CN1947488A - Method for culture of alpinia galanga and its quality-control - Google Patents
Method for culture of alpinia galanga and its quality-control Download PDFInfo
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Abstract
A method for culturing the rhizome of alpinia galanga includes such steps as tissue culture, transplanting, field management, applying fertilizer, controlling diseases and pests and harvesting. Its processing, transportation and storage are also disclosed. Its quality control method includes UV spectrophotometry, thin-layer chromatography, gas-phase chromatographic fingerprint, and efficient liquid-phase chromatography for measuring the content of 1'-acetoxychavicol acetate.
Description
Technical field
The kind bacterium that the present invention relates to the rhizome galanga galangal rhizome of Zingiber Zingiberaceae Alpinia plants Galanga Galangal Seed Alpinia galanga willd. breeds, the processing of gathering, packing, transportation, storage and the method for quality control of transplant planting, field management, fertilising, extermination of disease and insect pest cultivation method and galanga galangal rhizome.
Background technology
Galanga galangal rhizome has another name called Alpinia galanga, galingal, Alpinia japonica, Alpinia japonica head (Zhanjiang, Guangdong), honest and clean ginger (Guangdong, Guangxi), sees Fig. 1, is the rhizome of the Galanga Galangal Seed Alpinia galangawilld. of Zingiber Zingiberaceae Alpinia plants, and its biological property is as follows:
One, phytomorph (seeing Fig. 2,3,4,5,6,7)
1, habit: the perennial draft of growing thickly, high 1.5-2.5m.The florescence 6-7 month, the fruit phase 7-10 month.
2, rhizome: sturdy, circle has joint, and light brown red and hot taste slightly have fragrance slightly.
3, leaf: 2 row; No petiole or extremely short; The long 5-10mm of the tip of a leaf, tip is blunt; The blade Long Circle is wide lanceolar extremely, long 25-50cm, and wide 5-10cm, the anxious point of tip has mucro, and base portion is gradually narrow, and the normal palm fibre in edge is white, does not have the hair or the back side long pubescence is arranged, and the edge is filbert when doing.
4, inflorescence: give birth on the panicle top, and upright, close life is spent more, long 14-32cm, footpath 4-8cm; Close living pubescence on the rhachis, branch is many and lack, and spends 3～6 on each branch; Phyllary is linear, is about 20cm; Bractlet lanceolar or long and narrow circle, long 1-2cm.
5, flower: green white, delicate fragrance; 3 shallow the splitting that calyx tubulose, long 7-10mm, tip do not wait have echinid; Corolla Guan Yue is slightly isometric, sliver 3, and Long Circle, long 12-16mm, wide 3-5mm, the top sliver is wide slightly, tip root and stem of certain plants shape; The lip obovate is to Long Circle, long 2.5-3cm, and wide 8-12mm, tip dark 2 splits to the middle part, and base portion is shrunk to claw-like, and central authorities slightly thicken and red streak are arranged; Stamen 1, isometric with lip, flower pesticide Long Circle, tip do not have the connective adjunct; 2 pieces of staminodiums, lanceolar, long 6-10mm the base portion of being born in lip; The gynoecium ovary is the next, does not have hair; Style is elongated, and column cap protrudes in the tip of flower pesticide.
6, fruit: the capsule Long Circle, do not ftracture, long 11-16mm, footpath 7-9mm, slightly shrink at the middle part, and there is the place calyx on the top, and is orange red when ripe.
7, seed: polygonal, brownish black.
Two, habit of growth
Galanga galangal rhizome (Galanga Galangal Seed) adaptability is strong, happiness warm and humid climate environment, the low temperature that ability is of short duration about 0 ℃, drought-enduring slightly, be afraid of waterlogging, be born in shady wet sylvan life of hill cheuch or the shrub and in the thick grass, grow in the torrid zone, subtropical climate area, mainly be distributed in province, districts such as Guangdong, Guangxi, Hainan, Taiwan, Yunnan in China, see Fig. 8,9.
General average temperature of the whole year is equal energy normal growth more than 15 ℃, but the most suitable with 22 ℃-28 ℃ of average temperatures of the whole year; Optimum growth when general annual rainfall 1500-2000 millimeter, relative air humidity 80%-90%, soil moisture content 25%-30%.Soil with the plantation of loose, fertile, deep, well-drained loam or clay for well.
When summer, be afraid of that the sun directly shines, and is afraid of frost winter seedling stage.
Three, the feature of growing
Can constantly take out the 1-5 month and give birth to new bamboo shoot, in the majority with the 4-5 month, the pregnant flower bud that ears the 6-7 month, the 7-8 month blooms, 8-9 month result, 11-12 month fruit maturation.
Galanga galangal rhizome (Galanga Galangal Seed) is recorded in " property of medicine opinion " the earliest.According to book on Chinese herbal medicine record afterwards, have the situation that galanga galangal rhizome (Galanga Galangal Seed) and this platymiscium (particularly with galangal) are used with ancient times, book on Chinese herbal medicine thinks that Galanga Galangal Seed and galangal derive from same plant more, Galanga Galangal Seed is a fruit, galangal is a root, as: " Kaibao Bencao " says " Galanga Galangal Seed is given birth to South Sea Zhu Gu, and galangal is also ... ", " Classified Materia Meidca " says " Galanga Galangal Seed ... cloud is a FRUCTUS GALANGAE ... " etc., so that Compendium of Material Medica is merged into one to Galanga Galangal Seed and galangal.
According to textual criticism, use galanga galangal rhizome (Galanga Galangal Seed) and this platymiscium (particularly with galangal) ancient times with, and the galangal medicinal material in ancient times (Rhizoma Alpiniae Officinarum) mainly is the rhizome (Rhizoma Alpiniae Galanga) of the galanga galangal rhizome of today.Modern relevant investigation also shows: at present galanga galangal rhizome and beak Zingiber zerumbet Rhynchanthus beesinanus, intelligence development Alpinia oxyphyllaMiq., often together mixed up the galangal application apart from the rhizome of Zingiber zerumbet A.calcarataRosc..
According to the documents and materials record, institute of Materia Medica,Chinese Academy of Medical Sciences Chen Min henry etc. discovers that homemade Galanga Galangal Seed Alpinia galanga willd. compares with external kindred plant, the lip drastic crack that China produces, and middle part of fruit is shunk more obvious; Split and how shallow external lip is, middle part of fruit is not shunk or is not obvious.
