CN1235601C - Extractive of ocean star worm, its preparing method and application - Google Patents
Extractive of ocean star worm, its preparing method and application Download PDFInfo
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Abstract
The present invention relates to an extract of effective components of ocean sipunculids, and a preparation method and the application of the extract. The extract of effective components of ocean sipunculids adopts ocean sipunculids as raw materials. A salt solution is used for extracting, and an organic solvent is used for fractional deposition and ultrafiltration. After the raw materials are frozen and dried, the extract of active components is obtained. The main functional components of the present invention are active protein, taurine, arginine, trace elements, zinc, etc. The extract of ocean sipunculids can obviously improve the immune functions of bodies by the research of immunology, so a spleen index and a thymus index of the body can be obviously improved, and a lymphocyte transformation rate is obviously improved. The extract of ocean sipunculids has the obvious function of resisting fatigue by fatigue resisting tests of animals, and can obviously prolong the loading swimming time of mice. The present invention can accelerate the elimination of blood lactic acid of animals, and can reduce the serum urea nitrogen of animals during movement. The extract of ocean sipunculids can obviously delay aging, obviously reduce the content of serum MDA of big mice, impove the activity of serum SOD, and prolong the living time of fruit flies by the aging delaying research of animals. Therefore, the extract of ocean sipunculids has the functions of improving the immune functions of bodies, resisting fatigue and delaying aging.
Description
Technical field:
The present invention relates to a kind of marine organism extract and preparation method, be specifically related to a kind of ocean Sipunculoidea active component extract and preparation method and application.
Background technology:
The ocean Sipunculoidea has another name called HAICAN sand, HAICAN, sea sand worm, and it is not only the seafood treasure of delicious flavour, also is a kind of low sugar, low fat, high protein and the high-grade nutrient health care food that is rich in several mineral materials such as zinc, ferrum, calcium, phosphorus, iodine.
The ocean Sipunculoidea is except that work is edible, and is among the people also its Dietotherapy medicament as treatment hyperactivity of deficient fire and enriching yin and nourishing kidney.Generally be used for conjunctival congestion, flush.Summer, dog days were exposed to the sun because of burning sun, health heating, the patient of constipation, but the relief of symptoms of using.Because of overfatigue, limbs fatigue, many clothes can be in high spirits, energy recovers rapidly, so the south of Fujian Province people is referred to as " animal Radix Ginseng ".Coastal among the people commonly used replacing expensive Cordyceps, thereby be again " Cordyceps in the ocean " by laudatory title.In addition, in Fujian, coastal areas such as platforms, Guangdong, fine jade, fisherman's daily life of a family has dietary functions such as spleen invigorating nourishing with the ocean Sipunculoidea feeding young children that cooks congee.
The health care of ocean Sipunculoidea, how on the books in Ancient Times in China medicine monograph.The ocean Sipunculoidea is regarded as " extra large medicine " since ancient times, native soil, southern part of the country scenery is known in the famous herbal scholar Lee Xun Guang trip south of the Five Ridges of Tang end during five generations, is familiar with the medicinal animals and plants in the southern part of the country, show " Haiyao Bencao, Oversea Materia Medica " and put down in writing a large amount of external medicines, wherein just put down in writing " HAICAN sand ", it " gives birth between the stone of mountain, the South Sea its silkworm shape; big as thumb; that sand is very white, as beautiful powdery, joint is arranged whenever " cloud.HAICAN sand " salty in the mouth, big temperature, nontoxic.Main asthenia cold air, Chu's wind is unsuccessful.Clothes make us gloss for a long time, and tonify deficiency is thin, makes light of one's life by commiting suicide, and it is not old to prolong life ".Famous herbal scholar's Li Shizhen (1518-1593 A.D.), its equally also record the medicinal efficacy of " HAICAN " among the Compendium of Material Medica of showing.The traditional Chinese medical science thinks that ocean Sipunculoidea nature and flavor are sweet, salty, cold, and lung heat clearing tonify deficiency, nourishing YIN to lower pathogenic fire effect are arranged, and clinical practice confirms diseases such as osteopyrexia and fever, night sweat due to yin deficiency, cough and asthma due to lung deficiency, abundant expectoration uncomfortable in chest, and edible ocean Sipunculoidea is effective in cure.To illness such as pulmonary tuberculosis cough, neurasthenia, infantile spleen deficiencies, good therapeutic effect is also arranged as if stewing with ocean Sipunculoidea and Rhizoma Zingiberis pot.With Huaihe River Qi Yuan meat pot ocean Sipunculoidea soup, not only nutritious, delicious flavour, and have the effect of YIN nourishing skin care.Efficacious prescriptions among the people have: 1. the sea sand worm is fried in shallow oil: get sea sand worm 30g, decocting boils, 1 dose, be decocted in water for oral dose every day 2 times, have nourishing YIN to lower pathogenic fire, removing heat from the lung and dissipating phlegm effect, cure mainly pulmonary tuberculosis, the cough spit, hectic fever night sweat; 2. add flavor sea sand worm soup: get sea sand worm 10g, Radix Stemonae 15g, Radix Glycyrrhizae 5g; Decocting, 1 dose, be decocted in water for oral dose every day 2 times, have nourishing YIN and moistening the lung, eliminating phlegm and stopping cough effect, cure mainly the YIN-deficiency type pulmonary tuberculosis.
Modern medicine study finds that the hydrolyzed extract of ocean Sipunculoidea (P.Similis) has the effect of vasoconstrictive, quickening heart rate, enhancing heartbeat output and coronary flow, and losing blood property hypotension is had remarkable boosting, and the good sedative analgesic effect is also arranged.Manufactured goods-native Bulbus Allii of usefulness ocean Sipunculoideas such as Huang Zheyuan freezes the study of pharmacy result who carries out and shows, the aqueous extract of ocean Sipunculoidea has obvious anti-fatigue, anoxia enduring, resistant to elevated temperatures effect to mice; Find that they contain 6-7 kind essential amino acid, especially with Lys, Leu, Val content is higher; Especially zinc content is very high in the trace element, can reach 68 μ g/g.Yu Zhen very waits the people to test and finds that mud Bulbus Allii-Penaeus seu panulirus mixture has tangible galgctogogue action.
Summary of the invention:
Technical problem to be solved by this invention is to adopt modern biochemical technology, separates obtaining its active component from the Sipunculoidea of ocean, and a kind of nutrient and healthcare products or medicine with remarkable resisting fatigue and delaying senility function is provided.
Ocean disclosed by the invention Sipunculoidea active component extract is that to select the ocean Sipunculoidea for use be raw material, uses the saline solution extracting, organic solvent fractional precipitation, ultrafiltration, the active component extract that steps such as lyophilizing are obtained.Concrete is that employing ocean Sipunculoidea is a raw material, with the phosphate buffer extracting that contains 1～2mol/L hydrochlorate and 1～10mmol/L EDTA, high speed centrifugation, supernatant final concentration are 20%～70% dehydrated alcohol fractional precipitation, collect 20%～70% dehydrated alcohol deposited components, vacuum lyophilization, dry thing dissolving back is 10,000 and 100,000 ultrafilter membrane ultrafiltration respectively with molecular cut off, collects 1～100,000 component, and is concentrated, lyophilizing obtains.
This active constituent extract contains active ingredient protein, taurine, trace element and arginine, wherein Protein content 〉=55%.
Protein molecular weight in the active component extract of the present invention accounts for total protein content more than 18% at three kinds of activated protein content of 10,000,2.8 ten thousand, 3.1 ten thousand, accounts for the total extract active component content more than 5.67%.
Another technical problem to be solved by this invention is to disclose above-mentioned ocean Sipunculoidea active component preparation method of extract.
