CN1798761A - 肠球菌抗原 - Google Patents
肠球菌抗原 Download PDFInfo
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- CN1798761A CN1798761A CNA2004800151595A CN200480015159A CN1798761A CN 1798761 A CN1798761 A CN 1798761A CN A2004800151595 A CNA2004800151595 A CN A2004800151595A CN 200480015159 A CN200480015159 A CN 200480015159A CN 1798761 A CN1798761 A CN 1798761A
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Abstract
本发明公开了来自粪肠球菌的编码超免疫血清反应性抗原或其片段的分离的核酸分子以及超免疫血清反应性抗原或其片段、分离这样的抗原的方法及其特定用途。
Description
本发明涉及编码肠球菌,例如粪肠球菌(Enterococci faecalis)抗原的分离的核酸分子,所述分子适合用于预防和治疗肠球菌引起的细菌感染的药物药剂的制备。
肠球菌是革兰氏阳性细菌,其为人和动物消化道中正常的聚居者。一个世纪以来其业已被认为是传染性心内膜炎的原因{Murray,B.,1990},且由于作为医源性感染以及手术部位感染的最常见病原体在过去二十年来受到高度重视{Richards,M.等人,2000}。肠球菌作为医源性病原体的日益重要部分归因于其固有的和获得性的抗生素抗性{Murray,B.,1990};{Rice,L.,2001}。多药抗性肠球菌的治疗对临床医生提出了有力地挑战。{Cetinkaya,Y.等人,2000};{Gold,H.,2001},且这类生物作为抗生素抗性基因的潜在储藏库尤其令人关注{French,G.,1998};{Noble,W.等人,1992};{Poyart,C.等人,1997}。
肠球菌作为D组链球菌的分类追述到1930年代早期。1984年,在数项研究证实与链球菌的远缘关系后被给出了正式的种属名称分类。肠球菌通常被认为是多种生物(包括人)胃肠道中的共生体。尽管有多于14种的不同肠球菌,粪肠球菌(E.faecalis)和屎肠球菌(E.faecium)是人类中最常见的种类。肠球菌数种固有特征使得所述种属的成员存活更长的时间,造成其存留和医源性传播。肠球菌在去污剂存在下的适应和存留的能力允许其在不充分的清洁方案中存活,造成其在医院的存留。肠球菌固有的粗放性也赋予其对包括氨基糖苷类、β-内酰胺类和喹诺酮类的数种抗生素的不同寻常的耐受水平。例如,肠球菌对氨基糖苷类的耐受源自肠球菌对药物在细胞壁处摄入的阻断。尽管高水平抗性的机制被确定为双功能酶的结果,{Ferretti,J.等人,1986},肠球菌对低水平氨基糖苷类的内在抗性机制仍待确定。
在肠球菌中,屎肠球菌是独特的,因为其通常用于制备发酵食品,且用作益生菌。近年来,屎肠球菌作为食品发酵微生物不甚被接受,因为担心该细菌可作为向参与人类感染的细菌传递抗生素抗性的中间体宿主。尽管存在上述担心,屎肠球菌仍是乳酸菌发酵的食品中最常见的细菌。已证实许多屎肠球菌的分离物产生杀细菌素(抗微生物肽),其能够杀死或抑制病原体(如李斯特菌属,梭菌属,杆菌属和葡萄球菌)的生长。这类杀菌素有助于食品发酵的保藏作用,且是所述菌株作为发酵食品生产的出发培养物的一个原因。近来,肠球菌已被成功地用于牛乳腺炎的治疗。
除了食品生产、作为益生菌以及在动物疾病治疗中的应用,最重要的,肠球菌是新出现的人条件病原体。这是由于其固有的病原性潜能,甚至是因为其快速获得抗生素抗性基因的能力。屎肠球菌和粪肠球菌是高百分比的医院获得性感染(包括超级感染)的原因。
肠球菌通常聚集在人类胃肠道中。在人类粪便中较丰富。在肠球菌及其宿主中很可能存在密切相关,否则由于正常的肠道运动所述微生物将消失。许多感染来源的肠球菌分离株为克隆性的,显示医源性传播。此外,许多研究表明在住院后出现患者群居,显示多种抗性菌株的群居是继发感染的易感因子。
医源性肠球菌感染的不易解释之谜之一是在相同种属成员业已占据的生态环境中的容易群居。如前述,缺乏实质上抗肠球菌活性的抗生素(即,对土著肠球菌不具有毒害作用的抗生素)是感染的重要易感因子。所述感染常常由多种抗性肠球菌分离株引起,所述分离株已被外源性获得,且在没有定向选择的情况下显示优于土著肠球菌。
外源性、多重抗性、医源性传播的肠球菌有效群居在胃肠道的事实提示其可能不是直接竞争土著菌株的同一小生态环境。
由肠球菌属引起的感染包括a)菌血症,b)尿道感染,c)眼内炎,d)心内膜炎,以及创伤和腹内感染。大约3/4的感染由粪肠球菌引起,其余由屎肠球菌引起。
a)菌血症
1986年10月-1997年4月间医院监护数据将肠球菌列为第三位最常见的医源性菌血症的病因,占所有分离株的12.8%。肠球菌跨越肠上皮细胞屏障的转位被认为导致了许多无可鉴别来源的菌血症。与肠球菌菌血症的死亡率相关的风险因子包括疾病的严重程度,患者年龄以及广谱抗生素的使用,如第三代头孢类抗生素或甲硝唑。这些研究表明,高水平氨基糖苷类抗性不影响临床结果,但是,粪肠球菌溶细胞素(溶血素)的存在可增强感染的严重程度。
b)尿道感染
据估计肠球菌每年在美国造成110,000例尿道感染(UTI)。一些研究业已致力于了解肠球菌与尿路上皮组织的相互作用。已证实质粒编码的聚集性物质在肠球菌与肾上皮细胞吸附中的潜在作用。携带外激素应答性质粒pAD1或其同源基因衍生物的粪肠球菌比不含质粒的细胞能够更好的结合于培养的猪肾小管细胞系LLC-PK。他们的研究结果显示含有纤连蛋白基序Arg-Gly-Asp-Ser的合成肽能够抑制结合。所述结构性基序介导纤连蛋白与整合素家族真核表面受体的相互作用。
c)眼内炎
宿主组织的聚居在眼内炎的发病中起重要作用。肠球菌作为最具有破坏性因子之一引起白内障手术的手术后并发症。设计用于确定是否靶定粪肠球菌的物质改变眼内解剖结构的实验表明,肠球菌附着于玻璃体的膜结构,但是所述吸附不依赖于聚集物质是否存在。
d)心内膜炎
在肠球菌引起的多种感染中,传染性心内膜炎(IE)是最具有治疗挑战性的之一。肠球菌是传染性心内膜炎的第三位致病因素,占天然瓣膜IE病例中的5-20%,人工瓣膜心内膜炎的6-7%。外激素应答性质粒pAD1的存在增强了肠球菌性心内膜炎的“植被类型”(vegetationformation)。来自带有粪肠球菌的心内膜炎患者的血清用于鉴别在血清中选择性表达而不在培养基中表达的抗原{Lowe,A.等人,1995}。所述蛋白质抗原称为EfaA,具有与数种肠球菌粘附相似的广泛序列,且可能作为重要的心内膜炎吸附素。
氨苄青霉素是肠球菌感染的治疗选择。对于严重肠球菌感染,特别是心内膜炎,氨基糖苷类作为与青霉素或氨苄青霉素组合治疗的一部分非常重要。尽管肠球菌固有的耐受低水平的氨基糖苷类抗生素,针对氨基糖苷类的细胞壁抑制剂的添加导致通过两种抗生素协同作用增加的杀伤。随着对氨基糖苷类和青霉素类高水平抗性的增加,万古霉素成为可用于肠球菌感染的唯一选择。随后,1988年在肠球菌临床分离株中报道了万古霉素抗性,随后是由万古霉素抗性肠球菌(VRE)引起的爆发。在美国医院中,医源性肠球菌对万古霉素抗性的百分比从1989年的0.3%增加到1993年的7.9%。在重症监护室中患有医源性感染的患者中,增加更为明显:从1989年的0.4%至1993年的13.6%,在四年期间增加了34倍。
除了较高的死亡率之外,万古霉素抗性肠球菌感染造成平均约$25,000的额外治疗费用,同时延长了一倍的患者住院时间。
尤其是在粪肠球菌分离株中万古霉素抗性的急剧增加,表明感染在未来提出越来越多的挑战。一直显见的治疗替代方案是接种疫苗,旨在诱导防止或减轻感染的保护性免疫反应。
由于缺乏关于针对肠球菌感染的保护性免疫元件的足够知识,疫苗的开发受到阻碍。有报道称中性粒细胞介导的肠球菌杀伤是对抗体的主要补充,所述抗体起到不甚关键但是潜在的重要作用,尽管最近报道了关于抗肠球菌抗体通过调理吞噬杀伤在促进清除中的重要性之额外证据{Gaglani,M.等人,1997}。
针对肠球菌的人免疫中表面蛋白的重要性也已知晓。似乎所有的临床分离株表达具有与宿主免疫防御相关的活性之表面蛋白。肠球菌表面蛋白(Esp){Shankar,V.等人,1999},明胶酶,溶细胞素{Haas,W.等人,2002}和聚集物质(AS)表面蛋白{Sussmuth,S.等人,2000}已被生物化学和遗传学充分表征,显示为免疫原性的{Xu,Y.等人,1997}。在传染性心内膜炎的动物模型中,针对聚集物质的特定抗体仍不是保护性的{McCormick,J.等人,2001}。
因此,仍旧需要预防和缓解肠球菌感染的有效治疗。能够显示针对引起人类感染的主要肠球菌菌株的交叉保护作用之疫苗可用于防止或缓解由所有其他肠球菌种属,即粪肠球菌和屎肠球菌引起的感染。
疫苗能含有全部的各种不同的抗原。抗原的实例是完全杀死的或减毒的生物体、这些生物体/组织的亚组分、蛋白质或者它们最简单的形式肽。抗原也能以糖基化蛋白质或肽的形式被免疫系统识别,也可以是或含有多糖或脂质。能使用短肽,因为例如细胞毒性T细胞(CTL)识别连接于主要组织相容性抗原(MHC)的通常8-11个氨基酸长的短肽形式的抗原。B细胞能识别短到4-5个氨基酸的线性表位以及三维结构(构象表位)。为了获得持续的抗原特异性免疫反应,佐剂需要激发包括免疫系统所有细胞的免疫级联。起初,佐剂起作用,但是不限于它们对所谓抗原递呈细胞(APC)的作用的模式。这些细胞通常最先遇到抗原,之后是加工的或未修饰的抗原呈递给免疫效应细胞。也可以涉及中间细胞类型。只有有适当特异性的效应细胞在生产免疫反应中被激活。佐剂也可以局部保留抗原和共注射的其他因子。另外,佐剂可以起其它免疫细胞的化学趋化剂的作用,或者可以在局部和/或全身起免疫系统刺激剂的作用。
目前,针对肠球菌的疫苗仅处于开发的研究阶段。研究不仅致力于荚膜多糖(CPS)作为免疫原(Hueber,J.等人,2000),也致力于毒力因子和膜/表面蛋白。
蛋白质偶联疫苗的开发无疑是抗肠球菌疾病战斗增加的一个伟大的新武器,但是疫苗仅含有有限数量的肠球菌蛋白质,给与足够的生态学压力,致病源变化及非疫苗临床分离株的质粒仍然是真正的威胁。此外用于活性免疫的多糖抗原在人类中不提供免疫记忆。CPS与非肠球菌相关免疫原性蛋白质载体结合(例如,破伤风类毒素、霍乱毒素B亚单位等等)已经显示在诱导疫苗接种者更高的抗体浓度是有利的,但是它不提供在真正的感染过程中招募记忆B和T细胞以支持最有效宿主反应的病原体特异性B细胞和T细胞表位。为了能够用符合这些标准的蛋白质补充肠球菌疫苗,鉴别保守免疫原性肠球菌特异性表面蛋白质是必须的。
基于被动抗体的治疗有很大的潜力。为了预防已经尝试使用人类静脉内免疫球蛋白(IVIG)的制备物。单克隆抗体生产技术的最新进展提供了产生人类抗体试剂和再引入抗体治疗的方法,而避免与血清治疗相关的毒性。免疫球蛋白是极端多样类型的抗微生物蛋白质,能用于预防和治疗出现的感染疾病。抗体治疗抵抗各种不同的微生物是有效的({Burnie,J.等人,1998}的综述)。抗肠球菌的单克隆抗体能治疗性地给予每一个由于器官移植、癌症、HIV感染和其他原因的免疫抑制患者。
展示在显著造成疾病发生的革兰氏阳性生物体表面上的某些蛋白质或酶参与了这些病原体引起的疾病过程。通常,这些蛋白质参与了与宿主组织直接的相互作用或隐藏细菌表面以逃脱宿主防御机制{Navarre,W.等人,1999}。粪肠球菌在这点上并不例外。一些表面蛋白质作为对肠球菌致病性重要的毒力因子已被鉴别,综述见{Jett,B.等人,1994}。如果抗这些蛋白质的抗体对人类能提供比多糖更好的保护,它们能提供新型基于蛋白质的肠球菌疫苗的来源以联合或替代更加传统的荚膜多糖疫苗而使用。一些作为可能疫苗的抗原以及许多额外候选的上面描述的蛋白质的用途主要由基于鉴别的容易或可获得的机会的挑选产生。以更加全面的方式鉴别粪肠球菌相关抗原是有需求的。
本发明者已经开发了从特异性病原体尤其是从金黄色葡萄球菌(Staphylococcus aureus)和表皮葡萄球菌(Staphylococcusepidermidis)鉴别、分离和生产超免疫血清反应性抗原的方法(WO02/059148)。然而,考虑到生物特性、致病功能和遗传背景的不同,粪肠球菌与葡萄球菌株截然不同。为了鉴别相关血清来源,从健康成人以及从患有肠球菌感染的患者和幼稚个体(5月龄-10月龄之间的年幼儿童,在其丧失母体抗体之后,作为阴性对照)中收集三种主要类型的人血清。将高百分比的个体暴露于能够在宿主中诱导抗体之环境中的肠球菌。主要在医院中发生的疾病可能与低水平的针对肠球菌的特异性抗体相关,因此不甚有效被吞噬细胞性消除。为了能够挑选合适的筛选剂,用细菌裂解物和培养上清蛋白质进行了测量抗粪肠球菌IgG抗体水平的一系列ELISA和免疫斑点实验。来自高滴度个体的血清包括在基于基因组的抗原鉴别。
两个细菌种-粪肠球菌和金黄色葡萄球菌的基因组本身显示了许多重要的不同。粪肠球菌的基因组含有大约3.22Mb,而金黄色葡萄球菌有2.85Mb。它们分别具有37.5%和33%的平均GC含量,两个病原体之间大约1/3的编码基因不同。另外,对于增殖两个细菌种类需要不同的生长条件和培养基。两个病原体造成的最重要疾病的列表显示如下。金黄色葡萄球菌主要引起医院的机会性感染:脓疱病、毛囊炎、脓肿、疖子、感染的伤口、心内膜炎、脑膜炎、脓毒性关节炎、肺炎、骨髓炎、皮肤烫伤综合症(SSS)、毒性休克综合症。粪肠球菌主要引起根据多数测量认为不是高度毒性、高度侵入性或高度传染性的感染。尽管如此,其仍引起相当多的人类疾病,如菌血症、尿路感染、心内膜炎和腹腔内感染。
粪肠球菌V583(万古霉素抗性临床分离株)的完整基因组序列通过随机鸟枪法测序方法测定(GenBank关于染色体和质粒的登录号如下:AE016830(染色体),AE016833(pTEF1),AE016831(pTEF2),AE016832(pTEF3));见www.tigr.org/tigrscripts/CMR2/CMRHomePage.spl).{Paulsen,I.等人,2003}。
测定了带有预期基因组大小为2.8Mbp的粪肠球菌菌株DO(ATCC BAA-472,TEX16,TX0016)的完整基因组序列。该基因组目前在Baylor College of Medicine′s人类基因组测序中心和University ofTexas Center病原体出现与再出现研究组(CSERP)进行计算机处理和标注;见:www.hgsc.bcm.tmc.edu/microbial/Efaecium/。
本发明潜在的难题是提供开发例如抗粪肠球菌感染疫苗的药剂的方法。更加特别地,所述难题需提供能用于生产所述药剂的一套有效、相关和全面的来自粪肠球菌的核酸分子或超免疫血清反应性抗原。
所以,本发明提供了编码高免疫反应血清反应抗原或其片段的分离的核酸分子,包含如下核酸序列,选自
a)与选自Seq ID No 1-2,4-8,10,12-18,20-23,25-26,29-43,45-62,64-74,76-77,79-83,85-89,91-92,94-114,117-126,128-146,148-170,373,375,379-381,387,392,394,397-399,407-408,410-411和415-424的核酸分子至少具有70%序列相同性的核酸分子,
b)互补于a)的核酸分子的核酸分子,
c)包含a)或b)的核酸分子至少15个连续碱基的核酸分子
d)在严格杂交条件下退火于a)、b)或c)的核酸分子的核酸分子
e)由于遗传密码的简并性,否则将杂交于a)、b)、c)或d)中定义的核酸分子的核酸分子。
根据本发明的优选实施方案,序列相同性是至少80%,优选地至少95%,尤其是100%。
此外,本发明提供了编码超免疫血清反应性抗原或其片段的的分离的核酸分子,包含如下核酸序列,选自
a)与选自Seq ID No 3,9,11,24,27,44,63,75,84,115-116,127,374,376-378,382-386,388-391,393,395-396,400-406,409和412-414,的核酸分子至少具有96%或高于96%,优选地至少98%,尤其是100%序列相同性的核酸分子,
b)互补于a)的核酸分子的核酸分子,
c)包含a)或b)的核酸分子至少15个连续碱基的核酸分子
d)在严格杂交条件下退火于a)、b)或c)的核酸分子的核酸分子
e)由于遗传密码的简并性,否则将杂交于a)、b)、c)或d)中定义的核酸分子的核酸分子。
根据另一个方面,本发明提供了编码超免疫血清反应性抗原或其片段的分离的核酸分子,包含如下核酸序列,选自
a)选自Seq ID No 90,147的核酸分子,
b)互补于a)的核酸的核酸分子,
c)由于遗传密码的简并性,否则将杂交于a)、b)、c)或d)中定义的核酸分子的核酸分子
优选地,核酸分子是DNA或RNA。
根据本发明优选的实施方案,核酸分子从尤其是粪肠球菌基因组DNA的基因组DNA分离。
根据本发明,提供了包含根据本发明的任一核酸分子的载体。
在优选实施方案中,载体经改造以适于根据本发明的核酸分子编码的超免疫血清反应性抗原或其片段的重组表达。
本发明也提供了包含根据本发明的载体的宿主细胞。
根据另一方面本发明进一步提供了包含根据本发明的核酸分子编码的氨基酸序列的超免疫血清反应性抗原。
优选的实施方案中,氨基酸序列(多肽)选自Seq ID No 171-172,174-178,180,182-188,190-193,195-196,199-213,215-232,234-244,246-247,249-253,255-259,261-262,264-284,287-296,298-316,318-340,425,427,431-433,439,444,446,449-451,459-460,462-463和467-476。
另一优选实施方案中氨基酸序列(多肽)选自Seq ID No 173,179,181,194,197,214,233,245,254,285-286,297,426,428-430,434-438,440-443,445,447-448,452-458,461和464-466。
进一步优选的实施方案中,氨基酸序列(多肽)选自Seq ID No260,317。
根据另一方面,本发明提供了选自包含表1a和表1c“推定的免疫原性氨基酸”和“鉴别的免疫原性区域位置”纵栏的氨基酸序列的超免疫血清反应性抗原之片段,表2的血清反应性表位,尤其是包含选自如下氨基酸的肽:4-10,14-21,30-36,59-68,77-82,87-93,96-105,112-121,125-133,135-141,150-162,164-183,192-203,207-213,215-226,228-234,241-247,250-285,302-308和135-148位的Seq ID No 171;15-57,60-73,77-101,108-134,136-177,185-201,203-217,226-240,244-254,272-277,283-288,292-343,354-370,380-398,406-437,439-453,473-490,532-538,584-590,595-601,606-612,664-677,679-704,715-724,731-753,759-772,786-794,814-862和657-684位的Seq ID No 172;4-9,15-36,41-47,54-60,75-81,114-120,131-146,152-158,174-182,194-202,208-215,218-226,255-271,276-285,290-295,302-311,318-328,330-344,352-359,365-377,388-395,398-405,426-432,439-449,455-500,505-513,531-537,542-552,554-561,587-595,606-612,718-734,763-771,775-782,792-801,805-812,822-828,830-843,849-863,876-894,905-911,919-926,935-947,949-958,968-979,1009-1016,1029-1045,1047-1056,1076-1081,1092-1106,1123-1133,1179-1200,1202-1211,1215-1223,1287-1299,1301-1306,398-431和1224-1237位的Seq ID No 173;17-47,74-80,90-97,126-133,137-148,167-173,179-185,214-223,250-255,270-283,329-338,342-350,352-358,360-367,372-383,398-404,411-421,426-432,435-446,452-462,472-479,515-521,582-592,611-618,623-629,642-659,666-673,678-689,704-725,732-737,744-757,768-789,824-834,842-849,862-868,877-887,904-916,923-928,941-947,962-974,982-992,1019-1030,1032-1044,1046-1052,1065-1075,1077-1087,1108-1121,1124-1132,1137-1151,1170-1182,1190-1206,1208-1214,1227-1233,1242-1251,1254-1273,1282-1298和792-825位的Seq ID No 174;19-31,39-67,82-91,104-110,113-128,149-155,161-181和137-155位的Seq ID No 175;6-18,54-63,69-85,110-127,142-156,158-167,169-211,238-246,248-257,276-311,339-349,371-380,385-391,394-403,421-438,451-456,483-489和449468位的Seq ID No 176;5-15,24-34,50-56,61-83,98-121,123-136,149-162,166-194,202-215,221-227,229-332,337-360,367-402,404-415,427-433,444-462,471-478,487-498,511-518,521-544,550-563,568-574,580-587,597-607,610-616,624-629和468-498位的Seq ID No 177;11-19,32-49,57-63,65-71,80-89,91-133,166-181,183-191,201-230,234-257,264-291,297-303,305-314,316-335,337-354,359-366,368-374,383-388,394-405,408-442,446-470,483-490,499-505,513-538,544-555,557-563,568-590,598-608,617-623,627-636,641-647,667-685,687-693,710-723,733-739,742-754,769-815和366-388位的Seq ID No 178;4-16,30-35,42-53,67-76,82-87,101-108,112-130,132-138,147-152,161-183,187-208,218-225,265-281,295-303,305-317,322-334,338-357,360-368,370-383,387-394,400-419,421-430和255-336位的Seq ID No 179;19-27,36-47,59-66,76-83,101-112,118-125,142-147,162-180,185-196,225-240,246-263,286-304,314-319,327-333,353-367和194-214位的Seq ID No 180;14-43,70-76,83-89,111-117,122-128,136-145,163-170,175-182,210-219,246-251,266-279,325-331,338-346,348-354,356-363,368-379,422-428,431-441,450-456,466-473,509-515,532-542,549-556,576-586,605-612,617-623,636-653,660-667,674-686,698-719,726-731,738-745,762-783,818-828,836-843,856-862,871-881,903-910,917-922,935-941,956-968,976-986,1013-1024,1026-1038,1059-1069,1071-1081,1102-1115,1118-1126,1131-1145,1164-1176,1187-1200,1202-1208,1221-1227,1236-1245,1248-1267,1273-1292,252-287和805-844位的Seq ID No 181;4-18,21-28,37-43,56-70,101-113,131-140,142-150,162-170,172-184,193-204,209-227,233-238,246-264和93-168位的Seq ID No 182;14-20,44-50,61-70,77-96,99-106,129-142,168-181,187-196,205-221,225-241,277-296和257-281位的Seq ID No 183;18-29,43-54,64-76,78-84,88-103,125-149,159-176,198-218,230-242,256-271,279-285,287-293,300-306,325-331,344-351,357-364,371-397,400-414,419-464,485-515,517-526,529-537,548-553,573-580,584-590,603-620,639-661,676-681,687-700,716-761,772-780,785-790,795-803,823-836,848-853和106-134位的Seq ID No 184;7-13,19-42,44-51,55-75,87-97,99-110,112-118,129-135,141-156,158-178,213-220,230-286,294-308,323-338,345-352,355-365,370-392,394-419,437-446,454-460,474-497,515-526,528-546,569-575和128-141位的Seq ID No 185;12-20,24-33,45-70,73-84,86-94,103-116,118-124,135-142,163-170,176-200,202-224,226-234,237-248,250-262,265-287,296-307,334-341,347-356,361-369,382-396,405-415,418-427,431-439,443-449,452-461,467-474和113-146位的Seq ID No 186;13-38,44-50,52-59,66-72,83-94,103-110,116-124,131-137,158-180,199-204,218-233,241-264,269-317,326-342,350-356和70-86位的Seq ID No 187;29-35,49-59,63-84,86-97,103-111,113-126,130-144,150-158,174-198,221-231,250-264,266-273,291-298,310-318和70-90位的Seq ID No 188;19-25,28-52,60-66,71-76,131-142,149-155,157-178,181-213,218-223,237-242,250-257,260-266,272-279,282-290,321-330,373-385,393-407,441-453,461-475,509-521,529-542,577-589,597-610,643-655,663-677,703-718,729-734,358-464,495-570和604-685位的Seq ID No 189;4-29,51-76,116-136,158-173,179-193,207-215和86-111位的Seq ID No 190;5-23,45-70,79-90,93-107,114-122,142-151和18-36位的Seq ID No 191;9-51,68-120,133-149,158-180,186-206,211-220,222-237,248-293,296-310,317-339和248-260位的Seq ID No 192;14-24,44-63,69-98,108-119,123-136,155-161,164-176,180-193,203-208,215-223,239-247,274-281,283-289,296-304,306-313,315-327,331-341,343-353,357-386,392-405和205-246位的Seq ID No 193;5-13,16-23,36-42,53-63,70-83,96-102和14-34位的Seq ID No 194;4-13,19-35,49-56,59-76,83-107,121-134,144-153,157-164,166-186,194-202,209-216,231-253,257-264和98-134位的Seq ID No 195;16-32,38-47,58-68,78-89,98-114,117-123,132-141,146-156,164-170,179-188,196-212,219-230,232-237,244-263,265-274,278-293,297-303,306-326,339-349,352-359,362-367,373-379,384-394,396-406,423-443,451-461,465-484,490-497,504-511,523-533,537-547,550-556,558-566,573-579,586-593,598-609,615-642,647-665,671-686,693-713,723-728和332-378位的Seq ID No 196;6-21,34-44,58-64,66-74,79-87,114-127,129-143,154-162,174-189,205-214,241-262,266-273,278-297,319-324,328-338,342-351,390-398,409-415,422-435,458-464,471-477,481-486,506-531,534-540,542-550和315-389位的Seq ID No 197;4-28,39-45,52-58,69-82,93-115,122-128,135-140,146-163,177-192,209-215,221-232,271-284,331-337,341-352,360-378,383-390,392-401,409-422,428-435,462-470,474-480,482-496,531-539,541-549,551-560,562-569,576-582,598-618和98-127位的Seq ID No 198;14-27,33-47,61-79,94-104,119-133和36-60位的Seq ID No 199;11-22,29-40,48-62,68-73,96-106,108-118,125-149和102-126位的Seq ID No 200;4-11,45-55,76-83,86-102,105-112,138-144,147-153和20-48位的Seq ID No 201;12-20,28-56,62-68,72-82,93-99,101-107,120-133,135-145,178-186,208-232,279-292和36-64位的Seq ID No 202;6-14,23-48,65-82,92-134,140-181,188-219,228-238,244-253,255-261和124-145位的Seq ID No203;11-25,31-38,53-59,62-71,89-99,125-133,151-157,182-190,195-203,208-215,219-229,249-262,267-275,287-295,298-316,318-325,328-334,344-353,357-363,371-377,385-391,396-415,425-436,438-457,471-485,538-552,554-561,606-625,630-636,646-653,669-679,695-704,706-715,722-747,763-773和714-738位的Seq ID No 204;10-29,33-45,50-60,70-79,83-95,118-124,136-157,176-184,192-205,207-216,223-234,240-246,258-268,275-283和37-56位的Seq ID No 205;4-24,27-38,46-54,66-72,81-97,112-119,128-137,152-157,173-179,185-214,219-225,227-248,262-284,286-295,301-307和117-134位的Seq ID No 206;26-43,49-56,60-71,74-82,87-98,110-116,131-146,154-164,169-178,183-189,205-214,241-246,255-268,275-292,305-314,316-323,326-340,346-363,397-402,419-429,440-446,452-461,467-475和29-66位的Seq ID No 207;7-16,21-39,48-58,61-78,82-89,109-136,138-150,152-176,182-247,255-261,267-332,336-345,347-358,362-368,371-392,394-404,407-472,490-498,505-513,527-544,554-582,603-611,614-620,632-638和500-523位的Seq ID No 208;24-46,77-83,90-97,99-118,123-166,168-177,204-212,229-239,248-262,273-282,287-293,300-319,321-337,340-352,357-366,391-402,411-428,442-450,464-471,479-489和19-40位的Seq ID No 209;9-23,25-34,53-58,70-86,90-97,99-116,118-128,131-141,185-191,228-233,237-253,255-261,264-271,273-280,302-312,319-349,351-359,362-369,376-383,387-394,398-406,419-434和20-31位的Seq ID No 210;15-22,37-43,71-87,105-115,121-127,135-142,152-158和32-52位的Seq ID No 211;6-12,18-29,37-47,50-58,65-83,85-91,94-99,108-123,142-150,156-163,183-193,215-222,242-249,252-258,261-270,285-308,318-326和1-95位的Seq ID No 212;9-61,65-133,144-155,166-173,175-221,233-276,278-313,329-368和210-233位的Seq ID No 213;11-29,33-39,46-51,65-93,107-113,134-143,147-154,166-177,181-188,214-220,233-243,263-269和112-128位的Seq ID No 214;8-46,110-134,155-167,174-183,188-201,210-230,253-258,267-282,289-299,312-319,322-327,330-337,365-381,389-402,405-411,419-425,439-447,465-472,489-512,525-532,540-554,577-589,591-599,605-614,616-624,633-649和503-529位的Seq ID No 215;34-49,64-70,90-118,124-131,141-152,159-165和112-128位的Seq ID No 216;5-15,26-45,55-72,80-85,93-100,121-133,142-148,154-167,198-205,209-215,241-254,260-265,271-279和244-270位的Seq ID No 217;4-36,38-54,67-83,122-153,159-178,205-212,232-242,244-253,259-268,281-288,298-309,324-331,334-370,372-381,389-401,403-429,441-450,456-462,465-471,473-479,483-504,508-518,537-543,553-565,578-584,592-609,619-625,658-667,669-679,712-719,722-729,737-744,746-752,758-765和180-226位的Seq ID No 218;6-17,23-32,49-56,61-67,76-83,85-103,105-111,120-132,145-171,175-185,191-225,231-246和99-128位的Seq ID No 219;4-24,28-48,52-58,64-79,87-100,104-120,136-152,159-166和150-163位的Seq ID No 220;15-27,65-71,77-99,104-121,128-154,183-216,223-229,234-255,277-287,296-308和77-97位的Seq ID No 221;8-18,44-76,102-109和49-57位的Seq ID No 222;5-14,28-40,42-51,54-60,77-83,89-100,117-124,146-172,176-204,216-231,237-244,267-278,324-334,342-348,396-401,427-433,438-450,452-457,465-471,473-481,491-500,509-515,523-544,550-556,558-569,589-595,606-618,625-632,640-649,665-671,678-688,691-698,717-723,728-734,781-789,800-805,812-821,833-868,873-879,889-905,929-939,988-998,1046-1061,1073-1079,1089-1096,1115-1124,1132-1140,1172-1196,1220-1226,1231-1249,1269-1277,1287-1301,1307-1330,1350-1361,1369-1378,1387-1412,1414-1420,1422-1439,1484-1491,1513-1529,1552-1561,1576-1583,1606-1613,1617-1640,1647-1654,1665-1679,1686-1698,1709-1727,1736-1743,1750-1757,1771-1790,1801-1807,1817-1823,1831-1842,1859-1868,1870-1882,1884-1891,1900-1906,1909-1914,1929-1935,1952-1960,1974-1988,2002-2011,2032-2063,2071-2081,2116-2124,2139-2147,2149-2159,2163-2190,2209-2215,2245-2253,2282-2287,2331-2342,2360-2370,2379-2393,2402-2408,2414-2421,2423-2430,2433-2439,2442-2450,2472-2478,2485-2493,2495-2503,2506-2512,2547-2554,2558-2564,2615-2625,2637-2652,2692-2698,2700-2706,2711-2723,2731-2740,2748-2753,2756-2762,2765-2772,2781-2798,2810-2824,2844-2852,2885-2899,2912-2922,2937-2944,2947-2970,2988-2998,3016-3025,3032-3037,3062-3071,3129-3148,3156-3161和530-607位的Seq ID No 223;31-36,57-62,79-85,90-96,99-112,120-146,162-185,193-203,208-217,219-226,239-253,283-290,298-304,306-321,340-349,351-361,365-372,386-395,407-438,473-486,537-551,558-568,576-594,598-604和75-95位的Seq ID No 224;14-19,24-30,34-42,45-52,54-64,66-82,95-105,107-118,126-163,171-177,184-201,210-215,260-269,273-279,288-304,321-327,358-364,370-375,380-387,394-404,407-413,421-431,436-451,465-474,504-511,531-552,578-587,614-626,629-636,638-671,691-715,719-729,733-745,752-759,768-777,785-792,794-802,805-824,844-854,867-880,885-891,893-902,907-924,939-948,955-964,966-975,987-1000,1012-1017,1023-1028,1050-1071,1083-1098,1102-1115,1133-1146,1170-1183,1204-1211,1213-1223,1262-1311,1313-1319,1346-1355,1366-1371,1383-1405,1409-1414和776-819位的Seq ID No 225;12-27,30-38,54-61,64-74,82-96,103-110,117-125,134-140,147-158,185-201,218-225,232-253,265-280,319-325,350-362,366-372,376-386,464-483,485-490,511-521,531-537,542-559,564-574,593-609,613-619,637-642,668-677和195-214位的Seq ID No 226;4-21,59-67,73-79,84-91,141-151,186-197,203-214,222-227,237-245,255-260,281-292,294-311,336-344,346-355,422-437,459-466,484-491和77-109位的SeqID No 227;10-45,52-61,63-70,74-102,112-122,124-132,164-178,181-205,212-240,246-256和226-247位的Seq ID No 228;38-50,53-63,78-87,89-111,126-152,169-176,179-186,193-228,254-267,275-282,288-304,309-318,325-341,346-353,358-367,384-395,404-427,429-435,456-465,467-501,510-521,523-536,541-548,552-560,563-584,589-595,597-620,625-637,639-645,661-666,712-729,734-741,743-750,775-806,809-816,818-840,842-850和693-714位的Seq ID No 229;5-17,30-37,52-75,77-86,88-107,112-135,151-160,178-222,226-246,263-270,279-294,306-314,327-342,345-352,374-381,389-416,422-429,435-449,453-467,473-500,512-522,524-531,542-549,552-560,565-571,575-586,594-600,613-619,625-633,635-641,647-653,667-674,680-699,711-729,735-741,764-775,781-786,792-798,805-813,817-825,833-842,850-855,860-866,869-910,917-930,949-990和533-562位的Seq ID No 230;7-14,39-46,61-74,83-89,93-99,110-121,136-150,172-180,182-200,207-216,223-236,238-251,265-271,280-288,294-309,320-336,339-354,362-377,383-389,401-407,435-441,446-453,460-465,472-487,499-511,518-528,533-540,557-570,572-587,631-637,643-658,663-669,672-678,681-687,695-706,714-728和118-139位的Seq ID No231;5-19,24-30,56-64,69-79,93-100,102-111,117-123,125-133,174-182,185-199,205-224,268-275,311-336和102-125位的Seq ID No232;6-35,39-45,57-62,80-85,92-106,117-122,126-171,214-223,253-260,268-273,285-291,295-306,315-320,325-336,361-366和172-202位的Seq ID No 233;4-13,24-37,45-51,58-66,84-92,112-121,132-141,151-171,175-195,204-212,222-240,262-268,276-295,305-336,338-348,354-362和160-183位的Seq ID No 234;10-16,24-35,41-73,78-104,111-121,124-139,141-148,150-164,196-215,224-241,249-282,299-307,315-357,368-378,393-401和345-367位的Seq ID No235;4-32,48-53,61-67,84-104,112-118和106-130位的Seq ID No 236;21-28,31-36,65-81,98-105,115-121,123-131,136-142,155-161,177-190和201-232位的Seq ID No 237;4-15,21-27,33-39,42-56,58-64,68-82,84-90,92-98,113-122,146-162,168-175,177-189,191-203,249-268,279-285,287-304,328-342,349-358,371-378,387-393,404-413,419-425,467-479,487-498,513-524,528-539,541-565,572-579,595-606,626-635,637-642和612-626位的Seq ID No 238;7-13,52-70,76-82,97-106,110-117和13-45位的Seq ID No 239;5-10,12-48,59-64,87-102,107-128,131-140,154-161,165-171,173-215和54-74位的Seq ID No 240;4-11,19-28,34-40,74-81,87-98,126-147,163-171,184-193,205-213和49-124位的Seq ID No 241;7-14,23-29,35-40,61-67,99-106,111-122,124-133,135-161,187-206,216-229,236-245,262-268,271-280和256-273位的Seq ID No 242;4-13,17-37,47-54,85-99,105-113,120-132,147-166,180-186,192-199,204-216和127-144位的Seq ID No 243;14-27,29-37,52-62,68-76,89-96,117-123,125-131,137-145,166-195,205-212,214-222,228-235,258-264,271-281,288-296,308-324,332-339,355-361,365-371和268-293of Seq ID No244;4-21,30-42,54-60,78-85,90-110,141-147,160-168,176-185,194-206,218-225,230-245,251-261,287-293,295-304,320-326,334-347,351-362,386-402,413-423,427-433,439-453,456-477,480-493,507-513,526-539,574-581,591-598,600-609,614-632,655-665,685-691,703-712,742-747,757-775,797-803,813-819,823-829,880-887,901-906,930-944,948-958,962-968,971-995,1002-1009,1017-1023,1036-1053,1069-1081,1107-1124,1129-1152,1178-1195,1211-1223,1249-1266,1271-1288,1334-1340,1346-1367,1-63和171-189位的Seq ID No 245;4-22,52-63,70-75,94-104,112-125,133-141,176-199,209-216,244-259,287-299,336-352,366-372,386-399,421-436,444-449,457-466,481-487,506-529,531-540和295-378位的Seq ID No 246;9-30,43-49,58-75,86-96,119-131,138-147,162-167,181-201,208-214和16-121位的Seq ID No 247;4-27,52-58,80-90,92-100,108-114,118-143,169-176,189-198,247-261,281-287,307-317,323-329,352-363,372-381,396-411,413-426,429-440,442-450,456-461,468-479和1-73位的Seq ID No 248;4-32,47-52,57-63,71-78,92-104,126-142,153-175和145-163位的Seq ID No 249;17-23,35-41,51-70,73-86,104-125和105-129位的Seq ID No 250;25-32,41-50,75-85,87-103,115-122,138-149,164-171,188-210,212-220,224-234,256-273,288-299,304-310,330-336,357-365,382-390,399-405,414-421,440-446,454-461,480-486,502-514,518-540,543-553,561-567,572-580,582-588,595-630,633-651,672-681,691-709,760-767,813-832,841-848,852-866,873-893,919-925,927-933,940-955,957-978,984-997,1000-1010,1035-1040,1044-1051,1058-1064,1081-1091,1097-1124,1129-1138,1144-1150,1158-1165,1170-1180,909-936和1001-1031位的Seq ID No 251;4-12,19-26,31-41,49-64,66-86,101-117,119-127,134-142,152-161,163-172,179-188,209-218,234-241,276-291,294-300,307-320,324-341,346-356,373-387,389-397,410-416,418-436,444-454,460-472,481-486,500-507,511-535,541-549,553-559,579-586,602-607,613-620,628-640,654-663,671-678,681-691,709-722,741-754,766-774,778-786,797-803和212-226位的Seq ID No 252;4-10,15-27,34-54,60-73,79-88,101-115,120-136,154-162,167-172,222-240和126-195位的Seq ID No 253;5-16,18-25,29-35,57-63,86-91,107-121,123-131,170-179,185-199,204-226,250-255,262-274,291-296,325-347和1-38位的Seq ID No 254;7-19,22-34,36-42,48-54,60-66,71-76,104-110,118-133,135-145,158-164,167-174,182-193,196-204,217-229,251-290,293-299,309-315和288-318位的Seq ID No 255;43-51,55-61,66-73,80-90,103-127,133-142,174-180,185-196,203-210,229-235,239-251,258-266,272-278,289-314,316-326,340-346,355-361和14-27位的Seq ID No 256;4-25,27-33,35-41,52-74,76-89,99-124,138-144,146-159,167-182,184-191,193-206,211-223,232-240,249-257,270-279,281-287,293-310,322-341,347-356和292-322位的Seq ID No257;5-13,28-38,43-60,67-72,98-116,122-134,137-151,167-174,177-195,197-216和99-195位的Seq ID No 258;15-33,35-42,48-57,62-68,73-91,107-119,121-153,173-194,205-210,223-228,234-241,243-259,275-298,308-315,327-340,342-370,376-391,398-404,410-419和71-122位的Seq ID No 259;12-39,43-64,87-95,99-105,114-126,128-136,139-147,212-225和107-141位的Seq ID No 260;6-33,40-45,60-75,79-86,121-129,131-137,161-167,172-178,186-195,203-212,236-244,257-264,278-294,306-312,345-358,368-381,386-395,404-410,412-418和198-270位的Seq ID No 261;18-31,34-41,50-56,69-83,99-106,129-141,147-153,159-168,170-178,190-198,200-212,221-232,237-255,261-266,274-292和118-216位的Seq ID No262;17-47,61-67,87-93,115-121,126-132,140-148,167-173,179-186,214-223,250-255,264-272,282-294,306-318,338-353,358-377,385-401,414-420,433-441,451-457,470-480,505-511,544-550,571-581,600-607,612-618,631-648,655-662,669-681,693-714,721-726,733-740,757-778,813-823,831-838,851-857,866-876,893-905,912-917,930-936,951-963,971-981,1008-1019,1021-1033,1035-1041,1054-1064,1066-1076,1097-1110,1113-1121,1126-1140,1159-1171,1182-1195,1197-1203,1216-1222,1231-1240,1243-1262,1268-1287和738-828位的Seq ID No 263;19-28,40-46,51-57,68-74,81-87,98-108,111-121和20-36位的Seq ID No 264;4-17,19-44,60-69,80-86,110-116和33-60位的Seq ID No 265;8-16,18-28,42-50,53-75,79-86,94-99,122-128,136-142,149-163,166-173,198-212,254-272,288-295,304-318,324-329,343-348,351-364,367-383,389-395,411-420,427-436和11-56位的Seq ID No 266;19-25和6-24位的Seq IDNo 267;6-39,59-68和44-63位的Seq ID No 268;5-14,21-28,38-53和29-41位的Seq ID No 269;4-13,31-41,56-65和32-56位的Seq ID No 270;5-12和4-21位的Seq ID No 271;4-18和17-32位的Seq ID No 272;4-10,23-33和14-30位的Seq ID No 273;26-34,44-53和35-52位的Seq ID No274;1-19位的Seq ID No 275;4-17,23-30,32-37和6-23位的Seq ID No276;5-33,40-58,61-66和45-66位的Seq ID No 277;15-41,61-67和41-65位的Seq ID No 278;4-12,16-23,26-37和10-29位的Seq ID No 279;23-39和37-55位的Seq ID No 280;12-20和38-55位的Seq ID No 281;22-37和7-22位的Seq ID No 282;3-14位的Seq ID No 283;6-16,43-65,71-76和17-31位的Seq ID No 284;4-13,27-39,42-69和17-32位的SeqID No 285;4-12,26-39和10-25位的Seq ID No 286;2-31位的Seq ID No287;6-38,49-62和39-55位的Seq ID No 288;4-10,24-30和2-19位的Seq ID No 289;12-17,25-46和15-30位的Seq ID No 290;4-13和2-28位的Seq ID No 291;30-38和17-45位的Seq ID No 292;24-33,55-61和31-61位的Seq ID No 293;4-26,34-48和15-33位的Seq ID No 294;9-15和1-22位的Seq ID No 295;4-31和14-33位的Seq ID No 296;5-34,49-55,64-82和69-83位的Seq ID No 297;33-45和21-39位的Seq ID No298;7-14,24-32,42-65,79-86和50-64位的Seq ID No 299;13-27,33-43,45-62和12-37位的Seq ID No 300;4-15,17-32和10-26位的Seq ID No301;4-9,11-43,45-75和47-69位的Seq ID No 302;4-18,22-37和17-34位的Seq ID No 303;4-14和5-24位的Seq ID No 304;7-33,35-46和1-19位的Seq ID No 305;13-37,69-75和51-69位的Seq ID No 306;14-24,26-34,37-49,66-78和2-25位的Seq ID No 307;17-46,52-57,59-64和54-68位的Seq ID No 308;4-22和13-25位的Seq ID No 309;8-40,53-63和29-50位的Seq ID No 310;16-28和32-40位的Seq ID No 311;14-20,22-28,39-45和2-22位的Seq ID No 312;4-13和12-31位的Seq ID No 313;15-21和2-17位的Seq ID No 314;4-17和20-36位的Seq ID No 315;4-19和9-18位的Seq ID No 316;4-14和3-19位的Seq ID No 317;4-21,32-40和21-39位的Seq ID No 318;4-13和10-27位的Seq ID No 319;18-31,39-47,75-87,89-98和79-99位的Seq ID No 320;15-21和9-24位的SeqID No 321;4-14,18-27,30-53,55-64,68-74,81-98和22-40位的Seq IDNo 322;7-24,44-51和35-60位的Seq ID No 323;10-47和23-37位的SeqID No 324;4-10,12-46和7-22位的Seq ID No 325;20-27和1-13位的SeqID No 326;6-19,41-51和9-37位的Seq ID No 327;4-9,11-17和9-23位的Seq ID No 328;4-17,23-38,46-66,68-85和34-46位的Seq ID No 329;4-18,34-59,75-81和61-84位的Seq ID No 330;6-17和7-28位的Seq IDNo 331;4-32,56-61和35-52位的Seq ID No 332;4-14,27-71,74-88,93-110,115-120,124-130,139-154,161-172和146-171位的Seq ID No333;4-21和3-15位的Seq ID No 334;12-17和9-26位的Seq ID No 335;10-21,45-58和51-67位的Seq ID No 336;59-66,68-84和13-42位的SeqID No 337;11-16和1-16位的Seq ID No 338;4-19,23-37和10-30位的Seq ID No 339;19-27,35-46,48-66,82-88,99-105,113-119和42-59位的Seq ID No 340;135-147位的Seq ID No 171;658-682位的Seq ID No172;411-427和1226-1246位的Seq ID No 173;794-817和801-824位的Seq ID No 174;468-492和474-495位的Seq ID No 177;366-388位的SeqID No 178;266-291,287-312和308-333位的Seq ID No 179;197-213和195-211位的Seq ID No 180;252-275,262-285和812-830位的Seq ID No181;94-112,97-120和104-128位的Seq ID No 182;257-281位的Seq IDNo 183;106-134位的Seq ID No 184;70-86位的Seq ID No 187;358-383,378-402,397-421,499-524,520-545,541-566,622-646,641-665和660-684位的Seq ID No 189;248-260位的Seq ID No 192;15-34位的SeqID No 194;112-129位的Seq ID No 195;333-358和353-378位的Seq IDNo 196;316-343,339-366和362-389位的Seq ID No 197;98-123和104-126位的Seq ID No 198;20-43和23-48位的Seq ID No 201;124-145位的Seq ID No 203;717-738位的Seq ID No 204;37-56位的Seq ID No205;118-134位的Seq ID No 206;500-522位的Seq ID No 208;32-47位的Seq ID No 211;25-51,47-73和69-95位的Seq ID No 212;503-529位的Seq ID No 215;112-128位的Seq ID No 216;181-199位的Seq ID No218;109-121位的Seq ID No 219;150-163位的Seq ID No 220;77-97位的Seq ID No 221;564-586位的Seq ID No 223;75-94位的Seq ID No224;776-798,784-808和794-815位的Seq ID No 225;196-212,78-100和85-107位的Seq ID No 226;536-553位的Seq ID No 230;102-125位的Seq ID No 232;178-198位的Seq ID No 233;612-626位的Seq ID No238;171-187位的Seq ID No 245;296-320,315-339,334-358和353-377位的Seq ID No 246;47-71位的Seq ID No 247;1-25,20-45和40-64位的Seq ID No 248;146-161位的Seq ID No 249;910-935和1007-1030位的Seq ID No 251;212-226位的Seq ID No 252;126-152,148-173和169-195位的Seq ID No 253;288-310和293-316位的Seq ID No 255;293-312位的Seq ID No 257;154-170位的Seq ID No 258;72-95,90-112和97-121位的Seq ID No 259;135-150和146-163位的Seq ID No 262;799-827位的Seq ID No 263;23-43和33-53位的Seq ID No 266;44-62位的Seq ID No 268;6-22位的Seq ID No 276;37-54位的Seq ID No 280;40-54位的Seq ID No 281;7-21位的Seq ID No 282;4-11,16-34,48-55,67-77,87-106和153-183位的Seq ID No 425;22-40,49-65,70-91,95-109,111-125,146-207,209-216,219-225,229-244,251-270,274-286,292-309,316-329,335-355,358-370,376-388,392-419,425-430,435-441,448-455,464-478,486-515和437-465位的Seq 1D No 426;5-19,25-31,43-48,60-79,88-100,105-129,148-171,187-193,243-263,316-322,334-340,345-351,369-378,381-391,399-404,474-483,502-517,525-530,558-568,579-596,622-627,631-638,644-651,653-660,674-680,687-693,721-728,743-753,760-775,788-795,806-813,821-828,835-842,847-859,868-887和300-347位的Seq ID No427;5-26,37-44,89-97,112-118,121-128,138-154,157-165,176-181,188-198,205-218,223-243,247-253,260-279和76-155位的Seq ID No428;4-29,41-46,49-68,82-88,121-147,158-164,187-193,195-208,229-236,244-249,251-263,269-275,307-313,337-343,348-381,392-398,402-408,432-438,85-117和194-239位的Seq ID No 429;5-12,14-22,28-34,40-46,70-79,84-129,152-165,174-182和37-109位的SeqID No 430;5-16,18-52,54-72,81-86,118-126,136-145,151-157,168-180,209-233,244-270,295-302,315-326,329-337,345-352,364-373,397-402,408-418,424-431,435-443,472-480,483-489,504-510,519-527,549-564,576-599,605-637,641-673和91-98位的SeqID No 431;23-36,42-52,133-140,151-157,242-247,267-277,295-301,320-328,333-339,345-352,365-371,397-403,415-428,456-465,481-487,489-495,508-516,518-527,585-592,606-614,631-637,643-658,665-670,723-728,737-744,752-759,787-793,835-841,873-885,918-928,938-945,951-966,978-988,1015-1020,1030-1036,1044-1052,1058-1069,1071-1079,1081-1088,1113-1119,1125-1138,1141-1147,1164-1170,1172-1177,1190-1200,1214-1220,1230-1236,1239-1245,1262-1268,1270-1275,1288-1298,1312-1318,1328-1334,1337-1343,1360-1366,1368-1373,1386-1396,1410-1416,1426-1432,1435-1441,1458-1464,1466-1471,1484-1494,1508-1514,1524-1530,1533-1539,1556-1562和307-340位的Seq ID No 432;19-25,35-41,44-50,66-72,74-79,92-102,116-122,132-138,141-147,164-170,172-177,190-200,214-220,230-236,239-245,262-268,270-275,288-298,312-318,328-334,337-343,360-366,368-373,386-396,410-416,426-432,435-441,458-464,466-478,504-524,79-148,177-246,275-344和373-442位的Seq ID No 433;7-14,16-23,33-39,46-53,72-79,92-115,123-130,156-175,179-187,214-220,239-246,266-274,302-325,338-354,360-370,375-390,392-401,421-428,430-463和29-58位的SeqID No 434;4-9,22-39,58-65,72-82,87-92,99-104,107-119,143-166,171-177,194-202,205-213,220-228,231-240,247-263,309-315,317-323,336-343和294-320位的Seq ID No 435;4-10,12-18,24-29,34-43,50-65,70-76,111-117,129-138,152-159,166-171,184-195,200-210,224-236,241-251,274-283,285-296,313-319,332-341,348-355,378-386,410-416,433-445,475-482,523-529,531-540,584-596,626-633,674-680,682-688,738-750,780-787,828-834,836-842,853-862,882-887,893-912和604-676位的Seq ID No 436;15-38,49-57,60-99,103-119,124-194,200-206,215-249,251-291,307-313,315-347,369-378,383-390,393-400,405-411,423-435,440-447,454-460,470-486,490-503,532-539,542-549,551-567,579-592和509-583位的Seq ID No 437;38-44,47-88,95-103,157-172,235-240,250-260,263-276,294-300,312-317,331-337,369-391,412-419,442-448,453-463,490-529,537-555,571-580,600-617,619-627,642-648,682-687,693-700,716-722,738-748,756-763,779-789,796-802,820-828,833-840,846-853,862-872,880-887,894-899,924-937,957-963,1006-1012,1043-1049,1063-1069,1076-1097和124-147位的Seq ID No 438;4-28,31-49,60-71,75-102,104-114,134-144,160-184,250-257,277-285,287-294,330-338,345-351,367-374,381-388,393-399,402-407,420-426,443-448,458-464,411-436和454-488位的Seq ID No 439;20-27,45-55,57-64,66-77,98-106,130-137,155-165,167-174,176-187,194-203,208-223,227-238,245-251,257-270,273-278,287-299,330-345,352-358,363-385,392-399,410-417,437-443,467-484,486-492,495-500,504-516,526-536和219-270位的Seq ID No 440;11-22,24-31,46-63,65-71,73-88,95-109,174-181,183-201,204-212,216-222,228-233,241-247和142-221位的Seq ID No 441;8-28,51-59,67-84,93-98,140-152,154-162,183-188和91-125位的Seq ID No 442;10-22,27-61和69-100位的Seq ID No 443;7-15,18-26,94-100,126-131,152-165,219-228,254-263,274-292,297-308,333-340,342-352,354-371,373-379,403-410,420-438,450-456,463-470,489-495,503-512和97-173位的Seq ID No 444;4-21,37-43,49-65,67-74,76-90,113-119,131-141,155-173,175-189,192-199,207-221,247-254,266-276,317-322,337-343,387-393,408-428,439-448,451-460,469-479,482-487,493-501,517-523,533-542和480-503位的Seq ID No 445;11-26,40-46,78-86,93-103,121-126,132-138,166-177,183-196,203-212,214-221,228-263,304-311,323-338,345-351,357-363,379-393,420-434,442-448,518-527,547-553,581-591,602-609,637-645,665-674,687-692,701-708,730-739,796-802,844-857,882-888,903-914,944-950,976-983,1027-1033,1049-1057,1066-1072,1085-1092,1120-1127,1137-1144,1153-1158,1165-1176,1181-1187,1221-1230,1238-1244,1269-1274和605-632位的Seq ID No 446;6-47,57-65,83-95,109-121,138-147,154-164,167-177,194-200,202-212,227-234,240-253,260-267,283-291,320-329,340-347,356-364,412-422,430-436,441-459,465-475,478-486,498-507和59-84位的SeqID No 447;10-21,58-83,88-97,120-126和21-51位的Seq ID No 448;5-39,56-62,76-88,90-114,138-162,170-195,202-221,228-250,264-270,304-355,374-387,391-416,462-471,526-546,554-561,574-579,639-645,651-660,674-682,689-694和666-697位的Seq ID No 449;6-30,36-42,143-157,176-197,202-209,216-233,241-246,275-287,292-299,315-325,343-350,375-380,397-403,411-420,422-434,441-448,467-474,477-499,555-568,591-597,601-609,623-644,667-688,692-698,703-718,736-747,757-766,782-791,795-801,832-840,859-865和226-269位的Seq ID No 450;6-23,43-51,61-67,73-82,91-97,123-130,149-158,164-175,228-234,240-246,248-255,262-272,326-332,340-347,365-371,377-388,409-419,425-431,438-445,449-457,464-470,496-507,559-568,575-581,603-608,617-623,633-639,648-654,659-670,695-701,734-752,806-814,816-829,861-868,891-899,904-909,937-945,947-960,978-983,992-999,1022-1031,1068-1076,1078-1091,1109-1114,1123-1130,1153-1162,1199-1207,1209-1222,1254-1261,1284-1293,1330-1338,1340-1353,1371-1376,1385-1392,1415-1421,1433-1438,1460-1465,1470-1492和1422-1458位的Seq ID No 451;82-94,111-118,125-131,206-212,261-266,310-320,328-338,345-351,353-360,414-420,424-434,440-447,451-500,506-516,548-561,566-572,584-591,601-622,630-636,650-659,661-667,674-699,703-711,717-729,736-744,752-759,765-771,813-822,826-842,852-868,870-877,881-895,897-906,913-922和602-671位的Seq ID No 452;12-18,20-25,43-54,56-65,73-79,82-88,99-111,136-142,153-169,171-183,195-223,229-248,255-260,272-277,281-292,298-319,322-329,332-351,363-379,381-389和275-304位的Seq ID No 453;4-9,34-48,65-77,101-106,111-131,138-153,186-191,230-250和148-219位的Seq ID No454;4-23,30-35,42-53,67-76,82-87,101-108,112-130,132-138,147-152,161-183,187-208,218-225,265-283,295-303,306-317,322-334,338-357,360-368,370-383,387-398,400-419,421-430,104-182和240-304位的Seq ID No 455;4-12,63-69,94-102,146-164,166-173,175-181,193-207,263-281,286-295,301-306,330-343,369-378,382-388,414-420,422-430,438-454,456-462,472-531,543-560,581-591,596-605,614-623,626-635,656-662,669-676,683-690,693-698,705-711,728-736,752-764和69-102位的Seq ID No456;6-12,43-53,141-147,164-179,185-195,197-206,227-235,237-271,288-305,308-317,335-341,351-357,365-376,386-395,397-416,422-447和11-35位的Seq ID No 457;16-24,50-65,73-84,88-99,114-124,130-146,181-187,193-203,214-220,236-247,250-258,287-297和50-113位的Seq ID No 458;4-25,50-55,76-82,117-123,131-137,139-148,157-166,239-245,253-258,266-275,277-292,300-306,51-83和93-161位的Seq ID No 459;6-22,34-43,51-86,93-100,110-116,150-161,164-171,180-187,197-218和168-237位的Seq ID No 460;4-27,55-60,74-82和10-46位的Seq ID No 461;6-19,25-31,43-49,60-79,88-100,105-129,148-161,164-171,187-193,243-263,316-322,334-340,369-378,381-391,398-404,460-466,474-483,502-509,511-517,525-530,558-567,579-596,622-627,631-638,641-651,653-659,674-680,687-693,710-716,720-727,743-753,760-775,788-795,806-813,821-828,836-842,847-860,865-880和258-377位的Seq ID No462;4-11,25-64,71-79,88-94,107-120,123-132,167-188,231-237,240-246,261-267,306-311,330-342,351-358,389-395,406-418,429-434,439-448,483-501,511-520和71-143位的Seq ID No 463;4-18,22-27,53-64,94-100,121-127,133-139,155-164,177-182,187-196,206-218,224-242,248-253,258-277和184-253位的Seq ID No 464;10-17,56-67,72-82,94-99,106-113,166-173,229-235,243-283,295-301,313-321,326-331,342-348,396-414,423-435,446-452,454-462,496-502,511-534,543-556,563-570,586-593,616-626,638-645,653-662,679-696,731-737,766-774,776-782,790-796,810-817,825-835,837-846和540-615位的Seq ID No 465;13-24,30-36,73-81,89-95,109-115,117-143,161-173,179-189,226-244,251-261,275-281,298-305,307-315,323-328,364-374,69-186和264-354位的Seq ID No466;19-25和6-22位的Seq ID No 467;6-39,59-68和43-62位的Seq IDNo 468;6-14,22-32和1-27位的Seq ID No 469;4-41和28-40位的Seq IDNo 470;8-14和4-19位的Seq ID No 471;4-10,12-22,30-35和6-33位的Seq ID No 472;4-16,24-33和37-54位的Seq ID No 473;2-23位的SeqID No 474;4-21,27-33,36-41和14-34位的Seq ID No 475;4-14,24-30,37-42,57-78,83-89,94-103,113-131和100-122位的Seq ID No 476。
本发明也提供了生产根据本发明的粪肠球菌超免疫血清反应性抗原或其片段的方法,包括在合适的表达系统中表达根据本发明的一个或多个核酸分子。
此外,本发明提供了生产表达根据本发明的粪肠球菌超免疫血清反应性抗原或其片段的细胞的方法,包括用根据本发明的载体转化或转染合适的宿主细胞。
根据本发明提供了尤其是疫苗的药物组合物,其包含如本发明定义的超免疫血清反应性抗原或其片段或如本发明定义的核酸分子。
优选实施方案中,药物组合物进一步包含了免疫刺激物质,优选地选自尤其是聚阳离子肽的聚阳离子聚合物、免疫刺激脱氧核苷酸(ODN)、含有尤其是KLKL5KLK的至少两个LysLeuLys基团的肽、尤其是人生长激素的神经活性化合物、明矾(alumn)、弗氏完全或不完全佐剂或它们的组合。
更加优选的实施方案中,免疫刺激物质是聚阳离子聚合物和免疫刺激脱氧核苷酸或含有至少两个LysLeuLys基团的肽和免疫刺激脱氧核苷酸的组合。
还更优选的实施方案中,聚阳离子聚合物是聚阳离子肽,尤其是聚精氨酸。
根据本发明,提供了根据本发明的核酸或根据本发明的超免疫血清反应性抗原或其片段用于药物制备物的生产的用途,尤其是用于抗肠球菌感染的疫苗的生产。
同此,也提供了至少结合于根据本发明的超免疫血清反应性抗原或其片段的选择性部分的抗体或其至少有效部分。
优选的实施方案中抗体是单克隆抗体。
另一优选的实施方案中抗体的有效部分包含Fab片段。
进一步优选的实施方案中抗体是嵌合抗体。
还优选的实施方案中抗体是人源化抗体。
本发明也提供了产生根据本发明的抗体的杂交瘤细胞系。
此外,本发明提供了产生根据本发明的抗体的方法,特征在于如下的步骤:
·通过给予非人的动物如本发明中定义的超免疫血清反应性抗原或其片段引发所述动物的免疫反应,
·从所述动物移出含抗体的体液,以及
·通过进一步纯化步骤处理含所述抗体的体液产生抗体。
相应地,本发明也提供了一种产生根据本发明的抗体的方法,特征在于如下的步骤:
·通过给予非人的动物如本发明中定义的超免疫血清反应性抗原或其片段引发所述动物的免疫反应,
·从所述动物移出脾或脾细胞,
·生产所述脾或脾细胞的杂交瘤细胞
·挑选和克隆特异于所述超免疫血清反应性抗原或其片段的杂交瘤细胞,
·通过所述克隆的杂交瘤细胞的培养和可选的进一步纯化步骤产生抗体。
根据上面方法提供或生产的抗体可以用于治疗或预防肠球菌感染的药剂的制备。
根据另一方面,本发明提供了结合根据本发明的超免疫血清反应性抗原或其片段的拮抗剂。
所述能够结合根据本发明的超免疫血清反应性抗原或其片段的拮抗剂可以通过包含如下步骤的方法鉴别:
a)将分离的或固定化的根据本发明的超免疫血清反应性抗原或其片段在允许候选拮抗剂和所述超免疫血清反应性抗原或其片段结合的条件下,在能够提供应答于候选拮抗剂和所述超免疫血清反应性抗原或其片段结合的可检测信号的组分的存在下,与所述候选拮抗剂接触;以及
b)检测应答于拮抗剂与超免疫血清反应性抗原或其片段结合的信号的存在或不存在。
能够降低或抑制根据本发明的超免疫血清反应性抗原或其片段与其相互反应伴侣的相互反应活性的拮抗剂可以通过包含如下步骤的方法鉴别:
a)提供了根据本发明的超免疫血清反应性抗原或其超免疫片段,
b)向所述超免疫血清反应性抗原或其片段,提供相互反应伴侣,尤其是根据本发明的抗体,
c)允许所述超免疫血清反应性抗原或其片段与所述相互反应伴侣的相互反应以形成相互反应复合物,
d)提供候选拮抗剂,
e)允许候选拮抗剂和相互反应复合物之间发生竞争反应,
f)测定候选拮抗剂是否抑制或降低超免疫血清反应性抗原或其片段与相互反应伴侣的相互反应活性。
根据本发明的超免疫血清反应性抗原或其片段可用于所述超免疫血清反应性抗原或其片段的相互反应伴侣的分离和/或纯化和/或鉴别。
本发明也提供了体外诊断与根据本发明的超免疫血清反应性抗原或其片段的表达相关的疾病的方法,包括测定根据本发明编码所述超免疫血清反应性抗原或其片段的核酸序列的存在,或根据本发明的超免疫血清反应性抗原或其片段的存在。
本发明也提供了尤其是肠球菌感染的细菌感染体外诊断的方法,包括分析根据本发明编码所述超免疫血清反应性抗原或其片段的核酸序列的存在或根据本发明的超免疫血清反应性抗原或其片段的存在。
此外,本发明提供了根据本发明的超免疫血清反应性抗原或其片段的用途其用以产生结合于所述超免疫血清反应性抗原或其片段的肽,其中所述肽是抗促成素(anticaline)。
本发明也提供了根据本发明的超免疫血清反应性抗原或其片段的用途,其用以生产功能性核酸,其中所述功能性核酸选自适体(aptamer)和镜面体(spiegelmers)。
根据本发明的核酸分子也可用于功能性核糖核酸的生产,其中所述功能性核糖核酸选自核酶、反义核酸和siRNA。
本发明有利地提供了使用来自多个人血浆合并物的抗体制备物和来源于粪肠球菌基因组的表面表达文库,从粪肠球菌鉴别出来的一套有效的、相关的和全面的分离的核酸分子和它们编码的超免疫血清反应性抗原或其片段。因而,本发明满足了在制备抗体和鉴别有效对抗肠球菌感染的化合物的过程中有用的粪肠球菌抗原、疫苗、诊断和产品的广泛需要。
有效疫苗应当由所有株表达的并能诱导抗粪肠球菌细胞表面组分的高亲和力、丰富抗体的蛋白质或多肽组成。对于调理作用,抗体应当是IgG1和/或IgG3,对于粘附和毒素作用的中和,是任何IgG亚型和IgA。化学限定的疫苗必须是明确地比全细胞疫苗(减毒或灭活的)优良,因为与人类组织交叉反应或抑制调理作用的粪肠球菌的组分能被去除(Whitnack,E.等人,1985),并且诱导保护抗体和/或保护免疫反应的个体蛋白质能被挑选出来。
本发明采用的方法是基于肠球菌蛋白质或肽与人血清中存在的抗体的相互作用。人免疫系统产生的并且在人血清中存在的抗粪肠球菌抗体是抗原蛋白质的体内表达和它们的免疫原性的指征。另外,在用个体选择或产生的血清进行的第二和第三轮筛选中处理抗原蛋白质,所述抗原蛋白质是使用预选血清的合并物通过细菌表面展示表达文库鉴别的。因而,本发明提供了作为药物组合物尤其是预防粪肠球菌感染的疫苗的一套有效、相关、全面的肠球菌抗原。
在鉴别根据本发明的一全套抗原的抗原鉴别程序中,用一些血清合并物或血浆级分或其它合并的含有抗体的体液(抗体合并物)筛选了至少两个不同的细菌表面表达文库。抗体合并物来源于已经测试了抗粪肠球菌抗原化合物的血清收集物,例如全部细胞提取物和培养上清蛋白质。优选地,使用两个截然不同的血清收集物,1.带有非常稳定的抗体储备库:正常成人,临床上健康的人群,其不是携带者且经历此前的接触或目前是粪肠球菌携带者但无急性疾病或症状,2.带有通过致病生物存在急性诱导的抗体:具有不同表现的患有急性病症的患者(如粪肠球菌心内膜炎,尿道感染和菌血症)。为了考虑是超免疫的并因而在本发明采用的筛选方法中是相关的,血清必须与多个肠球菌特异性抗原反应。通过人免疫系统针对肠球菌产生的且存在于人血清中的抗体是抗原性蛋白质体内表达及其免疫原性的指征。
如本发明中使用的表达文库应当允许所有可能抗原,例如源自粪肠球菌所有表面蛋白质的表达。细菌表面展示文库由在细菌宿主膜的所选的外膜蛋白质(LamB,BtuB和FhuA)的呈示肠球菌一(总)套表达肽序列的细菌宿主的重组文库表示{Georgiou,G.,1997};{Etz,H.等人,2001}。使用重组表达文库一个优点是鉴别的超免疫血清反应性抗原可以立即由筛选的和挑选的表达超免疫血清反应性抗原的克隆的编码序列的表达产生,没有进一步的重组DNA技术或必要的克隆步骤。
