CN1894581B - 检测蛋白-蛋白相互作用的方法 - Google Patents

检测蛋白-蛋白相互作用的方法 Download PDF

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Publication number
CN1894581B
CN1894581B CN2004800248641A CN200480024864A CN1894581B CN 1894581 B CN1894581 B CN 1894581B CN 2004800248641 A CN2004800248641 A CN 2004800248641A CN 200480024864 A CN200480024864 A CN 200480024864A CN 1894581 B CN1894581 B CN 1894581B
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protein
ser
glu
ala
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Chinese (zh)
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CN1894581A (zh
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K·J·李
R·阿谢尔
W·斯特拉波斯
G·巴尔内亚
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Sentikin Holdings Ltd
Columbia University in the City of New York
Life Technologies Corp
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Columbia University in the City of New York
Life Technologies Inc
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6845—Methods of identifying protein-protein interactions in protein mixtures
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1055—Protein x Protein interaction, e.g. two hybrid selection
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/94—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving narcotics or drugs or pharmaceuticals, neurotransmitters or associated receptors
    • G01N33/9406—Neurotransmitters
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/72—Assays involving receptors, cell surface antigens or cell surface determinants for hormones
    • G01N2333/726—G protein coupled receptor, e.g. TSHR-thyrotropin-receptor, LH/hCG receptor, FSH
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/10—Screening for compounds of potential therapeutic value involving cells

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • General Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)
CN2004800248641A 2003-07-09 2004-07-09 检测蛋白-蛋白相互作用的方法 Expired - Lifetime CN1894581B (zh)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US48596803P 2003-07-09 2003-07-09
US60/485,968 2003-07-09
US51191803P 2003-10-15 2003-10-15
US60/511,918 2003-10-15
US56611304P 2004-04-27 2004-04-27
US60/566,113 2004-04-27
PCT/US2004/021887 WO2005007822A2 (en) 2003-07-09 2004-07-09 Method for assaying protein-protein interaction

Publications (2)

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CN1894581A CN1894581A (zh) 2007-01-10
CN1894581B true CN1894581B (zh) 2012-02-01

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US (3) US7049076B2 (f)
EP (2) EP1644734B1 (f)
JP (1) JP4734498B2 (f)
CN (1) CN1894581B (f)
AT (1) ATE534900T1 (f)
AU (1) AU2004257228B8 (f)
CA (1) CA2531698A1 (f)
IL (1) IL173023A0 (f)
WO (1) WO2005007822A2 (f)

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CN104991072B (zh) * 2015-06-16 2017-03-29 西北农林科技大学 一种昆虫体外蛋白质相互作用检测系统的制备方法及应用
KR102808969B1 (ko) 2016-01-11 2025-05-15 더 보드 어브 트러스티스 어브 더 리랜드 스탠포드 주니어 유니버시티 키메라 단백질 및 면역요법 방법
EA201891619A1 (ru) 2016-01-11 2019-02-28 Те Борд Оф Трастиз Оф Те Лилэнд Стэнфорд Джуниор Юниверсити Химерные белки и способы регулирования экспрессии генов
GB201609604D0 (en) * 2016-06-01 2016-07-13 Ucl Business Plc Cell
CN107976411B (zh) * 2016-10-25 2020-10-23 中国科学院微生物研究所 包含别构转录因子调控系统的生物传感器、试剂盒及其在小分子检测中的用途
WO2019062744A1 (zh) * 2017-09-27 2019-04-04 北京大学 融合多肽
CN108191979B (zh) * 2017-12-08 2021-01-01 上海晶诺生物科技有限公司 一种荧光互补检测人趋化因子生物学活性的方法
JP7367974B2 (ja) 2018-07-31 2023-10-24 国立大学法人 東京大学 膜タンパク質活性測定法
CN110853712B (zh) * 2018-08-01 2022-06-07 清华大学 鉴定多对生物分子间相互作用调控因子的方法
KR102317202B1 (ko) * 2018-10-19 2021-10-25 주식회사 프로티나 유방암 재발 예측 방법
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CN113423436B (zh) * 2018-12-10 2024-02-06 耶鲁大学 塑造宿主生理学的微生物群代谢物
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AU2004257228A1 (en) 2005-01-27
EP2336768A9 (en) 2011-08-10
CA2531698A1 (en) 2005-01-27
US20120077706A1 (en) 2012-03-29
ATE534900T1 (de) 2011-12-15
US7049076B2 (en) 2006-05-23
IL173023A0 (en) 2006-06-11
EP1644734A2 (en) 2006-04-12
AU2004257228B8 (en) 2009-01-08
US20050100934A1 (en) 2005-05-12
US8017398B2 (en) 2011-09-13
JP2007528713A (ja) 2007-10-18
AU2004257228B2 (en) 2008-12-18
EP1644734B1 (en) 2011-11-23
US20060147975A1 (en) 2006-07-06
JP4734498B2 (ja) 2011-07-27
CN1894581A (zh) 2007-01-10
WO2005007822A2 (en) 2005-01-27
WO2005007822A3 (en) 2005-03-31
EP1644734A4 (en) 2007-04-25
EP2336768A1 (en) 2011-06-22

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