CN104706855A - Preparation methods and application of Jichuan decoction traditional Chinese medicine extract - Google Patents
Preparation methods and application of Jichuan decoction traditional Chinese medicine extract Download PDFInfo
- Publication number
- CN104706855A CN104706855A CN201510066582.8A CN201510066582A CN104706855A CN 104706855 A CN104706855 A CN 104706855A CN 201510066582 A CN201510066582 A CN 201510066582A CN 104706855 A CN104706855 A CN 104706855A
- Authority
- CN
- China
- Prior art keywords
- raw material
- chinese medicine
- preparation
- medicine extract
- angelicae sinensis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The invention provides preparation methods and an application of a Jichuan decoction traditional Chinese medicine extract. The invention provides five preparation methods: 1) a water extraction method; 2) an alcohol extraction method; 3) a water extraction and alcohol precipitation method; 4) a method combining water extraction with macroporous resin purification; and 5) a method combining alcohol extraction with macroporous resin purification. Based on the Jichuan decoction traditional Chinese medicine extract, a tea bag and an oral preparation of the Jichuan decoction traditional Chinese medicine extract are provided. The prepared products are detected, a conclusion that the content of effective components of the Jichuan decoction traditional Chinese medicine extract is high is proved. The invention also provides the application of the Jichuan decoction traditional Chinese medicine extract in preparation of drugs or health products for treatment of elderly functional constipation, postpartum blood deficiency constipation and weight loss.
Description
Technical field
The present invention relates to preparation method and the application thereof of jichuan decoction side's Chinese medicine extract, belong to technical field of Chinese medicine.
Background technology
The traditional Chinese medical science is thought, although the constipation cause of disease is complicated, nothing more than cold, hot, empty, real, its sick position is at large intestine, closely related with lung, spleen, the dirty function of kidney three, and common with deficient syndrome, especially decline based on deficiency of vital energy sun, have cloud in Jing-Yue Complete Works: " part of the body cavity below the umbilicus, housing the bladder, kidneys and bowels yang deficiency; then yang-energy is not all right, yang-energy is not all right, then can not transmit; and the moon coagulate under, this yang deficiency and constipation of YIN type are also." in addition, gerontal patient's body fluid dries up, blood virtual loss is also constipation major reason.
Jichuan decoction comes from Jing-Yue Complete Works, full side with the kidney warming benefit essence, loosening bowel to relieve constipation for method, wherein Herba Cistanches, Radix Achyranthis Bidentatae warming and recuperating the kidney-YANG, benefiting essence-blood, loosening bowel to relieve constipation; Radix Achyranthis Bidentatae replenishing kidney, strengthening waist, property is apt to descending; Chinese Angelica blood replonishing and blood, loosening bowel to relieve constipation; The wide intestinal therapeutic method to keep the adverse QI flowing downwards of Fructus Aurantii and help relieving constipation; Be equipped with Rhizoma Cimicifugae sending up the lucid YANG, clear sun rises then spontaneous descending of turbid YIN.Full side is based on tonification, and invigorating middle warmer is held concurrently logical, and make healthy energy sufficient, foul smell leaks and constipation must be separated.
At present, Therapeutic Method in doctor trained in Western medicine, mainly contains and to adopt catharsis agent as magnesium sulfate, sodium sulfate, glycerol/sodium chloride (glycerin), glycone etc.But these medicines have more side effect and untoward reaction, as the saline laxatives such as magnesium sulfate are comparatively strong to intestinal stimulation, prolonged application can cause patient to the dependency of medicine, and in addition, prolonged application also can cause melanosis coli.And the wholistic therapy of Chinese medicine to this disease has advantage, be still most important Therapeutic Method.Classical Chinese medicine tradition prescription " jichuan decoction " is the representative of such disease for the treatment of.
" jichuan decoction " side as one well treat Senile Functional Constipation, puerperal blood deficiency and constipation and the prescription of antiobesity action, but its applicating and exploitation does not have market finished product to promote, only be limited to clinician at present to use the decoction of prescription, reason is its extract preparation just decoction, is restricted on its further application.
The mechanism of fat-reducing generally has loosening bowel to relieve constipation, increases satiety, appetite-suppressing, affects fat absorption and metabolism etc.Be easy to cause obesity when people's constipation, and " jichuan decoction " can stimulate intestinal wall effectively, strengthen small intestinal peristalsis, effective maintenance intestinal tract cleaning, the effect of loosening bowel to relieve constipation can be played, discharge vivotoxin is had very great help, so " jichuan decoction " square Chinese medicine extract can play the effect of fat-reducing with keeping the skin wet.
" a kind of Ji Chuan Jian oral preparation and preparation method thereof " patent (patent No. is CN101284051A) of Zhang Wenfang application, relate generally to a kind of Ji Chuan Jian oral preparation and preparation method thereof curing mainly constipation card of suffering from a deficiency of the kidney, extracting method is water extraction, its abstract methods is add the water of 6 ~ 20 times, soak 2 ~ 12 hours, heating extraction 2 ~ 3 times, is prepared into different dosage form by extract.
" a kind of jichuan decoction dosage form preparation technology and production method thereof " patent (patent No. is CN 102283955A) of Yang Hongshu application, relate generally to a kind of jichuan decoction and integrate novel preparation technology and production method thereof, its production process comprises: prepared by newborn even, the nanoparticle of supersonic jet mill, alcohol water extraction, ultrasonic grinding extraction, water boiling concentration, supersonic speed spraying dry, nano-milled, high pressure.
The patent (patent No. is CN 102430014A) of " a kind of preparation method of jichuan decoction particulate oral preparation " of the application such as Li Wenjun, relate generally to a kind of preparation method of oral formulations, extracting method is water extraction, its abstract methods is add the water of 12 times, soak 0.5 ~ 1 hour, decoct 2 times, each 1 ~ 2 hour, simmer down to 1.08 ~ 1.12g/cm
3clear paste, adopt spraying dry and dry granulation.
Wretched insufficiency is there is in above patent or prior art,
1, prescribed dose correctness is relevant with day dose, the dose lonvestion that current open source literature is reported all has certain mistake: " jichuan decoction " square Chinese medicine extract is all with Radix Angelicae Sinensis, Radix Achyranthis Bidentatae, Herba Cistanches, Rhizoma Alismatis, Rhizoma Cimicifugae, Fructus Aurantii is raw material, textual research " jichuan decoction " prescription derives from the Jing-Yue Complete Works that Ming Dynasty's Zhang Jingyue is write, according to known in " Chinese dynasties weights and measures unit value table and explanation " literary composition that Qiu Long writes, 37.3g was equaled the Ming Dynasty 1 liang, be equivalent to 1 money and equal 3.73g, can obtain every day prescription consumption by this metering conversion is Radix Angelicae Sinensis five money [18.7g], Radix Achyranthis Bidentatae two money [7.5g], desertliving cistanche wine washes away salty, three money [11.2g], Rhizoma Alismatis one money half [5.6g], Rhizoma Cimicifugae five points [1.9g], Fructus Aurantii one money [3.7g], each raw material weight is than being Radix Angelicae Sinensis: Radix Achyranthis Bidentatae: Herba Cistanches: Rhizoma Alismatis: Rhizoma Cimicifugae: Fructus Aurantii=5:2:3:1.5:0.5:1.And the dosage that current open source literature is reported, it is different for all measuring with this every day, therefore, needs to revise, and patent of the present invention, has carried out complete confirmation to formula metering reasonability first, and this foothold embodies novelty and the novelty of this patent.
2, the technological parameter of existing public technology to the concrete method such as water extraction, alcohol extraction in " jichuan decoction " side does not have systematic research and confirmation, and do not obtain pilot scale, large examination process certification, embodies the deficiency of existing patented technology, and patented technology of the present invention solves this problem scientifically and rationally..The research of novelty has been carried out to its water extraction, each technological parameter of alcohol extraction process.Simultaneously, in current open source literature report, water extract-alcohol precipitation, the purification by macroporous resin method of " jichuan decoction " side, still without any report, and traditional water extraction, alcohol extraction may cause Aquatic product thing, the water content of alcohol product is high, and purity is inadequate, the easy moisture absorption of product, needs are further purified, and need new technology to quote.Patent of the present invention has used water extract-alcohol precipitation, purification by macroporous resin method first, and has carried out systematic research to its technological parameter.The extract obtained through the preparation method of purification by macroporous resin is compared with the decocting thing of former prescription, and volume is little, Chinese medicine inherent quality is high, and product moisture absorption reduces, stability increases, and easily makes the various dosage forms of appearance looks elegant.
3, existing public technology does not limit its Product characteristics, embody the deficiency of its technology, therefore be badly in need of wanting new analytical method, distinctive restriction is carried out to its main component, make this Product characteristics more reasonable, science is also the novelty of the characterization of modern Chinese medicine prepared product, science, reasonability and novelty.
Summary of the invention
The object of the invention is to overcome the deficiencies in the prior art part, preparation method and the application thereof of jichuan decoction side's Chinese medicine extract is provided.
The present invention includes following technical scheme:
Technical scheme 1: the water extraction preparation method providing jichuan decoction side's Chinese medicine extract, with Radix Angelicae Sinensis, Radix Achyranthis Bidentatae, Herba Cistanches, Rhizoma Alismatis, Rhizoma Cimicifugae, Fructus Aurantii for raw material, each raw material weight is than being Radix Angelicae Sinensis: Radix Achyranthis Bidentatae: Herba Cistanches: Rhizoma Alismatis: Rhizoma Cimicifugae: Fructus Aurantii=5:2:3:1.5:0.5:1; Preparation method comprises the steps: above-mentioned ingredients to clean, take raw material in proportion, add 13 ~ 16 times of water yields and soak 20-30min, heating extraction 3 times, first time extracts 1h, second time extracts 45min, third time extracts 30min, and each extraction time calculates from after boiling, merge extractive liquid, the concentrated extracting solution obtained is 6 ~ 8 times of described raw material weight to described extracting liquid volume, obtains initial concentration extracting solution; Being dried to relative density is further 1.0 ~ 1.2g/cm
3jichuan decoction side's Chinese medicine extract.