进一步用一或多个额外轮的筛选,分析通过根据本发明描述的程序鉴别的全套抗原。所以,使用了被鉴别有免疫原性的个体抗体制备物或产生的抗所选肽的抗体。根据优选实施方案,用于第二轮筛选的个体抗体制备物来源于患有肠球菌急性感染的患者,尤其是显示抗体滴度高于某些最低水平的患者,例如,抗体滴度高于80%,优选高于90%,尤其优选高于95%的所测试的人类(患者或健康个体)血清的抗体滴度。在第二轮筛选使用这样高滴度的个体抗体制备物允许超免疫血清反应性抗原和其片段从粪肠球菌的非常有选择性的鉴别。
全面筛选过程之后,通过一系列ELISA和Western斑点试验在第二轮筛选中测试了挑选的抗原蛋白质以评价它们对大量的人血清收集物(大于100未感染的和大于50个患者血清)的免疫原性,所述抗原蛋白质在原核表达系统中不能表达的情况下表达为重组蛋白质或体外翻译产物,或者鉴别的抗原性肽(合成产生)。
个体抗体制备物(其也可是挑选的血清)允许所有超免疫血清反应性抗原最有希望的候选物从第一轮的所有有希望的候选物中的选择性鉴别是重要的。所以,优选地至少10个个体抗体制备物(即来自至少10个已经遭受所选病原体感染的不同个体的抗体制备物(例如血清))应当用于在第二轮筛选中鉴别这些抗原。当然,也可使用少于10个个体制备物,然而,步骤的选择性与低数量的个体抗体制备物可能不是最佳的。另一方面,如果给定的超免疫血清反应性抗原(或其抗原片段)被至少10个个体抗体制备物,优选地至少30个,尤其是至少50个个体抗体制备物识别,那么超免疫血清反应性抗原的鉴别对正确鉴别也有足够的选择性。当然也可以与尽可能多的个体制备物(例如与多于100或甚至与多于1,000个)测试超免疫血清反应。
所以,根据本发明的方法之超免疫血清反应抗体制备物的相关部分应当优选地至少10个,更加优选地至少30个,尤其是至少50个个体抗体制备物。或者(或联合地)超免疫血清反应性抗原优选地也可用第二轮筛选中使用的至少20%,优选地至少30%,尤其是至少40%的所有个体抗体制备物进行鉴别。
根据本发明的优选实施方案,用于第二轮筛选个体抗体制备物从其制备的血清通过它们抗粪肠球菌的滴度(例如抗这个病原体的制备物,例如裂解物,细胞壁组分和重组蛋白质)进行挑选。优选地,当ELISA中全生物体(总裂解物或总细胞)用作抗原时,挑选有总IgG滴度高于10,000U尤其是高于12,000U(U=单位,从在给定稀释度的OD405nm读数计算)的一些血清。
人免疫系统产生的针对肠球菌、并存在于人血清中的抗体是抗原蛋白质的体内表达和它们的免疫原性的指征。血清抗体识别的线性表位之识别能基于如4-5个氨基酸短的序列。当然,它不是一定意味着这些短肽能够体内诱导给定的抗体。为了那个原因,定义的表位、多肽和蛋白质进一步地在动物(主要是小鼠)中测试它们诱导抗体内挑选蛋白质的抗体的能力。
优选的抗原位于细胞表面或被分泌,并且因而可胞外接近。期望抗细胞壁蛋白质的抗体起两个作用:抑制粘附、和促进噬菌作用。针对分泌蛋白质的抗体对它们作为毒素或毒力组分的功能的中和是有益的。细菌通过分泌蛋白质相互联系也是已知的。抗这些蛋白质之中和抗体将干扰肠球菌种属之间或肠球菌内的促进生长的联系(crosstalk)。生物信息分析(信号序列、细胞壁定位信号、跨膜结构域)证明对于评价细胞表面定位或分泌是非常有用的。实验方法包括有相应表位的抗体和蛋白质从人血清的分离,以及小鼠中抗细菌表面展示筛选挑选的(多)肽的免疫血清的产生。
为了所述目的,将细菌大肠杆菌菌落直接注射进小鼠,收集免疫血清并在相关体外试验中测试功能性调理或中和抗体。或者,可以使用肽或蛋白质作为底物从人或小鼠血清纯化特异性抗体。
针对粪肠球菌的宿主防御主要依赖调理噬菌性杀死机制。通过疫苗接种诱导高亲和力的调理和中和类型的抗体帮助先天免疫系统清除细菌和毒素。这个使得根据本发明的方法成为肠球菌抗原蛋白质鉴别的最佳工具。
皮肤和粘膜的膜是强大的抗肠球菌入侵的屏障。然而,一旦皮肤或粘附的膜被破坏,第一道非适应性细胞防疫通过补体和噬菌细胞尤其是多形核白细胞(PMN)开始它的合作作用。这些细胞能被当作清除入侵细菌的基石。因为粪肠球菌是主要的细胞外病原体,主要的抗肠球菌适应性反应来自免疫系统的体液途径,并由三个主要机制介导:调理作用的促进、毒素的中和和粘附的抑制。相信调理作用因为它对有效噬菌作用的要求是尤其重要的。对于有效的调理作用,为了PMN通过IgG分子Fc片段或激活的C3b的受体的识别,微生物表面必须用抗体和补体因子包被。调理作用之后,肠球菌被吞噬和杀死。结合于细菌细胞表面上的特异性抗原的抗体起附着于PMN的配体和促进噬菌作用的作用。期望结合于粘附素和其它细胞表面蛋白质的非常一样的抗体中和粘附以及阻止群集。本发明提供的抗原的选择因而非常适于鉴别将导致抗动物模型或人类中抗感染保护的那些抗原。
根据此处使用的抗原鉴别方法,本发明惊人地提供了尤其如下描述的一套全面的粪肠球菌新型核酸和新型超免疫血清反应性抗原和其片段。根据一个方面,本发明尤其涉及编码超免疫血清反应性抗原的核苷酸序列,所述序列列于序列列表中Seq ID No 1-170,373-424,以及代表超免疫血清反应性抗原的相应的编码氨基酸序列列于序列列表中Seq ID No 171-340和425-476。
本发明优选实施方案中,提供了其全长展示了与Seq ID No 1-2,4-8,10,12-18,20-23,25-26,29-43,45-62,64-74,76-77,79-83,85-89,91-92,94-114,117-126,128-146,148-170,373,375,379-381,387,392,394,397-399,407-408,410-411和415-424列出的核苷酸序列70%相同性的核酸分子。最优选的是包含其全长与Seq ID No 1-2,4-8,10,12-18,20-23,25-26,29-43,45-62,64-74,76-77,79-83,85-89,91-92,94-114,117-126,128-146,148-170,373,375,379-381,387,392,394,397-399,407-408,410-411和415-424列出的核酸分子至少80%或至少85%相同性的区域之核酸。在这点上,核酸分子就其全长与上述核酸分子至少90%、91%、92%、93%、94%、95%或96%相同是尤其优选的。此外,有至少97%相同性的那些是优选的,有至少98%和至少99%的那些是尤其高度优选的,有至少99%或99.5%是更加优选的,有100%相同性是尤其优选的。另外,这点上优选实施方案是编码基本上保留了与Seq ID No 1-2,4-8,10,12-18,20-23,25-26,29-43,45-62,64-74,76-77,79-83,85-89,91-92,94-114,117-126,128-146,148-170,373,375,379-381,387,392,394,397-399,407-408,410-411和415-424列出的所述核酸编码的成熟多肽相同生物功能或活性的超免疫血清反应性抗原或其片段的核酸。
如本领域已知并此处使用的相同性是通过比较序列测定的两个或两个以上多肽序列或两个或两个以上多核苷酸序列之间的关系。本领域中,相同性也指多肽或聚核苷酸序列之间序列相关的程度,就如在本情况下可以是这样的序列串(strings)之间的匹配测定的。能很容易地计算相同性。虽然有许多方法测量两个聚核苷酸或两个多肽序列之间的相同性,所述术语对于技术人员是众所周知的(例如分子生物学中的序列分析,von Heinje,G.,Academic Press,1987)。设计测定相同性的优选方法以给予测试的序列之间最大的匹配。在计算机程序中编码测定相同性的方法。测定两个序列之间相同性的优选计算机程序方法包括但不限于GCG程序包{Devereux,J.等人,1984}、BLASTP、BLASTN和FASTA{Altschul,S.等人,1990}。
根据本发明的另一方面,提供了展现与Seq ID No 3,9,11,24,27,44,63,75,84,115-116,127,374,376-378,382-386,388-391,393,395-396,400-406,409和412-414列出的核酸序列有96%或高于96%,尤其是100%序列相同性的核酸分子。
根据本发明的另一方面,提供了与核酸分子具有98%或高于98%,尤其是100%序列相同性的核酸分子,所述核酸分子与Seq ID No 90,147列出的核酸序列相同。
根据本发明的核酸分子作为第二备择方案也可是至少基本上互补于上述第一备择方案的核酸的核酸分子。如此处使用地,互补是指核酸链经由Watson-Crick碱基配对与第二个核酸链是碱基配对的。如此处使用地基本上互补的是指碱基配对不发生在各个链所有的碱基而留有一定数量或百分比的未配对或错配的碱基。正确配对碱基的百分比优选地是至少70%,更加优选地80%,甚至更加优选地90%以及最优选地高于90%的任何百分比。要注意70%配对碱基的百分比被认为有同源性,具有这个程度配对碱基对的杂交被认为是严格的。这种严格杂交的杂交条件可以从分子生物学现代试验方案(John Wiley和Sons,Inc.,1987)获得。更加特别地,杂交条件如下:
·例如在5x SSPE,5x Denhardt′s试剂,0.1%SDS,100g/mL剪切的DNA中在68℃进行的杂交
·0.2xSSC,0.1%SDS中在42℃的中等严格清洗
·0.1xSSC,0.1%SDS中在68℃的高严格清洗
有50%GC含量的基因组DNA具有大约96℃的TM。对于1%的错配,TM降低大约1℃。
另外,此处描述的任何进一步的杂交条件原则上也可应用。
当然,编码与如本发明鉴别的那些相同的多肽分子之所有核酸序列分子包括任何公开的给定编码序列,因为遗传密码的简并性可直接应用以清楚地测定编码给定多肽分子的所有可能的核酸分子,即使这样简并的核酸分子的数量可以很高。这个也可应用于给定多肽的片段,只要在免疫接种这点上所述片段编码适合用作例如作为主动或被动疫苗的多肽。
根据本发明的核酸分子作为第三备择方案也能是包含了根据上面总结的根据本发明的核酸分子第一和第二备择方案的核酸分子至少15个碱基的延伸的核酸。优选地,碱基形成了碱基连续的延伸。然而,所述延伸由许多碱基分开的两个或两个以上的基团组成,也在本发明的范围内。
本核酸优选地由此处公开的序列至少20个,甚至更加优选地至少30个,尤其是至少50个连续碱基组成。由于所用的计划区域(例如作为(PCR)引物、探针、(例如在(DNA)芯片上的)捕获分子等等),很容易地优化合适的长度。优选的核酸分子含有一个或多个表1和2中列出的推定的免疫原性氨基酸序列的至少连续15个碱基部分,尤其是表2的有高于10分,优选地高于20分,尤其是有高于25分的序列。尤其优选的是含有本应用的序列方案中任何序列的DNA序列连续部分的核酸,所述核酸与公开的粪肠球菌株V583基因组({Paulsen,I等人,2003};GenBank登陆号AE016830(染色体)、AE016833(pTEF1),AE016831(TE2),AE016832(pTEF3))和/或任何其它公开的粪肠球菌基因组序列或其部分,显示了一或多个,优选地多于2个,尤其是多于5个不相同的核酸残基。尤其优选的不相同的核酸残基是导致不同氨基酸残基的残基。优选地,核酸序列编码与上面提到的公开的粪肠球菌对应物具有至少1个,优选地至少2个,优选地至少3个不同氨基酸残基的多肽。这样分离的多肽也是优选的,所述多肽是此处例如序列列表中提到的蛋白质(或全蛋白质)的片段,具有至少6、7或8个氨基酸残基并由这些核酸编码。
根据本发明的核酸分子作为第四备择方案也可是在严格杂交条件下退火于根据上面总结的第一、第二和第三备择方案的本发明任何核酸的核酸分子。严格杂交条件一般是此处描述的那些。
最后,根据本发明的核酸分子作为第五备择方案也可是由于遗传密码的简并性否则能杂交根据上面总结的第一、第二、第三和第四备择方案的根据本发明任何核酸分子的任何核酸分子之核酸分子。这种核酸分子指的是优选地根据本发明的核酸编码根据本发明的超免疫血清反应性抗原或其片段的事实。这种核酸对于根据本发明的核酸分子的检测,并因而对例如粪肠球菌的各个微生物的诊断以及与这种微生物有关的任何疾病或疾病状态尤其有用。优选地,杂交将在如上面描述的第四备择方案描述的严格条件下发生或进行。
如此处使用的核酸分子一般指的是任何核酸分子或脱氧核糖核酸分子,可以是非修饰的RNA或DNA或修饰的RNA或DNA。因而,例如,如此处使用的核酸分子在其它之中指的是单和双链DNA,单和双链RNA的混合物的DNA,单和双链区域的混合物的RNA,包含可以是单链、或更加一般地双链、或三链、或单和双链区域混合物的DNA和RNA的杂交分子。另外,如此处使用的核酸分子指的是包含RNA、或者DNA、或者RNA和DNA的三链区域。这样区域的链可以来自相同的分子或来自不同分子。这些区域可以包括所有的一个或多个分子,但更一般地只涉及一些分子的区域。一个三螺旋区域的分子通常是寡核苷酸。如此处使用地,术语核酸分子包括如上面描述的含有一个或多个修饰碱基的DNA或RNA。因而,有为了稳定性或为了其它原因而修饰的骨架的DNA或RNA是此处术语所指的“核酸分子”。另外,包含例如次黄嘌呤核苷的异常碱基或例如三苯甲基化碱基的修饰碱基的DNA或RNA(只提及两个例子)是如此处使用的术语的核酸分子。将理解已经对有本领域技术人员已知的很多有用目的之DNA和RNA进行非常多种的修饰。如此处采用的术语核酸分子包含这样化学、酶或代谢修饰形式的核酸分子,以及病毒和其中包括简单和复杂细胞的细胞特征的化学形式的DNA和RNA。术语核酸分子也包含通常指的是寡核苷酸的短核酸分子。“多核苷酸”和“核酸”或“核酸分子”通常此处交替使用。
本发明提供的核酸分子也包括众多的独特的片段,比粪肠球菌编码区域的序列列表中列出的核酸分子序列更长和更短,能通过标准克隆方法产生。为了是独特的,片段必须有足够的大小使其能从其它已知的核酸序列中区分出来,最容易通过将任何挑选的粪肠球菌片段与例如GenBank的计算机数据库中的核苷酸序列比较而测定。
另外,能对本发明包含的核酸分子和多肽进行修饰。例如,能进行不影响核酸编码的多肽的核苷酸替代,因而编码超免疫血清反应性抗原或其片段编码的任何核酸分子也包含在本发明内。
此外,使用例如标准克隆技术的标准技术能将编码本发明提供的超免疫血清反应性抗原或其片段的任何核酸分子功能性地联接于任何期望的调节序列,不管是粪肠球菌调节序列或异源性调节序列、异源性先导序列、异源性标记序列或产生融合蛋白的异源性编码序列。
本发明的核酸分子可以是例如mRNA或cRNA的RNA形式,或者包括例如通过克隆得到的或化学合成技术产生的cDNA和基因组DNA或其组合的DNA形式。DNA可以是三链、双链或单链。单链DNA可以是编码链,也已知为正义链,或者它可以是非编码链,也指的是反义链。
本发明还涉及此处上面描述的核酸分子的变异体,所述变异体编码具有序列列表中列出的推算的粪肠球菌氨基酸序列的超免疫血清反应性抗原和其片段的片段、类似物和衍生物。核酸分子的变异体可以是天然发生的变异体,例如天然发生的等位基因变异体,或者它可以是不知道是天然发生的变异体。可以通过诱变技术制造核酸分子这样非天然发生的变异体,包括应用于核酸分子、细胞或生物体的那些。
在这点的变异体之中,变异体是不同于前面提到的核酸分子,差别在于有核苷酸替代、删除或增加的核酸。替代、删除或增加可包括一个或多个核苷酸。可在编码或非编码区或者这两个区域改变变异体。编码区的改变可产生保守或非保守氨基酸替代、删除或增加。优选的是编码变异体、类似物、衍生物或片段或者片段的变异体、类似物或衍生物的核酸分子,所述核酸分子具有如序列列表中列出的粪肠球菌序列,其中以任何的组合形式,数个、少数、5至10个、1至5个、1至3个、2个、1个或没有氨基酸被替代、删除或增加。这些之中尤其优选的是沉默替代、增加或删除,它们不改变序列列表中列出的粪肠球菌多肽的特性和活性。在这点也尤其优选的是保守替代。
根据本发明的肽和片段也包括修饰的表位,其中优选地根据例如{Tourdot,S.等人,2000}中公开的法则修饰或替代给定的表位的一个或两个氨基酸,以及编码这样修饰的表位的核酸序列。
很清楚根据本发明的表位涵盖了通过改进、保存或至少不显著阻碍表位的T细胞激活能力的氨基酸交换而衍生于本表位的表位。所以,本表位也涵盖了不含有源于粪肠球菌的原始序列但是激发相同或优选地改进的T细胞反应的表位。这些表位指的是“不规则变化的”;它们需要具有对MHC/HLA分子相似的或优选地对更大的亲和力,并且它们需要以相似或优选地更强的方式刺激指向原始表位的T细胞受体(TCR)的能力。
能通过合理设计获得不规则变化的表位,即考虑如例如{Rammensee,H.等人,1999}描述的单个残基对结合于MHC/HLA的作用,联合可能与TCR相互作用的残基的系统交换以及用指向原始表位的T细胞测试得到的序列。这样的设计对于的本领域技术人员是可能而无需过度的试验。
另一个可能性包括用针对原始表位的T细胞对肽库的筛选。优选的方式是合成肽库的位点扫描。这样的方法已经在例如{Hemmer,B.等人,1999}有详细描述并且此处给予参考。
作为本衍生氨基酸序列代表的表位或不规则变化的表位的备择方案,也能采用模仿这些表位的物质,例如“肽模仿物”或“回复-反-肽”(retro-inverso-peptides)。
改进的表位设计的另一方面,是带有增加其刺激T细胞的能力的物质之制剂或修饰。这些包括T辅助细胞表位、脂质或脂质体或如WO 01/78767中描述的优选的修饰。
增加表位T细胞刺激能力的另一方法是带有免疫刺激物质的制剂,例如象白细胞介素-2、-7、-12、-18、I和II型干扰素(IFN)的细胞因子或化学因子,尤其是IFN-γ、GM-CSF、TNF-α、flt3配体等。
另外如此处讨论地,关于本发明的核酸分子试验,例如如上面讨论的本发明的核酸分子可以用作RNA、cDNA和基因组DNA的杂交探针以分离编码本发明多肽的全长cDNA和基因组克隆,以及分离与本发明核酸分子具有很高的序列相似性的其它基因的cDNA和基因组克隆。这样的探针一般包含至少15个碱基。优选地,这样的探针将具有至少20个、至少25个或至少30个碱基,可以具有至少50个碱基。特别优选的探针将具有至少30个碱基,将具有50个或更少的碱基,例如30、35、40、45或50个碱基。
例如,使用已知的DNA序列通过筛选相关文库分离本发明的核酸分子的编码区域以合成寡核苷酸探针。具有互补于本发明基因序列的序列的标记寡核苷酸然后用于筛选cDNA、基因组DNA或mRNA文库以测定探针杂交于文库的哪些成员。
如此处进一步讨论地,关于核酸分子试验,本发明的核酸分子和多肽可以用作开发尤其是人类疾病的疾病的治疗和诊断的试剂和物质。
作为寡核苷酸的本发明的核酸分子能用于此处描述的方法中,但是优选地是PCR,以测定此处鉴别的粪肠球菌基因是否全部或部分存在于例如血液的感染组织和/或在其中转录。已经认识到这样的序列也将在病原体已经达到的感染阶段和感染类型的诊断有用途。为了这个和其它的目的,可以使用如此处描述的包含至少一个根据本发明的核酸的阵列。
根据本发明的核酸分子可以用于核酸分子和含有这些核酸的生物体或样品的检测。优选地这样的检测是为了诊断,更加优选地是为了与粪肠球菌的存在或丰度相关的或相联的疾病的诊断。
感染了粪肠球菌的特别是哺乳动物尤其是人的真核细胞(此处也是“个体”)是可以鉴别的,方法是通过各种技术在DNA水平检测的根据本发明的任何核酸分子的检测。能够获得为了从其它生物体区分粪肠球菌的优选的候选核酸分子。
本发明提供了诊断源自粪肠球菌感染的疾病的方法,包含从个体分离的或来源于个体的样品测定具有序列列表中列出的核酸分子序列的核酸分子表达水平的增加。能使用本领域中众所周知的核酸分子定量的方法测量核酸分子的表达,例如PCR、RT-PCR、RNase保护、Northern斑点、其它的杂交方法和此处描述的阵列。
如此处使用的分离的是指“通过人工”从其天然状态分离;即,如果它天然发生,它将被改变或从其原始环境去除,或两个都有可能。例如,天然存在于活的生物体的天然发生的核酸分子或肽在其天然状态是不“分离的”,但是从其天然状态的共存物质中分离的相同的核酸分子或多肽是“分离的”,如此处采用的术语。作为分离的部分或下面的分离,例如为了诱变形成融合蛋白质以及为了宿主中的增殖或表达,这样的核酸分子能连接于例如DNA的其它的核酸分子。分离的核酸分子单独或连于例如载体的其它核酸分子能被引入培养的或全生物体的宿主细胞。引入培养的或全部生物体的宿主细胞,这样的DNA仍然将是分离的,如此处使用的术语,因为它们将不是它们天然发生的形式或不在它们天然发生的环境中。相似地,核酸分子和多肽可以发生在组合物中,例如介质制剂、核酸分子或多肽引入例如细胞内的溶液、化学或酶反应的组合物或溶液,例如,它们不是天然发生的组合物,在此处采用的那个术语的意义之内,在那里仍然是分离的核酸分子或多肽。
根据本发明的核酸可以是化学合成的。或者,核酸能通过本领域的技术人员已知的方法从粪肠球菌中分离。
根据本发明的另一方面,通过使用此处描述的抗原鉴别方法提供了一套全面的新型超免疫血清反应性抗原和其片段。本发明优选的实施方案中,提供了包含此处描述的任何一个核酸分子编码的氨基酸序列的超免疫血清反应性抗原及其片段。本发明另一优选的实施方案中,提供了包含选自Seq ID No 171-172,174-178,180,182-188,190-193,195-196,199-213,215-232,234-244,246-247,249-253,255-259,261-262,264-284,287-296,298-316,318-340,425,427,431-433,439,444,446,449-451,459-460,462-463和467-476中表示的多肽序列之氨基酸序列的一套新型超免疫血清反应性抗原及其片段。本发明另一优选实施方案中,提供了包含选自Seq ID No 173,179,181,194,197,214,233,245,254,285-286,297,426,428-430,434-438,440-443,445,447-448,452-458,461和464-466中表示的多肽序列之氨基酸序列的一套新型超免疫血清反应性抗原及其片段。在本发明的又一实施方案中,提供了包含选自Seq ID No 260,317中表示的多肽的氨基酸序列之超免疫血清反应性抗原及其片段。
如本发明提供的超免疫血清反应性抗原及其片段包括序列列表中列出的任何多肽,以及如下的多肽:具有与序列列表中列出的多肽至少70%相同性,优选地与序列列表中列出的多肽至少80%或85%相同性,更加优选地与序列列表中列出的多肽至少90%相似性(更加优选地至少90%相同性)以及仍然更加优选地与序列列表中列出的多肽至少95%、96%、97%、98%、99%或99.5%相似性(仍然更加优选地至少95%、96%、97%、98%、99%或99.5%相同性),也包括这样的多肽的部分,所述多肽一般有含有4个氨基酸,更加优选地至少8个,仍然更加优选地至少30个,仍然更加优选地至少50个氨基酸,例如4,8,10,20,30,35,40,45或50个氨基酸的这样的多肽部分。
本发明还涉及这些超免疫血清反应性抗原及其片段的片段、类似物和衍生物。术语“片段”、“衍生物”和“类似物”当指的是其氨基酸序列列于序列列表中的抗原时,是指基本上保留了与这样的超免疫血清反应性抗原及其片段相同的或相似的生物功能或活性的多肽。
超免疫血清反应性抗原及其片段的片段、衍生物或类似物可以是1)其中一个或多个氨基酸残基被保守或非保守氨基酸残基(优选地保守的氨基酸残基)替代的一种,并且这样替代的氨基酸残基可以是或可以不是遗传密码编码的一个,或者2)其中一个或多个氨基酸残基包括取代基团的一种,或者3)其中成熟的超免疫血清反应性抗原及其片段与例如增加超免疫血清反应性抗原及其片段半衰期的化合物(例如,聚乙二醇)的另一种化合物融合的一种,或者4)其中额外的氨基酸融合于成熟的超免疫血清反应性抗原及其片段的一种,例如先导或分泌序列或用于成熟超免疫血清反应性抗原及其片段的纯化的序列或前蛋白质序列。这样的片段、衍生物和类似物认为在本领域技术人员此处教义的范围内。
本发明也涉及不同的粪肠球菌分离株的抗原。基于此处公开的核酸和氨基酸序列可以很容易地分离这样的同源物。对于每一个M血清型能相应地测定任何抗原的存在。另外,测定多种种血清型中特定抗原的变异性是可能的,如sic基因(Hoe,N.等人,2001)。多种血清型对不同肠球菌感染的作用在不同年龄组和地理区域是不同的。期望最有价值的保护性抗原在各种临床株之中是保守的是一个很重要的方面。
在这方面,序列列表中列出的超免疫血清反应性抗原及其变异体、类似物、衍生物和片段,以及片段的变异体、类似物和衍生物在本发明尤其优选的实施方案中。额外地,包含这样的超免疫血清反应性抗原及其变异体、类似物、衍生物和片段以及片段的变异体、类似物和衍生物的融合多肽也包含在本发明中。使用标准技术能很容易地制造这样的融合多肽和蛋白质以及编码它们的核酸分子,包括生产和表达编码融合蛋白质的重组多核酸的标准重组技术。
由于保守氨基酸替代而与参比不同的那些在优选变异体之中。这样的替代是多肽中给定氨基酸被另一个同样特性的氨基酸替代的那些。一般视为保守替代是脂肪性氨基酸Ala,Val,Leu和Ile中互相的取代;羟基残基Ser和Thr的交换,酸性残基Asp和Glu的交换,酰胺残基Asn和Gln之间的替代,碱性残基Lys和Arg的交换以及芳香基残基Phe和Tyr之间的取代。
在这点上进一步尤其优选的是具有序列列表中列出的任何多肽之氨基酸序列的变异体、类似物、衍生物和片段,以及片段的变异体、类似物、衍生物,其中几个、少数、5至10个、1至5个、1至3个、2个、1个或没有氨基酸残基以任何联合形式被替代、删除或增加。这些之中尤其优选的是不改变本发明多肽的特性和活性的沉默替代、增加和删除。在这点上也尤其优选的是保守替代。最高度优选的是具有序列列表中列出的没有替代的氨基酸序列的多肽。
优选地以分离的形式提供本发明的超免疫血清反应性抗原及其片段,优选地是纯化为同质的。
包含具有序列列表中列出的氨基酸序列的多肽的片段以及序列列表中列出的多肽的变异体和衍生物的片段也在本发明优选实施方案中。
在这点上,片段是具有与前面提到的超免疫血清反应性抗原及其片段的部分但不是所有氨基酸序列完全相同的氨基酸序列的多肽及其变异体或衍生物、类似物、片段。这样的片段可以是“游离的”,即不是其它氨基酸或多肽的部分或者与其融合,或者它们也包含在它们形成的一部分或区域的更大的多肽内。本发明这个方面也优选的是特征在于本发明多肽的结构或功能属性的片段,即包含本发明多肽的α-螺旋和α-螺旋形成区域、β-折叠和β-折叠形成区域、折转和折转形成区域、卷曲和卷曲形成区域、亲水区域、疏水区域、α两性区域、β两性区域、可曲(flexible)区域、表面形成区域、底物结合区域和高抗原指示区域的片段以及这样的片段的联合。优选的区域是介导本发明超免疫血清反应性抗原及其片段的活性的那些。在这点上最高度优选的是具有本发明超免疫血清反应性抗原及其片段的化学、生物或其它活性的片段,包括有相似活性或改进活性或有降低的非期望活性的那些。尤其优选的是包含酶受体或结构域的片段,所述酶受体或结构域赋予粪肠球菌存活或造成人类疾病的能力所必须的功能。进一步优选的多肽片段是包含或含有尤其是人的动物中抗原或免疫原性决定簇的那些。
抗原片段定义为鉴别的抗原的片段,对于其本身它是抗原的或者当提供为半抗原时可以制成抗原的。所以,本发明实现了显示一个或(对于更长的片段)只有少数氨基酸交换的抗原或抗原片段,假设在交换时没有严重地破坏有氨基酸交换的这样的片段的抗原能力,即适于引发接受这个抗原免疫接种的个体的合适免疫反应以及用来自个体血清的个体抗体制备物鉴别。
超免疫反应抗原这样的片段的优选实例选自包含表1a和表1c的“推定的免疫原性氨基酸”栏和“鉴别的免疫原性区域的位置”的氨基酸序列的多肽,表2的血清反应性表位,尤其是包含如下氨基酸的多肽:4-10,14-21,30-36,59-68,77-82,87-93,96-105,112-121,125-133,135-141,150-162,164-183,192-203,207-213,215-226,228-234,241-247,250-285,302-308和135-148位的Seq ID No 171;15-57,60-73,77-101,108-134,136-177,185-201,203-217,226-240,244-254,272-277,283-288,292-343,354-370,380-398,406-437,439-453,473-490,532-538,584-590,595-601,606-612,664-677,679-704,715-724,731-753,759-772,786-794,814-862和657-684位的Seq ID No 172;4-9,15-36,41-47,54-60,75-81,114-120,131-146,152-158,174-182,194-202,208-215,218-226,255-271,276-285,290-295,302-311,318-328,330-344,352-359,365-377,388-395,398-405,426-432,439-449,455-500,505-513,531-537,542-552,554-561,587-595,606-612,718-734,763-771,775-782,792-801,805-812,822-828,830-843,849-863,876-894,905-911,919-926,935-947,949-958,968-979,1009-1016,1029-1045,1047-1056,1076-1081,1092-1106,1123-1133,1179-1200,1202-1211,1215-1223,1287-1299,1301-1306,398-431和1224-1237位的Seq 1D No 173;17-47,74-80,90-97,126-133,137-148,167-173,179-185,214-223,250-255,270-283,329-338,342-350,352-358,360-367,372-383,398-404,411-421,426-432,435-446,452-462,472-479,515-521,582-592,611-618,623-629,642-659,666-673,678-689,704-725,732-737,744-757,768-789,824-834,842-849,862-868,877-887,904-916,923-928,941-947,962-974,982-992,1019-1030,1032-1044,1046-1052,1065-1075,1077-1087,1108-1121,1124-1132,1137-1151,1170-1182,1190-1206,1208-1214,1227-1233,1242-1251,1254-1273,1282-1298和792-825位的Seq ID No174;19-31,39-67,82-91,104-110,113-128,149-155,161-181和137-155位的Seq ID No 175;6-18,54-63,69-85,110-127,142-156,158-167,169-211,238-246,248-257,276-311,339-349,371-380,385-391,394-403,421-438,451-456,483-489和449-468位的Seq ID No 176;5-15,24-34,50-56,61-83,98-121,123-136,149-162,166-194,202-215,221-227,229-332,337-360,367-402,404-415,427-433,444-462,471-478,487-498,511-518,521-544,550-563,568-574,580-587,597-607,610-616,624-629和468-498位的Seq ID No 177;11-19,32-49,57-63,65-71,80-89,91-133,166-181,183-191,201-230,234-257,264-291,297-303,305-314,316-335,337-354,359-366,368-374,383-388,394-405,408-442,446-470,483-490,499-505,513-538,544-555,557-563,568-590,598-608,617-623,627-636,641-647,667-685,687-693,710-723,733-739,742-754,769-815和366-388位的Seq ID No 178;4-16,30-35,42-53,67-76,82-87,101-108,112-130,132-138,147-152,161-183,187-208,218-225,265-281,295-303,305-317,322-334,338-357,360-368,370-383,387-394,400-419,421-430和255-336位的Seq ID No 179;19-27,36-47,59-66,76-83,101-112,118-125,142-147,162-180,185-196,225-240,246-263,286-304,314-319,327-333,353-367和194-214位的Seq ID No 180;14-43,70-76,83-89,111-117,122-128,136-145,163-170,175-182,210-219,246-251,266-279,325-331,338-346,348-354,356-363,368-379,422-428,431-441,450-456,466-473,509-515,532-542,549-556,576-586,605-612,617-623,636-653,660-667,674-686,698-719,726-731,738-745,762-783,818-828,836-843,856-862,871-881,903-910,917-922,935-941,956-968,976-986,1013-1024,1026-1038,1059-1069,1071-1081,1102-1115,1118-1126,1131-1145,1164-1176,1187-1200,1202-1208,1221-1227,1236-1245,1248-1267,1273-1292,252-287和805-844位的Seq ID No 181;4-18,21-28,37-43,56-70,101-113,131-140,142-150,162-170,172-184,193-204,209-227,233-238,246-264和93-168位的Seq ID No 182;14-20,44-50,61-70,77-96,99-106,129-142,168-181,187-196,205-221,225-241,277-296和257-281位的Seq ID No 183;18-29,43-54,64-76,78-84,88-103,125-149,159-176,198-218,230-242,256-271,279-285,287-293,300-306,325-331,344-351,357-364,371-397,400-414,419-464,485-515,517-526,529-537,548-553,573-580,584-590,603-620,639-661,676-681,687-700,716-761,772-780,785-790,795-803,823-836,848-853和106-134位的Seq ID No 184;7-13,19-42,44-51,55-75,87-97,99-110,112-118,129-135,141-156,158-178,213-220,230-286,294-308,323-338,345-352,355-365,370-392,394-419,437-446,454-460,4741497,515-526,528-546,569-575和128-141位的Seq ID No 185;12-20,24-33,45-70,73-84,86-94,103-116,118-124,135-142,163-170,176-200,202-224,226-234,237-248,250-262,265-287,296-307,334-341,347-356,361-369,382-396,405-415,418-427,431-439,443-449,452-461,467-474和113-146位的Seq ID No186;13-38,44-50,52-59,66-72,83-94,103-110,116-124,131-137,158-180,199-204,218-233,241-264,269-317,326-342,350-356和70-86位的Seq ID No 187;29-35,49-59,63-84,86-97,103-111,113-126,130-144,150-158,174-198,221-231,250-264,266-273,291-298,310-318和70-90位的Seq ID No 188;19-25,28-52,60-66,71-76,131-142,149-155,157-178,181-213,218-223,237-242,250-257,260-266,272-279,282-290,321-330,373-385,393-407,441-453,461-475,509-521,529-542,577-589,597-610,643-655,663-677,703-718,729-734,358-464,495-570和604-685位的Seq ID No 189;4-29,51-76,116-136,158-173,179-193,207-215和86-111位的Seq IDNo 190;5-23,45-70,79-90,93-107,114-122,142-151和18-36位的SeqID No 191;9-51,68-120,133-149,158-180,186-206,211-220,222-237,248-293,296-310,317-339和248-260位的Seq ID No 192;14-24,44-63,69-98,108-119,123-136,155-161,164-176,180-193,203-208,215-223,239-247,274-281,283-289,296-304,306-313,315-327,331-341,343-353,357-386,392-405和205-246位的Seq ID No 193;5-13,16-23,36-42,53-63,70-83,96-102和14-34位的Seq ID No 194;4-13,19-35,49-56,59-76,83-107,121-134,144-153,157-164,166-186,194-202,209-216,231-253,257-264和98-134位的Seq ID No 195;16-32,38-47,58-68,78-89,98-114,117-123,132-141,146-156,164-170,179-188,196-212,219-230,232-237,244-263,265-274,278-293,297-303,306-326,339-349,352-359,362-367,373-379,384-394,396-406,423-443,451-461,465-484,490-497,504-511,523-533,537-547,550-556,558-566,573-579,586-593,598-609,615-642,647-665,671-686,693-713,723-728和332-378位的Seq ID No 196;6-21,34-44,58-64,66-74,79-87,114-127,129-143,154-162,174-189,205-214,241-262,266-273,278-297,319-324,328-338,342-351,390-398,409-415,422-435,458-464,471-477,481-486,506-531,534-540,542-550和315-389位的Seq ID No 197;4-28,39-45,52-58,69-82,93-115,122-128,135-140,146-163,177-192,209-215,221-232,271-284,331-337,341-352,360-378,383-390,392-401,409-422,428-435,462-470,474-480,482-496,531-539,541-549,551-560,562-569,576-582,598-618和98-127位的Seq ID No 198;14-27,33-47,61-79,94-104,119-133和36-60位的Seq ID No 199;11-22,29-40,48-62,68-73,96-106,108-118,125-149和102-126位的Seq ID No 200;4-11,45-55,76-83,86-102,105-112,138-144,147-153和20-48位的Seq ID No 201;12-20,28-56,62-68,72-82,93-99,101-107,120-133,135-145,178-186,208-232,279-292和36-64位的Seq ID No 202;6-14,23-48,65-82,92-134,140-181,188-219,228-238,244-253,255-261和124-145位的Seq ID No 203;11-25,31-38,53-59,62-71,89-99,125-133,151-157,182-190,195-203,208-215,219-229,249-262,267-275,287-295,298-316,318-325,328-334,344-353,357-363,371-377,385-391,396-415,425-436,438-457,471-485,538-552,554-561,606-625,630-636,646-653,669-679,695-704,706-715,722-747,763-773和714-738位的Seq ID No 204;10-29,33-45,50-60,70-79,83-95,118-124,136-157,176-184,192-205,207-216,223-234,240-246,258-268,275-283和37-56位的Seq ID No 205;4-24,27-38,46-54,66-72,81-97,112-119,128-137,152-157,173-179,185-214,219-225,227-248,262-284,286-295,301-307和117-134位的Seq ID No 206;26-43,49-56,60-71,74-82,87-98,110-116,131-146,154-164,169-178,183-189,205-214,241-246,255-268,275-292,305-314,316-323,326-340,346-363,397-402,419-429,440-446,452-461,467-475和29-66位的SeqID No 207;7-16,21-39,48-58,61-78,82-89,109-136,138-150,152-176,182-247,255-261,267-332,336-345,347-358,362-368,371-392,394-404,407-472,490-498,505-513,527-544,554-582,603-611,614-620,632-638和500-523位的Seq ID No 208;24-46,77-83,90-97,99-118,123-166,168-177,204-212,229-239,248-262,273-282,287-293,300-319,321-337,340-352,357-366,391-402,411-428,442-450,464-471,479-489和19-40位的Seq ID No 209;9-23,25-34,53-58,70-86,90-97,99-116,118-128,131-141,185-191,228-233,237-253,255-261,264-271,273-280,302-312,319-349,351-359,362-369,376-383,387-394,398-406,419-434和20-31位的Seq ID No 210;15-22,37-43,71-87,105-115,121-127,135-142,152-158和32-52位的Seq ID No 211;6-12,18-29,37-47,50-58,65-83,85-91,94-99,108-123,142-150,156-163,183-193,215-222,242-249,252-258,261-270,285-308,318-326和1-95位的Seq ID No 212;9-61,65-133,144-155,166-173,175-221,233-276,278-313,329-368和210-233位的Seq ID No 213;11-29,33-39,46-51,65-93,107-113,134-143,147-154,166-177,181-188,214-220,233-243,263-269和112-128位的Seq ID No 214;8-46,110-134,155-167,174-183,188-201,210-230,253-258,267-282,289-299,312-319,322-327,330-337,365-381,389-402,405-411,419-425,439-447,465-472,489-512,525-532,540-554,577-589,591-599,605-614,616-624,633-649和503-529位的Seq ID No 215;34-49,64-70,90-118,124-131,141-152,159-165和112-128位的Seq IDNo 216;5-15,26-45,55-72,80-85,93-100,121-133,142-148,154-167,198-205,209-215,241-254,260-265,271-279和244-270位的Seq ID No217;4-36,38-54,67-83,122-153,159-178,205-212,232-242,244-253,259-268,281-288,298-309,324-331,334-370,372-381,389-401,403-429,441-450,456-462,465-471,473-479,483-504,508-518,537-543,553-565,578-584,592-609,619-625,658-667,669-679,712-719,722-729,737-744,746-752,758-765和180-226位的Seq ID No218;6-17,23-32,49-56,61-67,76-83,85-103,105-111,120-132,145-171,175-185,191-225,231-246和99-128位的Seq ID No 219;4-24,28-48,52-58,64-79,87-100,104-120,136-152,159-166和150-163位的Seq ID No 220;15-27,65-71,77-99,104-121,128-154,183-216,223-229,234-255,277-287,296-308和77-97位的Seq ID No 221;8-18,44-76,102-109和49-57位的Seq ID No 222;5-14,28-40,42-51,54-60,77-83,89-100,117-124,146-172,176-204,216-231,237-244,267-278,324-334,342-348,396-401,427-433,438-450,452-457,465-471,473-481,491-500,509-515,523-544,550-556,558-569,589-595,606-618,625-632,640-649,665-671,678-688,691-698,717-723,728-734,781-789,800-805,812-821,833-868,873-879,889-905,929-939,988-998,1046-1061,1073-1079,1089-1096,1115-1124,1132-1140,1172-1196,1220-1226,1231-1249,1269-1277,1287-1301,1307-1330,1350-1361,1369-1378,1387-1412,1414-1420,1422-1439,1484-1491,1513-1529,1552-1561,1576-1583,1606-1613,1617-1640,1647-1654,1665-1679,1686-1698,1709-1727,1736-1743,1750-1757,1771-1790,1801-1807,1817-1823,1831-1842,1859-1868,1870-1882,1884-1891,1900-1906,1909-1914,1929-1935,1952-1960,1974-1988,2002-2011,2032-2063,2071-2081,2116-2124,2139-2147,2149-2159,2163-2190,2209-2215,2245-2253,2282-2287,2331-2342,2360-2370,2379-2393,2402-2408,2414-2421,2423-2430,2433-2439,2442-2450,2472-2478,2485-2493,2495-2503,2506-2512,2547-2554,2558-2564,2615-2625,2637-2652,2692-2698,2700-2706,2711-2723,2731-2740,2748-2753,2756-2762,2765-2772,2781-2798,2810-2824,2844-2852,2885-2899,2912-2922,2937-2944,2947-2970,2988-2998,3016-3025,3032-3037,3062-3071,3129-3148,3156-3161和530-607位的Seq ID No 223;31-36,57-62,79-85,90-96,99-112,120-146,162-185,193-203,208-217,219-226,239-253,283-290,298-304,306-321,340-349,351-361,365-372,386-395,407-438,473-486,537-551,558-568,576-594,598-604和75-95位的Seq ID No 224;14-19,24-30,34-42,45-52,54-64,66-82,95-105,107-118,126-163,171-177,184-201,210-215,260-269,273-279,288-304,321-327,358-364,370-375,380-387,394-404,407-413,421-431,436-451,465-474,504-511,531-552,578-587,614-626,629-636,638-671,691-715,719-729,733-745,752-759,768-777,785-792,794-802,805-824,844-854,867-880,885-891,893-902,907-924,939-948,955-964,966-975,987-1000,1012-1017,1023-1028,1050-1071,1083-1098,1102-1115,1133-1146,1170-1183,1204-1211,1213-1223,1262-1311,1313-1319,1346-1355,1366-1371,1383-1405,1409-1414和776-819位的Seq ID No 225;12-27,30-38,54-61,64-74,82-96,103-110,117-125,134-140,147-158,185-201,218-225,232-253,265-280,319-325,350-362,366-372,376-386,464-483,485-490,511-521,531-537,542-559,564-574,593-609,613-619,637-642,668-677和195-214位的Seq ID No 226;4-21,59-67,73-79,84-91,141-151,186-197,203-214,222-227,237-245,255-260,281-292,294-311,336-344,346-355,422-437,459-466,484-491和77-109位的Seq ID No 227;10-45,52-61,63-70,74-102,112-122,124-132,164-178,181-205,212-240,246-256和226-247位的Seq ID No228;38-50,53-63,78-87,89-111,126-152,169-176,179-186,193-228,254-267,275-282,288-304,309-318,325-341,346-353,358-367,384-395,404-427,429-435,456-465,467-501,510-521,523-536,541-548,552-560,563-584,589-595,597-620,625-637,639-645,661-666,712-729,734-741,743-750,775-806,809-816,818-840,842-850和693-714位的Seq ID No 229;5-17,30-37,52-75,77-86,88-107,112-135,151-160,178-222,226-246,263-270,279-294,306-314,327-342,345-352,374-381,389-416,422-429,435-449,453-467,473-500,512-522,524-531,542-549,552-560,565-571,575-586,594-600,613-619,625-633,635-641,647-653,667-674,680-699,711-729,735-741,764-775,781-786,792-798,805-813,817-825,833-842,850-855,860-866,869-910,917-930,949-990和533-562位的Seq ID No 230;7-14,39-46,61-74,83-89,93-99,110-121,136-150,172-180,182-200,207-216,223-236,238-251,265-271,280-288,294-309,320-336,339-354,362-377,383-389,401-407,435-441,446-453,460-465,472-487,499-511,518-528,533-540,557-570,572-587,631-637,643-658,663-669,672-678,681-687,695-706,714-728和118-139位的Seq ID No 231;5-19,24-30,56-64,69-79,93-100,102-111,117-123,125-133,174-182,185-199,205-224,268-275,311-336和102-125位的Seq ID No 232;6-35,39-45,57-62,80-85,92-106,117-122,126-171,214-223,253-260,268-273,285-291,295-306,315-320,325-336,361-366和172-202位的Seq ID No 233;4-13,24-37,45-51,58-66,84-92,112-121,132-141,151-171,175-195,204-212,222-240,262-268,276-295,305-336,338-348,354-362和160-183位的Seq ID No 234;10-16,24-35,41-73,78-104,111-121,124-139,141-148,150-164,196-215,224-241,249-282,299-307,315-357,368-378,393-401和345-367位的Seq ID No 235;4-32,48-53,61-67,84-104,112-118和106-130位的Seq ID No 236;21-28,31-36,65-81,98-105,115-121,123-131,136-142,155-161,177-190和201-232位的Seq ID No237;4-15,21-27,33-39,42-56,58-64,68-82,84-90,92-98,113-122,146-162,168-175,177-189,191-203,249-268,279-285,287-304,328-342,349-358,371-378,387-393,404-413,419-425,467-479,487-498,513-524,528-539,541-565,572-579,595-606,626-635,637-642和612-626位的Seq ID No 238;7-13,52-70,76-82,97-106,110-117和13-45位的Seq ID No 239;5-10,12-48,59-64,87-102,107-128,131-140,154-161,165-171,173-215和54-74位的Seq ID No240;4-11,19-28,34-40,74-81,87-98,126-147,163-171,184-193,205-213和49-124位的Seq ID No 241;7-14,23-29,35-40,61-67,99-106,111-122,124-133,135-161,187-206,216-229,236-245,262-268,271-280和256-273位的Seq ID No 242;4-13,17-37,47-54,85-99,105-113,120-132,147-166,180-186,192-199,204-216和127-144位的Seq ID No 243;14-27,29-37,52-62,68-76,89-96,117-123,125-131,137-145,166-195,205-212,214-222,228-235,258-264,271-281,288-296,308-324,332-339,355-361,365-371和268-293of Seq ID No244;4-21,30-42,54-60,78-85,90-110,141-147,160-168,176-185,194-206,218-225,230-245,251-261,287-293,295-304,320-326,334-347,351-362,386-402,413-423,427-433,439-453,456-477,480-493,507-513,526-539,574-581,591-598,600-609,614-632,655-665,685-691,703-712,742-747,757-775,797-803,813-819,823-829,880-887,901-906,930-944,948-958,962-968,971-995,1002-1009,1017-1023,1036-1053,1069-1081,1107-1124,1129-1152,1178-1195,1211-1223,1249-1266,1271-1288,1334-1340,1346-1367,1-63和171-189位的Seq ID No 245;4-22,52-63,70-75,94-104,112-125,133-141,176-199,209-216,244-259,287-299,336-352,366-372,386-399,421-436,444-449,457-466,481-487,506-529,531-540和295-378位的Seq ID No 246;9-30,43-49,58-75,86-96,119-131,138-147,162-167,181-201,208-214和16-121位的Seq ID No 247;4-27,52-58,80-90,92-100,108-114,118-143,169-176,189-198,247-261,281-287,307-317,323-329,352-363,372-381,396-411,413-426,429-440,442-450,456-461,468-479和1-73位的Seq ID No 248;4-32,47-52,57-63,71-78,92-104,126-142,153-175和145-163位的Seq IDNo 249;17-23,35-41,51-70,73-86,104-125和105-129位的Seq ID No250;25-32,41-50,75-85,87-103,115-122,138-149,164-171,188-210,212-220,224-234,256-273,288-299,304-310,330-336,357-365,382-390,399-405,414-421,440-446,454-461,480-486,502-514,518-540,543-553,561-567,572-580,582-588,595-630,633-651,672-681,691-709,760-767,813-832,841-848,852-866,873-893,919-925,927-933,940-955,957-978,984-997,1000-1010,1035-1040,1044-1051,1058-1064,1081-1091,1097-1124,1129-1138,1144-1150,1158-1165,1170-1180,909-936和1001-1031位的Seq ID No 251;4-12,19-26,31-41,49-64,66-86,101-117,119-127,134-142,152-161,163-172,179-188,209-218,234-241,276-291,294-300,307-320,324-341,346-356,373-387,389-397,410-416,418-436,444-454,460-472,481-486,500-507,511-535,541-549,553-559,579-586,602-607,613-620,628-640,654-663,671-678,681-691,709-722,741-754,766-774,778-786,797-803和212-226位的Seq ID No 252;4-10,15-27,34-54,60-73,79-88,101-115,120-136,154-162,167-172,222-240和126-195位的Seq ID No 253;5-16,18-25,29-35,57-63,86-91,107-121,123-131,170-179,185-199,204-226,250-255,262-274,291-296,325-347和1-38位的Seq ID No 254;7-19,22-34,36-42,48-54,60-66,71-76,104-110,118-133,135-145,158-164,167-174,182-193,196-204,217-229,251-290,293-299,309-315和288-318位的Seq ID No 255;43-51,55-61,66-73,80-90,103-127,133-142,174-180,185-196,203-210,229-235,239-251,258-266,272-278,289-314,316-326,340-346,355-361和14-27位的Seq ID No 256;4-25,27-33,35-41,52-74,76-89,99-124,138-144,146-159,167-182,184-191,193-206,211-223,232-240,249-257,270-279,281-287,293-310,322-341,347-356和292-322位的Seq ID No 257;5-13,28-38,43-60,67-72,98-116,122-134,137-151,167-174,177-195,197-216和99-195位的Seq ID No 258;15-33,35-42,48-57,62-68,73-91,107-119,121-153,173-194,205-210,223-228,234-241,243-259,275-298,308-315,327-340,342-370,376-391,398-404,410-419和71-122位的Seq ID No 259;12-39,43-64,87-95,99-105,114-126,128-136,139-147,212-225和107-141位的Seq ID No260;6-33,40-45,60-75,79-86,121-129,131-137,161-167,172-178,186-195,203-212,236-244,257-264,278-294,306-312,345-358,368-381,386-395,404-410,412-418和198-270位的Seq ID No 261;18-31,34-41,50-56,69-83,99-106,129-141,147-153,159-168,170-178,190-198,200-212,221-232,237-255,261-266,274-292和118-216位的Seq ID No 262;17-47,61-67,87-93,115-121,126-132,140-148,167-173,179-186,214-223,250-255,264-272,282-294,306-318,338-353,358-377,385-401,414-420,433-441,451-457,470-480,505-511,544-550,571-581,600-607,612-618,631-648,655-662,669-681,693-714,721-726,733-740,757-778,813-823,831-838,851-857,866-876,893-905,912-917,930-936,951-963,971-981,1008-1019,1021-1033,1035-1041,1054-1064,1066-1076,1097-1110,1113-1121,1126-1140,1159-1171,1182-1195,1197-1203,1216-1222,1231-1240,1243-1262,1268-1287和738-828位的Seq ID No 263;19-28,40-46,51-57,68-74,81-87,98-108,111-121和20-36位的Seq ID No 264;4-17,19-44,60-69,80-86,110-116和33-60位的Seq ID No 265;8-16,18-28,42-50,53-75,79-86,94-99,122-128,136-142,149-163,166-173,198-212,254-272,288-295,304-318,324-329,343-348,351-364,367-383,389-395,411-420,427-436和11-56位的Seq ID No 266;19-25和6-24位的Seq ID No 267;6-39,59-68和44-63位的Seq ID No 268;5-14,21-28,38-53和29-41位的Seq ID No 269;4-13,31-41,56-65和32-56位的Seq ID No 270;5-12和4-21位的Seq ID No 271;4-18和17-32位的Seq ID No 272;4-10,23-33和14-30位的Seq ID No 273;26-34,44-53和35-52位的Seq ID No 274;1-19位的Seq ID No 275;4-17,23-30,32-37和6-23位的Seq ID No 276;5-33,40-58,61-66和45-66位的Seq ID No 277;15-41,61-67和41-65位的Seq ID No 278;4-12,16-23,26-37和10-29位的Seq ID No 279;23-39和37-55位的Seq IDNo 280;12-20和38-55位的Seq ID No 281;22-37和7-22位的Seq IDNo 282;3-14位的Seq ID No 283;6-16,43-65,71-76和17-31位的SeqID No 284;4-13,27-39,42-69和17-32位的Seq ID No 285;4-12,26-39和10-25位的Seq ID No 286;2-31位的Seq ID No 287;6-38,49-62和39-55位的Seq ID No 288;4-10,24-30和2-19位的Seq ID No 289;12-17,25-46和15-30位的Seq ID No 290;4-13和2-28位的Seq ID No291;30-38和17-45位的Seq ID No 292;24-33,55-61和31-61位的SeqID No 293;4-26,34-48和15-33位的Seq ID No 294;9-15和1-22位的Seq ID No 295;4-31和14-33位的Seq ID No 296;5-34,49-55,64-82和69-83位的Seq ID No 297;33-45和21-39位的Seq ID No 298;7-14,24-32,42-65,79-86和50-64位的Seq ID No 299;13-27,33-43,45-62和12-37位的Seq ID No 300;4-15,17-32和10-26位的Seq ID No 301;4-9,11-43,45-75和47-69位的Seq ID No 302;4-18,22-37和17-34位的SeqID No 303;4-14和5-24位的Seq ID No 304;7-33,35-46和1-19位的Seq ID No 305;13-37,69-75和51-69位的Seq ID No 306;14-24,26-34,37-49,66-78和2-25位的Seq ID No 307;17-46,52-57,59-64和54-68位的Seq ID No 308;4-22和13-25位的Seq ID No 309;8-40,53-63和29-50位的Seq ID No 310;16-28和32-40位的Seq ID No 311;14-20,22-28,39-45和2-22位的Seq ID No 312;4-13和12-31位的Seq ID No313;15-21和2-17位的Seq ID No 314;4-17和20-36位的Seq ID No315;4-19和9-18位的Seq ID No 316;4-14和3-19位的Seq ID No 317;4-21,32-40和21-39位的Seq ID No 318;4-13和10-27位的Seq ID No319;18-31,39-47,75-87,89-98和79-99位的Seq ID No 320;15-21和9-24位的Seq ID No 321;4-14,18-27,30-53,55-64,68-74,81-98和22-40位的Seq ID No 322;7-24,44-51和35-60位的Seq ID No 323;10-47和23-37位的Seq ID No 324;4-10,12-46和7-22位的Seq ID No325;20-27和1-13位的Seq ID No 326;6-19,41-51和9-37位的Seq IDNo 327;4-9,11-17和9-23位的Seq ID No 328;4-17,23-38,46-66,68-85和34-46位的Seq ID No 329;4-18,34-59,75-81和61-84位的SeqID No 330;6-17和7-28位的Seq ID No 331;4-32,56-61和35-52位的Seq ID No 332;4-14,27-71,74-88,93-110,115-120,124-130,139-154,161-172和146-171位的Seq ID No 333;4-21和3-15位的SeqID No 334;12-17和9-26位的Seq ID No 335;10-21,45-58和51-67位的Seq ID No 336;59-66,68-84和13-42位的Seq ID No 337;11-16和1-16位的Seq ID No 338;4-19,23-37和10-30位的Seq ID No 339;19-27,35-46,48-66,82-88,99-105,113-119和42-59位的Seq ID No 340;135-147位的Seq ID No 171;658-682位的Seq ID No 172;411-427和1226-1246位的Seq ID No 173;794-817和801-824位的Seq ID No 174;468-492和474-495位的Seq ID No 177;366-388位的Seq ID No 178;266-291,287-312和308-333位的Seq ID No 179;197-213和195-211位的Seq ID No 180;252-275,262-285和812-830位的Seq ID No 181;94-112,97-120和104-128位的Seq ID No 182;257-281位的Seq ID No183;106-134位的Seq ID No 184;70-86位的Seq ID No 187;358-383,378-402,397-421,499-524,520-545,541-566,622-646,641-665和660-684位的Seq ID No 189;248-260位的Seq ID No 192;15-34位的Seq ID No 194;112-129位的Seq ID No 195;333-358和353-378位的Seq ID No 196;316-343,339-366和362-389位的Seq ID No 197;98-123和104-126位的Seq ID No 198;20-43和23-48位的Seq ID No201;124-145位的Seq ID No 203;717-738位的Seq ID No 204;37-56位的Seq ID No 205;118-134位的Seq ID No 206;500-522位的Seq IDNo 208;32-47位的Seq ID No 211;25-51,47-73和69-95位的Seq IDNo 212;503-529位的Seq ID No 215;112-128位的Seq ID No 216;181-199位的Seq ID No 218;109-121位的Seq ID No 219;150-163位的Seq ID No 220;77-97位的Seq ID No 221;564-586位的Seq ID No223;75-94位的Seq ID No 224;776-798,784-808和794-815位的SeqID No 225;196-212,78-100和85-107位的Seq ID No 226;536-553位的Seq ID No 230;102-125位的Seq ID No 232;178-198位的Seq ID No233;612-626位的Seq ID No 238;171-187位的Seq ID No 245;296-320,315-339,334-358和353-377位的Seq ID No 246;47-71位的Seq ID No247;1-25,20-45和40-64位的Seq ID No 248;146-161位的Seq ID No249;910-935和1007-1030位的Seq ID No 251;212-226位的Seq ID No252;126-152,148-173和169-195位的Seq ID No 253;288-310和293-316位的Seq ID No 255;293-312位的Seq ID No 257;154-170位的Seq ID No 258;72-95,90-112和97-121位的Seq ID No 259;135-150和146-163位的Seq ID No 262;799-827位的Seq ID No 263;23-43和33-53位的Seq ID No 266;44-62位的Seq ID No 268;6-22位的Seq IDNo 276;37-54位的Seq ID No 280;40-54位的Seq ID No 281;7-21位的Seq ID No 282;4-11,16-34,48-55,67-77,87-106和153-183位的SeqID No 425;22-40,49-65,70-91,95-109,111-125,146-207,209-216,219-225,229-244,251-270,274-286,292-309,316-329,335-355,358-370,376-388,392-419,425-430,435-441,448-455,464-478,486-515和437-465位的Seq ID No 426;5-19,25-31,43-48,60-79,88-100,105-129,148-171,187-193,243-263,316-322,334-340,345-351,369-378,381-391,399-404,474-483,502-517,525-530,558-568,579-596,622-627,631-638,644-651,653-660,674-680,687-693,721-728,743-753,760-775,788-795,806-813,821-828,835-842,847-859,868-887和300-347位的Seq ID No 427;5-26,37-44,89-97,112-118,121-128,138-154,157-165,176-181,188-198,205-218,223-243,247-253,260-279和76-155位的Seq ID No 428;4-29,41-46,49-68,82-88,121-147,158-164,187-193,195-208,229-236,244-249,251-263,269-275,307-313,337-343,348-381,392-398,402-408,432-438,85-117和194-239位的Seq ID No 429;5-12,14-22,28-34,40-46,70-79,84-129,152-165,174-182和37-109位的Seq ID No 430;5-16,18-52,54-72,81-86,118-126,136-145,151-157,168-180,209-233,244-270,295-302,315-326,329-337,345-352,364-373,397-402,408-418,424-431,435-443,472-480,483-489,504-510,519-527,549-564,576-599,605-637,641-673和91-98位的Seq ID No 431;23-36,42-52,133-140,151-157,242-247,267-277,295-301,320-328,333-339,345-352,365-371,397-403,415-428,456-465,481-487,489-495,508-516,518-527,585-592,606-614,631-637,643-658,665-670,723-728,737-744,752-759,787-793,835-841,873-885,918-928,938-945,951-966,978-988,1015-1020,1030-1036,1044-1052,1058-1069,1071-1079,1081-1088,1113-1119,1125-1138,1141-1147,1164-1170,1172-1177,1190-1200,1214-1220,1230-1236,1239-1245,1262-1268,1270-1275,1288-1298,1312-1318,1328-1334,1337-1343,1360-1366,1368-1373,1386-1396,1410-1416,1426-1432,1435-1441,1458-1464,1466-1471,1484-1494,1508-1514,1524-1530,1533-1539,1556-1562和307-340位的Seq ID No 432;19-25,35-41,44-50,66-72,74-79,92-102,116-122,132-138,141-147,164-170,172-177,190-200,214-220,230-236,239-245,262-268,270-275,288-298,312-318,328-334,337-343,360-366,368-373,386-396,410-416,426-432,435-441,458-464,466-478,504-524,79-148,177-246,275-344和373-442位的Seq ID No 433;7-14,16-23,33-39,46-53,72-79,92-115,123-130,156-175,179-187,214-220,239-246,266-274,302-325,338-354,360-370,375-390,392-401,421-428,430-463和29-58位的SeqID No 434;4-9,22-39,58-65,72-82,87-92,99-104,107-119,143-166,171-177,194-202,205-213,220-228,231-240,247-263,309-315,317-323,336-343和294-320位的Seq ID No 435;4-10,12-18,24-29,34-43,50-65,70-76,111-117,129-138,152-159,166-171,184-195,200-210,224-236,241-251,274-283,285-296,313-319,332-341,348-355,378-386,410-416,433-445,475-482,523-529,531-540,584-596,626-633,674-680,682-688,738-750,780-787,828-834,836-842,853-862,882-887,893-912和604-676位的Seq ID No 436;15-38,49-57,60-99,103-119,124-194,200-206,215-249,251-291,307-313,315-347,369-378,383-390,393-400,405-411,423-435,440-447,454-460,470-486,490-503,532-539,542-549,551-567,579-592和509-583位的Seq ID No 437;38-44,47-88,95-103,157-172,235-240,250-260,263-276,294-300,312-317,331-337,369-391,412-419,442-448,453-463,490-529,537-555,571-580,600-617,619-627,642-648,682-687,693-700,716-722,738-748,756-763,779-789,796-802,820-828,833-840,846-853,862-872,880-887,894-899,924-937,957-963,1006-1012,1043-1049,1063-1069,1076-1097和124-147位的Seq ID No 438;4-28,31-49,60-71,75-102,104-114,134-144,160-184,250-257,277-285,287-294,330-338,345-351,367-374,381-388,393-399,402-407,420-426,443-448,458-464,411-436和454-488位的Seq ID No 439;20-27,45-55,57-64,66-77,98-106,130-137,155-165,167-174,176-187,194-203,208-223,227-238,245-251,257-270,273-278,287-299,330-345,352-358,363-385,392-399,410-417,437-443,467-484,486-492,495-500,504-516,526-536和219-270位的Seq ID No 440;11-22,24-31,46-63,65-71,73-88,95-109,174-181,183-201,204-212,216-222,228-233,241-247和142-221位的Seq ID No 441;8-28,51-59,67-84,93-98,140-152,154-162,183-188和91-125位的Seq ID No 442;10-22,27-61和69-100位的Seq ID No 443;7-15,18-26,94-100,126-131,152-165,219-228,254-263,274-292,297-308,333-340,342-352,354-371,373-379,403-410,420-438,450-456,463-470,489-495,503-512和97-173位的Seq ID No 444;4-21,37-43,49-65,67-74,76-90,113-119,131-141,155-173,175-189,192-199,207-221,247-254,266-276,317-322,337-343,387-393,408-428,439-448,451-460,469-479,482-487,493-501,517-523,533-542和480-503位的Seq ID N0445;11-26,40-46,78-86,93-103,121-126,132-138,166-177,183-196,203-212,214-221,228-263,304-311,323-338,345-351,357-363,379-393,420-434,442-448,518-527,547-553,581-591,602-609,637-645,665-674,687-692,701-708,730-739,796-802,844-857,882-888,903-914,944-950,976-983,1027-1033,1049-1057,1066-1072,1085-1092,1120-1127,1137-1144,1153-1158,1165-1176,1181-1187,1221-1230,1238-1244,1269-1274和605-632位的Seq ID No 446;6-47,57-65,83-95,109-121,138-147,154-164,167-177,194-200,202-212,227-234,240-253,260-267,283-291,320-329,340-347,356-364,412-422,430-436,441-459,465-475,478-486,498-507和59-84位的SeqID No 447;10-21,58-83,88-97,120-126和21-51位的Seq ID No 448;5-39,56-62,76-88,90-114,138-162,170-195,202-221,228-250,264-270,304-355,374-387,391-416,462-471,526-546,554-561,574-579,639-645,651-660,674-682,689-694和666-697位的Seq ID No 449;6-30,36-42,143-157,176-197,202-209,216-233,241-246,275-287,292-299,315-325,343-350,375-380,397-403,411-420,422-434,441-448,467-474,477-499,555-568,591-597,601-609,623-644,667-688,692-698,703-718,736-747,757-766,782-791,795-801,832-840,859-865和226-269位的Seq ID No 450;6-23,43-51,61-67,73-82,91-97,123-130,149-158,164-175,228-234,240-246,248-255,262-272,326-332,340-347,365-371,377-388,409-419,425-431,438-445,449-457,464-470,496-507,559-568,575-581,603-608,617-623,633-639,648-654,659-670,695-701,734-752,806-814,816-829,861-868,891-899,904-909,937-945,947-960,978-983,992-999,1022-1031,1068-1076,1078-1091,1109-1114,1123-1130,1153-1162,1199-1207,1209-1222,1254-1261,1284-1293,1330-1338,1340-1353,1371-1376,1385-1392,1415-1421,1433-1438,1460-1465,1470-1492和1422-1458位的Seq ID No 451;82-94,111-118,125-131,206-212,261-266,310-320,328-338,345-351,353-360,414-420,424-434,440-447,451-500,506-516,548-561,566-572,584-591,601-622,630-636,650-659,661-667,674-699,703-711,717-729,736-744,752-759,765-771,813-822,826-842,852-868,870-877,881-895,897-906,913-922和602-671位的Seq ID No 452;12-18,20-25,43-54,56-65,73-79,82-88,99-111,136-142,153-169,171-183,195-223,229-248,255-260,272-277,281-292,298-319,322-329,332-351,363-379,381-389和275-304位的Seq ID No 453;4-9,34-48,65-77,101-106,111-131,138-153,186-191,230-250和148-219位的Seq ID No454;4-23,30-35,42-53,67-76,82-87,101-108,112-130,132-138,147-152,161-183,187-208,218-225,265-283,295-303,306-317,322-334,338-357,360-368,370-383,387-398,400-419,421-430,104-182和240-304位的Seq ID No 455;4-12,63-69,94-102,146-164,166-173,175-181,193-207,263-281,286-295,301-306,330-343,369-378,382-388,414-420,422-430,438-454,456-462,472-531,543-560,581-591,596-605,614-623,626-635,656-662,669-676,683-690,693-698,705-711,728-736,752-764和69-102位的Seq ID No456;6-12,43-53,141-147,164-179,185-195,197-206,227-235,237-271,288-305,308-317,335-341,351-357,365-376,386-395,397-416,422-447和11-35位的Seq ID No 457;16-24,50-65,73-84,88-99,114-124,130-146,181-187,193-203,214-220,236-247,250-258,287-297和50-113位的Seq ID No 458;4-25,50-55,76-82,117-123,131-137,139-148,157-166,239-245,253-258,266-275,277-292,300-306,51-83和93-161位的Seq ID No 459;6-22,34-43,51-86,93-100,110-116,150-161,164-171,180-187,197-218和168-237位的Seq ID No460;4-27,55-60,74-82和10-46位的Seq ID No 461;6-19,25-31,43-49,60-79,88-100,105-129,148-161,164-171,187-193,243-263,316-322,334-340,369-378,381-391,398-404,460-466,474-483,502-509,511-517,525-530,558-567,579-596,622-627,631-638,641-651,653-659,674-680,687-693,710-716,720-727,743-753,760-775,788-795,806-813,821-828,836-842,847-860,865-880和258-377位的Seq ID No 462;4-11,25-64,71-79,88-94,107-120,123-132,167-188,231-237,240-246,261-267,306-311,330-342,351-358,389-395,406-418,429-434,439-448,483-501,511-520和71-143位的Seq ID No463;4-18,22-27,53-64,94-100,121-127,133-139,155-164,177-182,187-196,206-218,224-242,248-253,258-277和184-253位的Seq ID No464;10-17,56-67,72-82,94-99,106-113,166-173,229-235,243-283,295-301,313-321,326-331,342-348,396-414,423-435,446-452,454-462,496-502,511-534,543-556,563-570,586-593,616-626,638-645,653-662,679-696,731-737,766-774,776-782,790-796,810-817,825-835,837-846和540-615位的Seq ID No 465;13-24,30-36,73-81,89-95,109-115,117-143,161-173,179-189,226-244,251-261,275-281,298-305,307-315,323-328,364-374,69-186和264-354位的Seq 1D No 466;19-25和6-22位的Seq 1D No 467;6-39,59-68和43-62位的Seq ID No 468;6-14,22-32和1-27位的Seq ID No 469;4-41和28-40位的Seq ID No 470;8-14和4-19位的Seq ID No 471;4-10,12-22,30-35和6-33位的Seq ID No 472;4-16,24-33和37-54位的Seq ID No473;2-23位的Seq ID No 474;4-21,27-33,36-41和14-34位的Seq IDNo 475;4-14,24-30,37-42,57-78,83-89,94-103,113-131和100-122位的Seq ID No 476,以及包含所述序列至少6个优选地多于8个尤其多于10个氨基酸并且优选地不多于70、50、40、20、15或11个氨基酸的片段。所有这些片段单独地并且每一个独立地形成了本发明优选的挑选的方面。
