CN103169081B - Sandworm polypeptide enteric capsule - Google Patents
Sandworm polypeptide enteric capsule Download PDFInfo
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- CN103169081B CN103169081B CN201310092621.2A CN201310092621A CN103169081B CN 103169081 B CN103169081 B CN 103169081B CN 201310092621 A CN201310092621 A CN 201310092621A CN 103169081 B CN103169081 B CN 103169081B
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Abstract
The invention discloses a sandworm polypeptide enteric capsule. A sandworm polypeptide capsule is used as an antioxidant material which is applied to eliminating human body free radicals and is absorbed in a weak basic environment in the intestinal tract, so that the absorption rate and the bioavailability are improved. The sandworm polypeptide is extracted by steps of sandworm washing, enzyme hydrolysis, microfiltration, dialysis and the like. Then, the sandworm polypeptide powder is used together with mannitol, magnesium stearate and sodium deoxycholate to prepare the enteric capsule. The sandworm polypeptide enteric capsule provided by the invention has the antioxidant function, can eliminate human body free radicals, and is high in bioavailability and extremely easy to absorb.
Description
Technical field
The present invention relates to a kind of sandworm polypeptide enteric coated capsule, belong to biotechnology and food processing field.
Background technology
Sandworm claims again Sipunculus nudus
sipunculus nudus, be subordinate to sipunculan door, Genus Sipunculus, be without joint, vermiform marine products coelomate, water warm.Live in subtidal line area, all there is distribution in tropical and subtropical zone marine site.Sandworm is mainly distributed in southeastern coast in China, is one of the economic animal that dwells the large-scale end of Chinese mangrove area, and its meat is tender and crisp, delicious flavour, and rich in proteins, multiple amino acids and trace element, be a kind of dietotherapy excellent herbal cuisine of holding concurrently.In the multiple book on Chinese herbal medicine of China, recorded its edible and pharmaceutical use, it replaces Chinese medicine Cordyceps sinensis for some area, the disease such as cure mainly that bone hectic fever, night sweat due to yin deficiency, uncomfortable in chest, pulmonary tuberculosis cough, phlegm are many, nycturia and tooth swell and ache.Modern medicine research shows that siphon-worm contains various active material, can regulate body various physiological-function.
At present domestic sandworm is mainly studied to its edible effect and reproductive biology aspect, the research of sandworm albumen and polypeptide product thereof is but rarely had to report.Polypeptide not only has advantages of albumen, also there is good solubility and low viscosity simultaneously, absorption more easy to digest, and in sandworm, protein content is higher, must account for the more than 70% of total amino acid content by aminoacids content, therefore, can be using sandworm polypeptide as nutritive health-care food and functional food widen the Application Areas of sandworm.
As everyone knows, in people's orthobiosis, the generation of free radical is that inevitably people's interior free yl is more, and unhealthful factor is just more, and aging speed is just faster.Free radical only has with antioxidant and could remove, in body, be supplemented with enough antioxidant, human body just can be healthy, and generally acknowledged antioxidant is vitamin-E, vitamins C, carotene at present, secondly also has Vitamin P complex, natural astaxanthin, superoxide dismutase and tea-polyphenol etc.Through retrieval, find no the report of the application using sandworm polypeptide as antioxidant, the anti-oxidant free radical for removing in human body is so important, and the scope of widening antioxidant has just had very important meaning.Polypeptide is difficult for being completely absorbed under sour environment, and under weakly alkaline environment, is completely absorbed.Therefore, making to be absorbed under the weakly alkaline environment of sandworm polypeptide in enteron aisle, for improving the bioavailability of sandworm polypeptide in human body, is necessary.The technology that finds no sandworm polypeptide enteric coated capsule through retrieval discloses.
Summary of the invention
The object of this invention is to provide a kind of sandworm polypeptide enteric coated capsule, using the present invention as antioxidant, be applied to remove human free radical, and make to be absorbed under its weakly alkaline environment in enteron aisle, improve its specific absorption and bioavailability.