Technical scheme 2: the alcohol extraction preparation method providing jichuan decoction side's Chinese medicine extract, with Radix Angelicae Sinensis, Radix Achyranthis Bidentatae, Herba Cistanches, Rhizoma Alismatis, Rhizoma Cimicifugae, Fructus Aurantii for raw material, each raw material weight is than being Radix Angelicae Sinensis: Radix Achyranthis Bidentatae: Herba Cistanches: Rhizoma Alismatis: Rhizoma Cimicifugae: Fructus Aurantii=5:2:3:1.5:0.5:1; Preparation method comprises the steps: above-mentioned ingredients to clean, take raw material in proportion, add 13 ~ 16 times of 65% ~ 85% soak with ethanol 20-30min, heating and refluxing extraction 2 times, each 1h, merge extractive liquid, the concentrated extracting solution obtained to the volume of described extracting solution is 6 ~ 8 times of described raw material weight, obtains initial concentration extracting solution; Being dried to relative density is further 1.0 ~ 1.2g/cm
3jichuan decoction side's Chinese medicine extract.
Technical scheme 3: the water extract-alcohol precipitation preparation method providing jichuan decoction side's Chinese medicine extract, with Radix Angelicae Sinensis, Radix Achyranthis Bidentatae, Herba Cistanches, Rhizoma Alismatis, Rhizoma Cimicifugae, Fructus Aurantii for raw material, each raw material weight is than being Radix Angelicae Sinensis: Radix Achyranthis Bidentatae: Herba Cistanches: Rhizoma Alismatis: Rhizoma Cimicifugae: Fructus Aurantii=5:2:3:1.5:0.5:1; Preparation method comprises the steps:
1) concentrated solution is prepared: above-mentioned ingredients cleaned, take raw material in proportion, add 13 ~ 16 times of water yields and soak 20-30min, heating extraction 3 times, first time extracts 1h, second time extracts 45min, third time extracts 30min, and each extraction time calculates from after boiling, merge extractive liquid, the concentrated extracting solution obtained is 6 ~ 8 times of described raw material weight to described extracting liquid volume, obtains initial concentration extracting solution;
2) add alcohol settling: add ethanol in proper amount in the initial concentration extracting solution of above-mentioned preparation, make solvent alcohol content be 65 ~ 75%, leave standstill 24 hours, filter, filtrate recycling ethanol, obtains refined liquid, concentrated, is drying to obtain jichuan decoction side's Chinese medicine extract.
Technical scheme 4: the purification by macroporous resin preparation method providing jichuan decoction side's Chinese medicine extract, with Radix Angelicae Sinensis, Radix Achyranthis Bidentatae, Herba Cistanches, Rhizoma Alismatis, Rhizoma Cimicifugae, Fructus Aurantii for raw material, each raw material weight is than being Radix Angelicae Sinensis: Radix Achyranthis Bidentatae: Herba Cistanches: Rhizoma Alismatis: Rhizoma Cimicifugae: Fructus Aurantii=5:2:3:1.5:0.5:1; Preparation method comprises the steps:
1) concentrated solution is prepared: above-mentioned ingredients cleaned, take raw material in proportion, add 13 ~ 16 times of water yields and soak 20-30min, heating extraction 3 times, first time extracts 1h, second time extracts 45min, third time extracts 30min, and each extraction time calculates from after boiling, merge extractive liquid, the concentrated extracting solution obtained is 6 ~ 8 times of described raw material weight to described extracting liquid volume, obtains initial concentration extracting solution;
2) purification by macroporous resin: add respectively in the initial concentration extracting solution that 3 ~ 5 times of volume hot water obtain to upper step, get supernatant; Supernatant is crossed macroporous resin column absorption, abandoned stream fluid; Then wash described adsorption column with the water of 1 ~ 5BV with the flow velocity of 0.5 ~ 2.5BV/ hour, abandon water lotion; Adopt the ethanol of 50 ~ 70% afterwards, elution volume 3 ~ 5BV, with the flow velocity eluting adsorption column of 0.5 ~ 2.5BV/ hour, collect this ethanol elution, concentrated, dry described ethanol elution, obtain jichuan decoction side's Chinese medicine extract.
Technical scheme 5: the purification by macroporous resin preparation method providing jichuan decoction side's Chinese medicine extract, with Radix Angelicae Sinensis, Radix Achyranthis Bidentatae, Herba Cistanches, Rhizoma Alismatis, Rhizoma Cimicifugae, Fructus Aurantii for raw material, each raw material weight is than being Radix Angelicae Sinensis: Radix Achyranthis Bidentatae: Herba Cistanches: Rhizoma Alismatis: Rhizoma Cimicifugae: Fructus Aurantii=5:2:3:1.5:0.5:1; Preparation method comprises the steps:
1) concentrated solution is prepared: according to above-mentioned prescription proportioning, with 65% ~ 85% ethanol for raw material described in solvent extraction.Above-mentioned ingredients is cleaned, take raw material in proportion, add 13 ~ 16 times of 65% ~ 85% soak with ethanol 20-30min, heating and refluxing extraction 2 times, each 1h, merge extractive liquid, the concentrated extracting solution obtained to the volume of described extracting solution is 6 ~ 8 times of described raw material weight, obtains initial concentration extracting solution;
2) purification by macroporous resin: add respectively in the initial concentration extracting solution that 3 ~ 5 times of volume hot water obtain to upper step, get supernatant; Supernatant is crossed macroporous resin column absorption, abandoned stream fluid; Then wash described adsorption column with the water of 1 ~ 5BV with the flow velocity of 0.5 ~ 2.5BV/ hour, abandon water lotion; Adopt the ethanol of 50 ~ 70% afterwards, elution volume 3 ~ 5BV, with the flow velocity eluting adsorption column of 0.5 ~ 2.5BV/ hour, collect this ethanol elution, concentrated, dry described ethanol elution, obtain jichuan decoction side's Chinese medicine extract.
In said method of the present invention, described water or soak with ethanol time are 25min.
Technical scheme 6: the preparation method providing jichuan decoction side's teabag, with Radix Angelicae Sinensis, Radix Achyranthis Bidentatae, Herba Cistanches, Rhizoma Alismatis, Rhizoma Cimicifugae, Fructus Aurantii for raw material, each raw material weight is than being Radix Angelicae Sinensis: Radix Achyranthis Bidentatae: Herba Cistanches: Rhizoma Alismatis: Rhizoma Cimicifugae: Fructus Aurantii=5:2:3:1.5:0.5:1; Preparation method comprises the steps: above-mentioned ingredients to clean, and takes raw material in proportion, is ground into coarse granule, coarse granule is distributed into pouch-packaged, make teabag.
Technical scheme 7: the preparation method providing jichuan decoction side's teabag, with Radix Angelicae Sinensis, Radix Achyranthis Bidentatae, Herba Cistanches, Rhizoma Alismatis, Rhizoma Cimicifugae, Fructus Aurantii for raw material, each raw material weight is than being Radix Angelicae Sinensis: Radix Achyranthis Bidentatae: Herba Cistanches: Rhizoma Alismatis: Rhizoma Cimicifugae: Fructus Aurantii=5:2:3:1.5:0.5:1; Preparation method comprises the steps: above-mentioned ingredients to clean, and takes raw material in proportion, is ground into coarse granule, coarse granule and Flos Lonicerae is dressed up pouch-packaged by the weight fraction of 13:1 ~ 13:5, makes teabag.
Technical scheme 8: in jichuan decoction side's Chinese medicine extract that said method of the present invention is prepared and obtained, by weight, ferulic acid is not less than 0.02% to its amount, Hesperetic acid is not less than 0.036%, β-ecdysterone is not less than 0.011%, echinacoside is not less than 0.01%, Alisol B monoacetate is not less than 0.019%, naringin is not less than 1.4%.The content of mentioned component controls, high performance liquid chromatography (HPLC) is adopted to measure by the content of ferulic acid, Hesperetic acid, β-ecdysterone, echinacoside, Alisol B monoacetate, naringin in the extract prepared, obtain for reference substance, mensuration with ferulic acid, Hesperetic acid, β-ecdysterone, echinacoside, verbascoside, Alisol B monoacetate, naringin, neohesperidin.
Technical scheme 9: the oral solid formulation providing the jichuan decoction side's Chinese medicine extract obtained containing said method preparation, wherein oral solid formulation comprises: tablet, hard capsule, soft capsule, granule, oral liquid; Granule, hard capsule and additive of tablet are selected from the conventional adjuvants such as starch, Icing Sugar, lactose, dextrin, stevioside, pregelatinized Starch, microcrystalline Cellulose, calcium bicarbonate, mannitol, hydroxypropyl emthylcellulose, magnesium stearate, micropowder silica gel, Pulvis Talci, polyethylene glycols; Soft capsule adjuvant is selected from Polyethylene Glycol (Macrogol 2000-6000), Cera Flava, liquid paraffin, vegetable oil, glycerol, gelatin etc.Oral liquid adjuvant selects beta-schardinger dextrin-, sorbitol etc., and wherein tablet specifically refers to common compressed tablet, coated tablet, coated tablet, Film coated tablets.
Technical scheme 10: provide said method preparation and jichuan decoction side's Chinese medicine extract of obtain preparation treatment treat Senile Functional Constipation, puerperal blood deficiency and constipation and slimming medicine or health product in application.Toxicity of the present invention is low simultaneously, has good drug safety.
The present invention possesses following advantage:
1, Chinese medicine extract of the present invention is prepared for raw material with Radix Angelicae Sinensis, Radix Achyranthis Bidentatae, Herba Cistanches, Rhizoma Alismatis, Rhizoma Cimicifugae, Fructus Aurantii.Wherein when the root being classified as a umbelliferous kind of plant Radix Angelicae Sinensis (Angelica sinensis (Oliv.) Diels), Radix Achyranthis Bidentatae is the dry root of amaranthaceous plant Radix Achyranthis Bidentatae (Achyranthes bidentata Bl.), Herba Cistanches is the fleshy stem of the dry zone scale leaf of orobanchaceae plant cistanche (Cistanche deserticola Y.C.Ma), Rhizoma Alismatis is the dry tuber of Notes On Alism At Aceae Rhizoma Alismatis (Alisma orientalis (Sam.) Juzep.), Rhizoma Cimicifugae is ranunculaceae plant C.heracleifolia (Cimicifuga heracleifolia Kom.), the dry rhizome of Cimicifuga Dahurica (Cimicifuga dahurica (Turcz.) Maxim.) or Rhizoma Cimicifugae (Cimicifuga foetida L.), Fructus Aurantii is the dry immature fruit of rutaceae Citrus aurantium Linn. (Citrus aurantium L.) and variety thereof.Can adopt the medical material concocted again crude drug in the preparation as required, it is salty that such as Herba Cistanches needs wine to wash away.The Chinese medicine extract obtained is the mixture that contained various active ingredients of medicinal materials works in coordination with onset, and it is different from activated monomer, his drug effect be also different from simply adding of various active ingredients of medicinal materials drug effect and.
