CN103858767A - Alpinia henryi high-yield cultivation method - Google Patents
Alpinia henryi high-yield cultivation method Download PDFInfo
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- CN103858767A CN103858767A CN201410117533.8A CN201410117533A CN103858767A CN 103858767 A CN103858767 A CN 103858767A CN 201410117533 A CN201410117533 A CN 201410117533A CN 103858767 A CN103858767 A CN 103858767A
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Abstract
The invention relates to an alpinia henryi high-yield cultivation method. The method comprises the following steps: tissue culture for seedling growth: collecting seeds after 7-8 continuous sunshine days, digging out an alpinia henryi plant used as a seed from the soil in a clump manner 2-3 days before seed production, removing mud and hanging upside down for airing for 2-3 days in an indoor air-ventilating and shady place; hardening seedling in a shed: removing a shed plastic film when 5-6 leaves grow, hardening the seedling for 5-10 days to improve the external environment adaptive ability, and at the moment, shading is still required; transplanting into a nutrition pot: picking the seedling from a seedling growing furrow after the seedling is 15-20cm in height, and transplanting into the nutrition pot made of naturally degraded material; and planting in the field.
Description
Technical field
The present invention relates to a kind of cultivation method, particularly relate to a kind of little Amomum globosum loureiro high-yield cultivating method.
Background technology
Little Amomum globosum loureiro, Zingiber, Alpinia plants, blade wire lanceolar, top is point tool small cusp gradually, and base portion is gradually narrow, and except edge is by hair, two sides is all without hair; The tip of a leaf is blunt, keratin.Raceme is upright, and rhachis is by thin,tough silk hair; Bractlet Long Circle, calyx mitriform, corolla is without hair, and style is without hair; Ovary spheroidal, fruit spheroidal, florescence: the 4-6 month; The fruit phase: the 5-7 month.Produce China Guangdong, be born in thick forest, also there is distribution in Vietnam.The cultivation method of conventional ginger is that mid or late March starts to shine ginger, tired ginger, indoor vernalization, about about 20-30 days Furrow sowings in the time that bud grows to 1-1.5cm.Because little Amomum globosum loureiro germinates slowly early stage, to responsive to temperature, before sowing, need that time is long, main threads is loaded down with trivial details, the effort of taking a lot of work, need 25-30 days from being seeded into emerge, need 25-30 days from emerging to 5-6 sheet leaf, cause during this time idle land for up to 60-70 days, be enough to plant one batch of spring vegetables.And ginger seedling fearness high light, need field scaffolding to shade.Conventional method plantation ginger is time-consuming, effort, plants little careless guan usage amount large, and the field land occupation time is long, within 1 year, can only plant a season, is unfavorable for crops for rotation adjusting, and seedling-growing time is inconsistent, causes large seedling, weak seedling, is short of seedling.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of little Amomum globosum loureiro high-yield cultivating method, comprises the steps:
Tissue culture: collect seed and be chosen in continuous sunny and carry out after 7～8 days, before the production of hybrid seeds 2～3 days from soil Cheng Cong dig out the little Amomum globosum loureiro plant that needs to do kind, remove earth, and hanging upside down and drying 2～3 days in the place in room ventilation cool place; The tender shoots of the lateral bud on selection stem tuber or pumping is as explant material; Remove after blade and root system long on stem tuber, under running water, rinse earth well, and the tender shoots as explant material and old stem are cut open, and dab as the tender shoots of explant material and the surface of stem tuber with 0.5% the washing powder water that wet degreasing cotton dips in by weight, then under running water, rinse 10～20 minutes; On clean bench, remove as unnecessary blade and subtending leaf on the tender shoots of explant material; To on stem tuber, cut as the large lateral bud in the lateral bud of explant material, every explant material size is 1～2cm × 1～2cm size, every bottle of 3～4 block of material; With after 75% alcohol-pickled 30s, with aseptic water washing once, 0.1% mercuric chloride solution that reinjects carries out soaking disinfection 20～25 minutes, rinsing 5～6 times in sterile water, each 5～6 minutes; For subsequent use in rear immersion sterile water; To proceed on the induced bud medium just forming for induced bud every bottle of 1 bud, the wide-mouth vial that culture vessel is 250ml through the explant material of above-mentioned processing; Described induced bud medium is: MS+6-BA4.0～5.0mgL
-1+ KT5.0mgL
-1+ NaH
2pO
4170mgL
-1+ white sugar 3%+ agar 0.6%, pH5.8～6.0; 6-BA is 6-benzyl aminopurine; KT is 6-Furfurylaminopurine; Condition of culture is: intensity of illumination 12.5～19 μ molm
-2s
