CN1806485A - Acanthopanax seed propagation method - Google Patents
Acanthopanax seed propagation method Download PDFInfo
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- CN1806485A CN1806485A CN 200610003940 CN200610003940A CN1806485A CN 1806485 A CN1806485 A CN 1806485A CN 200610003940 CN200610003940 CN 200610003940 CN 200610003940 A CN200610003940 A CN 200610003940A CN 1806485 A CN1806485 A CN 1806485A
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Abstract
The invention relates to a method for wilsonii seed breeding, which is to solve problem of low rate of emergence in current technology, characterized in that it comprises the following steps: treatment of temperature-change stratification; room treatment; mixing wilsonii seed with sand before November according to ratio of 1:5-6, and then putting into culture box or culture bed with the thickness being 15-20 cm to culture for 100-120 days under 15-17 Deg. C, low temperature treating in second part of next year February, which in detail is to sift seed and put under 0-5 Deg. C for 30-40 days without being influenced by freezing of 0-minus 30 Deg. C. The rate of emergency in this invention is over 85%.
Description
Technical field:
The present invention relates to a kind of propagation method of medicinal plant seed, particularly a kind of acanthopanax seed propagation method.
Background technology:
Wilsonii belongs to Araliaceae Acanthopanax woody plant, is a kind of woody plant with precious medical value, always is China's medicine treasure, at home and abroad enjoys high reputation.What " peaceful one of slender acanthopanax, the not full car of gold and jade of getting " just arranged from ancient times says that its root, stem, Ye Junke are used as medicine.Wilsonii is widely used in psychasthenia, diabetes, arteriosclerosis clinically, rheumatism, rheumatic heart disease, cardiovascular disease, and as strong and nutrition prophylactic agent, have the function of " strengthening the body resistance to consolidate the constitution " effect of similar genseng and bowl spares benefit essence, hard muscles and bones, strong will, medical value is very high.Selling well in the medicine and the beverage industries market that with the wilsonii are raw material, is subjected to domestic and international welcome deeply.As a kind of traditional Chinese medicine with unique drug effect, the consumption of wilsonii wild resource grows with each passing day, rob over nearly twenties years formula, no plan excavates, and wild resource is seriously damaged, it is in imminent danger that its natural resources are faced, and suddenly becomes exhausted.The wilsonii propagation method has vegetative propagation (mainly based on cuttage) and two kinds of methods of sexual propagation; Sexual propagation coefficient height, growth of seedling stalwartness." yet tiger shackles; difficulty was seen seedling in 10 years ", the wilsonii seeding ratio is very low, reason is that the wilsonii seed quality is poor, resting stage is long, germination rate is low, emergence rate is not high, so the sexual propagation rate is very low, though main cause is a fruit morphological maturity, fertilized egg needs to form embryo under suitable bar, reaches physiological ripening.
Summary of the invention:
The objective of the invention is to solve the low difficult problem of existing wilsonii seeding ratio, the invention provides a kind of acanthopanax seed propagation method, the percentage of seedgermination that this method was handled is more than 85%.
The technical solution adopted in the present invention is: this acanthopanax seed propagation method, it is characterized in that: this method comprises the following steps: that alternating temperature stratification handles: indoor processing: before November wilsonii seed and sand are put into incubator or culture bed after by 1: 5～6 mixings, thickness is 15～20cm, under 15 ℃～17 ℃ temperature, cultivated 100～120 days, carry out low temperature treatment in mid-February, second, the low temperature treatment way is that seed was sifted out under the low temperature that is placed on 0～5 ℃ 30～40 days, is not afraid of between hypothermic phase 0～-30 ℃ freezing to occur.
Above-mentioned seed and the preliminary treatment of river sand: a, seed treatment: the wilsonii seed was soaked in water 2～3 days, rubs broken, water logging, rinsing with the hands, remove pericarp, pulp, flat grain, select 300 times of liquid carbendazim sterilizations of full kind grain after draining the water; B, river sand are handled: river sand sieves, and carries out disinfection with the 800ppm carbendazim aqueous solution; The ratio of seed and river sand is 1: 5～6.
This method also comprises the growth regulator processing: carrying out at the initial stage that alternating temperature stratification is handled once is that the sprinkling of the 50mg/L gibberellin aqueous solution is culture bed with concentration.