通过一套长度至少10个氨基酸有1个氨基酸重叠的肽分析蛋白质抗原的全部序列可以鉴别特定抗原的所有线性超免疫血清反应片段。随后,通过使用表达的全长蛋白质或其结构域多肽的用超免疫血清分析蛋白质抗原可以鉴别非线性表位。假设蛋白质独特的结构域足够形成不依赖于天然蛋白质的3D结构,各个重组或合成产生的结构域多肽用超免疫血清的分析将允许在多结构域蛋白质各个结构域内的构象抗原结构域的鉴别。对于结构域拥有线性以及构象性表位的那些抗原,用相应于线性表位的肽的竞争试验可用于证实构象性表位的存在。
将理解本发明尤其也涉及编码前面提到的片段的核酸分子,杂交编码片段的核酸分子的核酸分子,特别是在严格条件下杂交的那些,以及用于扩增编码片段的核酸分子的例如PCR引物的核酸分子。在这些点上,优选的核酸分子是如上面讨论的相应于优选片段的那些。
本发明也涉及包含本发明的一个或多个核酸分子的载体、用本发明的载体进行基因工程的宿主细胞以及通过重组技术的超免疫血清反应性抗原及其片段的产生。
大量的表达载体能用于表达根据本发明的超免疫血清反应性抗原及其片段。一般,适于维持、繁殖或表达核酸以在宿主中表达多肽的任何载体可以用于在这方面的表达。与本发明的这个方面一致,载体可以例如是质粒载体、单或双链噬菌体载体、单或双链RNA或DNA病毒载体。此处公开的起始质粒或者是市售可获得的、公开可获得的,或者通过众所周知的公开的程序的常规应用能从可获得的质粒构建。在某些方面,载体之中优选的是为了本发明的核酸分子和超免疫血清反应性抗原或其片段的表达的那些。宿主细胞中的核酸构建体能以传统方式用于产生重组序列编码的基因产物。或者,本发明的超免疫血清反应性抗原及其片段能通过传统肽合成器合成产生。成熟的蛋白质能表达于哺乳细胞、酵母、细菌或其它在合适的启动子控制下的细胞。使用来源于本发明的DNA构建体的RNA,也能采用无细胞的翻译系统以产生这样的蛋白质。
能对宿主细胞进行基因工程化以整合核酸分子和表达本发明的核酸分子。合适宿主的代表性实例包括例如肠球菌、葡萄球菌、大肠杆菌、链霉菌和枯草芽孢杆菌的细菌细胞;例如酵母细胞和曲霉属细胞的真菌细胞;例如果蝇S2和夜蛾Sf9细胞的昆虫细胞;例如CHO,COS,Hela,C127,3T3,BHK,293和Bowes黑素瘤细胞的动物细胞;以及植物细胞。
本发明也提供了产生粪肠球菌超免疫血清反应性抗原及其片段的方法,包括从宿主细胞表达本发明提供的核酸分子编码的超免疫血清反应性抗原或其片段。本发明还提供了产生表达粪肠球菌超免疫血清反应性抗原或其片段的细胞的方法,包括用根据本发明的载体转化或转染合适的宿主细胞使得转化的或转染的细胞表达载体中含有的核酸编码的多肽。
可以以例如融合蛋白质的修饰形式表达多肽,并且多肽可以不仅包括分泌信号还有额外的异源性功能区域。因而,例如,尤其是带电荷氨基酸的额外氨基酸区域可以加到多肽的N或C末端以改进在纯化过程中或随后的操作和储存过程中在宿主细胞中的稳定性和持续性。区域也可加到多肽以促进纯化。这样的区域可以在多肽的最后制备之前去除。多肽基团加到多肽以引起分泌或排泄、以改进稳定性或促进纯化在其它之中是本领域熟悉的和常规的技术。优选的融合蛋白质包含对于溶解或纯化多肽是有用的来自免疫球蛋白的异源性区域。例如,EP-A-O 464 533(加拿大相应申请2045869)公开了包含免疫球蛋白分子的恒定区不同部分和另一个蛋白质或其部分的融合蛋白质。在药物发现中,例如,为了鉴别拮抗剂的高通量筛选试验的目的蛋白质已经与抗体Fc部分融合。见例如{Bennett,D.等人,1995}和{Johanson,K.等人,1995}。
通过众所周知的方法能从重组细胞培养物中重新获得和纯化粪肠球菌超免疫血清反应性抗原或其片段,包括硫酸铵或乙醇沉淀、酸提取、阴离子或阳离子交换色谱、磷酸纤维素色谱、疏水相互作用色谱、羟基磷灰石色谱和凝集素色谱。
通过化学合成以及通过生物工程手段能生产根据本发明的超免疫血清反应性抗原及其片段。后者包含了用含有根据本发明的核酸的载体转染或转化宿主细胞,以及在本领域技术人员已知的条件下培养转染或转化的宿主细胞。为了纯化或分离要生产的多肽的生产方法也可以包含纯化步骤。优选的实施方案中载体是根据本发明的载体。
根据本发明的超免疫血清反应性抗原及其片段可用于含有这些生物体或其衍生的多肽的样品中一个或多个生物体的检测。优选的这样的检测是为了诊断,更加优选地为了疾病的诊断,最优选地为了与革兰氏阳性细菌的存在或丰度相关或相联的疾病的诊断,尤其是选自肠球菌、葡萄糖球菌和乳球菌的细菌。更加优选地,微生物选自无乳链球菌(Streptococcus agalactiae),肺炎链球菌(Streptococcuspneumoniae)和变形链球菌(Streptococcus mutans),尤其微生物是粪肠球菌。
本发明也涉及诊断试验,例如用于检测细胞和组织中本发明的超免疫血清反应性抗原及其片段的水平的定量和诊断试验,包括正常和异常水平的测定。所以,例如,根据本发明,为了检测与正常组织样品相比多肽的过度表达的诊断试验可以用于检测感染的存在,以及鉴别感染生物体。能用于测定源自宿主的样品中多肽水平的试验技术对于本领域技术人员是众所周知的。这样的试验方法包括放射免疫试验、竞争结合试验、Western斑点分析和ELISA试验。这些之中,ELISA常常是优选的。ELISA试验最初包含制备特异于多肽的抗体,尤其是单克隆抗体。另外,一般地制备了结合于单克隆抗体的报告抗体。报告抗体附着于检测试剂,例如放射活性的、荧光的或酶试剂,例如辣根过氧化物酶。
根据本发明的超免疫血清反应性抗原及其片段可用于阵列的目的或与其相关。更加特别的,至少一个根据本发明的超免疫血清反应性抗原及其片段可以固定于支持物上。所述支持物一般包含各种超免疫血清反应性抗原及其片段,由此可以使用一种或一些根据本发明的超免疫血清反应性抗原及其片段和/或不同的超免疫血清反应性抗原及其片段产生多样性。这样的阵列以及任何阵列的特征性特性一般是独特的多肽固定于所述支持物或其表面上独特的或预先定义的区域或位置上。因为这个,阵列独特位置或区域上的任何活性与特异性多肽相关。不同超免疫血清反应性抗原及其片段固定于支持物的数量可以在从10个那么少到几千个不同超免疫血清反应性抗原及其片段的范围内。优选实施方案中每cm2超免疫血清反应性抗原及其片段的密度是从每cm210个肽/多肽那么少到每cm2至少400个不同肽/多肽,更加特别的每cm2至少1000个不同超免疫血清反应性抗原及其片段。
这种阵列的生产对于本领域的技术人员是已知的,例如,在美国专利5,744,309中有描述。阵列优选地包含具有至少第一个表面的平坦的、多孔或非多孔的固相支持物。如此出公开的超免疫血清反应性抗原及其片段固定于所述表面。其它之中优选的支持物材料是玻璃或纤维素。为了此处描述的任何诊断应用使用矩阵也在本发明内。除了根据本发明的超免疫血清反应性抗原及其片段外,为了如上面描述的阵列的产生也可以使用根据本发明的核酸。这个也应用于抗体制成的阵列,优选地其它之中如此处描述的单克隆抗体。
另一方面本发明涉及指向任何根据本发明的超免疫血清反应性抗原及其片段、其衍生物或片段的抗体。本发明包括例如单克隆和多克隆抗体、嵌合、单链和人源化抗体,以及Fab片段或Fab表达文库的产物。抗体可以是嵌合的也在本发明之内,即它们的不同部分源自不同种属或者至少各自的序列取自不同的种属。
通过将超免疫血清反应性抗原及其片段直接注射到动物或者通过给予优选非人类的动物超免疫血清反应性抗原及其片段,能够获得产生的抗相应于本发明的序列的超免疫血清反应性抗原及其片段的抗体。然后如此得到的抗体将结合超免疫血清反应性抗原及其片段本身。以这个方式,甚至仅仅编码超免疫血清反应性抗原及其片段的一个片段的序列能用于产生结合整个天然超免疫血清反应性抗原及其片段的抗体。然后这样的抗体能用于从表达那些超免疫血清反应性抗原及其片段的组织中分离超免疫血清反应性抗原及其片段。
为了单克隆抗体的制备,能够使用本领域已知的提供连续细胞系培养物产生的抗体的任何技术(如原先{Kohler,G.等人,1975}中描述地)。
能使为了单链抗体的产生描述的技术适应于产生抗根据本发明的免疫原性的超免疫血清反应性抗原及其片段的单链抗体。转基因小鼠或例如其它哺乳动物的其它生物体可以用于表达抗根据本发明的免疫原性超免疫血清反应性抗原及其片段的人源化抗体。
或者,噬菌体展示技术或核糖体展示能用于从筛选拥有各自靶抗原的人类淋巴细胞的PCR扩增v-基因文库或从幼稚文库挑选对超免疫血清反应性抗原及其片段有结合活性的抗体基因{McCafferty,J.等人,1990};{Marks,J.等人,1992}。也能通过链改组(chain shuffling){Clackson,T.等人,1991}改进这些抗体的亲和力。
如果存在两个抗原结合结构域,每个结构域可以指向不同的表位-称为“双特异性”抗体。
上面描述的抗体可以应用于分离或鉴别表达超免疫血清反应性抗原及其片段的克隆或为了用亲和色谱的分离和/或纯化通过将抗体附着于固相支持物而纯化本发明的超免疫血清反应性抗原及其片段。
因而,抗本发明的超免疫血清反应性抗原及其片段的抗体尤其可以应用于抑制和/或治疗感染,特别是细菌感染,特别是来自粪肠球菌的感染。
超免疫血清反应性抗原及其片段包括抗原性、表位或免疫原性等同的衍生物,它形成了本发明的特定方面。如此处使用的术语“抗原性等同衍生物”包含某些抗体特异性识别的超免疫血清反应性抗原及其片段或其等同物,所述抗体当针对根据本发明的蛋白质或超免疫血清反应性抗原及其片段培养时,干扰病原体和哺乳动物宿主之间的相互作用。如此处使用的术语“免疫原性等同衍生物”包含肽或其等同物,当其用于合适的制剂以在脊柱动物中培养抗体时,抗体起干扰病原体和哺乳动物宿主之间相互作用的作用。
超免疫血清反应性抗原及其片段,例如抗原性或免疫原性等同衍生物或其融合蛋白能够用作抗原以免疫小鼠或例如大鼠或小鸡的其它动物。融合蛋白质可以给超免疫血清反应性抗原及其片段提供稳定性。抗原可以例如通过结合连接于例如牛血清白蛋白(BSA)或匙孔血蓝蛋白(KLH)的免疫原性载体蛋白质。或者,包含蛋白质或超免疫血清反应性抗原及其片段多个拷贝的抗原肽或抗原性、免疫原性等同的超免疫血清反应性抗原及其片段可以有足够的抗原性以改进免疫原性而避免载体的使用。
优选地,修饰抗体或其衍生物使其在个体有更少的免疫原性。例如,如果个体是人,抗体最优选地可以是“人源化的”,其中杂交瘤衍生的抗体的互补决定区域被接种到人单克隆抗体,例如如{Jones,P.等人,1986}或{Tempest,P.等人,1991}中描述地。
本发明的多核苷酸在基因免疫中的使用将优选地采用合适的递送方法,例如质粒DNA直接注射入肌肉,与特异性蛋白质载体复合的DNA的递送,用磷酸钙的DNA共沉淀,DNA包装在各种形式的脂质体中,粒子轰击{Tang,D.等人,1992},{Eisenbraun,M.等人,1993}以及使用克隆的逆转录病毒载体的体内感染{Seeger,C.等人,1984}。
一方面本发明涉及结合于根据本发明的任何超免疫血清反应性抗原及其片段的肽,以及这样肽的生产方法,由此所述方法特征在于根据本发明的超免疫血清反应性抗原及其片段的使用,并且基础步骤对于本领域的技术人员是已知的。
可以通过使用根据本领域技术的方法产生这样的肽,例如噬菌体展示或核糖体展示。在噬菌体展示的情况下,基本上产生了噬菌体形式的肽的文库,这种文库与靶分子接触,在这个情况下是根据本发明的超免疫血清反应性抗原及其片段。随后从各自的反应中去除那些结合于靶分子的肽,优选地是与靶分子的复合物。结合的特性至少在某个程度依赖于例如盐浓度等等特定实行的实验设置是本领域技术人员已知的。结合于有更高的亲和力或更大力量的靶分子的那些肽从文库的非结合成员分离之后,并且可选地也在靶分子从靶分子与肽的复合物去除后,随后可以特征分析各自的肽。特征分析之前,可选地实行扩增步骤,例如通过增殖编码肽的噬菌体。特征分析优选地包含靶结合肽的测序。基本上,肽不限制它们的长度,然而优选地在各自的方法中优选地得到具有从大约8至20个氨基酸的长度的肽。文库的大小可以是大约102至1018,优选地108至1015个不同的肽,然而并不限于这些。
特定形式的靶点结合超免疫血清反应性抗原及其片段是所谓的“抗促成素”,在其它之中它们在德国专利申请DE 197 42 706中有描述。
在另一方面本发明涉及与任何的根据本发明的超免疫血清反应性抗原及其片段相互作用的功能性核酸,以及这样功能性核酸的生产方法,由此所述方法特征在于根据本发明的超免疫血清反应性抗原及其片段的用途,并且基础步骤对于本领域的技术人员是已知的。功能性核酸优选地是适体和镜面体。
适体是单链或双链的、并且特异性与靶分子相互作用的D-核酸。适体的生产或挑选在例如欧洲专利EP 0 533 838中有描述。基本上实行了下面的步骤。首先,提供了核酸的混合物,即潜在的适体,由此每个核酸一般包含一些优选地至少8个随后随机的核苷酸的片段。这个混合物随后与靶分子接触,由此例如基于与候选混合物对靶点增加的亲和力或对靶点更大的力量核酸与靶分子结合。随后从剩余的混合物中分离结合核酸。可选地,使用例如多聚酶链式反应扩增这样得到的核酸。这些步骤可以重复数次,只要最后混合物具有增加的特异性结合于靶点的核酸的比例,然后可选地从中挑选最终结合核酸。这些特异性结合的核酸指的是适体。很明显在适体的生产或鉴别方法的任何阶段,为了使用标准技术测定它的序列可以采集个体核酸混合物的样品。例如通过引入对产生适体的技术领域技术人员已知的定义的化学基团可以稳定适体在本发明之内。这样的修饰例如可以存在于氨基基团在核苷酸糖基团2’位置的引入。适体目前用作治疗剂。然而,这样挑选的或产生的适体可以用于靶点确认和/或用作优选基于小分子药剂的药剂开发的先导物质也在本发明之内。这个实际上通过竞争试验完成,由此候选药物抑制靶分子和适体之间特异性相互作用,由此在适体从靶点和适体的复合物取代时,可以假设各自的候选药物允许靶点和适体之间相互作用的特异性抑制,如果相互作用是特异性的,所述候选药物将至少在原则上适于阻断靶点并因而降低其在包含这样靶点的各自的系统中的生物利用度或活性。这样得到的小分子可以进行进一步的衍生化和修饰以优化其物理、化学、生物和/或医学特征,例如毒性、特异性、生物降解度和生物利用度。
镜面体和它们的产生或制造基于一个简单的原则。镜面体的制造在国际专利申请WO 98/08856中有描述。镜面体是L-核酸,这是指它们由L-核苷酸而不是象适体的D-核苷酸组成。镜面体特征在于它们在生物系统具有非常高的稳定性并且与适体相比特异性地与它们指向的靶分子相互作用这个事实。产生镜面体的方法中,产生了D-核酸的异源群,这个群与靶分子的光学对映体接触,在这个情况下例如与根据本发明超免疫血清反应性抗原及其片段的天然发生的L-对映体的D-对映体接触。随后,分离不与靶分子的光学对映体相互作用的那些D-核酸。但是分离、可选地鉴别和/或测序与靶分子的光学对映体相互作用的那些D-核酸,随后基于从D-核酸获得的核酸序列信息合成相应的L-核酸。就与靶分子的光学对映体相互作用的前面提到的D-核酸的序列而言相同的这些L-核酸将特异性地与天然发生的靶分子而不是它的光学对映体相互作用。与适体产生的方法相似,重复各种步骤几次并因而浓集特异性与靶分子的光学对映体相互作用的那些核酸是可能的。
在另一方面,本发明涉及与根据本发明的任何核酸分子相互作用的功能性核酸,以及这样功能性核酸的制造方法,由此所述方法特征在于根据本发明的核酸分子和它们各自序列的用途并且基础步骤对于本领域技术人员是已知的。功能性核酸优选地是核酶、反义寡核苷酸和siRNA。
核酶是催化活性的核酸,优选地由基本上包含两个基团的RNA组成。第一个基团显示了催化活性而第二基团负责与靶核酸的特异性相互作用,在这个情况下是编码根据本发明的超免疫血清反应性抗原及其片段的核酸。靶核酸和核酶第二基团相互作用时,一般通过两个杂交链上基本上互补的碱基的延伸杂交和Watson-Crick碱基配对,催化活性基团可以变成有活性的,就是指它或者分子内或者分子间催化核酸分子,倘若核酶的催化活性是磷酸二酯酶活性。随后,可以有靶核酸进一步的降解,最后导致了靶核酸以及源自所述靶核酸的蛋白质的降解。核酶、它们的使用和设计原则对于本领域技术人员是已知的,例如{Doherty,E.等人,2001}和{Lewin,A.等人,2001}中有描述。
用于药剂的制造以及作为诊断试剂的反义寡核苷酸的活性和设计分别基于作用的相同模式。基本上,反义寡核苷酸基于碱基互补与靶RNA优选地与mRNA杂交,因而激活RNase H。RNase H被磷酸二酯和硫代磷酸酯偶联的DNA激活。然而除了硫代磷酸酯偶联的DNA以外,磷酸二酯偶联的DNA很快地被细胞内核酶降解。这些抗性的非天然发生DNA衍生物在与RNA杂交时不抑制RNase H。换而言之,反义聚核苷酸仅仅作为DNA RNA杂交复合物是有效的。这种反义寡核苷酸的实例在其它之中的美国专利US 5,849,902和US 5,989,912。换言之,基于靶分子的核酸序列,在这个情况下靶分子是根据本发明的超免疫血清反应性抗原及其片段的核酸分子,可以基于碱基互补的原则,从原则上可从其推测的各自的核酸序列的靶蛋白质或者通过了解尤其是mRNA这样的核酸序列设计合适的反义寡核苷酸。
特别优选的是具有硫代磷酸酯DNA(3至9个碱基)的短的延伸的反义寡核苷酸。最少3个DNA碱基对于细菌RNase H的激活是必需的,最少5个碱基是哺乳动物RNase H激活必需的。在这些嵌合寡核苷酸中有形成RNase H底物的中心区域,它与由不形成RNase H底物的修饰的核苷酸组成的杂交“臂”侧面相接。可以例如通过2’-氧-甲基或2’-氟修饰嵌合寡核苷酸的杂交臂。替代的方法使用了所述臂中的甲基膦酸酯或氨基磷酸酯连接。本发明的实践中有用的反义寡核苷酸进一步实施方案是对甲氧基寡核苷酸,部分对甲氧基寡脱氧核糖核酸或对甲氧基寡核苷酸。
本发明尤其相关和有用的是如上面提到的两个美国专利更加特别描述的那些反义寡核苷酸。这些寡核苷酸不含有天然发生的5′→3′-连接的核苷酸。更合适的寡核苷酸具有两个类型的核苷酸:激活RNase H的2’-脱氧硫代磷酸酯和不激活RNase H的2’修饰的核苷酸。2’-修饰的核苷酸之间的连接是磷酸二酯、硫代磷酸酯或对乙氧基磷酸二酯。通过连续的RNase H激活区域完成RNase H的激活,所述激活区域含有3和5个之间的2’脱氧硫代磷酸酯核苷酸以激活细菌RNase H以及5和10个之间的2’脱氧硫代磷酸酯核苷酸以激活真核特别是哺乳动物RNase H。通过使5’和3’末端碱基高度核酶抗性以及可选地通过取代3’末端阻断基团完成对于降解的保护。
更加特别地,反义寡核酸包含5’末端和3’末端;从位置11至595′→3′-连接的核苷酸独立地选自2’修饰的磷酸二酯核苷酸和2’修饰的对烷氧基磷酸三酯核苷酸;其中5’末端核苷酸附着于三和十个连续的硫代磷酸酯连接的脱氧核糖核酸之间的RNase H激活区域,其中所述核苷酸3’末端选自反转的脱氧核糖核酸、一至三个硫代磷酸酯2’修饰的核糖核酸的连续延伸、生物素基团和对烷氧基磷酸三酯核苷酸。
也可以使用反义寡核苷酸,其中不是5’末端核苷酸而是上面详述的3’末端核苷酸附着于RNase H激活区域。5’末端也选自特定的基团,而不是所述寡核苷酸的3’末端。
根据本发明的核酸以及超免疫血清反应性抗原及其片段可以用作或用于尤其是疫苗的药物组合物的制造。优选是疫苗的这样的药物组合物,优选地是为了粪肠球菌造成的、相关的或相联的疾病的预防和治疗。在这个范围内本发明的另一方面涉及诱导尤其是哺乳动物的个体的免疫反应的方法,包含用本发明的超免疫血清反应性抗原及其片段或其片段或变异体接种个体,足够生产抗体以保护所述个体免受感染,特别是肠球菌感染并且最特别的肠球菌的感染。
仍然本发明的另一方面,涉及诱导个体免疫反应的方法,包含通过基因治疗或其它,递送核酸功能性编码超免疫血清反应性抗原及其片段或其片段或变异体以体内表达超免疫血清反应性抗原及其片段或其片段或变异体,目的是为了诱导产生抗体或细胞介导的T细胞反应的免疫反应以保护所述个体免受疾病,不管那个疾病是否已经在个体内确定,T细胞可以是产细胞因子的T细胞或细胞毒性T细胞。给予基因的一个方法是通过加速作为颗粒上的涂层或其它的基因进入期望的细胞内。
本发明的另一方面涉及免疫组合物,当被引入能够诱导宿主内免疫反应的宿主内时,它能诱导这样的宿主内的免疫反应,其中所述组合物包含编码和表达本发明的超免疫血清反应性抗原及其片段的抗原的重组DNA。可以治疗性或预防性地使用免疫反应,可以采用抗体免疫或细胞免疫的形式,例如源自CTL或CD4+T细胞的细胞免疫。
本发明的超免疫血清反应性抗原及其片段或其片段可以与本身可能不产生抗体但是能够稳定第一个蛋白质并产生将具有免疫性和保护特性的融合蛋白质的辅蛋白质(co-protein)融合。这个融合的重组蛋白质优选地进一步含有抗原性辅蛋白质,例如谷胱甘肽S转移酶(GST)或β-半乳糖苷酶,溶解蛋白质并促进其产生和纯化的相对大的辅蛋白质。另外,辅蛋白质在提供免疫系统全面刺激的意义上起佐剂的作用。辅蛋白质可以附着于第一个蛋白质的氨基端或羧基端。
本发明也提供的是在粪肠球菌感染的动物模型中在这样的基因免疫实验中使用所述核酸分子或者其特定片段的方法。这样的片段对于鉴别能引发预防性或治疗性免疫反应的蛋白质表位尤其有用。这个方法能允许随后从成功抵抗或清除感染的动物的必备器官制备有特别价值的单克隆抗体以开发尤其是人类的哺乳动物中肠球菌感染的预防试剂或治疗处理。
超免疫血清反应性抗原及其片段可以用作宿主免疫接种的抗原以产生抗细菌入侵的保护的特异性抗体,例如通过阻断细菌对损伤组织的粘附。组织损伤的实例包括例如机械、化学或者通过留置装置的植入造成的皮肤或结缔组织以及粘膜组织的伤口,或者例如口腔、乳腺、尿道或阴道的粘膜的伤口。
本发明也包括包含免疫原性重组蛋白质和合适载体的疫苗制剂。因为蛋白质可以在胃内降解,优选地它通过非胃肠道给予,包括例如皮下、肌肉内、静脉、皮内、鼻内或经皮的给予。适于非胃肠道给予的制剂包括水性和非水性无菌注射溶液,它可以含有赋予制剂与个体的优选是血液的体液等渗的抗氧化剂、缓冲剂、细菌抑制剂和溶质;水性和非水性的无菌悬浮液,它可以包括悬浮剂或增稠剂。制剂可以呈现在单位剂量或多个剂量的容器内,例如密封的安瓿和药瓶,可以储存于冷冻干燥条件下,只需要使用前立即加入无菌液体载体。疫苗制剂也可以包括增强制剂免疫原性的佐剂系统,例如水包油系统和本领域已知的其它系统。剂量将依赖于疫苗的特异性活性并能很容易地通过常规试验测定。
根据另一方面,本发明涉及包含如本发明为了粪肠球菌提供的这样的超免疫血清反应性抗原及其片段的药物组合物。这样的药物组合物可包含一个优选地至少两个或两个以上的抗粪肠球菌的超免疫血清反应性抗原及其片段。可选的,这样的粪肠球菌超免疫血清反应性抗原及其片段在联合药物组合物中也可与抗其它病原体的抗原联合。优选地,所述药物组合物是为了预防或治疗粪肠球菌和/或其它病原体造成的感染的疫苗,疫苗中包括了抗所述病原体的抗原。
根据另一方面,本发明涉及包含编码上面鉴别的对于粪肠球菌的超免疫血清反应性抗原或其片段的核酸分子的药物组合物。这样的药物组合物可以包含一个或多个编码抗粪肠球菌的超免疫血清反应性抗原或其片段的核酸分子。可选地,编码超免疫血清反应性抗原或其片段的这样的粪肠球菌核酸分子在联合药物组合物中也可以与编码抗其它病原体的抗原的核酸分子联合。优选地,所述药物组合物是为了预防或治疗粪肠球菌和/或其它病原体造成的感染的疫苗,疫苗中包括了抗上述病原体的抗原。
药物组合物可含有任何适合的辅助物质,例如缓冲物质、稳定剂或另外的活性成分,尤其是已知与药物组合物和/或疫苗生产有关的成分。
根据本发明的超免疫血清反应性抗原、片段或其编码核酸分子的优选的载体和/或赋形剂是为了进一步刺激对给定的超免疫血清反应性抗原、其片段或其编码核酸分子的免疫反应的免疫刺激化合物。优选地根据本发明的药物制备物中的免疫刺激化合物选自尤其是聚阳离子肽的聚阳离子物质、优选是免疫刺激脱氧核苷酸的免疫刺激核酸分子、明矾、弗氏完全佐剂、弗氏不完全佐剂、尤其是人生长激素的神经活性化合物或其组合。
尤其是疫苗的药物组合物除根据本发明的超免疫血清反应性抗原、其片段和/或其编码核酸分子外还包含有生物或药物活性的其它化合物也在本发明的范围内。优选地,疫苗组合物包含至少一个聚阳离子肽。根据本发明使用的聚阳离子化合物可以是显示了根据WO97/30721的特征作用的任何聚阳离子化合物。优选的聚阳离子化合物选自碱性多肽、有机聚阳离子、碱性聚氨基酸或它们的混合物。这些聚氨基酸应当具有至少4个氨基酸残基的链长度(WO 97/30721)。尤其优选的是象聚赖氨酸、聚精氨酸和在多于8个尤其是多于20个氨基酸残基的范围内含有多于20%尤其多于50%的碱性氨基酸的多肽的物质或者它们的联合。其它优选的聚阳离子和它们的药物组合物在WO97/30721(例如聚乙烯亚胺)和WO 99/38528中有描述。优选地这些多肽含有20和500个之间的氨基酸残基,尤其是30和200个之间的残基。
这些聚阳离子化合物可以化学或重组产生或可以来源于天然的来源。
阳离子(多)肽也可以是抗微生物的,有如{Ganz,T.,1999}中综述的特性。这些(多)肽可以是原核的或动物或植物来源的或者可以化学或重组产生(WO 02/13857)。肽也可以属于防卫素类(WO02/13857)。这样的肽的序列例如能在下面网址的抗微生物序列数据库中找到:
http://www.bbcm.univ.trieste.it/~tossi/pag2.html
这样的宿主防御肽或防御物质也是根据本发明的聚阳离子多聚体的优选形式。一般地,允许作为优选地由APC(包括树突细胞)介导的适应性免疫系统的终末产物激活(或下调)的化合物用作聚阳离子多聚体。
尤其优选用作本发明的聚阳离子物质是cathelicidin衍生的抗微生物肽或其衍生物(国际专利申请WO 02/13857,此处引用作为参考),尤其是源于哺乳动物cathelicidin的抗微生物肽,优选地来自人、牛或小鼠。
源于天然来源的聚阳离子化合物包括HIV-REV或HIV-TAT(衍生的阳离子肽、触角足肽、壳聚糖或甲壳素其它的衍生物)或源于生化或重组产生的这些肽或蛋白质的其它肽。其它优选的聚阳离子化合物是cathelin或来自cathelin的相关或衍生的物质。例如,小鼠cathelin是具有氨基酸序列NH2-RLAGLLRKGGEKIGEKLKKIGOKIKNFFQKLVPQPE-COOH的肽。相关的或衍生的cathelin物质含有有至少15-20个氨基酸残基的cathelin序列的全部或部分。衍生化可以包括天然氨基酸被不在20个标准氨基酸中的氨基酸替代或修饰。另外,更多的阳离子残基可以引入这样的cathelin分子中。这些cathelin分子优选地与抗原联合。结果这些cathelin分子作为没有更多的佐剂加入的抗原的佐剂惊人地有效。所以这样的cathelin分子用作有或没有更多的免疫激活物质的疫苗制剂的有效佐剂是可能的。
根据本发明使用的另一个优选的聚阳离子物质是含有被3至7个疏水氨基酸的连接子分开的至少2个KLK基团的合成肽(国际专利申请WO 02/32451,此处引入作为参考)。
本发明的药物组合物可进一步包含免疫刺激核酸。免疫刺激核酸是例如含有中性或人工CpG的核酸,源自非脊柱动物或在某些碱基内容含有非甲基化的胞核嘧啶-鸟嘌呤二核苷酸(CpG)的短寡核苷酸形式(ODN)(例如WO 96/02555中描述的)的核酸的短延伸。或者,基于如例如WO 01/93903中描述的次黄嘌呤核苷和胞啶的核酸或含有脱氧次黄嘌呤核苷和/或脱氧尿苷残基的脱氧核酸(WO 01/93905和PCT/EP 02/05448中描述的,此处引用作为参考)可优选地用作本发明的免疫刺激核酸。优选地,根据本发明也可以使用不同免疫刺激核酸的混合物。
任何上面提到的聚阳离子化合物与如前面提到的任何免疫刺激核酸联合也在本发明之内。优选地,这样的联合是根据WO 01/93905,WO02/32451,WO 01/54720,WO 01/93903,WO 02/13857和PCT/EP02/05448和奥地利专利申请A 1924/2001中描述的那些,此处引用作为参考。
另外或可替代地,这样的疫苗组合物除了根据本发明的超免疫血清反应性抗原及其片段和其编码核酸外还可以包含神经活性化合物。优选地,神经活性化合物是如例如WO 01/24822中描述的人生长因子。也优选地,神经活性化合物联合于如前面提到的任何聚阳离子化合物和/或免疫刺激核酸。
在另一方面,本发明涉及药物组合物。例如这样的药物组合物是此处描述的疫苗。药物组合物也是包含任何下面的化合物或其联合的药物组合物:根据本发明的核酸分子,根据本发明的超免疫血清反应性抗原及其片段,根据本发明的载体,根据本发明的细胞,根据本发明的抗体,根据本发明的功能性核酸和根据本发明的例如抗促成素的结合肽,如此处描述的任何激动剂和拮抗剂。与此有关任何这些化合物可联合于非无菌或无菌载体或与细胞、组织或生物体一起使用的载体而应用,例如适于给予研究对象的药物载体。这样的组合物包含例如介质添加剂或治疗有效量的本发明的超免疫血清反应性抗原及其片段以及药物可接受载体或赋形剂。这样的载体可以包括但不限于生理盐水、缓冲盐、葡萄糖、水、甘油、乙醇和它们的联合。制剂应当适合给药的模式。
药物组合物可以以任何有效的、方便的方式给予,包括例如通过其它之中局部、口腔、肛门、阴道、静脉内、腹腔内、肌肉内、皮下、鼻内、气管内或皮内途径的给予。
在治疗中或作为预防剂,活性试剂可以作为例如无菌水性分散物优选等渗的可注射组合物给予个体。
或者组合物可制成适于局部应用的例如软膏、乳膏、洗液、眼药膏、滴眼液、滴耳液、漱口水、浸透敷料和缝线和烟雾剂的形式,并且可以含有合适的传统添加剂,包括例如防腐剂、辅助药物穿透的溶剂以及药膏和乳膏中的软化剂。这样的局部制剂可以含有相容性传统载体,例如乳膏或软膏基质以及洗剂的乙醇或油醇。这样的载体可以构成大从约1%至大约98%的制剂重量;更加通常地它们将构成一共大约80%的制剂重量。
除了上面描述的治疗外,本发明的组合物一般可用作伤口治疗剂以阻止细菌粘附到受伤组织中暴露的基质蛋白质以及为了牙科治疗的预防用途用作抗生物预防的代替或与抗生物预防结合。
疫苗组合物很方便地是可注射的形式。可采用传统佐剂以增强免疫反应。疫苗接种合适的单位剂量是0.05-5μg抗原/每公斤体重,优选地给予这样的剂量1-3次,间歇1-3周。
在所示剂量范围,本发明的化合物应当没有观察到妨碍它们给予合适的个体副毒性作用。
进一步的实施方案中,本发明涉及诊断的和药物的包装和试剂盒,包含一个或多个充填了一个或多个本发明前面提到的成分的容器。成分能以有用的量、剂量、制剂或联合存在。与这样的容器有关的是管理药物或生物产品的制造、使用或销售的政府机构规定的形式的通知,反映了人类给予的产品的制造、使用或销售的机构的批准。
与本发明相关,如此处公开的例如药物组合物或疫苗的用途相关的任何疾病尤其是肠球菌更加优选地类肠球菌相连或相联的疾病或疾病状态。与此相关,要注意粪肠球菌包含了包括此处公开的那些的一些菌株。与根据本发明要预防和/或治疗的细菌感染相关的、造成的或相联的疾病在人类包括,除了其它的,细菌性咽炎、猩红热、脓包性皮炎、风湿热、坏死性筋膜炎和脓毒症。
仍然进一步的实施方案中,本发明涉及使用任何根据本发明的超免疫血清反应性抗原及其片段的筛选方法。这样的筛选方法对本领域的技术人员是已知的,能设计所述方法以筛选激动剂或拮抗剂。优选地筛选了在本情况下抑制或阻止根据本发明的超免疫血清反应性抗原及其片段与相互作用伴侣结合的拮抗剂。这样的相互作用伴侣可以是天然发生的相互作用伴侣或非天然发生的相互作用伴侣。
本发明也提供了筛选化合物的方法以鉴别增强(激动剂)或阻断(拮抗剂)本发明的超免疫血清反应性抗原及其片段或核酸分子的功能的那些化合物,例如其与结合分子的相互作用。筛选的方法可以包括高通量。
例如,为了筛选激动剂或拮抗剂,可以从表达结合于本发明的超免疫血清反应性抗原及其片段的分子的细胞分别制备根据本发明的核酸分子的相互作用伴侣和核酸,可以是合成反应混合物、细胞腔室,例如膜、细胞被膜或细胞壁或者任何的它们的制备物。制备物在有或无可以是激动剂或拮抗剂的候选分子的存在下与标记的超免疫血清反应性抗原及其片段一起孵育。候选分子与结合分子结合的能力反映在标记配体结合的降低。无偿结合,即不引起超免疫血清反应性抗原及其片段功能性作用的分子最可能是好的拮抗剂。很好结合的并引起与超免疫血清反应性抗原及其片段相同的或密切相关的功能性作用的分子是好的激动剂。
可以例如通过在候选分子与细胞或合适的细胞制备物相互作用后测定报告系统的活性,以及与本发明的超免疫血清反应性抗原及其片段或引发与超免疫血清反应性抗原及其片段相同作用的分子的作用比较测量可能的激动剂和拮抗剂的功能性作用。在这点上有用的报告系统包括但不限于比色标记底物转化为产物,对超免疫血清反应性抗原及其片段的功能性活性的改变有响应的报告基因,以及本领域已知的结合试验。
拮抗剂试验的另一个实例是竞争试验,在竞争抑制试验合适的条件下联合本发明的超免疫血清反应性抗原及其片段和有膜结合的结合分子、重组结合分子、天然底物或配体、底物或配体模仿物的可能的拮抗剂。能例如用放射活性或比色化合物标记超免疫血清反应性抗原及其片段,如此能精确地测定结合于结合分子或转化为产物的超免疫血清反应性抗原及其片段以评价可能的拮抗剂的有效性。
可能的拮抗剂包括结合于本发明的超免疫血清反应性抗原及其片段并因而抑制或取消其活性的的小有机分子、肽、多肽和抗体。可能的拮抗剂也可以是小有机分子、肽、多肽,例如结合于结合分子上相同位点但不引起本发明超免疫血清反应性抗原及其片段的功能活性的密切相关的蛋白质或抗体。
可能的拮抗剂包括小分子,它结合于并占据了超免疫血清反应性抗原及其片段的结合位点,由此阻止与细胞结合分子的结合,使得正常的生物活性被阻止。小分子的实例包括但不限于小有机分子、肽或肽样分子。
其它可能的拮抗剂包括反义分子(对于这些分子的描述,见{Okano,H.等人,1991};寡核苷酸作为基因表达的反义抑制剂;CRCPress,Boca Ration,FL(1988))。
优选的可能的拮抗剂包括本发明的超免疫血清反应性抗原及其片段的衍生物。
如此处使用的根据本发明的超免疫血清反应性抗原及其片段的活性是其结合于任何其相互作用伴侣的能力或结合于其或任何相互作用伴侣这样的能力的程度。
在特定的方面,本发明提供了本发明的超免疫血清反应性抗原及其片段、核酸分子或的抑制剂干扰病原体和负责感染后遗症的哺乳动物宿主之间最初的物理相互作用的用途。尤其是可以在如下方面使用本发明的分子:i)阻止粪肠球菌粘附于植入装置上的哺乳动物细胞外基质蛋白质或伤口的细胞外基质蛋白质;ii)阻断蛋白质介导的哺乳动物细胞侵入,通过例如引发哺乳动物酪氨酸激酶的磷酸化(Rosenshine,I.等人,1992),iii)阻断哺乳动物细胞外基质蛋白质与介导组织损伤的细菌蛋白质之间的细菌性粘附;iv)阻断除了装置的植入或其它手术技术以外例如通过抑制营养的获得启动的感染中致病的正常进程。
此处提供的每个DNA编码序列可以用于抗菌化合物的发现和开发。编码蛋白质在表达时能用作抗菌药筛选的靶点。另外,编码编码的蛋白质氨基酸区域或Shine-Delgarno或各自mRNA的其它翻译促进序列的DNA序列能用于构建反义序列以控制目的编码序列的表达。
可以采用拮抗剂和激动剂以例如抑制源自肠球菌尤其是粪肠球菌感染的疾病,例如脓毒症。
在仍然另一方面,本发明涉及亲和装置,这样的亲和装置包含至少一种支持材料和附着于支持材料的任何根据本发明的超免疫血清反应性抗原及其片段。由于根据本发明的超免疫血清反应性抗原及其片段对它们的靶细胞或靶分子或它们的相互作用伴侣的特异性,只要满足结合的条件,超免疫血清反应性抗原及其片段允许它们的相互作用伴侣从应用于支持材料的任何种类的样品中选择性去除。样品可以是生物或医学样品,包括但不限于发酵肉汤、细胞碎片、细胞制备物、组织制备物、器官制备物、血液、尿液、淋巴液、液体等等。
超免疫血清反应性抗原及其片段可以以共价或非共价方式附着于基质。合适的支持材料对于本领域技术人员是已知的,并选自纤维素、氧化硅、玻璃、铝、顺磁珠、淀粉和右旋糖苷。
本发明进一步通过下面的图示、实例和序列列表举例说明,从中可以取得进一步的特性、实施方案和优点。要理解只经由例证不经由公开的限制给予本实例。
与本发明相关
图1显示了粪肠球菌特异性人血清的特征。
图2显示了来自粪肠球菌V583的小片段基因组文库LEF-70的特征。
图3显示了使用生物素化人IgG通过MACS对细菌细胞的挑选。
表1显示了用粪肠球菌基因组文库和人血清进行的所有筛选的概述,以及通过同源性检索鉴别的粪肠球菌蛋白质。
表2显示了人血清的表位血清学。
在详述中提到的图示在下面有更详细地描述。
图1显示了如免疫试验测量的抗粪肠球菌抗体的人血清的特征分析。使用从粪肠球菌菌株V583制备的总细菌裂解物或培养上清组分,通过标准ELISA测量总抗粪肠球菌IgG抗体水平。(A)显示了健康成人血清的代表性实验结果。数据表示为线性检测范围内的血清稀释度(2.000X和10,000X)在405nm处的吸光度计算来的ELISA单位。(B)为了确保对蛋白质抗原的多重免疫反应性,对通过ELISA挑选的高滴度血清进行免疫斑点分析。显示了使用总细菌裂解物和在5.000X稀释度的挑选的人血清的代表性实验结果。用抗人IgG次级抗体试剂显色斑点。泳道1-4:包括在筛选库中的个体高滴度血清(N1-4),泳道5:低滴度血清(N5),泳道6:阴性对照,(无血清,仅有次级抗体)。MW:分子量标记。(C)显示了来自健康成人(N,编号与B相同)与肠球菌感染(如创伤感染,菌血症,感染的导管)急性期患者(P)的血清中获得的IgG滴度的比较。数据表示为ELISA单位,如对(A)所述计算。(D)显示来自心内膜炎患者的恢复期血清的实验结果。数据表示为线性检测范围内的血清稀释度(2.000X)在405nm处的吸光度计算来的ELISA单位。(E)为了用蛋白质抗原确定多种免疫反应性,用来自心内膜炎的患者之高滴度血清进行免疫斑点分析。显示了在5.000X稀释度使用总细菌裂解物和所选人血清的代表性结果。用抗人IgA次级抗体试剂显示斑点。泳道1-6:来自心内膜炎患者的个体血清。Mw:分子量标记。
图2(A)显示了粪肠球菌V583小片段基因文库LEF-70的片段大小的分布。在测序576个随机挑选的克隆后,修齐序列以消除载体残基,对有各种基因组片段大小的克隆数量作图。(B)显示了粪肠球菌V583染色体之上LEF-70的483个随机测序的克隆分布图。长方形指出了与有注解的ORF配对的序列,菱形代表了与+/+或+/-方向的非编码染色体序列完全匹配的克隆。环形决定了有嵌合序列的所有克隆的位置。碱基对中的数字距离指出了环形基因组上的方向。图底下的数字和百分比给出了文库内各种克隆集合的划分。
图3(A)显示了用生物素化的人IgG的MACS挑选。用20μg生物素化的人血清(IC10-IgG)在第一和第二轮中筛选了pMAL9.1中的LEF-70文库。作为阴性对照,没有血清加入到筛选的文库细胞。显示了对于第一轮的第一次和第二次洗脱之后挑选的细胞数以及第二轮的第一到第4次洗脱后挑选的细胞数,带有或不带有添加的IgG。图3B显示了如用人血清(IC10-IgG,约4μg/μl)的Western印迹分析的2轮细菌表面展示后分离的特异性克隆(1-26个)的反应性,用于通过MACS的挑选在1∶3,000稀释度使用。作为负载对照,相同的斑点用针对平台蛋白质LamB的抗体在高免疫兔血清1∶5,000稀释度也进行分析。LB,来自表达LamB而没有外源肽插入的克隆的提取物。
表1:细菌表面展示鉴别的免疫原性蛋白质。
(A)用高抗肠球菌滴度的来自健康成人的血清鉴别的抗原。A,用IC10-IgG在fhuA中来自粪肠球菌V583的LEF300文库(723),B,在fhuA中的LEF300文库(389),C,在fhuA中的带有IC10-IgG的LEF300文库(1096),D,在fhuA中的带有IC9-IgG的LEF300文库(1065)。表1b显示通过与表1a中所列出的粪肠球菌抗原至少70%同一性的同源性检索鉴别的粪肠球菌蛋白质。用计算机程序TBLASTN鉴别粪肠球菌与屎肠球菌序列之间的同一性(
http://www.hgsc.bcm.tmc.edu/microbial/microbialblast.cgi?organism =Efaecium)。基于相应的粪肠球菌抗原的完整ORF序列计算氨基酸序列的同一性。表1c显示了利用带有高抗肠球菌滴度血清的恢复的心内膜炎患者血清所鉴别的抗原。E,用P25-IgG在fhuA中来自粪肠球菌V583的LEF70文库(843);F,用P26-IgG在fhuA中来自粪肠球菌V583的LEF70文库(845);G,用P25-IgG在fhuA中的LEF300文库(691);H,用P26-IgG在fhuA中的LEF300文库(770);*,程序ANTIGENIC(Kolasker,A,等人,1990)进行长于5个氨基酸的抗原性序列的预测。
表2:用人血清的表位血清学。
显示了代表所选表位的个体合成肽与人血清的免疫反应性。反应性的程度用代码的颜色显示,白色:-(<100U),浅灰:+(100-249U),深灰++(250-349U),黑+++(>350U)。ELISADANW(U)从OD405nm读数以及血清稀释度计算。以所有反应性的加和计算得分(-=0;+=1;++=2;+++=3)。N1-N10血清为来自健康成人的高滴度血清,用于IC9-和IC10-IgG库的筛选。P1-P11为来自患有粪肠球菌感染的患者的血清。给出了根据V583菌株的基因组标注的抗原性ORF中合成肽的位置,显示第一个和最后一个氨基酸残基。肽名称:EF0020.1,存在于染色体上注释的ORF EF0020;ARF0679.1,在替代的开放读框EF0679中的可能的新ORF,EFC0034.1,存在于质粒上的注释的ORF;ARFC0021.1,存在于来自质粒C的替代的开放读框ARFC0021中的可能的新ORF。
实施例
实施例1:基于抗粪肠球菌抗体的人血清来源的表征和挑选、抗体筛选试剂的制备
试验过程
酶联免疫试验(ELISA)。
用包被缓冲液(0.1M碳酸钠pH 9.2)稀释的5-10μg/ml总蛋白质包被ELISA平板(Maxisorb,Millipore)。PBS-BSA中制成三个稀释度的血清。根据生产厂商的推荐(稀释度:1,000x)使用高特异性辣根过氧化物酶(HRP)-连接的抗人IgG或抗人IgA二抗(SouthernBiotech)。通过基于自动ELISA阅读仪(TECAN SUNRISE)OD405nm的读数测量底物转化为颜色产物,定量抗原抗体复合物。
细菌抗原提取物的制备
总细菌裂解物:细菌在BHI(脑-心浸出液)中生长过夜,重复冷冻-解冻循环裂解细菌:在干冰/乙醇混合物上孵育直到冻结(1分钟),然后在37℃解冻(5分钟):重复3次。这之后是超声和离心收集上清(3,500rpm,15分钟,4℃)。
培养上清液:离心去除细菌后,通过混合1份上清和3份无水乙醇,用冰冷的乙醇沉淀过夜生长的细菌培养物的上清,在-20℃孵育过夜。离心收集沉淀物(2,600g,15分钟)。干燥的粒状沉淀物为了ELISA溶于PBS,或为了SDS-PAGE和免疫斑点溶于尿素和SDS样品缓冲液。Bradford试验测定样品的蛋白质浓度。
免疫斑点
从体外生长的粪肠球菌V583株制备总细菌裂解物和培养上清样品。使用BioRad Mini-Protean细胞电泳系统通过SDS-PAGE分离10至25μg总蛋白质/泳道,蛋白质转移到硝酸纤维素膜(ECL,AmershamPharmacia)。在5%的奶中封闭过夜后,加入2,000x稀释的人血清,HRPO标记的抗人IgG用于检测。
用于基因组筛选的抗体的纯化
基于筛选程序中使用的血清合并物的总抗肠球菌滴度挑选来自患者和健康组的五份血清。通过将热灭活血清与全细胞大肠杆菌细胞(DH5α,用pHIE11转化,在与细菌表面展示使用的相同的条件下生长)孵育去除抗大肠杆菌蛋白质的抗体。根据厂商说明书(超连接固定蛋白质G,Pierce)用蛋白质G亲和色谱从合并的耗贫(depleted)血清生产IgG高度富集的制备物。用SDS-PAGE、Western斑点、ELISA和蛋白质浓度测量检查耗贫(depletion)和纯化的效率。
结果
人免疫系统产生的并存在于人血清的抗粪肠球菌抗体是抗原蛋白质的体内表达和它们的免疫原性的指征。这些分子对于如本发明描述的方法中各体抗原的鉴别是必须的,所述方法基于特异性抗肠球菌抗体和相应的肠球菌肽或蛋白质的相互作用。为了获得相关抗体所有组分(repertoires),从健康成人、以及带有肠球菌感染的患者和幼稚个体(5-10月龄的年幼儿童,在其已经丧失母体抗体后)收集人血清。
暴露于病原体的个体中诱导的血清和例如粘膜分泌物的其它体液中的抗体对于抗原鉴别是至关紧要的。暴露于肠球菌导致了无症状的群集、当前或过去的急性或慢性感染。粪肠球菌的群集和感染是普遍的,作为来自以前的遭遇的天然免疫的后果抗体是存在的。由于肠球菌是机会(不是强制性的)致病菌,很可能来自健康个体的血清也含有相关抗体。
通过一系列免疫试验对来自健康成人的70个血清和来自由粪肠球菌引起的心内膜炎的恢复的患者的16份和来自急性感染(主要为创伤和菌血症)的8份患者血清进行抗粪肠球菌抗体的特征分析。使用例如总细菌提取物和从粪肠球菌V583株制备的培养上清蛋白质的两个不同的抗原制备物通过ELISA完成初步特征分析。图1中显示了使用来自健康成人群(图1A)和来自心内膜炎患者(图1D)的血清的典型实验。在反应是线性的给定的稀释度比较抗体滴度。基于IgG的针对两个复合抗原混合物的反应性对血清进行分级,挑选最高的血清进行进一步的免疫斑点测试。这个分析证实预先挑选的血清抗多重肠球菌蛋白质的高抗体反应性(图1B&E),尤其与未挑选的低滴度血清比较时。然而,血清的ELISA分级不总是与免疫斑点信号相关,提示抗非蛋白质组分(例如:脂磷壁质,肽聚糖等)抗体也对针对总细菌提取物的ELISA反应性有贡献。因而,包括在抗体合并物中的血清的最终挑选主要基于免疫斑点试验中的多个免疫原性条带。这个广泛的抗体特征分析方法导致了抗肠球菌超免疫血清的明确的鉴别。这些选自健康成人的血清与来自具有多种急性肠球菌感染(例如,创伤感染、菌血症和导管相关的感染)的患者的血清相比较。显然,抗粪肠球菌抗体滴度在急性患者组较低,提示疾病可在低滴度个体中发生(图1C)。这些患者血清然后主要用于有效性目的,即测量针对所鉴别的表位的抗体水平。但是,患有由粪肠球菌引起的心内膜炎的患者(通过常规微生物诊断证实)在恢复期出现高水平的抗体,如在此处分析的16个样品中所证实的高滴度血清百分比(图1E&D)。
从健康成人中选择10份血清,从患有心内膜炎的恢复患者的供体组选择10份血清。合并血清(每组5份样品),经纯化用于通过细菌表面展示IgG抗体鉴别抗原的IgG从合并的血清中纯化,方法是通过亲和色谱,并耗尽大肠杆菌反应性抗体以避免细菌表面展示筛选中的背景。
实施例2:粪肠球菌的高度随机的、挑选框架的、小片段的基因组DNA文库的产生
实验过程
肠球菌基因组DNA的制备。来自冷冻穿刺培养物的粪肠球菌V583细菌接种于50ml脑心浸出液(BHI)并且在通风和37℃摇晃18h而生长。然后收集培养物,1,600xg离心15分钟,去除上清。用PBS清洗细菌小球3次,小心悬浮于0.5ml的溶菌酶溶液(100mg/ml)。加入0.1ml的10mg/ml热处理的RNase A和20U的RNase T1,小心混合,溶液在37℃孵育1h。0.2ml的20%SDS溶液和0.1ml蛋白酶K(10mg/ml)的加入之后,试管在55℃孵育过夜。然后加入1/3体积的饱和NaCl,溶液在4℃孵育20分钟。微离心机中沉淀提取物(13,000rpm),上清转入新的试管。用PhOH/CHCl3/IAA(25∶24∶1)和用CHCl3/IAA(24∶1)萃取溶液。加入0.6x体积的异丙醇在室温下沉淀DNA,用无菌Pasteur移液管从溶液缠绕DNA并转入含有80%冰冷乙醇的试管中。10-12,000xg离心沉淀物重新获得DNA,然后空气中干燥并溶于ddH2O中。
小基因组DNA片段的制备。使用杯状管(cup-horn)超声仪(Bandelin Sonoplus UV 2200超声仪,装有BB5杯状管,在100%动力输出10秒脉冲)将基因组DNA片段机械剪切成大小范围150和300bp之间的片段或通过温和的DNase I处理(Novagen)剪切成大小50至70bp之间的片段。观察到当将DNA打碎成150-300bp大小范围的片段时,超声得到了紧凑很多的片段大小分布。然而,虽然DNA大量暴露于超声波诱导的流体力学剪切力,还是不能有效率并可重复的获得随后片段大小的降低。所以,使用Novagen的鸟枪(shotgun)剪切试剂盒通过温和的DNase I处理获得大小50至70bp的片段。制备试剂盒提供的1∶20稀释度的DNase I,在MnCl2存在下在60μl体积中在20℃进行消化5分钟以确保酶的双链剪切。用2μl 0.5M EDTA中止反应,在2%TAE-琼脂糖凝胶上评价片段化的效率。这个处理导致了基因组DNA片段化为近50-70bp片段。然后使用T4DNA聚合酶在100μM每个dNTP的存在下平端处理(blunt-ended)片段两次以确保末端的有效净化(flushing)。片段在连接反应中立即使用或为了随后的使用冷冻于-20℃。
载体的描述。在有与卡那霉素抗性基因交换的β-内酰胺酶(bla)基因的pASK-IBA骨架上{Skerra,A.,1994}构建载体pMAL4.31。另外,将bla基因克隆进多个克隆位点。编码成熟的β-内酰胺酶的序列先于ompA的先导肽序列以允许有效的跨细胞质膜的分泌。此外,编码成熟的β-内酰胺酶最初12个氨基酸(间隔子序列)的序列跟在ompA先导肽序列后面以避免紧接在先导肽酶切割位点之后的序列的融合,因为例如这个区域的正性电荷氨基酸群将降低或取消跨细胞质膜的转位{Kaiava,A.等人,2000}。SmaI限制性位点供给文库的插入。用于挑选片段的重新获得的上游FseI位点和下游NotI位点在SmaI位点的两侧。以bla基因转录在-1阅读框在NotI位点后得到一个终止密码子15bp的方式在编码12个氨基酸间隔子序列的序列之后插入三个限制性位点。+1bp插入重建了bla ORF以至于得到β-内酰胺酶伴随着氨苄青霉素抗性的获得。
通过将lamB基因克隆进pEH1的多个克隆位点构建载体pMAL9.1{Hashemzadeh-Bonehi,L.等人,1998}。随后,序列在氨基酸154后插入lamB,含有限制性位点FseI、SmaI和NotI。以挑选了框架的DNA片段的转移得到fhuA的连续阅读框和各自插入的这个方式构建这个插入的阅读框,所述DNA片段通过用来自质粒pMAL4.31的FseI和NotI的消化进行剪切。
通过将fhuA基因克隆进pEH1的多克隆位点构建了载体pHIE11。之后,序列在氨基酸405后插入fhuA,含有限制性位点FseI、XbaI和NotI。以挑选了框架的DNA片段的转移得到lamB的连续阅读框和各自插入的这个方式选择这个插入的阅读框,所述DNA片段通过用来自质粒pMAL4.31的FseI和NotI的消化进行剪切。
用于框架挑选的文库的克隆和评价。基因组粪肠球菌DNA片段连接到载体pMAL4.31的SmaI位点。将重组DNA电穿孔进DH10B电穿孔胜任的大肠杆菌细胞(GIBCO BRL),转化株接种于补充了卡那霉素(50μg/ml)和氨苄青霉素(50μg/ml)的LB琼脂平板上。平板在37℃孵育过夜,为了大规模的DNA提取收集克隆。储存和保留代表性的平板以收集克隆用于菌落的PCR分析和大规模的测序。简单的菌落PCR试验用于最初测定大致的片段大小分布以及插入效率。从测序的数据评价精确的片段大小、在插入位点的接合的完整性以及框架选择的精确性(3n+1规则)。
用于细菌表面展示的文库的克隆和评价。从pMAL4.31载体切割基因组DNA片段,含有有限制性酶FseI和NotI的粪肠球菌文库。然后完整的片段群转移到质粒pMAL9.1(LamB)或pHIE11(FhuA)中,所述质粒已经用FseI和NotI消化。使用识别8bp富含GC序列的这两个限制性酶,在每个平台载体中维持pMAL4.31载体中挑选的阅读框。然后通过电穿孔将质粒文库转化进大肠杆菌DH5α细胞。细胞接种于补充了50μg/ml卡那霉素的大的LB琼脂平板上,在可以得到清晰可见的单个菌落的密度在37℃生长过夜。然后从这些平板的表面刮下细胞,用新鲜LB培养基清洗,分装在-80℃储存用于文库筛选。
结果
用于框架挑选的文库。在pMAL4.31载体中产生分别有大约70和300bp大小的三个文库(LEF-70和LEF-300)。对于各个文库,大约1μg的pMAL4.31质粒DNA和50ng的片段化的基因组粪肠球菌DNA的连接和其后的转化在框架挑选后得到了1.5×105至1×106个克隆。为了评价文库的随机性,测序了大约576个LEF-70的随机选择的克隆。其中有483个被成功的修齐并用于随后的生物信息分析。生物信息分析显示这些克隆中只有很少的克隆多于一次存在。此外,显示大约73%的克隆的大小在范围25和100bp之间,有平均大小大约82bp(图2)。几乎所有序列遵循3n+1规则,显示了所有克隆恰当地选择框架。
细菌表面展示文库。大肠杆菌表面上肽的展示要求来自LSPy文库的插入体从框架挑选载体pMAL4.31转移到展示质粒pMAL9.1(LamB)或pHIE11(FhuA)。FseI和NotI限制性剪切基因组DNA片段,5ng插入体与0.1μg质粒DNA连接,其后转化进DH5α细胞得到了7.5×105-2×106个克隆。从LB平板刮下克隆并冷冻而没有进一步的扩增。所述细胞用做随后筛选步骤中的文库。
实施例3:使用细菌表面展示的基因组文库和人血清鉴别来自粪肠球菌的高度免疫原性肽序列
试验过程
MACS筛选。来自给定文库的大约2.5×108个细胞在37℃在5ml补充了50μg/ml卡那霉素的LB培养基中生长2小时。1mM IPTG加入30分钟诱导表达。用新鲜LB培养基清洗细胞两次,大约2×107个细胞重新悬浮在100μl LB培养基中并转移到Eppendorf管中。
20μg生物素化的从血清纯化的人IgG加入到细胞,悬浮液在4℃轻微震摇孵育过夜。加入900μl LB培养基,混合悬浮液,随后在6,000rpm4℃离心10分钟(对于IgA筛选,使用10μg纯化的IgA,这些用生物素化的抗人IgG二抗捕获)。用1ml LB清洗细胞一次,然后重新悬浮于100μl LB培养基。加入10μl偶联于链亲和素的MACS微珠(MiltenyiBiotech,德国),继续在4℃孵育20分钟。之后加入900μl LB培养基,将MACS微珠细胞悬浮液上样到已经平衡的固定于磁铁的MS柱(Miltenyi Biotech,德国)。(MS柱通过用1ml 70%EtOH清洗一次并用2ml LB培养基清洗两次平衡)。
然后用3ml LB培养基清洗柱三次。磁铁去除之后,通过用2ml LB培养基清洗洗脱细胞。用3ml LB培养基清洗柱后,2ml洗脱物第二次上样到相同的柱上,重复清洗和洗脱步骤。进行第三次上样、清洗和洗脱步骤,得到2ml的终洗脱物。
第二轮筛选进行如下。离心收集终洗脱物的细胞,重新悬浮在1ml补充了50μg/ml卡那霉素的LB培养基。培养物在37℃孵育90分钟,然后在1mM IPTG诱导30分钟。随后收集细胞,用1ml LB培养基清洗一次,悬浮于10μl LB培养基。对于第二轮筛选,加入相同量(20μg)的人生物素化的IgG,悬浮液在4℃轻微震摇孵育过夜。所有进一步的步骤与第一轮挑选完全一样,除了洗涤、上样和洗脱循环的数量依照每次筛选加以调整。两轮挑选之后挑选的细胞接种于补充了50μg/ml卡那霉素的LB琼脂平板上,37℃孵育过夜。
通过测序和Western斑点分析对挑选克隆的评价。挑选的克隆在补充了50μg/ml卡那霉素的3ml LB培养基中37℃生长过夜以使用标准程序制备质粒DNA。在MWG(德国)或与TIGR(美国)合作进行测序。
对于Western斑点分析,用10%SDS-PAGE分离大约10至20μg总细胞蛋白质,转印到HybondC膜(Amersham Pharmacia Biotech,英格兰)上。使用人血清作为第一抗体在大约1∶3,000至1∶5,000的稀释度以及偶联于HRP抗人IgG或IgA抗体作为第二抗体在1∶5,000的稀释度检测LamB或FhuA融合蛋白质。使用ECL检测试剂盒(AmershamPharmacia Biotech,英国)进行检测。或者,兔抗FhuA或兔抗LamB多克隆免疫血清用作第一抗体,联合各自的偶联于HRP的第二抗体以检测融合蛋白质。
结果
使用生物素化Ig通过磁铁激活的细胞分拣(MACS)对细菌表面展示文库的筛选。
来自健康个体(表1a)或从恢复期心内膜炎患者(表1c)的血清的生物素化的人IgG合并物筛选pMAL9.1中的LEF-70和pHIE11中的LEF-300文库(参见,实施例1:从人血清制备抗体)。如实验过程下描述地进行挑选过程。图3A显示了用LEF-70文库和IC10-IgG的筛选的代表性实例。如从菌落计数可以看到地,在从MACS筛选的第一挑选循环之后,最后重新获得的细胞总数戏剧性地从1×107个细胞降到大约5×104个细胞,但是没有抗体加入的挑选显示了相似的细胞数的降低(图3A)。对于第二轮,2×105个细胞上样于柱,在4个循环的上样、洗涤和洗脱后,几乎所有(1×105)的用IC10-IgG回收,而当不加入人血清的IgG时,仅有低于100个细胞被回收。结果清楚地显示了挑选依赖于粪肠球菌特异性抗体。通常,挑选导致约30%-70%克隆被用所加的血清IgG特异性的挑选。对于每次筛选,将400-1200个克隆进行DNA测序,所得序列与粪肠球菌V583的基因组相比对(blast)。生物信息分析允许基于通过加入人IgG所选的各个克隆的频率评价每次筛选。为证实筛选的良好实施,选择所有多于一次被挑选的克隆,用相同的合并的血清IgG进行Western印迹分析(图3B)。对更为频繁选择的克隆(>5X)的分析揭示了多于90%的所挑克隆显示了与相关血清中存在的抗体的反应性,而表达没有粪肠球菌特异性插入物的LamB的对照株与相同的血清IgG不反应。一般,以较低频率(<5x)挑选的克隆显示与所施加的血清IgG的低反应性百分比。菌落PCR分析显示了所有挑选并测试的克隆含有期待大小范围的插入物。
随后大量随机选择的克隆(每轮筛选400至1200个)的测序导致了基因和被用于筛选的人血清抗体特异性识别的相应的肽或蛋白质序列的鉴别。挑选特异性克隆的频率至少部分反映了在用于挑选的血清中并且识别这个克隆呈现的表位的特异性抗体的丰度和/或亲和力。在这点,很惊人地源自一些ORF的克隆(例如,EF3060,EFA0042)被选择了多次,且在多于一次的筛选中被选择,显示它们高度免疫原的特性。表1a总结了用来自健康成人的血清实施的四次筛选所获得的数据,表1c是用恢复期心内膜炎患者血清的四次筛选。后一表中仅含有相对于表1a所列出的而言为新的抗原。通过两类血清鉴别了26个抗原,分别从健康成人和患者的血清中挑选了71和42个独特型抗原ORF。
所有表1中显示的克隆已经通过Western印迹验证,方法是利用来自单一克隆的完整细胞提取物以显示与在各个筛选中所用人血清合并物的所示反应性。如表1所示,所鉴别的ORF的独特区被鉴别为免疫原性的,因为不同大小的蛋白质片段被在平台蛋白质的表面上展示。
更加值得注意多数细菌表面展示筛选鉴别的基因编码附着于粪肠球菌的表面的和/或是分泌的。这个与表面附着的或分泌的蛋白质在粪肠球菌毒力中期望的作用是一致的。
表1b显示通过与表1a中所列出的粪肠球菌抗原至少70%同一性的同源性检索鉴别的屎肠球菌蛋白质。用计算机程序TBLASTN鉴别粪肠球菌与屎肠球菌序列之间的同一性(http://www.hgsc.bcm.tmc.edu/microbial/microbialblast.cgi?organism=Efaecium)。基于相应的粪肠球菌抗原的完整ORF序列计算氨基酸序列的同一性。所得结果显示屎肠球菌含有与临床密切相关的粪肠球菌在结构上相关的蛋白质。由于粪肠球菌的同源性蛋白业已在本发明中提供为超免疫血清反应性抗原,表1b中给出的屎肠球菌蛋白质提供了相同的用途,特别是用作包含在药物组合物,例如针对肠球菌感染的疫苗,中的抗原。
实施例4:高度免疫原性肽序列与个体人血清反应性的评价
实验过程
肽合成
使用Syroll合成器(Multisyntech,Witten,德国)在Rink酰胺树脂上(PepChem,Tübingen,德国)使用标准F-moc化学小规模(4mg树脂;平行最多288个)合成肽。序列组合后,用Fmoc-ε-氨基己酸(作为连接剂)和生物素(Sigma,St.Louis,MO;象正常的氨基酸被激活的)延长肽。用93%TFA、5%三乙基硅烷和2%水1小时将肽从树脂上切下来。真空下干燥肽,从乙腈/水(1∶1)冷冻干燥3次。正确质量的存在通过质谱在Reflex
酶联免疫实验(ELISA)
根据生产厂商的说明书在链亲和素ELISA平板(EXICON)上在10μg/ml浓度包被生物素标记的肽(在N-末端)。根据生产厂商的推荐使用高度特异性辣根过氧化物酶(HRP)连接的抗人IgG二抗(Southern Biotech)(稀释度:1,000x)。在200X和1,000X两个血清稀释度测试血清。手工包被之后,通过有内置ELISA阅读仪(GENIOS,TECAN)的Gemini 160 ELISA机器人(TECAN)处理和分析肽平板。
结果
对挑选克隆的生物信息分析之后,设计并合成相应的肽。在有多于26个氨基酸残基的表位的情况下,制造重叠肽。所有的肽用N-末端生物素标记进行合成并用作链亲和素包被的ELISA平板上的包被试剂。
为了证实所选表位的免疫原性,通过利用个体人血清(其包括在用于细菌表面展示筛选的所述表位检定中的IC9-和IC10 IgG合并物中)的肽ELISA测量特异性抗体水平。显示了存在于61个粪肠球菌抗原中的104个肽与10个健康成人筛选血清(N1-N10)的血清反应性和与来自不同粪肠球菌感染的11个血清(P1-P11)的血清反应性的总结。通过基于阳性血清数和反应的程度计算的每个肽的分数对肽进行比较。肽在对弱阳性血清有很高的反应性到有广泛的反应性的范围内。在最高得分的肽表位(来自表面蛋白质)中,可见如LPXTG细胞壁蛋白质(EF0490.3和EFA0042.1-2),分泌的蛋白质(EF0360.1和EF0792.1)以及假设蛋白质(EF0428.1-2,EF3207.2和EFC0034.1-2)。有趣的是,两个主要表位属于替代阅读框ARF0679(44个氨基酸长)和ARF2052(54个氨基酸长),提示这些蛋白质确实存在,且在体内聚集和/或感染过程中表达。如相对于高滴度健康血清的患者血清之低总粪肠球菌抗体水平所示(图1C),与患者血清的肽反应性也较低。
表1A:细菌表面展示鉴别的免疫原性蛋白质。
粪肠球菌抗原蛋白质 | 推定的功能(通过同源性) | 推定的免疫原性氨基酸 | 每一ORF和筛选所选克隆号 | 鉴别的免疫原性区域的位置(aa) | Seq.ID(DNA,Prot.) |
EF0020 | PTS系统组分 | 4-10,14-21,30-36,59-68,77-82,87-93,96-105,112-121,125-133,135-141,150-162,164-183,192-203,207-213,215-226,228-234,241-247,250-285,302-308 | B:1,C:10,D:1 | 135-148 | 1,171 |
EF0032· | 膜蛋白,推定 | 15-57,60-73,77-101,108-134,136-177,185-201,203-217,226-240,244-254,272-277,283-288,292-343,354-370,380-398,406-437,439-453,473-490,532-538,584-590,595-601,606-612,664-677,679-704,715-724,731-753,759-772,786-794,814-862 | A:1,C:2 | 657-684 | 2,172 |
EF0062 | 2,3-环核苷酸2-磷酸二酯酶,推定 | 4-9,15-36,41-47,54-60,75-81,114-120,131-146,152-158,174-182,194-202,208-215,218-226,255-271,276-285,290-295,302-311,318-328,330-344,352-359,365-377,388-395,398-405,426-432,439-449,455-500,505-513,531-537,542-552,554-561,587-595,606-612,718-734,763-771,775-782,792-801,805-812,822-828,830-843,849-863,876-894,905-911,919-926,935-947,949-958,968-979,1009-1016,1029-1045,1047-1056,1076-1081,1092-1106,1123-1133,1179-1200,1202-1211,1215-1223,1287-1299,1301-1306 | A:3,B:1,C:16,D:1 | 398-431,1224-1237 | 3,173 |
EF0149 | 聚集物质ASP1 | 17-47,74-80,90-97,126-133,137-148,167-173,179-185,214-223,250-255,270-283,329-338,342-350,352-358,360-367,372-383,398-404,411-421,426-432,435-446,452-462,472-479,515-521,582-592,611-618,623-629,642-659,666-673,678-689,704-725,732-737,744-757,768-789,824-834,842-849,862-868,877-887,904-916,923-928,941-947,962-974,982-992,1019-1030,1032-1044,1046-1052,1065-1075,1077-1087,1108-1121,1124-1132,1137-1151,1170-1182,1190-1206,1208-1214,1227-1233,1242-1251,1254-1273,1282-1298 | A:5,C:2 | 792-825 | 4,174 |
EF0196 | 位点特异性重组酶家族蛋白质 | 19-31,39-67,82-91,104-110,113-128,149-155,161-181 | C:3 | 137-155 | 5,175 |
EF0253 | 醛脱氢酶 | 6-18,54-63,69-85,110-127,142-156,158-167,169-211,238-246,248-257,276-311,339-349,371-380,385-391,394-403,421-438,451-456,483-489 | C:2 | 449-468 | 6,176 |
EF0270 | PTS系统组分 | 5-15,24-34,50-56,61-83,98-121,123-136,149-162,166-194,202-215,221-227,229-332,337-360,367-402,404-415,427-433,444-462,471-478,487-498,511-518,521-544,550-563,568-574,580-587,597-607,610-616,624-629 | A:1,B:2C:2,D:2 | 468-498 | 7,177 |
EF0298 | 离子转运ATPase,E1-E2家族 | 11-19,32-49,57-63,65-71,80-89,91-133,166-181,183-191,201-230,234-257,264-291,297-303,305-314,316-335,337-354,359-366,368-374,383-388,394-405,408-442,446-470,483-490,499-505,513-538,544-555,557-563,568-590,598-608,617-623,627-636,641-647,667-685,687-693,710-723,733-739,742-754,769-815 | D:24 | 366-388 | 8,178 |
EF0355 | 内溶素 | 4-16,30-35,42-53,67-76,82-87,101-108,112-130,132-138,147-152,161-183,187-208,218-225,265-281,295-303,305-317,322-334,338-357,360-368,370-383,387-394,400-419,421-430 | A:1,B:3 | 255-336 | 9,179 |
EF0428 | 保守假设蛋白质 | 19-27,36-47,59-66,76-83,101-112,118-125,142-147,162-180,185-196,225-240,246-263,286-304,314-319,327-333,353-367 | A:1,C:23 | 194-214 | 10,180 |
EF0485(EF0005) | 聚集物质 | 14-43,70-76,83-89,111-117,122-128,136-145,163-170,175-182,210-219,246-251,266-279,325-331,338-346,348-354,356-363,368-379,422-428,431-441,450-456,466-473,509-515,532-542,549-556,576-586,605-612,617-623,636-653,660-667,674-686,698-719,726-731,738-745,762-783,818-828,836-843,856-862,871-881,903-910,917-922,935-941,956-968,976-986,1013-1024,1026-1038,1059-1069,1071-1081,1102-1115,1118-1126,1131-1145,1164-1176,1187-1200,1202-1208,1221-1227,1236-1245,1248-1267,1273-1292 | C:3,D:4 | 252-287,805-844 | 11,181 |
粪肠球菌抗原蛋白质 | 推定的功能(通过同源性) | 推定的免疫原性氨基酸 | 每一ORF和筛选所选克隆号 | 鉴别的免疫原性区域的位置(aa) | Seq.ID(DNA,Prot.) |
EF0490 | LPXTG-基序细胞壁锚结构域蛋白质 | 4-18,21-28,37-43,56-70,101-113,131-140,142-150,162-170,172-184,193-204,209-227,233-238,246-264 | A:2,C:10 | 93-168 | 12,182 |
EF0517 | 2-脱氢泛酸2-还原酶,推定 | 14-20,44-50,61-70,77-96,99-106,129-142,168-181,187-196,205-221,225-241,277-296 | C:4 | 257-281 | 13,183 |
EF0570 | 渗透压敏感性K+通道感受器组氨酸激酶, | 18-29,43-54,64-76,78-84,88-103,125-149,159-176,198-218,230-242,256-271,279-285,287-293,300-306,325-331,344-351,357-364,371-397,400-414,419-464,485-515,517-526,529-537,548-553,573-580,584-590,603-620,639-661,676-681,687-700,716-761,772-780,785-790,795-803,823-836,848-853 | A:2 | 106-134 | 14,184 |
EF0584 | ABC转运子,ATP-结合蛋白质 | 7-13,19-42,44-51,55-75,87-97,99-110,112-118,129-135,141-156,158-178,213-220,230-286,294-308,323-338,345-352,355-365,370-392,394-419,437-446,454-460,474-497,515-526,528-546,569-575 | C:3 | 128-141 | 15,185 |
EF0668 | UDP-N-酰基胞壁酰丙氨酰-D-谷氨酸-2,6-二氨基庚二酸连接酶 | 12-20,24-33,45-70,73-84,86-94,103-116,118-124,135-142,163-170,176-200,202-224,226-234,237-248,250-262,265-287,296-307,334-341,347-356,361-369,382-396,405-415,418-427,431-439,443-449,452-461,467-474 | D:2 | 113-146 | 16,186 |
EF0792 | 渗透酶,推定 | 13-38,44-50,52-59,66-72,83-94,103-110,116-124,131-137,158-180,199-204,218-233,241-264,269-317,326-342,350-356 | C:2 | 70-86 | 17,187 |
EF0795 | 保守假设蛋白质 | 29-35,49-59,63-84,86-97,103-111,113-126,130-144,150-158,174-198,221-231,250-264,266-273,291-298,310-318 | A:1,C:3 | 70-90 | 18,188 |
EF0799 | 自溶素 | 19-25,28-52,60-66,71-76,131-142,149-155,157-178,181-213,218-223,237-242,250-257,260-266,272-279,282-290,321-330,373-385,393-407,441-453,461-475,509-521,529-542,577-589,,597-610,643-655,663-677,703-718,729-734 | A:8,B:5 | 358-464,495-570,604-685 | 19,189 |
EF0851 | 假设蛋白质 | 4-29,51-76,116-136,158-173,179-193,207-215 | C:2 | 86-111 | 20,190 |
EF0861 | 酰基转移酶,GNAT家族 | 5-23,45-70,79-90,93-107,114-122,142-151 | C:3 | 18-36 | 21,191 |
EF0922 | 保守假设蛋白质 | 9-51,68-120,133-149,158-180,186-206,211-220,222-237,248-293,296-310,317-339 | C:2 | 248-260 | 22,192 |
EF0996 | 细胞分裂蛋白质FtsA | 14-24,44-63,69-98,108-119,123-136,155-161,164-176,180-193,203-208,215-223,239-247,274-281,283-289,296-304,306-313,315-327,331-341,343-353,357-386,392-405 | C:1,D:3 | 205-246 | 23,193 |
EF1012 | PTS系统组分 | 5-13,16-23,36-42,53-63,70-83,96-102 | C:2 | 14-34 | 24,194 |
EF1026 | 保守假设蛋白质 | 4-13,19-35,49-56,59-76,83-107,121-134,144-153,157-164,166-186,194-202,209-216,231-253,257-264 | C:21 | 98-134 | 25,195 |
EF1032 | 柔红霉素抗性蛋白质 | 16-32,38-47,58-68,78-89,98-114,117-123,132-141,146-156,164-170,179-188,196-212,219-230,232-237,244-263,265-274,278-293,297-303,306-326,339-349,352-359,362-367,373-379,384-394,396-406,423-443,451-461,465-484,490-497,504-511,523-533,537-547,550-556,558-566,573-579,586-593,598-609,615-642,647-665,671-686,693-713,723-728 | A:2 | 332-378 | 26,196 |
EF1060 | 肽ABC转运子,肽-结合蛋白质推定 | 6-21,34-44,58-64,66-74,79-87,114-127,129-143,154-162,174-189,205-214,241-262,266-273,278-297,319-324,328-338,342-351,390-398,409-415,422-435,458-464,471-477,481-486,506-531,534-540,542-550 | A:4 | 315-389 | 27,197 |
EF1093 | LPXTG-基序细胞壁锚结构域蛋白质 | 4-28,39-45,52-58,69-82,93-115,122-128,135-140,146-163,177-192,209-215,221-232,271-284,331-337,341-352,360-378,383-390,392-401,409-422,428-435,462-470,474-480,482-496,531-539,541-549,551-560,562-569,576-582,598-618 | C:17 | 98-127 | 28,198 |
EF1141 | MutT/nudix家族蛋白 | 14-27,33-47,61-79,94-104,119-133 | C:2 | 36-60 | 29,199 |
EF1182 | 自诱导-2 | 11-22,29-40,48-62,68-73,96-106,108-118,125-149 | C:3 | 102-126 | 30,200 |
粪肠球菌抗原蛋白质 | 推定的功能(通过同源性) | 推定的免疫原性氨基酸 | 每一ORF和筛选所选克隆号 | 鉴别的免疫原性区域的位置(aa) | Seq.ID(DNA,Prot.) |
产生蛋白LuxS | |||||
EF1277 | 转录调控子 | 4-11,45-55,76-83,86-102,105-112,138-144,147-153 | D:9 | 20-48 | 31,201 |
EF1289 | 保守结构域蛋白质 | 12-20,28-56,62-68,72-82,93-99,101-107,120-133,135-145,178-186,208-232,279-292 | C:2 | 36-64 | 32,202 |
EF1386 | 甲酸/亚硝酸转运子家族蛋白质 | 6-14,23-48,65-82,92-134,140-181,188-219,228-238,244-253,255-261 | C:6 | 124-145 | 33,203 |
EF1404 | MutS2家族蛋白质 | 11-25,31-38,53-59,62-71,89-99,125-133,151-157,182-190,195-203,208-215,219-229,249-262,267-275,287-295,298-316,318-325,328-334,344-353,357-363,371-377,385-391,396-415,425-436,438-457,471-485,538-552,554-561,606-625,630-636,646-653,669-679,695-704,706-715,722-747,763-773 | C:6 | 714-738 | 34,204 |
EF1561 | shikimate 5-脱氢酶 | 10-29,33-45,50-60,70-79,83-95,118-124,136-157,176-184,192-205,207-216,223-234,240-246,258-268,275-283 | C:1,D:29 | 37-56 | 35,205 |
EF1584 | 半胱氨酸合酶A | 4-24,27-38,46-54,66-72,81-97,112-119,128-137,152-157,173-179,185-214,219-225,227-248,262-284,286-295,301-307 | C:6 | 117-134 | 36,206 |
EF1597 | 催化酶 | 26-43,49-56,60-71,74-82,87-98,110-116,131-146,154-164,169-178,183-189,205-214,241-246,255-268,275-292,305-314,316-323,326-340,346-363,397-402,419-429,440-446,452-461,467-475 | C:3 | 29-66 | 37,207 |
EF1601 | PTS系统组分 | 7-16,21-39,48-58,61-78,82-89,109-136,138-150,152-176,182-247,255-261,267-332,336-345,347-358,362-368,371-392,394-404,407-472,490-498,505-513,527-544,554-582,603-611,614-620,632-638 | A:1,B:2,C:9 | 500-523 | 38,208 |
EF1624 | 醛脱氢酶,推定 | 24-46,77-83,90-97,99-118,123-166,168-177,204-212,229-239,248-262,273-282,287-293,300-319,321-337,340-352,357-366,391-402,411-428,442-450,464-471,479-489 | D:2 | 19-40 | 39,209 |
EF1646 | 热击蛋白Hs1VU,ATPase亚基Hs1U | 9-23,25-34,53-58,70-86,90-97,99-116,118-128,131-141,185-191,228-233,237-253,255-261,264-271,273-280,302-312,319-349,351-359,362-369,376-383,387-394,398-406,419-434 | C:3 | 20-31 | 40,210 |
EF1692 | 假设蛋白质 | 15-22,37-43,71-87,105-115,121-127,135-142,152-158 | D:4 | 32-52 | 41,211 |
EF1741 | 代谢产物调节蛋白 | 6-12,18-29,37-47,50-58,65-83,85-91,94-99,108-123,142-150,156-163,183-193,215-222,242-249,252-258;261-270,285-308,318-326 | A:4 | 1-95 | 42,212 |
EF1798 | 假设蛋白质 | 9-61,65-133,144-155,166-173,175-221,233-276,278-313,329-368 | C:2 | 210-233 | 43,213 |
EF1817 | 丝氨酸蛋白酶同源物 | 11-29,33-39,46-51,65-93,107-113,134-143,147-154,166-177,181-188,214-220,233-243,263-269 | A:2,B:1,D:3 | 112-128 | 44,214 |
EF1823 | 自溶素 | 8-46,110-134,155-167,174-183,188-201,210-230,253-258,267-282,289-299,312-319,322-327,330-337,365-381,389-402,405-411,419-425,439-447,465-472,489-512,525-532,540-554,577-589,591-599,605-614,616-624,633-649 | D:7 | 503-529 | 45,215 |
EF1978 | DNA-3-甲基腺嘌呤糖基化酶 | 34-49,64-70,90-118,124-131,141-152,159-165 | C:6 | 112-128 | 46,216 |
EF2005 | 保守假设蛋白质 | 5-15,26-45,55-72,80-85,93-100,121-133,142-148,154-167,198-205,209-215,241-254,260-265,271-279 | C:2 | 244-270 | 47,217 |
EF2052 | 细胞分裂蛋白FtsK | 4-36,38-54,67-83,122-153,159-178,205-212,232-242,244-253,259-268,281-288,298-309,324-331,334-370,372-381,389-401,403-429,441-450,456-462,465-471,473-479,483-504,508-518,537-543,553-565,578-584,592-609,619-625,658-667,669-679,712-719,722-729,737-744,746-752,758-765 | C:6 | 180-226 | 48,218 |
EF2074 | ABC转运子,ATP-结合蛋白质 | 6-17,23-32,49-56,61-67,76-83,85-103,105-111,120-132,145-171,175-185,191-225,231-246 | C:23 | 99-128 | 49,219 |
EF2266 | 2-脱氢-3-脱氧磷酸葡糖醛缩酶 | 4-24,28-48,52-58,64-79,87-100,104-120,136-152,159-166 | C:4 | 150-163 | 50,220 |
EF2305 | toprim结构域 | 15-27,65-71,77-99,104-121,128-151,183-216,223-229,234-255,277- | C:10 | 77-97 | 51,221 |
粪肠球菌抗原蛋白质 | 推定的功能(通过同源性) | 推定的免疫原性氨基酸 | 每一ORF和筛选所选克隆号 | 鉴别的免疫原性区域的位置(aa) | Seq.ID(DNA,Prot.) |
蛋白质 | 287,296-308 | ||||
EF2306 | 保守假设蛋白质 | 8-18,44-76,102-109 | C:2 | 49-57 | 52,222 |
EF2307 | 保守假设蛋白质 | 5-14,28-40,42-51,54-60,77-83,89-100,117-124,146-172,176-204,216-231,237-244,267-278,324-334,342-348,396-401,427-433,438-450,452-457,465-471,473-481,491-500,509-5515,523-544,550-556,558-569,589-595,606-618,625-632,640-649,665-671,678-688,691-698,717-723,728-734,781-789,800-805,812-821,833-868,873-879,889-905,929-939,988-998,1046-1061,1073-1079,1089-1096,1115-1124,1132-1140,1172-1196,1220-1226,1231-1249,1269-1277,1287-1301,1307-1330,1350-1361,1369-1378,1387-1412,1414-1420,1422-1439,1484-1491,1513-1529,1552-1561,1576-1583,1606-1613,1617-1640,1647-1654,1665-1679,1686-1698,1709-1727,1736-1743,1750-1757,1771-1790,1801-1807,1817-1823,1831-1842,1859-1868,1870-1882,1884-1891,1900-1906,1909-1914,1929-1935,1952-1960,1974-1988,2002-2011,2032-2063,2071-2081,2116-2124,2139-2147,2149-2159,2163-2190,2209-2215,2245-2253,2282-2287,2331-2342,2360-2370,2379-2393,2402-2408,2414-2421,2423-2430,2433-2439,2442-2450,2472-2478,2485-2493,2495-2503,2506-2512,2547-2554,2558-2564,2615-2625,2637-2652,2692-2698,2700-2706,2711-2723,2731-2740,2748-2753,2756-2762,2765-2772,2781-2798,2810-2824,2844-2852,2885-2899,2912-2922,2937-2944,2947-2970,2988-2998,3016-3025,3032-3037,3062-3071,3129-3148,3156-3161 | A:8,B:1,C:2, | 530-607 | 53,223 |
EF2326 | 反转录酶 | 31-36,57-62,79-85,90-96,99-112,120-146,162-185,193-203,208-217,219-226,239-253,283-290,298-304,306-321,340-349,351-361,365-372,386-395,407-438,473-486,537-551,558-568,576-594,598-604 | D:40 | 75-95 | 54,224 |
EF2378 | DNA聚合酶III,alpha亚基,革兰氏阳性 | 14-19,24-30,34-42,45-52,54-64,66-82,95-105,107-118,126-163,171-177,184-201,210-215,260-269,273-279,288-304,321-327,358-364,370-375,380-387,394-404,407-413,421-431,436-451,465-474,504-511,531-552,578-587,614-626,629-636,638-671,691-715,719-729,733-745,752-759,768-777,785-792,794-802,805-824,844-854,867-880,885-891,893-902,907-924,939-948,955-964,966-975,987-1000,1012-1017,1023-1028,1050-1071,1083-1098,1102-1115,1133-1146,1170-1183,1204-1211,1213-1223,1262-1311,1313-1319,1346-1355,1366-1371,1383-1405,1409-1414 | D:38 | 776-819 | 55,225 |
EF2476 | 青霉素-结合蛋白质2,推定 | 12-27,30-38,54-61,64-74,82-96,103-110,117-125,134-140,147-158,185-201,218-225,232-253,265-280,319-325,350-362,366-372,376-386,464-483,485-490,511-521,531-537,542-559,564-574,593-609,613-619,637-642,668-677 | A:3,C:8 | 195-214 | 56,226 |
EF2556 | 琥珀酸脱氢酶/富马酸还原酶,黄素蛋白质亚基 | 4-21,59-67,73-79,84-91,141-151,186-197,203-214,222-227,237-245,255-260,281-292,294-311,336-344,346-355,422-437,459-466,484-491 | A:1,C:3 | 77-109 | 57,227 |
EF2563 | 保守假设蛋白质 | 10-45,52-61,63-70,74-102,112-122,124-132,164-178,181-205,212-240,246-256 | C:2 | 226-247 | 58,228 |
EF2570 | 黄嘌呤脱氢酶 | 38-50,53-63,78-87,89-111,126-152,169-176,179-183,193-228,254-267,275-282,288-304,309-318,325-341,346-353,358-367,384-395,404-427,429-435,456-465,467-501,510-521,523-536,541-548,552-560,563-584,589-595,597-620,625-637,639-645,661-666,712-729,734-741,743-750,775-806,809-816,818-840,842-850 | C3 | 693-714 | 59,29 |
EF2581 | 氧还原酶吡啶核苷酸二硫键 | 5-17,30-37,52-75,77-86,88-107,112-135,151-160,178-222,226-246,263-270,279-294,306-314,327-342,345-352,374-381,389-416,422-429,435-449,453-467,473-500,512-522,524-531,542-549,552-560,565-571,575-586,594-600,613-619,625-633,635-641,647-653,667-674,680-699,711-729,735-741,764-775,781-786,792-798,805-813,817-825,833-842,850-855,860-866,869- | A:1,C:7 | 533-562 | 60,230 |