-1, 27～28 DEG C of temperature, cultivation cycle 30～40 days, at this moment takes out explant material, carries out cutting process on clean bench; Cutting method is as follows: the tissue block of brownization on excision material, remove the unnecessary blade of sprout, and keep the agglomerate size of 1～2cm; Then proceed in medium of the same race, successive propagation 3～4 times, cultivation cycle is 30～40 days until grow 2～4 indefinite buds as the base portion of agglomerate of cultivating body, in the time that indefinite bud growth and propagation stablize, proceed to and in proliferated culture medium, breed the generation of cultivation evoking adventive bud; Adventitious bud proliferation is cultivated the agglomerate that grows indefinite bud is bred to cutting by the direction of growth, and each agglomerate retains 2～3 indefinite buds, and excises long blade, in access proliferated culture medium; Adventitious bud rooting is cultivated, and cuts that growth is normal, blade face band is pinto, the solid bud of high 2～3cm from the bud clump of described indefinite bud, is inoculated on root media; The cultivation agglomerate that does not reach the standard of taking root is bred cutting by the direction of growth, and each agglomerate retains 2～3 indefinite buds, and agglomerate bud is put into described proliferated culture medium and breed cultivation; When described indefinite bud is that seedling was grown 20～35 days time on root media, starting has rootlet to grow; Now by high 3～5cm, carry out domesticating and cultivating with the plantlet of transplant up to standard of 2～4 leaves and tool root in green house; When transplanting, clean the medium being attached on root, to reduce the leaf phenomenon of being completely cured; And with 800 times of tpns or take turns many grams and soak after 5 minutes and plant on screen tray, training matrix is peat soil and perlite mixed-matrix, ratio 3: 1;
Hardening in canopy: in the time that seedling grows to 5-6 sheet leaf, open canopy film, hardening 5-10 days, the ability that adapts to external environment to improve it, now still needs to shade;
Nutritive cube is transplanted: the seedling of height of seedling 15-20cm is proposed in the furrow of growing seedlings, be transplanted to by the nutritive cube of can natural degradation material making;
Field field planting: line-spacing 60-65cm ridging is pressed in field, the high 10-20cm in ridge, overlay film after spraying herbicide; On mulch film, beat the implantation hole of nutritive cube size, nutrition pot culture is entered in the inter-drain of ridge, water after suitable quantity of water, earthing lid is real, and holding temperature is 20～30 DEG C, after transplanting summer, covers two weeks with film, winter, film covered three weeks, and every day, spraying was processed, and controlled humidity is 85～95%; Sooner or later open film bottom and two ends, be beneficial to ventilated; In the time that thin temperature in the shed exceedes 32 DEG C, also open film bottom and two ends, to reduce temperature and ventilation noon in summer; Transplant in latter 1 month, controlled light intensity is at 50 μ molm
-2s
-1below, after growth is stable, intensity of illumination is increased to 100～125 μ molm
-2s
-1.；
Embodiment
A kind of little Amomum globosum loureiro high-yield cultivating method comprise grow seedlings, vernalization is taken root, hardening, nutritive cube are transplanted in canopy, the operation such as field field planting and field management and results forms; Comprise the steps:
(1) tissue culture: collect seed and be chosen in continuous sunny and carry out after 7～8 days, before the production of hybrid seeds 2～3 days from soil Cheng Cong dig out the little Amomum globosum loureiro plant that needs to do kind, remove earth, and hanging upside down and drying 2～3 days in the place in room ventilation cool place; The tender shoots of the lateral bud on selection stem tuber or pumping is as explant material; Remove after blade and root system long on stem tuber, under running water, rinse earth well, and the tender shoots as explant material and old stem are cut open, and dab as the tender shoots of explant material and the surface of stem tuber with 0.5% the washing powder water that wet degreasing cotton dips in by weight, then under running water, rinse 10～20 minutes; On clean bench, remove as unnecessary blade and subtending leaf on the tender shoots of explant material; To on stem tuber, cut as the large lateral bud in the lateral bud of explant material, every explant material size is 1～2cm × 1～2cm size, every bottle of 3～4 block of material; With after 75% alcohol-pickled 30s, with aseptic water washing once, 0.1% mercuric chloride solution that reinjects carries out soaking disinfection 20～25 minutes, rinsing 5～6 times in sterile water, each 5～6 minutes; For subsequent use in rear immersion sterile water; To proceed on the induced bud medium just forming for induced bud every bottle of 1 bud, the wide-mouth vial that culture vessel is 250ml through the explant material of above-mentioned processing; Described induced bud medium is: MS+6-BA4.0～5.0mgL