2, " jichuan decoction " of the present invention square Chinese medicine extract is all with Radix Angelicae Sinensis, Radix Achyranthis Bidentatae, Herba Cistanches, Rhizoma Alismatis, Rhizoma Cimicifugae, Fructus Aurantii is raw material, the Jing-Yue Complete Works that Ming Dynasty's Zhang Jingyue writes is derived from through examination pertinent literature known " jichuan decoction " prescription, according to known in " Chinese dynasties weights and measures unit value table and explanation " literary composition that Qiu Long writes, 37.3g was equaled the Ming Dynasty 1 liang, be equivalent to 1 money and equal 3.73g, can obtain every day prescription consumption by this metering conversion is Radix Angelicae Sinensis five money [18.7g], Radix Achyranthis Bidentatae two money [7.5g], desertliving cistanche wine washes away salty, three money [11.2g], Rhizoma Alismatis one money half [5.6g], Rhizoma Cimicifugae five points [1.9g], Fructus Aurantii one money [3.7g], each raw material weight is than being Radix Angelicae Sinensis: Radix Achyranthis Bidentatae: Herba Cistanches: Rhizoma Alismatis: Rhizoma Cimicifugae: Fructus Aurantii=5:2:3:1.5:0.5:1.
3, present invention employs the multiple methods such as water extraction, alcohol extraction, water extract-alcohol precipitation, purification by macroporous resin, fundamentally ensureing the superiority of " jichuan decoction " square Chinese medicine extract, providing safeguard for being prepared into high-quality different dosage form product.The preparation technology of extract provided by the invention can enrichment main active effectively as ferulic acid, Hesperetic acid, β-ecdysterone, echinacoside, Alisol B monoacetate, naringin etc.; Quality control adopts high efficient liquid phase analysis method to carry out assay, simple and easy to control.
4, adopt resin purification method in purification by macroporous resin preparation method provided by the invention, the method is easy, quick, favorable reproducibility; Selected macroporous resin adsorption amount is large, and desorption efficiency is large, can Reusability, reduces process costs; Etoh solvent used is inexpensive nontoxic.The extract obtained through the preparation method of purification by macroporous resin is compared with the decocting thing of former prescription, and volume is little, Chinese medicine inherent quality is high, and product moisture absorption reduces, stability increases, and easily makes the various dosage forms of appearance looks elegant.
5, the jichuan decoction side's Chinese medicine extract prepared by preparation method of the present invention has treatment veteran form constipation and the function such as astriction of deficiency of blood type and Weight-reducing and lipid-lowering.And toxicity is low, there is good drug safety, the safety of the clinical application of this extract of fully having pointed out this technique to prepare.
Therefore, " jichuan decoction " the square Chinese medicine extract described in the present invention is applicable to suitability for industrialized production, has very strong practicality.
Detailed description of the invention
By the essence that the following examples can better understand the present invention, but do not limit the present invention in any way.
Material source: the root of the umbelliferous kind of plant Radix Angelicae Sinensis of angelica (Angelica sinensis (Oliv.) Diels), Radix Achyranthis Bidentatae is the dry root of amaranthaceous plant Radix Achyranthis Bidentatae (Achyranthes bidentata Bl.), Herba Cistanches is the fleshy stem of the dry zone scale leaf of orobanchaceae plant cistanche (Cistanche deserticola Y.C.Ma), Rhizoma Alismatis is the dry tuber of Notes On Alism At Aceae Rhizoma Alismatis (Alisma orientalis (Sam.) Juzep.), Rhizoma Cimicifugae is ranunculaceae plant C.heracleifolia (Cimicifuga heracleifolia Kom.), the dry rhizome of Cimicifuga Dahurica (Cimicifuga dahurica (Turcz.) Maxim.) or Rhizoma Cimicifugae (Cimicifuga foetida L.), Fructus Aurantii is the dry immature fruit of rutaceae Citrus aurantium Linn. (Citrus aurantium L.) and variety thereof.Wherein to need wine to wash away salty for Herba Cistanches, and business is bought and obtained, and other materials is all bought by commercial sources.
Embodiment 1 water extracting method preparation " jichuan decoction " square Chinese medicine extract
Method 1: get Radix Angelicae Sinensis, Radix Achyranthis Bidentatae, Herba Cistanches, Rhizoma Alismatis, Rhizoma Cimicifugae, Fructus Aurantii be raw material, take water as raw material described in solvent extraction, each raw material weight is than being Radix Angelicae Sinensis: Radix Achyranthis Bidentatae: Herba Cistanches: Rhizoma Alismatis: Rhizoma Cimicifugae: Fructus Aurantii
=5:2:3:1.5:0.5:1; Above-mentioned ingredients is cleaned, take Radix Angelicae Sinensis 50 kilograms, Radix Achyranthis Bidentatae 20 kilograms, Herba Cistanches 30 kilograms, Rhizoma Alismatis 15 kilograms, Rhizoma Cimicifugae 5 kilograms, Fructus Aurantii 10 kilograms in proportion, 1.69 tons of water yields soak 20min, heating extraction 3 (first time 1h, second time 45min, third time 30min, each extraction time calculates from after boiling), merge extractive liquid, the concentrated extracting solution obtained is 6 times of described raw material weight to described extracting liquid volume, obtains initial concentration extracting solution; Being dried to relative density is further 1.0g/cm
3" jichuan decoction " square Chinese medicine extract (lot number is 14010106).
Method 2: get Radix Angelicae Sinensis, Radix Achyranthis Bidentatae, Herba Cistanches, Rhizoma Alismatis, Rhizoma Cimicifugae, Fructus Aurantii be raw material, take water as raw material described in solvent extraction, each raw material weight is than being Radix Angelicae Sinensis: Radix Achyranthis Bidentatae: Herba Cistanches: Rhizoma Alismatis: Rhizoma Cimicifugae: Fructus Aurantii=5:2:3:1.5:0.5:1; Above-mentioned ingredients is cleaned, take Radix Angelicae Sinensis 50 kilograms, Radix Achyranthis Bidentatae 20 kilograms, Herba Cistanches 30 kilograms, Rhizoma Alismatis 15 kilograms, Rhizoma Cimicifugae 5 kilograms, Fructus Aurantii 10 kilograms in proportion, 2.08 tons of water yields soak 30min, heating extraction 3 (first time 1h, second time 45min, third time 30min, each extraction time calculates from after boiling), merge extractive liquid, the concentrated extracting solution obtained is 8 times of described raw material weight to described extracting liquid volume, obtains initial concentration extracting solution; Being dried to relative density is further 1.2g/cm
3" jichuan decoction " square Chinese medicine extract (lot number is 14010208).
Method 3: get Radix Angelicae Sinensis, Radix Achyranthis Bidentatae, Herba Cistanches, Rhizoma Alismatis, Rhizoma Cimicifugae, Fructus Aurantii be raw material, take water as raw material described in solvent extraction, each raw material weight is than being Radix Angelicae Sinensis: Radix Achyranthis Bidentatae: Herba Cistanches: Rhizoma Alismatis: Rhizoma Cimicifugae: Fructus Aurantii=5:2:3:1.5:0.5:1; Above-mentioned ingredients is cleaned, take Radix Angelicae Sinensis 50 kilograms, Radix Achyranthis Bidentatae 20 kilograms, Herba Cistanches 30 kilograms, Rhizoma Alismatis 15 kilograms, Rhizoma Cimicifugae 5 kilograms, Fructus Aurantii 10 kilograms in proportion, 1.85 tons of water yields soak 25min, heating extraction 3 (first time 1h, second time 45min, third time 30min, each extraction time calculates from after boiling), merge extractive liquid, the concentrated extracting solution obtained is 7 times of described raw material weight to described extracting liquid volume, obtains initial concentration extracting solution; Being dried to relative density is further 1.1g/cm
3" jichuan decoction " square Chinese medicine extract (lot number is 14010309).
Embodiment 2 alcohol extraction method preparation " jichuan decoction " square Chinese medicine extract
Method 1: get Radix Angelicae Sinensis, Radix Achyranthis Bidentatae, Herba Cistanches, Rhizoma Alismatis, Rhizoma Cimicifugae, Fructus Aurantii be raw material, with 65% ethanol for raw material described in solvent extraction, each raw material weight is than being Radix Angelicae Sinensis: Radix Achyranthis Bidentatae: Herba Cistanches: Rhizoma Alismatis: Rhizoma Cimicifugae: Fructus Aurantii=5:2:3:1.5:0.5:1; Above-mentioned ingredients is cleaned, take Radix Angelicae Sinensis 50 kilograms, Radix Achyranthis Bidentatae 20 kilograms, Herba Cistanches 30 kilograms, Rhizoma Alismatis 15 kilograms, Rhizoma Cimicifugae 5 kilograms, Fructus Aurantii 10 kilograms in proportion, 1.69 ton of 65% soak with ethanol 20min, heating and refluxing extraction 2 times, each 1h (each reflux extracting time calculates from after boiling), extracting solution merges, leave standstill 24 hours, cross leaching supernatant, by crude drug 6 times of supernatant concentration, obtain initial concentration extracting solution; Being dried to relative density is further 1.0g/cm
3" jichuan decoction " square Chinese medicine extract (lot number is 14020423).
Method 2: get Radix Angelicae Sinensis, Radix Achyranthis Bidentatae, Herba Cistanches, Rhizoma Alismatis, Rhizoma Cimicifugae, Fructus Aurantii be raw material, with 85% ethanol for raw material described in solvent extraction, each raw material weight is than being Radix Angelicae Sinensis: Radix Achyranthis Bidentatae: Herba Cistanches: Rhizoma Alismatis: Rhizoma Cimicifugae: Fructus Aurantii=5:2:3:1.5:0.5:1; Above-mentioned ingredients is cleaned, take Radix Angelicae Sinensis 50 kilograms, Radix Achyranthis Bidentatae 20 kilograms, Herba Cistanches 30 kilograms, Rhizoma Alismatis 15 kilograms, Rhizoma Cimicifugae 5 kilograms, Fructus Aurantii 10 kilograms in proportion, 2.08 ton of 85% soak with ethanol 30min, heating and refluxing extraction 2 times, each 1h (each reflux extracting time calculates from after boiling), extracting solution merges, leave standstill 24 hours, cross leaching supernatant, by crude drug 8 times of supernatant concentration, obtain initial concentration extracting solution; Being dried to relative density is further 1.2g/cm
3" jichuan decoction " Chinese medicine alcohol extract (lot number is 14020524).