Ocean of the present invention Sipunculoidea active component extract obtains by following technical proposals:
Ocean Sipunculoidea raw material cleans up back homogenate in the buffer solution of the saliferous hydrochlorate of 2～5 times of volumes, room temperature is extracted 24h, 8000rpm high speed centrifugation 30min, supernatant final concentration are 20%～70% dehydrated alcohol fractional precipitation, collect 20%～70% dehydrated alcohol deposited components, vacuum lyophilization, dry thing dissolving back is 10,000 and 100,000 ultrafilter membrane ultrafiltration respectively with molecular cut off, collects 1～100,000 component, and is concentrated, lyophilizing promptly obtains ocean Sipunculoidea active component.
The buffer solution of saliferous hydrochlorate of the present invention can be selected 10～20mmol/L PB solution or other phosphate buffer for use, wherein contains 1～2mol/L hydrochlorate and 1～5mmol/LEDTA, and described hydrochlorate can be selected NaCl, KCl etc. for use.
Active peak of the present invention can be identified with the test determination of MTT cytoactive or with the SDS-PAGE electrophoresis.
Another technical problem to be solved by this invention is to disclose the application of above-mentioned ocean Sipunculoidea active component extract in preparation resisting fatigue and anti-aging health care product or medicine.
Ocean of the present invention Sipunculoidea active component can be used as the active ingredient of resisting fatigue and anti-aging health care food and related drugs, according to health care and treatment needs, makes various preparations with edible and pharmaceutic adjuvant.
It is object of study that Kunming mouse is selected in the antifatigue effect research of ocean of the present invention Sipunculoidea for use, set up the ocean Sipunculoidea sample sets of basic, normal, high three dosage, dosage is respectively 0.04,0.09,0.26g/kg, be equivalent to 5,10,30 times of human body recommended dose respectively, other sets up the distilled water matched group.Every treated animal number is 10, gastric infusion, and the filling gastric capacity is 0.4ml/20g.The result shows that this product does not have obvious influence to the weight of animals, can obviously prolong the mice swimming with a load attached to the body time, obviously accelerates motion back mice blood lactic acid and eliminates, and significantly reduces the serum urea nitrogen content; Illustrate that ocean Sipunculoidea extract has the obvious anti-fatigue effect.
It is object of study that the Study on antioxidation of the deferring senility of ocean of the present invention Sipunculoidea is selected the SD rat for use, set up the ocean Sipunculoidea sample sets of basic, normal, high three dosage, dosage is respectively 0.04,0.09,0.26g/kg, be equivalent to 5,10,30 times of human body recommended dose respectively, other sets up distilled water matched group and young animal control groups.Every treated animal number is 10, gastric infusion, and the filling gastric capacity is 1ml/100g, successive administration 30 days.Results suggest ocean Sipunculoidea extract can reduce SD rat blood serum MDA content, increases activity of SOD in serum, illustrates that ocean Sipunculoidea extract has antioxidation.
Resisting fatigue and delaying senility function to ocean of the present invention Sipunculoidea extract is further described below:
Ocean of the present invention Sipunculoidea active component rich in proteins, taurine, trace element, arginine isoreactivity material.
Taurine (Taurine) has another name called 2-aminoethane sulfinic acid, is the highest free amino acid of cell intensive amount.It has the Recent study proof statocyte osmotic pressure, keeps cell membrane stability, regulates cytoprotections such as cell calcium stable state and antagonism oxygen free radical injury.The main pharmacological activity of taurine has: A, analgesic, analgesia, antiinflammatory; B, calmness, calm the nerves; C, protection cell membrane, hepatic cholagogic; D, antiviral, anti-microbial pathogen; E, detoxify, separate chemical poisoning, pre-anti-poisoning etc.The major physiological regulating action of taurine has: A, promotion brain development, regulate nerve conduction, and improve brain function, suppress and the treatment alzheimer disease; B, raising body specificity and nonspecific immunity; Visual performance is kept in C, enhancing and improve vision; The absorption of D, promotion nutrient substance calcium, lipid, vitamin; E, improve child's anorexia and dyssomnia, promote upgrowth and development of children.