-1+ KT5.0mgL
-1+ NaH
2pO
4170mgL
-1+ white sugar 3%+ agar 0.6%, pH5.8～6.0; 6-BA is 6-benzyl aminopurine; KT is 6-Furfurylaminopurine; Condition of culture is: intensity of illumination 12.5～19 μ molm
-2s
-1, 27～28 DEG C of temperature, cultivation cycle 30～40 days, at this moment takes out explant material, carries out cutting process on clean bench; Cutting method is as follows: the tissue block of brownization on excision material, remove the unnecessary blade of sprout, and keep the agglomerate size of 1～2cm; Then proceed in medium of the same race, successive propagation 3～4 times, cultivation cycle is 30～40 days until grow 2～4 indefinite buds as the base portion of agglomerate of cultivating body, in the time that indefinite bud growth and propagation stablize, proceed to and in proliferated culture medium, breed the generation of cultivation evoking adventive bud; Adventitious bud proliferation is cultivated the agglomerate that grows indefinite bud is bred to cutting by the direction of growth, and each agglomerate retains 2～3 indefinite buds, and excises long blade, in access proliferated culture medium; Adventitious bud rooting is cultivated, and cuts that growth is normal, blade face band is pinto, the solid bud of high 2～3cm from the bud clump of described indefinite bud, is inoculated on root media; The cultivation agglomerate that does not reach the standard of taking root is bred cutting by the direction of growth, and each agglomerate retains 2～3 indefinite buds, and agglomerate bud is put into described proliferated culture medium and breed cultivation; When described indefinite bud is that seedling was grown 20～35 days time on root media, starting has rootlet to grow; Now by high 3～5cm, carry out domesticating and cultivating with the plantlet of transplant up to standard of 2～4 leaves and tool root in green house; When transplanting, clean the medium being attached on root, to reduce the leaf phenomenon of being completely cured; And with 800 times of tpns or take turns many grams and soak after 5 minutes and plant on screen tray, training matrix is peat soil and perlite mixed-matrix, ratio 3: 1;
(2) hardening in canopy: in the time that seedling grows to 5-6 sheet leaf, open canopy film, hardening 5-10 days, the ability that adapts to external environment to improve it, now still needs to shade;
(3) nutritive cube is transplanted: the seedling of height of seedling 15-20cm is proposed in the furrow of growing seedlings, be transplanted to by the nutritive cube of can natural degradation material making;
(4) field field planting: line-spacing 60-65cm ridging is pressed in field, the high 10-20cm in ridge, overlay film after spraying herbicide; On mulch film, beat the implantation hole of nutritive cube size, nutrition pot culture is entered in the inter-drain of ridge, water after suitable quantity of water, earthing lid is real, and holding temperature is 20～30 DEG C, after transplanting summer, covers two weeks with film, winter, film covered three weeks, and every day, spraying was processed, and controlled humidity is 85～95%; Sooner or later open film bottom and two ends, be beneficial to ventilated; In the time that thin temperature in the shed exceedes 32 DEG C, also open film bottom and two ends, to reduce temperature and ventilation noon in summer; Transplant in latter 1 month, controlled light intensity is at 50 μ molm
-2s
-1below, after growth is stable, intensity of illumination is increased to 100～125 μ molm
-2s
-1.
Claims (1)
1. a little Amomum globosum loureiro high-yield cultivating method, comprises the steps:
(1) tissue culture: collect seed and be chosen in continuous sunny and carry out after 7～8 days, before the production of hybrid seeds 2～3 days from soil Cheng Cong dig out the little Amomum globosum loureiro plant that needs to do kind, remove earth, and hanging upside down and drying 2～3 days in the place in room ventilation cool place; The tender shoots of the lateral bud on selection stem tuber or pumping is as explant material; Remove after blade and root system long on stem tuber, under running water, rinse earth well, and the tender shoots as explant material and old stem are cut open, and dab as the tender shoots of explant material and the surface of stem tuber with 0.5% the washing powder water that wet degreasing cotton dips in by weight, then under running water, rinse 10～20 minutes; On clean bench, remove as unnecessary blade and subtending leaf on the tender shoots of explant material; To on stem tuber, cut as the large lateral bud in the lateral bud of explant material, every explant material size is 1～2cm × 1～2cm size, every bottle of 3～4 block of material; With after 75% alcohol-pickled 30s, with aseptic water washing once, 0.1% mercuric chloride solution that reinjects carries out soaking disinfection 20～25 minutes, rinsing 5～6 times in sterile water, each 5～6 minutes; For subsequent use in rear immersion sterile water; To proceed on the induced bud medium just forming for induced bud every bottle of 1 bud, the wide-mouth vial that culture vessel is 250ml through the explant material of above-mentioned processing; Described induced bud medium is: MS+6-BA4.0～5.0mgL