Method 3: get Radix Angelicae Sinensis, Radix Achyranthis Bidentatae, Herba Cistanches, Rhizoma Alismatis, Rhizoma Cimicifugae, Fructus Aurantii be raw material, with 75% ethanol for raw material described in solvent extraction, each raw material weight is than being Radix Angelicae Sinensis: Radix Achyranthis Bidentatae: Herba Cistanches: Rhizoma Alismatis: Rhizoma Cimicifugae: Fructus Aurantii=5:2:3:1.5:0.5:1; Above-mentioned ingredients is cleaned, take Radix Angelicae Sinensis 50 kilograms, Radix Achyranthis Bidentatae 20 kilograms, Herba Cistanches 30 kilograms, Rhizoma Alismatis 15 kilograms, Rhizoma Cimicifugae 5 kilograms, Fructus Aurantii 10 kilograms in proportion, 1.85 ton of 75% soak with ethanol 25min, heating and refluxing extraction 2 times, each 1h (each reflux extracting time calculates from after boiling), extracting solution merges, leave standstill 24 hours, cross leaching supernatant, by crude drug 7 times of supernatant concentration, obtain initial concentration extracting solution; Being dried to relative density is further 1.1g/cm
3" jichuan decoction " Chinese medicine alcohol extract (lot number is 14020625).
Embodiment 3 water extract-alcohol precipitation method preparation " jichuan decoction " square Chinese medicine extract
Method 1: get Radix Angelicae Sinensis, Radix Achyranthis Bidentatae, Herba Cistanches, Rhizoma Alismatis, Rhizoma Cimicifugae, Fructus Aurantii be raw material, take water as raw material described in solvent extraction, each raw material weight is than being Radix Angelicae Sinensis: Radix Achyranthis Bidentatae: Herba Cistanches: Rhizoma Alismatis: Rhizoma Cimicifugae: Fructus Aurantii=5:2:3:1.5:0.5:1; Above-mentioned ingredients is cleaned, take Radix Angelicae Sinensis 50 kilograms, Radix Achyranthis Bidentatae 20 kilograms, Herba Cistanches 30 kilograms, Rhizoma Alismatis 15 kilograms, Rhizoma Cimicifugae 5 kilograms, Fructus Aurantii 10 kilograms in proportion, add 1.69 tons: the ton water yield soaks 20min, heating extraction 3 (first time 1h, second time 45min, third time 30min, each extraction time calculates from after boiling), merge extractive liquid, the concentrated extracting solution obtained is 6 times of described raw material weight to described extracting liquid volume, obtains initial concentration extracting solution; Slowly add ethanol in proper amount while stirring, make solvent alcohol content be 65%, leave standstill 24 hours, filter, filtrate recycling ethanol, obtains refined liquid, concentrated, dry acquisition " jichuan decoction " square Chinese medicine extract (lot number is 14030518).
Method 2: get Radix Angelicae Sinensis, Radix Achyranthis Bidentatae, Herba Cistanches, Rhizoma Alismatis, Rhizoma Cimicifugae, Fructus Aurantii be raw material, take water as raw material described in solvent extraction, each raw material weight is than being Radix Angelicae Sinensis: Radix Achyranthis Bidentatae: Herba Cistanches: Rhizoma Alismatis: Rhizoma Cimicifugae: Fructus Aurantii=5:2:3:1.5:0.5:1; Above-mentioned ingredients is cleaned, take Radix Angelicae Sinensis 50 kilograms, Radix Achyranthis Bidentatae 20 kilograms, Herba Cistanches 30 kilograms, Rhizoma Alismatis 15 kilograms, Rhizoma Cimicifugae 5 kilograms, Fructus Aurantii 10 kilograms in proportion, add 2.08 tons of water yields and soak 30min, heating extraction 3 (first time 1h, second time 45min, third time 30min, each extraction time calculates from after boiling), merge extractive liquid, the concentrated extracting solution obtained is 8 times of described raw material weight to described extracting liquid volume, obtains initial concentration extracting solution; Slowly add ethanol in proper amount while stirring, make solvent alcohol content be 75%, leave standstill 24 hours, filter, filtrate recycling ethanol, obtains refined liquid, concentrated, dry acquisition " jichuan decoction " square Chinese medicine extract (lot number is 14030620).
Method 3: get Radix Angelicae Sinensis, Radix Achyranthis Bidentatae, Herba Cistanches, Rhizoma Alismatis, Rhizoma Cimicifugae, Fructus Aurantii be raw material, take water as raw material described in solvent extraction, each raw material weight is than being Radix Angelicae Sinensis: Radix Achyranthis Bidentatae: Herba Cistanches: Rhizoma Alismatis: Rhizoma Cimicifugae: Fructus Aurantii=5:2:3:1.5:0.5:1; Above-mentioned ingredients is cleaned, take Radix Angelicae Sinensis 50 kilograms, Radix Achyranthis Bidentatae 20 kilograms, Herba Cistanches 30 kilograms, Rhizoma Alismatis 15 kilograms, Rhizoma Cimicifugae 5 kilograms, Fructus Aurantii 10 kilograms in proportion, add 1.85 tons of water yields and soak 25min, heating extraction 3 (first time 1h, second time 45min, third time 30min, each extraction time calculates from after boiling), merge extractive liquid, the concentrated extracting solution obtained is 7 times of described raw material weight to described extracting liquid volume, obtains initial concentration extracting solution; Slowly add ethanol in proper amount while stirring, make solvent alcohol content be 70%, leave standstill 24 hours, filter, filtrate recycling ethanol, obtains refined liquid, concentrated, dry acquisition " jichuan decoction " square Chinese medicine extract (lot number is 14030722).
Embodiment 4 macroporous resin method one prepares " jichuan decoction " square Chinese medicine extract
Method 1: get Radix Angelicae Sinensis, Radix Achyranthis Bidentatae, Herba Cistanches, Rhizoma Alismatis, Rhizoma Cimicifugae, Fructus Aurantii be raw material, take water as raw material described in solvent extraction, each raw material weight is than being Radix Angelicae Sinensis: Radix Achyranthis Bidentatae: Herba Cistanches: Rhizoma Alismatis: Rhizoma Cimicifugae: Fructus Aurantii=5:2:3:1.5:0.5:1; Above-mentioned ingredients is cleaned, take Radix Angelicae Sinensis 50 kilograms, Radix Achyranthis Bidentatae 20 kilograms, Herba Cistanches 30 kilograms, Rhizoma Alismatis 15 kilograms, Rhizoma Cimicifugae 5 kilograms, Fructus Aurantii 10 kilograms in proportion, 1.69 tons of water yields soak 20min, heating extraction 3 (first time 1h, second time 45min, third time 30min, each extraction time calculates from after boiling), merge extractive liquid, the concentrated extracting solution obtained is 6 times of described raw material weight to described extracting liquid volume, obtains initial concentration extracting solution; Initial concentration extracting solution is added 3 times of hot water dilutions, add appropriate Chinese medicine clarifier chitosan, centrifugal, go precipitation, get supernatant, be splined on D101 macroporous resin column and (before loading, use acid respectively, alkali, water, alcohol carries out pre-treatment to resin column), first use 1BV pure water rinsing after loading, aqueous solution reject, use 50% ethanol elution of 3BV instead, eluent flow rate is 0.5BV/h, collects this ethanol elution.Continue decompression and solvent recovery, collect extractum, dry acquisition " jichuan decoction " square Chinese medicine extract (lot number is 14040817).
Method 2: get Radix Angelicae Sinensis, Radix Achyranthis Bidentatae, Herba Cistanches, Rhizoma Alismatis, Rhizoma Cimicifugae, Fructus Aurantii be raw material, take water as raw material described in solvent extraction, each raw material weight is than being Radix Angelicae Sinensis: Radix Achyranthis Bidentatae: Herba Cistanches: Rhizoma Alismatis: Rhizoma Cimicifugae: Fructus Aurantii=5:2:3:1.5:0.5:1; Above-mentioned ingredients is cleaned, take Radix Angelicae Sinensis 50 kilograms, Radix Achyranthis Bidentatae 20 kilograms, Herba Cistanches 30 kilograms, Rhizoma Alismatis 15 kilograms, Rhizoma Cimicifugae 5 kilograms, Fructus Aurantii 10 kilograms in proportion, 2.08 tons of water yields soak 30min, heating extraction 3 (first time 1h, second time 45min, third time 30min, each extraction time calculates from after boiling), merge extractive liquid, the concentrated extracting solution obtained is 8 times of described raw material weight to described extracting liquid volume, obtains initial concentration extracting solution; Initial concentration extracting solution is added 5 times of hot water dilutions, add appropriate Chinese medicine clarifier chitosan, centrifugal, go precipitation, get supernatant, be splined on D101 macroporous resin column and (before loading, use acid respectively, alkali, water, alcohol carries out pre-treatment to resin column), first use 5BV pure water rinsing after loading, aqueous solution reject, use 70% ethanol elution of 5BV instead, eluent flow rate is 2.5BV/h, collects this ethanol elution.Continue decompression and solvent recovery, collect extractum, dry acquisition " jichuan decoction " square Chinese medicine extract (lot number is 14040927).
Method 3: get Radix Angelicae Sinensis, Radix Achyranthis Bidentatae, Herba Cistanches, Rhizoma Alismatis, Rhizoma Cimicifugae, Fructus Aurantii be raw material, take water as raw material described in solvent extraction, each raw material weight is than being Radix Angelicae Sinensis: Radix Achyranthis Bidentatae: Herba Cistanches: Rhizoma Alismatis: Rhizoma Cimicifugae: Fructus Aurantii=5:2:3:1.5:0.5:1; Above-mentioned ingredients is cleaned, take Radix Angelicae Sinensis 50 kilograms, Radix Achyranthis Bidentatae 20 kilograms, Herba Cistanches 30 kilograms, Rhizoma Alismatis 15 kilograms, Rhizoma Cimicifugae 5 kilograms, Fructus Aurantii 10 kilograms in proportion, 1.85 tons of water yields soak 25min, heating extraction 3 (first time 1h, second time 45min, third time 30min, each extraction time calculates from after boiling), merge extractive liquid, the concentrated extracting solution obtained is 7 times of described raw material weight to described extracting liquid volume, obtains initial concentration extracting solution; Initial concentration extracting solution is added 4 times of hot water dilutions, add appropriate Chinese medicine clarifier chitosan, centrifugal, go precipitation, get supernatant, be splined on D101 macroporous resin column and (before loading, use acid respectively, alkali, water, alcohol carries out pre-treatment to resin column), first use 2.5BV pure water rinsing after loading, aqueous solution reject, use 60% ethanol elution of 4BV instead, eluent flow rate is 1.5BV/h, collects this ethanol elution.Continue decompression and solvent recovery, collect extractum, dry acquisition " jichuan decoction " square Chinese medicine extract (lot number is 14041021).
