CN1628701A - Method for enzyme hydrolyzing ocean annulata species for pharmaceutical purpose and the product thereof - Google Patents
Method for enzyme hydrolyzing ocean annulata species for pharmaceutical purpose and the product thereof Download PDFInfo
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- CN1628701A CN1628701A CNA2004100540631A CN200410054063A CN1628701A CN 1628701 A CN1628701 A CN 1628701A CN A2004100540631 A CNA2004100540631 A CN A2004100540631A CN 200410054063 A CN200410054063 A CN 200410054063A CN 1628701 A CN1628701 A CN 1628701A
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Abstract
The invention relates to a method for enzyme hydrolyzing ocean annulata species for pharmaceutical purpose and the product thereof, wherein the method comprises, (1) washing the medicinal sea annulata, disintegrating and preparing water solution of different concentration, (2) charging enzyme into water solution for enzymolysis. The complex enzyme hydrolysate obtained can be used for further prepare oral liquid, capsule, electuary, and tablet.
Description
Technical field
The present invention relates to a kind of marine product manufactured goods and processing method thereof, specifically a kind of is raw material method of carrying out enzyme hydrolysis and products thereof with medicinal ocean annelid.
Background technology
Clam worm and Sipunculoidea all belong to medicinal ocean annelid.Clam worm claims Arenicola cristata stimpson., extra large Scolopendra, extra large centipede, Kattapillar, JINCHONG and blood worm etc. again.Body is vermiform, is made up of a lot of body segments, and in the perch silt, battalion lives in caves or the life of swimming, and belongs to the Omnivore.Adaptive capacity to temperature and salinity is stronger, and it is very wide to distribute in China coastal beach area, and resource is very abundant.But its nourishing YIN void, antiasthmatic-antitussive, hemopoietic, dampness removing and row are just.
Sipunculoidea is commonly called as HAIQU, sandworm, Hai Ding, native Radix Crotalariae szemoensis etc., is a kind of euryhaline organism, and not only meat is tender and crisp for it, delicious flavour, and has higher medicinal, food therapy value, can regulate the multiple function of body.Put down in writing its edible and medical value in the multiple pharmacopeia of China, Sipunculoidea is cold in nature, sweet in the mouth, one-tenth, have nourishing YIN to lower pathogenic fire, lung heat clearing tonify deficiency, invigorate blood circulation and keep fit and function such as the kidney invigorating skin care, can treat that osteopyrexia and fever, night sweat due to yin deficiency, the deficiency of the lung are breathed with cough, diseases such as abundant expectoration uncomfortable in chest and women's milk in puerperal rareness, to treatment pulmonary tuberculosis cough, neurasthenia, infantile spleen deficiency and deficiency of the kidney and nocturia is frequent etc. that disease all produces effect.Add that Sipunculoidea is a kind of nutritious; trace element such as rich in proteins, several amino acids and calcium, phosphorus, ferrum and can regulate the multiple composition of taurine of brain function; add that Sipunculoidea is a kind of high protein seafood that contains several amino acids; so the good reputation of " seabeach sausage " is arranged, favored by domestic and international consumer.
At present very single to the eating method of these two kinds of animals, mainly be directly edible.But because their nutritional labeling and structural similarity, their nutrient substance mostly is macromolecule greatly, be not easy directly to be absorbed by human body and utilize, particularly Functional Polypeptides and polysaccharide isoreactivity material do not dissociate out fully, make human body can not effectively transform and utilize these two kinds of animals nutrient substance, both increased production cost of products, reduced the effect of product again, utilization of nutrients is not high, but also contains certain toxin, easily human body is produced certain harm.
Chinese invention patent application CN1430975 A disclosed ocean Sipunculoidea effective component extracts and method thereof are that to select the ocean Sipunculoidea for use be raw material, use the saline solution extracting, the organic solvent fractional precipitation, and ultrafiltration obtains the active component extract after the lyophilization.The following shortcoming that this extracting method exists: 1, extracting method complexity, cost height.2, adopt organic solvent, have the latency of harmful to human.3, lose the existing nutrient substance of clam worm and Sipunculoidea easily.