-1+ KT5.0mgL
-1+ NaH
2pO
4170mgL
-1+ white sugar 3%+ agar 0.6%, pH5.8～6.0; 6-BA is 6-benzyl aminopurine; KT is 6-Furfurylaminopurine; Condition of culture is: intensity of illumination 12.5～19 μ molm
-2s
-1, 27～28 DEG C of temperature, cultivation cycle 30～40 days, at this moment takes out explant material, carries out cutting process on clean bench; Cutting method is as follows: the tissue block of brownization on excision material, remove the unnecessary blade of sprout, and keep the agglomerate size of 1～2cm; Then proceed in medium of the same race, successive propagation 3～4 times, cultivation cycle is 30～40 days until grow 2～4 indefinite buds as the base portion of agglomerate of cultivating body, in the time that indefinite bud growth and propagation stablize, proceed to and in proliferated culture medium, breed the generation of cultivation evoking adventive bud; Adventitious bud proliferation is cultivated the agglomerate that grows indefinite bud is bred to cutting by the direction of growth, and each agglomerate retains 2～3 indefinite buds, and excises long blade, in access proliferated culture medium; Adventitious bud rooting is cultivated, and cuts that growth is normal, blade face band is pinto, the solid bud of high 2～3cm from the bud clump of described indefinite bud, is inoculated on root media; The cultivation agglomerate that does not reach the standard of taking root is bred cutting by the direction of growth, and each agglomerate retains 2～3 indefinite buds, and agglomerate bud is put into described proliferated culture medium and breed cultivation; When described indefinite bud is that seedling was grown 20～35 days time on root media, starting has rootlet to grow; Now by high 3～5cm, carry out domesticating and cultivating with the plantlet of transplant up to standard of 2～4 leaves and tool root in green house; When transplanting, clean the medium being attached on root, to reduce the leaf phenomenon of being completely cured; And with 800 times of tpns or take turns many grams and soak after 5 minutes and plant on screen tray, training matrix is peat soil and perlite mixed-matrix, ratio 3: 1;
(2) hardening in canopy: in the time that seedling grows to 5-6 sheet leaf, open canopy film, hardening 5-10 days, the ability that adapts to external environment to improve it, now still needs to shade;
(3) nutritive cube is transplanted: the seedling of height of seedling 15-20cm is proposed in the furrow of growing seedlings, be transplanted to by the nutritive cube of can natural degradation material making;
(4) field field planting: line-spacing 60-65cm ridging is pressed in field, the high 10-20cm in ridge, overlay film after spraying herbicide; On mulch film, beat the implantation hole of nutritive cube size, nutrition pot culture is entered in the inter-drain of ridge, water after suitable quantity of water, earthing lid is real, and holding temperature is 20～30 DEG C, after transplanting summer, covers two weeks with film, winter, film covered three weeks, and every day, spraying was processed, and controlled humidity is 85～95%; Sooner or later open film bottom and two ends, be beneficial to ventilated; In the time that thin temperature in the shed exceedes 32 DEG C, also open film bottom and two ends, to reduce temperature and ventilation noon in summer; Transplant in latter 1 month, controlled light intensity is at 50 μ molm
-2s
-1below, after growth is stable, intensity of illumination is increased to 100～125 μ molm
-2s
-1.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1947488A (en) * | 2006-11-11 | 2007-04-18 | 南昌弘益科技有限公司 | Method for culture of alpinia galanga and its quality-control |
| CN101785428A (en) * | 2009-12-01 | 2010-07-28 | 东莞市生物技术研究所 | Method for improving tissue culture reproductive speed of Alpinia zerumbet |
| CN102342215A (en) * | 2011-08-05 | 2012-02-08 | 山东省农业科学院蔬菜研究所 | Ginger seedling-raising, transplanting and cultivating method |
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2014
- 2014-03-25 CN CN201410117533.8A patent/CN103858767A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1947488A (en) * | 2006-11-11 | 2007-04-18 | 南昌弘益科技有限公司 | Method for culture of alpinia galanga and its quality-control |
| CN101785428A (en) * | 2009-12-01 | 2010-07-28 | 东莞市生物技术研究所 | Method for improving tissue culture reproductive speed of Alpinia zerumbet |
| CN102342215A (en) * | 2011-08-05 | 2012-02-08 | 山东省农业科学院蔬菜研究所 | Ginger seedling-raising, transplanting and cultivating method |
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Inventor after: Wang Xiufeng Inventor before: Zeng Jian |
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Effective date of registration: 20160413 Address after: 063021 Hebei Province, Tangshan City Kaiping District Wali Zhou Qiao village Hedong north two Street No. five Applicant after: Wang Xiufeng Address before: 518000, Shenzhen, Guangdong, Luohu District Province, guest Road, commercial building, East 1806 Applicant before: Zeng Jian |
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