Embodiment 5 macroporous resin method two prepares " jichuan decoction " square Chinese medicine extract
Method 1: get Radix Angelicae Sinensis, Radix Achyranthis Bidentatae, Herba Cistanches, Rhizoma Alismatis, Rhizoma Cimicifugae, Fructus Aurantii be raw material, with 65% ethanol for raw material described in solvent extraction, each raw material weight is than being Radix Angelicae Sinensis: Radix Achyranthis Bidentatae: Herba Cistanches: Rhizoma Alismatis: Rhizoma Cimicifugae: Fructus Aurantii=5:2:3:1.5:0.5:1; Above-mentioned ingredients is cleaned, take Radix Angelicae Sinensis 50 kilograms, Radix Achyranthis Bidentatae 20 kilograms, Herba Cistanches 30 kilograms, Rhizoma Alismatis 15 kilograms, Rhizoma Cimicifugae 5 kilograms, Fructus Aurantii 10 kilograms in proportion, 1.69 ton of 65% soak with ethanol 20min, heating and refluxing extraction 2 times, each 1h (each reflux extracting time calculates from after boiling), extracting solution merges, leave standstill 24 hours, cross leaching supernatant, by crude drug 6 times of supernatant concentration, obtain initial concentration extracting solution; Concentrated extracting solution is added 3 times of hot water dilutions, add appropriate Chinese medicine clarifier chitosan, centrifugal, go precipitation, get supernatant, be splined on D101 macroporous resin column and (before loading, use acid respectively, alkali, water, alcohol carries out pre-treatment to resin column), first use 5BV water (regulating PH to be 5 with 1% hydrochloric acid) to wash described adsorption column after loading, abandon water lotion; Use neutral 1BV aqueous solution eluting again, aqueous solution reject, use 50% ethanol elution of 3BV instead, elution flow rate is 0.5BV/h, collects this ethanol elution.Continue decompression and solvent recovery, collect extractum, dry acquisition " jichuan decoction " square Chinese medicine extract (lot number is 14051103).
Method 2: get Radix Angelicae Sinensis, Radix Achyranthis Bidentatae, Herba Cistanches, Rhizoma Alismatis, Rhizoma Cimicifugae, Fructus Aurantii be raw material, with 85% ethanol for raw material described in solvent extraction, each raw material weight is than being Radix Angelicae Sinensis: Radix Achyranthis Bidentatae: Herba Cistanches: Rhizoma Alismatis: Rhizoma Cimicifugae: Fructus Aurantii=5:2:3:1.5:0.5:1; Above-mentioned ingredients is cleaned, take Radix Angelicae Sinensis 50 kilograms, Radix Achyranthis Bidentatae 20 kilograms, Herba Cistanches 30 kilograms, Rhizoma Alismatis 15 kilograms, Rhizoma Cimicifugae 5 kilograms, Fructus Aurantii 10 kilograms in proportion, 2.08 ton of 85% soak with ethanol 30min, heating and refluxing extraction 2 times, each 1h (each reflux extracting time calculates from after boiling), extracting solution merges, leave standstill 24 hours, cross leaching supernatant, by crude drug 8 times of supernatant concentration, obtain initial concentration extracting solution; Concentrated extracting solution is added 5 times of hot water dilutions, add appropriate Chinese medicine clarifier chitosan, centrifugal, go precipitation, get supernatant, be splined on D101 macroporous resin column and (before loading, use acid respectively, alkali, water, alcohol carries out pre-treatment to resin column), first use 5BV water (regulating PH to be 6 with 1% hydrochloric acid) to wash described adsorption column after loading, abandon water lotion; Use neutral 5BV aqueous solution eluting again, aqueous solution reject, use 70% ethanol elution washing 5BV instead, elution flow rate is 2.5BV/h, collects this ethanol elution.Continue decompression and solvent recovery, collect extractum, dry acquisition " jichuan decoction " square Chinese medicine extract (lot number is 14051205).
Method 3: get Radix Angelicae Sinensis, Radix Achyranthis Bidentatae, Herba Cistanches, Rhizoma Alismatis, Rhizoma Cimicifugae, Fructus Aurantii be raw material, with 75% ethanol for raw material described in solvent extraction, each raw material weight is than being Radix Angelicae Sinensis: Radix Achyranthis Bidentatae: Herba Cistanches: Rhizoma Alismatis: Rhizoma Cimicifugae: Fructus Aurantii=5:2:3:1.5:0.5:1; Above-mentioned ingredients is cleaned, take Radix Angelicae Sinensis 50 kilograms, Radix Achyranthis Bidentatae 20 kilograms, Herba Cistanches 30 kilograms, Rhizoma Alismatis 15 kilograms, Rhizoma Cimicifugae 5 kilograms, Fructus Aurantii 10 kilograms in proportion, 1.85 ton of 75% soak with ethanol 25min, heating and refluxing extraction 2 times, each 1h (each reflux extracting time calculates from after boiling), extracting solution merges, leave standstill 24 hours, cross leaching supernatant, by crude drug 7 times of supernatant concentration, obtain initial concentration extracting solution; Concentrated extracting solution is added 5 times of hot water dilutions, add appropriate Chinese medicine clarifier chitosan, centrifugal, go precipitation, get supernatant, be splined on D101 macroporous resin column and (before loading, use acid respectively, alkali, water, alcohol carries out pre-treatment to resin column), first use 5BV water (regulating PH to be 5.5 with 1% hydrochloric acid) to wash described adsorption column after loading, abandon water lotion; Use neutral 3BV aqueous solution eluting again, aqueous solution reject, use 70% ethanol elution washing 4BV instead, elution flow rate is 1.5BV/h, collects this ethanol elution.Continue decompression and solvent recovery, collect extractum, dry acquisition " jichuan decoction " square Chinese medicine extract (lot number is 14051228).
The oral formulations preparation method of embodiment 6 " jichuan decoction " square Chinese medicine extract
1) preparation of " jichuan decoction " square Chinese medicine extract granule
Prescription: (jichuan decoction extract is taken from " jichuan decoction " square Chinese medicine extract of embodiment 1, embodiment 2, embodiment 3, embodiment 4, embodiment 5 respectively)
Jichuan decoction extract: 500.0g
Starch: 200.0g
Calcium hydrogen phosphate: 150.0g
Dextrin: 100.0g
Lactose: 50.0g
Take recipe quantity jichuan decoction extract, add the adjuvant of recipe quantity, add 60% ethanol soft material, cross 20 orders and granulate, 50 ~ 60 DEG C of forced air dryings (moisture control is 5% ~ 8%), cross 16 mesh sieve granulate, obtain jichuan decoction granule.
2) preparation of " jichuan decoction " square Chinese medicine extract coated tablet
Prescription: (jichuan decoction extract is taken from " jichuan decoction " square Chinese medicine extract of embodiment 1, embodiment 2, embodiment 3, embodiment 4, embodiment 5 respectively)
Jichuan decoction extract: 500.0g
Pregelatinized Starch: 200.0g
Microcrystalline Cellulose: 230.0g
Stevioside: 25.0g
Sucrose: 25.0g
Magnesium stearate: 10.0g
Micropowder silica gel: 10.0g
Coating materials: color TM gastric solubleness coating materials (in right amount) of promise
Take the jichuan decoction extract of recipe quantity, add the adjuvant (magnesium stearate, micropowder silica gel additional) of recipe quantity, add 60% ethanol soft material, cross 20 orders to granulate, 50 ~ 60 DEG C of forced air dryings (moisture control is 5% ~ 8%), cross 16 mesh sieve granulate, mix homogeneously with additional adjuvant magnesium stearate, micropowder silica gel, tabletting, coating, obtains JICHUAN Pan-Fried Dumplings garment piece.
3) preparation of " jichuan decoction " square Chinese medicine extract capsule
Prescription: (jichuan decoction extract is taken from " jichuan decoction " square Chinese medicine extract of embodiment 1, embodiment 2, embodiment 3, embodiment 4, embodiment 5 respectively)
Jichuan decoction extract: 500.0g
Hypromellose: 40.0g (the additional half Inner of half adds)
Pregelatinized Starch: 190.0g
Microcrystalline Cellulose: 200.0g
Lactose: 25.0g
Icing Sugar: 25.0g
Magnesium stearate: 10.0g
Pulvis Talci: 10.0g
Take recipe quantity jichuan decoction extract, add the adjuvant (magnesium stearate, Pulvis Talci are additional) of recipe quantity, add 60% ethanol soft material, cross 20 orders to granulate, 50 ~ 60 DEG C of forced air dryings (moisture control is 5% ~ 8%), cross 16 mesh sieve granulate, mix homogeneously with additional adjuvant magnesium stearate, micropowder silica gel, fill with filling machine, obtain jichuan decoction capsule.
4) preparation of " jichuan decoction " square Chinese medicine extract soft capsule
Prescription: (jichuan decoction extract is taken from " jichuan decoction " square Chinese medicine extract of embodiment 1, embodiment 2, embodiment 3, embodiment 4, embodiment 5 respectively)
Jichuan decoction extract: 500.0g
Vegetable oil: 140.0g
Sorbitol: 100.0g
Glycerol: 50.0g
Liquid paraffin: 60.0g
Gelatin: 150.0g
The vegetable oil, the sorbitol that take recipe quantity jichuan decoction extract and recipe quantity are mixed homogeneously, gelatin and glycerol are distinguished splendid attire in hopper, two liquid are sprayed with friction speed by the double-deck shower nozzle of concentric tube-shaped, make a certain amount of glue by after a certain amount of medicinal liquid parcel, in the another kind of liquid coolant (liquid paraffin) of not dissolving each other mutually of instillation, because surface tension effects becomes spherical after glue contact cooling liquid, and solidify gradually and form soft capsule.
5) preparation of " jichuan decoction " square Chinese medicine extract oral liquid
Prescription: (jichuan decoction extract is taken from " jichuan decoction " square Chinese medicine extract of embodiment 1, embodiment 2, embodiment 3, embodiment 4, embodiment 5 respectively)
Jichuan decoction extract: 500.0g
Beta-schardinger dextrin-: 140.0g
Sucrose: 100.0g
Distilled water 260.0g
Take recipe quantity jichuan decoction extract, add the adjuvant of recipe quantity, adding distil water dissolves, and stirs, obtains jichuan decoction extract oral liquid.