Summary of the invention
Technical problem to be solved by this invention is to overcome the defective on the prior art and provide the medicinal ocean of a kind of enzyme hydrolysis annelid method, improve powerful active component, the content of oligomeric Functional Polypeptides, free amino acid and the polysaccharide of particularly high F value, accelerate these nutrient substance in the intravital conversion of people, promote human body to the absorption of these functional components, reach efficient, the low-cost medicinal annelid enzyme hydrolyzate of preparation and without organic solvent, avoid the purpose of secondary pollution.
Another object of the present invention is to utilize the annelid enzymic hydrolysates that contains rich functions peptide, free amino acid in above-mentioned medicinal ocean, preparation has oral liquid, capsule, tablet and the electuary of the hydrolysate of certain special physiological function, to improve medicinal, the nutritional activities composition of product, reach instant edible, and fully improve the annelid using value in medicinal ocean.
The present invention solves the problems of the technologies described above the scheme that is adopted:
The annelid method in the medicinal ocean of enzyme hydrolysis may further comprise the steps:
(1), medicinal ocean link class animal is cleaned, smashes and produce solution in different concentration;
(2), the adding enzyme carries out enzymolysis in its aqueous solution, makes enzyme hydrolyzate.
Main feature of the present invention is enzyme hydrolysis, can use papain, trypsin, 1389 neutral protease and compound protease, particularly adopts the compound protease hydrolysis effect better.Compound protease can be commercial enzyme, can buy from the market.Also can oneself produce.Compound protease contains cellulase, amylase and multiple protein enzyme.
Medicinal ocean link class animal is clam worm or Sipunculoidea, and the preparation of its aqueous solution can utilize fresh individuality, also can utilize the individuality of other states.
The content height of its Functional Polypeptides of enzyme hydrolyzate, polysaccharide and the free amino acid that obtains by said method.
A kind of enzyme hydrolyzate by method for preparing, this product contains rich functions peptide, polysaccharide and free amino acid, has human body immunity improving power, effect such as degeneration-resistant.
The use of above-mentioned enzyme hydrolyzate also can be made into oral liquid, capsule, tablet and electuary etc. for convenience.Because it is similar that this enzyme hydrolyzate liquid similar with other is made the method for various preparations, therefore as long as in said hydrolyzed liquid, add respective substance, just can be made into oral liquid, capsule, tablet and electuary, the preparation method of these preparations is known, does not do detailed argumentation.
Compared with prior art, the invention has the advantages that and select the annelid protein in the medicinal ocean of protease hydrolysis for use, without organic solvent, therefore easy and simple to handle, production cost is low, non-secondary pollution.The oligomeric Functional Polypeptides, polysaccharide and the free amino acid isoreactivity composition that contain natural active substance and more high F value in the enzyme hydrolyzate that makes, have better resisting fatigue, anoxia enduring, high temperature resistant, regulate multiple biological regulatory functions such as immunity, lactogenic.The special compound protease that uses, its method is easier, hydrolysis time is shorter, active substance transforms manyly.
The combinative enzyme hydrolysis liquid that utilizes said method to make can further be processed into oral liquid, capsule, electuary, tablet etc., and is edible more convenient like this, takes better effects if.
The specific embodiment
Below in conjunction with embodiment the present invention is described in further detail.
Embodiment 1:
Medicinal ocean link class animal is adopted clam worm or Sipunculoidea, and other medicinal ocean link class animals are also identical therewith.
The method of enzyme hydrolysis clam worm or Sipunculoidea is:
(1), fresh clam worm or Sipunculoidea water clean up, after the clam worm that cleans up or Sipunculoidea were pulverized with meat grinder, the reuse fiberizer further was milled to certain density aqueous solution.
(2), get the clam worm that makes in the step 1 or Sipunculoidea aqueous solution and add enzyme and carry out enzymolysis, obtain enzymolysis solution.
The addition of different clam worms or Sipunculoidea concentration and enzyme, temperature are selected the serviceability temperature of enzyme commonly used for use, and enzymolysis time is 4h, obtains different results, the condition of enzymolysis and the results are shown in Table 1 to table 16.