粪肠球菌抗原蛋白质 | 推定的功能(通过同源性) | 推定的免疫原性氨基酸 | 每一ORF和筛选所选克隆号 | 鉴别的免疫原性区域的位置(aa) | Seq.ID(DNA,Prot.) |
910,917-930,949-990 | |||||
EF2617 | 核酸酶R | 7-14,39-46,61-74,83-89,93-99,110-121,136-150,172-180,182-200,207-216,223-236,238-251,265-271,280-288,294-309,320-336,339-354,362-377,383-389,401-407,435-441,446-453,460-465,472-487,499-511,518-528,533-540,557-570,572-587,631-637,643-658,663-669,672-678,681-687,695-706,714-728 | C:2 | 118-139 | 61,231 |
EF2682 | 保守假设蛋白质 | 5-19,24-30,56-64,69-79,93-100,102-111,117-123,125-133,174-182,185-199,205-224,268-275,311-336 | D:20 | 102-125 | 62,232 |
EF2703 | 转录调节子 | 6-35,39-45,57-62,80-85,92-106,117-122,126-171,214-223,253-260,268-273,285-291,295-306,315-320,325-336,361-366 | A:1,B:2,D:12 | 172-202 | 63,233 |
EF2724 | 内切葡糖酶推定 | 4-13,24-37,45-51,58-66,84-92,112-121,132-141,151-171,175-195,204-212,222-240,262-268,276-295,305-336,338-348,354-362 | C:2 | 160-183 | 64,234 |
EF2782 | 半乳糖-1-磷酸脲基转移酶 | 10-16,24-35,41-73,78-104,111-121,124-139,141-148,150-164,196-215,224-241,249-282,299-307,315-357,368-378,393-401 | D:2 | 345-367 | 65,235 |
EF2787 | 氰酸酶家族蛋白质 | 4-32,48-53,61-67,84-104,112-118 | D:21 | 106-130 | 66,236 |
EF2812 | 假设蛋白质 | 21-28,31-36,65-81,98-105,115-121,123-131,136-142,155-161,177-190 | D:15 | 201-232 | 67,237 |
EF2858 | 苏氨酰-tRNA合成酶 | 4-15,21-27,33-39,42-56,58-64,68-82,84-90,92-98,113-122,146-162,168-175,177-189,191-203,249-268,279-285,287-304,328-342,349-358,371-378,387-393,404-413,419-425,467-479,487-498,513-524,528-539,541-565,572-579,595-606,626-635,637-642 | C:8 | 612-626 | 68,238 |
EF2893 | 假设蛋白质 | 7-13,52-70,76-82,97-106,110-117 | D:6 | 13-45 | 69,239 |
EF2927 | 水解酶,卤酸脱卤酶样家族 | 5-10,12-48,59-64,87-102,107-128,131-140,154-161,165-171,173-215 | C:2 | 54-74 | 70,240 |
EF2951 | 假设蛋白质 | 4-11,19-28,34-40,74-81,87-98,126-147,163-171,184-193,205-213 | B:1,C:2 | 49-124 | 71,241 |
EF2961 | 核糖激酶 | 7-14,23-29,35-40,61-67,99-106,111-122,124-133,135-161,187-206,216-229,236-245,262-268,271-280 | C:2 | 256-273 | 72,242 |
EF2986 | ABC转运子,ATP-结合蛋白质 | 4-13,17-37,47-54,85-99,105-113,120-132,147-166,180-186,192-199,204-216 | C:2 | 127-144 | 73,243 |
EF2987 | 保守假设蛋白质 | 14-27,29-37,52-62,68-76,89-96,117-123,125-131,137-145,166-195,205-212,214-222,228-235,258-264,271-281,288-296,308-324,332-339,335-361,365-371 | A:1,C:2 | 268-293 | 74,244 |
EF3023 | 多糖裂解酶,家族8 | 4-21,30-42,54-60,78-85,90-110,141-147,160-168,176-185,194-206,218-225,230-245,251-261,287-293,295-304,320-326,334-347,351-362,386-402,413-423,427-433,439-453,456-477,480-493,507-513,526-539,574-581,591-598,600-609,614-632,655-665,685-691,703-712,742-747,757-775,797-803,813-819,823-829,880-887,901-906,930-944,948-958,962-968,971-995,1002-1009,1017-1023,1036-1053,1069-1081,1107-1124,1129-1152,1178-1195,1211-1223,1249-1266,1271-1288,1334-1340,1346-1367 | A:9,D:6 | 1-63,171-189 | 75,245 |
EF3041 | 肽ABC转运子,肽-结合蛋白质 | 4-22,52-63,70-75,94-104,112-125,133-141,176-199,209-216,244-259,287-299,336-352,366-372,386-399,421-436,444-449,457-466,481-487,506-529,531-540 | A:2,B:1 | 295-378 | 76,246 |
EF3051 | 假设蛋白质 | 9-30,43-49,58-75,86-96,119-131,138-147,162-167,181-201,208-214 | A:4,C:3 | 16-121 | 77,247 |
EF3060 | 分泌的抗原SagA,推定 | 4-27,52-58,80-90,92-100,108-114,118-143,169-176,189-198,247-261,281-287,307-317,323-329,352-363,372-381,396-411,413-426,429-440,442-450,456-461,468-479 | A:14,B:6,C:1 | 1-73 | 78,248 |
EF3073 | 信号肽I | 4-32,47-52,57-63,71-78,92-104,126-142,153-175 | B:3,C:3 | 145-163 | 79,249 |
EF3086 | 保守假设蛋白质 | 17-23,35-41,51-70,73-86,104-125 | C:2 | 105-129 | 80,250 |
EF3096 | 染色体分离SMC蛋白质,推定 | 25-32,41-50,75-85,87-103,115-122,138-149,164-171,188-210,212-220,224-234,256-273,288-299,304-310,330-336,357-365,382-390,399-405,414-421,440-446,454-461,480-486,502-514,518-540,543-553,561-567,572-580,582-588,595-630,633-651,672- | C:14 | 909-936,1001-1031 | 81,251 |
粪肠球菌抗原蛋白质 | 推定的功能(通过同源性) | 推定的免疫原性氨基酸 | 每一ORF和筛选所选克隆号 | 鉴别的免疫原性区域的位置(aa) | Seq.ID(DNA,Prot.) |
681,691-709,760-767,813-832,841-848,852-866,873-893,919-925,927-933,940-955,957-978,984-997,1000-1010,1035-1040,1044-1051,1058-1064,1081-1091,1097-1124,1129-1138,1144-1150,1158-1165,1170-1180 | |||||
EF3125 | 引发体蛋白质N | 4-12,19-26,31-41,49-64,66-86,101-117,119-127,134-142,152-161,163-172,179-188,209-218,234-241,276-291,294-300,307-320,324-341,346-356,373-387,389-397,410-416,418-436,444-454,460-472,481-486,500-507,511-535,541-549,553-559,579-586,602-607,613-620,628-640,654-663,671-678,681-691,709-722,741-754,766-774,778-786,797-803 | C:4 | 212-226 | 82,252 |
EF3177 | 假设蛋白质 | 4-10,15-27,34-54,60-73,79-88,101-115,120-136,154-162,167-172,222-240 | A:3 | 126-195 | 83,253 |
EF3183 | LPXTG-基序细胞壁锚结构域蛋白质 | 5-16,18-25,29-35,57-63,86-91,107-121,123-131,170-179,185-199,204-226,250-255,262-274,291-296,325-347 | A:2,B:1 | 1-38 | 84,254 |
EF3207 | 保守假设蛋白质 | 7-19,22-34,36-42,48-54,60-66,71-76,104-110,118-135,135-145,158-164,167-174,182-193,196-204,217-229,251-290,293-299,309-315 | C:1,D:5 | 288-318 | 85,255 |
EF3276 | 保守假设蛋白质 | 43-51,55-61,66-73,80-90,103-127,133-142,174-180,185-196,203-210,229-235,239-251,258-266,272-278,289-314,316-326,340-346,355-361 | A:1 | 14-27 | 86,256 |
EF3290 | 感受器纽氨酸激酶 | 4-25,27-33,35-41,52-74,76-89,99-124,138-144,146-159,167-182,184-191,193-206,211-223,232-240,249-257,270-279,281-287,293-310,322-341,347-356 | C:14 | 292-322 | 87,257 |
EF3295 | 假设蛋白质 | 5-13,28-38,43-60,67-72,98-116,122-134,137-151,167-174,177-195,197-216 | B:1,C:4 | 99-195 | 88,258 |
EF3319 | 柠檬酸裂解酶,alpha亚基 | 15-33,35-42,48-57,62-68,73-91,107-119,121-153,173-194,205-210,223-228,234-241,243-259,275-298,308-315,327-340,342-370,376-391,398-404,410-419 | A:1,C:50 | 71-122 | 89,259 |
EFB0002 | 转座酶 | 12-39,43-64,87-95,99-105,114-126,128-136,139-147,212-225 | A:1,C:3 | 107-141 | 90,260 |
EFA0041 | 保守假设蛋白质 | 6-33,40-45,60-75,79-86,121-129,131-137,161-167,172-178,186-195,203-212,236-244,257-264,278-294,306-312,345-358,368-381,386-395,404-410,412-418 | A:4,B:5,D:3 | 198-270 | 91,261 |
EFA0042 | LPXTG-基序细胞壁锚结构域蛋白质 | 18-31,34-41,50-56,69-83,99-106,129-141,147-153,159-168,170-178,190-198,200-212,221-232,237-255,261-266,274-292 | A:3,B:2,C:26 | 118-216 | 92,262 |
EFA0047 | 聚集物质前体 | 17-47,61-67,87-93,115-121,126-132,140-148,167-173,179-186,214-223,250-255,264-272,282-294,306-318,338-353,358-377,385-401,414-420,433-441,451-457,470-480,505-511,544-550,571-581,600-607,612-618,631-648,655-662,669-681,693-714,721-726,733-740,757-778,813-823,831-838,851-857,866-876,893-905,912-917,930-936,951-963,971-981,1008-1019,1021-1033,1035-1041,1054-1064,1066-1076,1097-1110,1113-1121,1126-1140,1159-1171,1182-1195,1197-1203,1216-1222,1231-1240,1243-1262,1268-1287 | A:4,D:2 | 738-828 | 93,263 |
EFC0015 | 假设蛋白质 | 19-28,40-46,51-57,68-74,81-87,98-108,111-121 | D:3 | 20-36 | 94,264 |
EFC0025 | 假设蛋白质 | 4-17,19-44,60-69,80-86,110-116 | A:1,B:1,C:2 | 33-60 | 95,265 |
EFC0034 | 假设蛋白质 | 8-16,18-28,42-50,53-75,79-86,94-99,122-128,136-142,149-163,166-173,198-212,254-272,288-295,304-318,324-329,343-348,351-364,367-383,389-395,411-420,427-436 | C:10,D:150 | 11-56 | 96,266 |
ARFC0021.1 | 保守结构域蛋白质 | 19-25 | D:5 | 6-24 | 97,267 |
ARFC0021.2 | 保守结构域蛋白质 | 6-39,59-68 | D:3 | 44-63 | 98,268 |
ARF0031 | 假设蛋白质 | 5-14,21-28,38-53 | B:1,C:2 | 29-41 | 99,269 |
ARF0066 | 假设蛋白质 | 4-13,31-41,56-65 | C:14 | 32-56 | 100,270 |
粪肠球菌抗原蛋白质 | 推定的功能(通过同源性) | 推定的免疫原性氨基酸 | 每一ORF和筛选所选克隆号 | 鉴别的免疫原性区域的位置(aa) | Seq.ID(DNA,Prot.) |
ARF0076 | 假设蛋白质 | 5-12 | A:1,C:8 | 4-21 | 101,271 |
ARF0180 | 假设蛋白质 | 4-18 | A:1,C:15 | 17-32 | 102,272 |
ARF0275 | 假设蛋白质 | 4-10,23-33 | C:2 | 14-30 | 103,273 |
ARF0283 | 假设蛋白质 | 26-34,44-53 | D:2 | 35-52 | 104,274 |
ARF0375 | 假设蛋白质 | 无 | C:3 | 1-19 | 105,275 |
ARF0679 | 假设蛋白质 | 4-17,23-30,32-37 | D:17 | 6-23 | 106,276 |
ARF0721 | 假设蛋白质 | 5-33,40-58,61-66 | C:6 | 45-66 | 107,277 |
ARF1090 | 假设蛋白质 | 15-41,61-67 | C:5 | 41-65 | 108,278 |
ARF1583 | 假设蛋白质 | 4-12,16-23,26-37 | C:4 | 10-29 | 109,279 |
ARF2052 | 假设蛋白质 | 23-39 | C:37 | 37-55 | 110,280 |
ARF2125 | 假设蛋白质 | 12-20 | D:8 | 38-55 | 111,281 |
ARF2307.1 | 假设蛋白质 | 22-37 | C:4 | 7-22 | 112,282 |
ARF2307.2 | 假设蛋白质 | 无 | C:2 | 3-14 | 113,283 |
ARF2323 | 假设蛋白质 | 6-16,43-65,71-76 | C:14 | 17-31 | 114,284 |
ARF2505 | 假设蛋白质 | 4-13,27-39,42-69 | B:1,C:2 | 17-32 | 115,285 |
ARF2802 | 假设蛋白质 | 4-12,26-39 | A:1,B:1,C:3 | 10-25 | 116,286 |
ARF2902 | 假设蛋白质 | 无 | C:2 | 2-31 | 117,287 |
ARF3079 | 假设蛋白质 | 6-38,49-62 | D:4 | 39-55 | 118,288 |
ARF3157 | 假设蛋白质 | 4-10,24-30 | A:1,C:2 | 2-19 | 119,289 |
ARF3182 | 假设蛋白质 | 12-17,25-46 | A:2,C:2 | 15-30 | 120,290 |
ARF3314 | 假设蛋白质 | 4-13 | A:2,B:1,C:3,D:1 | 2-28 | 121,291 |
ARFA0022 | 假设蛋白质 | 30-38 | C:2 | 17-45 | 122,292 |
CRF0022 | 假设蛋白质 | 24-33,55-61 | C:6 | 31-61 | 123,293 |
CRF0073 | 假设蛋白质 | 4-26,34-48 | D:2 | 15-33 | 124,294 |
CRF0096 | 假设蛋白质 | 9-15 | D:3 | 1-22 | 125,295 |
CRF0115 | 假设蛋白质 | 4-31 | C:2 | 14-33 | 126,296 |
CRF0202 | 假设蛋白质 | 5-34,49-55,64-82 | C:3 | 69-83 | 127,297 |
CRF0249 | 假设蛋白质 | 33-45 | D:2 | 21-39 | 128,298 |
CRF0258 | 假设蛋白质 | 7-14,24-32,42-65,79-86 | C:3 | 50-64 | 129,299 |
CRF0264 | 假设蛋白质 | 13-27,33-43,45-62 | C:3 | 12-37 | 130,300 |
CRR0339 | 假设蛋白质 | 4-15,17-32 | C:3 | 10-26 | 131,301 |
CRF0399 | 假设蛋白质 | 4-9,11-43,45-75 | C:4 | 47-69 | 132,302 |
CRF0682 | 假设蛋白质 | 4-18,22-37 | C:12 | 17-34 | 133,303 |
CRF0783 | 假设蛋白质 | 4-14 | D:6 | 5-24 | 134,304 |
CRF0801 | 假设蛋白质 | 7-33,35-46 | C:31 | 1-19 | 135,305 |
CRF0892 | 假设蛋白质 | 13-37,69-75 | C:9 | 51-69 | 136,306 |
CRF1041 | 假设蛋白质 | 14-24,26-34,37-49,66-78 | C:5 | 2-25 | 137,307 |
CRF1049 | 假设蛋白质 | 17-46,52-57,59-64 | C:3 | 54-68 | 138,308 |
CRF1327 | 假设蛋白质 | 4-22 | C:2 | 13-25 | 139,309 |
CRF1593 | 假设蛋白质 | 8-40,53-63 | D:3 | 29-50 | 140,310 |
CRF1610 | 假设蛋白质 | 16-28 | C:2 | 32-40 | 141,311 |
CRF1732 | 假设蛋白质 | 14-20,22-28,39-45 | C:5,D:9 | 2-22 | 142,312 |
CRF1830 | 假设蛋白质 | 4-13 | C:2 | 12-31 | 143,313 |
CRF1992 | 假设蛋白质 | 15-21 | D:11 | 2-17 | 144,314 |
CRF2074 | 假设蛋白质 | 4-17 | D:2 | 20-36 | 145,315 |
CRF2099 | 假设蛋白质 | 4-19 | C:2 | 9-18 | 146,316 |
CRF2298 | 假设蛋白质 | 4-14 | D:2 | 3-19 | 147,317 |
CRF2318 | 假设蛋白质 | 4-21,32-40 | C:2 | 21-39 | 148,318 |
CRF2568 | 假设蛋白质 | 4-13 | C:2 | 10-27 | 149,319 |
CRF2573 | 假设蛋白质 | 18-31,39-47,75-87,89-98 | C:3 | 79-99 | 150,320 |
粪肠球菌抗原蛋白质 | 推定的功能(通过同源性) | 推定的免疫原性氨基酸 | 每一ORF和筛选所选克隆号 | 鉴别的免疫原性区域的位置(aa) | Seq.ID(DNA,Prot.) |
CRF2581 | 假设蛋白质 | 15-21 | C:12D:29 | 9-4 | 151,321 |
CRF2647 | 假设蛋白质 | 4-14,18-27,30-53,55-64,68-74,81-98 | C:7 | 22-40 | 152,322 |
CRF2706 | 假设蛋白质 | 7-24,44-51 | C:2 | 35-60 | 153,323 |
CRF2751 | 假设蛋白质 | 10-47 | C:3 | 23-37 | 154,324 |
CRF2768 | 假设蛋白质 | 4-10,12-46 | C:3 | 7-22 | 155,325 |
CRF2778 | 假设蛋白质 | 20-27 | C:3 | 1-13 | 156,326 |
CRF2790 | 假设蛋白质 | 6-19,41-51 | C:12 | 9-37 | 157,327 |
CRF2899 | 假设蛋白质 | 4-9,11-17 | C:2 | 9-23 | 158,328 |
CRF2935 | 假设蛋白质 | 4-17,23-38,46-66,68-85 | D:26 | 34-46 | 159,329 |
CRF2966 | 假设蛋白质 | 4-18,34-59,75-81 | D:31 | 61-84 | 160,330 |
CRF3074 | 假设蛋白质 | 6-17 | C:2 | 7-28 | 161,331 |
CRF3084 | 假设蛋白质 | 4-32,56-61 | D:6 | 35-52 | 162,332 |
CRF3120 | 假设蛋白质 | 4-14,27-71,74-88,93-110,115-120,124-130,139-154,161-172 | C:4 | 146-171 | 163,333 |
CRF3276 | 假设蛋白质 | 4-21 | C:39 | 3-15 | 164,334 |
CRF3277 | 假设蛋白质 | 12-17 | C:11 | 9-26 | 165,335 |
CRF3281 | 假设蛋白质 | 10-21,45-58 | C:3 | 51-67 | 166,336 |
CRF3285 | 假设蛋白质 | 59-66,68-84 | D:2 | 13-42 | 167,337 |
CRFC0021 | 假设蛋白质 | 11-16 | C:3 | 1-16 | 168,338 |
CRFC0046 | 假设蛋白质 | 4-19,23-37 | C:4 | 10-30 | 169,339 |
NRF0001 | 假设蛋白质 | 19-27,35-46,48-66,82-88,99-105,113-119 | C:1 | 42-59 | 170,340 |
表1B:通过与鉴别的粪肠球菌抗原的同源性检索鉴别的屎肠球菌抗原
原肠球菌 | 推定的功能(与粪肠球菌的同源性) | 粪肠球菌抗原蛋白质 | Seq.ID(DNA,Prot.) | 与粪肠球菌的同一性(%) | Seq.ID(DNA,Prot.) |
EFN0001 | PTS系统组分 | EF0020 | 1,171 | 78 | 341,357 |
EFN0002 | 保守的假设蛋白质 | EF0428 | 10,180 | 80 | 342,358 |
EFN0003 | 2-脱氢泛酸2-还原酶,推定 | EF0517 | 13,183 | 77 | 343,359 |
EFN0004 | 保守的假设蛋白质 | EF0795 | 18,188 | 80 | 344,360 |
EFN0005 | 细胞分裂蛋白质FtsA | EF0996 | 23,193 | 78 | 345,361 |
EFN0006 | PTS系统组分 | EF1012 | 24,194 | 87 | 346,362 |
EFN0007 | LPXTG-基序细胞壁锚结构域蛋白质 | EF1093 | 28,198 | 74 | 347,363 |
EFN0008 | MutS2家族蛋白质 | EF1404 | 34,204 | 77 | 348,364 |
EFN0009 | 分解代谢物调节蛋白质 | EF1741 | 42,212 | 80 | 349,365 |
EFN0010 | 2-脱氢-3-脱氧磷酸葡糖酸醛缩酶 | EF2266 | 50,220 | 76 | 350,366 |
EFN0011 | DNA聚合酶III,alpha亚基,革兰氏阳性型 | EF2378 | 55,225 | 82 | 351,367 |
EFN0012 | 硫氰酸生成酶家族蛋白质 | EF2787 | 66,236 | 70 | 352,368 |
EFN0013 | 苏氨酰-tRNA合成酶 | EF2858 | 68,238 | 89 | 353,369 |
EFN0014 | 保守的假设蛋白质 | EF3207 | 85,255 | 85 | 354,370 |
EFN0015 | 感受器组氨酸激酶 | EF3290 | 87,257 | 73 | 355,371 |
EFN0016 | 转座酶 | EFB0002 | 90,260 | 83 | 356,372 |
表1c通过细菌表面展示用心内膜炎患者血清鉴别的免疫原性蛋白质
粪肠球菌抗原蛋白质 | 推定的功能(通过同源性) | 推定的免疫原性氨基酸· | 每一ORF和筛选的所选克隆号 | 鉴别的免疫原性区域的位置(aa) | Seq.ID(DNA,Prot.) |
EF0008 | 单链结合蛋白质 | 4-11,16-34,48-55,67-77,87-106 | E:8 | 153-183 | 373,425 |
EF0028 | IPTS系统组分 | 22-40,49-65,70-91,95-109,111-125,146-207,209-216,219-225,229-244,251-270,274-286,292-309,316-329,335-355,358-370,376-388,392-419,425-430,435-441,448-455,464-478,486-515 | F:1 | 437-465 | 374,426 |
EF0146 | 表面排出蛋白质Seal推定 | 5-19,25-31,43-48,60-79,88-100,105-129,148-171,187-193,243-263,316-322,334-340,345-351,369-378,381-391,399-404,474-483,502-517,525-530,558-568,579-596,622-627,631-638,644-651,653-660,674-680,687-693,721-728,743-753,760-775,788-795,806-813,821-828,835-842,847-859,868-887 | F:13,H:3 | 300-347 | 375,427 |
EF0153 | ILPXTG-基序细胞壁锚结构域蛋白质 | 5-26,37-44,89-97,112-118,121-128,138-154,157-165,176-181,188-198,205-218,223-243,247-253,260-279 | H:1 | 76-155 | 376,428 |
EF0394 | 与分泌的抗原SagA粪肠球菌44%同源性 | 4-29,41-46,49-68,82-88,121-147,158-164,187-193,195-208,229-236,244-249,251-263,269-275,307-313,337-343,348-381,392-398,402-408,432-438 | E:96,G:2 | 85-117,194-239 | 377,429 |
EF0443 | 与LysM结构域蛋白质同源性 | 5-12,14-22,28-34,40-46,70-79,84-129,152-165,174-182 | G:2 | 37-109 | 378,430 |
EF0568 | 钾转运ATPase亚基B | 5-16,18-52,54-72,81-86,118-126,136-145,151-157,168-180,209-233,244-270,295-302,315-326,329-337,345-352,364-373,397-402,408-418,424-431,435-443,472-480,483-489,504-510,519-527,549-564,576-599,605-637,641-673 | E:2 | 91-98 | 379,431 |
EF0591 | 脂蛋白推定 | 23-36,42-52,133-140,151-157,242-247,267-277,295-301,320-328,333-339,345-352,365-371,397-403,415-428,456-465,481-487,489-495,508-516,518-527,585-592,606-614,631-637,643-658,665-670,723-728,737-744,752-759,787-793,835-841,873-885,918-928,938-945,951-966,978-988,1015-1020,1030-1036,1044-1052,1058-1069,1071-1079,1081-1088,1113-1119,1125-1138,1141-1147,1164-1170,1172-1177,1190-1200,1214-1220,1230-1236,1239-1245,1262-1268,1270-1275,1288-1298,1312-1318,1328-1334,1337-1343,1360-1366,1368-1373,1386-1396,1410-1416,1426-1432,1435-1441,1458-1464,1466-1471,1484-1494,1508-1514,1524-1530,1533-1539,1556-1562 | F:3 | 307-340 | 380,432 |
EF0592 | LPXTG-基序细胞壁锚结构域蛋白质(重复结构域) | 19-25,35-41,44-50,66-72,74-79,92-102,116-122,132-138,141-147,164-170,172-177,190-200,214-220,230-236,239-245,262-268,270-275,288-298,312-318,328-334,337-343,360-366,368-373,386-396,410-416,426-432,435-441,458-464,466-478,504-524 | H:1 | 79-148,177-246,275-344,373-442 | 381,433 |
EF0658 | 假设蛋白质 | 7-14,16-23,33-39,46-53,72-79,92-115,123-130,156-175,179-187,214-220,239-246,266-274,302-325,338-354,360-370,375-390,392-401,421-428,430-463 | F:2 | 29-58 | 382,434 |
EF0727 | 保守的假设蛋白质 | 4-9,22-39,58-65,72-82,87-92,99-104,107-119,143-166,171-177,194-202,205-213,220-228,231-240,247-263,309-315,317-323,336-343 | E:1 | 294-320 | 383,435 |
EF0775 | 聚集物质-嵌合 | 4-10,12-18,24-29,34-43,50-65,70-76,111-117,129-138,152-159,166-171,184-195,200-210,224-236,241-251,274-283,285-296,313-319,332-341,348-355,378-386,410-416,433-445,475-482,523-529,531-540,584-596,626-633,674-680,682-688,738-750,780-787,828-834,836-842,853-862,882-887,893-912 | H:2 | 504-676 | 384,436 |
EF0779 | 保守的结构域蛋白质 | 15-38,49-57,60-99,103-119,124-194,200-206,215-249,251-291,307-313,315-347,369-378,383-390,393-400,405-411,423-435,440-447,454-460,470-486,490-503,532-539,542- | H:2 | 509-583 | 385,437 |
粪肠球菌抗原蛋白质 | 推定的功能(通过同源性) | 推定的免疫原性氨基酸· | 每一ORF和筛选的所选克隆号 | 鉴别的免疫原性区域的位置(aa) | Seq.ID(DNA,Prot.) |
549,551-567,579-592 | |||||
EF1091 | 假设蛋白质 | 38-44,47-88,95-103,157-172,235-240,250-260,263-276,294-300,312-317,331-337,369-391,412-419,442-448,453-463,490-529,537-555,571-580,600-617,619-627,642-648,682-687,693-700,716-722,738-748,756-763,779-789,796-802,820-828,833-840,846-853,862-872,880-887,894-899,924-937,957-963,1006-1012,1043-1049,1063-1069,1076-1097 | F:4 | 124-147 | 386,438 |
EF1323 | 保守的假设蛋白质真实点突变 | 4-28,31-49,60-71,75-102,104-114,134-144,160-184,250-257,277-285,287-294,330-338,345-351,367-374,381-388,393-399,402-407,420-426,443-448,458-464 | F:8 | 411-436,454-488 | 387,439 |
EF1355 | 丙酮酸脱氢酶复合物E2 | 20-27,45-55,57-64,66-77,98-106,130-137,155-165,167-174,176-187,194-203,208-223,227-238,245-251,257-270,273-278,287-299,330-345,352-358,363-385,392-399,410-417,437-443,467-484,486-492,495-500,504-516,526-536 | E:2 | 219-270 | 388,440 |
EF1699 | 转录调控子MerR家族 | 11-22,24-31,46-63,65-71,73-88,95-109,174-181,183-201,204-212,216-222,228-233,241-247 | H:2 | 142-221 | 389,441 |
EF1744 | 一般应激蛋白质 | 8-28,51-59,67-84,93-98,140-152,154-162,183-188 | F:3 | 91-125 | 390,442 |
EF1752 | 保守假设蛋白质 | 10-22,27-61 | F:6 | 69-100 | 391,443 |
EF1753 | 保守假设蛋白质 | 7-15,18-26,94-100,126-131,152-165,219-228,254-263,274-292,297-308,333-340,342-352,354-371,373-379,403-410,420-438,450-456,463-470,489-495,503-512 | G:2 | 97-173 | 392,444 |
EF1791 | 肽ABC转运子肽结合蛋白质 | 4-21,37-43,49-65,67-74,76-90,113-119,131-141,155-173,175-189,192-199,207-221,247-254,266-276,317-322,337-343,387-393,408-428,439-447,451-460,469-479,482-487,493-501,517-523,533-542 | F:1 | 480-503 | 393,445 |
EF1800 | 保守假设蛋白质 | 11-26,40-46,78-86,93-103,121-126,132-138,166-177,183-196,203-212,214-221,228-263,304-311,323-338,345-351,357-363,379-393,420-434,442-448,518-527,547-553,581-591,602-609,637-645,665-674,687-692,701-708,730-739,796-802,844-857,882-888,903-914,944-950,976-983,1027-1033,1049-1057,1066-1072,1085-1092,1120-1127,1137-1144,1153-1158,1165-1176,1181-1187,1221-1230,1238-1244,1269-1274 | E:1 | 605-632 | 394,446 |
EF1818 | 明胶酶 | 6-47,57-65,83-95,109-121,138-147,154-164,167-177,194-200,202-212,227-234,240-253,260-267,283-291,320-329,340-347,356-364,412-422,430-436,441-459,465-475,478-486,498-507 | F:14 | 59-84 | 395,447 |
EF1850 | 保守假设蛋白质 | 10-21,58-83,88-97,120-126 | F:1 | 21-51 | 396,448 |
EF1877 | 保守假设蛋白质 | 5-39,56-62,76-88,90-114,138-162,170-195,202-221,228-250,264-270,304-355,374-387,391-416,462-471,526-546554-561,674-579,639-645,651-660,674-682,689-694 | E:10 | 666-697 | 397,449 |
EF2174 | 假设蛋白质 | 6-30,36-42,143-157,176-197,202-209,216-233,241-246,275-287,292-299,315-325,343-350,375-380,397-403,411-420,422-434,441-448,467-474,477-499,555-568,591-597,601-609,623-644,667-688,692-698,703-718,736-747,757-766,782-791,795-801,832-840,859-865 | F:119 | 226-269 | 398,450 |
EF2224 | LPXTG-基序细胞壁锚结构域蛋白质 | 6-23,43-51,61-67,73-82,91-97,123-130,149-158,164-175,228-234,240-246,248-255,262-272,326-332,340-347,365-371,377-388,409-419,425-431,438-445,449-457,464-470,496-507,559-568,575-581,603-608,617-623,633-639,648-654,659-670,695-701,734-752,806-814,816-829,861-868,891-899,904-909,937-945,947-960,978-983,992-999,1022-1031,1068-1076,1078-1091,1109-1114,1123-1130,1153-1162,1199-1207,1209-1222,1254-1261,1284-1293,1330-1338,1340-1353,1371-1376,1385-1392,1415-1421,1433- | F:2 | 1422-1458 | 399,451 |
粪肠球菌抗原蛋白质 | 推定的功能(通过同源性) | 推定的免疫原性氨基酸· | 每一ORF和筛选的所选克隆号 | 鉴别的免疫原性区域的位置(aa) | Seq.ID(DNA,Prot.) |
1438,1460-1465,1470-1492 | |||||
EF2318 | 肽酶M23/M37家族V | 82-94,111-118,125-131,206-212,261-266,310-320,328-338345-351,353-360,414-420,424-434,440-447,451-500,506-516,584-561,566-572,584-591,601-622,630-636,650-659,661-667,674-699,703-711,717-729,736-744,752-759,765-771,813-822,826-842,852-868,870-877,881-895,897-906913-922 | H:5 | 602-671 | 400,452 |
EF2704 | A/G-特异性腺嘌呤糖基化酶 | 12-18,20-25,43-54,56-65,73-79,82-88,99-111,136-142,153-169,171-183,195-223,229-248,255-260,272-277,281-292,298-319,322-329,332-351,363-379,381-389 | F:5 | 275-304 | 401,453 |
EF2713 | LPXTG-基序细胞壁锚 结构域 蛋白质 | 4-9,34-48,65-77,101-106,111-131,138-153,186-191,230-250 | H:1 | 148-219 | 402,454 |
EF2802 | 内溶素 | 4-23,30-35,42-53,67-76,82-87,101-108,112-130,132-138,147-152,161-183,187-208,218-225,265-283,295-303,306-317,322-334,338-357,360-368,370-383,387-398,400-419421-430 | H:2 | 104-182,240-304 | 403,455 |
EF2813 | 保守假设蛋白质 | 4-12,63-69,94-102,146-164,166-173,175-181,193-207,263-281,286-295,301-306,330-343,369-378,382-388,414-420,422-430,438-454,456-462,472-531,543-560,581-591,596-605,614-623,626-635,656-662,669-676,683-690,693-698,705-711,728-736,752-764 | E:4 | 69-102 | 404,456 |
EF2820 | 假设蛋白质 | 6-12,43-53,141-147,164-179,185-195,197-206,227-235,237-271,288-305,308-317,335-341,351-357,365-376,386-395,397-416,422-447 | E:1 | 11-35 | 405,457 |
EF3082 | 铁化合物ABC转座子底物结合蛋白质 | 16-24,50-65,73-84,88-99,114-124,130-146,181-187,193-203,214-220,236-247,250-258,287-297 | F:92 | 50-113 | 406,458 |
EF3256 | 脂蛋白推定 | 4-25,50-55,76-82,117-123,131-137,139-148,158-166,239-245,253-258,266-275,277-292,300-306 | F:5,G:3 | 51-83,93-161 | 407,459 |
EFA0021 | 假设蛋白质 | 6-22,34-43,51-86,93-100,110-116,150-161,164-171,180-187,197-218 | H:2 | 168-237 | 408,460 |
EFA0044 | 假设蛋白质 | 4-27,55-60,74-82 | E:6 | 10-46 | 409,461 |
EFA0052 | 表面排出蛋白质Sea1 | 6-19,25-31,43-49,60-79,88-100,105-129,148-161,164-171,187-193,243-263,316-322,334-340,369-378,381-391,398-404,460-466,474-483,502-509,511-517,525-530,558-567,579-596,622-627,631-638,641-651,653-659,674-680,687-693,710-716,720-727,743-753,760-775,788-795,806-813,821-828,836-842,847-860,865-880 | H:10 | 258-377 | 410,462 |
EFC0004 | TraC蛋白质 | 4-11,25-64,71-79,88-94,107-120,123-132,167-188,231-237,240-246,261-267,306-311,330-342,351-358,389-395,406-418,429-434,439-448,483-501,511-520 | G:1 | 71-143 | 411,463 |
EFC0012 | LPXTG-基序细胞壁锚结构域蛋白质 | 4-18,22-27,53-64,94-100,121-127,133-139,155-164,177-182,187-196,206-218,224-242,248-253,258-277 | H:1 | 184-253 | 412,464 |
EFC0021 | 保守结构域蛋白质 | 10-17,56-67,72-82,94-99,106-113,166-173,229-235,243-283,295-301,313-321,326-331,342-348,396-414,423-435,446-452,454-462,496-502,511-534,543-556,563-570,586-593,616-626,638-645,653-662,679-696,731-737,766-774,776-782,790-796,810-817,825-835,837-846 | H:5 | 540-615 | 413,465 |
EFC0053 | 转座酶突变子家族 | 13-24,30-36,73-81,89-95,109-115,117-143,161-173,179-189,226-244,251-261,275-281,298-305,307-315,323-328,364-374 | G:6,H:12 | 69-186,264-354 | 414,466 |
ARFC0021.1 | 假设蛋白质 | 19-25 | D:5 | 6-22 | 415,467 |
ARFC0021.2 | 假设蛋白质 | 6-39,59-68 | D:3 | 43-62 | 416,468 |
ARF0324 | 假设蛋白质 | 6-14,22-32 | F:2 | 1-27 | 417,469 |
ARF1627 | 假设蛋白质 | 4-41 | E:127 | 28-40 | 418,470 |
ARF1650 | 假设蛋白质 | 8-14 | F:5 | 4-19 | 419,471 |
CRE0097 | 假设蛋白质 | 4-10,12-22,30-35 | E:78 | 6-33 | 420,472 |
粪肠球菌抗原蛋白质 | 推定的功能(通过同源性) | 推定的免疫原性氨基酸· | 每一ORF和筛选的所选克隆-号 | 鉴别的免疫原性区域的位置(aa) | Seq.ID(DNA,Prot.) |
CRF0257 | 假设蛋白质 | 4-16,24-33 | F:21 | 37-54 | 421,473 |
CRF0635 | 假设蛋白质 | 无 | E:7 | 2-23 | 422,474 |
CRF1152 | 假设蛋白质 | 4-21,27-33,36-41 | E:16 | 14-34 | 423,475 |
CRF1720 | 假设蛋白质 | 4-14,24-30,37-42,57-78,83-89,94-103,113-131 | E:5 | 100-122 | 424,476 |
表2:人血清的表位血清学
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序列表
Seq ID 1
atggtaggaa ttatcctagc aagtcacggt gaatttgctg aagggatctt gcaatctggt 60
gcaatgatct ttggcgaaca agaaaatgta aaagcagtta cgttaatgcc tagcgaaggt 120
cccgatgatg taaaagcaaa aatgcaagaa gccattgcat cgtttgacaa tcaagacgaa 180
gtactatttt tagttgacct atggggagga acaccattta accaagcgaa ctcattgtta 240
gaagatcata aagacaaatg ggcaatcgtt gcaggtatga acttaccaat ggttattgaa 300
gcatatgctt ctcgtttctc tatggaatca gcacaagaaa ttgcgacaca cattcttgaa 360
acagcaaaag atggtgtcaa agttaaacta gaagagttac aaccagcaga agcaccaaaa 420
gctgctgcga cagaagatgc acagccaaaa ggttcgttac caccaggtac agttgttggc 480
gatggcaaaa ttaaatttgt gctagcacgt attgattcac gtttattaca tggtcaagta 540
gcaacagctt ggacaaaagc aacacaacca aacagaatta ttgttgtttc tgatgccgtt 600
gcaaaagatg acttacgtaa gaaattaatc gaacaagcag ctcctccagg agtaaaagcc 660
aatgttattc caatcagtga aatgattgaa gtagcgaaag atccacggtt tggcaataca 720
aaagcattat tattatttga aaatcctgaa gacgtcttaa aagtggttga aggcggcgta 780
gaaatcccag aagtaaacgt tggttcaatg gctcactctg ttggaaaagt cgttgtaagc 840
aaagtattat caatgggtca agaagacgtt gatacgtttg atgaattaaa agccaaaggc 900
atcaaatttg atgtgcgtaa agtgccaaat gattcaaaag cgaatatgga cgaaattttg 960
aaaaaagcaa aaaatgagtt agcaaacgcg 990
Seq ID 2
atgaaaaata aaagtaggca gtttttaaaa aacaactggc cttatatgtt agccagtttc 60
ttcattccat ttttaattat ggcaattatc tatttgagta ttgggattta tccaggaagt 120
agtcgcagtg ttttagccag cgatgccttt tctcaatttt caaattttca cgcaagcttt 180
aacaatgttc ttcatggaaa acaaagtctt ttctatacgt ggaatgcttc tttgggacta 240
aactacttgt ccttaatttc ctattatctt ggtggcttat ttacgccgct cgtctttttc 300
tttaacaatc aaaacatgcc tgatgcgctc tatttcctaa cgttgttgaa aattgggagt 360
gctggcttga gtttttggtt cttggcaaaa caaaccttta agattcctaa atggtcccat 420
gtgactttaa gtgtgtctta tgcgctaatg tccttcattg tggcccattc agaattaatt 480
atgtggctgg atgcctttat ttatctgcca ctcgtaattt taggaattca tcgtttaatg 540
gaccaacgaa aaccgacatt attgtttgtc agttatttct tgcttttcat tacaaattat 600
tattttggct ttatgattgg gttgttttcc ttcctgtact attttgcacg aacgtttaca 660
gactggcaac gatataaatc tcgaatcgtt gcgtatttta ctacttctct tttagctggt 720
ggagcatcca tgattatggt cttacctgct gtcttagatt tacgaaccaa tggtgaaact 780
ctttctgaaa ttacaacctt taaaacagaa gcgactgcct ttttagatat tattatgaaa 840
aatatgattg gcgtttacga tacaaccaaa tatgggtcca ttccttttat ttacattggg 900
ttgttgcctt tgattttttg tctcttctat ttcgttacga aagaagttcc attgaaaaac 960
aaactacttt ttggcagttt gtttgtccta ttgattgcta gcttttacat cacgccctta 1020
aatttattct ggcacggtat gcatgcgccg aacatgtttc ttttccgcta tagtttcctc 1080
ttttcattct tagttatttt gcttgcaggt tatggttggg agaaatttga aaaggatgat 1140
ctcggtgtct taagcggctt gattttgatt cttttagcaa tttttgctct tgcaatgggc 1200
acaaaaggtg ctactagtta tacgtatgtg actttaacct ctttcgtatt aacggcaact 1260
tttttactat tatacttttt cgggattgct ttttatcaat tgaaaaaagc gcctatgcaa 1320
tacttagtga ttttgttatt gctgctcgtt tctggtgaag caatcattaa cacacgggcg 1380
atggtcactg gtattttaga tgattggaac tacgcatcac gcagcttgta ttctgagcct 1440
tatcctgact taaaaaatct agtcgatcaa acaaaaaaag aaaatgatac cttttaccgc 1500
ttggaaaatt taaatggtgt ttcggccaat gacggcatta actatggcta cagtggcatt 1560
agcatgtttt cttccgtacg gaaccgacat tcttcaacat acctaaacgc cttaggtttc 1620
cgctcacgag gcacgaattt aaatattcgt taccaaaata ataccttgtt aatggatgct 1680
ttgatgggca ttaaatacaa tattgctgaa aacaatccaa tgaaattcgg ctttgagcga 1740
caagctgctg ctggtaaata tcagctttat cgtaacgaaa atgctttgcc gttaggattt 1800
ttagcagaca aagagattta caatgtccgg caacctctca atgataattt aggtagtcaa 1860
acaaatttac taaatgcttt agccaatact aatgaacggt attttacctt ttatcaacca 1920
acgatgacgc ttcaaaataa cgtgacaatt acgcaaaata cagcgggcgt aacttttacg 1980
gagaaacaac ataacgtagc caaagagatt tcttatacag tcaatgtgcc cgccaatacg 2040
caagcttatt tgagtttatt cccaacggat tttgctcaat tggaaagttc cactgcgacg 2100
gtcaccgtga acggctcaag tcagcaatca caaattggca ttactggcca atagtataac 2160
ttaggttact atcccaaaga tacaaccgtt aacttcaaag taagttttta tggaaccaaa 2220
gcagtaagtt ttgttcagcc gcaagtggtt ggcttaaata ccaatgcatt tgaaaaagcc 2280
atttccgccg tgcaagaaaa aggcgttgat ttaacaactg gcaaacgctc tgcttcaggt 2340
acctttaccg ccgacaaaga tcaagtactc gtcactacaa ttccttatga caaaggttgg 2400
cgtgtcaaaa ttgatggcaa aaaagttacc ccaaaagcct ttaaagatgc ctttctaagc 2460
gttcctgtca gcgctggaac ccatactatt cagttctctt atttaccaga aggattgatt 2520
cctggcatcg tgttattcgt tctatgtact ggtggtttcg ttgcctacgt aaccttgatt 2580
cctgctagac gaaaccgtaa aaaagaagac aaa 2613
Seq ID 3
gtgaaaaagt tgagctttaa aaaagtgaag tggggcatgc attttttaat ggctgttgcg 60
ttgatagcgc caagtgttac tagtacggca tatgcagtag aaacaacgag tcaacaaagt 120
tcagaagcag taacaagtac caccgattca agtagaaaac aagaaccagt cattacacag 180
gaaacaacag acatcaaaca agaagcacca aatcaggcta cgagtgacag tgtcaagcag 240
tcacaagaaa ccacagcacc aacagagacg acgaatttag aaacgtcaat cgctgaaaaa 300
gaagaaacga gcacgccgca aaaaataaca attttaggta cgtcagatgt tcatggtcaa 360
ttatggaatt ggtcttatga agatgataaa gaactaccag ttggtttgtc ccaagtaagt 420
acagtcgtta accaagtccg ggcacaaaac ccagcaggca ccgttttaat tgataatggc 480
gacaatattc aaggcactat tttaacagat gacttgtata ataaagcgcc tttagtgaat 540
gaaaagaccc atccaatgat caccgccatg aatgtgatga agtatgatgc aatggttttg 600
ggaaatcatg agtttaattt tggtttaccg ttaatcaaaa aaattcaaca agaagccact 660
tttccaatct tgtctgcgaa tacctacaat aaggaagatg gtcttcgttt tgttgaaggg 720
actaccacga aggaacttga ttttaatcaa gatgggcagc cagatttaaa agttgggatt 780
atcggcttaa caattccgca cattcctttg tgggatggcc ctcgtgttac ttcgcttaat 840
tttttacctt tgaaagaaga agcagaaaaa gcagttactg agttgaaagc taacgatcag 900
gctgacatta ttgttgcctc gattcatgcg ggacaacaaa atagtgatcc ggctgccagt 960
gccgaccaag taattgaaaa tgtcgcgggg attgatgcgt atattctggg tcatgaccac 1020
ctttctttta ccaagcaagg agcagcgccg aatggaaaaa ctgtaccggt agggggaccg 1080
aaagatacgg ggacagaagt tgtcaaaatt gatctttcag ttgctaaaaa tgccgataag 1140
tgggaagtgc aagaaggtac agcaacgatt gtaccaacaa cgaatgttcc agcagatgaa 1200
gcagttaagg cagcgacaaa agaataccat gaaaaaacgc gagcgtttat tcaggaggag 1260
atcggcacag caacagctga ttttttacca aaacaagaaa ttaaaggaat tcccgaagca 1320
caattacaac caacagcgat gatttcttta attaataacg ttcaaaaaga agtaacgggc 1380
gcacaattaa gtgcggcagc gctgtttaaa tacgacagta aattacctgc ggggaagatt 1440
tcctatgcca cgatttttga tatctacaaa tacccgaata ccttagtgag tgttcccatt 1500
aacggtgaaa acttactgaa gtatttagaa aaacaagggg cgtactataa ccaaacacag 1560
ccagatgatt tgaccattag ttttaatcca aacattcgtg tatataacta tgacatgatt 1620
tctggagtgg actacaagat tgacatttca aaaccagtgg gtgaacgaat tgtagatgcg 1680
aaaattgacg gccaaccgct ggatcctgcc aaagaatata cgattgctat gaataattat 1740
cgttacggcg gtttagctag ccaagggatt caagtagggg aacctattaa aaattctgat 1800
ccagaaacct tacgaggaat gattgttgat tatattaaga aaaaaggaac tcttgatcca 1860
gaacaagaaa tcgaacgaaa ttggtcaatt attgggacaa attttgatga aaaatggcgt 1920
gccaaagcaa tcgaattagt gaatgacggc actcttcaaa ttccgacttc tcctgatgga 1980
cgtacaccaa acgccgccgc tattacgaaa caagatgtcc gtaatgcggg ctttgattta 2040
gataatgcat ataccattat gcacacaaat gacgttcatg gccgactaga agcagggaaa 2100
ggcgaattag gtatggcgcg tctaaaaacc tttaaagacc aagaaaaccc aaccttgatg 2160
gtggatgcag gggatgtttt ccaaggatta ccaatctcca atttctccaa aggcgcggat 2220
atggccaaag caatgaatga agttggttat gatgccatgg cggtgggaaa tcacgagttt 2280
gattttggtt tagagattgc actaggttat aaagaccaac tgaattttcc gattttatct 2340
agtaatacgt attacaaaga tggcagtgga cgggtttttg atccgtatac aatcgtagaa 2400
aaatccggga aaaagtttgc cattgtaggt gtgacgaccc cagaaacagc aacgaaaaca 2460
cacccgaaaa acgtagagaa ggtgacattt aaagacccga ttccagaagt agaagcagtg 2520
attaaggaaa ttaaagagaa gtacgcggat attcaagctt tcgtggttac tgggcattta 2580
ggcgtagatg aaacgacgcc gcatatctgg cgtggtgata cgctagcaga aacccttagt 2640
caaacatatc ctgagttaga tatcactgtg attgatggac attcgcatac agccgtcgaa 2700
agtggcaaac gttatggcaa agtgatctat gctcaaacag gtaattattt aaataatgtt 2760
gggatcgtca cagcaccaga gagtgaacca actaagaaaa caacaaaatt gatttcagca 2820
gcagagctgc tagaattgcc agaaaacccg gcagttaaag ccatcgttga tgaagcacgt 2880
acgaatttta acgctgaaaa tgaaaaagta attgtcgatt atattccatt cacattggat 2940
ggacaacgag aaaatgtgcg cacacgagag accaacttag ggaatttgat tggtgatgcg 3000
attatgtcat atggccaaga cgcgtttagc caacctgctg attttgcagt aactaatggt 3060
ggcggcattc gcgctgatat taaacaaggg ccaattaaag ttggggatgt cattgctgtg 3120
ttaccttttg gcaatagcat tgcgcaaatt caagtaaccg gcgcccaagt taaagaaatg 3180
tttgaaatgt ctgttcgttc gattccacaa aaagatgaga atggcacaat tttactagat 3240
gatgctggcc aaccaaaact tggcgcaaat ggtggtttcc tacatgtttc aagctccatt 3300
cgtatccact atgattccac aaaaccaggt actcgcttgg ctagtgacga aggcaatgaa 3360
acaggacaaa cgattgtcgg tagtcgcgta ttaggaatag aaattaaaaa tcggcaaaca 3420
caaaagtttg aaccattgga tgagaagaaa caataccgga tggctaccaa tgatttctta 3480
gctgctggtg gtgatggtta cgatatgcta ggtggtgaac gagaagaagg gatttcacta 3540
gattctgtct taattgaata cttgaaaagt gcaaccagct tgcggttgta tcgtgcagca 3600
acgacgattg atttagcaca atataaagaa ccattcccag gcgaacgaat tgtttctatt 3660
tcggaagaag cttacaaaga gttaatcggt ggaggagaga cgccaaaacc agatccaaaa 3720
ccagacccga aaccaacacc agaaacacca gtagcaacca ataaacaaaa ccaagcggga 3780
gcaagacaga gcaatccatc cgtaacagag aagaaaaagt atggcggctt tttacctaaa 3840
acgggtacag aaacagaaac gcttgcatta tatggtttac tgttcgttgg actttcttct 3900
tctggctggt atatttataa acgacgtaac aaagctagt 3939
Seq ID 4
atgaatcaac agactgaagt aaaaaaacgt tttaaaatgt ataaggcaaa gaaacactag 60
attgtagccc ctattctttt tttaggtgta ttaggagctg taggattagc tacagataat 120
gtacaagccg cggaattaga tacgcaacca ggaacaacga cggtgcaacc cgataatccc 180
aacccgcagt cacgaaatga aacacttaaa acggcagtat ctgaagaagc agcattacaa 240
aaagacacta cttctcaacc aaccacagca gaagaagtag tgccgaaagg aattgctgct 300
gaacaaagtt cagctacctc aaatgatacc acaaacgtcc aacaaccaac agcagaagca 360
gaaaaatcag cacaagaaca accagtagtc agccctgaaa caaccattga acctctaggg 420
cagccaacag aagttgcacc agcagaaaat gacgctaata aatcaacgtc aattcctaaa 480
gaatttgaaa caccagacgt tgacaaagca gttgatgaag cgaaaaaaga tccaaacatt 540
accgtcgtgg aaaaaccaac tgaagactta ggaaatgttt cttctaaaga tttagctgca 600
aaagaaaaag aagtagacca actacaaaaa gaacaagccc aaaagattgc ccaacaagca 660
gctgaattaa aagccaaaaa tgaaaaaatt gccaaagaaa atgcagaaat tgcggcaaaa 720
aataaagcgg aaaaagagcg ctacgaaaaa gaagtggcgg aatacaacaa acacaaaaat 780
gacaaaggct atgtgaatga agcaatcagc aaagacttag tttttgattc aagcattgtg 840
actaaggata ctaaaataga caagattaca ggtggcaagt ttattaaagc ttccgatttt 900
aacaaagtaa accaaggaca atccaaagat atttttacaa aattaagtaa ggatatgaat 960
ggaaaagcaa caggcaactt ccaaagctca aaagtggctg ctgttgaatt tggccctaaa 1020
ggaggatacg cggttctttt agaaaaaaac aaaccggtca atgtcacgta tacaggatta 1080
aatgctagtt atttagaccg aaaaatcaca aaagcagagt tcatttatga acttcaatct 1140
gcaccaagtc aaagcggcac cttaaatgca gtattttcga atgatcctat tattacagca 1200
tttgttggta ctaaaaatgc caatgggaag gacgtaaaag ttcgcttaac cattaaatta 1260
tatgatgcta atggcaaaga agttttacca gataaagacc atgcctttgc ctatgcgtta 1320
tcgtcattaa actctagtct aggaacaaat tatagtgtag aacatgcgga atttgtttca 1380
gactttggct caaaaaatga gttcaagtat attaatggtt cctacgtaaa gaaacaagcg 1440
gatgggaaat tctactcaac cgaagacctt gattatggga caggtccttc aggactaaaa 1500
aatagtgatt gggacgctgt aggtcataaa aatgcgtact atggttctgg tgtaggtcta 1560
gctagagagg gtggacgtat ttctttctct tttggtatga caaccaaagg aaaagtcaac 1620
ctatctggtg cgcaatggtt tgcctttagt accaatttaa atgcgaaatc aattagacca 1680
taccaaaaga aagggaatcc aaaagaacca gaaaaagcaa caattgaatt caatcgatac 1740
aaagccaatg tcgttcctgt tcttgtgccg aataaagaag tcactgatgg tcagaaaaat 1800
atcaatgatt taaatgtgaa acgaggcgat tctttacaat acattgtgac aggggatacg 1860
acagaacttg ccaaagtaga tccgaaaaca gtgacaaaac aagggattcg ggataccttt 1920
gatgcagaaa aagtgacgat tgatttatcc aaagtgaagg tttatcaagc agatgcaagt 1980
ctaaacgaga aagacttaaa agctgttgct gcagcgatta attcaggaaa agctatagac 2040
gtgactgctt cttatgttct taatttagat caaaacaccg tcacagcaat gatgaaaacc 2100
aacgcagacg gttccgttgt tttagcaatg gggtataaat atttacttgt cttgccgttt 2160
gtagtgaaaa atgtagaagg cgattttgaa aatacagctg ttcagctgac aaatgatggc 2220
gaaacggtaa caaatacagt gattaaccat gtgccggtta gtaatccttc caaagatgta 2280
aaagcagata aaaacggtac agttggcagt gtttctctac atgataaaga tattccgtta 2340
caaacaaaaa tttattatga agtgaaatct tccgaacgtc cagctaacta tggcggaatt 2400
accgaagaat ggggcatgaa tgatgtcttg gacacgaccc atgatcgttt cacaggtaaa 2460
tggcacgcta ttacaaacta tgaccttaaa gtaggggaca aaacgttaaa agcaggaaca 2520
gatatttctg cctacattct tttagaaaac aaagacaata aagacttgac gtttacaatg 2580
aatcaagcat tattagcagc gttaaatgaa ggaagcaata aagtaggcaa acaagcttgg 2640
tctgtatatc tggaagtcga acggatcaaa acaggtgacg tagaaaatac gcaaacagaa 2700
aactacaaca aagaacttgt tcgttctaat acggtggtga cgcatacacc tgatgatcca 2760
aaaccaacca aagccgttca caacaagaaa ggggaagata ttaaccatgg aaaagttgct 2820
cgtggtgatg ttctttctta tgaaatgacg tgggacttaa aagggtacga taaagacttt 2880
gcttttgaca cagtcgatct tgcgacagga gtttctttct tcgatgatta cgacgaaacg 2940
aaagtgacac caatcaaaga cttacttcgt gtcaaagatt ctaaaggggc agacattacg 3000
aaccagttca cgatctcttg ggacgatgcc aaaggcacgg tgacaatctc tgccaaagac 3060
ccacaagcct ttattctagc gtatggtggg caagaattgc gtgtaacgct ccctacaaaa 3120
gtcaaagcca atgtttctgg tgatgtttat aattcagcgg aacaaaatac atttggtcaa 3180
cgaattaaaa caaataccgt tgtcaaccat attccaaaag tgaaccctaa aaaagacgtg 3240
gttattaaag ttggtgacaa acaaagtcaa aatggcgcca caatcaaatt aggggagaaa 3300
ttcttctatg aatttacaag tagtgacatt cctgcagaat acgctggtgt tgtggaagaa 3360
tggtcgatta gcgataaact agacgtaaaa catgacaaat ttagtggcca atggtctgtg 3420
tttgccaatt ctaattttgt tttagcagac ggaaccaaag tgaataaagg ggacgacagt 3480
tcgaaactat tcacgatgac ctttgaacaa ggggtagtta aaatcacggc cagtcaagcg 3540
tttttggatg cgatgaatct aaaagaaaac aaacacgttg cgcactcatg gaaagcgttc 3600
attggtgtag aacgaattgc ggcaggagac gtttacaaca caatcgaaga atctttcaac 3660
aatgagaaga ttaaaactaa tacggtagtg acacatacgc cagaaaaacc acaaacacca 3720
ccagaaaaaa cagtgattgt accaccaaca ccaaaaacac cacaagcacc agtagagcca 3780
ttagtggtag aaaaagcaag tgtggcaccc gaactacctc atacaggtga aaaagaaaac 3840
accctattat ctgtactagg tgccggaatg ctagttggtc tggcttggtt tggtttgaaa 3900
aaacgtgaag tgaaa 3915
Seq ID 5
atgaaacgaa ttggttatgc acgcacaact attattgaag acgatttaaa aaatcaacta 60
accactctcc aatcgtttgg ttgtgatgat attttccaag agacatttga tcctcaagcg 120
gagatcagtg tcttagacga agtcgaaaaa cttctttctg ctggggatac acttattgtt 180
tgtaaattgc accatttagg taaaacaacc cgccaattaa cagactttat gaaaatgtta 240
aaggaaaaac aagttgattt tgtcagcatt tcagaaggaa ttgatactca ccttccaaca 300
ggtgaagcct atttccaatt aatggagagc ttatctgcga tggaatgtgc actaattaaa 360
gaacgaacac tcgtcgggct ccacaaagct cgtgaaaacg gaaaagtagg tggacgtcca 420
aaaatcgatg gacgcaccgt ccgcaaaatc cgtgcattat attatgaaaa caaagaaaca 480
atccaattta tttctaataa atgcggcgtt tcggtgggca cttgttataa gtacatcaat 540
ttacctgaga cagatgtcga gcggctgtat tcc 573
Seq ID 6
atgagtaaaa aagaaataaa tcaagtagtt gccagtagct atcaattgta tattaatgga 60
gagtggacaa caggtagtgg taacaaaatg attgctagtt acaatcctag taatggcgaa 120
aaattagcag aatttgtaga tgccacaaat gcagatgtgg atcgagctgt agaagcagcc 180
caagaagcgt ttcagacatg gaaagatgtt gacgttgtaa caagaagcaa tcttttgttg 240
aaaattgctg atttgattga agaaaatcaa gaacatttgg ctatggtgga gactttagat 300
aatggaaaac cgcttcggga aacgcaatcg attgatgtcc ctgccagtgc agaccatttt 360
cggtattttg ctagtgtgat tcgtggagaa gaaggatctg tcaaagaatt tgataaggat 420
acgttatcca ttgttgtgaa agaacccatc ggtgttgtgg gtcaaattat tccgtggaat 480
ttccccttat taatgggtgc ttggaaatta gcaccagcgt tggcagcagg taacacggtt 540
gtgattcatc cgtcctcgag cacatcatta agtctgttgg aattgtttaa aatttttgat 600
caagtcttgc cgaaaggagt agtgaattta atcaccggtc gtggttctga ttcaggaaat 660
tatatgttgg cacatccagg gtttgataag ctagctttta caggctcaac agaggtgggg 720
tacactgtcg ctaaagcggc ggccgaccgc ctaattccag ccactttaga acttggcgga 780
aaatcagcca acattatttt tgaagatgcc aattgggaac gtgcattaga aggcgtgcag 840
ttagggattt tattcaatca agggcaagtt tgttgtgctg ggtctcgtgt gttcgttcag 900
tcaggtattt atgatcaatt tgtagaagct ttaaaggaaa agtttgaaca agtgaacgtt 960
ggtttcccgt gggaaaaaga tgttgaaatg ggcgctcaga tcaatgagca tcaattggaa 1020
gaaattttaa aatatgtcga aattggtgtg aaggaaggag ctacgctgat tactggtggg 1080
caacgtttaa cagaaaatgg gctagacaag ggggcgtttt tagcacctac gttattagcg 1140
aatggtacga atgcaatgtg tgtggcccaa gaagaaatct ttggtcctgt tgcaacagtg 1200
attaaatttg aaacggaaga agaagtcatt cgtttagcta atgattctga atatggtcta 1260
ggtggtgccg tcttttctca agatatcaat gtggcattac gggttgctcg tggtgtacgg 1320
acaggtcgaa tgtgggtcaa cacatacaat caattgcctg cgggcgcgcc atttggcggt 1380
tataaaaaat caggaattgg tcgagaaacg cataaatcaa tgctagatgc ttatacgcaa 1440
atgaaaaata tttacattgt gacaaaagaa gaagcagatg gactgtat 1488
Seq ID 7
atgggaaaat atcatgaact agcagaaaaa attgtaaaaa atgtcggtgg gcaagagaat 60
attaatagtt tgacacattg tatcacacgt cttcgcttca aattaaaaga tgagatgcaa 120
gcgaatgatg atgtcttaaa aaatatggac ggtgtggtaa ctgttatgaa aagcggtgga 180
caataccaag tcgttattgg gaaccatgtt ccagccgttt acgaagaagt agttagtatt 240
gcaggcttgt caggagaacg tgaagaagaa gcttccagtg ggaatttatt tgatcgctta 300
atagatattt taagtggttg tttccaaccc tttttagggg cgttagcagc ggctgggatg 360
gtcaaagggt taaatgcctt attggttttc ttaaagctct atacggctac gtcagggact 420
tataccatgt taaatgggat tggcgatgca attttctact ttatgccagt tattttaggt 480
tatacagcag ctaaaaaatt ccggttgcat ccaatggtag ggatcgtgat tggcgcagct 540
ttatgttacc caacgattca aggaagtgca ttacaaacgg cctttgaaac aacagcgggt 600
gccggagccg cagcaccgta taatttattc ggtttacctg cctataatac atttatgggg 660
attccttggg tcggtgcgaa ctatacaagt agtgtcgtac caatcatctt cattattgct 720
tttgcggcac aagttcaaaa agtgtttaaa cgtattattc cagaagttgt tcaaacgttc 780
ttagtaccgt ttttcgtctt gttgattgcc ttaccaattg gtttcttagt aattgggcca 840
atcgttagca tgctaacaga tttattaagc gctggcttta cagcattaat gagtttctca 900
ccagctttgt atggtttgat tcttggtttc ttctggcaag tcttagttat ttttggttta 960
cattggagcg ttgtgccatt agctattatg caagtgacac aagaaggttc aagccaagta 1020
ttaacgggat catttgcagc tagttttgcg caaacagcgg ttgttttagc gatgttcttc 1080
aaattgaaag ataaaaagtt aaaagctttg tgtccgccag caattatctc tggtattttt 1140
ggtgtaacag agccagcaat ttatggaatc actttaccaa aaaaatggcc atttatttat 1200
tcaatgattg gtggcgctgt cggtggttta tatttgatga tcaacaatgt gacagcttat 1260
acaatgggtg gtttaggaat ttttggtgtg ctaaacttta tcaatggtga tgatgcaagt 1320
gggatgatcc agtcctttat tgcaattgcc attgcagcgg tcgttggttt tggtctaaca 1380
ttcttcttct ggaaagacaa tacagttgag gaagaagaag tgattattga taaaacaacg 1440
attaaaaaag aaaatattac aagtccagtc aaaggacgag tgttatcttt aaaaaatgca 1500
gaggatcctg cttttgcaaa tggagcatta ggaaatgggg tcgtgattga accaacggaa 1560
ggtaaagtgg ttgcaccctt tgatggaacg attgtcacac tattcccaac aaaacatgca 1620
ttaggtttga tttcagataa cggcacagaa ttattgattc atattgggat tgatacagtt 1680
caattagaag gcgaaggctt tgaagctttt gtaaaacaag gtgaccgcgt gaaaaaaggt 1740
caaacattag taacctttga tttagaagga attaaaaaag ctgggtttag tacacaaatt 1800
ccaatcgttg tgacgaatac agcggattat ttagatattt tggaagtcgg cagtaatgaa 1860
gtttcaacaa gtgatgattt gctgaccgct ttaata 1896
Seq ID 8
atggaaagta aaacgtttga tattgaaggg atgagctgtg cttcctgtgc ccaaacgatt 60
gaaaaggcta cggcgaagtt acctgggatg gcaaaggcaa gtgtcaattt agctacggaa 120
aaattaagtg tcacgtatga tcagacggaa gtcacagaag aagaaattaa ggaagctgtg 180
tcagatgctg gctacaaggc gattagccca gcccagcaac gaacgtttgc gattgaagga 240
atgagttgtg cttcctgtgc ccaaacgatt gaaaaagctg tgaaccaatt atctggtgtt 300
caacaagcga ttgttaattt agccacggaa aaactggtgg ttagttatga cgaccatcaa 360
gtaacgagtg ctgaaattat caaagcagtt acggatgcag gttatcaagc gacagaagaa 420