Arginine can be regulated the level of the body cell factor, promotes infants growth and development, promotes repair in trauma, minimizing stress the time proteinic excessive degradation.Arginine can increase the weight of thymus, stimulates the various CD cells of thymic lymphocytes and thymic cortex and medullary substance, thus the enhancing human body immunity function.
Zinc is that research is maximum at present, and health function is trace element the most clearly, has antibiont peroxidating, promotion growth, human body immunity improving function, enhances skeletal growth, prevents various health-care such as sexual hypofunction, auxiliary blood sugar lowering.
Protein is the important substance basis of body vital movement, provides the proteic primary raw material of body tissue, is the primary structure composition that constitutes body, is an intravital important carrier; Protein is as enzyme, antibody, hormone etc. simultaneously, and the expression of the regulation and control of the Growth and Differentiation of pair cell and hereditary information in vital movement plays an important role; Be the indispensable material bases of various metabolism.We studies show that ocean Sipunculoidea extract protein content more than 55%, and wherein molecular weight is about 10,000, and 2.8 ten thousand, 3.1 ten thousand three kinds of activated protein content account for 18% of total protein, and the content of three kinds of activated proteins is 5.67% in the Sipunculoidea extract of ocean.We find that these three kinds of albumen have resisting fatigue, the senile-resistant efficacy of highly significant
Carry out relevant efficacy test with ocean of the present invention Sipunculoidea extract:
1, ocean Sipunculoidea extract can significantly improve body's immunity
Discover that ocean Sipunculoidea extract gastric infusion has remarkable potentiation to mouse spleen and thymic weight index, high dose group P value is all less than 0.01 (as table 1); Can significantly promote mouse lymphocyte to transform, dosage significantly improves lymhocyte transformation rate (P＜0.05) during for 0.2g/kgd, and highly significant improved lymhocyte transformation rate (P＜0.01) (as table 2) when dosage was 1.0g/kgd.Illustrate that ocean Sipunculoidea extract has significant facilitation to body's immunity.
Table 1 ocean Sipunculoidea extract gastric infusion is to the influence of mouse spleen and thymus index
| Group | Number of animals (only) | Dosage (g/kgd) | Administering mode (my god) | Spleen index (mg/g) | Thymus index (mg/g) |
| | 9 | 0 | Po×20 | 4.04±0.33 | 2.22±0.47 |
| The ocean Sipunculoidea | 11 | 0.2 | Po×20 | 4.24±0.57 | 2.27±0.85 |
| The ocean Sipunculoidea | 10 | 1.0 | Po×20 | 4.66±0.74 ** | 2.33±0.35 ** |
Table 2 ocean Sipunculoidea extract gastric infusion is to the influence of mouse lymphocyte conversion ratio
| Group | Number of animals (only) | Dosage (g/kgd) | Administering mode (my god) | Drench rate of rotation | The P value |
| | 9 | 0 | Po×20 | 0.047±0.028 | |
| The ocean Sipunculoidea | 11 | 0.2 | Po×20 | 0.092±0.062 | ＜0.05 |
| The ocean Sipunculoidea | 10 | 1.0 | Po×20 | 0.142±0.111 | ＜0.01 |
2, ocean Sipunculoidea extract has significant antifatigue effect
(1) ocean Sipunculoidea extract can the significant prolongation mice swimming with a load attached to the body time
Adopt mice swimming with a load attached to the body test, result such as table 3.Behind the Kunming mouse oral administration 30 days, swimming with a load attached to the body time significant prolongation, the dosage group swimming with a load attached to the body time has been compared significance prolongation (P＜0.05) in the administration with matched group.The high dose group swimming with a load attached to the body time has been compared highly significant and has prolonged (P＜0.01) with matched group.
