CN1894401B - 从人胚胎干细胞衍生终末分化的多巴胺能神经元 - Google Patents

从人胚胎干细胞衍生终末分化的多巴胺能神经元 Download PDF

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CN1894401B
CN1894401B CN2004800127488A CN200480012748A CN1894401B CN 1894401 B CN1894401 B CN 1894401B CN 2004800127488 A CN2004800127488 A CN 2004800127488A CN 200480012748 A CN200480012748 A CN 200480012748A CN 1894401 B CN1894401 B CN 1894401B
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nidogen
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stem cell
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CN1894401A (zh
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S·M·托泰伊
G·拉温德兰
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Reliance Life Sciences Pvt Ltd
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CN2004800127488A 2003-03-12 2004-03-11 从人胚胎干细胞衍生终末分化的多巴胺能神经元 Expired - Fee Related CN1894401B (zh)

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Application Number Priority Date Filing Date Title
IN269MU2003 2003-03-12
IN269/MUM/2003 2003-03-12
US52004803P 2003-11-14 2003-11-14
US60/520,048 2003-11-14
PCT/IB2004/001246 WO2004081172A2 (en) 2003-03-12 2004-03-11 Derivation of terminally differentiated dopaminergic neurons from human embryonic stem cells

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CN1894401A CN1894401A (zh) 2007-01-10
CN1894401B true CN1894401B (zh) 2012-05-23

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Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009148170A1 (ja) * 2008-06-06 2009-12-10 独立行政法人理化学研究所 幹細胞の培養方法
EP2356450B1 (en) * 2008-10-24 2019-02-06 B.R.A.H.M.S GmbH Prognosis and risk assessment in stroke patients by determining the level of marker peptides
CN101824399B (zh) * 2009-03-05 2012-05-30 中日友好医院 从人羊膜上皮细胞中诱导分化出多巴胺能神经元的方法及分离所得神经元的用途
CN101824398B (zh) * 2009-03-05 2012-08-08 中日友好医院 人羊膜上皮细胞与神经干细胞共培养诱导分化多巴胺能神经元的方法
WO2011055855A1 (en) 2009-11-05 2011-05-12 Riken A method for differentiation induction in cultured stem cells
WO2011108766A1 (en) * 2010-03-03 2011-09-09 Kyoto University METHOD FOR DIAGNOSING A PROTEIN MISFOLDING DISEASE USING NERVE CELLS DERIVED FROM iPS CELLS
CN110684734A (zh) * 2011-11-04 2020-01-14 纪念斯隆-凯特琳癌症中心 用于植入的中脑多巴胺(da)神经元
KR102073111B1 (ko) * 2012-10-16 2020-02-04 메르츠 파마 게엠베하 운트 코. 카가아 신경독소 폴리펩티드의 생물학적 활성의 결정을 위한 세포 테스트 시스템
SG11201601720RA (en) * 2013-09-05 2016-04-28 Univ Kyoto New method for inducing dopamine-producing neural precursor cells
ES2972541T3 (es) * 2014-09-08 2024-06-13 Riken Método para producir tejido progenitor cerebeloso
CN105087475B (zh) * 2015-09-17 2018-12-04 广州赛莱拉干细胞科技股份有限公司 一种细胞培养液及其应用以及诱导牙髓干细胞向神经样细胞分化的方法
JP2018529389A (ja) * 2015-10-08 2018-10-11 ニューロナ セラピューティクス インコーポレイテッドNeurona Therapeutics Inc. 神経前駆細胞集団およびそれらの使用
US11898163B2 (en) 2016-04-22 2024-02-13 Kyoto University Method for producing dopaminergic neuron progenitor cell
FR3058892B1 (fr) * 2016-11-23 2021-04-09 Univ Bordeaux Unite de tissu neural et utilisation d'une telle unite pour l'implantation dans le systeme nerveux d'un mammifere
EP3551748B1 (en) * 2016-12-12 2022-07-13 Health and Biotech France (H&B France) Perinatal tissue derived mesenchymal stem cells: method of preparation and uses thereof
WO2020054647A1 (ja) * 2018-09-14 2020-03-19 学校法人慶應義塾 アストロサイトの製造方法
CN111304167B (zh) * 2018-12-12 2024-03-26 上海泉眼生物科技有限公司 人源脂肪干细胞来源的神经元前体细胞及其制备方法和应用
JP7209850B2 (ja) * 2019-01-22 2023-01-20 コリア ユニバーシティ リサーチ アンド ビジネス ファウンデーション 直接細胞転換に基づく神経幹細胞の星状膠細胞への分化方法
CN114606188A (zh) * 2019-03-06 2022-06-10 安徽中盛溯源生物科技有限公司 一种中脑多巴胺能神经前体细胞的制备方法及其应用
CN110257332A (zh) * 2019-07-08 2019-09-20 广东省赛莱拉干细胞研究院 一种诱导间充质干细胞分化成为多巴胺能神经元的培养基以及方法
CN110669143B (zh) * 2019-10-10 2021-05-14 中国人民解放军军事科学院军事医学研究院 一种短肽及其应用
CN114657119B (zh) * 2022-03-16 2023-10-20 广东省农业科学院动物科学研究所 一种初生仔猪肠神经胶质细胞的分离和原代培养方法
CN115591025B (zh) * 2022-11-09 2024-01-02 深圳先进技术研究院 神经调控器件、制备方法及其应用
JP2025539432A (ja) 2022-12-02 2025-12-05 ナウェーセル バイオテクノロジーズ カンパニー リミテッド 中脳ドーパミン作動性前駆細胞を成熟させるための培養培地、コーティングマトリックスおよび方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6277820B1 (en) * 1998-04-09 2001-08-21 Genentech, Inc. Method of dopaminergic and serotonergic neuron formation from neuroprogenitor cells
WO2003000868A1 (en) * 2001-06-21 2003-01-03 Geron Corporation Dopaminergic neurons and proliferation-competent precursor cells for treating parkinson's disease

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2001263199B2 (en) * 2000-05-17 2004-09-16 Asterias Biotherapeutics, Inc. Neural progenitor cell populations
US20030036195A1 (en) * 2001-04-20 2003-02-20 Lorenz Studer Generation of differentiated tissue from nuclear transfer embryonic stem cells and methods of use

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6277820B1 (en) * 1998-04-09 2001-08-21 Genentech, Inc. Method of dopaminergic and serotonergic neuron formation from neuroprogenitor cells
WO2003000868A1 (en) * 2001-06-21 2003-01-03 Geron Corporation Dopaminergic neurons and proliferation-competent precursor cells for treating parkinson's disease

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Alexandra Rolletschek et al..Differentiation of embryonic stem cell-derived dopaminergicneurons is enhanced by survival-promoting factors.Mechanisms of Development105.2001,10594-95. *
WO 03000868 A1,说明书第11页第18行至第12页22行.

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