6) preparation of " jichuan decoction " square teabag
Prescription one:
Radix Angelicae Sinensis: 500.0g
Radix Achyranthis Bidentatae: 200.0g
Herba Cistanches: 300.0g
Rhizoma Alismatis: 150g
Rhizoma Cimicifugae: 50g
Fructus Aurantii: 100g
Above-mentioned ingredients is cleaned, takes raw material by prescription, be ground into coarse granule, coarse granule is distributed into 100 pouches, makes teabag.
Prescription two:
Radix Angelicae Sinensis: 500.0g
Radix Achyranthis Bidentatae: 200.0g
Herba Cistanches: 300.0g
Rhizoma Alismatis: 150g
Rhizoma Cimicifugae: 50g
Fructus Aurantii: 100g
Flos Lonicerae: 100g
Above-mentioned ingredients is cleaned, takes raw material (Radix Angelicae Sinensis, Radix Achyranthis Bidentatae, Herba Cistanches, Rhizoma Alismatis, Rhizoma Cimicifugae, Fructus Aurantii) by prescription, be ground into coarse granule, coarse granule and Flos Lonicerae are dressed up 100 pouches by the weight fraction of 13:1, makes teabag.
Prescription three:
Radix Angelicae Sinensis: 500.0g
Radix Achyranthis Bidentatae: 200.0g
Herba Cistanches: 300.0g
Rhizoma Alismatis: 150g
Rhizoma Cimicifugae: 50g
Fructus Aurantii: 100g
Flos Lonicerae: 300g
Above-mentioned ingredients is cleaned, takes raw material (Radix Angelicae Sinensis, Radix Achyranthis Bidentatae, Herba Cistanches, Rhizoma Alismatis, Rhizoma Cimicifugae, Fructus Aurantii) by prescription, be ground into coarse granule, coarse granule and Flos Lonicerae are dressed up 100 pouches by the weight fraction of 13:3, makes teabag.
Prescription four:
Radix Angelicae Sinensis: 500.0g
Radix Achyranthis Bidentatae: 200.0g
Herba Cistanches: 300.0g
Rhizoma Alismatis: 150g
Rhizoma Cimicifugae: 50g
Fructus Aurantii: 100g
Flos Lonicerae: 500g
Above-mentioned ingredients is cleaned, takes raw material (Radix Angelicae Sinensis, Radix Achyranthis Bidentatae, Herba Cistanches, Rhizoma Alismatis, Rhizoma Cimicifugae, Fructus Aurantii) by prescription, be ground into coarse granule, coarse granule and Flos Lonicerae are dressed up 100 pouches by the weight fraction of 13:5, makes teabag.
The determination of bioactive constituent of embodiment 7 " jichuan decoction " formula extraction
1. chromatographic condition
Agilent1260 high performance liquid chromatograph, UV-detector (UV) is filler with octadecylsilane chemically bonded silica, C18 chromatographic column (4.6mm × 250mm, 5 μm), ferulic acid (National Institute for Food and Drugs Control, lot number: 110773-20101), Hesperetic acid (National Institute for Food and Drugs Control, lot number: 111698-20110) inspection wavelength: 316nm, mobile phase: acetonitrile-0.1% phosphoric acid solution (12:88-20:80 gradient elution), β-ecdysterone (Nat'l Pharmaceutical & Biological Products Control Institute, lot number: 110709-201206), echinacoside (Nat'l Pharmaceutical & Biological Products Control Institute, lot number: 111670-200503), verbascoside (National Institute for Food and Drugs Control, lot number: 111530-200706), naringin (National Institute for Food and Drugs Control, lot number: 201111), neohesperidin (Nat'l Pharmaceutical & Biological Products Control Institute, lot number: 111857-201l02) inspection wavelength be respectively: 250nm, 330nm, 283nm, mobile phase: acetonitrile-0.1% formic acid solution (5:95-90:10 gradient elution), the inspection wavelength of Alisol B monoacetate (National Institute for Food and Drugs Control, lot number: 111846-201102): 208nm, mobile phase: acetonitrile-water (73:27), flow velocity: 1.0ml/min, column temperature: 30 DEG C, sample size: 10 μ l.
2. the preparation of " jichuan decoction " square Chinese medicine extraction matter sample
1) extract of the present invention
By obtained each 1 lot number of above-mentioned Chinese medicine extract respectively of the method described in example 1,2,3,4,5 of the present invention.
3. the preparation of standard solution
Get reference substance respectively appropriate containing ferulic acid Ferulic acid, Hesperetic acid 3-Hydroxy-4-methoxycinnamicacid, accurately weighed, put in brown bottle, add 70% methanol and make every 1ml containing ferulic acid 12 μ g, containing the solution of Hesperetic acid 20 μ g; Get reference substance β-ecdysterone (β-ecdyson) Hydroxyecdysone appropriate, accurately weighed, add methanol and make the solution of every 1ml containing 0.1mg; Get reference substance echinacoside Bchinacoside, verbascoside Verbascoside respectively appropriate, accurately weighed, add 50% methanol and make 1ml respectively containing the mixed solution of 0.2mg.Get reference substance Alisol B monoacetate Alisol B 23-acetate appropriate, accurately weighed, add acetonitrile and make the solution of every 1ml containing 20 μ g; Get reference substance naringin Naringin respectively, neohesperidin Neohesperidin reference substance is appropriate, accurately weighed, add methanol and make the solution of every 1ml containing naringin and each 80 μ g of neohesperidin respectively.
4. assay method
The above-mentioned Chinese medicine extraction matter sample getting each lot number is respectively appropriate, and accurately weighed about 25mg, is transferred in 50ml volumetric flask, adds methanol 40ml, ultrasonic 30min under room temperature, takes out cool to room temperature, adds methanol to scale, shake up, draw appropriate, be settled to 50ml, shake up, can measure.Each component content is calculated according to one point external standard method.The results are shown in Table 1:
The each constituent content measurement result of table 1 jichuan decoction extract
Therefore " jichuan decoction " square Chinese medicine extract, it contains by weight, and ferulic acid is not less than 0.02%, Hesperetic acid is not less than 0.036%, β-ecdysterone is not less than 0.011%, echinacoside is not less than 0.01%, Alisol B monoacetate 0.019%, naringin are not less than 1.4%.
Embodiment 8 " jichuan decoction " square Chinese medicine extract is to the effect of mice Intestinal pushing function
1) intestinal propellant movement function experiment
Choose healthy geriatric mice 70, body weight 20 ~ 22g, male and female half and half, be divided into 7 groups at random.Blank group: without medicine, only gives distilled water; Positive controls: phenolphthalein tablet; " jichuan decoction " square Chinese medicine extract 5 groups prepared by the embodiment of the present invention 1,2,3,4,5, totally 7 groups.By table 2 dosage to the continuous gavage of mice (ig) administration 12d, before determination experiment, water 16h is can't help in the fasting of each group mice.To each mice prepared Chinese ink gavage.From filling prepared Chinese ink, record every animal and arrange the melena time first, defecation grain number and water content in 6 hours.
2) small intestinal charcoal end Promoting Experiment
Choose healthy geriatric mice 70, body weight 20 ~ 22g, male and female half and half, be divided into 7 groups at random.Blank group: without medicine, only gives distilled water; Positive controls: phenolphthalein tablet; " jichuan decoction " square Chinese medicine extract 5 groups prepared by the embodiment of the present invention 1,2,3,4,5, totally 7 groups.By table 3 dosage to the continuous gavage of mice (ig) administration 12d, before determination experiment, water 16h is can't help in the fasting of each group mice.By charcoal end suspension (activated carbon powder containing 5%, 10% Radix Acaciae senegalis) gavage, after 25 minutes, de-cervical vertebra puts to death mice immediately, open abdominal cavity and be separated mesentery, large intestine upper end to pylorus is " total small intestinal length ", is " prepared Chinese ink propelling length " from pylorus to prepared Chinese ink forward position.Be calculated as follows ink progradation:
Weigh with scale the weight in wet base of small intestinal, then by small intestinal in baking oven, dry to constant weight for 80 DEG C, calculate the moisture of small intestinal.
3) statistical analysis
All experimental results all with show (± s) with t inspection organize between significant difference compare, be that difference has statistical significance with P<0.05.
4) result is affected on Aged Mice intestine movement function
The impact of " jichuan decoction " square Chinese medicine extract on Aged Mice intestine movement function the results are shown in Table 2.Arrange in the melena time first in Aged Mice, compared with blank group, all do not have significant difference, illustrate that each side is on treatment senile constipation, effect is gentle, can stimulate enterokinesia, not cause violent diarrhoea again, make defecation time be tending towards normal level.In Aged Mice 6 hours defecation numbers, the drug effect of " jichuan decoction " square Chinese medicine extract is obvious compared with blank group, namely there were significant differences (P<0.05), illustrates that jichuan decoction extract has the effect of the feces volume increasing mice in certain hour.
5) small intestinal charcoal end Promoting Experiment result
Small intestinal charcoal end Promoting Experiment the results are shown in Table 3.Compared with blank normal group, the charcoal end propelling rate of " jichuan decoction " square Chinese medicine extract group has remarkable increase (P<0.05), illustrate that this group has the effect of fairly obvious enhancing charcoal end propelling, the result of small intestinal water content experiment can be found out, in comparing with blank normal group and positive controls, " jichuan decoction " square Chinese medicine extract acts on all obviously (P<0.05) in this index of increase small intestinal water content.
Table 2 " jichuan decoction " square Chinese medicine extract is on the impact of Aged Mice intestine movement function
Note: compare with blank group, * P<0.05.Each concrete lot number is with table 1.
Table 3 " jichuan decoction " square Chinese medicine extract is on the impact of Aged Mice small intestinal charcoal end Promoting Experiment
Note: compare with blank group, * P<0.05.。Each concrete lot number is with table 1.
The square Chinese medicine extract antiobesity action of embodiment 9 " jichuan decoction "
Choose Kunming mouse 60, male and female half and half, body weight is between 18 ~ 22g.Mice is divided into three groups at random, and the body weight of three groups of mices, triglyceride and total cholesterol level zero difference, have comparability (P>0.05).
Blank group gavage every day normal saline 1mL, gives 1g/ml " jichuan decoction " square Chinese medicine extract lmL every day respectively.Successive administration 10 days, measures the body weight of mice, serum triglyceride level and serum total cholesterol for 10 days afterwards, calculates obesity index.
1) statistical analysis
All data all adopt SPSS13.0 to analyze, and continuous data adopts mean+SD to represent, adopt t inspection, P<0.05 represents that difference has statistical significance.