gtcgctgcag gtgcaacagc ggaccaagat cgtgaaaaga aacaaaaaca tattgctgag 480
atgtggcaac gtttttggat ctcagcggtg tttacagtgc cccttcttta tattgcgatg 540
ggacatatgg taggcttacc attgccggat tttttgaatc caatgacaca tgcgatgacc 600
tttgcaatgg ttcaattgat tttgacttta cccgttctat atgtcggcag agaatttttc 660
acagttggtt ttaaagcatt gtttaaaggg catccgaata tgttttcttt ggtggcttta 720
ggaaccagcg ctgcttttgt gtatagttta tatggcacag tcatgatatt tttaggagac 780
acaagtttta caatggctct ttattatgaa tcagcgggtg ttattttaac cttaattaca 840
ttaggaaaat atttcgaagc tgtttcaaaa gggaaaacct cagatgcgat aaaaaaatta 900
atgggtttag cgccgaaaac tgctcatatt ttacgtgatg gcgtagaaat tgaagtgcca 960
gttgatgctg ttcagttgga tgacattgtg attgttcgcc caggagataa aatccctgtt 1020
gatggagtga ttgtttcagg cagtagttct gtggatgaag ccatgttaac gggcgaaagt 1080
ttgcctgttg aaaagaaagt cggtgatgca gtaattggtg ctagcatcaa taaaaatggc 1140
agctttcaat tcaaggcgac aaaagtggga aaagagacgg cccttgccca aatcattcaa 1200
ttagtggaag atgcgcaagg ctcaaaagcg ccgattgcac aattagctga taaaatttcg 1260
ggtgtttttg tcccaatcgt cattggtttg gctgttcttt ctggcttggc ttggttcttt 1320
ttaggacaag agtcatggat tttcgctttg acaattacta tttctgtgtt agtgattgct 1380
tgtccttgtg cgctcggctt ggcaacgccg actgcgatta tggtggggac aggtaagggc 1440
gctgaaaatg gcgttttaat taaaagtggg gatgctttag aaaccactca caaaattcaa 1500
acaatcgtgt ttgataaaac agggactatt acagaaggca aaccggtggt tacagatatt 1560
ttagttgctg attcagcact ttcagaagct gagttactaa cattagcagc ttctgcagaa 1620
caaggatcag aacatcctct aggtgaagca attgtgggag cggctaaaga acgtcaattg 1680
ccattagcag aaggaagcga tttttcagcg attccaggtc acggcatccg tgtgacagtc 1740
aacgaacgag tcctactttt gggcaatata aaattaatga aagaagaagc aatcgagcta 1800
tcaacgtttg ttcaacaagc ggatcgtttg gcagaagaag ggaaaacccc aatgtttgtg 1860
gcaaaagatg gttcgtttgc gggtatcatt gcggtagcag atactgtgaa ggatagcagt 1920
caaacagcca ttgcacgttt gcataaaatg gggatcgaag ccgtaatgat tacgggagac 1980
aacaagcgta ctgcagaggc gattgcgaaa caagtaggta tcgatcgtgt cttaagtgaa 2040
gttttaccag aagataaagc gttagaagtg aaaaaactac aagcagaagg caaaaaagtt 2100
gcgatggtgg gtgacgggat taatgatgca ccagcacttg cccaagcaga tgtggggatt 2160
gctattggtt caggcacaga tgttgcaatg gaatcggccg acattgtgct aatgcgtagt 2220
gatttaatgg atgtgcctac agcagttgaa ttaagtaaag cgaccatcaa aaacatcaaa 2280
gaaaatcttt tctgggcatt tgcctataat acattaggta ttccagtggc tatgggtgtg 2340
ttacacttat ttggtggacc tttgttaagt ccgatgattg cagcagcagc tatgagtttt 2400
agttcagtct ctgtcttact aaacgcctta cgattgaaag gatttaaacc ttcgacggtg 2460
aaaagaacta gtggaagtca aaaa 2484
Seq ID 9
atgaaaaaga aaattttagc aggagcgctt gtcgctctgt tttttatgcc tacagctatg 60
tttgccgcaa aaggagacca aggtgtggat tgggcgattt atcaaggtga acaaggtcgc 120
tttggctatg cacatgataa attcgctatt gcccagattg gaggctacaa tgctagcggt 180
atttatgaac aatacacata taaaacgcaa gtggcaagtg ctattgccca aggtaaacgt 240
gcgcatacct atatttggta tgacacttgg ggaaacatgg acattgcgaa aacaacaatg 300
gattactttt tgccacgtat tcaaacgcct aaaaattcca tcgttgcatt agattttgaa 360
catggagcgt tggctagtgt tccagatgga tatggaggat atgtaagttc agatgccgaa 420
aaagcagcaa atacagagac aattttgtac ggtatgcgca gaatcaaaca ggctggctat 480
actccaatgt attacagcta taagccattt acactaaatc atgtaaacta tcaacaaatc 540
atcaaagagt ttcctaactc tttatggatt gctgcgtatc ctatcgatgg tgtgtcacca 600
tatccattgt atgcttattt cccaagcatg gatggtattg gtatttggca attcacatcc 660
gcttatattg caggtggttt agatggtaac gtagatttaa caggaattac ggatagtggt 720
tatacagata ccaataaacc agaaacggat acgccagcaa cagatgcagg cgaagaaatt 780
gaaaaaatac ctaattctga tgttaaagtt ggcgataccg tcaaagtgaa atttaatgta 840
gatgcttggg caactgggga agctattccg caatgggtaa aaggaaacag ctacaaagtg 900
caagaagtaa ctggaagcag agtattgctt gaaggtatct tgtcatggat tagcaaaggt 960
gatattgaat tattgccaga cgcaacagtc gtccctgata agcaaccaga agcgactcat 1020
gtggtacaat acggagaaac attatcaagt attgcttatc aatatggaac agactatcaa 1080
acgttggcgg cattaaatgg attggctaat ccaaatctta tttatcctgg tcaagttttg 1140
aaagtcaatg gatcggcaac aagtaatgtc tacacggtta aatacggcga taatttatct 1200
agtattgcag caaaacttgg cactacttat caagctttag ctgcattaaa cggattagca 1260
aatcctaact tgatttatcc aggtcaaaca ttgaattat 1299
Seq ID 10
ttgccgatga ttattaaaca ggaacaattc ataccaaaag atacagtaga aacaacgatt 60
gacttattaa ttagaaattt aacaacgatt aaagataaca caggtgaatt tctgttagat 120
tttgatggtt taaaagttga tgacaaaagt tggacgattt ggaactggcc gcaaggcgtt 180
gggttgtatg gtatatataa gaattatcga aatactaaaa gtgaaaaagc tttacaagta 240
gttaacgact ggtttgaagg tcggatgcaa gaaggcgcgc cgcctaagaa tgtgaatacg 300
atggctccgc ttttaacaat ggcttatcta tatgaagaca ctaaagactc taaatatata 360
ccttacttag agcaatgggc ggaatgggtg atggaagaaa tgccccgaac gaatgaaggt 420
ggcttgcaac atgcgacata tggaccagaa aataaaaatc aattatggga tgatacgtta 480
atgatgactg tcttaccttt ggcaaaaatt ggtaagttgt tgaatcgact cgattatttg 540
gaagaagcaa agcatcaatt tttaatccat attaaatatt tacaagataa aaaaagtggt 600
ctatggtatc acggttggac ttttgaggga aatcataact atgcggaagc actgtgggca 660
agaggaaact gctggattac gattgctatt ccagaaatta ttgaaatctt agagttgcca 720
aaaggagata gtttacggga atttttattg agtaccctca atgcgcaagt ggcagcttta 780
gcgaaatacc aagatgaatc tggtttatgg catacattaa ttaatgattc aaattcgtat 840
ttagaatctt ctgctacagc gggattcgct tatgggattt taaaagcggt tcataaaaaa 900
tatatttctt ctgaatatga agaagtggca aacaaagcaa ttgctggctt actaaatgag 960
attgatgaaa cgggagaagt acaacatgtg tcagttggta caggaatggg tgataattta 1020
gatttttatc gcacaattgg aatgacagcg atgccttatg gtcaatcatt aacaatccta 1080
tgtttgactg aattgcttgt ttcttattgc 1110
Seq ID 11
atgaaagcaa aqaaacaata taagacatat aaagctaaga atcactgggt aactgtccct 60
attctttttc taagtgtgtt aggagccgta ggattagcta ctgataatgt acaagccgcg 120
gaattagata cgcaaccaga aacaacgacg gttcaaccca ataaccccga cctgcagtca 180
gaaaaggaaa cacctaaaac ggcagtatct gaagaagcaa cagtacaaaa agacactact 240
tctcaaccga ccaaagtaga agaagtagcg ccagaaaata aaggtactga acaaagttca 300
gctaccccaa atgataccac aaacgcgcaa caaccaacag taggagctga aaaatcagca 360
caagaacaac cagtagtaag ccctgaaaca accaatgaac ctctagggca gccaacagaa 420
gttgcaccag ctgaaaatga agtgaataaa tcaacgtcca ttcctaaaga atttgaaaca 480
ccagacgttg ataaagcagt tgatgaagta aaaaaagatc caaacattac cgttgttgaa 540
aaaccagcag aagacttagg caacgtttct tctaaagatt tagctgcaaa agaaaaagaa 600
gtagaccaac tacaaaaaga acaagcgaaa aagattgccc aacaagcagc tgaattaaaa 660
gccaaaaatg aaaaaattgc caaagaaaat gcagaaattg cggcaaaaaa caaagcagaa 720
aaagagcgtt atgaaaaaga agtcgctgaa tacaacaagc ataagaacga aaacagctat 780
gtcaatgaag cgattagtaa aaacctagtg ttcgatcaat ctgtcgtgac gaaagacact 840
aaaatttcgt cgattaaagg cggaaaattt atcaaagcaa ctgattttaa taaagtaaat 900
gcaggggatt caaaagatat ctttacaaaa ttacggaaag atatgggcgg gaaagttact 960
ggcaacttcc agaattcctt tgtaaaagag gcaaatcttg ggtctaatgg tgggtatgcg 1020
gttcttttag aaaaaaataa accagtgaca gtgacctata caggactaaa cgctagttat 1080
ttaggacgta aaattacaaa agcagaattt gtttatgaac tacaatcctc accaagccaa 1140
agtggaacgt taaatgcagt attttcaaac gatccgatta tcacggcttt tattggtaca 1200
aacagagtca atggtaagga tgttaaaaca cgcttaacga ttaagttctt tgatgcgtca 1260
ggtaaagaag tactaccaga taaagatagt ccatttgcgt atgcgctgtc ttctttaaat 1320
tcaagtttaa cgaataaagg tggccatgcg gaatttgttt ctgattttgg ggcgaacaat 1380
gcgttcaaat acattaatgg ttcgtatgtg aaaaaacaag cggatggaaa attttactca 1440
ccggaagata ttgactatgg cacaggacct tctggattga aaaatagtga ttgggacgct 1500
gtaggtcaca agaatgccta ctttggttca ggtgtaggtc tagccaatgg gcgtatttcc 1560
ttttcttttg gtatgacaac aaaaggaaaa agtaatgtgc ctgtatctag tgcgcaatgg 1620
tttgccttta gtactaactt aaatgcgcaa tcagtgaagc ctattttcaa ttatgggaat 1680
ccaaaagaac cagaaaaagc aacgattgaa ttcaatcgat acaaagccaa tgtcgttcct 1740
gtacttgtgc caaataaaga agtcactgat ggccagaaaa atgtcaatga tttaaatgtg 1800
aagcgtggcg attctttaca atacattgtg acaggggata cgacagaact tgccaaagta 1860
gatccaaaaa cagtaaccaa acaagggatt cgagatacct ttgatgcaga aaaagtgacg 1920
attgatttat ccaaagtgaa agtttatcaa gcagacgcaa gtctaaacga gaaagactca 1980
aaagctgttg ctgcagcaat taattcagga agagctaaag acgtgactgc ttcttatgat 2040
cttcatttag atcaaaacac cgtcacagca atgatgaaaa ccaacgcaga cggttccatt 2100
gttttagcaa tggggtataa atatttactt gtcttgccgt ttgtagtgaa aaatgtggaa 2160
ggcgattttg aaaatacagc tgttcagctg acaaacgatg gtgaaacggt aacaaataca 2220
gtgattaacc atgtgccagg tagtaatcct tccaaagatg taaaagcaga taaaaacggt 2280
acagttggca gtgtttcgct acatgacaaa gatattccgt tacagacaaa aatttattat 2340
gaagtgaaat cttccgaacg tccagctaac tatggcggaa ttaccaaaga atggggcatg 2400
aatgatgtct tggacacgac ccatgatcgt ttcacaggga aatggcacgc tattacgaat 2460
tatgacctta aagtagggga taaaacgtta aaagcaggaa cagatatttc tgcctacatt 2520
cttttagaaa acaaagacaa taaagactta acgtttacga tgaatcaagc actattggcc 2580
gccttaaatg aaggaagcaa taaagtaggc aaacaagctt ggtctgtgta tctggaagtc 2640
gaacggatta aaacaggtga cgtagaaaac acgcaaacag aaaactacaa caaagagctg 2700
atgcgttcta ataccgtggt gacgcatacg cctgatgatc caaaaccaac caaagccgtt 2760
cataacaaga aaggggaaga tattaaccat ggaaaagttg ctcgtggtga tgttctttct 2820
tatgaaatga cctgggactt aaaagggtac gataaagact ttgcctttga tacagtcgat 2880
cttgcgacag gcgtttcttt cttcgatgat tacgatgaaa cgaaggtgac accaatcaaa 2940
gacttacttc gtgtcaaaga ttctaaaggg gtagacatta cgaaccagtt cacgatctca 3000
tgggacgatg ccaaaggcac ggtgacaatc tctgccaaag acccacaagc ctttattcta 3060
gcgcatggtg ggcaagaatt gcgtgtaacg ctccctacaa aagtcaaagc cgatgtttct 3120
ggggatattt ataattcagc ggaacaaaat acatttggcc aacgaattaa aaccaatacc 3180
gttgtcaacc atattccaaa agtgaaccct aaaaaagacg tggttattaa agtcggtgac 3240
aaacaaagcc aaaatggcgc cacaatcaaa ttaggggaga aattcttcta tgaattcaca 3300
agtagtgaca ttcctgcaga atacgctggt gttgtggaag aatggtcgat tagcgataaa 3360
ctagacgtca aacatgacaa atttagtggc caatggtctg tgtttgccaa ttctaatttt 3420
gttttagcag acggaaccaa agtgaataaa ggggacgaca tttcgaaact attcacgatg 3480
acctttgaac aaggggtagt gaaaatcacg gccagtcaag cctttttgga tgcgatgaat 3540
ctaaaagaaa acaaaaacgt tgcgcactca tggaaagcgt tcattggtgt agaacgaatt 3600
gcggcaggag acgtttacaa cacgattgaa gaatctttca ataatgaaac gatcaagact 3660
aatacggtag tgacgcatac gccagaaaaa ccacaaacac caccagaaaa aacagtgatc 3720
gtgccaccaa caccaaaaac accacaagca ccagtagagc cattagtggt agaaaaggca 3780
agtgtagagc cagaattgcc acaaacaggc gaaaaacaaa atgtcttatt aacggtagct 3840
ggaagtttag ctgcaatgct tggcttagca ggcttaggct ttaaacgtag aaaagaaaca 3900
aaa 3903
Seq ID 12
atgaaaaaaa ttctttttgc tagtttattt agtgccacac tactatttgg gggaagtgaa 60
atttctgctt ttgcacaaga aattatccct gatgatacta cgacaccgcc cattgaagta 120
ccaacagaac caagtacacc agaaaagcca acagatccaa caccgccaat tgagccacct 180
gtagaccctg tagagccacc tattacacca acggagccaa cagaaccgac agagccgaca 240
acaccaacag aacctacaac tcctacagag ccaagtgaac cagaacaacc aacggagcca 300
agtaaaccag tagaacctga aaaaccagtt acaccaagca aaccagcaga acccgaaaaa 360
actgtgacac caactaaacc aacagaatct gaaaaaccag tacaaccagc agaaccaagc 420
aagccaatcg acgttgttgt aacgccaaca ggggaattaa atcacgctgg aaatggtaca 480
caacagccaa cagtccctat tgaaacaagt aatttggcag aaatcacgca cgtgcctagt 540
gtaacaacac ctattacaac tacagacgga gaaaacattg tagctgtaga aaaaggtgtt 600
ccacttacac aaacagcaga agggttaaaa cctattcaat caagttacaa agtattgcct 660
agcggaaatg tagaagtaaa aggtaaggac ggtaaaatga aggttttacc atacacaggt 720
gaagaaatga atatcttttt atctgccgta ggcggtatct tgtctgtagt atctgggttt 780
gtcatcttta aaaaacgcaa agctaaagta 810
Seq ID 13
atgggttccc ggttcggact gatgttgaaa caaggtggta acgatgtttt gctaatcgac 60
ggctggcagg agcatatcaa tgccatcaaa gaaaacggat tgaaggctaa ttataatggc 120
gaagaaatta ctgttaaagt ctcaattgtt aatcagaatg aggtgcccac tggtgagcaa 180
tttgatttga ttatcttatt cacgaaggca atgcagttgg aaaaaatgct gcaggatgtt 240
aaaccattaa ttgctgatca cacagaagtt ttgtgtcttt taaacggtat cggtcatgaa 300
gacgtaatcg aaaaattcgt accgatggaa aaaatcttta tcggcaacac catgtggact 360
gctggtctag aaggtcccgg caaggctaaa ttatttggca gcgggtctgt tgaattgcaa 420
aatctaggta ttggccaaga agaatcagcg aaaaaattgg cggaaacttt gtccgcgtct 480
ggtttaaatg ccaaatattc cgacaacatt cattattcaa tttatcgtaa agcttgtgtc 540
aatggtacga tgaatggctt atgcacgatt ttagacgtca atatggcggg gcttggtgca 600
accaaacctg ctcatgacat ggtagtgact attgttaacg aatttgcagc tgttgctgcc 660
aaagaaaacg tcaacttaga cattcccgaa gtaatcgaac atgtagaaac ttgttttgac 720
ccgactacga ttggtatgca tttcccatcg atgcaccaag acttgataaa aaataaccgt 780
ttgaccgaga ttgactacat caacggagcc atttctcgta aaggtaaaaa atatggagta 840
gtaactcctt actgtgattt cttaacgcaa ctggttcata gtaaagaaga aattctagga 900
gcgaaa 906
Seq ID 14
atgcagacaa cagaagaaat gcaatctggc gatggacgcc gaggtcgatt gcggatattt 60
tttggatttg cggctggtgt tgggaaaacc tacggtatgt tgacagaggc tcatgaatta 120
ttgatgatgg gaaaacatgt tgttgtcggc tatatcgaac ctcatgatcg tccagatacc 180
aatcgattat tagaaggatt accgcaaatt cctcctaaga atatttttta taaacaaatg 240
gttttaacag agccggatat tgatcaaatc attcaacaga agcccgaaat cgtgttgatt 300
gatgaactgg ctcattccaa tgcggaaggt tctcgaaatc gtaaacgata ccaggatatt 360
gatgaactgt tgaatgcagg aatcgatgtt tttactacgg tcaatgttca acatatcgaa 420
agtttgaatg atatcgttga agaagttacc ggaattgagg tcaaggaaac ggtgccggac 480
acttttctac ggcaagcgac aataagagtg attgatgtgg agcccgatga gctgatcgaa 540
cgacttgaac aaggaaaaat atatgcgaat gaaaacgcga aacgtgcctt gaaaaatttt 600
tttattccac aaaaattgga ccaattacgt ggattagcaa ttcagcgagc gtcggatcat 660
attaatcgaa ttagtggaaa gacaattggg atacaaagta aattattgac ggtggtgaac 720
gatgcctttc caaaaatgac cgaaaaatgt attcgctgga cagcacgttt agcgcaaggt 780
ctagttgtcg attggacagt gattcaagtt cgtacgcaag aaaatacacc aacgaacatt 840
cctttagccg ataaattagg tgcggaggtt attagtattg aagaggatga tagttttgag 900
acgattgtcg agttcgcaaa aatgacgggt gttactgata ttattatggg gaaaaatcta 960
aggcagccgt ggtatgaaaa aatctttatt gaagcgtttg atgatcgctt gttaaaacga 1020
ttgaaagata cggaacttca tttaattcct ttcaatgaag aaaagcggtc tttatttttt 1080
aagactcgta aagtcattga gggtggcgga aaagatttag tgattgcaat tggtggtgtt 1140
tttctagcga cggtcgtgac ggaattgatg caatatatcc acgttggcga tcaaaatctg 1200
atgcttattt atatttcatt cgtattatta gtcgctcgaa cgacatccgg ttatttttgg 1260
agctcattat cctctatttt aagtgttttg tcttttaact ggttttttgt cgaaccatta 1320
tattctctga cagtatataa acagggatat ccttttactt tattgctcat gctagttgtt 1380
gccctgatga gcagtaattt aatgattcga ttaaagaaac aagcagatac ttctatgaaa 1440
aaagagcacc aaatggaaat tttgtacgaa ttgaacaaac gatatgtttt agtggaaagt 1500
cgaaaacaaa ttcttgatat ttctgccact tatttatcaa gattattgga acgagaagtg 1560
attatttttg atcgtcaagt gaagacagag agtgttcatt gtatcaacga aaaaaaatca 1620
atattaaata atgaagacga agcagctgta gccttttggg cagcaaagaa ccaaaaagaa 1680
gctgggaatg gtacagacac actaaatgga gctaaaggat tctatttgcc gattgctgca 1740
ggcaggaaga cgttggcggt attaggcatt gaacgaaatg cagatcttga tttggaaaat 1800
gatcagctca attacttaaa attggtgctg actcaaattg cggtcatctt ggaacaaaca 1860
gaattgaaag atgagaaaga gcaagttgag ttagaaaatg aacgggaaaa agtccgcagc 1920
aatttattac gagccgtatc tcatgatctg agaacaccat taactgttat ctcagggatt 1980
gctgaaacat taggcattgg aaatgattta aaagaagaga cacagcgaaa attattaaag 2040
gatattcaag aagagtcaca atggctgatt cggatggttg aaaatctttt atccatcacg 2100
cgaatcaaca tggatactat gaaggttaat aaaactgctg aaccagtaga agaggtcatc 2160
gaggctgttt ataagcattt gaggaaggtc tatccagacg gccaagtgga tattcacctg 2220
ccagaagagg tcatttttat tcaagcggac cccattttga ttgaacaagc gttgtttaat 2280
ctgatagaaa atgcgtttcg tcatggagaa aatgatctac cagtcaaact aaacgtctat 2340
caggaaaaag aacaaacagt ttttgaaatt gaaaatcacg gcgaaattcc attaaaacaa 2400
tttcaaaaga tccaatcaaa cttgtccggc acaaatgaag taccggttga ctcaaaaaat 2460
ggtttaggaa tcggattgag tattgtaaaa acaatcgtcc atgcgcacaa tggtaaaatg 2520
gaaatgacga taggtagggg caaaacattg gttcgaattt atttgaag 2568
Seq ID 15
atgaaaaatt tttataaaaa aaaatttgct ttaactgatc agggcgcaga agctttaact 60
aaagcttcta tttctagttt ttttgtatac tgcattaata tggtaccagc attcattata 120
atgatgctga tagacgaatt agttttagaa aatgcaaagc cccgttggct ttattttgca 180
gtatcgtttg ttactttgct tttcatgtat tggttgttag atagggagta tgaaaactta 240
tataatagta cttataaaga aagtgcgcat ttaagagtgc aaattgcaga cgatttgtca 300
aatttaccat tatcctattt ttcaaaacat aatttatcag atttatctca aactatcatg 360
tctgacgttg aaggtattga gcatgcgatg agtcatgcaa tacctaaatc cggcggtatg 420
gctctgtttt tcccttttat ttcagtgatg cttttggttg gtaatgtcaa aatgggatta 480
gctgttattt tgccaacgtt atttagtttt gtcttaatct tgttatcaaa gaaatcccaa 540
acgaaagcca atactaaata ttacgatact ttgagagaaa actcggaaga atttcaagaa 600
actattgaac tgcagcaaga gattaatagc tttaatctat ctaaaaaagt tcaagacaga 660
cttttcaaaa aaatggaaga gagtgaaagg attcatttaa aggtagaatt aagtactttt 720
tcagtcatgg ccttatcctc tattttctca tatgttagtt tagcagtagt cattctagta 780
ggtgttcact tactgttaac gggtgaagtg actatactct acgtcgttgg ttacttacta 840
gccgcaataa aaataaagga ttccttcgat tctatgaaag aagcagtgct tgaaatattt 900
tatttagctc ctaaaataca gcgaattaga gctatgaagg aaaccagtat ccaagaggga 960
tcagatagtc ctctgaaatc ttttgacgtt gagttgcgag atgtctcttt ctcatatgat 1020
aacaataccc caattttaga tcatatctct tttacggcta aacaaggaga agtgactgcg 1080
ttagtaggtg ctagtggttc cgggaaaact agtatcttga aattagtgtc tagactgtat 1140
gattatgatg aaggctgtat tttaattgat gggtatgata ttaaacgtgt atctccagct 1200
tctttgttta gcaaaatcgc tattgttttt caagaggtaa ctttgtttaa tacaagtatt 1260
ttggaaaata tacgtattgg aaatagtcaa gcgtcagatg aggaagtgaa aaaagcagct 1320
cgtttagcta actgcgaaga ctttattgaa aaactacctg acggttatca tacattggtc 1380
ggagaaaacg gaagttcact ttctggagga gagcgacaac gcttatccat agcacgggct 1440
tttttgaaaa atgcgccaat tttaatttta gatgaaatta ctgccagttt agatgcggaa 1500
aatgaaaaac gtattcagga aagtttaaat cgtttaatac aagataagac agtactaatt 1560
atatcgcatc gcttgaagtc tattgaaaag gtaaataaaa ttgtagtgat ggatcaagga 1620
aaagtagttg atcaaggtac tcatagtgag ttgtatcgaa ggtcagaaat atacaaaaat 1680
ctcataaaga aaactaaact atctgagaaa ttcgtctatg aaaaagaagc tcaatcacga 1740
Seq ID 16
ttgactcttt ccgacacgtt aggtcaacga gaagtaacgg ctctttctta cgattcgaga 60
gacgtaacag cagaaacact ctttttttgt aaaggattaa actttaaaga aatctattta 120
gaaaatgctg taaaagacgg cctagaaatc tatgtttctg aggtgcctta tgaggttccg 180
gcgcaactag gaattatcgt gactgacatt aaaaaagcaa tggctgtgtt aagcatggct 240
ttttatgatt atccacaaaa taaattaaaa ctgattggct ttactggaac caagggtaaa 300
acaacagcgg cttatttcac caaatatatt ttagatgttg ctacacaaca aaaaacagcc 360
ttgctttcta ccatgaattc gacattagat ggcaaaacat tctttaaatc tgccttgact 420
acacccgaat ctttggactt gtatcgaatg atggcaaccg ctgttgctaa cgggatgact 480
cattttatta tggaagtttc ttcccaagcc tacaaaacta atcgtgtcta caagttattt 540
tttgatgtcg gcattttttt aaacattacc cccgaccata tcagtccaat tgaacatcca 600
acctttgacg attactttta ttgcaaacgt caattgatta cacattctaa agtcattgtg 660
ttgaatcacg aagctgatta ttttccatta cttaaagaaa ctgcacaaca gcaaaaagtc 720
ccagccattg tctatggcag tcagccggct ccagaagtag actattcatt cgctgtttct 780
tcagaggatt ccttacgttt tatcgttgaa agtcctgcag atgcgttagg attggccggc 840
tcttatcacc ttcgtttagg tggtgatttt aacaaaggta atgcgttaag tgcggcgatt 900
gctagtgtcc ttgtgggagc ttcaaaagaa gagtgtcaac aaggaatcgc agcaaccacc 960
gtccctggcc ggatggaatc tttaacgaat accaacggtg caaccgtcta tgttgattac 1020
gctcataact atgacagttt gaagaatctt ttaacctttg tccgtgaaga acatcctgac 1080
ggccgcttaa ttgttcttgt cggtagcacg ggagacaaag cgatttctcg ccgcaaagac 1140
tttggtaggg ttctttctga attggctgat gttgcagtcc taacaaccga tgacccagcc 1200
agtgaagatc cagcaaaaat ttgccaagaa attcaagccc atattaccaa agaaatgcct 1260
gtttacaccg ttttagatcg tggcgaagca attgctcacg cattatcttt aagcacaacg 1320
gctgatgatg cgattgtctt agctggtaaa ggcgccgacc tttatcaaaa agtaaacggc 1380
gtagatgaac cttacgcagg agactttgcc cttgcggaag ctttcattaa taaaaagaac 1440
Seq ID 17
atgtttttag gatttaatga aatgaagtat tcaaaaggac ggtacgtcct tgtagtctta 60
gtaatggtct tgattgcttg gttaattttt attttgtcag gtttagcgaa cggtttagct 120
caaggaaatc gtttagccgt agatcaatgg caagcgaatc aagtggtttt atcaaaagag 180
gccaatagta atttaaatgt atcagtgtta gatgaaaacg tgaaagaaac gatttcagga 240
ggcaaaattg caccgattgg tcaacaatct ttagccatcc gcccagcaga tgataaaaag 300
gctgaattaa cgaatgttag cttatttggg attgaaaaag aaagtttttt aatgccaaaa 360
gtgattgaag gaaacgcatt tactgataaa aatcaagtga ttgcttcaga aacattgaaa 420
aatcaaggat ttaaaattgg cgacaaatta actgcaggga aatatgatga gcaattagaa 480
attgtcggct ttatttctaa aagtagctat aacattgtgc cagttattta cacttctctg 540
gatacttggc ggtcaattaa atatggtgac aatccagcaa tggccaaaat ggttaatggt 600
ttcattgttc gcagcaagga caacacggaa gttaaaacga ctaataaaga cagccaagtt 660
ctttcaattt cagattttat tgaaaaactg ccaggataca gcgctcaaaa cttgaccttg 720
gatggcatga tttatttctt gattgtgatt gcagcgttca ttatcggtat ttttatcttt 780
gtgatgactt tacagaaaac agcaatgttc ggcgtcttaa aagttcaagg ggtgccaact 840
agtttcttag caaaggcagt catgctacaa accgctttat tggctgttct aggagcggcg 900
attggtcttg ctttaacggg aattaccgtg ctctttttac cagaagcgat gccttatgca 960
actaatggac cacgcatgat tttgtttagt gtactattga ttttatctgc attaattggc 1020
ggggcatttt caattcgaac gattgctaaa attgatccgt taatcgcgat tggaggt 1077
Seq ID 18
atgcaaattt ttccctattc agatgatcca aataaacgct atcacacttg gaactatgca 60
cttcgtcaac agtttggtga aaagattttt aaagtgccaa ttgatggtgg ctttgattgc 120
cccaatcgtg atgggacggt tgccaaaggt ggttgtacgt tctgtagtgt ttctggctca 180
ggcgacatga ttgttgcacc tagcgatccc ctgccgcttc aatttcaaaa agaaatccaa 240
ttgatgcatc aaaaatggcc aactgtcgat caatacattg tttacttcca aaatttcacc 300
aatacacatg cgcccgttga cgttattcgt catcggtttg aacaagtagt caatgaaaaa 360
ggtgtcgttg gtttatccat tggtacccgt cctgattgtt tgcctgatga ggtagtgaac 420
tatttagccg aattaaatga acgcttttat ttatgggtag agttgggttt gcaaaccacg 480
tttgaggaaa ctagcgctgc cattaatcgt gctcatgatt atcaaacata tctagatggt 540
gtggctaaat tacggaaaca cgggattcgg gtctgtacac atttaattaa cgggctacct 600
ggggaaacac ctgccatgat gcgcgaaaat gtgcggcgaa ctattcagga ttcagatatc 660
caagggatta aactgcactt acttcattta atgacaaata ccaaaatgat gcgggattat 720
aatgaaggac gcttacaatt gatgagcaaa gaagcctatg tttcagtgat ttgtgatcag 780
ttagaaatga ttccacccga aattgttatt catcgcttga caggcgatgc tccctttgaa 840
acaattatcg gaccgatgtg gagcttgaaa aaatgggaag ttctcaatgc gattgatgcg 900
gaaatgaaac gccgcaacag ttatcaagga aaatatactg tcatttcagg aaaggaagtt 960
ttcaat 966
Seq ID 19
atgaagaaag aatcaatgtc acgtatcgaa agaaggaaag cacaacaaag aaagaaaacg 60
ccagtacaat ggaagaagag cactacttta ttcagctcgg cgttaattgt ttcatctgta 120
ggaacgcccg ttgcgttact accagtgact gctgaggcaa cagaagagca gccaacaaat 180
gcggaagttg cccaagcacc tactacggaa actggcttag tagagacacc aacaacagaa 240
actacgccag gaactacgga acaaccgaca acggattcgt caacaacgac tgaatcgaca 300
actgaatcat caaaagaaac accaacaaca ccaagtaccg agcaaccaac agctgattca 360
actacacctg tggaatcagg aacgactgat tcttcagtag cagaaattac gccagtagct 420
ccttcagcaa ccgagtctga agcagcgcct gcggttacac ccgatgatga agtaaaagta 480
ccagaagcta gagtagcttc tgcgcaaact ttttcagcgt tatcaccgac gcaaagtcct 540
tcagaattta ttgccgagtt agctcgttgt gcacaaccta ttgcgcaagc caatgattta 600
tatgcatcag tgatgatggc tcaagcaatc gttgaaagtg gttggggagc aagtacgcta 660
tctaaggcac caaactataa cttatttggg attaaaggca gctacaatgg acaatctgtc 720
tatatggata catgggaata tttaaacggc aaatggttag tgaaaaaaga acctttccgt 780
aaatatcctt cttacatgga atcattccaa gataatgcgc acgtgctaaa aacaacttct 840
ttccaagcgg gcgtttacta ttatgctggg gcttggaaaa gcaatacaag ctcgtaccgc 900
gatgcaactg cttggttaac aggtcgttat gcgacagatc ctagctacaa tgctaaatta 960
aataatgtca ttaccgcata taacttaact caatatgata caccatcttc tggtggaaat 1020
actgggggcg gaacagttaa tccaggaaca ggcggctcga acaatcaatc aggaacgaac 1080
acgtactata ctgtaaaatc aggagatacc ttgaataaaa ttgccgcgca atatggtgtg 1140
agcgttgcta atttacgctc atggaacggc atctctggcg atttaatttt cgttggtcaa 1200
aaacttatcg tgaaaaaagg tgcttcaggt aacactggtg gctcaggcag cggtggttct 1260
aacaataatc aatcaggaac gaacacgtac tatactgtaa aatcagggga taccttgaat 1320
aaaattgccg cccaatatgg cgtgagcgtt gctaatttac gctcatggaa tggcatctct 1380
ggcgatttaa ttttcgttgg tcaaaaactc atcgtgaaaa aaggtgcttc aggtaacact 1440
ggtggctcaa acaacggtgg ctctaacaat aatcaatcag gaacgaatac gtactacaca 1500
attaaatcag gcgatacctt gaacaaaatt gccgcccaat atggcgtgag tgttgctaat 1560
ttacgctcat ggaatggcat ttctggcgat ttaatcttcg ctggtcaaaa aattattgtg 1620
aaaaaaggta cttcaggtaa caccggtggc tcaagcaatg gtggttataa caataatcaa 1680
tcaggaacga atacgtacta cacgattaaa tcgggcgata ccttgaacaa aatttctgca 1740
caattcggtg ttagtgtggc taacttacaa gcctggaata acatcagcgg tagtttgatt 1800
tttgctggtc aaaagattat cgtgaaaaaa ggcgccaact caggttcaac gaatacgaac 1860
aagcctacga ataatggtgg cggtgcgaca acatcctaca cgattaaatc aggtgatacg 1920
ctgaataaaa tttctgcaca gtttggcgtg agtgttgcta atctacgttc atggaacggg 1980
atcaaaggcg atttaatttt tgctggtcaa acaatcatcg tgaaaaaagg cgcttctgca 2040
ggtggcaatg cttcttcaac aaatagtgca tcaggcaaac gccatacagt taaaagcggt 2100
gattcacttt ggggcttatc aatgcaatac ggaatcagca tccaaaaaat caaacaatta 2160
aatggcttaa gcggggatac aatttatatt ggtcaaactt taaaagttgg t 2211
Seq ID 20
atgatgattg ttgtactttt aatgagtagt tgcttatttt tatttgtaag ctatcaattg 60
tatttgcgat tacttgtcga aaaactggat tcagaggacc ggacattaaa gaggaaaata 120
aatttctata agtatcagaa aaatagtcgt ttaatgattt atttattgtt tagtgtttgt 180
ctacttagcg tattattgtt aggaattgtt tatacatatt atcaattaaa tcaacgaaat 240
atccgcatgg aacaacggat cgagcgtttg gcacagggcc aagctactca aggtgacaaa 300
ataaaaaaac aagcgattaa aaaaacagcc ttaaatcagt ttccctggaa gcaagctgtt 360
tcggcagaat cggccgctgt cttgactaat tatgaattac aattagcgcg tgaatggcgt 420
ccttacctcg gagagacgag tatcacaatg attagaagtg aaaaaacaca aaccttgaca 480
ctgtctgttt tttcagttgg cttaagttac catgaatttc aaacaggcca agacaatatt 540
gtagcactta ttgcagcgtt gaattcagtt aaagaaatta ccatgattga ttttaatttt 600
acttatcgag atcaagagca aacccttgtc aaatccattg atacgtatgc tcgtgagacg 660
ttggaaacaa atttggagcc tgtcgtgatt agt 693
Seq ID 21
atgtctaact atactattat cgatttaaac caacaaccac acttaattcc gtcagctgct 60
caatggttta gccaaaagtg gcaaatcccc gctgaagcgt atcaagcaag tatgcaggaa 120
atgctaacta gcgaaaacca cgtaccacat tggtgggtct gcttgaataa acagcaggac 180
atcgttgcag gcgtgggcgt aattgataat gacttccaca atcgtgcaga ccttacgcca 240
aacctttgtg cattgtatgt cgaaccaaca tttcgtcacc aaggcttagc aggaattctt 300
ttaacaacag ttggtgattt tctagctaat tctggattta aaaagcttta tttgctcaca 360
gaccatacta ctttttatga aagatatgac tgggaatttt tgacaatggt aaccaccgaa 420
gagcagtctt gcgctagaat ttatcaaaaa caattacgtc aa 462
Seq ID 22
atgaaaaatt cagaaaatga ttatatccaa tctttattcc aaattcttcc cggcttgcta 60
acagctttcc ttgtcgcctg ccttagcaag tttcttgcca tctggttacc tagcttaggc 120
gccgctacga tagctatttt actaggaatc tttttgggaa atacctttgt tcgcggtgcc 180
aatttaaatc gcggaaccaa agtagccgaa agtaaattac tagaattttc tgttgtttta 240
ttgggaacca ccgtgacctt tcaaacgatt gcccaaattg gcttacaagg tgtcgcattt 300
attctcattc aaatgtcgct gaccattatt tttgcctatt tgatcggtaa aaaactggct 360
ttttctgata atatgtcttt attgatggct ggtggtaatg ccgtttgtgg gtcttctgct 420
attgcttcga ttgctccagc aattcaagca gatgaggaag aaaaaggcca aatcattact 480
ttagtaaact tattaggaac cgttttaatg ttgaccttac ccattttaag tgggattctc 540
tatggtacca atttgttggc tcgtagcgca ctaatcggag ggactttaca atcagttggt 600
caggttgtcg ccagtgccaa tatggtcaat gagaacgcag ttcaacttgc gatgctcttt 660
aaaattatgc ggattgtcct actcgtagca gttgtctatt tatttggacg tttcaagcaa 720
agtaagacgg cagaatcaga ggctgagttg gtagaagtca ccaaaaaaag cagcgcccta 780
ccttggtatg tagttggctt tttcattgcc tgtgtcttta atagtttgat tcatttcccc 840
gtcgtgatca gtgagactgc tcatttcttt agttcttggt ttgaaattac tgccttggca 900
gcaatcgggt tacgactcga ttttaaaaag tttttccaag aaggcaaacg tttcttaatc 960
tatggtttat cagtaggaac tgttcaagtc gtcctagcca ttttgttgtt ggcactgcta 1020
cagttc 1026
Seq ID 23
atggcaaaaa caggaatgta tgtaggcctc gatattggta caacttctgt caaagtagtt 60
gtcgctgaat atattgaagg tcaaatgaac atcatcggcg ttggaaatgc aaaatcagac 120
ggtttgaatc gagggattgt cgtcgatatt gatcaaacag ttcaagcaat ccaacgggcc 180
gttcgacaag ccgaagaaaa agccggcatt caaattaaaa gcgtaaatgt tggattacca 240
gcgaatttat tagaagtaga aagctgtcaa ggaatgatag ctgttagcag tgaatcaaaa 300
gaaattacag atgaggatgt gcgcaacgtc gcatctgcag ctttggttcg ttcaactcct 360
cctgaacgtc aaattgttgc aatcttaccg caagacttta ccgtcgacgg ctttgaaggc 420
atcaaagacc cacgtggaat gcttggcgtt cgcatggaga tgtttggcgt tgtctatact 480
ggaccaaaaa caatcatcca taacatccgt aaatgtgttg aaaaagctgg cttaggaatt 540
aacgaattag tgattacacc gcttgcttta acggaaacaa ttttaacaga tggcgaaaaa 600
gactttggaa caattgtcat tgatatgggt ggcggtcaaa caacgacatc tgttatccac 660
gacaaacaat tgaaatttac tcatgttaac caagaaggcg gcgagttcat cacgaaagat 720
atctcaatcg tattgaatac ttcttttaac aacgccgaag ccttaaaaat taattatggg 780
gatgcttatc cagaaagaac ttcagcaaac gaagagtttc ctgttgatgt tatcggtaag 840
tcagagcctg taagagtgga cgaacgttac ctttcagaaa ttattgaagc gcgtgtggaa 900
caaattttga gaaaatcaaa agaagttctg gatgaaattg atgcgtttga attacctgga 960
ggcgttgtgt taactggtgg cgccgctagt atgccaggta ttgtcgattt ggcgcaagag 1020
atttttgaag ccaacgtaaa actatacgta ccaaatcaca tgggcttacg taacccagtc 1080
tttgctaatg tcattagcat tgtggaatat tcagcgcaac taaacgatat ttatcacatt 1140
gccaaatatg caatccctgg tgaaaaatcg aaaccagcac aatcagtcgc tgtctaacaa 1200
gaagttcgct atgacacata tgctgaacag cctcaagaag aatacgaaga attcaacgaa 1260
cgtgagtctg gtgaaaaagt gacaggcaaa atcaaagact tcttctcgaa cattttcgac 1320
Seq ID 24
atggcaaaaa aaacaattat gttagtttgt tccgcaggaa tgagcacgag tttattagta 60
acaaaaatgc aaaaagcagc agaagatcgt ggcatggaag cagacatctt tgcagtatcg 120
gcttctgaag cagatacaaa cttggaaaat aaagaggtga atgttttact tttaggtcca 180
caagttcgtt tcatgaaagg gcaatttgaa caaaaattac aaccaaaagg gattccttta 240
gatgtaatta acatggcaga ttatggcatg atgaatggcg aaaaagtttt agatcaagca 300
atctcattaa tggga 315
Seq ID 25
atgaaaaaag agatcattgt ttatactatt tccgattcac ttggagaaac atcacaaaaa 60
ttgttagctg cagcaagcgc acaatatcca gatatttctt ttcttaatcg atacaatttt 120
tcttttgtca caacagaaga agaattatta gagattttaa aagatgcctt aaaagataaa 180
gccttagttg tcagtacatt agtcagtaaa caactaatca cagcggcgaa agaatttagt 240
gaacgaacag ggttgttata tttagattta atggcgccat tttttgaatt aattcaagcg 300
aaagccggag tagatcctat tgaagagcct ggacgacgcc accaactaga tcgtgcctat 360
tttgataaaa tctcagcgat tgaatttgct gtaaaatatg atgatggcaa aaatcctcaa 420
gggtttcttg attctgatat attgttgtta ggcgtttcgc ggacctcaaa gacgccagtc 480
agtatgtatt tagcgaatca aggctaccgc gtttctaact taccattaat tccagaagtt 540
ccattgccgc caattttgga agaaatggat ccacaaaaaa tgattggttt agtttgttcg 600
ccagaaacat taggacagat tcgtagcagt cggttggctt ccttaggttt aggtaatgag 660
accagttata ccaatgttga acggattgaa caagaattag cttatgccga agagattttt 720
gcgaagtatg gcatcccagt gattgatgta accgcaaaat ctgtcgaaga aacagccttt 780
ttaattaaag aaaaactaga tgaaagaaat 810
Seq ID 26
atggaatgga ttgaaattaa gcacgcaaca caaaataatt tgaagaatat ctctgtcaat 60
atccctaaaa agcaactaac tgttgttact ggactttcgg gttcaggaaa gtcctcctta 120
gtatttgaca cattagccgc tgaatcacgt cgtgaactaa atgatacatt tagttcgttt 180
gttcaaaatt acttacccaa atatggtcgt ccagaagttg aaaaaatcga gaatcttcct 240
gttgcaattg tcattgacca gaaaaaagta gcaggtaatt ctcgttcgac agtgggaacg 300
tacacagata tttatacgtt tttacgtttg ctgttttcac gagcaggttc tccatttgtt 360
ggttattcag atactttttc gtttaatcat ccggatggaa aatgtccaac atgcgatggc 420
ttaggaaaga ttaccgaaat caatcttcat cagctagttg attatgacaa atcattaaac 480
aaagggccta tcgatttccc cacatttaca gttggcaact ggcggtggaa gcgttatgcc 540
catagtggtc tatttgattt ggataaaaaa atcaaggact attctcctga agagttagca 600
ttatttttat atgctccaca acaaaaacta gctaatccac ccaaagagtg gcctcataca 660
gctttgtatg aaggaatcgt cccgcgtatg caacgtagca tattgcatac agacgaaggc 720
aaacgtcatc aaaaatacct taatcacttt gttaccgtaa aaagatgtcc tgattgttta 780
ggaagtagag tcaatgaacg tgttcgtagc tgcaaaatta atcagaaaag tattgctgat 840
gctgttgaca tgccactcac tgaattacat tcttttattc gttcaatgga cctatcctta 900
ataaaaacta ttcaagaaga gctacttgta cgtctagaag cattaattaa tatcggtctc 960
tcctacctca cattaggacg agcaactgaa acactttctg gtggtgaagc acagcggatt 1020
aaaattgcta agtatgtaaa cagcgcctta aatgatatta tgtatatttt agatgaacca 1080
agtgctggct tacatccaaa ggacatcgaa cggatcagtc gtgcattgct caatttaaaa 1140
aataaaggaa acaccgtggt tctcgtggaa cataatccac aattaattag agaagctgat 1200
tttatcatcg atatcggacc tttcgcaggc gaaaatggtg gccatgtcca gttttcagga 1260
acgtatgacg catttttagt ctccaaaacc ttgactagtc aagcgcttca agagccgctc 1320
cctttaaacg accaaccaag aaaagcaagg aagtctttat caatcgaaca tgcgacactg 1380
cataatttaa acaatctatc tgtcgaagtt ccgttaggag ttttgactgt tatttgtggg 1440
gtcgctggtt caggtaaatc atcattagcg gaagaaattt atcaaaaagc ccaggctgat 1500
aaccaagaaa ttattcatct ttcacaaaaa agcattaccg caaatttacg atccacacct 1560
atgacctatc ttaatatttt tgataaggtc cgcaaactgt ttgcggaaga aaatcatgtt 1620
agtccagctt tatttagcta taattccaaa ggcgcctgtc ctacttgcaa ggggaaaggc 1680
ataattgtct ccgatatgtc ttttatggag gatgttacta gtatttgcga aacctgtcac 1740
ggaacgcgtt acaaagaaga ggtgcttcat tatctgtata acggaaaaaa tatcgttgaa 1800
gtactagctt taagcgttaa agatggctat gattttttca aagaccagcc ttttgctctt 1860
tcattaaaaa atttactgga agttggctta agctatctta aactcaatca atcgctctca 1920
acattatctg gtggcgaatt gcaaagggta aaattagcag acacacttca ccaaaaaaaa 1980
gctatctatt taatggatga acctacagat ggtttacact taattgatat ccaacaaagt 2040
cttcaacttt tcaatcgaat ggtagaagaa ggaaacagct tgattttatt agaacatcac 2100
attgatgtga ttaaaagtgc cgactggttg attgaactag gtcccgaagg tggaaaaaat 2160
ggcggacagc ttctttttac aggaacccca gcaaatatgc taaattccac tcattctatt 2220
actaaaggct atctg 2235
Seq ID 27
atgaagaaat taaaaatgtt aggatgcgtc gggttgcttt tagctttaac ggcttgtcag 60
gcgggaacgg gaaactcggc tgatagtaac aaagcagcgg aacaaaaaat tgcaattagt 120
tctgaagcgg ctatttcgac aatggaacca cacacagcgg gggatacgac ctcgacttta 180
gtcatgaatc aagtttatga aggactctat gttttaggta aagaagatga attagagttg 240
ggggtcgctg ccgaagaacc agcgatttct gaagatgaaa ccgtttatac atttaagatt 300
agagaagatg ccaaatggtc gaatgatgat ccagtaacag caaacgactt tgtttatgca 360
tggcaacaag ttgcttcccc taaatcagga tcgattcatc aagctttatt ttttgatgtc 420
attaaaaatg ctaaggaaat tgctttagaa ggcgcagatg tgaatactct tggggttaag 480
gcgctagatg ataaaacgtt agaaataact ttagaacggc ccacccctta tttgaaatca 540
ttactttcgt ttcctgtttt gtttccacaa aatgaaaaat atatcaaaga acaaggggat 600
aaatatgcta ctgatgcaga acatttgatt tataatggtc cttttaaatt gaaagaatgg 660
gataatgcct cttctgatga ctggacctac gaaaaaaatg atacgtattg ggatgctgaa 720
aaagttaaat taacagaagc gaaagtttca gtaattaaga gcccaacgac agcggtgaat 780
ttgtttgact cgaatgaatt ggatgtagtg aataagctaa gtggtgaatt tattcctggt 840
tatgttgata atccagcctt tctttcaatt cctcaattcg tcacatactt tttaaaaatg 900
aacagcgttc gtgatggaaa agaaaatccg gctttagcga acaacaatat tcgtaaagcg 960
ttggcacaag cttttgataa agaaagtttt gtaaaagaag tcttgcaaga tcaatcaacg 1020
gctacagatc aagtaattcc gccgggacaa acgattgcgc cagatggaac agatttcaca 1080
aaactagctg ctaagaaaaa taactactta acctacgata cagcgaaagc aaaagaattc 1140
tgggaaaaag ggaaaaaaga aattgggctg gataaaatca aattagaatt tttaacagat 1200
gatacagaca gcgccaaaaa agctgctgag tttttccaat ttcaattgga agaaaatcta 1260
gatggattag aagtgaatgt tactcaagtt ccttttacta ttcgtgttga tcgtgatcaa 1320
acgagagact atgatttaga attatctggt tggggaaccg attatcgtga tccattaaca 1380
gttatgcgca tctttacttc ggatagtacc ttgggcggcg taacgttcaa gagtgatacg 1440
tatgatcaat taattcaaga aactagaaca acacatgcgg ctgatcaaga ggctcgttta 1500
aatgactttg ctcaagcaca agatattttg gtgaatcagg aaacggtttt agcaccaatc 1560
tacaatcgaa gcatttctgt attagctaat caaaaaatca aggatctgta ttggcattca 1620
tttggaccca cgtacagttt aaaatgggct tatgttaac 1659
Seq ID 28
atgaagcaat taaaaaaagt ttggtacacc gttagtacct tgttactaat tttgccactt 60
ttcacaagtg tattagggac aacaactgca tttgcagaag aaaatgggga gagcgcacag 120
ctcgtgattc acaaaaagaa aatgacggat ttaccagatc cgcttattca aaatagcggg 180
aaagaaatga gcgagtttga taaatatcaa ggactggcag atgtgacgtt tagtatttat 240
aacgtgacga acgaatttta cgagcaacga gcggcaggcg caagcgttga tgcagctaaa 300
caagctgtcc aaagtttaac tcctgggaaa cctgttgctc aaggaaccac cgatgcaaat 360
gggaatgtca ctgttcagtt acctaaaaaa caaaatggta aagatgcagt gtataccatt 420
aaagaagaac caaaagaggg tgtagttgct gctacgaata tggtggtggc gttcccagtt 480
tacgaaatga tcaagcaaac agatggttcc tataaatatg gaacagaaga attagcggtt 540
gttcatattt atcctaaaaa tgtggtagcc aatgatggta gtttgaaaaa agtaggaact 600
gctgaaaatg aaggattaaa tggcgcagaa tttgttattt ctaaaagcga aggctcacca 660
ggcacagtaa aatatatcca aggagtcaaa gatggattat atacatggac aacggataaa 720
gaacaagcaa aacgctttat tactgggaaa agttatgaaa ttggcgaaaa tgatttcaca 780
gaagcagaga atggaacggg agaattaaca gttaaaaatc ttgaggttgg ttcgtatatt 840
ttagaagaag taaaagctcc aaataatgca gaattaattg aaaatcaaac aaaaacacca 900
tttacaattg aagcaaacaa tcaaacacct gttgaaaaaa cagtcaaaaa tgatacctct 960
aaagttgata aaacaacacc aagcttagat ggtaaagatg tggcaattgg cgaaaaaatt 1020
aaatatcaaa tttctgtaaa tattccattg gggattgcag acaaagaagg cgacgctaat 1080
aaatacgtca aattcaattt agttgataaa catgatgcag ccttaacttt tgataacgtg 1140
acttctggag agtatgctta tgcgttatat gatggggata cagtgattgc tcctgaaaat 1200
tatcaagtga ctgaacaagc aaatggcttc actgtcgccg ttaatccagc gtatattcct 1260
acgctaacac caggcggcac actaaaattc gtttacttta tgcatttaaa tgaaaaagca 1320
gatcctacga aaggctttaa aaatgaggcg aatgttgata acggtcatac cgacgaccaa 1380
acaccaccaa ctgttgaagt tgtgacaggt gggaaacgtt tcattaaagt cgatggcgat 1440
gtgacagcga cacaagcctt ggcgggagct tcctttgtcg tccgtgatca aaacagcgac 1500
acagcaaatt atttgaaaat cgatgaaaca acgaaagcag caacttgggt gaaaacaaaa 1560
gctgaagcaa ctacttttac aacaacggct gatggattag ttgatatcac agggcttaaa 1620
tacggtacct attatttaga agaaactgta gctcctgatg attatgtctt gttaacaaat 1680
cggattgaat ttgtggtcaa tgaacaatca tatggcacaa cagaaaacct agtttcacca 1740
gaaaaagtac caaacaaaca caaaggtacc ttaccttcaa caggtggcaa aggaatctac 1800
gtttacttag gaagtggcgc agtcttgcta cttattgcag gagtctactt tgctagacgt 1860
agaaaagaaa atgct 1875
Seq ID 29
atgaaaacgc caacgtttgg aaaaagagaa gagacattga cctatcaaac aagatatgct 60
gcatacatta ttgtttcaaa gccagaaaat aacacaatgg ttctggtaca agcgccaaat 120
ggagcttatt ttttaccagg aggcgaaatt gaaggcaccg aaacgaaaga agaagcgatc 180
caccgagaag tgctagaaga attaggaatt agtgtagaaa ttggctgtta tttaggggaa 240
gctgatgaat atttttattc gaaccatcgc caaactgcct attataatcc tggatatttt 300
tatgtagcga atacttggcg gcaacttagc gaacccttag aaaggaccaa tactttacac 360
tgggttgcac cagaagaagc agtccgttta ttaaaaagag gaagccaccg ttgggccgtt 420
gaaaaatggc tagcggcagc atca 444
Seq ID 30
atggcacgcg tagaaagttt tgaattagat cacaacacag taaaagcacc atatgttcgc 60
cttgctggca cagaacaaaa tggtgatgcg ttagtcgaaa aatatgactt acgtttctta 120
caaccaaaca aagatgccct accaacaggc gcattacaca cgttggaaca tttattagca 180
gttaacatgc gtgatgaatt aaaaggaatc attgacattt cgccaatggg ttgccgcact 240
ggtttttata tgattatgtg ggatcaacat tcaccacaag aaatccgtga tgcattagtc 300
aacgttttaa acaaagtaat caatacagaa gttgttccag cagtctctgc aaaagagtgc 360
ggaaactaca aagatcattc tttatttgca gcgaaagaat acgcaaaaat cgtcttagac 420
caaggaatta gtttagatcc atttgaacgt attctg 456
Seq ID 31
atgaacattt ttgctgtccg tttaaaagag gctttaacag ccaagaacat caaacctagt 60
gatttagcga aaaaaactgg catcggtaaa tcttcgatca gcgattggct agctggtcgt 120
tacgaagcaa aacaagacaa agtttatcgc attgcagatg cattagacat taatgaggcc 180
tggttgatgg gacaagaagt tcccatggaa aaaaatgcct caactatcga ccgcatttat 240
aaaaaattag aaccccaacg acaagccatc gtttatcaat ttgccgaaca acaattacac 300
gaacaacaaa cgcaagcaga aattctctca ttccctcgcc gtgacgaaat gacactggct 360
gcccacgctg gggatccaga aaagatattt tcaaaagaag agatcgagaa aattcacgat 420
tacttggatg aaattgatgc caaatatcaa caatcgattt cttctgacaa aaaagaggat 480
Seq ID 32
atgtataaat ttgttgatac caatcaagca actcattcaa cgcctcttcc ttcagaagcg 60
ttgaatttta acggccaatt tttagaaaaa gtcatccctg gctatcaaac attatcagtt 120
tcaggacgag aattagttcc aagcgaaatt gaaagctatc aattagggat tcgtgatggt 180
aaacgtcacg tttatgcgcg aattccagaa cgagaattaa cagtcaaata tcgcctttca 240
gctgtgaata atgaagaatt tcgagatgca tttaatcatt taaacgttgc tttgtttacg 300
gaaaaagacg tttctatttg gtttaacgat gaaccggaaa tgctgtggtt tggcagtaag 360
tcttcagtga gtgatgtacc cgaaggtgtt aaccaagtaa caggcacctt tactttattg 420
ctttctgatc cgtataaata cacacggagt gatgcgacta gtgtgatgtg gggttcgcca 480
accattacat ttcaagcgaa ttacttaatg gggaatacag gctcaggtgc atttgatttt 540
ccaattttaa ttgaaggcgg ggcttattgg ggatcaacca tgattacctt tcaaaatcgg 600
gcttacacga tgggggattt aggcaaagaa gttcggccaa ttgaaattta tcctacggtt 660
gaaggattaa aagtcaaacc gaccattatt ttaacaggaa ccggacgtgg tgtttggatt 720
aaaacacgga acgatacaat taacttagga gactttgatc gttcggaaat tattatcgat 780
actgaaaatt tttatctgac aaaaaatggt gcaccgatga ttcgaccaat gaacgatttt 840
tatctatatc ccaatgaacc gctgtatatt caagccaaag atagcgactt ccgcttgacg 900
attcgctatc ctaaccgatt tgtg 924
Seq ID 33
atggattttt tcagtagtga agaaatagtt gcaagcttag gtgataaggc agaaatgaaa 60
gggaaattat cgtttgttcg actctgtatt ttaggcatca tggctggttt ttgtattgca 120
ctgggttact tggcgtttat tcgtatcact ggaactgccc ccacagaatg gggaagtttt 180
aacaactttc tgggtggagc tttatttcca gttgggttga tagcgcttac ttttgttggt 240
ggtgagttag caacaggcaa tatgatggta atgacattag gcgtattaca gaaaaaggtt 300
cgagtgggcg ctctttgtta taactggata gttgttttat tgaccaattg tctaggcgga 360
tttcttgtgg cgtatttatt tggccatatt gttgggttaa ctgaaggtgc gtttctggat 420
aaaacagtcg cagttgctca ggcgaaaatt gctgatccac cgctagttgc ttttgtttca 480
gggattggct gtaatatttt tgtttgttta gctgtttatc tcggagcgtt ggcaaaatca 540
tatttaggta aaatgtttgg cttatggttt cctgtcatgg ttttcgttgt ttgtgggttt 600
caacacgtgg tcgctaatgc atttattatt ccagcggcaa ttttttcaca aagtaccaca 660
atcagttggt gggattattt gcagaataca ttatgggtat ttttaggcaa tgcagtaggt 720
ggcagtcttt ttatggccgt accattgatc tttatgacaa aaccagcaac tgtcaaacca 780
agagttgaga aaacgattca gacggaggag ctctatggca at 822
Seq ID 34
atgaatcagc gtattctatc aacattaggg tttgataaag taaaacaaca gttgttgcaa 60
tttattgtga cagctcaggg gacaaacgaa gtttcggaat tattaccaat tgctgatgaa 120
aataaaattc aatcatggct aaatgaaaca caggatggtt taaaagttca acgattacgt 180
ggtggaatcc ccattcctaa attagaaaat atccaaccac acatgaaacg gattgaaatt 240
ggtgcggatt taaatggcat agaattagca caagtaggcc gtgtcttgtc gactacttca 300
gaactgactc gtttttttga tgagttaagt gaaaatgaag ttgattttga acgtttatat 360
atgtggcgag agcaactaga agttttgcct gaattaaatc gccaactcaa acaagccatt 420
gatgatgatg gctacgtcac agatgaagct tcgccagcct taaaggcaat tcggcaaaat 480
attcgtcgta gtgagcagac cattcgagaa gaactagata gtattattcg tgggaaaaac 540
gcacgttatc tgagtgatgc ccttgttact atgcgaaatg aacgctacgt aattccagtc 600
aaacaagaat ataaaaatat ttttggtggc gtggttcacg accaaagtgc ttcaggacaa 660
accttattta ttgaacctaa acaaatttta gaaatgaata atcgtttgcg acaacaacaa 720
attgccgaaa gaaatgagat tactcgcatc ttggcagaac tgtcggctga gttggttcct 780
tatcgccgag aaattacgca taatgcttat gtcattggta aacttgattt tattaatgct 840
aaggtgcgac taggaaaaga actaaaagca gttgttccag aaattagtca agcaaatcat 900
gtagttttta aacaagcccg gcacccacta ttaaatccag aaaaagctgt agccaatgac 960
attgtgattg gagaagaata tcaagcaatt gtgattacag ggcctaatac cggcgggaaa 1020
accattactc tgaaaacgtt aggtctttta caattaatgg gtcaagctgg attacctatt 1080
ccagttgaag aagagagtaa aatggggatt tttacagaag tatttgcgga tatcggggat 1140
gaacaatcga ttgaacaaag tttaagtact ttctcttcac atatgacgaa tatcgtgtct 1200
gtcttaaaga aagtcgatca tcaaagttta gtactatttg atgaattagg tgctgggaca 1260
gatccgcaag aaggggccgc tttagcgatt gccattttag attcattggg cgctaaagga 1320
gcctatgtga tggcaacaac ccattatcct gaattaaaag tgtatggcta taatcgagct 1380
ggaacaatca atgccagtat ggaatttgat gtagatactt tgagtccaac ctatcgttta 1440
ttaattggcg tgcctggccg aagtaatgct tttgaaattt cgaaacgtct tggattagac 1500
aacagtatta ttgaggctgc aaaacaaata atggacggtg aaagtcaaga tttaaacgaa 1560
atgattgaag acttggaaaa ccgtcgcaaa atggccgaaa ctgaatattt agaagctcgc 1620