As can be seen from the table, adopt enzyme and compound protease commonly used, all can obtain ideal enzymolysis result, but the better effects if of compound protease.
Identical compound protease addition and different clam worms or Sipunculoidea concentration see Table 17 to table 20 with the result of variations of enzymolysis time.
As can be seen from the table, the compound protease enzymatic hydrolysis condition of clam worm and Sipunculoidea is preferably:
The concentration of clam worm or Sipunculoidea is 10-60% (weight), and concentration is the percentage ratio that clam worm and Sipunculoidea account for whole aqueous solution;
The addition of enzyme is for being 2 * 10
3IU/kg-1.4 * 10
6IU/kg (substrate);
Temperature is selected the serviceability temperature of composite zymoprotein enzyme for use, is generally 35-70 ℃, heats up gradually;
Enzymolysis time 2-6h.
Embodiment 2:
Add the lactose tabletting by clam worm that obtains among the embodiment 1 or Sipunculoidea enzymolysis solution and make tablet, the said goods has the function of resisting fatigue, human body immunity improving power; Clam worm or Sipunculoidea enzymolysis solution filtrate 1000: Mel 25: the Fructus Jujubae water cooking liquid filters 10: sugared 70 fills become oral liquid.
Embodiment 3
Enzymolysis solution directly or add behind the lactose spray drying to make powder encapsulated, the concrete ratio of lactose can be decided by the polysaccharide and the amino acid whose content of required product.
Embodiment 4
Enzymolysis solution is concentrated, and as inserts tabletting sugar coating, tablet is made in polishing with starch.
Embodiment 5
Electuary is made in adding lactose or beta cyclodextrin pelletize in enzymolysis solution.
Enzymatic hydrolysis condition and experimental result:
Amino acid content (mg/ml) behind the table 1 clam worm neutral proteinase hydrolysis
| Substrates enzymes concentration | 1.3×10 4IU/kg???1.1×10 5IU/kg??2.0×10 5IU/kg????3.0×10 5IU/kg |
| 10％ 20％ 40％ 40％ | 4.683????????????4.467????????????5.158??????????????5.935 4.046????????????4.231????????????4.033??????????????4.860 3.305????????????4.059????????????4.378??????????????4.551 3.01?????????????3.28?????????????4.09???????????????4.12 |
The content (mg/ml) of polysaccharide behind the table 2 clam worm neutral proteinase hydrolysis
| Substrates enzymes concentration | 1.3×10 4IU/kg????1.1×10 5IU/kg???2.0×10 5IU/kg????3.0×10 5IU/kg |
| 10％ 20％ 40％ 60％ | 160.43????????????233.77????????????321.65????????????404.90 158.88????????????182.67????????????253.27????????????264.22 136.32????????????171.21????????????199.49????????????244.95 123.36????????????136.02????????????141.32????????????133.69 |
Amino acid content (mg/ml) after the table 3 clam worm trypsin hydrolyzing
| Substrates enzymes concentration | 1.3×10 5IU/kg?????3.8×10 5IU/kg??6.3×10 5IU/kg??8.8×10 5IU/kg |
| 10％ 20％ 40％ 60％ | 5.417??????????????6.346????????????6.022???????????6.864 4.009??????????????4.855????????????5.810???????????4.922 4.620??????????????4.439????????????5.241???????????5.139 4.321??????????????4.481????????????4.487???????????4.571 |
The content (mg/ml) of polysaccharide after the table 4 clam worm trypsin hydrolyzing
| Substrates enzymes concentration | 1.3×10 5IU/kg????3.8×10 5IU/kg????6.3×10 5IU/kg????8.8×10 5I/kgU |
| 10％ 20％ 40％ 60％ | 105.59?????????????114.18????????????95.02?????????????106.91 90.54??????????????97.34?????????????75.06?????????????93.56 88.22??????????????96.42?????????????93.77?????????????75.80 77.89??????????????80.23?????????????81.46?????????????81.98 |
