WO2008077277A1

WO2008077277A1 - Process for preparation of spider lyophilizate and use in medicaments and functional foods

Info

Publication number: WO2008077277A1
Application number: PCT/CN2006/003585
Authority: WO
Inventors: Xuxu Zhang
Original assignee: Graduate School At Shenzhen, Tsinghua University; Well Treasure Technology Limited
Priority date: 2006-12-25
Filing date: 2006-12-25
Publication date: 2008-07-03

Abstract

The process for preparation of spider lyophilizate and use in manufacture of medicaments and functional foods thereof. Said process comprises pretreatment, freezing, lyophilization and pulverization. The present spider lyophilizate can be used in anti-fatigue functional foods and useful as medicaments for treating arthritis and analgesics.

Description

Preparation method of spider lyophilized powder and application in medicine and health care product

The invention relates to a method for treating animal products and an application thereof, in particular to a method for preparing a spider lyophilized powder and the application thereof in medicines and health care products.

Background technique

Spiders have long been recorded in the "Compendium of Materia Medica", and in the "Erya" is called: 賫. In "Dialects", it is called: 蛎蝓, also called: 蠛妩. The smell is slightly cold and there is a small poison. Mainly used for: "Main sinus, rectal prolapse, sore swollen, odorous, dental caries." "Medical doctors" recorded: can cure adults, children with ulcers, and children with abdominal abdomen, can not walk for three years. "Newly revised Materia Medica" says - "The main snake venom is warm, malaria, cholera, and vomiting." In "Qian Jin Fang", you can use a fire to take a spider to rub the cheeks, which is used to treat the sinus of the stroke. Modern medical research has proven that clinical applications include treatment of thrush, pediatric mouth sores, nasal polyps, and snake bites. It has been reported that some countries in Japan, the United States and Western Europe use live spiders to soak alcohol, detoxify and detoxify. In terms of medicine, the application range of spiders is expanding, and more than 200 kinds of spider venom injections, spider pills, pure spider powder, scented scent, brain rebuilding pills, and Zengwei No.1 have been used in clinical practice. Spider venom is the main therapeutic effect of the spider on the disease. The main components of spider venom are peptides and proteins, including polypeptide neurotoxins, cytotoxins, enzymes, kinin analogues, agglutination peptides and antimicrobial peptides, as well as polyamines and ATPs, nucleotides, amino acids, monoamines. Low molecular weight substances, inorganic salts, and the like.

The traditional Chinese medicine method is to dry or dry it into a spider's stem or pulverize it into a spider powder, or to extract it with water or ethanol. These methods all have a heating process, which will cause loss of the active ingredient, and the active ingredient The extraction is not enough, and there is still inefficiency. Question.

Chinese patent CN93104722.6 discloses a spider-inducing method for inducing poisoning, but this method is manually operated, inefficient, and there is a risk of spider bites being poisoned. In addition, this method cannot guarantee the integrity of the spider. Active ingredient.

Summary of the invention

It is an object of the present invention to provide a method of preparing a spider lyophilized powder.

The preparation method of the spider lyophilized powder of the present invention comprises the following steps: pretreatment, freezing, lyophilization and pulverization.

In one embodiment, the method for preparing a spider lyophilized powder comprises:

(1) pre-treatment

Wash the fresh spider corpse with water or dismember it;

(2) freezing

Frozen or washed spider corpses or limbs at room temperature or below -20 ° C and below until they are frozen;

(3) Freeze-dried

In a pre-cooled lyophilizer, the frozen spider or limb is dried at -45 Torr or lower and 0.1 standard atmosphere, and the drying time is controlled to be 8- 15 hours;

(4) crushing

The lyophilized spider corpse or its limbs are comminuted and sealed for storage.

In another embodiment, the method for preparing a spider lyophilized powder comprises:

(1) pre-treatment

Wash the fresh spider corpse with water or dismember it; Frozen or washed spider corpses or their limbs in liquid nitrogen or at -20 ° C and below until they are frozen;

(3) crushing

Crushing the frozen spider corpse or its limbs;

(4) Freeze-dried

In a pre-cooled lyophilizer, the spider powder is dried at -45 Torr or lower and 0.1 standard atmosphere, and the drying time is controlled for 8-15 hours, collected and sealed for storage.

The spider used in the present invention can be selected but not limited to spiders such as: tiger-shaped bird-trapping spider

Chilean red rose (CM.6 _rac / and Atrax Robustus, etc.) Tiger-like bird spider and godly hairy spider.

The spider lyophilized powder of the present invention is used as a medicament for treating arthritis.

The spider lyophilized powder of the present invention is used as an analgesic drug.

The application of the spider lyophilized powder of the invention in preparing anti-fat health wine.

The method for preparing the spider lyophilized powder of the invention has high production efficiency, small loss of active ingredients in the spider, and simple process.

detailed description

The invention is further described in the following examples, but the examples are not intended to limit the invention.