cactatgtcg atgaatcggc cgctttgcat aaggaactga aagaagccta tcaagtattt 1680
tttgaagaac gagaaaaaga attacaaaaa gcacggaaag aagcaaataa aattattgca 1740
gaagcggaag aaaacgcaga aaccattatt tctgatattc gcaaaatgca attggaaagt 1800
ggccaacaag gtggtgtcaa agaacatcag ttaattgatg cgaaaacaca actttctcaa 1860
ttacaccatg aagaaaccaa gcttgctaaa aacaaagtgc tgaagaaggc gaaagaacag 1920
aaaaaattaa aagctggcga tgqagtaatt gtcaatactt acggccaacg agggacctta 1980
ttaaaagaca acggcaaagg acaatggcaa gtccaattgg gcattttaaa aatgaacgta 2040
tctgaagagg atatgacgcc tgtcgctcca caaaaagaag cgaaaccaag agttacaacg 2100
gttcgttctg cagaaagcag tcatgttggc acacaactag acttacgtgg caaacgctac 2160
gaagaagcat tggcagaagt tgaccaatac attgatgctg cgattctagc gggctatcca 2220
caagtaacca ttgttcacgg caaaggaaca ggtgcattaa gaaccggaat aacggaattt 2280
ttaaaaaatc accgcagtgt gaaaagctat gaatttgcgc cacaaaacca aggtggtaat 2340
ggtgcgactg tcgtaaaatt ccaa 2364
Seq ID 35
atgaaagaaa taactggagc cactcgttta gctgggctat tcgcgaaacc cagccaacac 60
agtatttcac cgttgattca taatacagca tttcaaaatt taggagttga tgctcggtat 120
ctggcgtttg acgttggaca agagacattg ccacaagcaa ttgaagcgat tcgaacgttt 180
cacatgttag gggccaactt atcaatgccc aataaagtgg cggctgtaag ttatatggat 240
gaactaagtc ctaccgctca actggttggc gcaattaata cgattgtcaa caaagatgga 300
aaactttacg gagacagcac ggatggtact ggttttatgt ggagtttgaa agagaaaaag 360
gttgacgttt ttcagaataa aatgaccatc ttaggaacag gtggtgcagc cttatcaatc 420
attgcccaag ctgctttaga tggcgtgaaa gaaatcgccg tttacaacag gaaaagcgcg 480
ggctttaacg acagtcaaaa aaaactggca aatttcactg aacgaaccaa ctgtgtaatt 540
catttaaacg atttagcgga tactgaaaaa ctagcaaaag atgttgctga aagcgtcttg 600
ttagttaatg caacgagtgt gggtatgcat ccacatgcgc atagtagtcc tatagaaaat 660
tatgcaatga ttcaaccgaa gttatttgtg tatgatgcta tttataatcc cagagaaaca 720
cagttattaa aagaagcccg tttacgtggt gcagaaacaa gcaacggctt ggacatgcta 780
ctttatcaag gcgctgctgc ttttgaacaa tggacaggac aaaaaatgcc tgtatcagtc 840
gtaaaacgta aaattgaaaa taga 864
Seq ID 36
atgactaaag gttatgtaaa atcagtaaca gagttaattg gtcaaagccc agttgtgaaa 60
ttaaaacgga tggtaccaga aggagctgca gatgtttttg ttaaactaga gttctttaat 120
cctggtggca gtgtgaaaga tcgaattgct ttaagcatga tccaacaagc cgaagcagat 180
ggtcgcctaa aacctggaca aacgattatt gagccgacta gtggtaatac aggcattggg 240
ttagcaatgg taggtgctgc gttgggatat ccagttaaga ttgtgatgcc agatactatg 300
agtattgaac gccgcaaatt aatgcaagca tatggggctg atctattatt aacccctggt 360
gccgaaggaa tgaaaggggc aattgcaaaa gctacagcat tagcagaaga acacgggtac 420
ttcatgcctt tgcaatttaa taatccagct aatccaatgg tacatgaaca aaaaacagga 480
aaagaaattg ttgatgtctt tggtaaacgt ggcttagatg cgtttgtttc tggtgttggc 540
actggaggaa ccgttacagg agttggccat gaattgaaac ggatttttcc agatattgaa 600
attgttgcag tagaaccaac agagtcgcct gttttagaag gtggcgaacc aggtccacat 660
aaaatccaag gaataggcgc tggttttgtc ccagaagttt tagacaccac cgtttatcaa 720
aaagttgccg ctgtttctag tgaagacgca ttagaaactg ctcgtttaat ggggccaaaa 780
gaaggtatcc ttgtggggat ttcagcaggg gcagcaatta aagctgccat tgatttggca 840
gtcgaattag gcgcaggcaa acgtgtctta gcgctggttc cggataacgg tgaacgttat 900
ctttcgacag ctctttacga atttccagaa 930
Seq ID 37
gtgaaaaatc aacatttaac tacgtcacaa ggaagtccag ttggcgataa tcaaaattcg 60
ctaactgcag gagaatttgg tcctgtccta atccaagacg ttcatttatt agaaaaatta 120
gctcatttta atcgtgagcg cgtaccagaa cgggttgttc atgccaaagg tgctggtgct 180
catgggattt tcaaggtgag ccaatcaatg gcacaatata ctaaagccga ttttttatct 240
gaagttggca aagaaacacc tttattcgct cgtttttcaa ctgtagctgg agagttaggt 300
tcatcagata cattgcgtga tcctcgcggt tttgcgctta aattctatac agacgaagga 360
aattacgact tagttggcaa taatacgccc atttttttca ttcgtgatgc aattaagttt 420
ccagatttta ttcatagcca aaaaagaaat ccgcggacgc atttgaaaag tccagaagct 480
gtttgggatt tttggtctca ttcaccggaa agtcttcatc aagtaacaat tttaatgagt 540
gatcgtggca ttccgttgtc gttccgtcat atgcatggtt ttggtagcca tacattcaaa 600
tgggtcaatg ctgctggaga agtatttttt gttaaatatc acttcaagac gaatcaagga 660
atcaaaaatc tagagagtca attagcagaa gaaattgctg ggaaaaaccc agatttccat 720
attgaagatt tgcataatgc aattgaaaat caagaatttc cttcttggac attatctgtg 780
caaattatcc cgtatgcaga tgcgttaaca atgaaagaaa cactttttga tgtaacaaaa 840
acggtttctc aaaaagagta tccgctgatt gaagttggca cgatgacttt aaatagaaat 900
ccagagaatt attttgcaga ggttgaacaa gtaacctttt caccagggaa tttcgttcct 960
ggtattgaag cttctccgga taaattattg caaggacgtt tgttcgctta tggggacgca 1020
catcgtcatc gcgtgggcgc taatagtcac caattgccaa tcaaccaagc aaaagcgcct 1080
gtaaataact accaaaaaga cggcaatatg cgttttaaca atggcaatag cgaaattaat 1140
tatgaaccaa atagttatac tgaaacacca aaagaagatc ctacagcgaa aattagttcc 1200
tttgaagttg aaggaaatgt tggtaattat agctataatc aagatcactt tacacaagca 1260
aacgctttgt ataatttgct gccaagcgaa gaaaaagaaa acttaattaa caatatagcg 1320
gcatctttag gtcaagtgaa aaatcaagaa atcatcgcgc gtcaaattga tttgttcact 1380
agagtaaatc cagaatatgg agcacgtgtc gcacaagcca tcaagcaaca agca 1434
Seq ID 38
atggcagaaa attatcagca ggcagccaaa gacattattc aattaatcgg tatggataac 60
attatatcgg tcacccattg tcaaacgcgg ttacgtttta ttttgaagga ccatgaacaa 120
gtagatggta aacaactaga aaaaatcgat ttagtcaagg gggtttttta taatggtggt 180
caataccaag tgattttagg aactggtatt gtgactaaag tgtatgatga aatagaaaaa 240
cttggaatca atgttgtgtc aaaagcagag caaacagcaa ttttaaaaaa taatgaaaca 300
ggcatgcgca aaacaatgcg cattttaagt gaaatattca ttccaattgt tccagtaatt 360
gcagcaacag gattgttcct agggttaaaa ggtgttattt ttaacgatac ttttttacaa 420
ttattcggag caagtgtagc caatattcct gaaagctttc aacaaattgt ctctgtcatt 480
acagataccg tctttgcctt tttgccagct ttgattgttt ggtctacctt taaagcattt 540
aatgcgacgc ctgtaatcgg gattgttatt ggtttaatga tggtatcacc cattttacca 600
aacgcttatg cagttgcaac accagattct ggcgtgaaag ctattatggc ttttggtttt 660
atcccagttg tcggtgcaca agggtccgtt ttaagtgcaa ttgctgctgg tataattggc 720
gcaaagattg aacttttttt ccgaaaaaag atgccgaata ttcttgatca aatttttaca 780
ccctttatga ctatgttaat tactttttta atcatgattc tcggaattgg cccaattttg 840
cataccgttg agttaggcat ggtagatgtt gtccaatggt tgattgggtt gcccttagga 900
ttaggtggtt tcgtgattgg tgcctgatat cctttaatgg ttttgattgg tattcatcat 960
acactaacta tggtagaaac atcattatta gcaaatacag gatttaatgc attgattaca 1020
atatgtgcca tgtatgggtt tgccaacgtt ggcagttgtt tagcctttgc gaaaaaagcg 1080
caagatagca aagtgaagtc gacagccatt ggttctatgt tgtctcaatt atttggagtt 1140
agtgagcctg tgttatttgg attacttatt cgttggaact taaaaccgct gctatgtgtc 1200
ttgtttacat caggtttagg cggagcaatt ttagctattt ttcatattca atcaaattct 1260
tatggcttag ccgttattcc ttctttctta atgtatatct atagtgcaca tcaattagtg 1320
atctatttat tagtcgcact tttatctgtc ggtgtatgtt atgcattaac cagtctattt 1380
gcaattccgc aagaagtttt gatttcagat aaagtaatcg aagaggaaga acgcgaagtc 1440
tttgaaatgc aacacaatac cttagatgaa caactttttt ctccggtaac tggttacgcc 1500
atgaatctta ctgctgttaa tgacccagta ttttcaagcg agatgatggg caaaggttta 1560
gcaatcatgc caacagcgaa caaggtttac gcgcctgcag atggtttatt aaacttggtt 1620
gcagaaactg ggcacgctta tggtatccaa acggacgcag gagctgaagt attaattcat 1680
ataggaattg atactgtcac attgggacag gaggtctttc agacacaagt aacccaaggt 1740
catcgtgtta aaaaaggaga tcttttagga acgttcgatc gaaaagccat taaagaagct 1800
gggcttgact caactgtcat ggttatcatt acaaatactt caagttattt aagcgtagag 1860
ccaatgatgt cagatcataa tgaaattacg ccggaacaaa tcattttaaa tctaaataca 1920
cctaac 1926
Seq ID 39
gtggaattag tagataaaga cttacgttcc atccaagaaa caagaaattt aatcagaaaa 60
gccaaagagg cacagcaagt attagcaaca ttttcgcaaa aacaaatcga tgcgattgtt 120
caagcggttt cagaggctac atttaaccaa cgagaaaagt tagcgaaaat ggccaatgaa 180
gaaacaggat ttggtatcta tgaagataaa atcatcaaaa atgctttcgc ttcaaaagta 240
gtatatgacg aaatgaagga caaagctaca gttggtgtga tccatgatga tgctgccaaa 300
aaagtcactg aaattgctgt cccagtgggt gtaattgcag gattaattcc ttctaccaac 360
ccgacatcaa cagtcattta caaagcatta atttcattaa aagctgccaa cagcattgtt 420
ttttcaccac atcccaatgc gttgaaatcg attattgaaa cagtggaaat tatccaaaaa 480
gcggctattg ctgcaggggc accagaaggt tgtgtctcgg tcatcaaaac gccaaccatg 540
caagcgacca gcgaattaat gaaaaataaa gaaacaaatt taattttagc tactggtgga 600
aacgcaatgg ttaaagccgc gtactcttct ggaacaccag caatcggtgt tgggcctggt 660
aatggaccag cctacatcga acgaagcgca aatgttcctc atgcagtgaa acaaataatg 720
gattcaaaaa ctttcgataa cggtacaatt tgtgcatcgg aacaatcaat tattgttgaa 780
actgtcaacc gtgaagcggt caaagaagag ttaattaagc agggagcgta tttcttaagt 840
ccagccgaag ccgacaaatt agctaaattt attttacgac caaacggtac aatgaacccg 900
caaatcgtgg gacgttccgt tcaacatatt gcttcattag ttggtttatc tattccaaaa 960
gatcgccgct taattgttgc cgaagaaaca catgtaggct tgaaatatcc attttcaaga 1020
gaaaaattgg caccgattat tgctttctat acagttgaaa actgggaagc agcttgcgcg 1080
ttatcaattg aaattttgaa aggtgaaggt gcgggtcata caatgggcat ccacacagaa 1140
aacaaagaag tcattcgtga atttggctta agaaaacccg tctctcgttt gcttgttaac 1200
acctctggta cacttggcgg cattggtgct tcaactaact tagtgccagc attaacactt 1260
ggctgtggcg cagttggtgg cagctcaact tctgataata ttggggttga aaatctgttc 1330
aatttacgtc gtgttgcata tggcgtgcgt gatttagaag agattcgtca agaatttggt 1380
caaacatcaa ccacatctgt ggctacttct tgcgaaacaa ccaatcaaga agaactagta 1440
aatgccgtgg tagctcaagt tttagctcgc ttaaac 1476
Seq ID 40
atgaatgaat taaacaaaac accaaaagaa atagccaaag aactagatca atatattgtt 60
ggacagcaag ctgccaaaaa atcagtggcg gtagctttac gtaaccgcta tcgtcgcttg 120
caattagaag aaaatatgca acaagatatt acgcctaaaa acttactaat gattggacca 180
acaggtgttg gtaaaactga gattgctcgt cgtctcgcaa aaattgtcqa tgcgcctttt 240
gtaaaagttg aagcaaccaa atttacagaa gtaggctatg ttggtcggga tgtcgaatca 300
atggtgcggg atttagtaga aaatgcgatc caaattgttg aaaaacaaca atacagtcgc 360
gtgtatgcgc aagcattaaa aaaggccaat caacgtttag tcaaagtatt agtgcctgga 420
attaaaaaag aacaaaaaca ggctggcggt aatcaatttg aacaaatgat gaacatgttt 480
aacatggctc agcaacagca agaagcacaa gaagaagtaa cggaagatat tcgaacgaat 540
cgccgaacaa ttttggaaca gctggaaaaa ggtctattag ataatcgtga agtaacaatt 600
gaaattgaag aaccgaagaa aacgatgcca gctatgaaca atggcctaga acaaatgggt 660
atcgacttaa atgaaacgtt aggcgctctg tcaccaaaga aaaaaatcga acgtactgta 720
acggtgaaag aagcacaaga attattagtg aaagaagaat cagcaaaaat tgtcaatgac 780
gctgatattc atagtgaagc tattcgttta gctgaatcaa gcggaattat ttttatcgat 840
gagattgata aaatcacctc taaaagtcaa caaaattcgg gcgaagtctc tcgtgaagga 900
gtacaaagag atattttgcc gattgttgaa ggctcccaag ttaacacgaa gtatggtcct 960
ttacaaacgg atcacatttt atttatcgct tcaggtgctt tccacttgtc aaaaccaagt 1020
gacttgattc cagaattaca aggccgcttc ccaattcgag ttgaattaga tgatttaacg 1080
gcggatgact tcgtaagtat cttaactgag ccaaacaatg ctttaattaa acaatatgta 1140
gcattaattg gcacagaaaa tgtttcagtc atctttacaa aagaagcaat tgaacggtta 1200
gcacacatcg ctcatgatgt aaaccgtgat acagataata ttggggcgcg tcgtttacac 1260
acaattttag agcgtttatt agaagattta ttatatgaag caccagatat gcaaatgggt 1320
gaaattacga ttaccgaagc atatgtcaat gaaaaattga atgacattgt tcaaaatgaa 1380
gatttaagtc gttacatctt a 1401
Seq ID 41
atggcaaaac gaattatcat tatgaacttt gatattgaaa gtaaatcgta ccaagcattt 60
tccgaaataa aaaagatgca ggcagaaaga cagcttaaag gtgagcaaat ggcggttgtt 120
acgcatgtta atgatggcca gcatcaattt aaaatcaatg attttatcga ttttacaggc 180
aacaatcata catcaaaaga tagcatgatt gggatgctag taggcatatt aggtggtcct 240
ctgggcattc tttttggctg gtttgctgga agtatgtatg gtgcaagcaa agacgccaaa 300
gaaattcaag aagcacaaac ggtttttgaa catgtgattc aaaagattga tgaaggacaa 360
acgggattgt tattaattgc agaagaagaa gacaaccgtc cgctcaacca attggttatg 420
tttgacttag gtggcgaaat cacgcggctt gatttagagg aagtccaaca agaaattaac 480
gatgcgaacg aagttgcaaa tgaagcgaaa caatcgtggc aagcaaaaaa agaacaacac 540
aaagaagcaa catcgaaaga agaa 564
Seq ID 42
atggaaaaac aaacaattac aatttatgat gttgctagag aggcaaatgt atctatggct 60
actgtttctc gtgttgtcaa tggtaatccc aatgtaaaac cagcaacgcg taaaaaagtc 120
ttagaagtga ttgatcgctt agattaccgt cctaacgcag tcgcacgtgg tttagcaagt 180
aaaaaaacga caacagtggg tgtcattatt ccagatgtta gtaatgcatt ttttgcttca 240
ttagcacgtg gcattgacga cgtagctaca atgtataaat acaatatcat tttagctaat 300
tcagatggag acgatcaaaa agaagttact gtattaaaca atttacttgc caagcaagta 360
gatgggatta tctttatggg tcatcgcatt acagatgata ttcgtggtga attttcacgt 420
tcaaaaacac ctgttgtctt agcaggttca attgatccag acgaacaagt tggtagcgtt 480
aatattgatt acacagaagc aacgaaagat gcaacagcga ctttagcgaa aaatggaaac 540
aaaaaaattg cttttgttag tggtgcatta atcgatccaa ttaatggtca aaaccgaatg 600
aaaggctaca aagaagcttt agccgaaaat ggcttatctt ataatgaagg cttagttttt 660
gaatctgaat ataaatttaa agcaggcatt aatttggctg agcgtgtccg taatagtggg 720
gcaactgctg cctttgttac agatgatgag ctagcaattg gcttattaga tgggatgcta 780
gatgctggtg tgaaagttcc agaagaattt gaaatcatta caagcaataa ctcattgctt 840
acagaagtct ctcgtccacg tctatcaagt attacacaac ctttatatga tatcggtgcc 900
gtgtcaatgc gtttattaac aaaactaatg aacaaagaag aaattgaaga aaaaacagtt 960
gttttacctt atggaattga tcaaaaaggt tcaacaaaa 999
Seq ID 43
ttgaagaaat acaatgttga ttggaattac tgggttgtcc gctttttatt tgtaatggcg 60
ctgattgttg gctatttatt aatcacgaat tatcagcact ttgtccatag tgtttcgggt 120
ttattaggca tcttatcccc attcattact gggtttgtga ttgcttattt attgagtgga 180
agtcagaaaa aaatcgaggg attactagaa agagttcctt taccagtcgt taaaaaagcg 240
aaacacggct tgagcgtact ccttttgtac ctgattattt tgtttatttt tgttttgaca 300
ttaaactata tcgttccgct actcattagt aatttagtag atttagcaaa ctctttaccc 360
actttttatg atcacatggt tcagtttgta atgagtttag aagataaagg gattttaaaa 420
acagccgcga ttgaaaaata tttaaatagt gtccttaaag atttgtcgcc agaacgtttt 480
ttaaatcaat ggacacaagc gttgttttca ttaggaacgt tgactaaaaa tgtttcatca 540
ttctttttaa atgcattttt gacgttgatt atctcaatat atgcgctggt atttaagcaa 600
tctattttga catttgttga aaaggctgcc cacaaattgc tgtctgaaaa agtgtacaag 660
caaacacaaa cttggttaaa tacaacgaat aaaattttct ataaatttat cagctgccaa 720
tttcttgatg cttgtataat tggcgtttca tcaacgattt tgttaagtat tttgaatgtg 780
aaatttgctg tgactttggg gattttatta ggtatttgta atatgattcc ttattttggt 840
tcgatttttg cgtcaattgt tgctggtgtg attacgcttt tcacaggtgg tgttactcaa 900
gcaattacgg tgttactagt tttattaatt ttacaacaaa ttgacggcaa tattattggt 960
cctcgaatta tgggagatgc cctaaatgtc aatccgattt taattattgt ttcaatcacg 1020
attgggggag cgtacttcgg cgtcttaggg atgtttttag cagttcctgt tgctgcaatc 1080
attaaaatta ttgtctcgga gtggctaaat gaatcaaaag aaaatgataa gattgtagat 1140
tctattgaat cc 1152
Seq ID 44
atgaaaaagt tctccatacg aaaaattagt gctggttttt tgtttctgat tttagtaact 60
ttgatcgccg gttttagctt gtctgcaaat gcagaagagt atatcgttcc tgccgaaagt 120
cattcacgac aaaaaagatc gttactggac cctgaggaca gaagacaaga agtggcagat 180
acaaccgaag cgccttttgc gtcaatcgga agaatcattt cccctgccag taaaccaggc 240
tatatttctt taggaacagg ctttgttgtt ggaaccaata caattgtcac caataatcat 300
gtggctgaaa gttttaagaa tgccaaagta ttaaatccga atgccaaaga tgatgcttgg 360
ttttatccag gtcgagatgg cagtgcgaca ccatttggca aattcaaagt gattgatgta 420
gctttttccc cgaatgcgga tattgcggta gtgactgtcg gcaaacaaaa cgatcgtcca 480
gatggcccag agttgggaga aattttaacg ccatttgttt tgaaaaagtt tgaatcttca 540
gatacccatg tcacaatatc aggctatcca ggtgagaaaa accacacaca atggtctcat 600
gaaaatgatt tgtttacatc taactttaca gacttagaaa atccattact attttatgat 660
atcgatacaa ccggcggtca atctggttca ccaatctata atgatcaggt tgaagtagtt 720
ggtgttcatt ccaatggcgg cattaagcaa acaggaaatc atggtcaaag actaaatgaa 780
gtgaattata actttattgt taatcgagtg aatgaagaag aaaataaacg tttatccgct 840
gtgccagcag cg 852
Seq ID 45
atgaaaagaa agaaaatcaa gaatcaacta cttgttttaa caagtattct cgttcttctg 60
agtttggaag tcgctccagt tgttacgttg gctgcagaat taccaagtag ctcgctacaa 120
acagctctgt cctcggaaac gacaattact tcggaagaaa aagtaaccga aacaaccgaa 180
acaactgttg ctacatcgac aacgtccact agtagttcgt catctgaatc aagtagttct 240
accgatacga ctaccgaatc aacgagtcag tcaacgacag aaacgacaac cacaaatact 300
agttctgaaa caaaaaaaga acctacagag ccaacggtat catcagaaat cacgcagcct 360
gttgaacaat cgcaaccacc acaagttcct gtgaccaagc aagaacccga agaacctatt 420
caagttcctg aagccaacaa taactttgtt gaagaaaatc aggctgtatc gttaaatccg 480
tccctcaaag tagatgaaat agcttcttca aatttaaaag gctacgaact tccactgttg 540
tcgtcttttg gcgaaaagaa acgggcagtc gtggtcgctg aagcattaag acacgttggg 600
aaaacaaaaa aagaatttaa tttgacggag caagcattga ccagtagttt ccttgcccaa 660
aaaatttacc aacaattatt taaaatagac attggcagta cgccgcaaga acaaatgaca 720
tttggtaaag ttcgttctat agaggaggca gaacctggtg atctgatttt ttggcaaaca 780
gctgaaggaa aaacgcttca aaatggtgtt tatcttggcc aaggaaaata tttaattgcc 840
gcagcggaat cggattctaa agagaaacca gaagtaatcg ctcagttaga gaatatctat 900
actgccaaac aacaaccaga ctcaaaagag gaaaaacgct tggtagtgac taatcctttt 960
aaggagttca tattaacgga gtatggcaaa gaagttttag caacatatgg tgcgtcattt 1020
gaaatgcaga agtcagaaca aaccacagcg tttattaaaa aaattggtga gacagccaga 1080
gaacttggcg aaaaatacga tgtttttgca tcagtaatga tagctcaggc tattttagaa 1140
agtggctctg gcgaaagtca actagccaaa gaaccttatt ataatctctt tggcgtgaaa 1200
ggttcgttcc aaggaaatag tgttagcttt tcaacaaaag aggctgatca gaggggccaa 1260
ctttatacaa tttccgctgg gtttcgcgat tacggtggct ataatgactc actgcaagat 1320
tatgttcaat tactccgaca agggattgac ggaaatcaag atttttataa accagcgtgg 1380
cgttcggaag ccaaaaacta ccttcaagca acgcgttttt taacaggtaa atatgcaaca 1440
gacaagcaat acgataataa attaaattcg ttaattgcgg tttataactt aacccaattt 1500
gatttaccaa aaacagtgga cggtttgatt atccaatcta aaaataagct gtcggaagcg 1560
gaacagcaac aaatgcattt tccagtctat gatgggatca attataatcg aagtggtagc 1620
tatcccgtgg gccaatgtac gtggtatgtc tataatcgtt tcaaacagtt gggcacttct 1680
gtggatgaat ttatgggaaa tggcagtgac tggggaagaa aaggacgtgc attaggctat 1740
caagttagtt cattgcctaa agctggtcgt gcgattagtt tccaaccagg agttgctggc 1800
gcagataatc aatatggaca tgttgctttt gttgaagcgg tcacttctga tggaatcatt 1860
atttctgaaa gcaatgtcat caatgatcaa acgatttcgt atcgtgtctt acctaatgta 1920
attgcttata gtagtggcgt gacctacatt ggcgct 1956
Seq ID 46
gtggatgcag aagcttattt aggtcctgat gatgaggcgg cccatagctt tggcttgaga 60
aaaacgccac gcttacaagc catgtatgac aaaccaggta cgatttattt atatactatg 120
cacacgcatt taattttaaa tatggtgaca caagaacaag gcaaaccaca aggggtgatg 180
atccgcgcga ttgaacctgt tgaaggtgtc gacaaaatga ttgaaaatcg ccaaggacgt 240
caaggcgtgg aattgaccaa tggaccaggt aaattagttg cagccttagg aattgataag 300
caattatatg ggcagtcgat tttttctagt tcgttacggc tcgtgccaga aaaacgaaaa 360
tttccgaaaa aaattgaggc actaccacga attgggattc ccaataaagg tcgttggaca 420
gagttgcctt taagatacgt cgtggctggc aatccatata tttctaaaca aaaaagaaca 480
gcagtagacc aaatagattt tggctggaag gatgaagaaa atgaaaaaag caacaatgct 540
cacatactta gaggaacaac t 561
Seq ID 47
atggcaaaaa agaaaaatgt aagtgtcatt tctgtagaga agccaacgtg gttcccacta 60
acagacgaaa cgggcgcttt tccagtttac ggagcgccaa ttacaatcgg tactgctgta 120
agtatcaaac cagatgttac aacagaaaca acgcctgact atggcgatag tgtagttcaa 180
gatcagtatg ttgcatttgg tggtgcagaa gttactttag aaacaaacgg ctaccaaaat 240
gaagttttag ctgaaattac agggggaaaa aaattaaagg gtggcgtatt gcggtctgcg 300
gatgatattg catcagatgg cgcatttgct tatcgtcgcc gaaaatcgaa cggtaaatat 360
cgctatacga ttttctataa aggaaaattt gctttaacat ctgatgaaac atctacatta 420
gaaggaagtt cagtatctta tacccatcca gaatggacgg ggtctttcgt tgatgttcca 480
gggttgggtt atatgtattc cgtggatgaa gacgatgaag gtgttgactt agagatgatt 540
aaaaactggt ttactgaggt aatggatcca cgtaaagaaa atactactgc tgttactggt 600
gtaactttag accaaacaga gttaaattta aaagttggcc aaacagcaac cttaacaccg 660
acaattacac cagataacgc ctcaaataaa aaatatcagt tccgttcaga aagtgaggct 720
attggaactg taacaccaat tcaagggaag gttactgctg taggagaagg gacaacggaa 780
atcgtagtca caacagaaga tggtaacttt accgcaaaat gtacattaaa tgtaacaaca 840
gcagat 846
Seq ID 48
atgtttcttg gcctgatttt tattttattc ggtgtctttg gactctttcg cttagggttt 60
ttaggcacgt tattagcgaa ttgtttacgc ttagttgtag gtaatacctt tccatttgca 120
gcgattttac taatcctgta tggcttatta gtcatgattt atgggaaaga ttttccgtta 180
aaaagagggc gccctatttt tggggctgtt ctgatttata ttagtgtttt gttatttttc 240
catgccttta tgtttcgaaa tgttagtggc agtcaaccag atattttagg taacacttgg 300
gaatttttgc aaagcgatct aaaagctaac caagtgacgc aaaacgtagg gggcggtatg 360
attggcgcac tgctttatca aggaacgtac tttttggtgg cacaatttgg tagctattta 420
attgccacct tgctactgtt agcgggtatt ttcttgatga gtatgtggga ttttcagcaa 480
attgttgatc atttccaaag tattcaagat cgcctaagtc atgtttcggc aaaatcacag 540
gcgcgacaag aagaaaaaga ggcgaagcga gcagcgaaga aagaagccaa agctgcagaa 600
cgccaagcaa aaattgaagc cgctgcccaa caaaaattac aagagcggga acgcatggaa 660
caagcggcag ccgagcgctt aacaaaaaca cctgttgaaa cccaccaacc aatggtggaa 720
gaaccggccg caccaacgcc tgttcaaatt gattcgtttc aacagcaaaa tcaggcgatg 780
cctgtaccac caattgcagc aacaaaaccg caacgagagc aggaagaaga ggcggcagat 840
gaagctggtg ttctcgaatt tgaaatttct gaagaagcgg aagatcgtga ttatcaacta 900
ccaccaactg atttgttaga tacaattcaa gcaacggatc aaagtggtga atatgaaaaa 960
atcgaaaaaa atattggcgt cttagaacaa accttcaaga gttttggtgt tgatgctaaa 1020
gtagtcaaag ctagtttagg accatctgtc acaaaatttg aagttcaacc cgctgttggg 1080
gtaaaagtta gcaaaattgt taacttgacg gatgacattg ctttagcgct tgcggccaaa 1140
gacgttcgta tggaggctcc gattcctggg aaatcactaa tcgggattga agttcctaat 1200
agtgcaatta gtacagtatc ctttagagat attgttgagg cacaaccaag tcatccagat 1260
aaactattag aagtaccttt aggtcgagat atttcaggga tggttcaaac tgcagatcta 1320
tcaaaaatgc cgcatttatt aattgcggga tcgacaggga gtgggaaatc ggttgctatc 1380
aatggcatca tcacaggtat tttgatgcaa gcgaaacctc atgaagtaaa attaatgatg 1440
attgacccga aaatggtaga attgaatgtg tataacggga ttcctcattt attaacgcca 1500
gtggttacta atccgagaaa agcggcccaa gccctgcaaa aagtagttca agaaatggaa 1560
tttcgttatg aaaaatttgc agcaacaggc gtccgaaata tcactggtta caatcaacta 1620
attcaacaga aaaatgcgga agatggcgaa aatcgtccaa ttctaccatt tattgttgtc 1680
attgttgatg agttagctga tttaatgatg gtggcaagta acgaagtaga agatgcgatt 1740
atccgtttag cccaaatggc acgggcagca ggtattcata tgattttagc gacacaacgg 1800
ccaagtgtgg atgtcattac aggaatcatc aaagcaaatg tcccttctcg aatggctttt 1860
gcggtctcaa gtgggacaga ctcgcgtacc attattgata ccaacggggc ggagaaacta 1920
ttaggtcgag gcgatatgct cttcttacca atgggagaaa ataaaccaat tcgtatccaa 1980
ggggccttta tctctgatca agaagtcgaa cgagtggttg cgtttgtaac ggatcaacaa 2040
gaagctgaat atcaagaaag catgatgcca acagatgaac cgaccacttc aggcggtggc 2100
gaagctcctc aagatgagtt gtttgaagaa gcgaaaaact tggtggttga aatgcaaaca 2160
gctagtattt cattgctaca aagacgtttt agaattggct ataatcgtgc ggctcgctta 2220
gtagatgaat tggaagcaca cggagttatt ggaccatcag aaggtagcaa accaagaaaa 2280
gtctttctac aagcggaatc agaagaggca gcaaccgaga cgccagaaca a 2331
Seq ID 49
atgagaaaaa gctttaactt agctgttcaa gcgttaaccg ttcaatatca aggacggacc 60
gctttaaata atatccacgt tactattccc tccggtaaaa ttactggaat tatcggacca 120
aatggtgctg gaaagtcaac atttattaaa gggttattag gcttgattaa aacaaaggaa 180
cgtgatgttt tgttaaataa tcaagcgagg gaccaacaaa aaacaaccat tgcctatgta 240
gaacaacgta gcgccttgga tcttagtttc ccaattagcg tttttgaaac ggtcttgcta 300
ggaacctatc caaacttggg actattaaaa cgcccaggaa agaaagaaaa gcaagcagcc 360
atggctgcat taaaaatggt gcaattagaa gactatgcgc aacgccagat tggcgaactt 420
tctggtggcc aattacaacg tgtgtttatc gcccgtgttt tggcccaagg tgctgaggtg 480
atttttttag atgaaccttt cgtcggcatt gatatgtcta gtgaaaaagt gattatggat 540
attcttaaat cattaaaaaa tcaaggtaaa ttgattatca ttgttcacca tgatttgcac 600
aaagtgtccc actattttga tgaattaatc gttttgaaaa accggctaat tgctgcgggt 660
cctgttgaac aaacatttac tgcagaaacg ctccaagaag catacggtga tttgttaggt 720
gatttattaa tacagggggt tgcaaaa 747
Seq ID 50
atgattggat tagaattgac ttttacagtc cctcaagcag atgaagtcat tcggtcatta 60
gttgaaacat atcatgacga gaaagatatc gtgattggcg cgggcacggt gttagatgct 120
gtgacggctc gcctagctat aatggccgga gccgaattta ttgtaagccc tagctttaat 180
cgggagacag ctgaaatttg taacttgtat caagttcctt acttgccagg atgtatgaca 240
attactgaga tgcagacagc tttgaagagc ggagtagata tcgtgaagtt atttccggga 300
agtacctatg gtccgagtat tatctctgca ttccaagcac ctttgccgta cttgaatatt 360
atgcccacag gcggtgttag tctggataat atggcgtcat ggtttgaggc gggtgtgacg 420
gcagtgggtg ttggcggaaa cttattagct cacgctaaaa ctggtgagtt tggtaaagta 480
acggctttgg caaaacaata tatggccaaa tttagagaaa taaaggaaaa c 531
Seq ID 51
ttggcaggcg taaccaaaga gcagattgca aaagcaaagg aatgggatct gctctcttat 60
ttgcaggcct atgagccgca ggaactgaaa aaaagcggcc cccgcgaata ctgcaccaga 120
acccatgaca gcttaaaaat ctccaacggg aaatggtgct ggaacagccg gggcatcggt 180
gggcgcacgg cccttgatta tctgatcaag gtgcgcggca tggactttgt aggcgcggtg 240
gaaaccctgt gcggctactg tgcgccaccg cccgaaaaag cgccgcccat aaaagaaaaa 300
cagccaaagc ccttccgact gccccaggcc agccgatgtg catccgctgt tgtgggctat 360
ttgcaggatc gcggcattga cccggaactg cttggcgtgt gcatggaagc cgggatttta 420
tacgagagct gcaaatacca gaactgcgtc tttgttgggc gcgacacagc agggaacgcc 480
cgcgccgcca gcctgcgggg gacgcgggac ggcttccgca tggatgtgga gggaagcgac 540
aagcggtaca gcttctgtct gccggcaggc aaggcagact gcccccggct ggcggtggcg 600
gaaagcccta ttgacgcgct ctccctcgcc acattagtaa agctgtccgg cggcgaacca 660
cgggacagcc attatttatc cctgggcgga accggccccc gcgccctgat acagtttctt 720
cacgaccacc cccacgttac ccaagtcagt ctgtgcctcg acaatgacaa ggccggcttg 780
gagggcatgg aacggctcac ccaggaaatc cagaacgatg cggaactttc caggcgggtg 840
aagctggttt accccaaccc gccgaagcag ggcaaggact ataacgagtt tctctgcacc 900
cacgttaaag cggtacggac ggcacagcgg cagcgggacg cggcgcgg 948
Seq ID 52
gtgaggataa aggaggggcg cacggtgggg ctgttcacca aagtttcccc ggaagaaaag 60
gcggcgattg accgcaaaat ggaattgctg ggaacctcca acctgcgggc ttacctacgc 120
aagatggcgg tggacggcta catcgtgcag cttgacatgg gcgcggtgct ggaactggta 180
aaattgctgc gttccgtttc cagcaacgtc aaccagatcg cccgccggtg caacagcacc 240
cacaacctct atgagcagga tgtggaggac ttgcggcagg gctactctga aatctggcag 300
ggcgtgaacg acctgctgaa gaaatttgaa gcactg 336
Seq ID 53
atggcgaata agctacaaac cgtgtcgcag cttgcagacc agaccgccaa ggccgtcacg 60
cggaacgcgg agggctggaa aagctatctg accactgcat cgcggcttta taaatactcg 120
tttgacgaac agcttctgat ttacgcccag cggccggacg ccaccgcctg cgccagcatg 180
gaattatgga atgaggatat gcggcgctgg gtcaaggccg gctccaaggg catcgccctg 240
atcaaaaagg acggcatccg cccacggctg acctatgtgt tcgatgtggc ggatacccgc 300
ccggtgcggg gcgcgaaaat gccctatctg tgggaaatga aagacggaca ccatgccgcc 360
gtcatggata cactggcacg gcgttacggg gaaacggaaa agaaggacat cggatctgcg 420
ctgatggagc aggcgaaaca cgccgtagag gaagtgtacc gggaacacct ttctgacctt 480
gcctatgacg cccaggacag tgttttggag gggctggacg atttcaacct ggaagtgcgc 540
ttccgaaatc tcctgaccgc cagcgtccag tatacgctcc tttcccgctg cggcttaaac 600
cccgccgatt acctggagga cgaagatttt gacggcatcc atgaattttc cacccccgcc 660
gtcctccacc acttaggcag cgccgccagc gaagtgtcca tgaatatgct gttggaggtc 720
aagaaagcaa tccggcaggc ggaaaaagaa aaggcacaga accggcagaa aatcccgaaa 780
aatcccgaaa aaccacttgc aaaagaaccg gtgatcggtt atactaaagt caaggaacag 840
tttaacacat taaaacgcga aagcgaagaa aggagcatac aaaatggcag aactggcata 900
caagaggacg ggcgactacc tgattcccga cttggtgatg gacggggagg acgagatgga 960
ggaaatgccg ctgggcaagt acggcaggct gcggcagacc tttcttcagg aacaccacaa 1020
ggggacatac acctcgatgc tgctgaccgg gcggctggaa ccccacctgc gggagatcga 1080
ccggcaggcg caggaacagg cagaccggat cgtggcggaa ttaaagaaac agaacggcgt 1140
ggacgagggg atgaaagccc gcgaccagat gggatgggtg caggcagtca acctgtttct 1200
cgcccaggcg gaggaaaccg tactgaacga gatcgtttac aagtaaatca ggagaaccag 1260
caggaagcgg cgggcgaaca gtccgccgtt tctgcgtctg aaaagccggc atttacacag 1320
ttttctttat tccccactgt ggaacagcag attgaaacta ttgctcaggc acagaaaacc 1380
gagcaggaac tgcgccccgc cgtttcctcc ggcaaggtca cggatgcggt cattggccgc 1440
gccctcacaa gcggcggcaa cgagccggac agcatcctgc gtatcgtcgc ttttttccag 1500
aaagacccta cggagcagtc tgccgccgat tttctgcaaa aggaatacgg cagcggcggc 1560
aaggggctta aaattgccgg gcatgagtat tcgctgtggt tcagccatag cggtatccac 1620
atcgcgccgg ggcgctctgc cgggggcggc tcccttgtca cctggaagca ggcggcggca 1680
cagatccggc agcttttgaa cagcggccag tttgccagcc aagacaaaat cgacgcggcc 1740
cgtgacaatg aatttcggga attatcggaa aaactgtggt ttctgcggca gaatttcagc 1800
gaggaagcaa aggaaaaggg cttgctgccc accattgacg cgctgtacgg tggtttcccg 1860
gacagcacgg ctaaaattgc cgcgctcctg aaagacccgg cagaacgagc caaaattgca 1920
caggaagtcc ggcagtttgc catctcccat caggagaacc cgaatctgct gcgcttccgc 1980
cagcggcccg cgccctccga actgtttacc cgcctgaccg atatggataa gccggtgacg 2040
gcatttcatg gggtggaggg ctttgcgcct gcccgtggag cttttgtcac cgaggacgag 2100
attacccgga tgttcactgg cggctctggc gtttccgagg ggaaattccg tatttacgcc 2160
tactttatgc aggggcatga tgccaaagag tgcatcgaat ttctgaaaaa cgaatacggc 2220
attggcgggc atggctacat tggctatgat gaatggcacg acggcaaagg catcaagctg 2280
tcccgcgccg acgatttttc cggtggcaat tatgatacgg ttacgctgaa ctggaagcag 2340
gtacaaaagc ggattgccgg actgattaaa acggatcggt atctgaaccg cagggaaaag 2400
gcatatctgc cggaatatga aaaaatgcag cttgctcgga gtctatatac cttccaatac 2460
tacgacccca acgatgccag caagacaatc ccccacgact gggatgttga cgcggcaaaa 2520
aaggtattcc gcccgctgtt agatgacccg gaacaatgcg cggcgcacta tgaaaaaatg 2580
gtgaaagccc tggcaatggt atccccggat gagcgcgcct attccctgat ggagccgacg 2640
cttcaaaaaa tgggcgcata cctgcggggc gaatactcct tattcacccc tctgcctgat 2700
gccgtcttgc aggaggaacg gcagaaaaag cagaaacaga aacagaagga aaggcaggat 2760
tccgccagac aaacggctga acctgcaacc ggccttgctg ctgcggcaaa agccctttcc 2820
cgcaagaaac agcctgcccc acgggaggat gcagacgggc agcttacttt agatatgttc 2880
gggctttcgt ccgaaccaga acagccagcc gcaactcctg aaccggagcc ggaactttcg 2940
caggcagaaa ccggcgcatc tgaaacggcc gtccctatcg catcaaagcc ggaaccaccg 3000
caggaaacgc cgaacatacc agaaggggct gcgcccataa tggccgaaag ccccgctgac 3060
cgctatgatt taggctatgg ccatatgggg aacggcctga ccgtctggaa ccgtctggag 3120
gaagaacacg gcgactacaa aacagttgcc catatcgccc ctgaccgtac cgtgactttt 3180
tacgacgcgg atatgccgga ggaaatcagg gaaaaaattc aaaaggttgc cgctgccact 3240
gaaatgtcca tttctgccac ccaggacacg ccagtatttt ccaccccgcc ccaggagccg 3300
gaacgggtgc agcctgggaa ctcggagcca gagccggaaa aggtgcagga cgcgcccgct 3360
gcggtcaatc cagagccgga aaccggggac agcaatattg taccatcccc cgcgcaaaag 3420
gcgggcgcag aacccaccgg cacaggttcg ccgcagaccc aggaagcccc aaaaacagcc 3480
gccgcagacg gtctgaacct tacgcccaac gtggaggaat acctgaacct gaaagcccaa 3540
taccctgaca aactggtagg cgtccgggta gggaaaacca tgctgtttta cggcacagac 3600
gcagaggaag ccgcccctgc gctgggaacg aaaaccatca cccgcgatat tcccgacctg 3660
gggatggtgt ccatcaccgg agccaatggc tggcagtccg tcttaaaaaa gctgctggaa 3720
catggaaaaa gcgtggtgct ggcgcggcct gacacggaac gcggcggcga tgccccctat 3780
gaaatcatca aggaacgcag cgcggcggat tatatccctg tcggcatgga actgaccatt 3840
gacgggcggc tgatgaagat tgacagcgtg gactataacg ccggaaccgt cagcctgcaa 3900
gatatggaac tgcggggctg gtatcccatt ttccgcagtg aaagcatccc ctttgtgcgg 3960
cagtttgtag aggaagtcca gcaggagcat tttacagcgg aacccatgca ggagccggaa 4020
cgccccgaaa cctccgattt agatacggca aaacagctaa ttgaacagtt tgcctacgcc 4080
gaatatggca gcgatgatgt ggatttttcc gatttggagc atatcgggat tgcctatggc 4140
acgaccgagg acggcggttt agaagtccag gtggacgtaa acctgctgga tttctccatc 4200
agccagtccg tagacgggaa atgcgtggaa acacggcagt atggcagcct gcgggaactg 4260
attgatatgg aacttgcttt ccttgactat gatatgctgg tttccgtgga accggatatt 4320
gaagaacggt taaaagccga actgaaccag cggatcaggt ggtcagaaat ggcaggtgcc 4380
agagagggcg tggagccaac agagccggaa atttttccag agaaggacgt ttctccagaa 4440
caggaagcac cgccggaact ggtggagatt gacggcgggc agattacgga aaccccggcg 4500
caaagacaga cccgccgccg cgcccaggag gaagtggaca gccgggtatt ccccagcgag 4560
attattttac agccgctccg tctggagccg gaacgccaca acttccgcat caccgatgaa 4620
aatttaggcg caggcggcga aaagaccaag taccagtaca acgtggaagc catccgcacc 4680
ctgaaacaga ttgaagccga aaaccgcctt gccacaccag aagaacaggc cattttatcc 4740
cgttatgtgg gctggggcgg catttcccac gcctttgagc caaacgaccc aaaatgggca 4800
aaggaatacg ccgaactgaa agaactgctc acccctgggg agtaccagtc tgcacaaagc 4860
acggttctca acgcccacta caccagcccc accgttatcc aggcaatcta caatgcggtg 4920
gaacagatgg actttacgcc ggggacggtt ttggagccgt ctatgggcat cgggaacttt 4980
ttcgggatgc tgcccgaaaa actggcggca gcaaagttat atggtgtgga actggacgac 5040
ctgaccgggc gcatcgccag acagctatac caaaaggcgg atataacggt agacggattt 5100
gagcgcaccg accacccgga cgatttcttt gaccttgcgg tgggcaacgt cccctttggc 5160
agctatcagg tgcatgacaa gcggtacgac cgtcaaaacc tgatgattca cgactatttc 5220
atcaccaaaa cgctggacaa ggtgcgtccc ggcggcattg tggcctttat caccaccaaa 5280
ggcacaatgg acaagaaaaa cagcaaggcg cgggaggcgc tggcacaaaa agccgattta 5340
ttgggtgcag tccgcctgcc cagcaatgcg ttcaaggcca acgccggaac cgaagtcaca 5400
acggacatcc tctttttcca gaagcgcgac cgcatcccgg aaaaactccc ggaatgggtg 5460
gaggtcgggc agacagagga cggcatcccc ctgaaccgct actttttaga ccacccggaa 5520
atggtgctgg ggacaatgac aatgggacgc agtatgtacg gcaatgaaac tgaaaccgcc 5580
tgccagccca tccccggcgc agacctttcc gggcagcttg cagaagctat ccggcatatc 5640
gcaccgcctg accgggaact gttagaagtg gattccgggc aagacggcga ggaactggaa 5700
agcatccccg ccgaccccac tgtacgcaat ttcagttaca ccctatccaa tgataagctg 5760
tacttccggg aaaactcccg catgacccag gccgtccttg gcaaaacccc cacggagcgc 5820
gtcaggggca tgatcggcat ccgggacagc gcccgccgcc tgatcgactt acagcttgcc 5880
ggcgcagacg acacagaaat ccagtccgag caggcaaaat taaaccggct ctatgatgcg 5940
ttcagcgcaa agtacgggct tcttaacagc actggcaaca agctggcctt tgagcaggat 6000
tcctcctatc cgctgctctg ctctcttgaa atcatcaacg aggaggggaa gctggagcgt 6060
aaagcggata tgttcaccag gcgcaccatc cagacccacc gcgccgttac cagcgtggac 6120
acggctgtgg aggcgctggc ggtatctatc ggtgaaaaag cctgcgtgga tttgggctat 6180
atggcatccc tcatgggcgg ccctgaaaaa atcccgcaga ttgtggagga cttaaaaggc 6240
atcatcttca aaaaccccgc caccggcccc tttgacctgg aggacggcgg cgcacactgg 6300
catcagggct ggcagacggc ggacgaatac ctttccggca acgtccgggc caagttagcg 6360
attgcccgcg ccgccgctga ggaaaacccg cagtttgcca tcaatgccga aaagctggaa 6420
caggtgcagc ccaaagacct gacggcatct gaaatcagcg tccgtatcgg cgcgtcttgg 6480
attgacccgg agtattacca gcaattcatg tttgaactgc tgcatacacc tgtgtattta 6540
caggacagaa aaatcaagct gcaatattcc cctaccaccg gcgaatggaa cgtgcaggga 6600
aaaagcacag acaaccggga taacgtgcgc gtctatgcta cttacggcac caagcgcatc 6660
aacgcttatg aaatctttga gcagacctta aaccagcggg atgtacggat atttgataag 6720
acagaagtgg acggaaagga ggtgcgcgtc ctcaacgaaa agcagaccgc cattgcccag 6780
cagaagcagg aagccatgtg cgaaacgttc aaggactgga ttttcaaaga cccggaacgt 6840
cgggaaaccc tttgcaggcg gtacaacgag aaattcaact gcatccgtcc acgcgaatat 6900
gacggttccc atatccgttt tgctgggatg aacccggaaa tctccctgcg tacccaccaa 6960
gagaacgccg tggcgcggat gctctatggg aaaaactcgc tgcttgccca ctgtgtcggc 7020
gcgggcaaga cctttgaaat gattgccgcc gcaatggagg gcaagcggct gggtctgaac 7080
cagaaatccc tattcgtcgt tcccaaccac ctgacggaac aatggggcgg cgattttttg 7140
cgcctgtacc ccggcgcaaa ggtgctggtt gccacgaaac gggactttga gcctgcccgc 7200
cgcaagaaat tctgcgcccg gatcgccacc ggcgactatg acgcgataat tatcggacac 7260
tcacagtttg aaaaaatccc cctctccccg gaacggcaga aagccgtcat taaggggcag 7320
attgatgaaa tcgtcaccgc cattgccgaa gcaaaggcgg aggacggcga gcgctacacc 7380
atcaagcaga tggagaaaac aaagaaaaac ctggaagcca agctgcaaaa gctggcggac 7440
ggaaagaaaa aagacagtgt tgttaccttt gaggaattgg gcgttgaccg tctgtttgtg 7500
gacgaggcgc acggctttaa gaacttgttt ttacacacaa aaatgcggaa tgtggcgggg 7560
attgcccaga cggacgcgca gaaatccagc gatatgtttg ccaagtgccg ctacatggac 7620
gaaatcaccg gcgggcgcgg catcgtgttc gctaccggaa ccccggtgag taactcaatg 7680
gtagaattgt ataccatgat gcgctacctc cagtttgaca ccctggagca gaacggccac 7740
cgccatttcg acgcatgggc ggcagacttt ggcgaaaagg tcacggcgat ggaattaaag 7800
ccggagggca gcggcttccg ctccaagacc cggtttgcga aattctataa cctgccggaa 7860
cttatttcta tttggaaaga agccgccgac atccagaccg ccgatatgct gaatctcccc 7920
gtgccaaagg cggaatatat caccgtcacc acggaaccca gcggattcca gaaagaaatg 7980
gtggaggaac ttggcgagcg tgcggaaagt gtgcgcggcg ggcaggttga cccccatatc 8040
gacaatatgc tgcggattac ttcggacggg cgcaagctgg cgctggatca gcggctgcaa 8100
aatccgctcc tgcccgacga cccggacagc aaggtcaacg cctgcgtgaa aaatatcgtg 8160
caggaatggc aggccagcac ggagatttta ggaacacaat tagtgttttg cgacctcagc 8220
acacccaagg gggacggctc attcaatgtc tatgatgaca tcaaggaaaa gctgattgcc 8280
aaaggcatcc ccgaagcgga aatcgccttt atccacgacg ccaacacgga aacccaaaag 8340
gcagaactgt tcgccaaggt gcggcggggg caggtgcgtg tcttaatcgg ctccacggca 8400
aaaatgggcg caggcacgaa tgtgcagaac cgcattgtcg cgtcccatga cctggattgc 8460
ccctggcgtc ctgccgattt ggaacagcgg gcgggccgat ctctgcgcca gggcaacatg 8520
aatgcgtcgg tgaaaatgtt caagtatgtc accaagggta cattcgacgc ttacaactgg 8580
gggctggtgg aaaacaaaca gaaattcatc ggtcagatca tgacctctaa aagccctgcc 8640
cgctctgccg aggatgtgga cgccactgcc ctttcctatg ctgaggtcaa ggcccttgcc 8700
accggcgacg accgtatccg cgaaaaaatg gatttggatg tgcaggtttc caagctgaaa 8760
atgctcaaag ccaaccacac ctcccaacag tacgaaatgc aggacaaagc gctgaaatac 8820
tacccgcaga aaatcgcgga aaccaagctg ttaatcgaag cgctgacggc agatttgcca 8880
accgtgcagg cccacccggt caaggacgat gcgttttcca tgactgtcat ggggcagacc 8940
tacacggagc gcaaggcggc gggcgaagcg gtgattaaag cgtgtatgct gatggacgac 9000
ccggaaaaaa ccgtggattt gggcgagttt cgcggctttc ccatgcagct aaggtgtgac 9060
ggcagcaaat tccgcgtcac catgaagcag aacctgacct attccgccga actgtctgac 9120
gatgcggtgg gcaacgtcac ccgtattaac aatgcgctgg aaagcctgac ggagcggttg 9180
gacgcacaaa aagcacggct tgccacactg gaaagcgaac tggaaaacgc caaagaagaa 9240
gccgaccggc cattcccaaa agaagaagaa ctgcgggaaa aatccgcccg cttaaaccag 9300
cttaaccggg aactgtccag gccggacaaa aaggaggatg agcaggaggt tgaagaaagc 9360
cctgatttgg aggacgccgg aaaacccgcg ccggttgtgc cgatgcccgc tgtggcggct 9420
ggctataaac cctctatccg tcaggcaatc cgtaactatg aaccgcccac gccggtacat 9480
tccggcatgg agcgcacccc gcgaaaggag gcggtgctg 9519
Seq ID 54
atgcctgtgt tgacttcggg gcagcaagaa aggaaaagca aacagagaaa aatccgcaac 60
tcagagtatt acgacatgga aggtactttt gacaggctgt atgcagaaag taagaaagat 120
aaaaccttca accatctgat ggaaatcatc gagagtgaag aaaatatcaa gcttgcatac 180
agaacgataa agaaaaatac aggaagtgat acttcgggag tggacaaaag gacgatagcc 240
gaccttgcaa agttaagcga ggaagaatac gtccgtctca tacggaaaca attcagtaat 300
tatcatccag gacccgtaag gcgagtggag ataccaaagc ccaatggaaa gaccagacct 360
ctgggaattc caactatcgt ggatcggatc gtacagcaat gtattttgca ggtcatggag 420
ccgatatgtg aagcaaaatt cagtgagaac tccaacgggt tccgcccgaa ccgttccgct 480
gagactgcta ttgcacaatg catgaggctg atacaggtac agcatctgta ccatgtggtt 540
gacctcgata ttaaaggctt ctttgacaat atcagccata caaagctgat tcgtcagata 600
tgggcattgg gaatccggga caaaaagctg ttatgcatta taaaagagat gctgaaagca 660
cctgttgttc tgccaaatgg agagaaaaca tatcccgcac ggggtacccc gcaaggcggt 720
attttgtcgc cattgcttgc aaacatcgta ttgaatgaac tggattggtg gattgcctca 780
caatgggagg agatgccaac aaaaacaaaa ttcaaaacaa ggagcaatgc gcagggaaca 840
gagattaaga gccacgccta cagggctctg cgccggagca gattaaaaga gatgcatgca 900
gttcggtacg cagatgattt taaaattttc tgcgcaacac atgaggatgc tgtcagagca 960
tataaggcaa cagagttatg gctaaaggac aggctggggc tggaaatcag ccctgacaaa 1020
tccaaagtag ttaatctcaa aagacagtat tctgattttc tcgggttcaa gctgaaagtc 1080
cgtaaaaagg gtaagaaata tgtggttcgg tctcatatga gcgataaagc atacaaaaag 1140
gcgcatgaaa aagtctcgga agaagtaaag aaactggctt attcgtcaga tgataatgct 1200
cagttcatgc agcttcagaa atataactct gtagttgccg gacttcatga atactattgt 1260
atttctacag aagtatctca tgacttcagc agacttgcct tcagtatcaa caaacagctc 1320
agaaacagac tgaaaggcga cttatccaaa aagggacagc tcagaaatgg gttcattaaa 1380
gaaaagtatg gagcaagcag acagatgagg ttccttcatg gacgtccggt ggttccactg 1440
ggatatgtcc agtcaaagaa tgcccagcat aaaaggaaat ccatcaataa atatacggtc 1500
aaaggtcggg aacagattca taagaatctt gcaatcgaca cagctacaat gttatggctg 1560
atgagaaatc ctgtaaaggg cagaaccatt gaatatgcgg ataaccgtat ttctttgtat 1620
gcggcacagt atggtaaatg tgcagtgaca ggaattccga tgaattccca cgatattcac 1680
tgccaccata aagtaccggt aagcaatggc ggttcggatg aatacgcaaa tcttattctt 1740
gtcagcaaag cggttcatat ccttatccat gcttcttcag aacctacgat tgagaagtac 1800
ctgaaatcct taaatcttga caataaacaa atagaaaagc ttaacaaact tcgttctatg 1860
gcagaaatgc cacctattat tctt 1884
Seq ID 55
gtgacagaaa aagcacgcga tttatttgat aaactgatga cgcaaattca gttagatgac 60
acagcgaaag aacatccctt aattcaaaat ggccggattg acaaggtcat tgttcaccaa 120
caaagtcgct tatgggaatt tcatttaagt tttgatgaat tgatgccagt gatgctatat 180
caaacattta tgcaacaatt ggaactagct tttcaacaaa ttgcgcaggt ttccgttcaa 240
ataaccacaa atcagacaac atttacagag gaacaattaa cggattattg gcaattggcc 300
ttgttaaata gtcaatgcaa tacgccgtta gttcagaaag tcttaaaaac acagacacca 360
caatttgaag atcggaaaat tatcttacct gttgataatg aagcagttat tccttatttg 420
aagcaacaat atttaccaat tattgaggaa ctttatttct cttacgggtt tcctaaattt 480
catattgaac caaaaatgga tcaacagcaa gctgcagaag tgctgaaaaa atttgaagag 540
caaaaattag aacaagccgc agcctttcaa caacaagctg ctgaatcgct tgttaaacat 600
gaacaaatga aaaaagaaaa acaacaacaa gcgcctgcgt ttgatggtcc aattcgttta 660
ggtcggaata ttcccaatga tgaacccatt atgccaatgg gaaacatact ggaagaagaa 720
cgtcggataa cgattgaagg ctttattttt gataaagaag tgcgtgaatt gcgttctaaa 780
cgaaaaattt taattttgaa aattactgat tatacttcct ctttcgtggt gaagaaattt 840
tcgaatgggg aaaaagatga acaagttttt gatgcgattc aaccgcaaag ttggattcgt 900
gttcgtggta gtgtacaaga agataccttc atgcgtgatt tagtcatgaa tgctcaagat 960
ttgatggaag tcgcccatgc gccaagaaaa gactacgcgc cagaaggtga aaaacgtgta 1020
gaattacaca tgcatagtaa catgagtaca atggatgcga ccaataaagt cggtgacttg 1080
gtggcgcaag cggggaaatg gggtcataag gccatcgcaa ttacggatca tggcggcgcc 1140
caagcgtttc ctgatgccca tgctgcaggt aaaaaagcag gcgttaaaat tttatacggc 1200
gtggaagcca atattgttga tgatggtgta cccattgctt acaacgaaga acatattgaa 1260
ttaacagatg ctacctatgt ggtctttgac gtggaaacaa cggggctttc cgctgtttat 1320
gatacaatta ttgaattggc cgctgtcaaa atgcacaaag gaaatgtcat tgagactttt 1380
gagcaattta ttgatcctgg ccatccatta tctcaaacaa cgatcaattt aactgggatt 1440
acagatgaaa tggtgcgcgg ttcaaaatcc gaagaagaag ttttacgcat gtttaaagag 1500
ttttcagaag gaactatttt agtggcccac aacgcttctt ttgatatggg gttcttaaat 1560
acaagttatg gcaaacatgg aatacctgaa gcggctaatc ccgtcatcga tacattagaa 1620
ttatccagat tcttgtatcc acatttcaaa agccatcgtc taaatacctt atcgaaaaaa 1680
tttggcgtca acttagaaca acatcaccgc gccatctatg actcggaatc aacaggtcat 1740
ttgtgctgga ttttcttaaa agaagcgaaa gagaatcatg atatgcattt ccatgatgat 1800
ttgaatcgtc atattggcga aggagactca tacaaacgag ctcgaccttt ccatgcaacg 1860
attttagcaa caactcaagc aggattaaaa aatcttttta aattgatttc catgtccaat 1920
gtagattatt ttttccgagt accacggatt cctcgttcgc aattatcgaa gttaagagaa 1980
ggacttttaa tcggttccgc atgttcaaac ggcgaaattt ttgaagcaat gatgcaaaaa 2040
ggtgtcgaag aagcaaaaaa tcgtgccaaa ttctacgatt atattgaagt tatgcccaaa 2100
ccagtttacg cgccattaat tgaacaagaa ttggtgaaaa atgaagcaga tttggaagaa 2160
attattagta atctggtgaa aatcggtgac gaacttggta agctggttgt cgccactgga 2220
aatgttcatt atttaaatga agaagatgcc atctatcgga aaattttagt cgggtctatg 2280
ggcggcgcta atccgttaaa tcgccatagt ttaccaaagg ttcatttcag aaccaccgat 2340
gaaatgttaa cggaatttca atttttagga caagacatcg ctaagcgaat tgtagtagaa 2400
aatcccaaca aagtcgctga tttatgtgaa gaagttattc cagtaaaaga cgatttgtat 2460
acgccgaaaa ttcctgggtc tgaacaagag atcactgatt taagctacaa tcgtgcgcgc 2520
gaattgtatg gcgatccttt accagaaatt gtcgaaaaac ggttagaaaa ggaactgaat 2580
tcgattaacg ggaatggttt ctcggtcatt tatttaattt ctcaaaaact ggtgcataaa 2640
agtaatgaag atggctattt agttggttca cggggctctg tcggttcgag ttttgttgcg 2700
acaatgacag ggattactga agtaaatcct ttagcgccac attattattg tcctgagtgt 2760
caatattctg agttctatga agatggttcc tatggttcag ggtttgatat gcctgaaaaa 2820
gcctgtccaa aatgtggcgc tcgattgttt aaagatggtc acgatattcc ttttgaaacg 2880
ttcttagggt tccatggcga caaagttccc gatatcgatt tgaacttttc tggcgattat 2940
caagccgaag cccataacta cacgaaagtt ctttttggtg aagaatatgt ctaccgggca 3000
ggaaccattg gtacagtagc cgacaaaaca gcgtatggct ttgttaaagg atatgaaaga 3060
gatcataatt tacatttgcg tggtgctgaa attgatcgct tagccaaagg ttcaacaggg 3120
gttaaacgga cgactgggca gcatcccggc ggtattattg taattcctga ttacatggat 3180
