (Nat'l Pharmaceutical ＆ Biological Products Control Institute provides, for containing
Measure fixed use)；Methanol, ethanol are pure to analyze, and water is ultra-pure water, XAD-2 macroreticular resins, neutral alumina (100-200 mesh).
Sample treatment
1g samples accurately are weighed, are put in 100ml volumetric flasks, adds a small amount of water, ultrasonic 30min, water constant volume is used after cooling, is shaken
It is even, place, Aspirate supernatant 1.0ml carries out column chromatography.
Make chromatography pipe with 10ml syringes, built-in 3cmXAD-2 macroreticular resins, above add 1cm neutral aluminas (100-200
Mesh).Post first is washed with 25ml70% ethanol, then post is washed with 25ml, discards eluent.
It is accurate to add the processed good sample solutions of 1.0ml, post is washed with 25ml, discards eluent.With 25ml70% second
Alcohol elutes ginsenoside, collects eluent in evaporating dish, puts 60 DEG C of water-baths and volatilize, and is used as colour developing with this.
Colour developing
0.2ml5% vanillic aldehyde glacial acetic acid solutions are accurately added in the above-mentioned evaporating dish volatilized, dissolve residue, then are added
0.8ml perchloric acid, colorimetric cylinder is moved into after shaking up, 60 DEG C of heating water bath 10min, is taken out, after ice bath cooling, accurately add glacial acetic acid
5.0ml, using zero pipe as blank, 1cm cuvettes are used after shaking up, absorbance is determined together with standard pipe under 560nm wavelength.
Standard pipe
Accurate ginsenoside Re's standard liquid (2mg/ml) 100ul that draws is put in evaporating dish, and water-bath volatilizes and (is less than 60 DEG C),
By development step, absorbance is determined under 560nm wavelength.
As a result calculate
Total saposins (g/100g)=(ASample/AMark)×CMark×(VSample/mSample)×100/1000/1000
A in formula --- absorbance
M --- sample quality, g
V --- dilution volume, ml
C --- the amount of standard pipe ginsenoside Re, ug
Note：Result of calculation retains two effective digitals
Beneficial effects of the present invention are further illustrated below by way of experimental data：
Test method
1. swimming with a load attached to the body is tested：The sheet lead for 5% body weight that mouse tail is born a heavy burden, is placed in 50cm × 50cm × 40cm ponds
Swimming, depth of water 40cm, 25 DEG C ± 0.5 DEG C of water temperature, per 5, pond mouse, record mouse swimming starts to the death time as mouse
Walking weight load.
2. hepatic glycogen determines：Cervical dislocation puts to death mouse, takes liver, is homogenized, and is operated by glycogen testing cassete specification, surveys
Determine mouse hepatic glycogen content.
3. blood lactase acid determines：After last gives given the test agent 30min, blood 0.1mL is taken from mouse orbit veniplex.Then make
It does not bear a heavy burden stops after the swimming trunk went swimming 10min of 30 DEG C of water temperature, respectively takes blood again after 0,20min after the swimming
0.1mL, operated by full blood lactic testing cassete specification, determine each pipe absorbance, calculated under Serum lactic acid content and blood lactase acid curve
Area.Blood lactase acid TG-AUC=5 × (0min blood lactase acid value+2 × swimming is rested after blood lactase acid value+3 × swimming before swimming
20min blood lactase acid value afterwards).
Experimental agents use medicine prepared by the embodiment of the present invention 1, are referred to as：The benevolence board that converges alleviates physical fatigue preparation
1 converges benevolence board alleviation physical fatigue preparation to the physical influence of mouse
Table 7 is visible, and tested material is high, the walking weight load of middle dose group mouse is considerably longer than blank control group, and difference has
Significant (P<0.05).Show that the tested material has the function that to extend the mice burden swimming time, can determine that remittance benevolence board delays
Physical fatigue preparation, which has, alleviates physical fatigue effect.
The remittance benevolence board of table 7 alleviates influence of the physical fatigue preparation to the mice burden swimming time
Note：Each dosage group is compared with blank control group, * * P ＜ 0.01.
2 remittance benevolence boards alleviate influence of the physical fatigue preparation to hepatic glycogen
From table 8, the hepatic glycogen content of each dosage group mouse of tested material is above blank control group, and difference has statistics
Learn meaning (P<0.01).Show that the tested material has the function that to promote mouse liver glycogen reserves, judge the experimental result positive.
Table 8 converge benevolence board alleviate physical fatigue piece to mouse hepatic glycogen content influence ()
Note：Each dosage group is compared with blank control group, * * P ＜ 0.01.
3 remittance benevolence boards alleviate influence of the physical fatigue preparation to blood lactase acid
From table 9, each dosage group of tested material on mouse swim before blood lactase acid value there are no significant influence (P>0.05) it is, right
Blood lactase acid value is less than blank control group, but no significant difference (P after mouse swimming>0.05)；High, middle dose group can be obvious
The blood lactase acid value after mouse swimming rest 20min is reduced, compared with blank control group, difference has significant (P<0.05).
Tested material is high, middle dose group mouse blood lactase acid TG-AUC is less than blank control group, and difference has significant (P<0.05),
Show that the tested material has the function that lactic acid generates after reducing mouse movement, judges the experimental result positive.
Table 9 converge benevolence board alleviate physical fatigue piece to mouse blood lactase acid value and TG-AUC influence ()
Note：Each dosage group is compared with blank control group, * P ＜ 0.05.
Embodiment：
The present invention is further illustrated with reference to embodiment, but does not indicate that limiting to the claimed invention.