Above-mentioned alternating temperature stratification is handled by following method and substituted: natural alternating temperature stratification is handled: annual mid or late June the seed treatment that will gather last year back and the river sand of handling well are put into preprepared ditch furrow after in 1: 5～6 ratio mixing well, thickness is about 15cm～20cm, the shading of last covering 4～5cm river sand is preserved moisture, outdoor passing the winter naturally carried out the lamination processing under field conditions (factors), and early and middle ten days sifted out seed and sowed April.
The invention has the beneficial effects as follows: adopt technique scheme, in the wilsonii seed alternating temperature stratification processing procedure, temperature is to influence a principal element of germinateing after its embryo afterripening and the seed treatment.Adopt the alternating temperature tupe of low temperature behind the first high temperature to handle the wilsonii seed, between the megathermal period with greater than 15 ℃ for well, high temperature reached about 100 days, 1600～1700 ℃ of accumulative total accumulated temperature, treat to carry out low temperature treatment after the seed embryogenesis, 30～40 days lamination treatment effect of 0～5 ℃ of low temperature (not being afraid of the freezing of 0～-30 ℃ of appearance between hypothermic phase) is best, and germination rate is more than 85%.
Embodiment:
Below with the invention will be further described: this acanthopanax seed propagation method comprises the following steps: the alternating temperature stratification processing: this processing method has two kinds:
(1) indoor processing: winter, processing can be carried out under indoor conditions, before November with the wilsonii seed handled well and sand by putting into incubator or culture bed in 1: 5 or 1: 6 behind the mixing, thickness is 15cm (routine 18cm, 20cm), under the temperature of 15 ℃ (16 ℃, 17 ℃ of examples), cultivate 100 days (example 110 days, 120 days), 1600～1700 ℃ of accumulative total accumulated temperature, treat to carry out in mid-February, second after the seed embryogenesis low temperature treatment, low temperature treatment is that wilsonii is finished after-ripening and reached the necessary condition of emerging.The low temperature treatment way is sifted out the low temperature (not being afraid of the freezing of 0～-30 ℃ of appearance between hypothermic phase) that is placed on 0～5 ℃ with seed and was descended 30～40 days, and germination rate is more than 85%.Treated seed can directly be sowed.
(2) outdoor processing: annual mid or late June the seed treatment that will gather last year put into preprepared ditch furrow behind back and the river sand of handling well the ratio mixing well in 1: 5 or 1: 6, thickness is about 15cm, last covering 4cm or the shading of 5cm river sand are preserved moisture, cultivate under field conditions (factors), accumulative total accumulated temperature can reach 1600～1700 ℃ before going into the winter, embryo is reached maturity gradually, when the kind embryonic development reaches near the endosperm size, has possessed the condition of germinateing.Outdoor passing the winter naturally carried out lamination under field conditions (factors) and handled, and notes observing, and early and middle ten days sifted out seed and sowed April, has safe and reliablely, is difficult for characteristics such as mouldy, simple and easy to do, germination rate height.
Table 1 lamination processing scheme
Temperature adopts: on behalf of 15 ± 0.5 ℃ of high temperature, the tupe of high temperature-low temperature-high temperature, T handle the accumulation temperature, by T
1To T
5Carry out 1000 ~ 1200 ℃, 1200 ~ 1400 ℃, 1400 ~ 1600 ℃, 1600 ~ 1800 ℃, 1800 ~ 2000 ℃ of high temperature treatment accumulation temperature before representing low temperature treatment respectively; On behalf of low temperature, P handle fate for 0 ± 0.5 ℃, by P
1To P
5Representative was handled 10 days, 20 days, 30 days, 40 days and 50 days respectively.High temperature-low temperature-high temperature transient process is to carry out transition gradually 2 ℃ of average every days, and a wherein back high temperature treatment is carries out germination test after nature heated up 10 days, and temperature is controlled at controlled refrigerator and phytotron carries out.