The sample of gathering in Guangxi according to Chen Minheng etc., find that Galanga Galangal Seed Alpinia galanga willd. has two types, a kind of is the type that blade back and rhachis have pubescence, is decided to be mutation hair Galanga Galangal Seed Alpiniagalanga willd.Var.pytamidata (Blume) k.Schum.; Another kind is that the tip of medicine is about 4-7mm, is lance-shaped connective appendage, is decided to be the attached sharp Galanga Galangal Seed Alpinia galangawilld.Var.appendiculata Y.H.Chen of mutation, nov.var.ined.In quantity, these two mutation are all few, and it is also wideless to distribute.Rhizome as for these two mutation does not still have research and report at aspects such as pharmacognosy, quality, output.
In China, to do " Galanga Galangal Seed " except being used for picking fruit on a small quantity, galanga galangal rhizome is mainly by the making that is used for diet condiment among the people.Research report to galanga galangal rhizome shows in recent years, that the galanga galangal rhizome extract has is antiviral, antitumor, resistive connection nuclear, antiulcer, pharmacological action such as antibiotic, may become new resources of medicinal plant, but do not see artificial planting at present, the main wild resource that relies on, gather processing method and the method for quality control that also do not have standard, thereby Chang Yuyi adulterant such as intelligence development, galangal, use with apart from flower Alpinia japonica etc., galanga galangal rhizome is restricted as the application of medicinal plant.
Goal of the invention
The objective of the invention is to disclose a kind of galanga galangal rhizome standardized cultivation and gather processing, packing, transportation, storage practice and method of quality control, make the galanga galangal rhizome wild plant resource become tame product, for the pharmacognostical study of galanga galangal rhizome and develop new resources of medicinal plant foundation is provided.
Summary of the invention
For achieving the above object, the present invention is method cultivation galanga galangal rhizomes such as kind of a bacterium breeds, transplant planting, field management, fertilising, the extermination of disease and insect pest by adopting, and select collecting time, method and galanga galangal rhizome method of quality control such as collecting method and place of production processing, packing, storage according to the purposes of galanga galangal rhizome.
The concrete mode of implementing
One, seedling breeding
1, selects with growing seedlings
Usually be chosen in hillside, the small stream limit of certain concealment condition, irrigation and drainage are convenient, the section of easy grade of leeward, fertile soil on the sunny side with growing seedlings.Surgery weedtree, weeds and incinerate on the spot and make base manure, open up wasteland the every mu of farmyard manure 2000-2500 kilogram that applies through becoming thoroughly decomposed in back, play the smooth both implantable rhizome of using as seedling of furrow or broadcast into seed.
2, seedling-cultivating method
(1) rhizome breeding
1. select rhizome to select 2-3 to give birth to sturdy, 5-6 bud of band, no damage by disease and insect, more big and fleshy tender root-like stock, be cut into and be about 15 centimetres, have the segment that 2-3 saves.
2. mating season is usually at spring and autumn, carries out when gathering galanga galangal rhizome.
3. propagation method is opened the cave plantation by 30 * 25 centimetres of seeding row spacings on whole good soil, every cave kind 1-2 section, and normal root water is watered in compacting slightly behind the earthing.Every mu about 100 kilograms with rhizome.
(2) seminal propagation
1. the maternal plant of selecting to reserve seed for planting selects the plant of the no damage by disease and insect that the fruit grain is big, full, flavor is dense, output is high to be the maternal plant of reserving seed for planting.
2. the seed of seed collecting and seed treatment galanga galangal rhizome is generally ripe successively in the 10-12 month, when pericarp becomes redness, gathers in batches.Choose grain big, full, be orange red and do not have the fresh fruit of damage by disease and insect, peel off pericarp, the fine sand of sneaking into equivalent is rubbed to seed and fruit flesh and separate, the kind micromicro has slight damage, the permeability with the increase seed is beneficial to seed sprouting and emerges.With clear rinsing fine sand and fruit flesh, pull seed out, it is standby to dry moisture (seed is difficult for tanning by the sun).
3. sowing time in sowing time, the 4-5 month was best, can sow in batches.
4. type of seeding is laterally ditched with 20 centimetres line-spacings on whole good seedbed, and dark about 2 centimetres, wide 6 centimetres, the seed of handling well evenly is sprinkling upon in the ditch, earthing is a little more than the furrow face, and fine day waters and preserves moisture, the lid grass.Generally growing seedlings need be after half a year, the ability portable.
(3) tissue culture
With fresh galanga galangal rhizome is explant, be seeded in after the sterilization in MS+6-BA 2.0～10.0mg/L+NAA0.05～0.5mg/L medium and carry out inducing of young shoot, in time change induced bundle bud in MS+6-BA 1.0～3.0mg/L+NAA0.05～0.3mg/L medium after sprouting over to, change at last in MS+NAA0.01～0.5mg/L cultivation and carry out seedling breeding after the root induction.
(4) management with growing seedlings
1. the tender seedling incompatibility of galanga galangal rhizome of shading intense light irradiation requires to have certain concealment condition.Therefore, grow seedlings the initial stage, can cover grass and shade.Behind the planting seed, should keep furrow face ground moistening.After the germination, should in time throw off lid grass, and suitably set up cool canopy and shade, and note frequent trickle.
2. trickle after planting according to weather condition, can suitably water, to keep moistening.
3. weeding in time weeds out the rank grass, and accomplishes that the seedbed do not have weeds.
4. thinning is stayed a little less than going by force when seedling grows 3-6 centimetre, and making the strain spacing is 4 centimetres.
5. fertilising came up back about one month, converted 100 kilograms of mixing of water with 0.5 kilogram of urea and applied, and imposed ash; Go into then can execute before the winter pig cow dung become thoroughly decomposed to improve the tolerance to cold of seedling.
Two, transplant planting
1, choosing ground and whole ground
Because of the galanga galangal rhizome happiness torrid zone, subtropical climate, anti-morning not anti-frost.Require deep, loose, fertile acidity or the subacidity sand loam of soil layer.So that suitable gather is irritated is convenient, soil layer is deep, fertile, plant to loose hillside fields or gentle slope.Also can be under shelter forest, or the fruit tree underwood planting.Next year remove foreign material winter,, pick up clean stone, tree root, grass roots, plough deeply more than 30 centimetres, allow soil-ripening the Ex-all of weeds shrub, and sufficient base manure down, every mu of farmyard manure that becomes thoroughly decomposed that applies the 2000-2500 kilogram is made base manure, does not need bedding.Hack leveling again before the plantation in next year.