Amino acid content (mg/ml) after the table 5 clam worm papain hydrolysis
| Substrates enzymes concentration | 9.5×10 3IU/kg??7.6×10 4IU/kg??????1.5×10 5IU/kg |
| 10％ 20％ 40％ 60％ | 3.970????????????4.229???????????????4.359 3.750????????????4.157???????????????4.021 3.523????????????3.756???????????????3.765 3.215????????????3.349???????????????3.423 |
The content (mg/ml) of polysaccharide after the table 6 clam worm papain hydrolysis
| Substrates enzymes concentration | 9.5×10 3IU/kg???????7.6×10 4IU/kg????1.5×10 5IU/kg |
| 10％ 20％ 40％ 60％ | 120.79????????????????148.54????????????136.64 106.40????????????????125.65????????????119.61 115.71????????????????111.22????????????114.12 103.29????????????????113.54????????????113.89 |
Amino acid content (mg/ml) after the enzyme hydrolysis of table 7 clam worm compound protein
| Substrates enzymes concentration | 2×10 4IU/kg?????6×10 4IU/kg????1×10 4IU/kg???1.4×10 5IU/kg |
| 10％ 20％ 40％ 60％ | 10.966???????????13.529???????????19.023?????????21.171 9.325????????????6.999????????????23.863?????????24.062 7.712????????????7.597????????????15.930?????????16..576 6.327????????????9.190????????????10.219?????????11.309 |
Polyoses content (mg/ml) after the enzyme hydrolysis of table 8 clam worm compound protein
| Substrates enzymes concentration | 2×10 4IU/kg?????6×10 4IU/kg???1×10 4IU/kg????1.4×10 5IU/kg |
| 10％ 20％ 40％ 60％ | 76.20????????????120.32??????????180.39??????????199.93 99.31????????????76.18???????????220.52??????????193.36 107.15???????????108.43??????????147.61??????????178.53 107.13???????????85.65???????????96.77???????????121.61 |
Amino acid content (mg/m1) behind the table 9 Sipunculoidea neutral proteinase hydrolysis
| Substrates enzymes concentration | 1.3×10 4IU/kg??1.1×10 5IU/kg???2.0×10 5IU/kg????3.0×10 5IU/kg |
| 10％ 20％ 40％ 60％ | 3.841???????????4.121?????????????4.553?????????????4.985 3.899???????????4.173?????????????4.202?????????????4.274 3.778???????????3.767?????????????4.123?????????????3.961 3.808???????????3.713?????????????3.825?????????????4.145 |
The content (mg/ml) of polysaccharide behind the table 10 Sipunculoidea neutral proteinase hydrolysis
| Substrates enzymes concentration | 1.3×10 4IU/kg???1.1×10 5IU/kg??2.0×10 5IU/kg???3.0×10 5IU/kg |
| 10％ 20％ 40％ 60％ | 84.45????????????158.45???????????217.91???????????241.04 66.43????????????125.58???????????134.70???????????191.09 72.95????????????117.88???????????136.71???????????165.45 96.15????????????110.95???????????124.17???????????144.78 |
Amino acid content (mg/ml) after the table 11 Sipunculoidea trypsin hydrolyzing
| Substrates enzymes concentration | 1.3×10 5IU/kg??3.8×10 5IU/kg???6.3×10 5IU/kg???8.8×10 5IU/kg |
| 10％ 20％ 40％ 60％ | 4.078???????????4.208?????????????4.812????????????5.633 3.611???????????3.943?????????????4.017????????????4.317 3.508???????????4.177?????????????4.274????????????4.371 3.186???????????4.033?????????????3.938????????????4.439 |
Polyoses content (mg/ml) after the table 12 Sipunculoidea trypsin hydrolyzing
| Substrates enzymes concentration | 1.3×10 5IU/kg???3.8×10 5IU/kg???6.3×10 5IU/kg????8.8×10 5IU/kg |
| 10％ 20％ 40％ 60％ | 65.29????????????84.45?????????????89.07?????????????81.80 73.04????????????83.17?????????????76.56?????????????75.68 73.28????????????83.19?????????????75.59?????????????62.71 79.24????????????85.32?????????????72.10?????????????81.88 |
Amino acid content (mg/ml) after the table 13 Sipunculoidea papain hydrolysis