Table 2 treatment of different temperature is to the influence of wilsonii percentage of seedgermination
| Handle | On average (%) | The significance of difference | |
| 0.05 | 0.01 | ||
| Handle (T 4+P 3) | 86.6667 | a | A |
| Handle (T 1+P 4) | 85 | a | A |
| Handle (T 5+P 3) | 80.6667 | a | A |
| Handle (T 1+P 2) | 76 | a | A |
| Handle (T 3+P 3) | 71.6667 | a | A |
| Handle (T 5+P 5) | 70.6667 | a | A |
| Handle (T 5+P 4) | 46 | b | B |
| Handle (T 4+P 5) | 43 | bc | B |
| Handle (T 5+P 2) | 40.6667 | bcd | B |
| First (the T of place 3+P 2) | 37.3333 | bcd | BC |
| Handle (T 3+P 4) | 32.6667 | bcde | BCD |
| Handle (T 3+P 5) | 27.6667 | bcdef | BCDE |
| Handle (T 4+P 1) | 25.6667 | cdef | BCDEF |
| Handle (T 3+P 1) | 25 | cdef | BCDEF |
| Handle (T 5+P 1) | 23.6667 | def | BCDEF |
| Handle (T 2+P 4) | 14.6667 | efg | CDEF |
| Handle (T 2+P 5) | 13.3333 | fg | CDEF |
| Handle (T 2+P 3) | 13 | fg | CDEF |
| Handle (T 2+P 2) | 12.6667 | fg | CDEF |
| Handle (T 2+P 1) | 8.3333 | fg | DEF |
| Handle (T 1+P 5) | 2.3333 | g | EF |
| Handle (T 1+P 4) | 2 | g | F |
| Handle (T 1+P 3) | 1.6667 | g | F |
| Handle (T 1+P 2) | 0.3333 | g | F |
| Handle (T 1+P 1) | 0 | g | F |
For make germination rate higher be preferably in to seed carry out alternating temperature stratification handle before to the preliminary treatment of carrying out of seed and river sand: seed treatment: the wilsonii seed of the wild wilsonii seed natural seasoning that will gather (the wild wilsonii seed of collection), be soaked in water 2 or 3 days, the interior stranding of the woven bag of packing into is broken, pericarp, pulp, flat grain are removed in the water logging rinsing, select 300 times of liquid carbendazim sterilizations of full kind grain after draining the water; River sand is handled: river sand sieves, and carries out disinfection with the 800ppm carbendazim aqueous solution.The ratio of seed and river sand was generally 1: 5 or 1: 6.
More handle to cultivating height of bed growth regulator that to carry out at the initial stage that alternating temperature stratification is handled once be 50mg/L gibberellin (GA with concentration for making germination rate
3) aqueous solution spray culture bed, handle the wilsonii seed with plant growth regulator after germination rate have tangible raising, this also is one of success factor of handling of alternating temperature stratification.
The different plant growth regulator treatment combinations of table 3 are to the influence (%) of wilsonii percentage of seedgermination
| GA 3+KT(mg/L) | Average (%) Average (%) | The F test | |
| 0.05 | 0.01 | ||
| Processing 11 (50+0) processing 13 (50+50) processing 12 (50+25) processing 14 (50+75) processing 17 (75+25) processing 23 (100+50) processing 18 (75+50) processing 19 (75+75) processing 22 (100+25) processing 21 (100+0) processing 24 (100+75) processing 15 (50+100) processing 20 (75+100) processing 16 (75+0) processing 9 (25+75) processing 25 (100+100) processing 10 (25+100) processing 8 (25+50) processing 5 (0+100) processing 6 (25+0) processing 3 (0+50) processing 4 (0+75) processing 7 (25+25) processing 2 (0+25) processing 1 (0+0) | 88.0 72.0 70.3 62.0 55.3 48.3 40.8 47.7 46.7 46.3 44.0 43.3 41.7 40.7 37.3 34.7 32.0 29.7 24.7 21.3 20.7 18.7 16.7 11.3 2.3 | a ab ab bc bcd cde cde cdef cdef cdef cdefg cdefgh cdefgh cdefghi defghij defghij efghijk efghijk fghijk ghijkl hijkl ijkl jkl kl l | A AB ABC ABCD BCDE BCDEF BCDEF BCDEF BCDEFG BCDEFG BCDEFG BCDEFG CDEFG CDEFGH DEFGH DEFGH DEFGH EFGHI FGHI FGHI FGHI FGHI GHI HI I |
In addition, the wilsonii seed utilizes low concentration gibberellin (50ppm GA after handling through alternating temperature stratification
3The surface of the seed sprinkling infiltration before broadcasting) handles effectively stratification and bud elongate axis.But concentration is unsuitable too high, otherwise causes that easily radicle is too very thin, and seedling resistance is poor, is unfavorable for cultivating strong sprout.