2, the field planting time
Should be fine morning or overcast and rainy the carrying out of the 3-4 month.
3, planting density
The seeding row spacing of galanga galangal rhizome plantation is advisable with 100 * 100 centimetres.
4, field planting method
Open the cave by seeding row spacing on whole good furrow, the specification in cave is 40 * 40 * 30 centimetres.When the seed height of seedling is more than 20 centimetres, go out the garden field planting, every cave kind 2-3 strain seedling, or 1 root-like stock of every cave kind, the bud head upwards bankets while putting, and plants back earthing compacting, and then covers fine earth 5-6 cm thick.
Three, field management
1, intertillage, weeding and ridging
Weeding in every month 1 time when not having the envelope row in early stage, each weeding of Feng Hanghou autumn in summer 1 time.Loosen the soil in passing during weeding, be beneficial to the sprouting and the growth of new root.Loosening the soil with plough or hoe trench digging around plant in the plantation back on the 2nd year, earths up simultaneously; Or stop up at the plant base portion with farmyard manure and table earth culture in conjunction with clear garden in the time of the year when autumn changes into winter, to promoting growth, quicken to sprout favourable.
2, irritate draining
Arid the time is watered or is irrigated, and to keep ground moistening, promotes plant tillering and root growth.
Four, fertilising
Fertilising is one of galanga galangal rhizome standardized planting process the key link, should be principle not pollute plant, soil and galanga galangal rhizome content of beary metal are exceeded standard at the soil characteristic in each base, the fertility assessment situation of soil and growth characteristic, psychological need, the medicinal part of galanga galangal rhizome itself.
1, the assessment of soil fertility
Can adopt " multidraw method at random ", press " conventional method of analysis of soil agricultural " that Soil Science Society of China agrochemistry Professional Committee works out, the soil in standardized planting base be carried out the mensuration of basic fertility; And further adopt improved Nei Meiluo (Nemoro) composite index law, and calculate the comprehensive fertility FACTOR P, carry out the overall merit of soil fertility.(seeing Table 1,2)
Table 1. soil fertility detects index and method
| Sequence number | Index | | Content | |
| 1 | Organic | Electric hot plate heating-K2Cr20 volumetric method | ||
| 2 | Full nitrogen | The Kelvin method | ||
| 3 | Hydrolyzable nitrogen | The alkaline hydrolysis diffusion process | ||
| 4 | Full phosphorus | Sour molten-molybdenum antimony resistance | ||
| 5 | Organic phosphor | 0.5MNaHCO3 lixiviate-molybdenum antimony resistance colorimetric method | ||
| 6 | Full potassium | NaOH fusion-flare photometer | ||
| 7 | Available potassium | 1NNH40Ac lixiviate-flare photometer | ||
| 8 | The trace element effective dose (Cu, Zn, Fe, Mn) | 0.1HCLmol/l lixiviate-atomic absorption spectrophotometry | ||
| 9 | Replacement property calcium magnesium (Ca, Mg) | 1mol/l ammonium acetate lixiviate-atomic absorption spectrophotometry |
Table 2. soil fertility FACTOR P and soil fertility relation table
| Sequence number | The comprehensive fertility FACTOR P | Fertility evaluation | The |
| 1 | ≥2.7 | Soil is very fertile | |
| 2 | 2.7-1.8 | Fertile soil | |
| 3 | 1.8-0.9 | Soil fertility is general | |
| 4 | ＜0.9 | Soil depletion |
2, work out the fertilising scheme
According to the nutrient demand characteristics of the fertility state and the galanga galangal rhizome different growth stage of soil,, formulate seedling stage, concrete fertilising scheme of whole growth cycle with reference to " the traditional Chinese medicine standardization is produced and allowed fertilizer type and the using priciple used ".
When selecting fertilizer, can be by carrying out the fertilizer test of different fertilizer kind, research is observed the different fertilizer kind to the influence of galanga galangal rhizome growth, to the plant nutrition effect of accumulation, to the influence of rhizome mineral nutrient accumulation, to the influence of plant strain growth and output etc.
Five, the extermination of disease and insect pest
The survey showed that, and the damage by disease and insect of galanga galangal rhizome is less, and main disease is the root stem rot, and main insect pest is flat snail.
1, the root stem rot claims root rot again, sees Figure 10.
(1) symptom
The main harm root.The root blackening of catching an illness of supporting root and fibrous root is rotted.And expand to main root.Main root is caught an illness and just produce irregular blackspot on the root skin, and constantly expansion, causes most of root blackening, to the xylem expansion, causes whole root-rot mashed, and the diseased plant growth is weak, the little jaundice of leaf, and plant is wilted until withered.This disease mostly occurred in high temperature season or rainy season, also morbidity easily under the many conditions of ponding.Take place rather seriously during continuous cropping, pathogen can survive the winter at soil and old complaint, and soil carries disease germs can its spread in china.Also can propagate for media via farm implements of planting pollution or irrigation water etc.
(2) cause of disease Fusarium solani (Mart.) Sacc. claims eggplant class sickle spore, belongs to the Deuteromycotina fungi.
(3) occurrence regularity
On invalid or in the soil or enter in the fertilizer and survive the winter, invade through wounds such as insect bite, mechanical injury, nematode wounds by germ with sclerotium, chlamydospore for germ.
(4) prevent and treat method
Integrated pest control in agriculture is enhanced field management, and improves surrounding environment, carries out work such as ventilation, printing opacity, draining, improves the resistance against diseases of plant self, reduces the generation of damage by disease and insect.Their early stage is pulled out diseased plant, and with the pulverized limestone sterilization, avoids pathogen transmission as far as possible.Simultaneously, irritate the root control with 0.2～0.4 Baume Lime Sulphur (bordeaux mixture).
2, flat snail
(1) formal name used at school Bradybaena similaris (Ferussac)
(2) damage symptom is an omnivorousness software animal, and happiness food plant tender tissue as positions such as tender leaf, sprouting, butts, causes irregular scar or hole.
Come into play when (3) the pests occurrence rule spring is warm, like the dark and damp environment that shelters from heat or light that has, cool or haunt overcast and rainy moist daytime night, especially the activity of summer high temperature pluvial period is the most frequent, and it is rapider to grow.
(4) method of preventing and treating is enhanced field management, the cleaning planting environment, and health arround the attention weeds a garden.Can select suitable agricultural chemicals to prevent and treat, for example 6% carbaryl-methaldehyde with reference to " pesticide variety and the using priciple that allows and ban use of produced in the traditional Chinese medicine standardization " (seeing Appendix 4).