Foregoing invention object is achieved through the following technical solutions, and a kind of sandworm polypeptide enteric coated capsule is mainly prepared by following method:
(1) sandworm is cleaned: get fresh sandworm water and clean up, smash that to obtain sandworm slurry standby to pieces;
(2) enzymic hydrolysis: by sandworm slurry and the distilled water ratio of 1:2-8 by volume, add distilled water to stir, with the NaOH adjust pH of 1mol/L to 6-8, by the amount of every 0.2-2g/100mL, adding activity is that the papoid of 100,000 U/g and the flavor protease prozyme of 40,000 U/g are hydrolyzed, wherein papoid mixes in the ratio of 1:0.5-1.5 with flavor protease, in 30-60 ℃ of hydrolysis 4h, boils the 10min enzyme that goes out, the centrifugal 10min of 3000-5000r/min, obtains enzymolysis solution standby;
(3) micro-filtration and dialysis: enzymolysis solution carries out micro-filtration by aperture 30nm microfiltration membrane, removes suspended solid and bacterium; Dialysis membrane by 2000Da is dialysed, and removes total free aminoacids and low price salt ion, obtains molecular weight in the polypeptide liquid of 3000-20000Da, and the polypeptide powder that polypeptide liquid is obtained through lyophilize is standby;
(4) by following prescription, prepare:
Polypeptide powder 30%-60% N.F,USP MANNITOL 30%-60% Magnesium Stearate 0.5%-2.5%
Sodium desoxycholate 5%-10%;
(5) above-mentioned raw materials is mixed, technique is encased in enteric capsule shell routinely, obtains.
Take the present invention of above-mentioned measure to have the following advantages:
(1) there is anti-oxidant function.With a kind of prozyme, sandworm albumen is cut, by controlled enzymatic hydrolysis condition, prepare molecular weight at the polypeptide of 3000-20000Da, by DPPH free radical scavenging activity, test, concrete test method is in 1.5mLDPPH free-atom aqueous solution, to add 1.5mL liquid to be measured, concussion shakes up, place after 30min, at 517nm, go out to survey light absorption value A
1, blank for the ethanol of 1.5mL95% adds 1.5mL distilled water, contrast and add that 1.5mL distilled water is at the light absorption value A of mensuration wavelength for 1.5mL DPPH free radical
contrast, polypeptide solution is A at the light absorption value of measuring wavelength
2.Polypeptide represents with inhibiting rate R the removing ability of DPPH free radical: R%=[1-(A1-A2)/A
contrast] * 100, measure the polypeptide making the clearance rate of hydroxy radical qiao is reached to 60-90%, confirm to have obvious anti-oxidant activity.
(2) bioavailability is high.Oral polypeptide, protein medicaments are often degraded at upper digestive tract; bioavailability is low; by the parcel of enteric capsule shell, can avoid enzyme in hydrochloric acid in gastric juice and the upper digestive tract destruction to polypeptide; because added Sodium desoxycholate; it can protect polypeptide not to be hydrolyzed destruction; and expand intestinal mucosa pore radius, polypeptide is more easily absorbed.
Embodiment
Embodiment 1
Concrete preparation process:
(1) sandworm is cleaned: get fresh sandworm water and clean up, smash that to obtain sandworm slurry standby to pieces;
(2) enzymic hydrolysis: get 10000mL sandworm slurry and add 20000-60000mL distilled water to stir, NaOH adjust pH to 7 with 1mol/L, by the amount of every 1g/100mL, adding activity is that the papoid of 100,000 U/g and the flavor protease prozyme of 40,000 U/g are hydrolyzed, wherein the ratio of papoid and flavor protease is 1:0.5-1.5, in 30-60 ℃ of hydrolysis 4h, boil the 10min enzyme that goes out, the centrifugal 10min of 3000-5000r/min, obtains enzymolysis solution standby;
(3) micro-filtration and dialysis: enzymolysis solution carries out micro-filtration by aperture 30nm microfiltration membrane, removes suspended solid and bacterium; Dialysis membrane by 2000Da is dialysed, and removes total free aminoacids and low price salt ion, obtains molecular weight in the polypeptide liquid of 3000-20000Da, and the polypeptide powder that polypeptide liquid is obtained through lyophilize is standby;
(4) by following prescription, prepare:
0.5 gram of 30 grams of Magnesium Stearate of 30 grams of N.F,USP MANNITOL of polypeptide powder
5 grams of Sodium desoxycholates;
(5) above-mentioned raw materials is mixed, technique is encased in enteric capsule shell routinely, obtains.