Table 3 sample is to the influence of mice swimming with a load attached to the body time (X ± SD)
| Dosage (g/kg.d) | Number of animals (only) | The swimming with a load attached to the body time (second) |
| Matched | 10 | 456±125 |
| 0.04 | 10 | 527±180 |
| 0.09 | 10 | 577±152 * |
| 0.26 | 10 | 687±136 ** |
(2) ocean Sipunculoidea extract can significantly be accelerated the elimination of animal blood lactic acid
Behind the Kunming mouse oral administration 30 days, after quiet 30 minutes, measure mice blood lactate level respectively in 30 minutes, back 0 minute of (2% body weight) swimming of bearing a heavy burden, swimming after heat wind dry doubling after the last administration.The result as shown in Figure 1, before the motion and back 0 minute each administration treated animal blood lactate level of motion and the equal no significant difference of control animals (P＞0.05), move back 30 minutes blood lactate levels of administration treated animal descend high dose group decline highly significant (P＜0.05) gradually along with the increase of dosage.
(3) ocean Sipunculoidea extract can significantly reduce motion mice serum blood urea nitrogen
Behind the Kunming mouse oral administration 30 days, after the last administration 30 minutes, mice is put into 30 ℃ of water temperatures swimming 90 minutes, hot-air seasoning after the peace and quiet, is got serum and is surveyed blood urea nitrogen.The results are shown in Figure 2, along with the increase of dosage, the mice serum blood urea nitrogen descends gradually, and high dose group has significant difference.
The ocean Sipunculoidea extract that The above results prompting per os gives the mice various dose is after 30 days, and the mice swimming with a load attached to the body time obviously prolongs, and motion back mice blood lactic acid is eliminated obviously and accelerated, and the serum urea nitrogen content descends significantly; Illustrate that ocean Sipunculoidea extract has the obvious anti-fatigue effect.
3, ocean Sipunculoidea extract has significant delaying senility function
1) ocean Sipunculoidea extract has significant antioxidant role
The SD male white rat at 20 monthly ages is an object of study, is divided into basic, normal, high three dosage groups, and other establishes aged animal control groups and young animal control groups (giving distilled water), every group of 10 animals.The continuous oral administration in the 31st day, was plucked eyeball and is got blood after 30 days, centrifuging and taking serum.Superoxide dismutase (SOD) vigor in lipid peroxide catabolite malonaldehyde (MDA) content and the blood in the mensuration blood.
Ocean Sipunculoidea extract is as shown in table 4 to the influence of SD rat body weight.Each dosage group of sample is compared difference that there are no significant with aged control.Ocean Sipunculoidea extract compares with aged matched group influence such as Fig. 3 of SD rat blood serum lipid peroxide catabolite malonaldehyde (MDA) content, and each administration group is along with the MDA content that increases of dosage descends gradually, and high dose group descends obviously.Ocean Sipunculoidea extract compares with aged matched group influence such as Fig. 4 of SD rat blood serum superoxide dismutase (SOD) vigor, and each administration group is along with the SOD activity that increases of dosage rises gradually, and high dose group rises significantly.
Table 4 ocean Sipunculoidea extract is to the influence of SD rat body weight
| Group | Number of animals | Initial body weight | Mid-term body weight | Final body weight |
| | 10 | 485±14 | 512±12 | 541±16 |
| Low dosage (0.04g/kg.d) | 10 | 480±16 | 511±18 | 538±15 |
| Middle dosage (0.09g/kg.d) | 10 | 482±16 | 510±18 | 541±18 |
| High dose (0.26g/kg.d) | 10 | 480±18 | 513±16 | 546±20 |
| | 10 | 78±4 * | 140±11 * | 187±11 * |
The above results prompting ocean Sipunculoidea extract can reduce SD rat blood serum MDA content, increases activity of SOD in serum, illustrates that ocean Sipunculoidea extract has antioxidation.