Six, the processing of gathering
1, gathers
(1) collecting time is according to the accumulation dynamic of galanga galangal rhizome yield per unit area, active ingredient, and the factors such as experience, conversion in season of gathering with reference to tradition are determined out its picking time, determine that the time of gathering is 2～March.
(2) collecting method is selected fine day, cuts off acrial part stem, leaf earlier, root-like stock has been dug one by one collected.
2, place of production processing
With the rhizome of results, remove acrial part, earth, fibrous root and scale, using fresh herb or be cut into 5～6 centimetres segment, clean, segment, dry.
Seven, galanga galangal rhizome quality control
(1) proterties
The dry rhizome of galanga galangal rhizome is cylindric, multi-branched, long 8-12cm, diameter 1.2-3.0cm.Brown or the mulberry of surface red, the vertical wrinkle of tool has the capable faint yellow leaf scar of wave, forms link, the long 1-3cm of internode, the rhizome downside has mark of fibrous root.Matter is tough and tensile, frangibility not, and section fibrous, faint yellow, skin zone accounts for 2/3, and endodermis is obvious, and the vascular bundle asterism is as seen.Normal and the skin portion of woody part from, look darker.Gas fragrance, it is little pungent to distinguish the flavor of.
(2) differentiate
1, micro-discriminating
(1) rhizome cross section
1. epidermal cell 1 row, class is square, and outward by cuticula, what have contains brown thing, sees Figure 11.
2. the cortex broadness is the multiple row parenchyma cell, and loosing has most vascular bundles and leaf (root) mark vascular bundle, sees Figure 11,12.
3. the outer tough type of vascular bundle, 2～7 in conduit, bundle sheath fiber 2～4 row; Leaf (root) mark vascular bundle is radial scale leaf or the fibrous root of passing to, and sees Figure 14,12.
4. endodermis cell 1 is listed as, and square, marshalling is seen Figure 15.The outer tough type of center pillar vascular bundle, more, the intensive Cheng Huan in inner cortex place, the middle part hash is seen Figure 16.Loosing in the parenchyma has class polygonal secretory cell, includes brown thing, sees Figure 13.Parenchyma cell contains starch grain.
(2) powder
1. powder is pale red brown, gas perfume (or spice), and it is little pungent to distinguish the flavor of.
2. starch grain is numerous, simple grain, Long Circle, long avette; Greatly different in size, omphalion and laminated striation are not obvious, see Figure 17.
3. fibre debris is seen more, wall thickness, on oblique pit is arranged, side Guan Bi is beaded, sees Figure 18,19.
4. the parenchyma cell similar round is seen Figure 20 to Long Circle.
5. secretory cell class polygonal includes brown thing, sees Figure 21.
6. epidermal cell class polygonal, wall is beaded slightly, visible pit, what have contains brown thing, sees Figure 22.
7. the many scalariforms of conduit, reticulate pattern are seen Figure 23,24.
2, physics and chemistry is differentiated
(1) need testing solution preparation
Get galanga galangal rhizome fine powder 1g, put in the 50ml band plug erlenmeyer flask, the 20ml that adds diethyl ether, ice-bath ultrasonic was extracted 15 minutes, filtered, and filtrate evaporate to dryness, residue add chloroform 10ml makes dissolving, as need testing solution A.Filter residue dries, and puts in the lump in the 50ml band plug erlenmeyer flask together with filter paper, adds 95% ethanol 20ml, and ultrasonic Extraction 20 minutes filters, and filtrate evaporate to dryness, residue add ethanol 2ml makes dissolving, as need testing solution B.
(2) chemical method is differentiated
1. get need testing solution B 0.5ml, evaporate to dryness adds 1 of 1 of the concentrated sulfuric acid and vanillin, shows purple.
2. get need testing solution B 1ml, add a little magnesium powder jolting, add 2 of concentrated hydrochloric acids, placed 15 minutes, show peony.
3. get need testing solution B point on filter paper, dry, put under the ultraviolet lamp (365nm) and observe, show pistac.Behind the ammonia cure, spray test solution again, dry, put under the ultraviolet lamp (365nm) and observe, show yellow green with 1%AlCl3.
(3) ultraviolet spectrophotometry is differentiated
1. get need testing solution A 1ml, be diluted with water to 100ml, shake up,, maximum absorption band is arranged, see Figure 25 at the wavelength place of 256nm, 295nm according to spectrophotometry.
2. get need testing solution B 0.1ml, evaporate to dryness, residue adds the 0.1%NaOH dissolve with ethanol solution and is transferred in the 50ml measuring bottle, add the 0.1%NaOH ethanolic solution and be diluted to scale, shake up, according to spectrophotometry, at the wavelength place of 220nm, 235nm, 250nm, 292nm maximum absorption band is arranged, see Figure 26.
(4) thin-layer chromatography
Test according to thin-layer chromatography (an appendix VI of Chinese Pharmacopoeia version in 2005 B), need testing solution A 4 μ l are got in absorption, put on silica gel g thin-layer plate, and be solvent with n-hexane-ethyl acetate (85: 15), launch 9cm, take out, dry, spray is with 10% phosphomolybdic acid ethanol solution, it is clear to be heated to spot colour developing at 105 ℃, put under the daylight and inspect, show black-and-blue spot, see Figure 27.
3. the discriminating of galanga galangal rhizome and adulterant
(1) former phytomorph is differentiated
The former phytomorph of galanga galangal rhizome and adulterant is differentiated and is seen Table 3.
The phytomorph of table 3. galanga galangal rhizome and adulterant is differentiated
| Kind | Phytomorph |
| | 1. plant is taller and bigger, the rhizome diameter reaches 2.5cm, leaf length is more than 30cm, fruit dolioform or spherical 2. large-scale panicles, spend little, white ghost, fruit is dolioform, redness when |
| Galangal | |
| 1. less, the rhizome diameter of plant is below 1.5cm, leaf length is below 30cm, long 1～the 3cm of non-dolioform 2. tips of a leaf of fruit or more than, long 2～the 3cm of raceme 3. tips of a leaf or more than, do not ftracture, fruit is spherical | |
| Intelligence development | 3. the long 1～2cm of the tip of a leaf ftractures, and fruit is oval |
| In one's early teens | 2. raceme is upright, spend greatly, and beauty, fruit is spherical, and is orange-yellow when ripe |
(2) proterties is differentiated and is seen Table 4.
