CN1894401B - 从人胚胎干细胞衍生终末分化的多巴胺能神经元 - Google Patents
从人胚胎干细胞衍生终末分化的多巴胺能神经元 Download PDFInfo
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| WO2009148170A1 (ja) * | 2008-06-06 | 2009-12-10 | 独立行政法人理化学研究所 | 幹細胞の培養方法 |
| EP2356450B1 (en) * | 2008-10-24 | 2019-02-06 | B.R.A.H.M.S GmbH | Prognosis and risk assessment in stroke patients by determining the level of marker peptides |
| CN101824399B (zh) * | 2009-03-05 | 2012-05-30 | 中日友好医院 | 从人羊膜上皮细胞中诱导分化出多巴胺能神经元的方法及分离所得神经元的用途 |
| CN101824398B (zh) * | 2009-03-05 | 2012-08-08 | 中日友好医院 | 人羊膜上皮细胞与神经干细胞共培养诱导分化多巴胺能神经元的方法 |
| WO2011055855A1 (en) | 2009-11-05 | 2011-05-12 | Riken | A method for differentiation induction in cultured stem cells |
| WO2011108766A1 (en) * | 2010-03-03 | 2011-09-09 | Kyoto University | METHOD FOR DIAGNOSING A PROTEIN MISFOLDING DISEASE USING NERVE CELLS DERIVED FROM iPS CELLS |
| CN110684734A (zh) * | 2011-11-04 | 2020-01-14 | 纪念斯隆-凯特琳癌症中心 | 用于植入的中脑多巴胺(da)神经元 |
| KR102073111B1 (ko) * | 2012-10-16 | 2020-02-04 | 메르츠 파마 게엠베하 운트 코. 카가아 | 신경독소 폴리펩티드의 생물학적 활성의 결정을 위한 세포 테스트 시스템 |
| SG11201601720RA (en) * | 2013-09-05 | 2016-04-28 | Univ Kyoto | New method for inducing dopamine-producing neural precursor cells |
| ES2972541T3 (es) * | 2014-09-08 | 2024-06-13 | Riken | Método para producir tejido progenitor cerebeloso |
| CN105087475B (zh) * | 2015-09-17 | 2018-12-04 | 广州赛莱拉干细胞科技股份有限公司 | 一种细胞培养液及其应用以及诱导牙髓干细胞向神经样细胞分化的方法 |
| JP2018529389A (ja) * | 2015-10-08 | 2018-10-11 | ニューロナ セラピューティクス インコーポレイテッドNeurona Therapeutics Inc. | 神経前駆細胞集団およびそれらの使用 |
| US11898163B2 (en) | 2016-04-22 | 2024-02-13 | Kyoto University | Method for producing dopaminergic neuron progenitor cell |
| FR3058892B1 (fr) * | 2016-11-23 | 2021-04-09 | Univ Bordeaux | Unite de tissu neural et utilisation d'une telle unite pour l'implantation dans le systeme nerveux d'un mammifere |
| EP3551748B1 (en) * | 2016-12-12 | 2022-07-13 | Health and Biotech France (H&B France) | Perinatal tissue derived mesenchymal stem cells: method of preparation and uses thereof |
| WO2020054647A1 (ja) * | 2018-09-14 | 2020-03-19 | 学校法人慶應義塾 | アストロサイトの製造方法 |
| CN111304167B (zh) * | 2018-12-12 | 2024-03-26 | 上海泉眼生物科技有限公司 | 人源脂肪干细胞来源的神经元前体细胞及其制备方法和应用 |
| JP7209850B2 (ja) * | 2019-01-22 | 2023-01-20 | コリア ユニバーシティ リサーチ アンド ビジネス ファウンデーション | 直接細胞転換に基づく神経幹細胞の星状膠細胞への分化方法 |
| CN114606188A (zh) * | 2019-03-06 | 2022-06-10 | 安徽中盛溯源生物科技有限公司 | 一种中脑多巴胺能神经前体细胞的制备方法及其应用 |
| CN110257332A (zh) * | 2019-07-08 | 2019-09-20 | 广东省赛莱拉干细胞研究院 | 一种诱导间充质干细胞分化成为多巴胺能神经元的培养基以及方法 |
| CN110669143B (zh) * | 2019-10-10 | 2021-05-14 | 中国人民解放军军事科学院军事医学研究院 | 一种短肽及其应用 |
| CN114657119B (zh) * | 2022-03-16 | 2023-10-20 | 广东省农业科学院动物科学研究所 | 一种初生仔猪肠神经胶质细胞的分离和原代培养方法 |
| CN115591025B (zh) * | 2022-11-09 | 2024-01-02 | 深圳先进技术研究院 | 神经调控器件、制备方法及其应用 |
| JP2025539432A (ja) | 2022-12-02 | 2025-12-05 | ナウェーセル バイオテクノロジーズ カンパニー リミテッド | 中脳ドーパミン作動性前駆細胞を成熟させるための培養培地、コーティングマトリックスおよび方法 |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6277820B1 (en) * | 1998-04-09 | 2001-08-21 | Genentech, Inc. | Method of dopaminergic and serotonergic neuron formation from neuroprogenitor cells |
| WO2003000868A1 (en) * | 2001-06-21 | 2003-01-03 | Geron Corporation | Dopaminergic neurons and proliferation-competent precursor cells for treating parkinson's disease |
Family Cites Families (2)
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| AU2001263199B2 (en) * | 2000-05-17 | 2004-09-16 | Asterias Biotherapeutics, Inc. | Neural progenitor cell populations |
| US20030036195A1 (en) * | 2001-04-20 | 2003-02-20 | Lorenz Studer | Generation of differentiated tissue from nuclear transfer embryonic stem cells and methods of use |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| US6277820B1 (en) * | 1998-04-09 | 2001-08-21 | Genentech, Inc. | Method of dopaminergic and serotonergic neuron formation from neuroprogenitor cells |
| WO2003000868A1 (en) * | 2001-06-21 | 2003-01-03 | Geron Corporation | Dopaminergic neurons and proliferation-competent precursor cells for treating parkinson's disease |
Non-Patent Citations (2)
| Title |
|---|
| Alexandra Rolletschek et al..Differentiation of embryonic stem cell-derived dopaminergicneurons is enhanced by survival-promoting factors.Mechanisms of Development105.2001,10594-95. * |
| WO 03000868 A1,说明书第11页第18行至第12页22行. |
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