2) ocean Sipunculoidea extract can obviously prolong the drosophila survival time
New emergence fruit bat adult was an object of study in eight hours, 400 every group, and male and female half and half.If 0.006%, 0.017%, 0.052% and 0.156% 4 a dosage group and a matched group (conventional corn powder), every group of sample number is 200.Cultivate under 25 ± 1 ℃ of conditions of temperature, changed freshly prepared culture medium once in per four days, per two days observed and recorded drosophila survival numbers and death toll are till whole fruit bat death.Calculate three indexs such as half death time, average life and average maximum life span.The result is as shown in table 5.The ocean Sipunculoidea of high dose can the female male drosophila of significant prolongation average life and the average maximum life span of male drosophila, illustrate that ocean Sipunculoidea extract has the effect of life lengthening.
Table 5 ocean Sipunculoidea extract is to the influence of drosophila survival
| Sample concentration (%) | Average weight (μ g) | The half death time (my god) | Average life (my god) | Average maximum life span (my god) | ||||
| Male | Female | Male | Female | Male | Female | | Female | |
| 0 | 611 | 632 | 55 | 69 | 55±9 | 68±9 | 72±4 | 80±2 |
| 0.006 | 609 | 633 | 55 | 71 | 55±12 | 68±10 | 73±3 | 80±2 |
| 0.017 | 613 | 628 | 55 | 71 | 56±11 | 68±10 | 74±4 | 80±2 |
| 0.052 | 612 | 629 | 57 | 72 | 56±11 | 70±8 | 74±2 | 81±2 |
| 0.156 | 609 | 635 | 58 | 72 | 59±11 * | 71±7 * | 76±4 * | 81±2 |
Above-mentioned result of the test ocean Sipunculoidea extract has antioxidation and effect of prolonging life, illustrates that ocean Sipunculoidea extract has delaying senility function.
4, conclusion
Ocean of the present invention Sipunculoidea extract adopts the pollution-free natural ocean Sipunculoidea in the North Sea, Guangxi to extract refining forming.Be rich in multiple nutritional components; as contain 8 seed amino acids of needed by human and multiplely have a reactive protein that important biomolecule is learned function; contain and enrich natural taurine and needed by human body mineral and trace element, be widely used in child, old man and patient, have plurality of health care functions.The testing result explanation ocean Sipunculoidea of we and Shanghai Research Center for Preventive Medicine has obvious anti-fatigue and function of delaying senility, ocean Sipunculoidea extract can strengthen the endurance of animal under stress state, significantly improve period of decline mice survival rate, enhancing body SOD activity has tangible anti-aging effects.Has the two-ways regulation function.Have defying age and life lengthening function.
Land Cordyceps enjoys ancient Chinese medicine doctor to pay attention to because of its unique curative effect, tonic effect are described as the panacea of weakness, and is famous at home and abroad.Common saying cloud " one of peaceful Cordyceps, the not full car of gold and jade " foot shows that it is famous and precious.But, because the destruction of Qinghai-Tibet weather conditions makes the Qinghai Province, the main place of production of Cordyceps warm winter and prolonged drought occur, caused falling sharply of cordyceps yield, and made these product face the danger of extinction in recent years.
The ocean Sipunculoidea is extremely similar with Cordyceps on functional component, as unique natural substitute for of just endangered land Cordyceps, and its aboundresources, but artificial cultivation again, with low cost, exploitation ocean Sipunculoidea will become the gold industry of 21 century.