Embodiment 2
Concrete preparation process:
(1) sandworm is cleaned: identical with embodiment 1;
(2) enzymic hydrolysis: get 10000mL sandworm slurry and add 20000-60000mL distilled water to stir, NaOH adjust pH to 7.5 with 1mol/L, by the amount of every 1.5g/100mL, adding activity is that the papoid of 100,000 U/g and the flavor protease prozyme of 40,000 U/g are hydrolyzed, wherein the ratio of papoid and flavor protease is 1:0.5-1.5, in 30-60 ℃ of hydrolysis 4h, boil the 10min enzyme that goes out, the centrifugal 10min of 3000-5000r/min, obtains enzymolysis solution standby;
(3) micro-filtration and dialysis: identical with embodiment 1;
(4) by following prescription, prepare:
2.5 grams of 60 grams of Magnesium Stearates of 60 grams of N.F,USP MANNITOL of polypeptide powder
10 grams of Sodium desoxycholates;
(5) above-mentioned raw materials is mixed, technique is encased in enteric capsule shell routinely, obtains.
Embodiment 3
Concrete preparation process is except (4), and all the other are identical with embodiment 1;
(4) by following prescription, prepare:
2 grams of 50 grams of Magnesium Stearates of 50 grams of N.F,USP MANNITOL of polypeptide powder
8 grams of Sodium desoxycholates.
Claims (1)
1. a sandworm polypeptide enteric coated capsule, is characterized in that described capsule mainly prepared by following method:
(1) sandworm is cleaned: get fresh sandworm water and clean up, smash that to obtain sandworm slurry standby to pieces;
(2) enzymic hydrolysis: by sandworm slurry and the distilled water ratio of 1:2-8 by volume, add distilled water to stir, with the NaOH adjust pH of 1mol/L to 6-8, by the amount of every 0.2-2g/100mL, adding activity is that the papoid of 100,000 U/g and the flavor protease prozyme of 40,000 U/g are hydrolyzed, wherein papoid mixes in the ratio of 1:0.5-1.5 with flavor protease, in 30-60 ℃ of hydrolysis 4h, boils the 10min enzyme that goes out, the centrifugal 10min of 3000-5000r/min, obtains enzymolysis solution standby;
(3) micro-filtration and dialysis: enzymolysis solution carries out micro-filtration by aperture 30nm microfiltration membrane, removes suspended solid and bacterium; Dialysis membrane by 2000Da is dialysed, and removes total free aminoacids and low price salt ion, obtains molecular weight in the polypeptide liquid of 3000-20000Da, and the polypeptide powder that polypeptide liquid is obtained through lyophilize is standby;
(4) by following prescription, prepare:
Polypeptide powder 30%-60% N.F,USP MANNITOL 30%-60% Magnesium Stearate 0.5%-2.5%
Sodium desoxycholate 5%-10%;
(5) above-mentioned raw materials is mixed, technique is encased in enteric capsule shell routinely, obtains.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310092621.2A CN103169081B (en) | 2013-03-22 | 2013-03-22 | Sandworm polypeptide enteric capsule |
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| CN201310092621.2A CN103169081B (en) | 2013-03-22 | 2013-03-22 | Sandworm polypeptide enteric capsule |
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| CN103169081A CN103169081A (en) | 2013-06-26 |
| CN103169081B true CN103169081B (en) | 2014-12-10 |
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Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104509853B (en) * | 2013-10-08 | 2016-09-14 | 宁波枫叶杰科生物技术有限公司 | A kind of instant Sipunculus nudus sheet |
| CN104146233B (en) * | 2014-04-30 | 2015-09-09 | 浙江省海洋水产养殖研究所 | Natural flavouring utilizing siphon-worm to prepare and preparation method thereof |
