CN1839887A - Spider freeze-drying powder preparation method and its uses in medicine and health product - Google Patents
Spider freeze-drying powder preparation method and its uses in medicine and health product Download PDFInfo
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- CN1839887A CN1839887A CNA2005101207772A CN200510120777A CN1839887A CN 1839887 A CN1839887 A CN 1839887A CN A2005101207772 A CNA2005101207772 A CN A2005101207772A CN 200510120777 A CN200510120777 A CN 200510120777A CN 1839887 A CN1839887 A CN 1839887A
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- aranea
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Abstract
The invention provides a process for preparing freeze-dried spider powder and the use in preparing medicaments and health products, wherein the preparing process consists of preprocessing, freezing, drying and disintegrating. The powder can be used for preparing fatigue-resisting health wine, or medicine for treating arthritis.
Description
Technical field
The present invention relates to the processing method and the application thereof of animal product, relate in particular to a kind of preparation method of spider freeze-drying powder and the application in medicine and health product.
Background technology
Aranea just has the record of being used as medicine in Compendium of Material Medica, cry in " elegant ": inferior
Claim in " dialect ":
Yu is: Rainbow again
Abnormal smells from the patient is slightly cold, and is slightly poisonous.Be mainly used in: " skin ulcer is swollen, body odour, moth-eaten tooth for main mouthful snail, proctoptosis." " Mingyi Bielu " record: can treat adult, children's Collapse hernia, and big abdomen fourth a surname of children's, can not walk in 3 years." Newly Revised Canon of Materia Medica " meaning: " preventing or arresting vomiting is contrary for main snake venom pyrexial malaria, cholera." useful fire is got Aranea friction side cheek in " prescriptions worth thousand gold ", the air port snail is oblique in being used for the treatment of.Modern medicine study proves that there are treatment thrush, oral aphthae in children, nasal polyp, venom etc. in the clinical practice aspect.Report that some countries of Japan, the U.S. and West Europe steep in wine with the work Aranea, be used for the damp eliminating eliminating inflammation and expelling toxin.Aspect medical science, the range of application of Aranea is in continuous expansion, spider venom injection, and Aranea ball, pure spider powder, bromhidrosis spirit, rehabilitation pill for brains increase a little grade and are applied to the clinical kind more than 200 that reaches.
What in the Aranea disease is had therapeutic efficiency mainly is spider venom.The spider venom main component is polypeptide and protein, comprise polypeptide neurotoxins, cytotoxin, enzyme, kassinin kinin analog, agglutination activity peptide and antibacterial peptide etc., contain polyamines class and ATP, nucleotide, aminoacid, monoamines low molecular weight substance and inorganic salt etc. in addition.
The tradition tcm methods is that usefulness method such as dry, dry is made Aranea is dried or be ground into the Aranea powder again, also have water or ethanol etc. to extract, all there is heating process in these methods, can cause the loss of active component, also abundant inadequately to extraction of active ingredients, also there is the not high problem of efficient.
Chinese patent CN93104722.6 discloses a kind of Aranea live body inducing poison-taking method, and still this method is operated by hand, and efficient is not high, and exists spider bites to get malicious person's danger, and this in addition method can not guarantee to get complete active component in the Aranea.
Summary of the invention
An object of the present invention is to provide a kind of preparation method of spider freeze-drying powder.
The preparation method of spider freeze-drying powder of the present invention comprises the steps: pre-treatment, freezing, lyophilizing and pulverizing.
In one embodiment, the preparation method of spider freeze-drying powder comprises:
(1) pre-treatment
Water cleans fresh Aranea corpse, perhaps it is dismembered again;
(2) freezing
Liquid nitrogen or-20 ℃ and more under the low temperature Aranea corpse after the freezing cleaning or its limbs to freeze real till;
(3) lyophilizing
In the freeze dryer of pre-cooling, in-45 ℃ or more low temperature and 0.1 standard atmosphere depress dry Aranea or its limbs after freezing, control drying time is 8-15 hour;
(4) pulverize
Aranea corpse after the pulverizing lyophilizing or its limbs and sealing are preserved.