Table 4. galanga galangal rhizome and adulterant proterties are differentiated
| Kind | N Branch/ fork | d Diameter/cm | N Skin zone∶N Center pillar | Smell | Quality |
| | 0～2 | 2.0～2.5 | 1∶1 | Light | A little less than the oiliness |
| Galangal | 2～3 | 1.0～1.5 | 3∶2 | Fragrant peppery | Oiliness is strong |
| Intelligence development | Cross, 3 | 0.6～1.0 | 2∶3 | Light | Fibroid |
| Apart from the flower Alpinia japonica | 2～3 | 0.7～0.8 | 3∶2 | Light | In the oiliness |
| In one's early teens | 2～3 | 1.5～1.8 | 2∶3 | Light | Fibroid |
(3) microscopic features are differentiated and are seen Table 5.
Table 5. galanga galangal rhizome and adulterant microscopic features are differentiated
| Kind | N The vascular bundle inner catheter/ | N Bundle nitre fiber/ row | Eleocyte | The starch grain shape | The fiber pit |
| Galanga galangal rhizome | 2～7 | 2～4 | Few | Oval, long ovum | |
| Galangal | |||||
| 3,5,9, be many with 10 | 2～3 | Many | Rice, bottle, ovum, oval | Significantly, beaded | |
| | 1,3,5, be many with 7 | 3,5,7, be many with 7 | Few | Oval, ovum | Not obvious |
| Apart from the flower Alpinia japonica | With 1,2 is many, and 3,4,5 for few | 2、3、4 | Few | Oval, ovum | Not obvious |
| In one's early teens | 2～7 | 2～5 | Few | Ovum, oval | Not obvious |
(4) physics and chemistry is differentiated and is seen Table 6.
1. volatile oil chromogenic reaction: the ether extracted liquid of each sample adds the concentrated sulfuric acid and vanillin, and chromogenic reaction is promptly arranged.
2. flavonoids fluorescence reaction: the alcohol extract drop of each sample drips the alchlor test solution on filter paper, be under the ultraviolet light of 365nm at wavelength, has or do not have the fluorescence reaction of flavonoids.
The physics and chemistry of table 6. galanga galangal rhizome and adulterant is differentiated
| Kind | The volatile oil chromogenic reaction | The flavonoids fluorescence reaction |
| Galanga galangal rhizome | Puce | No fluorescence |
| Galangal | Rose | Aquamarine fluorescence |
| Intelligence development | Aubergine | Light green fluorescence |
| Apart from the flower Alpinia japonica | Brownish red | Yellow-greenish phosphorescent light |
| In one's early teens | Brownish red | Light green fluorescence |
| Blank (sulfuric acid, vanillin) | Light yellow | |
| Blank (alchlor test solution) | No fluorescence |
(5) ultraviolet spectra is differentiated
Take by weighing galanga galangal rhizome, galangal, intelligence development, each 0.5g of distance flower Alpinia japonica powder respectively, the 5ml that adds diethyl ether floods 4h, filters.Filtrate is made into the solution of 3mg/ml with ether, to be blank with batch solvent, on ultraviolet specrophotometer, in the interscan of 200～400nm wave-length coverage, the results are shown in Table 7.
Table 7. galanga galangal rhizome and adulterant ultraviolet spectra are differentiated
| Kind | Characteristic ultraviolet absorption |
| Galanga galangal rhizome | λ Et 2O is 219nm; There is acromion at 261nm wavelength place.Max |
| Galangal | λ Et 2O is 219,265,287,306,357nm.Max |
| Intelligence development | λ Et 2O is 262nm; There is acromion at the 306nm place.Max |
| Apart from the flower Alpinia japonica | λ Et 2O is 219,268,360nm; 289, there is acromion at 306nm wavelength place.max |
(6) the gas-chromatography finger-print is differentiated
The quality that single chemical composition can not the thoroughly evaluating galanga galangal rhizome is differentiated in galanga galangal rhizome and adulterant galangal, intelligence development, in one's early teens, all contain volatile oil apart from the rhizome of flower Alpinia japonica, therefore sets up the gas-chromatography finger-print and differentiates that the authenticity of galanga galangal rhizome is extremely important.Method is as follows:
The preparation of need testing solution: get each 20g of galanga galangal rhizome and adulterant, press an appendix X of Chinese Pharmacopoeia version in 2005 D determination of volatile oil Division A League Matches of French Football method and extract volatile oil, the volatile oil of extraction is with 20 times of ethyl acetate dilutions, as need testing solution.
Chromatographic condition: chromatographic column: Supelco Simplicty-wa * 330m * 320 μ m * 0.25 μ m; Column temperature: carrier gas: N
21mL/min; Split ratio: 50: 1; Vaporizer temperature: 250 ℃; Detector temperature: 280 ℃; Sample size: 1 μ L.
Measure and write down the gas chromatogram of galanga galangal rhizome and each adulterant sample volatile oil according to above-mentioned condition.
As a result, the gas-chromatography finger-print of various types of volatile oil has characteristic peak separately respectively, can differentiate galanga galangal rhizome effectively by this characteristic peak, sees Figure 28.
(3) check
1, impurity
Check according to an appendix IX of Chinese Pharmacopoeia version in 2005 A determination of foreign matter method.
2, moisture
Measure according to an appendix IX of Chinese Pharmacopoeia version in 2005 H aquametry.
3, ash content
Measure according to an appendix IX of Chinese Pharmacopoeia version in 2005 K ash determination method.
4, persticide residue
Measure according to an appendix IX of Chinese Pharmacopoeia version in 2005 Q persticide residue determination method.
5, heavy metal
Check according to an appendix IX of Chinese Pharmacopoeia version in 2005 E heavy metal method of control.
6, arsenic salt
Check according to an appendix IX of Chinese Pharmacopoeia version in 2005 F arsenic salt method of control.
(4) assay
According to the content of 1 '-acetoxy-chavicol-acetate in high performance liquid chromatography (an appendix VI of Chinese Pharmacopoeia version in 2005 D) the mensuration galanga galangal rhizome, see Figure 29.
Chromatographic condition and system suitability test are filler with the octadecylsilane chemically bonded silica; With methanol-water (50: 50) is flowing phase; The detection wavelength is 254nm.Number of theoretical plate calculates by 1 '-acetoxy-chavicol-acetate peak should be not less than 3000.