| CN104473185A (en) * | 2014-12-27 | 2015-04-01 | 广西壮族自治区海洋研究所 | Effervescent tablet containing vitamin C and sipunculusnudus enzymatic protein |
| CN105341618A (en) * | 2015-10-01 | 2016-02-24 | 常州市奥普泰科光电有限公司 | Preparation method of antioxidant for vegetable protein foods |
| CN106666707B (en) * | 2016-11-23 | 2020-04-28 | 广西壮族自治区海洋研究所 | Preparation method and application of Sipunculus nudus antioxidant extract |
| CN106636275A (en) * | 2016-12-12 | 2017-05-10 | 钦州学院 | Method for extracting proteins in sipunculus nudus |
| CN108030025A (en) * | 2017-12-08 | 2018-05-15 | 周起行 | The production method that a kind of antibacterial pickles radish |
| CN107811245A (en) * | 2017-12-08 | 2018-03-20 | 周起行 | A kind of preparation method of low-salt pickled radish |
| CN110638999A (en) * | 2019-08-30 | 2020-01-03 | 广东海洋大学 | Application of Sipunculus nudus collagen peptide in wound repair |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5576026A (en) * | 1994-09-30 | 1996-11-19 | Charter; Edward A. | Lumbricus product and method of making same |
| CN1430975A (en) * | 2003-01-23 | 2003-07-23 | 上海澳博海洋生物技术开发有限公司 | Extractive of ocean star worm, its preparing method and application |
| CN1628701A (en) * | 2004-08-27 | 2005-06-22 | 宁波大学 | Method for enzyme hydrolyzing ocean annulata species for pharmaceutical purpose and the product thereof |
| CN1748714A (en) * | 2004-09-14 | 2006-03-22 | 李妍妍 | Method for preparing biological active substance of siphon-worm and its products |
| CN101210261A (en) * | 2006-12-29 | 2008-07-02 | 宁波大学 | Method for preparing sipunculid polypeptide and application thereof |
-
2013
- 2013-03-22 CN CN201310092621.2A patent/CN103169081B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5576026A (en) * | 1994-09-30 | 1996-11-19 | Charter; Edward A. | Lumbricus product and method of making same |
| CN1430975A (en) * | 2003-01-23 | 2003-07-23 | 上海澳博海洋生物技术开发有限公司 | Extractive of ocean star worm, its preparing method and application |
| CN1628701A (en) * | 2004-08-27 | 2005-06-22 | 宁波大学 | Method for enzyme hydrolyzing ocean annulata species for pharmaceutical purpose and the product thereof |
| CN1748714A (en) * | 2004-09-14 | 2006-03-22 | 李妍妍 | Method for preparing biological active substance of siphon-worm and its products |
| CN101210261A (en) * | 2006-12-29 | 2008-07-02 | 宁波大学 | Method for preparing sipunculid polypeptide and application thereof |
Non-Patent Citations (2)
| Title |
|---|
| 朱银玲，等.方格星虫多肽的酶解法制备工艺优化与抗氧化作用研究.《化学通报》.2012,第75卷(第7期),第642-647页. * |
| 朱银玲，等.酶解法制备方格星虫多肽及其抗氧化作用研究.《化学研究与应用》.2012,第24卷(第9期),第1397-1401页. * |
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Application publication date: 20130626 Assignee: Beihai Bada Biotechnology Co. Ltd. Assignor: Xue Mingxiong Contract record no.: 2013450000050 Denomination of invention: Sandworm polypeptide enteric capsule License type: Exclusive License Record date: 20130528 |
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Address after: 536000 the Guangxi Zhuang Autonomous Region Beihai Seaview Avenue (Zhu Linduan) marine scientific research innovation park, one road B1-1 building Patentee after: Xue Mingxiong Address before: 536000 the Guangxi Zhuang Autonomous Region Beihai Haicheng District Sichuan South Road hi tech Zone entrepreneurial service center on the third floor Patentee before: Xue Mingxiong |
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