In another embodiment, the preparation method of spider freeze-drying powder comprises:
(1) pre-treatment
Water cleans fresh Aranea corpse, perhaps it is dismembered again;
(2) freezing
Liquid nitrogen or-20 ℃ and more under the low temperature Aranea corpse after the freezing cleaning or its limbs to freeze real till;
(3) pulverize
Aranea corpse after freezing or its limbs are pulverized;
(4) lyophilizing
In the freeze dryer of pre-cooling, in-45 ℃ or dry Aranea powder under low temperature and 0.1 standard atmosphere more, control drying time is 8-15 hour, collects also sealing and preserves.
Aranea used in the present invention can select for use but the Aranea that is not limited to just like Selenocosmia huwena (Selenocosmia Huwena), Jingzhao chilobrachy spider (Chilobrachys jingzhao), Chilean red rose (Chilobrachys jingzhao) and sherry Criny spider (Atrax Robustus) etc.Preferred Selenocosmia huwena and Jingzhao chilobrachy spider.
Spider freeze-drying powder of the present invention is as the application in the medicine of treatment of arthritis.
Spider freeze-drying powder of the present invention is as the application in the analgesic.
The application of spider freeze-drying powder of the present invention in preparation anti-fatigue health-caring wine.
The present invention prepares the method for spider freeze-drying powder, the production efficiency height, and active ingredient loss is little in the Aranea, and process is simple.
The specific embodiment
Below come the present invention is further described with specific embodiment, but embodiment does not constitute any limitation of the invention.
Embodiment 1
Get one of firm adult Selenocosmia huwena corpse, about 30 grams of quality through killing.With clear water the Aranea corpse is rinsed well, with its dismemberent.Immediately with the Aranea limbs with sealing the plastic bag splendid attire and placing liquid nitrogen container freezing 1 hour, the Aranea limbs are frozen reality.Aranea limbs after freezing are placed on lyophilization in the commonly used common freeze dryer of-45 ℃ of pre-coolings after 1 hour, and the lyophilizing built-in temperature is reduced to-50 ℃, pressure is 0.1 normal atmosphere in the evacuation control freeze dryer, and drying was closed freeze dryer in 12 hours.Grind Aranea limbs after the lyophilizing with mortar in dry environment, obtain Aranea powder 6.7 grams, finished product is preserved in sealing.
Embodiment 2
Get one of firm adult Jingzhao chilobrachy spider corpse, about 17 grams of quality through killing.With clear water the Aranea corpse is rinsed well.Immediately with Aranea with sealing the plastic bag splendid attire and putting in-70 ℃ the cryogenic refrigerator freezing 7 hours, the Aranea corpse is frozen reality.The Aranea corpse that grinds in dry environment after freezing with mortar obtains the Aranea powder.The Aranea powder is placed on cold doing in the commonly used common freeze dryer of-45 ℃ of pre-coolings after 1 hour, and the lyophilizing built-in temperature is reduced to-50 ℃, pressure is 0.1 normal atmosphere in the evacuation control freeze dryer, closes freeze dryer in dry 8 hours, collection obtains Aranea powder 3.3 grams, and finished product is preserved in sealing.
Embodiment 3
The application of spider freeze-drying powder in health product:
Component and consumption: every 1000ml 38 degree Chinese liquor, spider freeze-drying powder 1 gram that makes among the embodiment 1, Hippocampus 1 gram, Cornu Cervi Pantotrichum 1 gram, Radix Ginseng Rubra 2 grams, Fructus Lycii 20 grams, Herba Epimedii 12 grams, Fructus Schisandrae Chinensis 12 grams.
Technical process: the good raw material of weighing in proportion, pulverize, add 1/2nd Chinese liquor, vibration or stir and spend the night is filtered.In dregs, add remaining 1/2nd Chinese liquor then, repeat the operation of front, merging filtrate.Allocate mouthfeel with Mel.