gtttatgact ttacgccaat tcaataccct gctgatgatc aagaggcaga atggaaaacg 3240
acgcactttg atttccattc cattcacgat aatattttaa aacttgatat tctaggacat 3300
gatgatccga ccgtgattcg gatgttacaa gatttatctg ggattgagcc aaaaaccatc 3360
ccaacagacg atccagaagt gatgcgtatt tttgaaggac ctgatgttct aggtgtggat 3420
gcagagcaaa tttattctaa aacaggaact ttaggaattc ctgaatttgg gacacgtttc 3480
gttcgaggta tgttagaaca gacccatcca tcaacttttg cagaattact tcaaatttct 3540
ggactgtctc acggaaccga tgtgtggcta gggaatgcag aagaattgat tcgtcgtggc 3600
gaagccaatc ttgctgaagt aattggttgt cgggatgata tcatggttta tttaattcat 3660
gctggtttgg acagtgggat ggcctttaag attatggaaa cggtacgtaa aggacaatgg 3720
aataagattc ctgatgaact acgggatacg tatttaaatg cgatgaaaga aaacaacgtc 3780
cctgactggt atattgattc ttgttctaaa attaaatata tgttcccgaa agcccatgcg 3840
gcagcatacg tattaatggc gttgcgggtc gcttacttta aagtttattt cccaatctta 3900
tattactgtg cgtttttctc cgttcgggcg gatgacttcg acttagtggc aatgtcacaa 3960
ggaaaagaag ctgtgaaagc acgaatgaaa gaaattacgg acaaagggat ggacgcctcc 4020
actaaagaga aaaacttatt aacggtttta gaattatgta atgaaatgtt ggaacggggc 4080
tataaatttg gcatgattga tttgtacaaa tcagatgctg aaaattttgt gattgaaggg 4140
gataccttga ttgcaccttt ccgagcagtg ccaagtttag gcctcaacgt agcgaaagcc 4200
attgttgctg cgcgagaaga gcaaccgttc ttatcgaaag aagatttggc aacacgaggc 4260
aaagtttcta aaacgttaat tgaatacatg aatgaaaatg gtgtcttaaa agacttgcca 4320
gatgaaaacc aattatcatt atttgatatg tta 4353
Seq ID 56
atggaacgaa gcaatcgtaa taaaaaatct tcaaaaaaac cacttattct tggtgtttct 60
gccttggttc taatcgctgc tgccggtggc gggtattatg cttatagtca atggcaagcc 120
aaacaagaat tagccgaagc gaagaaaaca gctactacat ttttaaacgt attgtcaaaa 180
caggaatttg ataagttacc gtccgttgtt caagaagcta gcttaaagaa aaatggctat 240
gatactaaat ctgttgttga aaaataccaa gcaatttatt cagggattca agcagaagga 300
gtcaaagcta gtgatgttca agtcaaaaag gcgaaagaca atcaatacac atttacctat 360
aaattatcga tgagcacgcc tttaggcgaa atgaaagatt tgtcttatca atcaagtatc 420
gccaaaaaag gcgataccta ccaaatcgct tggaagccat ctttaatttt tccagatatg 480
tcaggaaatg ataaaatttc gattcaagta gataatgcca aacgtggaga aattgtcgat 540
cgtaatggta gtgggctagc aattaacaaa gtgtttgacg aagtgggcgt agtgcctggc 600
aaactcggtt ctggcgcaga aaaaacagcc aatatcaaag cttttagtga taaattcggc 660
gtttctgttg atgaaatcaa tcaaaagtta agccaaggat gggtccaagc agactccttt 720
gtaccaatca cagtcgcttc tgaaccagtg acagaattac caacaggggc tgcgacaaaa 780
gatacagagt cacgttatta tccgctgggg gaagcagctg cgcaattaat cgggtatacg 840
ggcaccatta ctgcagaaga tattgaaaaa aatccagagt taagtagtac gggcgtaatt 900
ggtaaaactg gcttagaacg agcgtttgat aaagaattaa gagggcaaga tggtggctca 960
ctagttattt tagatgacaa agagaatgtc aaaaaagctt tacaaaccaa agagaaaaaa 1020
gatggtcaaa cgattaaatt aacgattgac agtggcgtac aacaacaagc ttttgctata 1080
tttgacaaac gccctggtag tgcggtcatt accgatcctc aaaaaggcga tttattagca 1140
acggttagtt caccttcgta tgatcctaat aaaatggcca atggcatttc tcaaaaagaa 1200
tatgatgctt acaataacaa caaagattta ccattcacag cacgttttgc gacaggctat 1260
gcaccaggct ctacctttaa aacaattacc ggtgccattg gtttagatgc agggacattg 1320
aagccagatg aagagctaga aattaatggt ttgaaatggc aaaaagataa atcttggggc 1380
ggctattttg cgacacgtgt aaaagaagca agtccagtca atctgcgtac cgctttagtt 1440
aattcagata atatttattt tgcccaacaa acattacgga tgggagaaga caaatttaga 1500
gcgggtctta ataaattcat tttcggtgag gaactagatt taccaattgc catgacgcct 1560
gctcaaattt caaatgaaga taaatttaat tctgaaattc ttttagcaga tacgggctac 1620
ggtcaaggtc aattgttaat ttcgccaatc cagcaagcta ccatgtatag tgttttccaa 1680
aataatggaa cattagtcta tccaaaacta gttttagaca aagaaacgaa gaaaaaagac 1740
aatgtaatca gcgccaacgc agcgaataca attgccacag atcttttagg tagtgtggaa 1800
gatccttcag gttatgttta taatatgtac aatccgaact tctcattggc tgctaaaaca 1860
ggaacagctg aaataaaaga taaacaagat acagatggca aagaaaacag tttccttcta 1920
acgttagatc gtagtaacaa taaattctta acaatgatta tggttgaaaa ttcaggagaa 1980
aatggttctg caacagatat cagtaaacca ttaattgatt acttagaagc aaccattaaa 2040
Seq ID 57
atgaaaaaag tgttaatggg agttctttcc ctagggttat tattaggtgc agcaacaggt 60
tgtacaagcg atcaagaaaa agccgctggt aaaacaaaag cgtcttctga aaaaacagaa 120
gcaacatctg gtgcctctgc taatggttat acagatccaa gtgaactaaa agacagttat 180
gatgttgtta ttgttggttc aggtggtgcg gggatgaccg ctgcattaca agccaaagaa 240
gctggcatga atccagttat tttagaaaaa atgcctgttg caggtgggaa tacaattaaa 300
tcgtcttcag gaatgaatgc ttcacaaaca aaattccaag aaaaagaagg cattaaagat 360
agcaacgata aattctttga agaaacacta aaaggcggta aaggaaccaa cgatcaagaa 420
ttactacgtt attttgttga tcattcagct gaagcaattg attggttaga tacaaaagga 480
attacattga gcaatttaac gattacaggt ggaatgagcg aaaaacgcac acaccgacct 540
gctgacggat cagcaattgg gggctatcta gttgacggtt tagtacgtaa cgttcgcgaa 600
gaaaaaattc cattgtttgt tgatgctgac gtcacagact tagttgaaga aaacggtcaa 660
atcgatggcg taaaagtgaa aatgaaagat gataaagaaa aaacagttaa agcaaaagca 720
gtcgttgtca caacaggtgg tttcggtgcc aacgaaaaat taattacaca atacaaacca 780
gaattgaaaa actatgttac aacgaaccaa gaaggcacaa caggtgacgg tatccaaatg 840
attcaaaaag ttggcggtgc gttagtggat atgaaagaaa ttcaaattca cccaactgtt 900
caacaaagcg atgctttctt aattggtgaa gcagttcgtg gagaaggcgc aatcttggct 960
tctcaaaaag gagaacgttt tgttaatgag ttagatacac gcgacaaagt ttctgcagca 1020
atcaatgcct taccagaaaa atcagcttat ttagtctttg accaaggcgt tcgcgaccgt 1080
gcaaaagcca ttgatttcta tgatcaaaaa ggtttcgtgg aaaaaggtga aacgattgaa 1140
gaattagctg aaaaaattgg catgcctgcc gatacattga aagcaacgat tgatacttgg 1200
aaccaagatg ttaatgccaa agatgacaaa caatttggcc ggacaactgg aatggaagct 1260
gacttaagca cagcccctta ctacgcaatt aaaattgcac cagggattca ccacacaatg 1320
ggtggcgtga aaatcaacac gaaaacagaa gttttacgtg aagatggcac accaattaaa 1380
ggtctatatg ctgctggtga attaacaggt ggcttacatg gtcaaaaccg tatcggaggc 1440
aatgcaatcg ccgatattat catttatggt cgtcaagcag gtacgcaatc agcagaattt 1500
gcctctgctc aaaaa 1515
Seq ID 58
gtgattgttc gcggaggggg cgatctagcg acaggtgtta tccaaaaatt atggcacgtt 60
ggctttaaaa tactagtatt agaaacagaa tgtcctctgg ccattcggcg gactgtttca 120
gtttgtgatg ctatttttca gaaagaacaa agagtggaag atttggttgc tgttcgaatt 180
acttcattaa aggattgcgc gaaatgttgg cagcaaaata agctgcctgt ttttgtcgat 240
caaacagcaa gtgctattca acaattgaaa ccactgattg ttatcgatgc gattttggct 300
aagaaaaatt taggtacgca tcgaggaatg gcaccgatta cgattgcttt aggaccaggc 360
ttttcagcac cgcaagatgt cgatgttgtg attgagacaa tgcgtggcca taggttggga 420
cgtttatact ttgaaggaac agctttacct aatacaggta ttccaggtga aattggcgga 480
aaaagtgcgg aacgagttgt ccatgcccct gcttcaggac aagttaccca ccttaaaaac 540
ataggggatc tcgtgttgaa aggggaagca ttatttttaa tcgatcaggt tccagtttat 600
tcgcctctga cagggacttt gagaggattg atttcagaaa aggtaacatg ttatcaaggg 660
ttgaagtgtg cagatgtaga tcctcgacca gttgagaaag tcgattgctt gacgatctcc 720
gacaaagctc gtgcattagg gggagcggtt ttagaagcaa tttttatgat tggaagaagg 780
aaaaacgttt ta 792
Seq ID 59
atgtattcaa ttattgtaaa tggtcaaagt gaaacctgtg aaacaaacaa aaaattaatg 60
gactttttgc gggaggatct gggcctaact ggaacaaaag atggctgtaa ccaaggctct 120
tgcggtgcat gtacggtgtt agtaaatgga aaggcttcca aagcatgttt gtttactcta 180
gaaaaattag ctggtaaaga agtcacgaca atcgaaggtt taagccaacg gcaaaaagat 240
gtctatgcct atgcgtttgc aaaaacgggt gcagttcagt gcgggtattg catccctggg 300
atggttattt cggcacaagg attattgaac aagaaacccg aacccacaaa agaagacatt 360
cagaaagcta tacgaggaaa catttgtcgt tgtacaggat atgtgaaaat aatagaagcc 420
attcagttag ctgctaaaat gttttgtgaa gaagcgagca ttccagaaga acattcaaat 480
ggtaaattag gggaagactt ccaacgggtc gatgcagtgg agaaaacctt aggaacaggg 540
atttatgtag atgacattga catagaagga atgcttcatg catctgcttt gcggagtgcc 600
tatcctcgcg caaaagttct atcaatcgat agtacccaag cattggcgca tcctgactgt 660
gtggctgtgt tcacagctaa agacgttcca ggaaataata aaattggtca tttagaattt 720
atttccgatt gggatgtaat gattccagaa ggagaaataa cacggtatgt tggggatgca 780
gtagcactcg ttgtttcaaa aagaaaagaa accttaccag agataaaaaa tttagtggaa 840
gttgattacg aagaaatgat accgcttact tcttgtgaag ctgctttagc agaaggtgca 900
ccagctattc atgaaaaggg caatatcttg tctcatgaac acttggtacg tggcaatgca 960
gaggaagtgt tagaaaattc tgcgtttgta gtgacagaac attattccgt accaattaat 1020
gaacatgcct ttatggagcc cgaatgtgcg attgcacaac ctgaaggtga gggcatttta 1080
ctctttagtg cgggtcaaag tatctatgat gaacaacggg aagtggctcg gatgctaggg 1140
ctagacaagg aaaaaattca tgttcaatca aaacttgttg gtggtggttt tggtggaaaa 1200
gaagacatga gtgttcagca tcatgcatcc ttagccgctt ggttattgaa gaaaccagta 1260
aaagtactgc taagccgtga agaaagtttg atggttcatc ccaaacgcca tggcatggaa 1320
atggacttta cgaccggctg tgatgaagaa ggcaatttaa ctgcaatgaa agcagtgatt 1380
tatgcggata ctggggccta tgcttcttta ggaggaccgg tattgcaacg ggcttgtaca 1440
catgcggcag gtccctacaa gtatcaaacg attgatgtgg aagggtttgc tgtatatact 1500
aataatccgc cagcaggtgc atttcgcgga tttggtgttt gccaaacggc ttttgcaatt 1560
gaaagtaatt taaacttgtt agcagaaaaa gtcggactat caccatggga aatacgcttt 1620
aaaaatgccg tcgccccagg agatacttta ccgaatggac aattggtctc gaaaaatgca 1680
gcgttaaaag aagccttgtt agcggttaaa gacgtctatg aacaagcgga agttgctgga 1740
atttctagtt tctttaaaaa tagtggcgtg ggtgtggggc tacctgatac agggcgttgt 1800
attatttctg ttgaagaagg aaaaatacat gtccgcacaa gtgctgcttg tatcggtcaa 1860
gggatggcaa ccgttacgac gcaaattgcc tgtgaaacat taaatttacc accagaaatg 1920
attgttgcgg aagcccctga tacaagaaga acaccgaatt caggaacgac aacggcttcg 1980
aggcagtcac tcttcacagg agaagcaact agaagagcgg caatgcaaattacggtatgag 2040
cttgatatgg gacgtgcttt aagcgatttg gaaggcgaag aattttatgg ggaatattca 2100
gcgaaaacgg atccattaat taatgataaa aaaagtccag ttagtcatgc tggttatggt 2160
tatgctgctg aagtagctat tctagatgac aaaggaaaag tagctaaatt tgtggctgcg 2220
tatgatatgg ggcaagtggt gaatccacga gctgctgaag gtcaaattga aggtgggatt 2280
gcaatgggca tggggtatgc tttaactgaa aaatttgcct tagaagaagg ctatgttaaa 2340
gcaaaatatg caacgctagg attagtgaac gccactcaag taccaccaat cgaaacaatt 2400
ttggttcatg ctgacaatct tcacgaaggt ttagcttatg gaattaaagg cgtaggtgag 2460
ttagcaacga tccctaccgc accagcttta gcaggcgcat attttgcctt ggatggtcag 2520
ctccgaccaa gtctgccttt agaaaatacc ccttatcaaa agaaaaaaag a 2571
Seq ID 60
atgagtgata ttatgcatcc catctcaatt gaggctttat taaattggat ttttagtgag 60
tatcaacaag atggcacaat ttttggcatt cgtaaatttt atcatgcgga tcctacaaaa 120
acgatttcgt tatttggaga aaaaatggaa accccttgtg gtccagcagc aggtcctcat 180
acacaattag cacaaaatat tattgcggcc tatttaacag gttctcgttt ttttgaagtt 240
aagacagtcc agatactgga tggagaagat ttacctgtga gtaaaccatg tatcgcagca 300
gcagatgaat gttacaacgt tgagtggtcc acagaattgc gcgttcccca agcttatgat 360
gaatacgtca aagcatggtt cgttttgaag ttacttagta aagagttcga gctaggtgac 420
ccgaatggtt tcatttttaa tatgagtgtt ggctatgatt tggcagggat tcaatcacca 480
aaaattgatc gttatattaa cgaaatgcag aacgcagaag gaacgccgat ttgggcggaa 540
tgccaagcag ctgccaaaaa atatctatct tattttaaaa aagtagatga cttatatatc 600
gaagcaatta gtcctaaagt ctgtcattct attacgctgt cgactttaca cggttgtcca 660
tcagacgaaa ttgaacgaat tgcggcctat ttattaagtg aaaaagggct ccattccttt 720
attaaatgta atccaaccat gttaggttat gaatatgcgc gtcaaacgat ggatgagcta 780
gggtttgatt atatggtttt tgacgatcac cattttaaag aagatttaca atttgaagaa 840
gcagtaccga tgttacaacg tctgcaatta ttagctaata gtaaaaatct aagcttcgga 900
gttaaaatta cgaacacttt cccggtaacg attgcagcaa atgaattacc tggtgatgaa 960
atgtacatgt caggacgttc gctatttcct cttagtattt cattggcaca aaaattatca 1020
gaagcatttg atgggaaact acaaatttct tattctggcg gtgcagatat tttcaatagt 1080
aaagaaatat tcgatgctgg gatttggcca atcactatgg caacgactct gttgaaacct 1140
ggtggctatc aacgaatgaa tcaagttgct aatgtattaa gcgcagcaga atatcctcag 1200
atggtccatg tcaacttgga taaattagct caagttgttg agaaagctaa aacacaagcg 1260
cgttaccaaa aatcaattaa attaccagaa agtaccaaac taagaaaaac agttccgtta 1320
acagattgtt atattgctcc ttgtcgaagt gacggtggtt gtccaatcaa tcaggatatt 1380
cctgcttacc ttcgttatgt cagtgaaggt aattatttaa aggccttgca agtaattgtc 1440
gataagaatc cgttgccttt tattactgga accatttgtg cacatccttg tatgactaaa 1500
tgtacacgtc aattttatga agaatctatt catattcggg aagtgaagtt agaagcagct 1560
gaacatgctt atgatgaatt attgacgaca ctagaaaagc cacagccaaa agaaaatgca 1620
cctaaaacag ctgttgtggg tggaggccct gcagggatct ctgctgggta tttattagca 1680
cgtgaaggca tgcctgttac tgtgtttgaa aaatccgaga caattggggg cgtttgtagc 1740
cagattgtac caagatttcg aatttctatg gaatctgttc aaaaagacgt tcagttagcg 1800
gaatttatgg gagccgaatt ccgtacagga caagaggcgc catcattggc ggaattgaaa 1860
aaccaaggct atacgaatgt tatttatgcc attggtgcat ggaaacatgg ggtccttcgt 1920
ttagaatctg gtcgagcatt gaactcgtta gaatttttaa aagcaaatcg agaaaatcca 1980
acgattaatc cttacggaga acaaattgtc gtcgttggtg gcggaaatac agccatggac 2040
tgtgctcgtg cagccacaac attacctggt gttaaaaaag tctcagtggt ttatcgtcgg 2100
aataaacgca atatgccagc agatgaagaa gaactttatt tagctttaga agatggtgtt 2160
gactttttag aattactttc gccaattaaa catgaaaatc aacaattaac ttgtgaaaaa 2220
atggtcctag gtgaacgaga tgcctctgga cgccgtcgac caattggtac aggagaaatg 2280
attgatattc ctgctgatac agtgattgcc gctgttggtg aaaaagtaga tacagaattt 2340
taccaagcat taggcattca cacagacaac tatgggaaag tcgtttcaaa tcaagaaaca 2400
ttggaaacaa atataccagg tgtctatgtc attggtgacg ctaatttagg accagccaca 2460
attgttgaag caatcgctga tgcaaccaaa gcggccaata atatttgtct tgtccacaat 2520
catcactatg aaaaagataa tttaaatagt gatgtcgcat ttgttagaaa caaacggggt 2580
atccttgtga cagacgagat gtcttgtagt caagctagtc gttgcttaga atgctccaca 2640
atttgtgaat cttgtatgga tgtccgtcca aaccgagcaa atattgttgt gtatgtcgaa 2700
ggaaaaccac aaattgtcca tgttgatcgg atgtgtaacg aatgtggcaa ctgtgaaaca 2760
ttttgccctt atgcaagtgc cccttacaaa gacaaattta ccttatttaa cagtgaagct 2820
gatttttatg acagtactaa ttcaggtttc tatgttcaaa atcctgtgga taaaatttgt 2880
ttactgcgtc tatggggaac ggtgtcaaca attcaattaa cagatcaagg attagatgta 2940
ccagaagatt taattgcatt aatggtaact atgattgaag aatatgctta ttgtatgcaa 3000
gca 3003
Seq ID 61
atgacaaaac aaacaattaa agagcaaatt ttacatttca tggaaagcca aaaaaagaaa 60
agcttttcaa tggaagaaat tgcacaaggc ttgaatttag aaaaaagttc agattttaaa 120
attttagtgc aaaccattgc acaaatggaa cgagaaaagt cagttagttt caacaaaaaa 180
ggcaaagtcc tgttaccaat gaaagactta ttaatagaag gaacgtttcg tgcaaatgaa 240
cgaggctttg gttttgtaac catcgatcct gaagaaccag atgtttatat tccgaaagag 300
gcaacgaact ttgcaatgga tggagatacg gttttaatcg acgtgatcca acatgcggat 360
cctctttcag atcgcggcgc agaaggtaaa gtcaaagaaa ttaaagagcg agcagtgagc 420
caagttgtcg gagaatttgt ggcatatagt gaagaagaaa tggcagaaat gggactgtat 480
ggctacatga ttcccaaaga taagaaattg aatcagtata ctgtatcaat tgcacctgaa 540
gggattaagc cagtagatgg tagcattgtc attgctgaaa ttacctatta tccagatcaa 600
gaatatccaa cgagtatgga aggactagtc aaacaagtga ttggtcataa aaatgatcca 660
ggaatggata ttttatcaat cgtggtggct catggaattc ccacagcatt tcctgatgaa 720
gttttggctg aagcggacca agtaccagaa actattgcag aaagcgattt agtcggtcgt 780
cgggatttac gtgatcagtt gattgtgacg attgatggag aagatgcgaa agatttagat 840
gatgcagtaa cggtacaaaa gttagcaaat ggcaatttct ttttaggggt gcatattgca 900
gatgtttctt attatgtaac tgaaggaagc caattggata tggaagcgta tgaacgtggt 960
acaagtgtct atttgacaga ccgagttgtg ccaatgattc cgcagcgatt atcaaatggg 1020
atttgttcgc taaacccaca tgttccacgt ttaaccatga gttgtgagat ggaaattaca 1080
ccagaaggag aagttatttc gcatgaaatt ttccaaagtg taatccaaac aacggagcga 1140
atgacctata cagcagtcaa tgaaatttta gaagagcaaa aacctgaaac gttagaacgc 1200
tacaaagaac tagttcccat gtttaaagag atgggtgagc tgcaccatat attagaagag 1260
atgcgtatgc gccgtggtgc gatttctttt gaagatcgtg aagccaaagt cctagttgat 1320
gaaaatggtc atcccaaaga tattctttta cgcacgcgtg gtgtgggtga acgattaatt 1380
gaatccttta tgttggcagc gaatgaaact gtggcgcgtc attatcatga cttaaaactg 1440
ccatttattt atcggattca tgaacaacca aaagaagaaa aaatgcaacg tttctttgac 1500
tttgctgcag tacttggcat tcttgttaaa ggaacaaaag aaaacatttc accgaaagat 1560
ttacaaaaag ttttagagca agttgaaaat aagccggaag aagtggttat caatacgatg 1620
ttgctaagaa gtatgcaaca agcgaaatac tcagaagaca actacggaca ctatgggttg 1680
gctgctgaat attatacgca tttcacttca ccaatacggc gttatccaga tttaattgtg 1740
catcgtttga ttcgcagcta tagccaagat caatctgaaa aaaatcaaga aaaatggaac 1800
gaagcattac cagaaattgc caatcatagt tcaagtatgg aacgtcgcgc agttgatgca 1860
gagcgtgaag tggacgccat gaagaaagcc gaatttatgg tagataaagt gggagaaacg 1920
tatgacggga ttatcagctc agtcacaaaa tttggtatct ttgtggaact gcctaataca 1980
atagaaggct tgattcacgt gaacaactta aaacaagatt atttccactt tattgaaaat 2040
catatggcgt tagttggtga acggacggga atgactttga aaattggtca gaaagttcaa 2100
atccgtgttg aaaaagctga tccagaaaca agagaagttg attttgaatt gatttcagct 2160
gaggaagtcg cgccagtcga aggaccaaaa ggacgtaaaa aaggcaaagc caattcttca 2220
actcgttcaa ataatcaacg aagaaataaa aaagatgaat catttgatgg taagaaaaag 2280
aaaaataaga aaaaaggcaa aggcaaaaaa caaccttttt ataaagaagc aatgaaacaa 2340
aaaaataaaa aagggaaaaa gaagaag 2367
Seq ID 62
atgaaaaata gatattttat tctgatgatc ctaagttttt atttctttgc ttttgggatt 60
gaaacctcag cagcagaatt acgatttagt gttgaaacag aaattccaga aaaccaaata 120
gataaaacaa aaacatattt tgatttaatg atgaaaccag accaagaaca aattttgaaa 180
gttcgagcgg ccaatagtac agatgaaaat ttagtgattg atgttagtgt gaagtcggcc 240
acaacaaaca gcaacggagt gattgaatat ggtgagtcgt tgacagcttt agataaatct 300
gcaccagcag atttatcaga aatcattcaa ctgaaagacg gcggtgagag tgttgaattg 360
cctgctaaaa gtgaaaaagc agttgaattg cgagtgaaga tgcctaaaga agagttttca 420
gggcaactag cgggcgggat cacgtttagt gaaaaagtcg atgaaacgaa agacaagcaa 480
aaagaaaata caaacggctt agcaattgaa aatcgctatg cctatacagt agctgtttta 540
cttcgagaaa atgaaacagt tgtacaacca gaattgtctt tagagaaagt tgaaccgaca 600
caaagaaatg cgcgcagtgt tatttctgca acactgttaa accatgaagc agcctatctt 660
caatcaatga aagtaacggc aaatgttaaa aataagaaaa caaataacgt tattttagag 720
aaagaacaag aagatatgca aatggcgccc aattcaattt ttaatttccc tattccttat 780
gaagaaaatg aaatggaagc tgggacgtat gtattggcaa tgactgtgga aggctcagga 840
aaaaaatggc aatttacgaa agaatttacg atctcaaaag aggaagcaaa aacatttaat 900
gagaaagatg taaccgtcaa aaaaacggaa tctaaactta tttatctctt aatcggttta 960
ttgatccttt tactgataat ctgcctgttc atcattttac gtctgaaaaa acaaaaaaat 1020
aaa 1023
Seq ID 63
atgagtagga agcgaaaaat cagcttaatt agtttagtca tcattttggt ttttgtcaca 60
gtcggctcag catactttgc tgtagcgggt agctatttaa agaaaacaat tgataaaggc 120
tatgttccca taaaaaatga ttataatgaa gcgcaaaata aagatagtca atcgtttttg 180
attatggggc tagacaatac aattgaacgg aaattaggca caactaggac tgatgctatg 240
atggtgatta ccgtgaataa caagacgaag aaaataacct atttaagttt gccacgggat 300
agttttgttc aaattgatgc gaaaaattac caagggatgc agcgaattga agccgcctat 360
acctacgatg gaccaacagc ttctgttaac acagttgaga aattattgaa tattccaatc 420
aatcattacg ttgtgtttaa ctttttatct tttattaagt taattgatgc ggttggcggc 480
atagatgtca atgtcaagca ggcgtttgat ggtgtcacca aagacgggcc aggatccatt 540
cattttgatg cagggaaaca gcatttagat ggtacgaaag ctttatctta tgcccgtgaa 600
agacatagcg ataacgatat tatgcgtgga ttccgacaac aagaaattat tcaagcagtt 660
gaagacaagt tgaaatctgg tcaatcaatc atgaaaataa tggacattat tgattcgtta 720
aatggaaaca ttcaaactga tgtggattcc aatgaattga ctcatttagt caaagaaggt 780
ttgacttgga ccaattatga taaacaacag ctttcttttg actggcgcac ttttagtaat 840
gaagggcgca gtatggttga actataccca gatagtattg aaaatgtccg tcatcaatta 900
cgtgtgtctt taaatttaga aaagccagat gaacgagatc aagacggcta tgtcttccat 960
acgaacggtg aatttttata tcaaagtgat tataccgttc aagatgaagc agctgaggaa 1020
aacgaaatga cttccatcaa cggcaatacg tatattggtg ttcctggtaa tacacagacc 1080
ggcccgttgc catcagttaa aacggaaaat ggctttataa aa 1122
Seq ID 64
atgaaaaaaa gtcaagctgt tcaattaatt caggaacttt caaatgctaa tggtgtttca 60
ggctttgaaa cagaagtcgt tagaatcctt caacacgcaa ctgcggattt tactattcaa 120
cggctagatt ccattaaaaa tttatatctt gagaagaaaa ataatctaag tgaaggtcca 180
gtagtgcttt ttgatgctca tagtgacgaa gtgggtttta tgattcaagc aattaaagaa 240
aacggcttgt tgcgtttttt tccgttaggt ggctgggtcc ctaatacgat ttctgcacaa 300
aaagttcgga ttcgtaaccg ggaaggtacc tatctgccag gcgtagttac tagtcggccc 360
cctcatttta tgacaccaga ggaacgtcag cgaccgttaa ccattgctga tttgacgatc 420
gacattggtg ctacctcaaa agaagaagta attgaaactt acaaaatcga tcttggtgca 480
ccggtcattc cagatgtgac gtgctgttac aacgaacaga cagatctttt tttaggaaaa 540
gcttttgatt gtcgcattgg ctgtgcttgc ttagtggatg tcatggagga gttgaaagaa 600
gagacgcttc cattcaagtt agttgcgaca gtaactgcac aagaagaagt tggcgaaaga 660
ggagcactta ttgccgcaaa acaagtcaat cctgatttag ctattgtttt cgaaggctgc 720
ccggctgatg atacggcaga aacgccagag atgattcaat cagcaatggg aaaaggaccg 780
atgttacgtt attttgatgt ttctatgatt acgaatccgg aatttcaaga gtatgcacta 840
gagattgcta aaatacacaa aattcctgtt caagtttctg ttcgaagtgg tggtggaacc 900
aatggcatgg cgattacaca agttcaagga gccccgacga ttgttgtagg aattcctgtc 960
cgttacgctc acacacccca ttgctatgta gattttcaag attaccaagc ggcgaaagaa 1020
ttagtcattc aattaatcaa aaatttagat gctgacaaaa ttcaagcact ggttcagcca 1080
ttgtcaaagg agtggaacaa a 1101
Seq ID 65
atgaccacga gtcaaatgat tgccgatttt accacacttg ctattcaagc aggtggttgg 60
atggaattgg atcgcttata tcttcagaac cgtttattat caatgattgg tgaacaagaa 120
ttaggcgaag tagacatacg gcctgtggca acacccgccg ctgatttggc ggaacaattg 180
tgtcaagttg cgagtgccaa ccaacttgta aaaacagagc aacaaaaaga acagttcatg 240
gtgcaattga tggatttgtt aacaccgcca ccttctgtgg ttaatgcttt ttttgcacaa 300
cattatgcca aagagccaca agaggctacc gagtattttt accaactttg tcaaaaaaac 360
ggtacggtca ttgaacagga agaacctgtt gttttttcaa cagtctatgg agatttttta 420
gcaaataagg tccacagtga ggcgtcaaaa gcaacgctct ctgcacaaag ctatccccgc 480
tgtgaatggt gcatggcaac agaaggctac cagggaagtc agcaatttcc tgcgactacg 540
aaccatcgag tgattcgaat gaacttagat ggcgaaagct gggggttttc ctttgtaaaa 600
caggcacaat atcagcaaca aggtgtgatt gcgtttgaaa aacatcagtc agcgaaacgt 660
tcaattaaga cttttcagca attgttgaaa attgttgagg tctttcctca ttattttgct 720
gggattgatg ctgactttga acaaaatgag catgtctatt atcaaacggg tttgcaacaa 780
tttccattag ccgaagcgag catttcagaa tatgttgagt tagctaacta tcctttaatc 840
aacgcaggaa tggtgaattg gccagttgag acatttcgcc tagagggacc gaatgcgtca 900
gaagttgccc aagccgcaaa tgatattttt gagcaatggc aaatgctaaa actgccaacg 960
gatgagatac aaatcgttgc ccgacgaaaa gaacttttat atgtcatgga cttaattttt 1020
agtcgacctc aagcaaagcc ttcgttaacg ttagcagaag tccaaggttt aacgacttgg 1080
aacaaccaga aaacgcaggc acttgaaact gtcgcttcag cgtatcaaca gcgattaaaa 1140
gaagccagtg cgtttgcaga aacaagtgaa ggaaaagcgg cttttttagc aatggttgcg 1200
ccagtgactc at 1212
Seq ID 66
atgagtattt tatgggtaat caatggcatt ttgctagtta ttgtactagc gatggtattc 60
aatgaattat atttgaaaat tatggtgaaa cgttcagcaa aaatgctgac ggaagaagaa 120
tttaaagaaa caatgcgtaa agcacaagtg attgacgttc gtgagaaaga tacatttgat 180
gctgggcata ttttaggggc gcgcagcatg ccatacagca tgttaaaaac aacaattggt 240
tctcttcgta aggatcaacc tgtttatctt tatgatcaga agaaagcttt aagtatccga 300
gcagctaatc tattacgcaa aaatggctat actgatattt atattttaaa aggcggctat 360
gatggctgga ctgggaaagt gaaaaagaga aatagt 396
Seq ID 67
gtgattttta tgaatcctga tgaaccaaat ttcatttgga aagatttgaa tgcagttcgt 60
gatatggggt gcattatcga aaatgagctg tcagaggttt taccaaataa acgatatgaa 120
acgtattcga ttatcggaag aagtggtgaa tttaatgaaa cgttcaatga ttatgaaccc 180
tttgattatg aaattgaaga tgtaactatt ccatatgaaa atttagcggc agtcaaaaga 240
tggttaactg gtaaaagtaa acttattact cacaatgatg aagataaata tttagatgct 300
atttgtacaa tgagtaaacc aacttcattc aaaaatgaat ggggtgtttt ttataccttt 360
aacgttgaat ttagatgtca accgttcaaa agaaaagtaa acgaacaacc aaaagtgatt 420
aaaacaaaat caattgaaat tactgatcac ggtgatgaaa ttgcttttcc ttatatcgaa 480
attaattcaa aaggtggcga tattacgtta aacattggta gtaactcact aacgattttg 540
cgtacacaat caggaatcgt cactattgat accgaaaagg gaaaagcaat acaagaagga 600
aatccactat ttacacgcgg cagttggata aaaacgaatc ccggtcaaaa taaattaaat 660
atatcaggaa attttataga agctaagttt tggaatagga gcgcgtattt a 711
Seq ID 68
atgtctattc acattacttt tccagatggc gctgttaaac cgtttgattc tggaattaca 60
acatttgatg ttgctaaaag tattagcaac agtttagcca aaaaagcttt agctggtaaa 120
ttcaatggtg ttttaatcga tttagatcgt cctatcgtag aagatggttc gcttgaaatc 180
gtgacacctg atcatgaaga tgctttagga attttacgtc attcatcagc tcatttaatg 240
gctaatgcct tacgccgtct tttccctaac attaaatttg gcgtaggtcc tgcgattgat 300
tctgggttct attatgatac agataatgga gaatcccctg tgacagcgga agatttacct 360
gcaattgaag ccgaaatgat gaaaattgtg aaggaaaata acccaatcgt tcgtaaagaa 420
atctcacgtg cagaagcgtt agaattattt gctgatgatc cttacaaagt tgaattaatt 480
acagatttgc cagaagatga aatcatcact gtctatgatc aaggcgattt tgttgattta 540
tgtcgtggtg ttcacgtccc ttcaacagga cggattcaag tctttaaatt actttcagta 600
gctggtgctt attggcgcgg aaactctgac aatcatatga tgcaacggat ttatggcact 660
gccttttttg ataaaaaaga tttaaaagag tttatcaaaa tgcgcgagga agccaaagaa 720
cgtgaccacc gtaaattagg aaaagaatta gatttattta tggtttcaca agaagttggt 780
tcagggttac ctttctggtt accaaaaggc gcaaccattc gtcgtacaat tgaacgttat 840
attgtggaca aagaaattag cttaggttac caacatgtgt atacaccaat tatggcagat 900
gtggaattat acaaaacatc tggtcactgg gatcattacc atgaagatat gttcccacca 960
atggatatgg gtgatggcga aatgctggta ttacgtccaa tgaactgtcc acaccatatg 1020
atggtttata aaaatgacat tcatagttac cgcgaattgc caattcgaat cgctgaatta 1080
gggatgatgc accgctatga aaaatctggc gcattatcag ggttacaacg tgttcgtgaa 1140
atgactttaa acgatggcca tacttttgtt cgtcctgacc aaattaaaga cgaatttaaa 1200
cgtactttgg agttaatggt ggcagtctat gctgacttta acattacgga ttatcgtttc 1260
cgcttaagct atcgtgatcc aaataataca gacaaatatt ttgatgatga tgcgatgtgg 1320
gaaaaagcgc aagcgatgtt aaaagctgcc atggatgaat tagaattaga ttactttgaa 1380
gcagaaggcg aagctgcctt ttacggtccg aagttagatg ttcaagtaaa aacagcttta 1440
ggaatggaag aaacattatc aaccatccaa ttagacttct tattaccaga acgttttgac 1500
ttaacttatg ttggcgaaga tggtgaaaat acacatcgcc cagttgttat ccaccgtggt 1560
attgtctcaa caatggaacg atttgtggct tacttaacag aagtttacaa aggcgctttc 1620
cctacttggt tagcaccaat tcaagcaact attatcccag tttctgtaga agcgcattct 1680
gagtatgctt atgaaatcaa agaacgttta caagcacaag gcttacgtgt tgaagtcgat 1740
gatcgtaacg aaaaaatggg ctacaaaatt cgggcctctc aaacacaaaa agtaccttat 1800
caattagtgg tcggggacaa agaaatggaa gacgcaacgg tgaacgtccg tcgttatgga 1860
agcaaagaaa cgtctgtcga agatttatca attttcattg acagcatggc tgctgaagtt 1920
cacaattaca gccgt 1935
Seq ID 69
gtgtctaaca aaaaagtcgc tggaacaatc atactgaact taaacgatgg tagtaaaaaa 60
tttttgatgc atccaatagg tgaagcgatt gagtttgcga tggcaaaagt ttctgatgag 120
atgacgggtt tagctagtat gctacaatgg ttcaaagaag aggttcaatt ggacgttact 180
tcaattagct tagtagaatt aactaacgcg catatcaata aagaaaatgt tccgctgttt 240
gtcttcgaga tggatgaaca agcacttaaa aatgaaatgg ataaagacta tacatgggta 300
gcacctgcag aattaaaaac gatttggtca aaatatcata ttgaaggtgt cccaatgttt 360
Seq ID 70
atgaagaaat ttgatggcgt aatttttgat atggatgggt tgctttttga tacggagttg 60
atttactata catctactca aaaagtagca gatgcaatgg ggttaccata tagtaaagaa 120
gtctatttag actatgttgg gatttccgat gaagaagtac aagagaatta ccgacgtatt 180
tatgcatcct atggtcatga cacagtggaa gaatttatcc gccgttctta tgatgatacc 240
ttgcaagagt ttcgatctgg taacgtccct ttaaaaccag gggtggtgga attcctcgac 300
tttttagatg atcaaaaaat tcctcgatta gttgcttcaa gtaacgtccg tccagcaatt 360
gaaatgttat taagccatgc tggaattcag gatcgttttg taggtattgt ttcagcagag 420
gatgttaaac gagcaaaacc ggacccagaa atttttcaaa aagctcgtca acttttagga 480
acagaagccc cgaaaacctt gattttcgaa gactcatttc atggggtaag tgcggctcat 540
agtgcgggca ttccagtgat catggtacct gatttattgc aaccaactga agttatccaa 600
gagaaaacat tacatgtctt ggagagttta catcaagcgc cacattattt aaaa 654
Seq ID 71
atgaacgaat tagtattttt acattcacag tatatcgagg aagaacctta tacgaccgat 60
gaggttattg caaagtattc acaaattaaa agagaaagtg tttctaaact aataaaaaag 120
tatcaaaaag acctagaaga gtttggcaag gtcggatttg aaatccgagc aatggagagt 180
gggcaaaaag ctaaattcta tcaattaaac gaggaacaag cgaccttgct tatcacttat 240
ctagataata ccgaaccagt tcgacgattt aagaaagcgc tggtgcgaca gttctatgac 300
atgaaaaacg gactctacgc tagacgtatg gaacgacaaa aggaaaaaag tgtgcgtaag 360
tcaatgacgg atgttatcaa ggagttaggg ctttccccgc attactacaa gcattataca 420
gaccttgttt ataagacagc attaggtttt aatgctaagc aattaagaga agctcgagag 480
gtaagtaaaa aatcaacgat attagatttt ttaacgtcag aagagatcga agcagtgaac 540
aaacgagagc aacaagtagc tacattgctt actcttaaaa tggattatga cacgattaag 600
agcattttga atggtcaagg tgttttatat caaacaacgc ttaaaatgcc agtagcgaca 660
aat 663
Seq ID 72
atgccaaaac caggcgaaac aattcacgcc attgaacatt ttacagcagg tggtggtaaa 60
ggagcaaacc aagcagttgc agcgaaacgt tcaggcgcag aaacatattt tattggtgct 120
gtgggaaatg atggcgctgg agctatgatg actgatttaa tgagtcaaga tgaaattaat 180
ttaactgggg tcaccacttt agagaaaaca gctactggcc aagcctttat tatggtagat 240
aacgctggtg aaaatagcat tatgatttac gcaggagcca ataacgcctt tacgccaaaa 300
caagtccagg aacatcaaga aattattgaa aaaagtgatt ttgtgattgc ccaatttgaa 360
agtgcgattg atagtacgat tgcagcgttt aaaattgcta aaaaagcagg cgtcaaaacg 420
attttaaatc ccgcacctgc gttagaacaa gttcctgaag aattactaaa cgtgacagat 480
atgattgtac caaatgaaac agaaaccgaa attttaacag gcattaaaat cacagatgaa 540
gcgagtatgc gtaaagccgc agaagcactt catcaattag ggattgaagc agtaattatt 600
acagtaggta gtaaaggcgc cttttatgac gtcaatggac gaagtggtat tgtgcctgct 660
tttaaagtga aagcggttga tacaacggct gctggcgata cttttattgg cgcattaagt 720
agtatattag aaaaagattt tagcaatttg gaagaagcta ttcgttatgg aaacaaggcg 780
tcttcgttga ctgttcaacg ttttggagcc caaccttcga ttccttatca acacgaattg 840
gcagacaaa 849
Seq ID 75
atgattaagc tagcccatat tcataaatat tattattccg aagaagaaac actgcatgtg 60
ttagatgata ttaacctcca agtagacgct ggagaatttt tagcgattat gggtccttct 120
ggttctggga aatcgacttt gattaatttg ctaggcttta ttgataaaaa gtttgaagga 180
acgtatttat ttgaagaccg tgaaattggc gacttttctg ataaggaact atcccgaatt 240
agaaatgaag cagtcggctt tgtctttcag aattttagtt taattgaaac actaacagtt 300
gaagaaaaca ttgaattacc tcttttatat agtgggttaa ccccaaaaga agccaaagat 360
cgtgtccacg aagttctgac aaaagtcggc ttgccagata aaggcaagaa gcatccaaaa 420
caactttcgg gcggacaaca acaacgtgtt gccattgcgc gtgcgattgt caatcggcca 480
agttttatta ttgcagatga accaactggg gctttagata gtaaaacatc ggaagaaatt 540
ttaacgctct ttcagcaatt aaacaatgaa ggtgtcacca ttattttagt gacacatgat 600
gaagaaacaa ttgaatactg caatcgtttg attaaagttc gtgatggaaa gattttagag 660
gaggtgctga ca 672
Seq ID 74
atgaaaaagt caggccagaa gaagcgaaat aaaaaagtgt ggttcggtat tggtgccgca 60
gtggtcgtcg tcggatttat cggagcaaaa acagtttttt cttcaaaaga agttgaacct 120
gaatacacaa cgtataccat tacagaaatg gcctcattaa aattagatgg tcaagtgagt 180
tttctagata ctcgggatat tttctttgat ccttctttag gaaaaattgc cgaaataaat 240
gttgaaaatg gtaaagaagt caagaaagat agtccattgt taacgtacaa taactcagac 300
atccaagcca cagaaacaga acaagcgaat gctgttaatc ggaacaatct tcaagttcaa 360
caagcacaag agaacgttaa cttagccact caaaaataca atgaagcgtt aaacaaagtt 420
gctgcagcca agcaaaaatt aaacaccgca aaagaggctg aagagaaaga aacactaaat 480
gcggaaattc agcaattgaa tgaagccgtt agtgctgcga atagtgaagt cgcccaagct 540
aaccaagccc ttcaattagc caacagtgat gccgtcggag cagcaaacac gcttgaacaa 600
acacgtggca aagtcaacac agtcgttaca gcaccaattg atggccaagt cactgttgat 660
gctagtgcca tgagtagtac cgacaaacca gtcattaaaa ttgcaacaca aaagaaaaat 720
attcaaggaa aagtaaccga gtatgattac gataaattac agacagggga agaagtaaca 780
gtgacgaccg ttggttcagg taaatcagca ccaggcaaaa ttgtttccat tgcgcaaacc 840
ccaatagcta aaaatgaagg caatccagta gtgagttatc aatttacagt agaaggtgat 900
tttccttggg cagaaggctt gtctactagt atcgctgtgc cacaaaagca aatgattata 960
ccgacagcag caatccaaaa agaaggccaa aaagaatttg tctatgttta caaagcgggc 1020
aaagcgaaaa aaacaccgat tgaaacagaa acaaatttag gtcgtaaagt tgtcaaaagt 1080
ggcttgaatt ggaaagacca agtgattgcc aatcccaata aagaattaaa agataatcaa 1140
gatgttcagg tagctgccaa tgat 1164
Seq ID 75
atgattaaaa aaataatagt cgttgttgct ttcatgctta caggcttctc gctaactgcg 60
atgagtgcat ctgcagaaga aataactgat ttatttttac aaaaagaagt gacatattct 120
ggtgtagaag gaggaaaaat tggagagaat tggaaatacc ctcaatttgt tggcgaaaaa 180
gctgtcgatg gcgatgaaac aacacgctgg tccgctgata agcaagatga acaatggtta 240
attgttgatt tgggtgaagt aaaaaacatt ggcgaactcg ttttacagtt gcatgcagaa 300
agtcctgtgt atgaaatctt agtttcaaca gatggtgaaa gctatcaatc tatctttaaa 360
gaagagaatg ggaaaggtgg ccaacctact aaaaaatata ttgatggcaa taatgtacaa 420
gcacgttttg taaaatatca gcagatgaaa atgtggcaac acacaaataa gcaattttac 480
agttcaagta ttatttcgtt tgaagcatat gaaaaaaaac gactgccaga agcgattaaa 540
cttttaacag agaacctgac tattagtgaa aaaagaaagc aacagctagc ctttgaagtg 600
tcaccagctg gagtagatat cacagaggat cagattgagt ggtcgagtag tgatcctacc 660
attgtgacgg ttgaccaaac gggtaattta acagcagtta agagtggtga agcgaaggta 720
acagtcaaaa ttaaaggaac agaaattagt gatacaattc ctgtgactgt tgtagcagaa 780
aataaacaat atgcggaaat gcgagcaaaa tggaaaatgc gattgttagg tacaacgcag 840
tatgataacg atgcagatgt gcaacagtat cgtgcccaga ttgctacgga gagtttagca 900
ttatggcaaa cgcttaatca agcagcagat cgtgagtatc tgtgggaacg aaaaccatcg 960
gatacagtgt ctgctgatta cacgactcaa tttaccaata ttaaaaaatt agcgttaggc 1020
tactatgaac catcaagtga gctttttgaa aaaccagaag tttatgatgc gattgttaaa 1080
ggcattgaat ttatgattga tacaaaaaaa tacaatggaa cgtattacac aggtaactgg 1140
tgggattggc aaattggttc cgcgcagccg ctaacagata cattgatttt attacatgat 1200
gacctattga atacagatgc agaaaaatta aataaattta ctgctccgct gatgctgtat 1260
gcaaaagatc caaacataca atggccaatt tatcgtgcaa caggagctaa cttaacagat 1320
atttcaatca ccgttttagg tactggactt ttgttagaag ataatcaacg cctagtacaa 1380
gtacaagaag ctgttccgtc cgttttaaaa agtgtttcct ctggtgatgg cttatatcct 1440
gatggttcct tgattcaaca tggttatttt ccgtacaacg gcagttacgg gaatgagttg 1500
ctaaaagggt ttggacgaat tcagactatt ttacaaggtt ccgactggga gatgaatgac 1560
cctaacatta gtaatttatt taatgttgtg gataaaggtt acttacaatt gatggtaaat 1620
ggaaaaatgc catcgatggt ttctggtaga agtatttcca gagcgccaga aacgaatcct 1680
tttactacag agtttgaatc gggtaaagaa acaatagcta atttaacctt aattgcaaaa 1740
tttgcaccag aaaatttaag aaatgacatt tatacatcta tccaaacgtg gcttcaacaa 1800
agtgggtcat actatcattt ctttaaaaaa ccaagagatt ttgaagcgtt aattgacttg 1860
aaaaatgtag tgaatagtgc gtcacctgcc caagcgacac caatgcaatc tttaaatgta 1920
tatggttcga tggatcgagt cctacagaaa aataacgaat atgcggtggg gatcagtatg 1980
tattcacaac gtgtcggaaa ctatgaattt gggaatacgg aaaataaaaa aggctggcat 2040
acagcagacg gcatgcttta tttatacaat caagactttg ctcagtttga tgaaggatac 2100
tgggcaacga tcgatccata tcgattacca ggaacgacag ttgacacaag agaattggca 2160
aatggtgctt atacagggaa acgcagtccc cagtcatggg taggtggctc aaataatgga 2220
caggttgcct ctataggaat gtttttagat aaaagtaatg aaggaatgaa cttagttgct 2280
aaaaaatctt ggttcttatt agatggtcaa atcattaatt tgggaagtgg cattactggt 2340
acgacagatg cttcgattga aacaatcctc gataatcgga tgattcatcc acaggaagtg 2400
aagcttaacc aaggttcaga caaagataat tcttggatta gtttaagcgc agcgaatcca 2460
ttgaataaca ttggctatgt ttttcctaat tcaatgaata cgcttgatgt tcaaatagaa 2520
gaacgctctg gtcgctacgg agatattaac gaatactttg ttaatgataa aacctataca 2580
aatacatttg ctaaaattag taaaaattat ggcaagactg ttgaaaatgg tacttacgaa 2640
tatttaacag tggttgggaa aacgaatgaa gaaatcgcag ctctttctaa aaacaaaggc 2700
tatactgttc tagaaaatac agcaaactta caagccattg aagcaggtaa ttatgtcatg 2760
atgaatacat ggaataatga ccaagaaatt gcaggactgt atgcgtatga tccaatgtcg 2820
gttatttcag aaaaaattga taacggtgtt tatcgcttaa ctcttgcgaa tcctttacaa 2880
aataatgcat ccgtttctat tgaatttgat aagggcattc ttgaagtagt cgcagcggac 2940
ccagaaattt ctgttgacca aaatattatc actttaaata gtgcggggtt aaatggcagc 3000
tcgcgttcaa tcattgttaa aacaactcct gaagtaacga aagaagcgtt agaaaaatta 3060
attcaggaac aaaaagaaca ccaagaaaaa gactacaccg caagcagctg gaaagtctac 3120
agcgaagcat tgaaacaagc acaaactgtg gcagatcaaa caacagcaac gcaagcagaa 3180
gtagaccaag cagaaacaga gttacgttcg gcagtgaagc aattggtaaa agtgccaact 3240
aaagaagtag ataaaaccaa cttgttgaaa atcatcaaag aaaacgagaa acaccaagaa 3300
aaagactaca ccgcaagcag ctggaaagtc tacagcgaag cattgaaaca agcacaaact 3360
gtggcagatc aaacaacagc gacacaagca gaagtagacc aagcagaagc aaaactacgt 3420
tcggcggtga agcaattggt aaaagtgcca actaaagaag tagataaaac caacttgttg 3480
aaaatcatca aagaaaacga gaaacaccaa gaaaaagact acaccgcaag cagctggaaa 3540
gtctacagcg aagcattgaa acaagcacaa actgtggcag atcaaacaac agcaacgcaa 3600
gcagaagtag accaagcaga aacagagtta cgttcggcag tgaagcaatt ggtaaaagtg 3660
ccaactaaag aagtagataa aaccaacttg ttgaaaatca tcaaagaaaa cgagaaacac 3720
caagaaaaag actacaccgc aagcagttgg aaagtctaca gtgaagcatt gaagcaagcg 3780
caaactgtgg cagatcaaac aacagcaacg caagcagaag tagaccaagc agaagcaaaa 3840
ctacgttcgg cagtgaagcg attaacattg aaaaatagtg gggaaaataa aaaggagcaa 3900
aaaaatgggg ggaataatgg acacttaaat actagtacag gagttgatca aactggtacg 3960
aaacaagtta agccatcaag ccaaggtggt ttcagaaaag ctagccaatt tttaccgagc 4020
acaggagaaa agaaatcgat cgcgcttgtg attattggtc ttctagttat cgccagtggg 4080
tgtcttttag tttttcgtaa aagtaaatcg aagaag 4116
Seq ID 76
atgaagaaat ctgttttatt tacttcatta cttgtattat caagcttagc tttagcggcc 60
tgcggcggtg gcagtgacga taaaggagct agcaacggcg gcagcgacaa ccaagtatac 120
acaatggttg aatcccaaga aatgcctagt gccgatccgt cccttgcgac agatgaagtg 180
agttttacca ctttaaataa tgtctacgaa ggaatctatc gtttagataa agacaacaaa 240
cccgcgcctg ctggtgcagc cgaaaaagcg actgtttcag aagacggttt agtttacaaa 300
gttaaattac gtgaagaatc aaaatggtct gatggcaaac cagttactgc tgcagattac 360
gtttacggtt ggcaacgaac agtggatcct gccactgctt cagaatatgc ctacatgttt 420
gaaccagtaa aaaatgctga aaaaatttct aaaggggaac tacctaaaga agaattgggc 480
attaaagcaa tcaatgatca tgaattagaa atcactctag aaacagcaac accatatttt 540
gacgatttat tggctttccc ttctttcttg ccgcaacgtc aagatatcgt tgaacgtttt 600
ggtaaagatt atacaaagag tagcgataaa gcagtctaca atggtccctt tacgctaact 660
gagtttgatg gtcccggaac agatactaaa tggtctctaa ctaaaaatga agaatattgg 720
gacaaagaga cggtcaagtt agataaagtc gctatcaacg tggtgaaaga agccccaact 780
gccttaaatc tttatgaaac tggtgaagtg gatgatacgt atttatctgg cgaactggct 840
caacaaatgc aaaactcgcc tgacttggtc caattaaaag ccgcttcttc tttctattta 900
gaaatgaatc aagcagatga aaaatcacca ttgactaatg caaacttacg tcgtgctatg 960
tcttatgcca tcgatcgcga ttcattagct aaaaatattt tagctaatgg ctctcttcct 1020
tcacaaggct tcgttcctgt ggatgtcgcg aaatcaccaa aaacgggtga agactttgtt 1080
aaagaagccg gcagcgacaa attagtcaaa tacgacaaga aaaaagctgt ggaatactgg 1140
aacaaagcga aacaagaact tggtgtttcc aacttaacgg ttgatttaat ggtagacgac 1200
tctgaaggcg ctaaaaaaat gggcgaatat cttcaaggat cactatctga tactttggaa 1260
ggcttaaaag taactgtgac gcctgtccct atggctgttc gcttagatcg taccttaaaa 1320
ggggatttcc aaatcgctgt tcgtggttgg agtgccgact attcagaccc aattaacttc 1380
ttagatttat tagaaagctc aacttctaat aaccgtggac gttacagcaa tcctgaatac 1440
gataaattca ttgctgcgtc caaaaccaca gatgttaacg atcctgaaaa acgctgggaa 1500
gatctaatca acgctgaaaa aacagtaatt gctgatatgg gtgttgtgcc aatttaccaa 1560
aaagcagaat cacacttacg tgcaccaaac gtgaaagaaa ttatttatca tccaacaggt 1620
gctaaatacg acttcaagtg ggcgtataaa gaa 1653
Seq ID 77
atgtcaaaaa aaccatcagg ttaccaaaaa aaagttctca cggtgttgtt ggtatcgttt 60
ggctttggcg ggttgttagc gcagttttcg ccgatgaaat caggtgggtt ttcaagttgg 120
tttgatgatt catatgtcaa agctagtgct gaatcaagta aaacaaaaga accagcacca 180
gttaaaattg agaaaaaagt caaaccttta agctatggac aacaggtcaa tcaagaaatt 240
gaaaagaagc aatatgatgg acatctggat ttgccgttag aattgcagac agatgctaaa 300
tggaaagaca ccgcatacgg atttggcaat gtggataagc cgaatacaat cgaaattaat 360
ggctgtgcga ttgtatcgct tgcaatggtt ggttcataca tggatcacca agaagttacc 420
cctctggatg tgttagcttg ggcaaaaaat gacttcttta tggaagggca agggacggcg 400
tggtctattt ttagtgcata tgctgaaatg aaaggctata actgtcaaga aattggggat 540
attgaaacag tggcagcttt cttgaaggaa ggtcatccag tcattatttc tgtaaaaccg 600
ggctatttca ctacaactgg tcacattatg gtgatgagtg gtgtggatga aaaaggcgat 660
ttctggatta atgatccaaa cgattcagaa gaaaagggcc attcaaaacg gacatttaca 720
gccgaagaag tgatgaatga agcgttaaac ttctgggcat tttat 765
Seq ID 78
ttgaagaaaa gtgtattatc ggctctaatg gtatgttcca ttacattaac aagcgtagcg 60
ttgccatccg cagcatttgc agatgaatac gatacaaaga ttcaacaaca agatcaaaaa 120
attaatgcgt taactagcca aatgtcagat gcagaagcaa aagttgccgc gattgaaaat 180
gatatggttg aaacggccaa acaaatcgat acattaacag ctaaaaagaa caagctatca 240
tcagaagtat ctaaattata tagtgaaatt tctgatttga atgtccgtat tcaaaaacgt 300
gaagtacaaa tgacaaaaca agcacgcgat gtccaagtga atggtcaaag tgattcaatt 360
attgatgctg tcttagatgc agattcagta gcagatgcaa ttggtcgcgt tcaagcggtc 420
tcaacaatga tgagcgccaa taatgaatta ctagaacaac aaaaagaaga caaagcgact 480
gttgaaaaga aaacaaagaa tgttgaaaaa caaattgctg aattagaagc agcaacaaaa 540
gaattaaatg ataaaacaga atcattaaaa acattgaaga ttcaacaaga agtggctaaa 600
aatgatttag aagcacaacg ttctgaagaa caagggaaaa aagacggctt cattaaacag 660
aaaaaagaag cggaaaaacg tttagcagaa gaacaagcac gtcaacgtgc agctgctaaa 720
aaagcagaag aacaagcggc agcgcaagca caagccgcag cacaaaaagc ggcagcagag 780
caagcgaaag caacaaaagc agccaatgag gcagcagcat ccgccgctga agaaaaagcc 840
gcaacaccag tagtagaatc atcaacaact actgaaagta ccacaacgca agaaacaaca 900
acttcaagta ctgaaacaga aagtgttgta acaacacctg tggcagcagc acctgaaaaa 960
gaaaaagaag ttcctgtaac taacccaacg actcctgaaa aaggcaatga agcaaaacca 1020
gggaatggtg gcgtgacttc aggaaaacaa gccgcaatta atgcagcttt agcagatgtt 1080
ggtaattctt atgcaacagg ttggaatcaa ccaggcgaat gtttagtatc tgttcgtcgc 1140
tggttagcgg caggcggtat taacttcggt tatggtggtc caaacagtgg ctatgttgct 1200
tctggcgcaa cacaagttag ctggagtaac gtgcaaccag gcgatgttgt tcaatatgaa 1260
agtgcctata gtccagatag ctggattggt ggcgtgcata ctgtcttggt tacaggtgta 1320
agtggtggaa gtgttcaaat cgttgaagcg aacaacccag gaggttctgg ttatgtttct 1380
tctaactcta actggtcacc agcaccacca gcaggattcc gcgctgttgt atggcgtttt 1440
ccaggc 1446
Seq ID 79
atgtcctcat tattaaaacg attggttcag ttggttttgt tagtcgtcgc tgtcttgctg 60
attcgacact atgttttctc ccctgctgcg gtgaacggct cttcaatgga accaacactt 120
cataacaacg accgtttatg ggtgacctcg attaaaaaac cacagcgctt tgatattatc 180
gctttcccta gtcctcgcaa cggccaacga gtagccaaac gtttaattgg tttacctggc 240
gaaacagtcg agtatcgcga tgataccctt tatattaatg gtgtatcact cagtgaagat 300
tacttagcaa gtgctaaacg aaatgtctct aaaaatgaaa attataccca agattttacg 360
ctagagacct tagaagccac ccaatccctg accgttccag aaggcatgta ttttgtcttg 420
ggggataatc gcccgcgctc agacgacagt cgttattttg gctttgttaa acaagcgagt 480
gtggaaggtg ttttgacttt tcgttattat ccattagata aaattggctt tcca 534
Seq ID 80
atggcgaatg atgacttaca acaacgtctg gataaaggaa tgtacggtac gcccctcgtt 60
aatcctgaag aacaacacaa atacatgggc acatttcgtg aacgctgccg tctctccatg 120
accgtcgctg aaatgaaaga tgcacagaac caaaaacatt tattagaaga attagcgaag 180
catcctgaag ttacagtact tttaaatggc gaaatctctt ctgacctgca aagtacctat 240
attaaattat taaatcagca tggcgcaaac tttaagattg ttaacaattt tgtggagaac 300
aaccctgact ctctcggctt actgttagct gaaaaacatg cagtcgatga acctgtgatt 360
gacgttacag aaaaatatcc tcaagcaaca gaaacgccaa aagaagagcc aacagctaaa 420
aaaagctttt ggcaaaaact atttcactca 450
Seq ID 81
gtgtatttaa aacgaattga aattacagga tttaaatcat ttgcagataa aaccattatt 60
gaatttgaag atgatgtgac agcagtggtt ggtccgaatg gaagtgggaa aagtaatatc 120
acggaagccg ttcgctgggt attaggtgaa caatctgcga aaaaccttcg cggtggcaaa 180
atgaacgaca ttatctttgc cggttcggaa ggtcgtaagc cgctaaatat tgcggaagtt 240
accgtgacgt tagacaacag cgaccattat ttagcattgg attatagtga gattagtgtc 300
acccgtcxtt taaaacgaac gggcgaaagt gactttttta ttaataaaca agcgtgccgt 360
ttaaaagaca ttcaagattt atttatggat tcagggttag gcaaagaatc tttttcaatt 420
atttcacaag ggaaagttga agcgattttt aatagtaaac ctgaagaccg tcgagggatt 480
tttgaagaag cagcgggcgt tttaaaatac aaacaacgaa agaaaaaggc tgaacaaaag 540
ctttttgaaa ccgaggacaa cttaagccgt gtccaagata tcatttacga gttggaagac 600
caattagttc ctctagccgc ccaagcagat gcggctaaaa agtatttagc tttgaaagaa 660
gaacttaccg aaattgatgt caatttaact gtgaccgaaa ttcaagaagc aaaagctatt 720
tgggaaacga aaacgcaaga attaacagcc attgaagaaa agttagctgg cgcaagtaag 780
caagtccatg acttagaagg caaattggta cgtttacgca gcaagcgcaa tcgtttagat 840
gaacaaattg aaacggaaca acaacaatta ttacaagtga cagaagcgtt gaaacaagca 900