It is that the black ripe back of acanthopanax fruit fertilized egg is vesicular follicle that the wilsonii seed has long resting stage.Be deep in the endosperm, endosperm epidermal cell wall is a fatty matter institute suberification, has constituted an impermeable stratum.Has the function of isolating or cushioning the moisture turnover.Fertilized egg just germinates under room temperature condition in the black ripe back of fruit.Wilsonii sexual propagation is a kind of simple and easy to do, effective ways that reproduction rate is high.But owing to plant the embryonic development restriction, seldom can see the natural seedling of wilsonii under field conditions (factors).Adopt suitable alternating temperature stratification treatment temperature condition, can significantly promote the afterripening of wilsonii seed, accelerate kind of an embryonic development, improve germination rate.
The conventional way of breaking the full seed dormancy is that normal temperature mixes the long-pending processing of sand bed, treat that its physiology after-ripening directly sows, it is unsuccessful that this method is handled wilsonii, and emergence rate is very low, germination rate and emergence rate after the processing have only 5%～10%, are difficult to use on producing.Having taked the alternating temperature stratification processing at this case study, is exactly that carry out seed: sand was 1: 3～5 the mixed sand of ratio, carries out alternating temperature stratification and handles after seed was handled with plant growth regulator.
The development condition of routine observation seed embryo during alternating temperature stratification is handled, during wilsonii seed results, embryo does not have zoon and can't see embryo, need the alternating temperature stratification after-ripening of finishing dealing with, the seed embryo forms gradually during lamination, along with the increase embryo of lamination accumulated temperature is reached maturity gradually, when the kind embryonic development reaches near the endosperm size, possessed the basic condition that germinates, but must also will have the vernalization of low temperature to germinate.A series of variation takes place in the seed body after the wilsonii after-ripening is finished, and wherein the formation of polyoses grain is main, and can sprout seed be necessary, and the formation of polyoses grain and the cyclically-varying of temperature have direct relation.
Claims (4)
1, a kind of acanthopanax seed propagation method is characterized in that: this method comprises the following steps:
Alternating temperature stratification is handled: before November wilsonii seed and sand are put into incubator or culture bed after by 1: 5～6 mixings, thickness is 15～20cm, under 15 ℃～17 ℃ temperature, cultivated 100～120 days, carry out low temperature treatment in mid-February, second, the low temperature treatment way is that seed was sifted out under the low temperature that is placed on 0～5 ℃ 30～40 days.
2, acanthopanax seed propagation method according to claim 1, it is characterized in that: alternating temperature stratification is handled preceding preliminary treatment to seed and river sand: a, seed treatment: the wilsonii seed was soaked in water 2～3 days, rub broken, water logging, rinsing with the hands, remove pericarp, pulp, flat grain, select 300 times of liquid carbendazim sterilizations of full kind grain after draining the water; B, river sand are handled: river sand sieves, and carries out disinfection with the 800ppm carbendazim aqueous solution.
3, acanthopanax seed propagation method according to claim 2 is characterized in that: also comprise the growth regulator processing: carrying out at the initial stage that alternating temperature stratification is handled once is that the sprinkling of the 50mg/L gibberellin aqueous solution is culture bed with concentration.
4, acanthopanax seed propagation method according to claim 1, it is characterized in that: alternating temperature stratification is handled by following method and substituted: natural alternating temperature stratification is handled: annual mid or late June the seed treatment that will gather last year put into preprepared ditch furrow behind back and the ratio mixing of river sand of handling well well in 1:5～6, thickness is about 15cm～20cm, the shading of last covering 4～5cm river sand is preserved moisture, outdoor passing the winter naturally carried out the lamination processing under field conditions (factors), and early and middle ten days sifted out seed and sowed April.