The main health care of spider health care wine is a resisting fatigue, and through mice swimming with a load attached to the body test, administration group swimming time is 5 times of blank group, is 2 hours.Resisting fatigue effect highly significant.
Embodiment 4
The application of spider freeze-drying powder in the treatment of arthritis medicine:
Be configured to contain the medicated wine of spider freeze-drying powder 0.1% with the spider freeze-drying powder that makes among 38 degree Chinese liquor and the embodiment 1.
The effect experiment: through rat test, the arthritis that two kinds of proinflammatory agents of formaldehyde and Freund's complete adjuvant cause, the spontaneous recovery in 15 days of blank group, the administration group was cured in 5 days.
Test method: adopt formaldehyde to cause scorching method, with 2% formaldehyde 0.1ml, be expelled between the three or four joint of rat right rear leg earlier, cause the arthritis generation, after one day, arthroncus reaches maximum.Divide blank group and administration group to compare then, every group has eight rats, and the administration group is irritated 0.2 milliliter of this routine medicated wine of stomach one time every day, writes down the girth (cm) in joint and the time and the calculating mean value of arthritis disappearance respectively and obtains result such as following table:
| Project | Initial joint girth | Administration first day | Administration second day | Administration the 3rd day | Administration the 4th day | Administration the 5th day | Administration the 6th day | Administration the 7th day | Administration the 8th day | Administration the 9th day | Administration the tenth day |
| Normal rat | 3.6 | - | - | - | - | - | - | - | - | - | - |
| Blank group | 4.5 | 4.7 | 4.5 | 4.2 | 3.9 | 3.9 | 3.9 | 3.8 | 3.8 | 3.7 | 3.6 |
| The administration group | 4.5 | 3.6 | 3.6 | 3.6 | 3.6 | 3.6 | - | - | - | - | - |
As seen the medicated wine that makes has positive effect to treatment of arthritis.
Embodiment 5
The application of spider freeze-drying powder in the analgesia product:
Lyophilized powder is directly as medicine, and the Aranea Scorpio lyophilized powder with 35 degree Chinese liquor addings 1% makes medicated wine.
Effect experiment: adopt the mouse writhing test, give 0.2 milliliter of medicated wine that makes of eight mouse stomaches earlier, use 0.6% acetic acid 0.2ml then, allow mice cause pain.Eight mices of blank group directly cause bitterly with 0.6% acetic acid 0.2ml, observe then in 60 minutes turn round the body number of times and calculating mean value obtains result such as following table:
| Group number | Sample | Early start is turned round the body time | 60min on average turns round the body number of times |
| 1 | This routine medicated wine | 15min begins to turn round body | 8 |
| 2 | Blank | 3min begins to turn round body | 36 |
As seen the medicated wine analgesic effect that makes is obvious.
Claims (6)
1. the preparation method of a spider freeze-drying powder is characterized in that comprising the steps: pre-treatment, freezing, lyophilizing and pulverizing.
2. the preparation method of spider freeze-drying powder according to claim 1 is characterized in that preparation process comprises:
(1) pre-treatment
Water cleans fresh Aranea corpse, perhaps it is dismembered again;
(2) freezing
Liquid nitrogen or-20 ℃ and more under the low temperature Aranea corpse after the freezing cleaning or its limbs to freeze real till;
(3) lyophilizing
In the freeze dryer of pre-cooling, in-45 ℃ or more low temperature and 0.1 standard atmosphere depress dry Aranea or its limbs after freezing, control drying time is 8-15 hour;
(4) pulverize
Aranea corpse after the pulverizing lyophilizing or its limbs and sealing are preserved.