It is an amount of that the preparation precision of reference substance solution takes by weighing the contrast of 1 '-acetoxy-chavicol-acetate, adds methyl alcohol and make the solution that every 1ml contains 20 μ g, promptly.
The about 1g of galanga galangal rhizome powder (crossing sieve No. 3) is got in the preparation of need testing solution, the accurate title, decide, and puts in the apparatus,Soxhlet's, and it is an amount of to add ethanol, heating and refluxing extraction 2 hours, collect extract, divide three extractions, combining extraction liquid with n-hexane-ethyl acetate (10: 1) solution of equivalent, the recovered under reduced pressure extractant, extract is eluent wash-out purifying with silica gel column chromatography (3cm * 50cm chromatographic column separates, 20 times of amount silica gel) with n-hexane-ethyl acetate (10: 1), collects eluent, water bath method, residue is put in the 10ml measuring bottle, and it is an amount of to add methyl alcohol, ultrasonicly makes dissolving, add methyl alcohol to scale, shake up, filter, get subsequent filtrate as need testing solution.
Determination method: precision is got reference substance solution and each 20 μ l of need testing solution respectively, injects liquid chromatograph, and the record chromatogram is by the content of external standard method with calculated by peak area 1 '-acetoxy-chavicol-acetate, promptly.
Eight, galanga galangal rhizome packed and transported and storage
(1) packing
After galanga galangal rhizome dries, pack with special-purpose bag.According to the requirement of " traditional Chinese medicine quality of production management regulation ", should spot-check, remove inferior goods and impurity once more before packing, the packing equipment should be pollution-free, clean clean, dry, nothing breakage; In the packaging bag packing record should be arranged, content should comprise: the name of an article, lot number, specification, weight, the place of production, job number, date etc.
(2) transportation
During the galanga galangal rhizome bulk transport, attention can not load in mixture by poisonous with other, harmful material; Prevent the moisture absorption, prevent to tan by the sun.
(3) storage
1, holding conditions
Because of galanga galangal rhizome contains volatile oil, should deposit in the MED DEP of cool place, drying, and prevent moisture regain, anti-damaging by worms.Storage temperature is below 30 ℃, and relative moisture is controlled at 70%～75%, and commercial articles safety moisture is 10%～13%.According to the provisions of the relevant regulations issued by the State, the proportion of goods damageds three months, six months, the storehouse loss more than a year and a year are respectively in 1%, 1.3%, 1.6%, 2% by norm to store keeping.
2, anti-damaging by worms
But the fragrant pungent smell insect protected of galanga galangal rhizome so the phenomenon of damaging by worms is less, but is still easily damaged by worms in the wet environment.
Common stored grain insects have Stegobium paniceum, lasioderma serricorne, Lyctus brunneus Stephens, black wool khapra beetle, dun clerid etc., are eaten into the visible channel in medicinal material surface of erosion, the outer brownish red now of packing moth end.Therefore, should keep warehouse drying, cleaning, often dust out, foreign material regularly carry out disinfection.Discovery is damaged by worms, and should move airing outward immediately, turn over the buttress ventilation.The warehouse is airtight, and vacuumizing is filled out back nitrogen and is preserved.
3, anti-making moist gone mouldy
In wet environment, the galanga galangal rhizome moisture-sensitive is gone mouldy.As find medicinal material in wet condition, can tan by the sun in the sun, or water cleans mould, after tan by the sun.But should not often tan by the sun, in order to avoid volatile oil is lost, surperficial drying shrinkage, the color and luster dimness influences its quality.Should check 1 time every 15 days season at intermittent drizzles in the rainy season, pinpoint the problems and in time handle, guarantee the outside and the inherent quality of galanga galangal rhizome.
The effect that obtains
Adopt the galanga galangal rhizome of the inventive method standardized cultivation to see Figure 30.
1. the seedling breeding effect sees Table 8.
Table 8. seedling breeding effect
| The seedling breeding method | The seedling breeding effect |
| The rhizome breeding | Adaptability to environment is strong, the thick length soon of giving birth to, and the plant division ability is strong, portable after month, economical and effective. |
| Seminal propagation | Growth of seedling speed is fast, yields poorly, and is of poor quality, ability portable after the half a year of growing seedlings. |
| Tissue culture | Be not subject to seasonal restrictions, realize large-scale industrialized fast seedling-breeding, just portable after one week, seedling is removed diseases such as virus, fungi, bacterium, growth is fast, cycle is short, repeatability is strong, the economic benefit height. |
2. quality testing result
(1) proterties: adopt the galanga galangal rhizome of the inventive method cultivation consistent with the proterties of wild galanga galangal rhizome.
(2) micro-discriminating: adopt the galanga galangal rhizome of the inventive method cultivation consistent with the micro-identification result of wild galanga galangal rhizome.
(3) chemistry is differentiated: adopt the galanga galangal rhizome of the inventive method cultivation and wild galanga galangal rhizome need testing solution chemistry differential method all to be positive.
(4) ultraviolet spectra is differentiated: adopt the galanga galangal rhizome of the inventive method cultivation in the same wave strong point identical maximum absorption band to be arranged all with the ether extract and 95% ethanol extract of wild galanga galangal rhizome.
(5) thin layer chromatography is differentiated: adopt the galanga galangal rhizome of the inventive method cultivation and wild galanga galangal rhizome need testing solution all shows same color in same position spot.
(6) the gas-chromatography finger-print is differentiated: the galanga galangal rhizome of employing the inventive method cultivation and the extractive of volatile oil of wild galanga galangal rhizome show the gas-chromatography fingerprint peaks of same characteristic features.
(7) adopt the galanga galangal rhizome of the inventive method cultivation and impurity, moisture, ash content, persticide residue, heavy metal, the arsenic salt check result no significant difference of wild galanga galangal rhizome.
(8) content: adopt in the galanga galangal rhizome of the inventive method cultivation 1 '-extract yield of acetoxy-chavicol-acetate is 1.2%～2.8%, purity is 82%～96%; The extract yield of wild galanga galangal rhizome is 1.5%～2.7%, and purity is 85%～91%.
3. pharmacological component
Adopt galanga galangal rhizome and wild galanga galangal rhizome water, alcohol, ether solvents extraction and the super clinical boundary extraction of the inventive method cultivation all to obtain following composition, see Table 9 with pharmacologically active.