| Substrates enzymes concentration | 9.5×10 3IU/kg???7.6×10 4IU/kg??1.5×10 5IU/kg |
| 10％ 20％ 40％ 60％ | 2.826????????????3.646????????????3.949 2.589????????????3.151????????????3.367 2.309????????????2.914????????????2.968 2.193????????????2.953????????????2.849 |
The content (mg/ml) of polysaccharide after the table 14 Sipunculoidea papain hydrolysis
| Substrates enzymes concentration | 9.5×10 3IU/kg????7.6×10 4IU/kg??1.5×10 5IU/kg |
| 10％ 20％ 40％ 60％ | 120.79????????????148.54???????????136.64 106.4?????????????125.65???????????119.61 115.71????????????111.22???????????114.12 |
Amino acid content (mg/ml) after the enzyme hydrolysis of table 15 Sipunculoidea compound protein
| Substrates enzymes concentration | 2×10 4IU/kg?????6×10 4IU/kg???1×10 4IU/kg???1.4×10 5IU/kg... |
| 10％ 20％ 40％ 60％ | 11.571???????????13.558??????????19.567?????????22.309 9.395????????????12.374??????????14.678?????????16.404 5.063????????????11.542??????????15.117?????????14.579 11.947???????????13.530??????????12.357?????????14.012 |
The content (mg/ml) of polysaccharide after the enzyme hydrolysis of table 16 Sipunculoidea compound protein
| Substrates enzymes concentration | 2×10 4IU/kg?????6×10 4IU/kg???1×10 4IU/kg???1.4×10 5IU/kg... |
| 10％ 20％ 40％ 60％ | 138.65???????????90.19???????????124.31?????????150.39 74.11????????????104.03??????????169.65?????????151.19 95.34????????????82.90???????????100.17?????????100.21 50.63????????????60.38???????????76.69??????????81.41 |
The relation of amino acid content (mg/ml) and enzymolysis time in the table 17 clam worm compound protein enzyme hydrolyzate: the enzyme addition is: 5 * 10
4IU/kg, temperature adopts gradually and heats up
| Temperature/time concentration of substrate | 10％?????20％?????40％?????60％ |
| 35-70℃/2h 35-70℃/4h 35-70℃/6h | 19.23????18.26????18.59????17.36 29.23????33.45????28.46????25.79 20.45????22.12????19.56????14.45 |
The relation of polyoses content (mg/ml) and enzymolysis time in the table 18 clam worm compound protein enzyme hydrolyzate: the enzyme addition is: 5 * 10
4IU/kg, temperature adopts gradually and heats up
| Temperature/time concentration of substrate | ????10％??????20％??????40％??????60％ |
| 35-70℃/2h 35-70℃/4h 35-70℃/6h | ????99.31?????107.23????100.52????112.36 ????120.32????179.56????146.12????136.59 ????101.23????100.26????101.48????111.56 |
The relation of amino acid content (mg/ml) and enzymolysis time in the table 19 Sipunculoidea compound protein enzyme hydrolyzate: the enzyme addition is: 5 * 10
4IU/kg, temperature adopts gradually and heats up
| Temperature/time concentration of substrate | 10％??????20％??????40％??????60％ |
| 35℃-70℃/2h 35℃-70℃/4h 35℃-70℃/6h | 11.108????9.528?????6.926?????5.783 19.359????18.306????15.066????12.846 13.023????12.311????12.064????12.098 |
The relation of polyoses content (mg/ml) and enzymolysis time in the table 20 Sipunculoidea compound protein enzyme hydrolyzate: the enzyme addition is: 5 * 10
4IU/kg, temperature adopts gradually and heats up
| Temperature/time concentration of substrate | ?10％??????20％???????40％??????60％ |
| 35-70℃/2h 35-70℃/4h 35-70℃/6h | ?110.19????99.45??????96.23?????96.05 ?156.45????163.23?????144.56????139.02 ?121.03????132.25?????133.56????135.23 |
Claims (8)
1, the annelid method in the medicinal ocean of enzyme hydrolysis is characterized in that: may further comprise the steps:
(1), medicinal ocean link class animal is cleaned, smashes and produce solution in different concentration;
(2), the adding enzyme carries out enzymolysis in aqueous solution, makes enzyme hydrolyzate.