3. the preparation method of spider freeze-drying powder according to claim 1 is characterized in that preparation process comprises:
(1) pre-treatment
Water cleans fresh Aranea corpse, perhaps it is dismembered again;
(2) freezing
Liquid nitrogen or-20 ℃ and more under the low temperature Aranea corpse after the freezing cleaning or its limbs to freeze real till;
(3) pulverize
Aranea corpse after freezing or its limbs are pulverized;
(4) lyophilizing
In the freeze dryer of pre-cooling, in-45 ℃ or more low temperature and 0.1 standard atmosphere depress dry Aranea powder, control drying time is 8-15 hour, collects also sealing and preserves;
4. spider freeze-drying powder is as the application in the medicine of treatment of arthritis.
5. spider freeze-drying powder is as the application in the medicine of analgesic.
6. spider freeze-drying powder is as the application in the antifatigue health promoting wine.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2005101207772A CN1839887A (en) | 2005-12-22 | 2005-12-22 | Spider freeze-drying powder preparation method and its uses in medicine and health product |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2005101207772A CN1839887A (en) | 2005-12-22 | 2005-12-22 | Spider freeze-drying powder preparation method and its uses in medicine and health product |
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| CN1839887A true CN1839887A (en) | 2006-10-04 |
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| CNA2005101207772A Pending CN1839887A (en) | 2005-12-22 | 2005-12-22 | Spider freeze-drying powder preparation method and its uses in medicine and health product |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008077277A1 (en) * | 2006-12-25 | 2008-07-03 | Graduate School At Shenzhen, Tsinghua University | Process for preparation of spider lyophilizate and use in medicaments and functional foods |
| CN103638057A (en) * | 2013-12-01 | 2014-03-19 | 大理学院 | Preparation method and medical application of nephila spider extract anticancer part |
| CN104547490A (en) * | 2015-01-04 | 2015-04-29 | 海南蛛王药业有限公司 | Medicine for treating periodontal disease and preparation method of medicine |
| CN104758221A (en) * | 2015-01-08 | 2015-07-08 | 海南蛛王药业有限公司 | Spider freeze-dried powder mosquito repellent liquid and preparation method thereof |
| CN106333968A (en) * | 2016-08-29 | 2017-01-18 | 梁鹤耀 | Method for preparing freeze-dried spider powder |
| CN112973928A (en) * | 2021-04-28 | 2021-06-18 | 山东银丰生命科学研究院 | Method and apparatus for treating carcasses using microwave drying and air flow milling |
| CN113144020A (en) * | 2021-05-12 | 2021-07-23 | 海南蛛王生物科技有限公司 | Traditional Chinese medicine composition for treating arthralgia, preparation method thereof and traditional Chinese medicine preparation |
-
2005
- 2005-12-22 CN CNA2005101207772A patent/CN1839887A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008077277A1 (en) * | 2006-12-25 | 2008-07-03 | Graduate School At Shenzhen, Tsinghua University | Process for preparation of spider lyophilizate and use in medicaments and functional foods |
| CN103638057A (en) * | 2013-12-01 | 2014-03-19 | 大理学院 | Preparation method and medical application of nephila spider extract anticancer part |
| CN104547490A (en) * | 2015-01-04 | 2015-04-29 | 海南蛛王药业有限公司 | Medicine for treating periodontal disease and preparation method of medicine |
| CN104547490B (en) * | 2015-01-04 | 2018-04-13 | 海南蛛王药业有限公司 | A kind of medicine for treating periodontal disease and preparation method thereof |
| CN104758221A (en) * | 2015-01-08 | 2015-07-08 | 海南蛛王药业有限公司 | Spider freeze-dried powder mosquito repellent liquid and preparation method thereof |
| CN106333968A (en) * | 2016-08-29 | 2017-01-18 | 梁鹤耀 | Method for preparing freeze-dried spider powder |
| CN112973928A (en) * | 2021-04-28 | 2021-06-18 | 山东银丰生命科学研究院 | Method and apparatus for treating carcasses using microwave drying and air flow milling |
| CN113144020A (en) * | 2021-05-12 | 2021-07-23 | 海南蛛王生物科技有限公司 | Traditional Chinese medicine composition for treating arthralgia, preparation method thereof and traditional Chinese medicine preparation |
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