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| CNB2006100039401A CN100403876C (en) | 2006-01-23 | 2006-01-23 | Acanthopanax seed propagation method |
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|---|---|---|---|
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Cited By (9)
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|---|---|---|---|---|
| CN101341817B (en) * | 2008-08-22 | 2010-12-29 | 东北林业大学 | Method for improving germination rate of aralia mandshrica seed |
| CN102077751A (en) * | 2010-10-29 | 2011-06-01 | 屈国良 | Artificial domestication out-of-season planting technology of vegetable acanthopanax senticosus |
| CN103283341A (en) * | 2013-05-24 | 2013-09-11 | 河北科技师范学院 | Method for promoting hawthorn seed kernel to germinate ahead of time |
| CN104012278A (en) * | 2014-06-12 | 2014-09-03 | 吉林农业大学 | Manyprickle acanthopanax root intermittent seed germination accelerating and autumn seedling culture method |
| CN104996124A (en) * | 2015-06-29 | 2015-10-28 | 吉林省弘宸科技有限公司 | Cultivation method for acanthopanax |
| CN105557443A (en) * | 2016-03-03 | 2016-05-11 | 杨友仁 | Method for planting radix et caulis acanthopanacis senticosi in forest lands in northern China |
| CN105900559A (en) * | 2016-04-20 | 2016-08-31 | 中国中医科学院中药研究所 | Method for promoting seed germination of manyprickle acanthopanax |
| CN107736196A (en) * | 2017-11-02 | 2018-02-27 | 昆明功德生物科技有限公司 | A kind of preparation method of purple radix Acanthopanacis senticosi tea |
| CN108419475A (en) * | 2018-03-21 | 2018-08-21 | 黑龙江中医药大学 | A kind of method that endogenetic fungus promotes wilsonii germination |
Family Cites Families (1)
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| CN1181723C (en) * | 2002-10-22 | 2004-12-29 | 黑龙江省沙河农场 | Sexual propagation method for wilsonii |
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- 2006-01-23 CN CNB2006100039401A patent/CN100403876C/en not_active Expired - Fee Related
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101341817B (en) * | 2008-08-22 | 2010-12-29 | 东北林业大学 | Method for improving germination rate of aralia mandshrica seed |
| CN102077751A (en) * | 2010-10-29 | 2011-06-01 | 屈国良 | Artificial domestication out-of-season planting technology of vegetable acanthopanax senticosus |
| CN103283341A (en) * | 2013-05-24 | 2013-09-11 | 河北科技师范学院 | Method for promoting hawthorn seed kernel to germinate ahead of time |
| CN103283341B (en) * | 2013-05-24 | 2014-08-13 | 河北科技师范学院 | Method for promoting hawthorn seed kernel to germinate ahead of time |
| CN104012278B (en) * | 2014-06-12 | 2015-11-04 | 吉林农业大学 | A kind of wilsonii next year presprouting of seeds and autumn nursery technique method |
| CN104012278A (en) * | 2014-06-12 | 2014-09-03 | 吉林农业大学 | Manyprickle acanthopanax root intermittent seed germination accelerating and autumn seedling culture method |
| CN104996124A (en) * | 2015-06-29 | 2015-10-28 | 吉林省弘宸科技有限公司 | Cultivation method for acanthopanax |
| CN104996124B (en) * | 2015-06-29 | 2017-08-25 | 吉林省弘宸科技有限公司 | A kind of cultural method of wilsonii |
| CN105557443A (en) * | 2016-03-03 | 2016-05-11 | 杨友仁 | Method for planting radix et caulis acanthopanacis senticosi in forest lands in northern China |
| CN105900559A (en) * | 2016-04-20 | 2016-08-31 | 中国中医科学院中药研究所 | Method for promoting seed germination of manyprickle acanthopanax |
| CN105900559B (en) * | 2016-04-20 | 2018-12-07 | 中国中医科学院中药研究所 | A method of promote wilsonii seed to sprout |
| CN107736196A (en) * | 2017-11-02 | 2018-02-27 | 昆明功德生物科技有限公司 | A kind of preparation method of purple radix Acanthopanacis senticosi tea |
| CN108419475A (en) * | 2018-03-21 | 2018-08-21 | 黑龙江中医药大学 | A kind of method that endogenetic fungus promotes wilsonii germination |
| CN108419475B (en) * | 2018-03-21 | 2020-12-18 | 黑龙江中医药大学 | Method for promoting germination of acanthopanax seeds by endophytic fungi |
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