2) experimental result
A) body weight and obesity index
Through the gastric infusion of 10 days, body weight and the obesity index statistical result of matched group and " jichuan decoction " square Chinese medicine extract group mice are as shown in table 4, the body weight of control group mice can reach 44.34g, the body weight of " jichuan decoction " square Chinese medicine extract mice is respectively 40.67,37.89,38.53,38.12,36.34, compared with matched group, difference has statistical significance (P<0.05).The obesity index of " jichuan decoction " square Chinese medicine extract group mice is also starkly lower than matched group, obvious difference, has statistical significance (P<0.05).
Table 4 three groups of Mouse Weights compare with obesity index
Note: compare with matched group, * P<0.05.。Each concrete lot number is with table 1.
B) serum total cholesterol and triglyceride
Three groups of mice serum triglyceride and total cholesterol level more as shown in table 5, triglyceride and the total cholesterol level of " jichuan decoction " square Chinese medicine extract group are respectively 1.28,1.14,1.02,0.88,0.74 and 3.3,3.3,3.2,3.0,3.1, all be starkly lower than the index that matched group is corresponding, difference has statistical significance (P<0.05).
In this research, our high spot reviews " jichuan decoction " square Chinese medicine extract is to the fat-reducing effect of hyperlipidemic mice, research shows, " jichuan decoction " square Chinese medicine extract can reduce the body weight of Nutritive obesity mice significantly, obesity index, serum total cholesterol and triglyceride levels, compared with matched group, obvious difference, has statistical significance.As can be seen here, " jichuan decoction " square Chinese medicine extract has the pharmacologically active of blood fat reducing, can reach certain weight losing function, provides important evidence for taking " jichuan decoction " square Chinese medicine extract fat-reducing.
Table 5 three groups of mice triglyceride and total cholesterol level compare
Note: compare with matched group, * P<0.05.。Each concrete lot number is with table 1.
The acute toxicological experiment of embodiment 10 " jichuan decoction " square Chinese medicine extract
By acute toxicological experiment method, select kunming mice 200, fasting, can't help water 12h.Square to " jichuan decoction " Chinese medicine extract, (Example 1 lot number is 14010208 respectively; Embodiment 2 lot number is 14020524; Embodiment 3 lot number is lot number is 14030620; Embodiment 4 lot number is 14040927; Embodiment 5 lot number is lot number is 14051205).Be mixed with the suspension of Cmax separately, every each gavage of mice is about 0.9mL/20g (body weight), gavage 3 times in 24h, interval 4h.Then raise routinely, and observe the toxic reaction situation (outward appearance, activity, breathing, secretions, defecation, death etc.) of mice, and continuous weighting 7 days.The maximum administration concentration of result, maximum administration capacity, all do not measure median lethal dose(LD 50) (LD
50).
The acute toxicity tests shows, be somebody's turn to do " jichuan decoction " square Chinese medicine extract through gastric infusion approach, the appetite of ICR mice, body weight no abnormality seen, each lot number LD50 is all greater than 20.0g/kg, shows that toxicity is low, has good drug safety.
Claims (11)
1. the water extraction preparation method of jichuan decoction side's Chinese medicine extract, it is characterized in that, with Radix Angelicae Sinensis, Radix Achyranthis Bidentatae, Herba Cistanches, Rhizoma Alismatis, Rhizoma Cimicifugae, Fructus Aurantii for raw material, each raw material weight is than being Radix Angelicae Sinensis: Radix Achyranthis Bidentatae: Herba Cistanches: Rhizoma Alismatis: Rhizoma Cimicifugae: Fructus Aurantii=5:2:3:1.5:0.5:1; Preparation method comprises the steps: above-mentioned ingredients to clean, take raw material in proportion, add 13 ~ 16 times of water yields and soak 20-30min, heating extraction 3 times, first time extracts 1h, second time extracts 45min, third time extracts 30min, and each extraction time calculates from after boiling, merge extractive liquid, the concentrated extracting solution obtained is 6 ~ 8 times of described raw material weight to described extracting liquid volume, obtains initial concentration extracting solution; Being dried to relative density is further 1.0 ~ 1.2g/cm
3jichuan decoction side's Chinese medicine extract.
2. the alcohol extraction preparation method of jichuan decoction side's Chinese medicine extract, it is characterized in that, with Radix Angelicae Sinensis, Radix Achyranthis Bidentatae, Herba Cistanches, Rhizoma Alismatis, Rhizoma Cimicifugae, Fructus Aurantii for raw material, each raw material weight is than being Radix Angelicae Sinensis: Radix Achyranthis Bidentatae: Herba Cistanches: Rhizoma Alismatis: Rhizoma Cimicifugae: Fructus Aurantii=5:2:3:1.5:0.5:1; Preparation method comprises the steps: above-mentioned ingredients to clean, take raw material in proportion, add 13 ~ 16 times of 65% ~ 85% soak with ethanol 20-30min, heating and refluxing extraction 2 times, each 1h, merge extractive liquid, the concentrated extracting solution obtained to the volume of described extracting solution is 6 ~ 8 times of described raw material weight, obtains initial concentration extracting solution; Being dried to relative density is further 1.0 ~ 1.2g/cm
3jichuan decoction side's Chinese medicine extract.
3. the water extract-alcohol precipitation preparation method of jichuan decoction side's Chinese medicine extract, it is characterized in that, with Radix Angelicae Sinensis, Radix Achyranthis Bidentatae, Herba Cistanches, Rhizoma Alismatis, Rhizoma Cimicifugae, Fructus Aurantii for raw material, each raw material weight is than being Radix Angelicae Sinensis: Radix Achyranthis Bidentatae: Herba Cistanches: Rhizoma Alismatis: Rhizoma Cimicifugae: Fructus Aurantii=5:2:3:1.5:0.5:1; Preparation method comprises the steps:
1) concentrated solution is prepared: above-mentioned ingredients cleaned, take raw material in proportion, add 13 ~ 16 times of water yields and soak 20-30min, heating extraction 3 times, first time extracts 1h, second time extracts 45min, third time extracts 30min, and each extraction time calculates from after boiling, merge extractive liquid, the concentrated extracting solution obtained is 6 ~ 8 times of described raw material weight to described extracting liquid volume, obtains initial concentration extracting solution;
2) add alcohol settling: add ethanol in proper amount in the initial concentration extracting solution of above-mentioned preparation, make solvent alcohol content be 65 ~ 75%, leave standstill 24 hours, filter, filtrate recycling ethanol, obtains refined liquid, concentrated, is drying to obtain jichuan decoction side's Chinese medicine extract.
4. the purification by macroporous resin preparation method of jichuan decoction side's Chinese medicine extract, it is characterized in that, with Radix Angelicae Sinensis, Radix Achyranthis Bidentatae, Herba Cistanches, Rhizoma Alismatis, Rhizoma Cimicifugae, Fructus Aurantii for raw material, each raw material weight is than being Radix Angelicae Sinensis: Radix Achyranthis Bidentatae: Herba Cistanches: Rhizoma Alismatis: Rhizoma Cimicifugae: Fructus Aurantii=5:2:3:1.5:0.5:1; Preparation method comprises the steps:
1) concentrated solution is prepared: above-mentioned ingredients cleaned, take raw material in proportion, add 13 ~ 16 times of water yields and soak 20-30min, heating extraction 3 times, first time extracts 1h, second time extracts 45min, third time extracts 30min, and each extraction time calculates from after boiling, merge extractive liquid, the concentrated extracting solution obtained is 6 ~ 8 times of described raw material weight to described extracting liquid volume, obtains initial concentration extracting solution;
2) purification by macroporous resin: add respectively in the initial concentration extracting solution that 3 ~ 5 times of volume hot water obtain to upper step, get supernatant; Supernatant is crossed macroporous resin column absorption, abandoned stream fluid; Then use the water of 1 ~ 5BV to wash described adsorption column with the flow velocity of 0.5 ~ 2.5BV/ hour, abandon water lotion; Adopt the ethanol of 50 ~ 70% afterwards, elution volume 3 ~ 5 BV, with the flow velocity eluting adsorption column of 0.5 ~ 2.5BV/ hour, collect this ethanol elution, concentrated, dry described ethanol elution, obtain jichuan decoction side's Chinese medicine extract.
5. the purification by macroporous resin preparation method of jichuan decoction side's Chinese medicine extract, it is characterized in that, with Radix Angelicae Sinensis, Radix Achyranthis Bidentatae, Herba Cistanches, Rhizoma Alismatis, Rhizoma Cimicifugae, Fructus Aurantii for raw material, each raw material weight is than being Radix Angelicae Sinensis: Radix Achyranthis Bidentatae: Herba Cistanches: Rhizoma Alismatis: Rhizoma Cimicifugae: Fructus Aurantii=5:2:3:1.5:0.5:1; Preparation method comprises the steps:
1) concentrated solution is prepared: according to above-mentioned prescription proportioning, with 65% ~ 85% ethanol for raw material described in solvent extraction, above-mentioned ingredients is cleaned, take raw material in proportion, add 13 ~ 16 times of 65% ~ 85% soak with ethanol 20-30min, heating and refluxing extraction 2 times, each 1h, merge extractive liquid, the concentrated extracting solution obtained to the volume of described extracting solution is 6 ~ 8 times of described raw material weight, obtains initial concentration extracting solution;
2) purification by macroporous resin: add respectively in the initial concentration extracting solution that 3 ~ 5 times of volume hot water obtain to upper step, get supernatant; Supernatant is crossed macroporous resin column absorption, abandoned stream fluid; Then use the water of 1 ~ 5BV to wash described adsorption column with the flow velocity of 0.5 ~ 2.5BV/ hour, abandon water lotion; Adopt the ethanol of 50 ~ 70% afterwards, elution volume 3 ~ 5 BV, with the flow velocity eluting adsorption column of 0.5 ~ 2.5BV/ hour, collect this ethanol elution, concentrated, dry described ethanol elution, obtain jichuan decoction side's Chinese medicine extract.
6. the preparation technology of jichuan decoction side's teabag, is characterized in that, with Radix Angelicae Sinensis, Radix Achyranthis Bidentatae, Herba Cistanches, Rhizoma Alismatis, Rhizoma Cimicifugae, Fructus Aurantii for raw material, each raw material weight is than being Radix Angelicae Sinensis: Radix Achyranthis Bidentatae: Herba Cistanches: Rhizoma Alismatis: Rhizoma Cimicifugae: Fructus Aurantii=5:2:3:1.5:0.5:1; Preparation method comprises the steps: above-mentioned ingredients to clean, and takes raw material in proportion, is ground into coarse granule, coarse granule is distributed into pouch-packaged, make teabag.