Description of drawings:
Fig. 1, ocean Sipunculoidea extract are to the influence of motion mice blood lactic acid
Fig. 2, to the influence of serum urea nitrogen behind the mouse movement
Fig. 3, ocean Sipunculoidea extract are to the influence of SD rat MDA content
Fig. 4, ocean Sipunculoidea extract are to the active influence of SD rat blood serum SOD
The active component content of Fig. 5, embodiment 1 ocean Sipunculoidea extract
The active component content of Fig. 6, embodiment 2 ocean Sipunculoidea extracts
The specific embodiment
Ocean Sipunculoidea raw material cleans up back homogenate in the 10mmol/lPB buffer solution that contains 2mol/lNaCl of 2 times of volumes, room temperature is extracted 24h, 8000rpm high speed centrifugation 30min, the supernatant final concentration is 20%～70% dehydrated alcohol fractional precipitation, collect 20%～70% dehydrated alcohol deposited components, vacuum lyophilization, dry thing dissolving back is 10,000 and 100,000 ultrafilter membrane ultrafiltration respectively with molecular cut off, the component of collection 1～100,000, concentrate, lyophilizing promptly obtains ocean Sipunculoidea active component.Each active ingredient content of active component is seen Fig. 5.
Embodiment 2
Ocean Sipunculoidea raw material cleans up back homogenate in the 20mmol/lPB buffer solution that contains 1mol/lKCl of 5 times of volumes, room temperature is extracted 24h, 10000rpm high speed centrifugation 20min, the supernatant final concentration is 20%～70% dehydrated alcohol fractional precipitation, collect 20%～70% dehydrated alcohol deposited components, vacuum lyophilization, dry thing dissolving back is 10,000 and 100,000 ultrafilter membrane ultrafiltration respectively with molecular cut off, the component of collection 1～100,000, concentrate, lyophilizing promptly obtains ocean Sipunculoidea active component.Each active ingredient content of active component is seen Fig. 6.
Claims (7)
1, a kind of ocean Sipunculoidea extract with resisting fatigue and delaying senility function, it is characterized in that this extract is that employing ocean Sipunculoidea is a raw material, with the phosphate buffer extracting that contains 1～2mol/L hydrochlorate and 1～10mmol/L EDTA, high speed centrifugation, the supernatant final concentration is 20%～70% dehydrated alcohol fractional precipitation, collect 20%～70% dehydrated alcohol deposited components, vacuum lyophilization, dry thing dissolving back is 10,000 and 100,000 ultrafilter membrane ultrafiltration respectively with molecular cut off, the component of collection 1～100,000, concentrate, lyophilizing obtains.
2, a kind of ocean as claimed in claim 1 Sipunculoidea extract is characterized in that this extract contains protein, taurine, trace element and arginine.
3, a kind of ocean as claimed in claim 1 Sipunculoidea extract is characterized in that wherein Protein content is 55%.
4, a kind of ocean as claimed in claim 1 Sipunculoidea preparation method of extract is characterized in that this extract obtains by following method:
Ocean Sipunculoidea raw material cleans up back homogenate in the buffer solution of the saliferous hydrochlorate of 2～5 times of volumes, room temperature is extracted 24h, 8000rpm high speed centrifugation 30min, supernatant final concentration are 20%～70% dehydrated alcohol fractional precipitation, collect 20%～70% dehydrated alcohol deposited components, vacuum lyophilization, dry thing dissolving back is 10,000 and 100,000 ultrafilter membrane ultrafiltration respectively with molecular cut off, collects 1～100,000 component, and is concentrated, lyophilizing, promptly.
5, a kind of ocean as claimed in claim 4 Sipunculoidea preparation method of extract, the buffer solution that it is characterized in that wherein said saliferous hydrochlorate is selected from 10～20mmol/L phosphate buffer, wherein contain 1～2mol/L hydrochlorate and 1～10mmol/L EDTA, described hydrochlorate is selected from NaCl or KCl.
6, the application of a kind of ocean as claimed in claim 1 Sipunculoidea extract in preparation resisting fatigue and anti-aging health care product or medicine.
7, the application of a kind of ocean as claimed in claim 7 Sipunculoidea extract is characterized in that the active ingredient of this extract as resisting fatigue and anti-aging health care food and related drugs, makes various preparations with edible and pharmaceutic adjuvant.
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