2, the annelid method in the medicinal ocean of enzyme hydrolysis as claimed in claim 1 is characterized in that: described enzyme is a compound protease.
3, the annelid method in the medicinal ocean of enzyme hydrolysis as claimed in claim 1 or 2, it is characterized in that: described medicinal ocean link class animal liquid enzymatic hydrolysis condition is: the content of medicinal ocean link class animal is 10-60% (weight), and the enzyme addition is 2 * 10
3IU/kg-1.4 * 10
6IU/kg; Hydrolysis temperature is the serviceability temperature of compound protease, and adopts intensification gradually; Enzymolysis time is 2-6h.
4, the enzymolysis solution that obtains by the annelid method of claim 1 or the medicinal ocean of 2 or 3 enzyme hydrolysiss.
5, medicinal ocean annelids enzyme hydrolyzate oral liquid is characterized in that: the medicinal ocean link class animal enzymolysis solution that is obtained by claim 4 adds Mel, Fructus Jujubae water cooking liquid, sugared fill.
6, medicinal ocean annelids enzyme hydrolyzate capsule is characterized in that: the medicinal ocean link class animal enzymolysis solution that is obtained by claim 4 adds lactose and forms.
7, medicinal ocean annelids enzyme hydrolyzate tablet is characterized in that: the medicinal ocean link class animal enzymolysis solution that is obtained by claim 4 adds starch and forms.
8, medicinal ocean annelids enzyme hydrolyzate electuary is characterized in that: the medicinal ocean link class animal enzymolysis solution that is obtained by claim 4 adds lactose or the beta cyclodextrin pelletize is made.
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| CNA2004100540631A CN1628701A (en) | 2004-08-27 | 2004-08-27 | Method for enzyme hydrolyzing ocean annulata species for pharmaceutical purpose and the product thereof |
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|---|---|---|---|
| CNA2004100540631A CN1628701A (en) | 2004-08-27 | 2004-08-27 | Method for enzyme hydrolyzing ocean annulata species for pharmaceutical purpose and the product thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103169081A (en) * | 2013-03-22 | 2013-06-26 | 薛命雄 | Sandworm polypeptide enteric capsule |
| CN106722934A (en) * | 2016-11-25 | 2017-05-31 | 济南睿呋康生物科技有限公司 | A kind of anti-ageing health food for containing peptide extract containing earthworm, clam worm |
| CN112352891A (en) * | 2020-10-27 | 2021-02-12 | 安徽济生元药业有限公司 | Sea sipunculus and sea cucumber polypeptide drink and preparation method thereof |
-
2004
- 2004-08-27 CN CNA2004100540631A patent/CN1628701A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103169081A (en) * | 2013-03-22 | 2013-06-26 | 薛命雄 | Sandworm polypeptide enteric capsule |
| CN103169081B (en) * | 2013-03-22 | 2014-12-10 | 薛命雄 | Sandworm polypeptide enteric capsule |
| CN106722934A (en) * | 2016-11-25 | 2017-05-31 | 济南睿呋康生物科技有限公司 | A kind of anti-ageing health food for containing peptide extract containing earthworm, clam worm |
| CN106722934B (en) * | 2016-11-25 | 2020-10-30 | 济南睿呋康生物科技有限公司 | Anti-aging health food containing earthworm and clam worm peptide-containing extract |
| CN112352891A (en) * | 2020-10-27 | 2021-02-12 | 安徽济生元药业有限公司 | Sea sipunculus and sea cucumber polypeptide drink and preparation method thereof |
| CN112352891B (en) * | 2020-10-27 | 2023-09-12 | 安徽济生元药业有限公司 | Sipunculus nudus and sea cucumber polypeptide drink and preparation method thereof |
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