7. the preparation technology of jichuan decoction side's teabag, is characterized in that, with Radix Angelicae Sinensis, Radix Achyranthis Bidentatae, Herba Cistanches, Rhizoma Alismatis, Rhizoma Cimicifugae, Fructus Aurantii for raw material, each raw material weight is than being Radix Angelicae Sinensis: Radix Achyranthis Bidentatae: Herba Cistanches: Rhizoma Alismatis: Rhizoma Cimicifugae: Fructus Aurantii=5:2:3:1.5:0.5:1; Preparation method comprises the steps: above-mentioned ingredients to clean, and takes raw material in proportion, is ground into coarse granule, coarse granule and Flos Lonicerae is dressed up pouch-packaged by the weight fraction of 13:1 ~ 13:5, makes teabag.
8. according to the preparation method in claim 1-5 described in any one method, it is characterized in that, described water or soak with ethanol time are 25min.
9. the jichuan decoction side's Chinese medicine extract obtained as any one method preparation in claim 1-5, it is characterized in that, containing ferulic acid, Hesperetic acid, β-ecdysterone, echinacoside, Alisol B monoacetate, naringin in described extract, by weight, ferulic acid is not less than 0.02%, Hesperetic acid is not less than 0.036%, β-ecdysterone is not less than 0.011%, echinacoside is not less than 0.01%, Alisol B monoacetate is not less than 0.019%, naringin is not less than 1.4%.
10. the pharmaceutical composition of the jichuan decoction side's Chinese medicine extract obtained containing, for example any one method preparation in claim 1-5, it is characterized in that, described compositions is oral formulations, and described oral formulations comprises pharmaceutically acceptable carrier and/or excipient.
11. as any one method preparation in claim 1-5 jichuan decoction side's Chinese medicine extract of obtain preparation treat Senile Functional Constipation, puerperal blood deficiency and constipation and slimming medicine or health product in application.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510066582.8A CN104706855A (en) | 2015-02-09 | 2015-02-09 | Preparation methods and application of Jichuan decoction traditional Chinese medicine extract |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510066582.8A CN104706855A (en) | 2015-02-09 | 2015-02-09 | Preparation methods and application of Jichuan decoction traditional Chinese medicine extract |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104706855A true CN104706855A (en) | 2015-06-17 |
Family
ID=53406899
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510066582.8A Pending CN104706855A (en) | 2015-02-09 | 2015-02-09 | Preparation methods and application of Jichuan decoction traditional Chinese medicine extract |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104706855A (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105028805A (en) * | 2015-06-17 | 2015-11-11 | 陈云勇 | Preparation method for artemisia anomalas tea bag |
| CN105193967A (en) * | 2015-09-12 | 2015-12-30 | 南京正亮医药科技有限公司 | Method for extracting plant composition containing cimicifugae foetidae and application |
| CN106975021A (en) * | 2016-12-14 | 2017-07-25 | 江苏海王健康生物科技有限公司 | A kind of preparation method and applications of enriching yin Lonicera and Forsythia soup side antivirus extract |
| CN108459130A (en) * | 2017-02-17 | 2018-08-28 | 华润三九医药股份有限公司 | The method for building up and finger-print of jichuan decoction finger-print |
| CN108578541A (en) * | 2018-07-06 | 2018-09-28 | 海王（湖北）中医药研究总院有限公司 | A kind of Chinese medical extract that treating rheumatism and cardiovascular and cerebrovascular disease, preparation method and applications |
| CN108743893A (en) * | 2018-06-15 | 2018-11-06 | 海王（湖北）中医药研究总院有限公司 | The preparation method and applications of clearing lung-heat soup side's Chinese medical extract |
| CN108888686A (en) * | 2018-07-06 | 2018-11-27 | 海王（湖北）中医药研究总院有限公司 | It is a kind of to treat stomachache, hypochondriac pain, gastroenteritic ulcer, the Chinese medical extract of depression and its preparation method and application |
| CN109172731A (en) * | 2018-07-27 | 2019-01-11 | 三亚海王海洋生物科技有限公司 | A kind of preparation method and applications of oyster compound extract that treating cough, essential hypertension and apoplexy |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH06116258A (en) * | 1992-10-06 | 1994-04-26 | Nikken Food Kk | Production of low-caffeine green tea leaf catechins with low-caffeine content |
| CN101284051A (en) * | 2008-02-25 | 2008-10-15 | 北京金方华医药科技有限公司 | Ji Chuan Jian oral preparation and preparation method thereof |
| CN102258646A (en) * | 2011-07-06 | 2011-11-30 | 陕西中药研究所 | Preparation method of Jichuan pills |
| CN102430014A (en) * | 2011-12-07 | 2012-05-02 | 哈药集团中药二厂 | Preparation method of oral preparation of jichuan-decoction granules |
-
2015
- 2015-02-09 CN CN201510066582.8A patent/CN104706855A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH06116258A (en) * | 1992-10-06 | 1994-04-26 | Nikken Food Kk | Production of low-caffeine green tea leaf catechins with low-caffeine content |
| CN101284051A (en) * | 2008-02-25 | 2008-10-15 | 北京金方华医药科技有限公司 | Ji Chuan Jian oral preparation and preparation method thereof |
| CN102258646A (en) * | 2011-07-06 | 2011-11-30 | 陕西中药研究所 | Preparation method of Jichuan pills |
| CN102430014A (en) * | 2011-12-07 | 2012-05-02 | 哈药集团中药二厂 | Preparation method of oral preparation of jichuan-decoction granules |
Non-Patent Citations (2)
| Title |
|---|
| 何国梁: "《方剂学应试指南》", 30 June 1999, 广东科技出版社 * |
| 路军章等: "《中华传世名方实用手册》", 31 January 2011, 年龄出版社 * |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105028805A (en) * | 2015-06-17 | 2015-11-11 | 陈云勇 | Preparation method for artemisia anomalas tea bag |
| CN105028805B (en) * | 2015-06-17 | 2018-10-09 | 陈云勇 | The preparation method of artemisia anomalas tea bag |
| CN105193967A (en) * | 2015-09-12 | 2015-12-30 | 南京正亮医药科技有限公司 | Method for extracting plant composition containing cimicifugae foetidae and application |
| CN106975021A (en) * | 2016-12-14 | 2017-07-25 | 江苏海王健康生物科技有限公司 | A kind of preparation method and applications of enriching yin Lonicera and Forsythia soup side antivirus extract |
| CN108459130A (en) * | 2017-02-17 | 2018-08-28 | 华润三九医药股份有限公司 | The method for building up and finger-print of jichuan decoction finger-print |
| CN108459130B (en) * | 2017-02-17 | 2020-05-05 | 华润三九医药股份有限公司 | Jichuan decoction fingerprint establishing method and fingerprint |
| CN108743893A (en) * | 2018-06-15 | 2018-11-06 | 海王（湖北）中医药研究总院有限公司 | The preparation method and applications of clearing lung-heat soup side's Chinese medical extract |
| CN108578541A (en) * | 2018-07-06 | 2018-09-28 | 海王（湖北）中医药研究总院有限公司 | A kind of Chinese medical extract that treating rheumatism and cardiovascular and cerebrovascular disease, preparation method and applications |
| CN108888686A (en) * | 2018-07-06 | 2018-11-27 | 海王（湖北）中医药研究总院有限公司 | It is a kind of to treat stomachache, hypochondriac pain, gastroenteritic ulcer, the Chinese medical extract of depression and its preparation method and application |
| CN109172731A (en) * | 2018-07-27 | 2019-01-11 | 三亚海王海洋生物科技有限公司 | A kind of preparation method and applications of oyster compound extract that treating cough, essential hypertension and apoplexy |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104706855A (en) | Preparation methods and application of Jichuan decoction traditional Chinese medicine extract | |
| CN101612362B (en) | Complex preparation for regulating lipid and preventing and curing heart cerebrovascular diseases and preparation method thereof | |
| CN106975021A (en) | A kind of preparation method and applications of enriching yin Lonicera and Forsythia soup side antivirus extract | |
| CN108743892A (en) | The preparation method and applications of " Shengyang Yiwei Decoction " side's Chinese medical extract | |
| CN102145106B (en) | Chinese medical composition for treating osteoporosis and granular formulation capable of tonifying kidney and invigorating blood circulation | |
| CN102475830B (en) | Medicinal composition for treating coronary disease and angina pectoris, preparation method thereof and preparation thereof | |
| CN102078543B (en) | Traditional Chinese medicine preparation for treating gout and preparation method thereof | |
| CN102120020B (en) | Medicinal composition for treating hyperplastic diseases of breast and hysteromyoma and preparation method thereof | |
| CN101474275B (en) | Preparation method and application of Chinese medicine extract for 'prescription for treating diarrhoea with abdominal pain' | |
| CN101156918B (en) | A medicine for treating gout and preparation method | |
| CN102526230B (en) | Traditional Chinese composition for curing liver disease and preparation method, quality detection method and application thereof | |
| CN102961584B (en) | Traditional Chinese medicine composition capable of relieving fatigue, as well as preparation method and application thereof | |
| CN104069194B (en) | A kind of Chinese medicine composition with antitumaous effect and its production and use | |
| CN102670711B (en) | White flower salviae miltiorrhizae extract for curing angiitis, preparation method and application | |
| CN104352940B (en) | A kind of Chinese medicine composition for alleviating physical fatigue | |
| CN103919845B (en) | A kind of pharmaceutical composition for improving anoxia endurance and its preparation method and application | |
| CN102805836A (en) | Chinese traditional medical composition for treating primary hepatic carcinoma and preparation method thereof | |
| CN102125589B (en) | Preparation method and use of active ingredients of red ginseng | |
| CN108578541A (en) | A kind of Chinese medical extract that treating rheumatism and cardiovascular and cerebrovascular disease, preparation method and applications | |
| CN103877513A (en) | Chinese herbal compound composition with blood fat reduction effect, and applications thereof | |
| CN116999533B (en) | Heart-nourishing and pulse-activating granule, preparation method thereof and application thereof in antidepressant product | |
| CN100502912C (en) | Medicine for treating deficiency syndrome, its preparing process and usage | |
| CN102008587A (en) | Chinese medicinal preparation for treating liver cancer and preparation method thereof | |
| CN101700323B (en) | Pharmaceutical composition for preventing and treating liver cancer | |
| CN1994426B (en) | Preparation method, and identification and assaying method of 'Sheng Mai' soft capsule |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20150617 |