Table 9. galanga galangal rhizome extract active component table
| Sequence number | The active component title | Molecular formula | Molecular weight | Physical constant | Pharmacologically active |
| 1 | 1 '-acetoxy-chavicol-acetate (1 '-Acetoxy-chavicol acetate) | C 13H 14O 4 | 234.25 | [α] D 20=-80 ° (C=1, ethanol).IRγmax(liq)cm -1： 1760，1740，1600，1500， 1200～1240。MSm/z：234(M +)， 192，150，133，132，131。 | 1. antitumor: mouse S
18010mg/kg d, the 2. antimycotic 3. antiulcer of |
| 2 | 1 '-the acetoxyl group NSC 1242 (1 '-Acetoxy-eugenol acetate) | C 14H 16O 5 | 264.28 | IR γ max (liq) cm -1: 1760,1740,1600,1500,1200～1240.MSm/z:264 (M +), 222,180,163,162,133,132,131 | 1. antitumor: mouse S
180, 10mg/kg d, growth rate 10.0%.2. antiulcer: rat ip, stomach ulcer, 5mg/kg, inhibiting rate 36%, 10mg/kg, inhibiting |
| 3 | (E)-8 β (17)-Epoxylabd-12-ene-15,16-dial (Aframodial) bacterium, MIC=12.5 μ g/ml. | G 20H 30O 3 | 318.46 | Colourless acicular crystal (methyl alcohol-water), mp:90～92 ℃, [α] D=+27.3 ° (C=0.27, chloroform). | 1. lipidemia: mouse, rat liver homogenate, it is synthetic to suppress cholesterol.2. antibiotic: anti-gram-negative bacteria, gram positive bacteria, golden yellow Penicillium notatum.3. antimycotic: white beads |
| 4 | Camphor (Camphor) | C 10H 16O | 152.24 | Rhomboid crystallization (ethanol), mp:(+) 179.75 ℃, (-) 178.6 ℃.bp：(+)204℃， (-)204℃。[α] D 25=41 °～43 ° (ethanol) | 1. cardiotonic.2. local irritant. |
| 5 | Eugenol (Eugenol) | C 10H 12O 2 | 164.21 | Mp:-9 ℃.Bp:254～255 | 1. antibacterial agent.2. antimycotic.3. antihistamine.4. anti-oxidant.5. spasmolysis.6. central nervous system there is remarkable effect.7. platelet aggregation-against.8. analgesic. |
| 6 | Galangal terpene aldehyde A (Galanal A) | C 20H 30O 3 | 318.46 | Colourless diamond crystal.mp：167～169℃。[α] D=-44 v(C=0.1, chloroform). | Antimycotic: candida guilliermondi, MIC=12.5 μ g/ml. |
| 7 | Galangal terpene aldehyde B (Galanal B) | C 20H 30O 3 | 318.46 | Colourless diamond crystal.mp：134.0～134.5℃。[α] D=-48 v(C=0.1, chloroform). | Antimycotic: candida guilliermondi, MIC=12.5 μ g/ml, Candida tropicalis, MIC=50 μ g/ml. |
| 8 | Galangin (Galagin) | C 15H 10O 5 | 270.24 | Yellow acicular crystal (methyl alcohol).mp：214～216℃。 | 1. antimicrobial.2. cox-2 inhibitors.3. mutagen: salmonella typhimurium TA98 and TA100. |
| 9 | Galanga Galangal Seed lactone (Galanolactone) | C 20H 30O 3 | 318.46 | Colourless needle (methanol-water).mp：125.5～126.0℃。[α] D=+28.0 v(C=0.26, chloroform). | 1. antimycotic: the MIC to candida guilliermondi and Candida tropicalis is 25 μ g/ml.2. anti-5-HT effect. |
| 10 | Methyl cinnamate (Methylcinnamate) | G 10H 10O 2 | 162.19 | Mp:36.5 ℃.bp：261℃/99.99KPa。 | 1. phlegm-dispelling functions: the bronchial gland secretion is increased.2. antiprotozoan effect. |
The Figure of description explanation
Accompanying drawing 1 is galanga galangal rhizome Alpinia galanga willd.
Accompanying drawing 2 is the galanga galangal rhizome plant
Accompanying drawing 3 is the galanga galangal rhizome flower
Accompanying drawing 4 is the galanga galangal rhizome rhizome
Accompanying drawing 5 is the galanga galangal rhizome panicle
Accompanying drawing 6 is the galanga galangal rhizome fruit
Accompanying drawing 7 is the galanga galangal rhizome tip of a leaf
The wild galanga galangal rhizome of accompanying drawing 8 in thick grass, growing
Accompanying drawing 9 is the wild galanga galangal rhizome in the shady wet sylvan life growth of hill cheuch
Accompanying drawing 10 is a galanga galangal rhizome root stem rot
Accompanying drawing 11 is cross section epidermis of galanga galangal rhizome rhizome and cortical cell
Accompanying drawing 12 is the cross section root trace bundle of galanga galangal rhizome rhizome
Accompanying drawing 13 is the cross section cortex secretory cell of galanga galangal rhizome rhizome
Accompanying drawing 14 is cross section cortical bundle of galanga galangal rhizome rhizome and fiber bundle-sheath
Accompanying drawing 15 is cross section endodermis of galanga galangal rhizome rhizome and center pillar vascular bundle
Accompanying drawing 16 is cross section center pillar vascular bundle of galanga galangal rhizome rhizome and fiber bundle-sheath
Accompanying drawing 17 is the starch grain of galanga galangal rhizome powder
Accompanying drawing 18 is the fiber bundle of galanga galangal rhizome powder
Accompanying drawing 19 is that the fiber side of galanga galangal rhizome powder is seen
Accompanying drawing 20 is for being the parenchyma cell of galanga galangal rhizome powder
Accompanying drawing 21 is the secretory cell of galanga galangal rhizome powder
Accompanying drawing 22 is the epidermal cell of galanga galangal rhizome powder
Accompanying drawing 23 is the scalariform duct of galanga galangal rhizome powder
Accompanying drawing 24 is a galanga galangal rhizome powder reticulate vessel
Accompanying drawing 25 is a galanga galangal rhizome ether extract ultra-violet absorption spectrum
Accompanying drawing 26 is galanga galangal rhizome 95% an ethanol extract ultra-violet absorption spectrum
Accompanying drawing 27 is a galanga galangal rhizome ether extract thin-layer chromatogram
Accompanying drawing 28 is a galanga galangal rhizome gas-chromatography finger-print
Accompanying drawing 29 is 1 '-acetoxy-chavicol acetate HPLC figure in the galanga galangal rhizome
Accompanying drawing 30 is for adopting the galanga galangal rhizome of the inventive method standardized cultivation
Claims (13)
1. the culture of alpinia galanga method is characterized in that it is seedling breeding, transplant planting, field management, fertilising, the extermination of disease and insect pest, the application of the process technology of gathering in the rhizome culture of alpinia galanga of Zingiber Zingiberaceae Alpinia plants Galanga Galangal Seed Alpiniagalanga Willd..