Embodiment 1
The preparation of tablet
Take pilose antler 100g, ginseng 440g, bark of eucommia 700g, Morinda officinalis 640g parts, fruit of Chinese wolfberry 400g, Chinese cassia tree 200g, cape jasmine
200g, fructus amomi 100g.
Pilose antler, part ginseng are ground into 80 mesh, standby；Remaining seven flavor medicine material, add water to cook secondary, first time plus 8 times of amounts
Decocting boil 1.5 hours, second plus 6 times of amount decoctings boil 1 hour, filter, filtrate merges, and adds above-mentioned standby filtrate, decompression
Be condensed into relative density be 1.1~1.2 (60 DEG C) thick paste, 55 DEG C~60 DEG C microwave dryings, crush, add crush after medicinal powder,
Recipe quantity pregelatinized starch, low-substituted hydroxypropyl methylcellulose and low-substituted hydroxypropyl methylcellulose, it is well mixed, adds 75%
Ethanol 40g, pelletized with 24 mesh sieves, 65 DEG C~70 DEG C oven dryings, 20 mesh sieve whole grains, add magnesium stearate, be well mixed, pressure
Piece, film coating, produce.
Embodiment 2
Take 1 part of pilose antler, 4.4 parts of ginseng, 7 parts of the bark of eucommia, 6.4 parts of Morinda officinalis, 4 parts of the fruit of Chinese wolfberry, 2 parts of Chinese cassia tree, 2 parts of cape jasmine, sand
1 part of benevolence.
Wherein 1 part is 100g, pharmaceutically active substance is extracted according to the method for embodiment 1, according to galenic pharmacy routine techniques system
It is standby into capsule.
Embodiment 3
Take 1 part of pilose antler, 4.4 parts of ginseng, 7 parts of the bark of eucommia, 6.4 parts of Morinda officinalis, 4 parts of the fruit of Chinese wolfberry, 2 parts of Chinese cassia tree, 2 parts of cape jasmine, sand
1 part of benevolence.
Wherein 1 part is 100g, pharmaceutically active substance is extracted according to the method for embodiment 1, according to galenic pharmacy routine techniques system
It is standby into granule.
Embodiment 4
Take 1 part of pilose antler, 4.4 parts of ginseng, 7 parts of the bark of eucommia, 6.4 parts of Morinda officinalis, 4 parts of the fruit of Chinese wolfberry, 2 parts of Chinese cassia tree, 2 parts of cape jasmine, sand
1 part of benevolence.
Wherein 1 part is 100g, pharmaceutically active substance is extracted according to the method for embodiment 1, according to galenic pharmacy routine techniques system
It is standby into oral solutions.
Embodiment 5
Pilose antler, part ginseng are ground into 80 mesh, standby；Remaining seven flavor medicine material, add water to cook secondary, first time plus 8 times of amounts
Decocting boil 1.5 hours, second plus 6 times of amount decoctings boil 1 hour, filter, filtrate merges, and adds above-mentioned standby filtrate, decompression
Be condensed into relative density be 1.20~1.35 (60 DEG C) thick paste, 55 DEG C~60 DEG C microwave dryings, crush, add standby medicinal powder,
Recipe quantity pregelatinized starch, starch and low-substituted hydroxypropyl methylcellulose, it is well mixed, 75% ethanol 40g is added, with 24 mesh sieves
Granulation, 65 DEG C~70 DEG C oven dryings, 20 mesh sieve whole grains, magnesium stearate is added, be well mixed, tabletting, film coating, produce.
Claims (6)
- A kind of 1. Chinese medicine composition for alleviating physical fatigue, it is characterised in that：Prepared by the Chinese medicine material medicine of following weight proportion Form, pilose antler 0.5-2 parts, ginseng 2-6 parts, bark of eucommia 3-10 parts, Morinda officinalis 3-8 parts, fruit of Chinese wolfberry 2-6 parts, Chinese cassia tree 1-3 parts, cape jasmine 1-3 parts, fructus amomi 0.5-2 parts.
- 2. the Chinese medicine composition described in claim 1, it is characterised in that：By the Chinese medicine material medicine preparation of following weight proportion Into pilose antler 1-1.5 parts, ginseng 4-5 parts, bark of eucommia 6-7 parts, Morinda officinalis 6-7 parts, fruit of Chinese wolfberry 4-5 parts, Chinese cassia tree 1-2 parts, cape jasmine 1-2 Part, fructus amomi 1-1.5 parts.
- 3. the Chinese medicine composition described in claim 1 or 2, it is characterised in that the pilose antler, ginseng, the bark of eucommia, Morinda officinalis, matrimony vine Son, Chinese cassia tree, cape jasmine, fructus amomi are completely or partially fed intake with its extract.
- 4. according to any Chinese medicine compositions of claim 1-3, it is characterised in that any agent is made with conventional method Type.
- 5. according to any described Chinese medicine compositions of claim 1-3, be made tablet, capsule, oral liquid, granule, pill, Powder, sublimed preparation, supensoid agent, pulvis, solution, suppository, paste, pill or patch.
- 6. application of the Chinese medicine composition in the medicine or health products of alleviating physical fatigue is prepared described in claim 1.
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Address after: 330043 No. 1266 Huiren Avenue, Xiaolan Economic and Technological Development Zone, Nanchang City, Jiangxi Province Co-patentee after: Shanghai Zhongchuang Medicine Science Co., Ltd. Patentee after: Jiangxi Huiren pharmaceutical Limited by Share Ltd Address before: 330052 1189 Yingbin Avenue, Nanchang, Jiangxi Co-patentee before: Shanghai Zhongchuang Medicine Science Co., Ltd. Patentee before: Huiren Pharmaceutical Co., Ltd. Jiangxi |