Example 1

Take the dead body of an adult tiger-shaped bird-trapping spider with a mass of about 30 grams. Rinse the spider body with water and dismember it. The spider limbs were immediately placed in a sealed plastic bag and placed in a liquid nitrogen tank for one hour, and the spider limbs were frozen. The frozen spider body was freeze-dried in a common lyophilizer after pre-cooling for 1 hour at -45 Torr, and the temperature in the lyophilizer was lowered to -50 ° C, and the pressure in the lyophilizer was controlled by vacuuming. 0. 1 standard large Air pressure, dry for 12 hours to turn off the freeze dryer. The succulent spider limb was ground in a dry environment, and the spider powder was 6.7 g, and the finished product was sealed and stored.

Example 2

Take the body of an adult godly hairy spider that has just been killed, and the quality is about 17 grams. Rinse the spider body with clean water. The spiders were immediately placed in a sealed plastic bag and chilled in a low temperature refrigerator at 70 ° C for 7 hours, and the spider corpse was frozen. The spider corpses were ground in a dry environment with a mortar to obtain spider powder. The lyophilizer is placed in a conventional lyophilizer for 1 hour after being pre-cooled for 1 hour, and the temperature in the lyophilizer is lowered to -50 ° C. The standard temperature is dried, and the lyophilizer is turned off for 8 hours. The spider powder is collected 3. 3 g, and the finished product is sealed and stored.

Example 3

Application of spider lyophilized powder in health care products:

Component and dosage: 38 degrees white spirit per 1000ml, 1 gram of lyophilized powder prepared in Example 1, 1 gram of hippocampus, 1 gram of velvet antler, 2 grams of red ginseng, 20 grams of medlar, 12 grams of Epimedium, 12 schisandra Gram.

Process: Weigh the raw materials proportionally, crush, add one-half of the white wine, shake or stir overnight, and filter. Then, the remaining one-half of the white spirit was added to the dross, and the previous operation was repeated, and the filtrate was combined. Mix with honey to taste.

The main health care function of spider health wine is anti-fatigue. After swimming in mice, the swimming time of the drug-administered group is 5 times that of the blank group, which can last up to 2 hours. The anti-fatigue effect is very significant. Example 4

Application of spider lyophilized powder in the treatment of arthritis drugs:

The spider lyophilized powder prepared in Example 1 was formulated into a 0.1% wine containing spider lyophilized powder using 38-degree white spirits. Effect test: After the rat test, the arthritis caused by the two inflammatory agents of formaldehyde and complete adjuvant, the blank group was self-healing for 15 days, and the administration group was cured for 5 days. Test method: The formaldehyde-induced inflammation method was firstly injected with 2% formaldehyde O.lrnl into the third and fourth joints of the right hind leg of the rat, resulting in arthritis. After one day, the joint swelling reached a maximum. Then, the blank group and the drug-administered group were compared. There were eight rats in each group. The drug-administered group was given 0.2 ml of the wine once a day, and the circumference of the joint (cm) and the time of disappearance of arthritis were recorded and calculated. The average results are as follows -

It can be seen that the prepared liquor has a significant effect on the treatment of arthritis.

Example 5 Application of spider lyophilized powder in analgesics: The lyophilized powder was directly used as a medicine, and a 1% spider lyophilized powder was added to a 35-degree white wine to prepare a wine.

Effect experiment: Using the mouse writhing test, eight mice were intragastrically administered with 0.2 ml of the obtained wine, and then 0.2 ml of 0.6% acetic acid was used to cause pain in the mice. Eight mice in the blank group were directly painfully treated with 0.6% acetic acid 0.2 ml, and then the number of writhing in 60 minutes was observed and calculated. The average value is as follows: The average number of writhing times of the group number sample at the earliest start of the writhing time of 60 min

1 This case begins to writh in 15 minutes.

2 blank 3 minutes to start twisting 36

Claims

A method of preparing a spider lyophilized powder according to claim 1, comprising the steps of: pretreatment, freezing, lyophilization and pulverization. 2. The method for preparing a spider lyophilized powder according to claim 1, wherein the preparation process comprises:

(1) pre-treatment

Wash the fresh spider corpse with water or dismember it;

(2) freezing

(3) Freeze-dried

In a pre-cooled lyophilizer, the frozen spider or limb is dried at -45 Torr or lower and 0.1 standard atmosphere, and the drying time is controlled to be 8-15 hours;

(4) crushing

3. The method for preparing a spider lyophilized powder according to claim 1, wherein the preparation process comprises -

(1) pre-treatment

Wash the fresh spider corpse with water or dismember it;

(2) freezing

Frozen or washed spider corpses or their limbs in liquid nitrogen or at -20 ° C and below until they are frozen; (3) crushing

Smashing the frozen spider corpse or its limbs;

(4) Freeze-dried

In a pre-cooled lyophilizer, the spider powder was dried at -45 ° C or lower and 0.1 standard atmospheric pressure, and the drying time was controlled to be 8- 15 hours, collected and sealed for storage.

4. Application of spider lyophilized powder as a medicine for treating arthritis.

5. Application of spider lyophilized powder as an analgesic drug.