2. the described culture of alpinia galanga method of claim 1 is characterized in that it is to adopt rhizome, seminal propagation technology to carry out the method for galanga galangal rhizome seedling breeding.
3. the described culture of alpinia galanga method of claim 1, it is characterized in that it is to adopt tissue culture technique, with fresh galanga galangal rhizome is explant, be seeded in after the sterilization carry out in the medium young shoot, the clump bud induce the method for carrying out the galanga galangal rhizome seedling breeding with the root induction technology.
4. the described culture of alpinia galanga method of claim 1, the transplant planting that it is characterized in that it is at the torrid zone, fine morning or the overcast and rainy transplant planting that carry out galanga galangal rhizome seedling of subtropical climate between regional 3～April, require deep, loose, fertile acidity or the subacidity sand loam of soil layer, the planting density seeding row spacing is 100 * 100 centimetres, open the cave by seeding row spacing on whole good furrow, the specification in cave is 40 * 40 * 30 centimetres; When the seed height of seedling is more than 20 centimetres, go out the garden field planting, every cave kind 2～3 strain seedling, or 1 root-like stock of every cave kind, bud head-up bankets while putting, and plants back earthing compacting, and then covers the galanga galangal rhizome seedling transplant planting method of the fine earth of 5～6 cm thicks.
5. the described culture of alpinia galanga method of claim 1 is characterized in that it is weeding in every month 1 time when there be not the envelope row in early stage, and each weeding of Feng Hanghou autumn in summer 1 time is loosened the soil during weeding in passing; Plantation back planted on every side in strain on the 2nd year that trench digging loosens the soil, and earthed up simultaneously; Or stop up at the plant base portion with farmyard manure and table earth culture in conjunction with clear garden in the time of the year when autumn changes into winter, keep ground moistening, promote the culture of alpinia galanga field management method of plant tillering and root growth.
6. the described culture of alpinia galanga method of claim 1, the fertilising that it is characterized in that it is the nutrient demand characteristics according to soil fertility situation and galanga galangal rhizome different growing stage, and does not pollute plant, soil and galanga galangal rhizome content of beary metal are exceeded standard impose the culture of alpinia galanga fertilizing method of calcium, magnesium, organic matter, trace element, nitrogen, potassium, phosphate fertilizer for principle.
7. the described culture of alpinia galanga method of claim 1, the pest control method that it is characterized in that it are to irritate root control root stem rot with 0.2～0.4 Baume Lime Sulphur; The pest control method is to prevent and treat flat snail with 6% carbaryl-methaldehyde.
8. the described culture of alpinia galanga method of claim 1, it is characterized in that it is to gather in 2～March, the rhizome of gathering is removed acrial part, earth, fibrous root and scale, and be cut into 5～6 centimetres segment, clean, method that the galanga galangal rhizome medicinal material is made in segment, drying and be lower than the method that 30 ℃, relative moisture are storage galanga galangal rhizome medicinal material under 70%～75% the condition in temperature, the moisture of control galanga galangal rhizome medicinal material is 10%～13%.
9. the galanga galangal rhizome method of quality control is characterized in that it is application and the high performance liquid chromatography application in active component 1 '-acetoxy-chavicol-acetate content in measuring galanga galangal rhizome in the qualitative discriminating of galanga galangal rhizome of uv-spectrophotometric, thin-layer chromatography, gas-chromatography finger print method.
10. the described galanga galangal rhizome method of quality control of claim 9, the qualitative discrimination method of ultraviolet spectrophotometry that it is characterized in that it is that the galanga galangal rhizome ether extract has maximum absorption band at the wavelength place of 256nm and 295nm; Galanga galangal rhizome 95% ethanol extract has the ultraviolet spectra discrimination method of maximum absorption band at the wavelength place of 220nm, 235nm, 250nm, 292nm.
11. the described galanga galangal rhizome method of quality control of claim 9, the qualitative discrimination method of thin layer chromatography that it is characterized in that it is that galanga galangal rhizome ethanol extract solution point is on silica gel g thin-layer plate, with n-hexane-ethyl acetate is solvent, launch, take out, dry, spray is with 10% phosphomolybdic acid ethanol solution, it is clear to be heated to spot colour developing at 105 ℃, puts under the daylight and inspects, and shows the thin layer chromatography discrimination method of blue spot.
12. the described galanga galangal rhizome method of quality control of claim 9, the gas-chromatography fingerprint discrimination method that it is characterized in that it are with Supelco Simplicity-wax 30m * 320 μ m * 0.25 μ m chromatographic column; 80 ℃～230 ℃ of column temperatures, column temperature programming rate are 10 ℃ of per minutes; Carrier gas: N
21ml/min; Split ratio is 50: 1; The vaporizer temperature is 250 ℃; Detector temperature is 280 ℃; Get the galanga galangal rhizome extractive of volatile oil, with 20 times of ethyl acetate dilutions, sample introduction 1 μ l, record gas chromatogram, the gas-chromatography fingerprint discrimination method at detected characteristics peak.
13. the described galanga galangal rhizome method of quality control of claim 9, it is characterized in that it is the method that adopts composition 1 '-acetoxy-chavicol-acetate content in the high effective liquid chromatography for measuring galanga galangal rhizome: with the octadecylsilane chemically bonded silica is filler; With the methanol-water is flowing phase; The detection wavelength is 254nm.The methanol solution that contains 20 μ g, 1 '-acetoxy-chavicol-acetate reference substance with every 1ml is a reference substance solution; Galanga galangal rhizome is extracted with alcohol heating reflux, extract n-hexane-ethyl acetate extraction, extract separates with silica gel column chromatography, with n-hexane-eluent ethyl acetate purifying, collect the wash-out refined solution, water bath method, residue adds methyl alcohol, ultrasonicly make dissolving, filter, get subsequent filtrate as need testing solution.Precision is got reference substance solution and each 20 μ l of need testing solution respectively, injects liquid chromatograph, and the record chromatogram is by the method for external standard method with composition 1 ' in the calculated by peak area galanga galangal rhizome-acetoxy-chavicol-acetate content.
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