gaaggacaaa aaaatgtttt aattgaacgt tccaaacaca cctcccaaac ggcaagcgaa 960
tacgaagaga cattggcaga aacggccgaa aaaattgttc gttatcgaga agaattgcag 1020
acgctggaaa cagcaatcgc agaaaaaacg gcacaacgtc agacgttaaa agaagcattg 1080
gctttggcaa cgaaagatgt ggaaaaatat agtaagtctt ctaaagaatt aatggaagaa 1140
ttgcgcagtc agtatgtgga agtcatgcaa gaacaagcca atacagccaa tgacttaaaa 1200
tatttagaac gacaatatca acaagaaaca gctaaaaatc aacaatCgtt agcaaaacac 1260
gaagcgctag aagaacagat ggttgaagct ctcgcaatga aagagacgct agaaaaagag 1320
caaaaagttg caaaacaagg cttacaagaa cagttggaag aatacactgc gttgaaagca 1380
acgcttgaag ctaagcgtga acgattagca cagcgtcaaa acgatatgta ccaagcgatg 1440
aatcaagtgc aacaagcgaa ggcacgccaa aaaagtttgc aagaaattca agaaaattac 1500
gctggcttct atcaaggcgt gaaagctgta ttgcgtcaca aaaaccaatt aactgggatt 1560
gttggcgcag tggctgagtt aattgaagtg cctaaagaat atacgttggc gattgaaacg 1620
gccctaggtg gtgcggcgca acatattgtt gtggaaaatg agaaagatgg tcgagcaggc 1680
atcaccttct taaaacaaca acacagtggc cgagcaacct ttttaccatt gactaccatt 1740
aagccacgct ctgtatcggc gatggttcag aatcgtttgg ctggtgcacc gggcttcgtg 1800
gggattgcca gtgaactagt tcgttatcca gaacaagtac aaaccgttat tcaaaatctc 1860
ttaggcgtca cgattttagc ggcagattta acgagtgcca accagttagc aaaattagtc 1920
aattaccaat atcgcgttgt ctcattggaa ggcgatgtga tgaatcctgg cggttcgatg 1980
actgggggag ccaataaacg tggcaaccaa ggaagcctat tttcacaagc gcaagaactt 2040
caaacaatta ctgaacaaat gactcaatta gaaacacaac tgagaagtgt agaacaagaa 2100
gtccaagcgc tctcgcaaga agtgaaaaca gccacggaac gtgcagaaat gttgcgctct 2160
gctggtgaac aaaatcgctt aaaacaacaa gaaattgaca ataaattagc caatcaaaca 2220
gaaacgatta ctcgtttaac aaaagaaaaa cgcttgtttg aatatgaatc acgggaattg 2280
catcaattct taaccgaata tcaaacgaaa aaagccacat tgacagagca acaagcaaac 2340
ttaacggcaa cgaaagaacg tttagatgct gaaatgaaac aagtggaaca agaagctagc 2400
caaatggaaa cctttaaagc ccaagcgcaa gaacgcttga cgacagttca agcagaacaa 2460
gcagtagcgg cggaacaatg tgcccatttt gctcgtcaaa agcaagacaa acaagaacaa 2520
ttggacgaat tgttgattcg agaaacggcc atccgtcaac agctacaaca attaagtagc 2580
cattctagcg atcaccagtt aaccgaagaa ggcttagcgg cacaagtcgc tcaattagcg 2640
gagaaacaaa cagcgttgca aacctcttta caaacggcac gtagtcaacg acaagcgttg 2700
caagaagaag tagacgagtt agatacaaaa ttagcggaag aaaacaaacg ccaacaacag 2760
tatttagcag ataaaacaca aatcgaagtc ctaaaaaatc gtgcagaaat gcaattagat 2820
agctcgttaa gttacttaca ggaagaatac agcttaacct ttgaagctgc ctatgaagcg 2880
tatttcccaa tcgatgattt agcgcaagcg cagcaaacag tgaaacgcct aaaacaagaa 2940
attgagcggt taggacctgt taacttaagt gccatcgagc aatttgaaca agtcgatgag 3000
cgccatcaat ttttagtcag tcaacgagat gatttattaaa tgcgaaaga acaattattt 3060
gaaaccatgg atgaaatgga ccaagaagtg aaagaacggt ttaaagaagt ctttgaagca 3120
attcgtggcc agtttaaagt ggtatttcct aatatgtttg gtggcggacg ggcagaactg 3180
gtgctaacca acccagagga tctcttgaat acagggattg agattgaggc gcaaccacca 3240
gggaaaaaat tacaacattt aagcttgttg tctggaggcg aacgggcatt aacggcgatt 3300
gcgttactct tttcaattat tcgtgttcga ccagttccgt tctgtatttt agatgaagta 3360
gaagcagctt tagatgaagc caacgttgct cgttttggcc attatttaag cgaatttgaa 3420
gatggtacac agtttattgt ggtgacgcat cgtaaaggga caatggaagc cgcagatgtt 3480
ttatatggcg tgactatgca agaatctggg gtttcaaaaa tcgtttcagt tcgcttggaa 3540
gaggtcaaag aaggtggcgc aattgaaaaa agcaat 3576
Seq ID 82
atgcaaaaag tagccaaagt tattgtggat gtaccaacga tgcaaaccga tcaaccattc 60
acttatttag ttcctgaaaa tttaaatgag caattggctg tcggcatgcg tgtagaagtg 120
ccttttggta acggtaatcg gcatgttcaa gggtttgttc tagccattga accagtggct 180
gcaacggttc ttgacgaaac caatgttcag ttgaaagaac tagtggcggt gttggattta 240
aaacctgttt taaatacaga aatgttggct ttagctgatt atatgaagga aaaaacgttt 300
gcttttaaaa tcacttgttt gcaaaccatg ttgcctagcg taatgcgtgc cgattaccaa 360
aaatatattt atttaacgga tgagctttct gaagaattac aagatcagtt attttatggt 420
ttagaggaaa tttcgtggga ccaagcgcaa gaacgtgggc ttttgcctca actaatggcg 480
ttgcgtaaac agcaaaaagt cgatattcgc tacgaagtta cgacgagaaa taaagtcaaa 540
atggtccgct tcattcaagc cgctaaggaa tttgaacaac tagaggaaat tcgtttgggg 600
ttacgcaagg gagctaagaa gaaagagcaa cttctttact atttacaacg cttgggcact 660
gaaaaagtca ccgctgtgaa ggaaatgaaa gaattaggtt tcagtaccgc gcttttaaat 720
gaagcggcca aaaacggctg gttaacgttc attgaaaaag aagcgtatcg tgatccgttt 780
gctaatcaga cgtttgaaaa aacgactgcg ttatctttga atgcagaaca acaggtggct 840
gtggaaacaa tcttacaatc ggtccaagaa cagcaaagcc aaacctactt attggaaggt 900
attacaggaa gcgggaaaac agaagtttat ctacaggtca ttgcagaagt tcttaaccaa 960
ggcaaaacag ccattatgct tgtccctgaa atttctttaa ctccgcaaat ggtgcaacgc 1020
tttaaaagtc gctttggcga acatgttgct gtaatgcaca gtggcttatc gcaaggtgaa 1080
aaatacgatg agtggcggaa gattgaacga ggagaagcgg aagtggtggt tggcgcgcgt 1140
tcagccattt ttgcgccaat cgaaaacatc ggcgtgatca ttgttgatga agaacacgag 1200
gcaagttata aacaagaaga aaccccgcgc taccacgcga gagatttagc gatttggcgt 1260
tcggagtacc atcattgccc tgttgtttta ggcagtgcca cgccatcctt ggaatcacgc 1320
gctcgcgcac aaaaaaatgt ttatcagcgg ttacgattaa cacagcgtgc caatcaagcg 1380
gcaaccttgc ctacgattga tgtagtggat atgcggcaag aagtagaaaa tggcaatgtc 1440
tcttcttttt ccatgtcgtt gcaagaaaaa ctccaagaac gcttagagaa aaacgaacaa 1500
agtgttctcc tacttaatcg ccgtggctat tcgtcatttg tgatgtgtcg cgattgtggc 1560
tatgtgttgc cgtgtccgaa ttgtgatatt tccttaactt tgcatatgga tagtaaaaca 1620
atgaaatgtc attattgtgg acatgaagaa cggattcctt atcgctgtcc taactgtggc 1680
caagataaaa ttcgctacta cggaacaggc acacaaaaag tagaagaaga attacaaacg 1740
ttgttaccag acagtcgtat tctccgaatg gatgttgata cgacccgtcg caaaggtgca 1800
catgaaaaga ttttgcgaac atttggtgaa ggacaagcgg atatcttatt aggcacacaa 1860
atgattgcca aaggactgga ctttccaaat gtgacgttag tcggtgtctt aaatgctgat 1920
accgccttga atttacccga ttttcgttcc agtgaacgga ccttccaatt attgacacag 1980
gtcagcggtc gagctggtcg cgctgaaaaa ccgggggaag tcatcattca atcttttaac 2040
cccgagcatt atgcgataca attggcaaaa gcccaagatt atgaagattt ttatacaaaa 2100
gaaatgtata ttcgtcatcg tggggattat ccgccgtact attttacggt ccaaattacg 2160
gctagccatc ctgaagaaaa tgaagcagcg aaacaaatgt ttcaaatcgc gacgaaatta 2220
aaacaaggct tatcgccgca agctattttg ttgggaccaa cacccaacgc aattatgcgt 2280
gtcaataatc gatactttta tcaagtgatt atcaagtata aacaagaacc aatgcttcaa 2340
ccattattga aagaaatttt aaccgataca caacgagcaa cggctcgtgg cttaaagctt 2400
tccattgatg cagaacccat gaattttatt 2430
Seq ID 83
atggacttac atttaaccgt tgatctaaaa aaaacaaaga cactcattga cttagccgat 60
caggcccact taatcattga acaaacaact aaattaccga aaaaagaggc agcgattctt 120
ttcttgctaa tagataacca actagttgct ttaggacttg ctgaagaaaa agctacatat 180
aaagaagttt cttttgatca ctcgatttta ttgaaaccga cttgggacac agaacttgcc 240
tatttagcgc aagctttcct agatgatgcg aaagaaagtg gtttaacatt ggaagcaacc 300
ccaacgacag tcaaaattcc gaaaagtgcg gtagcgacag tgactaacta taaagaaaca 360
gtcaccttta tcttggaacg ttttggctac tctctgttta aaaaaccggc gccgaagaaa 420
gccagaccag ccaaagcacg ccacaaatgg acaaaagaag ttagccaaat tcctttttat 480
attgatactc gacaaagcaa agcaactgtt ttttggcaaa agcgcaatga aatgcttatt 540
aaagctggcg cgacaatgat gcccgaagcc cctttaaata aagacgggtc tgtcggtttc 600
tccgctagat ttggtgaaaa acttcgtgat gaacgcaagg gccaatttaa agactttgtc 660
acaacggaag atatcgtctt aaaaagcgtt aatgaagtcg gcttgtttct ctattttgct 720
ggaacaaaca gctggttaga gctagttgat gagaacggca aaaccttaaa tgaatggact 780
gtggtggaa 789
Seq ID 84
atgaacagat ggaaagtata tgcaacggta atcgcttgta tgttatttgg ctggattggc 60
gtggaggcgc acgcttctga atttaatttt gcggtcacac caacaattcc cgaaaatcaa 120
gtggataaat caaaaaccta ctttgactta aaaatggcgc ctggtgccaa acaaaccgta 180
gaaattcagt tacgcaatga tacagatgaa gacattacca ttgaaaatac ggtgaactca 240
gcgacaacaa atttaaatgg cgtagtagaa tatggccaaa acgggatcaa acctgacaaa 300
accttacgtt ttaacttaaa agattatgtg gaagcaccga aagaaatcat cttgccgaag 360
cattcccaaa agaccttacc tttaaccatt acgatgccta aagattcttt tgatggcgtg 420
atggctggcg gtataacact caaagagaaa aagaaagaaa caacgacttc tgcggatcaa 480
tcaaaagggt tagctattaa taatgaatac tcctatgttg tggctattat tcttcagcaa 540
aatgagacaa aggttcaacc agatttaaaa ttactggggg ttaaaccagg ccaagtcaac 600
gcgcgaaacg tcatcaatgt ttctttacaa aacccacaag cggcctattt aaaccaatta 660
catttaatca acactgtttc aaaaggaggc gaaacgcttt accaatccga tactgaggat 720
atgcaagtgg cgccaaactc taactttagt tacccaattt ctttaaaagg ggaacgatta 780
acgccaggaa aatatgtctt gaaatcaacg gcctatggtg taaaagatga aaagggcacc 840
tatcaagtca aaggcgccaa tggtgaagaa cggtacctgt acaaatggga atttacaaaa 900
gaatttacta tttctgggga cgtcgctaaa gaattaaatg aaaaagacgt aaccattaaa 960
ggaaccaatt ggtggttgta tctactgatt gcattaatca ttctagcgct gctcttattg 1020
attttcttct tgtatcgtaa aaagaaaaaa gaggaagaac aacaatctga gcaa 1074
Seq ID 85
atgagcaaga atttttgggc aacattgcca aagccctttt ttgttttagc accgatggaa 60
gatgtcactg atgtggtctt tcgtcatgtc gtgaaagaag ctggggcacc agatgtgttt 120
tttacagaat ttactaattc ggatagttat tgtcatcctg aaggaaaaga tagtgtacgc 180
gggcgcctcg tttttacaga agatgaacag ccgatggtgg cacatatttg gggggataaa 240
ccggaatttt tccgtgaaat gagtatcggc gtagcggaga tgggctttca agggttagac 300
ataaatatgg gctgtcctgt gcctaacgtg gctgagcgtg gaaaaggcag cgggctaatt 360
ttgcgcccag aagtcgcggc tgaattgatt gacgctgcca aagcaggtgg cttacctgtt 420
agtgtcaaaa cacgcattgg gtttactgaa atggcggaaa tggaggcgtg gatcacgcat 480
ttattagagc aagacattgc gaatctatcc attcatttgc gaacacgaaa agagatgagc 540
aaagtggatg cccattggga ggtcattccg caaattatgg ctattcggga ccgtgtcgca 600
ccgcaaacga caattacgat taatggggat attcccgatc gtcaaaaggg cctagaatta 660
gcagaacaat atggtgtaga tggcatcatg attggtcggg ggatttttaa aaatccttat 720
gcctttgaaa aagaacccaa aacacatacg ccacaagaat tgctgggctt gctacgttta 780
caattggatt tgcaagacaa atatgcggaa ttggtgcctc gctccatcgt tgggctgcat 840
cgcttcttta aaatttatgt caaaggcttt ccaggtgcca gtgatttaag agcacaatta 900
atgaatacaa aatcaacgga tgaagtgcgc cagttgttag cgacgtttga aacagaacat 960
ggtgtgcttg ac 972
Seq ID 86
atgcgttatt tagatgcaga agcaattgag aatatagcca caggagcagc ttttttaggg 60
acaggaggtg gcggtgatcc ctacattggt aaaatgatgg ctttgtccgc catcgaagaa 120
aacggacctg tcaaactggt ttctccagaa gaaattgccg cggaggattt tttcctaccc 180
gccgcaatga tgggtgcccc atctgtcgca attgaaaaat ttcccaaagg cgacgaattc 240
gtccgtgtct ttgagaagtt aggaaaatat ttagaccaag aaacgattgc gggaaccttt 300
ccaatggaag ctgggtgcgt caattcaatg attccaattg ttgttgcagc gaagctaggc 360
attcccttgg tggattgcga tggtatgggt cgggcctttc cagaattgcc tatggtaacg 420
ttccatttga atgggatgtc agcgacccct atggcaatta ccgatgaaaa aggaaatatt 480
ggcattatgg aaacgattga taatacttgg acagaacgtc ttgctcgtgt tcaaacggtt 540
gaaatgggcg ccagtgcttt agtgagcatt tatcccgcga caggcaaaca attacaagac 600
tatgggattc acaacatcgt gacattatca gaagaaattg gcaaagtgat tcgaggtacc 660
tatgcagatg aacaagaaaa acgccaagca ttagtagaag ttacggatgg ctttgaattg 720
ttccaaggaa aaattctaga tgtggaacga gaagtaaaag gtggcttcaa tttgggacgt 780
gtcaaattga gtggcttaaa cagtgacgct ggttcagaag cagtcgtcca ttttcaaaat 840
gaaaatttaa ttgccgagaa agatggtcag gtgattgcga tgacgcctga tttgatttgt 900
atggtagatt tagaaacttt aacgcctgtg acaacagaaa gcttgaaata cggcaaacgt 960
gtccaagtaa tgggcttgaa agcgaatgcc gcttggcgaa cgaaaaaagg tatcgaaaca 1020
gtgggtcctc ggtatttcgg ctacgaaatg gattatcaac cactagaaaa cttagtagca 1080
aaggaggaca aa 1092
Seq ID 87
gtgacaattg ttttattact actattaaat gtctcaatta tgttcctttt aggacaacta 60
cttagtgaaa actcgccctt agctggtgtc atctgggcca ctaaggatgc ggtcgctaaa 120
gaattaaata ctgatttgtt ttggagctgg aataaatttg tcttacccat tttcttttta 180
gtagatgcag ctgttctcta ttggcgctta attcgtcgct accatcaaat gcaattgcgt 240
catattatca gtgagttaca ttacatcgct gatggtaatt acaatcaccg cattccgttt 300
gaattaagtg gtgatttagc aaaagtcgtg acgagtatta acggtctggt ggatagtact 360
gtcgcagcca ttgaagatga acggcgaatt gaaaaatcaa aagatgaatt aattacgaat 420
gtcagtcatg atattcggac accgttaact tctattattg gttatctggg attgattgaa 480
gatcgtcaat ttcatagcca agaagattta ctgaaatata cgcataccgc ttacgtcaaa 540
gcgaaacaaa tgaaattatt agttgatgat ctttttgaat atacaaaagt ccgtcaacca 600
agtgtaccga ttcatacgac gacttttgat atggcacaat taattgaaca gcttgctgcc 660
gattttgaat tggaagcgaa aaagattaat atgcaaatcc aagtaaaagc caatccagct 720
tctttaatga tggaaggcga tacggaaaaa ctagtccgag tctttaacaa ccttctttca 780
aacgctttga aatatggcaa aggtggccat catattgtaa tggaagtaga caaagtcggc 840
accgaagcaa ttattgctgt tcgcaatgat ggaccagcta tccctaaaca ttctttagat 900
cagttgtttg accgcttcta tcgtgtcgaa gaatcccgat cacaagaaac ggggggtact 960
ggtttaggtc tggctattgc acaaagtatt gtcgctttac atggtggata tatttatgcg 1020
aagtctgatc aaaagtggac ttcctttatt attcatctac ccttacagcg taccaacaag 1080
aaatcagaga gt 1092
Seq ID 88
atggaagcag aagcattacg agcaatcgtg gctgagaatc gtcaattaga acagaatttg 60
actaaaagaa atgaacaata catttttgac ttaaaaaaat cattaaaagc ggctaattta 120
tctgaagaag agctagcatt ggctctacac ggtattttgc cagaattagt agccggtcaa 180
aaaacaggta aaacagcccg tcagttattt ggcactgttt cagaacggac ggaagcaatt 240
ttaaataaac cagcagaagt caaagaacca gcgggctgga tgatttggct cgacaatacc 300
ttactattac ttggtttatt aacaatcatg ttagcggcga tgtctctatt ttcaaaagga 360
accgcacaac ctctcggatt aaccacatat atcttaggtg cgatggctgg gggttatgtt 420
ttctatctaa tgcacaaata tgtttaccgt tttgatcgtc aaggtggcga caaaagcaaa 480
cgtcctggtt ggttaaaaac gaccgccatt ctatttggtg ggatgttctt gtggattgca 540
gttttcgctg gttcagcgat gctaccacca gtgatcaatc ccatcttaga tccgatgatt 600
gctttagtaa tcggtgcgtt agcctttgta gcacgctatt tcttcaagaa aaaatacaat 660
attcaaggta gtttcatgac acgtcaa 687
Seq ID 89
atgaatttag ttttagccga gattgccgcc atggggctga aaaacatttc gattgcacct 60
agttcaattg caaatgttca tgaacctctg attgaacaca ttaaaaatgg agtagtcacc 120
aacattactt cttctggttt gagagataaa gtaggagccg ctatttcagc aggtatcatg 180
gaaaatcctg tggtgattcg ttcacatggt ggccgggcac gggcagtagc agcaggcgat 240
atccatattg atgtagcttt cttaggtgca ccaagttcag acgcttatgg aaatgcgaat 300
ggaaccaaag gaaaagctac ctgtggttct cttggatatg cgatggtcga tgcaaaatat 360
gcggatcaag tggtcatcat tactgacaca ttagtaccat atcctaatac ccctatcagt 420
attccacaaa cagatgttga ttatgtggta gaaattgatg caattggtga tccagatgga 480
attgccaaag gcgcgacacg ttttacaaag aatcctaaag aattattgat tgctgaatat 540
gccgcaaaaa taattacaca ttctccttac tacaaagaag gcttctcatt ccaaacagga 600
acaggtggct cttcactagc agttacgcgg ttcatgagag aacaaatgct taaagatggc 660
attaaagcaa gtttcgcttt aggcggaatt acgaatgcaa tggtggaact tttggaagaa 720
ggcttagtag aaaaaattat tgacgtccaa gactttgatc atccatcagc tatttcttta 780
ggggaaaatg ccaatcatta tgaaattgat gccaatatgt atgcctcacc gcttagcaaa 840
ggggcggtta ttaatcaatt agacacagct attttatctg ctttagaagt ggacacagat 900
tttaatgtaa atgtcattac tggttcagat ggtgtcatcc gtggtgcttc tggcggacat 960
tccgatacaa gtatggcttg taaaatgagt ctagtgattg caccacttgt tcgtggacgg 1020
attccaacaa tcgttgagca agtaaataca gtcgttacac caggtacgag tgtcgatgtc 1080
gtagtaacag aagtcggcat tgcaatcaat ccaaaaagaa cagacttaat tgattgtttt 1140
aaaaccttag atgttcctca gtttacgtta gaagagttga aagacaaagc ctacaacatt 1200
gttggcaccc cggaaccaat taaatatgga gacaaagtcg ttgcgttaat tgaataccga 1260
gatggtagtc taattgatgt ggtacgcaat gtt 1293
Seq ID 90
atgacgatga atccttttaa aggaaagcaa tttcagcagg atgtgattat tgtagccgtg 60
ggctactatc ttcgttataa ccttagctat cgtgaagttc aagaaatctt atatgatcgt 120
ggcattaacg tttctcatac gacgatttat cgttgggtgc aagaatatgg caaactactc 180
tatcaaattt ggaaaaagaa aaataaacaa tccttttatt catggaaaat ggatgaaacg 240
tacatcaaaa ttaaaggaaa atggcattat ttgtatcgag ccatcgatgc agatggttta 300
accttggata tttggttacg taaaaaacgg gacacacaag cagcctatgc ttttcttaag 360
cggttagtga agcagtttga tgaaccgaag gttgtagtca cagataaagc cccctctatt 420
acaagtgcct ttaagaaact aaaagaatac ggcttttatc aagggacaga acatcgtacc 480
attaaatacc tgaataattt gattgaacaa gaccatcgtc cagtaaagag acgcaataaa 540
ttctatcgaa gtttacgcac tgcctctacc acgattaaag gcatggaagc cattcgagga 600
ttatataaga aaacccggaa agaaggcact ctcttcgggt tttcggtctg tactgaaatc 660
aaggtattat tgggaatccc agct 684
Seq ID 91
atgaataaaa aaagaattat ctttgggctt ctctcttttt tcttgcctat atttttagtc 60
tttggaggct tacttttttt tcttttatta ttaacgagta cgtcagatac ttcaaaaaat 120
gattgtattc agccaagtat aaataatcca actgatgcga cagatacacc taaatcgatc 180
gagcagtttg taaaaagcca taaagatgct taccttttat catggaaagc aggtggcttt 240
ttaccgtctg ctagtatttc tcaaacgatg gtagaaaatg ggtttaattt tactaatcca 300
tcggggacgt cattttggca ggcgcacaat atgggcggtg ttaaaacgtc aaaaaaagaa 360
gattttcctg taactttagc aacattcggc caagattctg ttgatatttc tggtacaaag 420
ccagggtcaa acgtcggtga tggcactggt ggggcatata cctggtttaa agactacaat 480
gctggaattg ttggaaaagc agaatttatg gcacaccaga cactgtatac aggtgctatc 540
aataatactg acggattaag tactttatca gctatttatt caggaggatg ggctacagac 600
cctacttacc tcatgaagtt acaggccaca tataatagct taggcaagca gtttcaatgg 660
ttggaccaag aagcaataca gaaatatggt aacgcgccgt ttaaaaagag cgaacttgtc 720
ctaaatattc ccggaaaatc cccaatcaca aacgaaaaat atggtaaaaa ttctgattgc 780
gttgttactt cagatacatc cgatcaagtt acaggacaaa atactgcccc atcattagaa 840
gtaccaagtg cgtataaagg aaagttaaca cttccgccta tcgattcgaa tgattacgct 900
ggaaataact atccttttgg acaatgtacg tggtacgcct ataatcgtat ggcgcaaatt 960
ggcaaaccaa ttgaatggtt ttcaggtgat ggcggaaatg gcgcaggttg ggctaattct 1020
gctcgtgcga aagggtacac agtagtaaaa gtaaaaccaa gtgttggttg ggcagctagt 1080
atgcaaggtg gcataggagg ttctgcacccg ccctatgggc atgtggcagt tgtagagtat 1140
gtcaattctg acggcagtat tcttgtgagt gaagcaaatg ttattaatca aggctctggt 1200
acgcgttcat ggcgagtatt agacagagca acggttgaac aaattgattt tattcaagga 1260
aagggagcg 1269
Seq ID 92
ttgaacttac aaattaaaat taaaatggag gaaataatga tgaaaaaaaa tctttttgct 60
agtttattta gtgccacact actatttggg ggaagtgaaa tttctgcttt tgcacaagaa 120
attatccctg atgatactac gacaccgccc attgaagtac caacagaacc aagtacacca 180
gaaaagccaa cagatccaac accgccaatt gagccacctg tagaccctgt agagccacct 240
attacaccaa cggagccaac agaaccgaca gagccgacaa caccaacaga acctgaaaaa 300
ccagtagttc caacggaacc gacaacacca acagaaccga caactcctac agagccaagt 360
gaaccagaac aaccaacgga gccaagtaaa ccagtagaac ctgaaaaacc agttacacca 420
agcaaaccag cagaacccga aaaacctgtg acaccaacta aaccaacaga acctgaaaaa 480
ccagtacaac cagcagaacc aagcaagcca atcgacgttg ttgtaacgcc aacaggggaa 540
ttaaatcacg ctggaaatgg tacacaacag ccaacagtcc ctattgaaac aagtaatttg 600
gcagaaatca cacacgtgcc aagtgtaaca acacctatta caactacaga cggagaaaac 660
attgtagctg tagaaaaagg tgttccactt acacaaacag cagaagggtt aaaacctatt 720
caatcgagtt acaaagtatt gcctagcgga aatgtagaag taaaaggtaa ggacggtaaa 780
atgaaggttt taccatacac aggtgaagaa atgaatatct ttttatctgc cgtaggcggt 840
atcttgtctg tagtatctgg gtttgtcatc tttaaaaaac gcaaagctaa agta 894
Seq ID 93
atgaagcaac aaacagaagt aaagaaacgt tttaaaatgt ataaggcaaa gaagcattgg 60
gtggtagccc ctattctttt tataggtgtg ttaggagttg taggattagc tactgatgat 120
gtacaagctg cggaattaga tacgcaacca ggaacaacga cggtgcaacc cgataacccc 180
gatccgcagg taggtagtac aacacctaag acagcagtaa ctgaagaagc aacagtacaa 240
aaagacacta cttctcaacc gaccaaagta gaagaagtag cgtctgaaaa aaatggagct 300
gaacagagtt cagctactcc aaatgatacc acaaacgcgc aacaaccaac agtaggagca 360
gaaaaatcag cacaagaaca accagtagta agccctgaaa caaccaatga acctctaggg 420
cagccaacag aagttgcacc agctgaaaat gaagcgaata aatcaacgtc cattcctaaa 480
gaatttgaaa caccagacgt tgacaaagca gttgatgaag caaaaaaaga tccaaacatt 540
accgttgttg aaaaaccagc agaagactta ggcaacgttt cttctaaaga tttagctgca 600
aaagaaaaag aagtagacca actacaaaaa gaacaagcga aaaagattgc gcaacaagca 660
gctgaattaa aagccaaaaa tgaaaaaatt gccaaagaaa atgcagaaat tgcggcaaaa 720
aacaaagcgg aaaaagaacg ctacgagaaa gaagtcgcgg aatacaacaa acataaaaat 780
gaaaatggct atgtagcaaa accagtaaat aaaacgctaa ttttcgatcg tgaagcaaca 840
aaaaattcca aagttgtttc tgtaaaagct gcagaatata tagacgctaa aaaactaact 900
gataaacata aagataaaaa attacttatc agtatgctta gtgtagattc aagcgggtta 960
acaactaaag actcgaaaaa agcacatttt tattataata acggtgcagg aggaacattg 1020
gttgttcttc acaaaaatca accagtaact attacctatg gcaatttgaa tgctagttat 1080
ttgggtaaaa aaattgctag tgctgaattc caatatacag tgaaggccac acctgattca 1140
aaaggtcgat tgaatgcttt cttacatgat gatccagtgg ccacaattgt ctatggaatt 1200
aacattgacc ctcgtacaaa gaaggctggt gctgagattg aaatgctcgt tcgcttcttt 1260
ggagaagatg gcaaagaaat cttgccaacg aaagagaatc catttgtatt ttcaggtgct 1320
tcattaaatt cacgtggtga aaacattacg tatgagttcg taaaagtagg aaacacggat 1380
actgttcatg aaattaatgg atcaaaagta gctcgtcatg gaaataaagt ttattctaaa 1440
acggatattg atgtagggac gaatgggatt tcaataagtg actgggaagc agttcaaggc 1500
aaagaatata ttggcgcaac tgttatttca acaccaaata gaattaaatt cactttcggg 1560
aatgaaattg ttaacaatcc agggtatgac ggaaattcga tgtggttcgc atttaatacg 1620
gatttaaaag caaaatcaat tacgccttat caagaaaaag gacgtccaaa gcaaccagaa 1680
aaagcaacga ttgaattcaa tcgatacaaa gccaatgtgg ttcctgttct tgttccgaat 1740
aaagaagtca ctgatggcca gaaaaatatc aatgatttaa atgtgaaacg tggcgattct 1800
ttacaataca ttgtgacagg ggatacgaca gaacttgcca aagtagatcc aaaaacagta 1860
acaaaacaag ggattcgaga tacctttgat gcagaaaaag tgacgattga tttatccaaa 1920
gtgaaagttt atcaagcaga cgcaagtcta aacgaaaaag acttaaaagc tgttgctgca 1980
gcaattaatt caggaaaagc taaagacgtg accgcttctt atgaccttca tttagaccaa 2040
aacaccgtta cagcaatgat gaaaaccaac gcagacgact ctgttgtttt agcaatgggg 2100
tataaatatt tacttgtctt gccatttgta gtgaaaaatg tagaaggcga ttttgaaaat 2160
acagctgttc aattaacaaa cgatggggaa acggtaacaa atacagtgat taaccatgtg 2220
ccaggtagta atccttccaa agatgtaaaa gcagataaaa acggtacagt tggcagtgtt 2280
tctctacatg ataaagatat tccgttacaa acaaaaattt attatgaagt gaaatcttcc 2340
gaacgtccag ccaactatgg cggaatcaca gaagaatggg gcatgaatga tgtcttggac 2400
acgacccatg atcgtttcac aggaaaatgg cacgctatta cgaactatga ccttaaagta 2460
ggggataaaa cgttaaaagc aggaacagat atttctgcct acattctttt agaaaacaaa 2520
gacaataaag acttgacgtt tacaatgaat caagcattat tggcagcgtt aaatgaagga 2580
agcaataaag taggcaaaca agcttggtct gtgtatctgg aagtcgaacg gatcaaaaca 2640
ggtgacgtag aaaacacgca aacagaaaac tacaacaaag agcttgttcg ttctaatacg 2700
gtggtgacac atacgcctga tgatccaaaa ccaaccaaag ccgttcataa caaaaaaggg 2760
gaagacatta atcatggaaa agtggctcgt ggtgatgttc tttcttatga aatgacttgg 2820
gacttaaaag ggtacgataa ggactttgcc tttgatacag tcgatcttgc gacaggcgtt 2880
tctttcttcg atgattacga tgaaacgaag gtgacaccaa tcaaagactt acttcgtgtc 2940
aaagattcta aaggggaaga cattacgaac cagttcacga tctcttggga tgatgccaaa 3000
ggcacggtga cgatttctgc caaagaccca caagccttta ttttggcgca tggtgggcaa 3060
gaattacgtg taactttacc aacaaaagtt aaagccaatg tttctggtga tgtgtataat 3120
ttagcggaac aaaatacatt tggtcaacga attaaaacca ataccgttgt caaccatatt 3180
ccaaaagtga accctaaaaa agacgtggtt attaaagtcg gtgataaaca aagtcaaaat 3240
ggtgccacaa tcaaattagg ggagaaattc ttctatgaat ttacaagtag tgacattcct 3300
gcagaatacg ctggtattgt ggaagaatgg tcgattagcg ataaactaga cgtcaaacat 3360
gacaaattta gtggccaatg gtctgtgttt gccaattcta cgtttgtctt agcagacgga 3420
accaaagtga ataaagggga cgacatttcg aaactattca cgatgacctt tgaacaaggg 3480
gtagtgaaaa tcacagccag tcaagccttt ttagatgcga tgaatctaaa agaaaacaaa 3540
aacgttgcac actcatggaa agcgttcatt ggtgtagaac gaattgcggc aggagacgtt 3600
tacaacacaa tcgaagaatc tttcaacaat gagaagatta aaactaatac ggtagtgacg 3660
catacgccag aaaaaccaca aacgccacca gaaaaaacag tgattgtacc accaacacca 3720
aaaacaccgc aagcaccagt agagccatta gtggtagaaa aggcaagtgt ggtgccagaa 3780
ttgccgcaaa caggcgaaaa acaaaatgtc ttattaacgg tagctggtag tttagccgca 3840
atgcttggct tagcaggctt aggctttaaa cgtagaaaag aaacaaaa 3888
Seq ID 94
atgaaatatg aacgtccatt aaaaagagag tcacaaatca aggagtttga gttaggcacg 60
cacgcggcag taattgaaaa agttcaaaag aaacggtctc aaaaagggaa tgatatgttt 120
ttactctctc ttttgggaaa aagtaacgaa aaaggggttt attttcttac cttcgggaat 180
gattatactg aagataattt acgctatatt ttagccagta tccaagacaa cggtgtagag 240
attccagacg ttgactttgg ctataatcga gaaacttttg aatttctgaa aggtaaagat 300
gtctatattc aggttgaaga acaagagtat aaagggaaag ttaaacatgc ggtaacaaat 360
tttttaactc aggatgaatt tgaagaaagc gaagaaatgg agttttcaga aagcaatact 420
gaagaagatt gg 432
Seq ID 95
ttggtggaac aaagagaaat tgtacaccgc tattatgttt taggtactag tactttagat 60
aaagtattaa aagtgctgct taatttatcc agtgatggtg tattggcgct aaaagacaag 120
tttcttcctt taaaagggga aaataatttg tataagctaa tgaatcgtga ggatccactg 180
acttcggcgc aacttcatga aaaagtgaat ttaaataagt taaaagaaca gttggaagcc 240
caagggcttc cttttgcgtt taaagaaacc aaagaaggga ctaacttcta ttttcgagta 300
aaagatacgg aactcgcaaa gaaagcctta gaacgcgtat taacggatat taagaagaac 360
ccacaaatga ttttgcgtaa gccaaatacc atgacttttg atgaacggtt agcctacact 420
gcagccaata aaaaatacgt gggtaaaatc gatcaaacaa aagcaatcac aaaaggaagg 480
aaactg 486
Seq ID 96
gtgcgtatga ataaaaaaac accaaaacca ttaaccccac ccaaagcctt ttttaactgg 60
tgtactgcac aaatacccac gtatgaatgg caaaacaaaa aggagacaat tcttgcgagt 120
tctcgcaaga attgccctat cataaaaaaa cgattaacaa aatactcacg attatcgttt 80
ccaacgaaat tttactcctt tggaatcatt ctcgttcgtg caaaacgaat tgaaattcaa 240
acccatagtt attggcagac gattacagat ggaatagaaa accttatcta tgagccgtca 300
aaccttgaac ggttttcaaa cgatacccat gttaaagccc attatgagaa cggacattgg 360
catgagggct tgttagcaaa ttatggtttt atgagtagtg cttatacaaa tacagtattc 420
tatccaaata actggcaaga aaaactaacc actgtatcgg aattaaagta cttacagtta 480
cctgaaatag aacgtcaaga actagctcat atttacaaat accgaaacga aattgagttt 540
ttacaaaaga taggtgccac aaccttagcg aatgaaatca tttttgacga ctatcgcaac 600
gtttttggac tgtattttca taaagtagat atgagagtaa ttactaagaa atggctaaaa 660
gcgaataaac aaaaattaaa aacaagaaat ccaacctttc atgaattcat gttagaaaaa 720
acgttaaaag agcgaaatgc gcctatgata aacgaaatag aaaagtacgt gcattattcg 780
caaattaagc aactaccgaa agaagtgaac ttaacaaagt ttcaaaaatg gtttatacga 840
aaaggggagc gttttgacta ttatatggac tatttacaca tgttagaaga gttgaacacc 900
ccacttaata acgatagtgt attgtatcct gaaaacttac aagtagctca cgataacgct 960
atgaacacgc taaacctttt gaaaagtgag attgaagaaa agcagtatca agaacggaaa 1020
aaccaaatta aagcgttaga agctgaaatt gacgatttgc tttttctcac accacattca 1080
ttacaagaaa tcattcaaga aggaagtatt ctacgccatt gtgtgggcag tcaacactat 1140
atcgaacggc atacgcaagg aaaaacaacg attgttttta tccgaagaaa agaaaagcca 1200
gatatgcctt acttcacatt agaatatcga aatcaacagg ttatacaaat acaaggaaaa 1260
tgtaaccgtc aggaagtacc agaaaagata aaacaagcag tcagacaatg gcaagagaat 1320
ttacagcacg ccttatcatc a 1341
Seq ID 97
ccaagtgaaa agggaaacca aggaagggtt gaaaagagaa gcgacgcaaa aagtacgaac 60
cacgttaacg caggaagata cactgaa 87
Seq ID 98
gagaacgaat tcaagctgca caaaattggt ttacattctt ttctcgatcg ggtacaccaa 60
tcggtggttc tgggagtgag gtctttgcgc agtataaaga aaaaatgcaa ccattaccca 120
cagacagaga aacaaaagca ggacaaggct ggccaggcaa tgcgtatgcg ccagggaatt 180
gtacttggta tgtgtttaat cgttttgcac agt 213
Seq ID 99
aacatttttt ccaaaagcag tgctcctatt attcggcgct ttattaggtc aatggctagg 60
tgggatgttt gctttcttga tgataggtcg cttgatgcag gtgccagttt cgatggtttc 120
tgtttaccca atgtttgtta tcgctacgtt gattgggatg ttaactatgg ttcctggtgg 180
tatgggaacg tt 192
Seq ID 100
aggggaagat gttacatatt taacgaagtt cccaggtgtt ggcaagaaaa cagcccaaca 60
aatgatttta gatttaaaag gaaaattagg cgaattagag tcttccgaag cagcggttgc 120
ggccatgacc gcaaccgaag cggtgacaac aagcaatcaa gcattggcag aagcgttaga 180
agcattaagt gccctaggct acag 204
Seq ID 101
accagattgt ggggaagaca gctatgtcgg aaatcacaaa ttagaaaatc gtcgggtatt 60
aattacaggc gg 72
Seq ID 102
cttggtttgt catttataaa acacgttttg gcttacggtt acgttctgtg ggggaacatc 60
cacaagcggc agatacattg ggaattaatg tctact 96
Seq ID 103
cacttggttt tcgtccatgt aaaagatgtc aaccaaccgg gcaagtcgtt cctaacgagg 60
aatgggttgc tcaaattaag caatttattg atcggcattc agcggaacca t 111
Seq ID 104
aagacggtag tggcgcggca gctgcgatgg aaatggcgat tgcagaagca gggattacac 60
cggaacaaat tgggtatatc aatgctcatg gtacgtcaac cccagcgaat gacgctgcgg 120
aaacaacggc catcaaacgt gtctttggtg agcgagcatc gcaaattcca atttcaagta 180
caaagagta 189
Seq ID 105
tgctacagca aatttaggca aacatcaaaa caatggccaa acaagaggcg acaaggcgac 60
Seq ID 106
gtggctagtc cctcgaaaag tgcgccattt tttacgcacg cgaaaaaatg tcaaaatcaa 60
cggagaaaca gctgcttttc attttccagt ccaagctggc gattgtgtca ccttaacgtt 120
Seq ID 107
aacaaggggc ggcagccaat ttacagaaag ctccagcgcg cacctttgat cctaaagtat 60
ttaagcccag cgcaagtaaa ccagcttata cacaaccagc tacaaaatca gttagcaaca 120
aagtggcaag tggtggcgaa aaagtttctt ggcaagcagg tgataaggtc caacataaag 180
catggggcgt tggtacagtc gttcgtg 207
Seq ID 108
gagagcatca aggatttcaa caacgtttcc agaaaaaaac agataaaaag agtccaatca 60
aaatggaaga ttatctttta ctcagtcgtc atttcactgg actcagccgt gaaacctatc 120
gggaagttct ccgatatttt caaagaggcg cttttcagca atactactcc gaagaccaag 180
aagtcatcag tcaaagaata cgtgaaaatt 210
Seq ID 109
cacctgtggt tggtgctgcg gtacctgccc aagcagaatc aagccagcaa gcatttatta 60
atgaaatcgg aaattcagca gcagccgttg ccaatagcaa tgatatgtat gcttccgtca 120
Seq ID 110
gtcatgtttc ggcaaaatca caggcgcgac aagaagaaaa agaggcgaag cgagcagcga 60
agaaagaagc caaagctgca gaacgccaag caaaaattga agccgctgcc caacaaaaat 120
tacaagagcg ggaacgcatg gaacaagcgg cagccgagcg ct 162
Seq ID 111
aggattgcga attggaattt ggaatatacg aataatttgg aggtaatact tttgggtaaa 60
aaaggttcaa agattaaaaa gaaaaaacgc cgacttaaag aaaaagctat tgcaaatggc 120
acatacagca agcgagggaa aagcgatggt atgtataaaa tgcaagggac agatgattgt 180
ttgggaaaaa ga 192
Seq ID 112
aaaacgaata cggcattggc gggcatggct acattggcta tgatgaatgg cacgacggca 60
aaggcatcaa gctgtcccgc gccgacgatt tttccggtgg caattatgat acggttacgc 120
Seq ID 113
cgattgcccg cgccgccgct gaggaaaacc cgcagtttgc catcaatgcc gaaaagctgg 60
aacaggtgca gcccaaagac c 81
Seq ID 114
ggaggtggaa cccatttgaa acaatatgaa atcattctgg ccgaccctcc gtggagatac 60
tcacaaaagg ggggtgcagg gagcggcaga acgtcactac gccaccatga gcatggagga 120
tttatgctca ctgcctatca gccgttttgc tgcgccggac tgcgcgcttt ttctttggag 180
tacattccca cagcttccag aagcactgca tctgattcgt gcatggggat tcaccta 237
Seq ID 115
cgaaaacaat tgatcacagt cgtttacaag tcaaagattc aacgatttat gtcggcgatt 60
catggaaacc agaagagaac tttgtttcag caacagataa aacaggtcaa gacgttccct 120
tcgaaaaaat cactgtttca ggtcaagttg ataacaccaa agcaggcgtt tatccaatta 180
tttacagtga cgaaggtaaa gaagaaacag cctatg 216
Seq ID 116
caggaattac ggatagtggt tatacagata caaataaacc agaaacggat acgccagcaa 60
cagatgcagg cgaagaaatt gaaaaaatac ctaattctga tgttaaagtt ggcgataccg 120
tcaaag 126
Seq ID 117
ttatcatggc aaagcagtgc aagcagaaga tgttttggaa atcgtgacaa atcaataccg 60
agcagtcggt ggcggaaact atcaaatgtt ccaacc 96
Seq ID 118
agaaacacgc aagggaaatg ccgattttcc ttgcgtgttt cgctgttttt cggtaaagta 60
tgtagaaaag tagggagttt tgctcatgaa ttatccattg atttttgcag aaaaccaacg 120
aatcgaaacc gaacgcttga ttttaaggcc cgtcacatta gccgatgcag aggatatgta 180
cgaatatgct tcaga 195
Seq ID 119
tttcccagcg gacttctttt cgccagagga aacgttggcg ctggttccgc aagcggtgat 60
tcaagcattc gcggaacagt tgaacacgcc acttttaacg gcagccgaac aagcggcggc 120
accgaatctg ctagaagcat t 141
Seq ID 120
aaccaacgga agaaattcaa ccaaaaccag aatcaatcac ccctcaggaa aagtcagaac 60
cggaacaacc tgcgccaacc cctccggaag atcaaaaaac tacggttcct tctgaagaaa 120
aaaagccttc acccaagaaa aaagacaaac ctgtggagaa accggcaatg tcaaaagaaa 180
aaaagaaaca aacaaaagaa acaacggctc atcccttaca ag 222
Seq ID 121
caacaagttt gtttggcgcc tttgtcgcac tccttgcgag catgggaatc atcaaacgca 60
aacgtaaaaa ctagctctct cctt 84
Seq ID 122
gctgttatgc acgaccaaaa gacaaaccga cagcccttga cccaatcaac gaagaagaag 60
ccaaagaaca agcaaaatat gcggtcaatg gtttccctca agattttaca gaatggtacg 120
agtgggaaat taacaatgga aaacttaaaa atttactata aaaaaagaaa ccttttgcta 180
acgacaaaag atttc 195
Seq ID 123
ccaaactctt gtgtatacca catgaatgac caacgaatta agttccaagc aacgaagaat 60
aagattgggc ctaagatgtt accacccatt gctaaggaag cacctaaagc gcctaacatt 120
ggacgaacag taaaccagaa aactgggtca ccgacaccgg ctaaaggacc catcatccca 180
actttaaccc ct 192
Seq ID 124
attaaatgtc cgaccagccg tcagctgaag catttctttt ttcatgcctt ccgccaaacg 60
tgcatcccca aagctttcaa tcatatcaat aacggcgatt acttctttat aatatctcat 120
tttgccagtg tacagataat acgtaaggtt ttt 153
Seq ID 125
cgccatatca gtaatggggg caccttcctt gcggttactg aaatggccat tgatgacttt 60
gatagccgaa ctttccaaat tttc 84
Seq ID 126
tccacacttt caataatcgc tttaatattt tctaaacatt gcgtcgtttg ttccttgatt 60
ccgccagcga ctaacgcatt tgttttaggg tccaatggta gc 102
Seq ID 127
aacaagtcca gtgctttttc accagctaag cgatgtcccc ctttaaccac gacatgcttg 60
gctcccattt gtacaattcg ttttgccgct tcttccatct ccgccacgga agaaatttcg 120
cctaaaccag ataagatgcc cgcttcaatt aaattaggcg tggccactaa tgctaatggc 180
agtaaatcgt tttttaggcc ttccacactt ttgggttgca gaatttgtgc cgttccctta 240
caagcaatga ctgggtcaat cacgactttt 270
Seq ID 128
ttgccgttgg ttgtttataa tgaatcaaat cgcaaaatgg atgcacttga aaactcgtgc 60
cgacaatcac gattaagtcg gcagaagcaa cggcctgaat ggctttttca attgcctctt 120
cggatagtcc ttcttcatac aaag 144
Seq ID 129
accagtaatg acagcatcat gattgctaaa ctaagcaaca gttttttcaa aaagtttcgt 60
tctcgaacaa aacaattaat cgctgcatcc gattgtcgga ctaaaacgaa taacgtcgct 120
agtaacccaa gaatagtaga ccaactagca cctaatgttc cccaacgaat ggtgaaaaag 180
cccgttgtca gtaacttcac taataagccc acaccagctg ccactaaagg acctttaaaa 240
cgattgcgac tttgttcaat actttgg 267
Seq ID 130
tcagctttaa tgtctactac acggccttct ggtttatcta ataaggtaac aattttcaca 60
gatgcagctt tacggtatct aaacaaatct actaaatacg ctaatgtacg accactatcg 120
atgatatctt ctacgattaa aatatgtcta ccttcgacat ttgtgtccaa gtctttgaca 180
atttttactt cgcctgaaga aactgtcgcg ttgccg 216
Seq ID 131
cacgtgttac agaaatacca acgcccgcaa tacctacgct atccgcggaa aattgagcca 60
tttccccagt ttgtaatttg ctttctggaa tttcaaccaa tacaaaacct t 111
Seq ID 132
atgtcaataa tcacaccagc agataccttt ttgaatcgtt taatcaacgt agctgctgac 60
tctttcaagc catttctttc taacgttcct gttgctaaca taataataat aaagatagcc 120
atgcctcgat tattcacaaa ggttgttcct agtgtttcta gcattgatgt caaaccaagc 180
ccgccagtaa aagccgtcac tattaaagca accatcacaa tcaagatagc atcaaatttc 240
aatgcgaagc cgacaatgac gatcaatacg cctaataaag gtaataaatc catcaaatct 300
ctccttctta aaataaaatt tcactttcca ttatatgcca attttcgctc atttgtaaga 360
cttttt 366
Seq ID 133
cggctaaata gatcggttct ttcgttggtt cctctgcttg cagtattagg cgataaacaa 60
aaaaatcacc ttgcgttttt tgtaaaagct gctgctgtaa gtagcttaat agttcaccat 120
Seq ID 134
agtgtgtgta ttgctcgcca cctgccacat ttggatgaac tcggtcatca taaaaccaat 60
catcatgggc gttactataa t 81
Seq ID 135
gttgcaccaa acaaggtatc aggacgggtt gtaaacaccg tgaaactttc ttctgtgcca 60
gcgactttaa aggtcacatt ggctccttca gaacgtccaa tccaatttcg ttgcatatct 120
ttaatactct ctggccaatc aacaagctct aaatcttcta ataagcgatc tgca 174
Seq ID 136
atgattcctg catccataaa aatgacacgg tccgcgactt cttttgcgaa gcccatttcg 60
tgggtaacca ctaccattgt catcccttca atagctaaat ttttcataac ggatagcact 120
tcgcccacca tttcagggtc taaggcagaa gttggttcat cgaacagcat cacgtcaggg 180
cgcatggcta aagcacgagc aatcgctacc cgttgttgtt gcccaccaga aagt 234
Seq ID 137
cataatccca tgaaaattga agcaaaaaat gctggaatcg catcttctaa atccgtccaa 60
ttgatgtctt tgaaagaagc taacatcata attcccacta aaattaacgc tggagccgta 120
gcttgtgctg gcacaatagc aataagtggt gaaaaaagac tacttagggc aaatagaatt 180
gctaccacta ccgatgtcaa acctgttcga ccacccgcac cgattcctgc tgcactttct 240
aca 243
Seq ID 138
tattttttcg taatttctac gaagtattca tctaacaaca agttttcaag tgctggattt 60
ttttcatatg catcggtaat tttttgtaag aattgtgcac ggataataca acctgctcgc 120
cagatttttg caatttcacc aaatggtaaa tcccatccgt attcttcaga agctgcacgg 180
agttgtgcaa aaccttgcgc a 201
Seq ID 139
ccatcttcat caatcaatgt gacttttgtc gttgtggagc ctgcatcaat ccctaaaaag 60
gtcacgccag tatgt 75
Seq ID 140
gcaataataa acgatgtccg tcctttcaga agttgttgta gtccttcttg taacaattct 60
tctgtttttg tatcaatact ggaggttgct tcgtctaaaa ttaaaatttt gggatctgcc 120
agtaaagcac gagcaaatga aattaattgg cgttgtcctg ccgagagtgt actaccccgc 180
tcttccacaa ctgtttca 198
Seq ID 141
tcttcctctt ctggaaacac ttgtggccaa aaagattcag ctagccaaaa taattgctct 60
gttacagttc ctttaaactt aggaagttcg tcttcagcca agggcaaact cgcaacttca 120
gctgccgta 129
Seq ID 142
atcatccccg cttcaacagg tgaatgtaaa gcatcgatgc caaacatagt taaggccaat 60
gcaatcgcca acgtatacaa caaattaatg atgggactta ataataacga attcatacga 120
atcattgcta ctcgtgtttt taaa 144
Seq ID 143
actaattgca ttgctaccgcc acaagcgatt cctaatttta aatctcctaa aataatgcca 60
caaactaatc cgccgactaa aggacgccca atcgta 96
Seq ID 144
tacccattcc aaatagacaa atcaacgcct tggtctcctt ttgcagcaaa tgtgctaatt 60
ggtgatacaa aaaataagac taccagtaac gttgccaata gtttcttttt c 111
Seq ID 145
ttttttaatg atttaagaat atccataatc actttttcac tagacatatc aatgccgacg 60
aaaggttcat ctaaaaaaat cacctcagca ccttgggcca aaacacgggc gataaacaca 120
cgttgtaatt ggccaccaga aagttcgcca atctggcgtt gcgca 165
Seq ID 146
tttttacaca agagtcattg tcctgtagaa ccgaaaaaaa cagttcctgt gccttcgtta 60
atttgt 66
Seq ID 147
gacacaaatt gctgtgcaaa cagtgttgcc gttacaccga caagcagtaa caggacaatg 60
atcgta 66
Seq ID 148
gggtcgccgt catcgtcata ccgggtttcc acgatcacct cacgggtgag ggtgtactgc 60
cgggaaaaaa tgcggtcaat ttcgggctgg acttctgcaa aggtaaaagc attgaacgcc 120
gtggagagg 129
Seq ID 149
ttgctgtgcc actattgttt cttcctgatt ccacagtgtg atagaaacaa cagggacagt 60
ttgtgccacg tccttggttc c 81
Seq ID 150
aagacaatcg caaaggttaa taatgcaact aaccaattcc gggctgaacc aaaatcagca 60
ctaccagctc ctcctgccat atacttaatc gcaacaggaa acaaggatag tccaatgctt 120
aaaataaccg ttcctgttac taaaggcgga aacaacacac ggattttttt aatgaacaaa 180
ccaacaataa taacaagaat actgccaact aattgggaac caaaaattgc tgcaatccca 240
aaatcagctc cgatggccat caaaatcggt acatacgcaa aacttgcccc catcataact 300
ggtaaccgtg aacccacttt tccaaaaata gggaaaagt 339
Seq ID 151
ccaacactca tattaaaaat gaaaccattc gggtcaccta gctcgaactc tttactaagt 60
aacttcaaaa cgaaccatgc tttgacgtat tcatca 96
Seq ID 152
ggaacactat caatcacagt tgcaccagta tgcaccatga ctgctgctga aagtgaagat 60
gtgcccatat ctaaaatcgc tggtccaaaa gaacctgtcg ctaaaataac cgctgtggat 120
gttgaaccaa ctgctaagcc cattaaaata ccagaaatcg gtgccaagaa ggttccagaa 180
attcccataa cagaaattaa gtggacaatt tgtgttgata aatcagaaac agaaattaat 240
ccagcaattg cacctgcacc aattaaaatt aaaacggtat cggttactct cttcaagcca 300
gaagatgca 309
Seq ID 153
actaaatcac gtgctaacgt ggatacatta caagaaatat acaccatttt cttaggtggt 60
tgtttcaaaa gggccgttaa taatttacga tccaaaccag tccgtggggg atcaactaca 120
atggcatcag gtttaaagcc ttctttgaac catttaggta ataagtcttc agctgttcca 180
acagca 186
Seq ID 154
ctttcctgtg aaactaaaaa gtccacgggg ccatcactgt tgaccgtgtg catactaatc 60
gtatacacaa aaaccatcac gccgatccca ctcaaggctg tcgcaattaa taaggtcaaa 120
ccagtgagtg ttttgctact tttaaataag 150
Seq ID 155
tttctctttt cacttccggt aaggcgtgaa ccaccactaa aattccataa acaaagttcg 60
gtgctagccc cttttgttcc aataacaaca ataattcttc aaaatcaaca cctacagcaa 120
aagctagtcc taaaccggca aaggcatacg ttcggc 156
Seq ID 156
aataaaccag caaaaaacgc gccaaaaacg gcacctgtta acgctaatgg cggaatacct 60
aagaatgtca tacggattaa gccgcagaca agtgccatga gcgttccg 108
Seq ID 157
ataaattccc aaatagcgcc aatgcccata aattgcctca acctgtgcgc cgatataata 60
aagagtaacc atattcaaaa taatatgcat aaatccaatg tgaagaaaca ttggcgtgat 120
aaaccgccag tattcatgat tttgagctac cagtggacga accattgctc cccaattaac 180
Seq ID 158
ttttcgtttt cgctttacga attcccgcat aaaaagcggc aaataatcca acaggaccgc 60
cgccga 66
Seq ID 159
gcaaagaatg cattaattcc taaagctggt gctgtcgcaa tcggatattt cgccaacaca 60
cccattaaaa tacaacctaa ggcactcgct aaagcggtgg cagtaaaaac agcgccttcg 120
tccatgccag aagcaccaag tacggttgga ttaacgaaca aaatatacgc cattgagata 180
aacgtggtga aaccagctaa catttctcgt ttcatattcg tattcaattg ttcaagttca 240
aaatatgaac ttattttttc tttcatgttc cgtcctccta aaatc 285
Seq ID 160
cttttttcag atacattctc tatttccctc accgcattcg tttctttaaa ttcaatcatt 60
ggaaatagcc gacgcaattc cgattgcaaa atcaacgaag aactgatacc actgggacaa 120
acaattagcg cttttagctt ttcttgccgc tgttcatttt tttgcccgaa aatttctcca 180
ccaaataata tggtaaaaaa accgatttcc tctgacggga ttgaccgttt cgttaatttt 240
tctaaaggag ctaacgcttg ttcacaaaga 270
Seq ID 161
ataaattccc atagcataca acgtgttatt cgggtcccat gtccaaaaag ctcgtctggt 60
tctaaaaaaa gttccagcgt cagt 84
Seq ID 162
gtaaatcaat gtctgaaacg acaaatttat cgccatattt ttttgataca tcactcatct 60
ggatcatgtg cgttgcttcc tttctgcaat aattttccat ataaaataaa cgcccccaac 120
aaattgaatc accacactga gcggggtatt caaatgaaag acctgttcaa ccatcaattg 180
accaaacacc ag 192
Seq ID 163
tcggccatct caattttttc tgttccagag ctgatgtata aagtaacaga tcggcctttt 60
ttcacagatg attttgcttc ggggtctgtt ttgaccacct tgccttcttc aatcttatcg 120
tcgggaattt ttttggtttc actatcgact ttcagcccgg cactttttag cgcttgtgaa 180
gcgtccgctt tcgtttcatt tgtaacatca ggaacttcaa cgtctttacc gcccgacatt 240
gcaaaggcta agccacctat tgctaaagaa attaatgccg caattgctaa gccaatccac 300
aattttttac ggtttttctt cggcggttgt tcagctaaaa tgtcatctgc gatatcttct 360
gggacttcga caggcgttgt ctcttccggt tcagcgatgt cttcgggaat cggagttaat 420
actttcgttt ctcctaataa agccgttggt tcccacgcag gttcgtttaa acgactggct 480
gacaaggacg tgtataagtc ttctgccatc tcattcgctg ttttg 525
Seq ID 164
ggatcaccgc cacctcctgt ccctaaaaaa gctgctcctg tggctatatt ctcaattgct 60
tctgcatcta aa 72
Seq ID 165
tctccagcaa ttactgcgcc taatgcaaaa gaaccgacag tcattgaaag acctgacata 60
aagcttcctg ctgtt 75
Seq ID 166
attcgttccc gtaatgatcg cttagctagc gggacactga tttccttctc tgtaaaatgc 60
aacactagtg caaacaaaat ggaaaagcca atgcctaata acatacagcc actcatgaac 120
gaaatttttg ctagcggaat gctcgtactg gcacccatcg ctgtcaccat cacaccaaag 180
aaggcccctg gttttgaaat atcg 204
Seq ID 167
atgtttgtaa acattggcat gaacccaatt ttcattgaca gaaaggcaat caagaccgat 60
acaatcggcg cgaccgcaat cggaatcaat aataaagggt taaaaataac tgggagtcca 120
aaaatcaagg gttcattaat gttgaaaaat gctggaacaa acgaaatttt cgacatggct 180
ttcattctag atgaatgtcc gacgatggcc attgcaatag ccaagccaat cgtgcaacca 240
gcgcctccaa ttgtagcaaa t 261
Seq ID 168
caagccaact cggttcgtcc catgaattcc catgcgcacc tacaggcgga ttcaaataat 60
ccccttctgc acgttttgga t 81
Seq ID 169
gtaaaattac cagtaccatc attcgaaata tcttgtgctt tttgttcaag acttgcgcat 60
cctgataata gcaaagtggc tgctgttagc agtagccact tttttccaat aaatagtttc 120
Seq ID 170
gtgatccaac aagaaagtcg tttaagagtc gctgacaatt ctggcgcacg tgaaatttta 60
acgatcaaag tcctaggcgg atctggtcgt aaaactgcta acatcggtga cgttattgtt 120
gctactgtca aacaagcaac gccaggtggg gttgttaaaa aaggtgaagt agttaaagcc 180
gttatcgttc gtacaaaatc tggcgctcgt cgtgctgacg gttcttacat caaatttgat 240
gaaaatgctg cggtaattat ccgtgacgat aaaagcccac gcggaacacg tatcttcgga 300
ccagttgcac gtgaattacg tgaaaacaac ttcatgaaga tcgtttctct agcaccagaa 360
gtatta 366
Seq ID 171
MVGIILASHGEFAEGILQSGAMIFGEQENVKAVTLMPSEGPDDVKAKMQEAIASFDNQDEVLFLVDLWGGTPFNQANSLLE
DHKDKWAIVAGMNLPMVIEAYASRFSMESAQEIATHILETAKDGVKVKPEELQPABAPKAAATEDAQPKGSLPPGTVVGDG
KIKFVLARIDSRLLHGQVATAWTKATQRNRIIVVSDAVAKDDLRKKLIEQAAPPGVKANVIPISKMIEVAKDPRFGNTKAL
LLFENPEDVLKVVEGGVEIPEVNVGSMAHSVGKVVVSKVLSMGQEDVDTFDELKAKGIKFDVRKVPNDSKANMDEILKKAK
NELANA
Seq ID 172
MKNKSRQFLKNNWPYMLASFFIPFLIMAIIXLSIGIYPGSSRSVLASDAFSQFSNFHASFNNVLHGKQSLFYTWNASLGLN
YLSLISYYLGGLFTPLVFFFNNQNMPDALYFLTLLKIGSAGLSFWFLAKQTFKIPKWSHVTLSVSYALMSFIVAHSELIMW
LDAFIYLPLVILGIHRLMDQRKPTLLFVSYFLLFITNYYFGFMIGLFSFLYYFARTFTDWQRYKSRIVAYFTTSLLAGGAS
MIMVLPAVLDLRTNGETLSEITTFKTEATAFLDIIMRNMIGVYDTTKYGSIPFIYIGLLPLIFCLFYFVTKEVPLKNKLLF
GSLFVLLIASFYITPLNLFWHGMHAPNMFLFRYSFLFSFLVILLAGYGWEKFEKDDLGVLSGLILILLAIFALAMGTKGAT
SYTYVTLTSFVLTATFLLLYFFGIAFYQLKKAPMQYLVILLLLLVSGEAIINTRAMVTGILDDWNYASRSLYSEPYPDLKN
LVDQTKKENDTFYRLENLNGVSANDGINYGYSGISMFSSVRNRHSSTYLNALGFRSRGTNLNIRYQNNTLLMDALMGIKYN
IAENNPMKFGFERQAAAGKYQLYRNENALPLGFLADKEIYNVRQPLNDNLGSQTNLLNALANTNERYFTFYQPTMTLQNNV
TITQNTAGVTFTEKQHNVAKEISYTVNVPANTQAYLSLFPTDFAQLESSTATVTVNGSSQQSQIGITGQYYNLGYYPKDTT
VNFKVSFYGTKAVSFVQPQVVGLNTNAFEKAISAVQEKGVDLTTGKRSASGTFTADKDQVLVTTIPYDKGWRVKIDGKKVT
PKAFKDAFLSVPVSAGTHTIQFSYLPKGLIPGIVLFVLCTGGFVAYVTLIPARRNRKKEDK
Seq ID 173
VKKLSFKKVKWGMHFLMAVALIAPSVTSTAYAVETTSQQSSEAVTSTTDSSRKQEPVITQETTDIKQEAPNQATSDSVKQS
QETTAPTETTNLETSIAEKEETSTPQKITILGTSDVHGQLWNWSYEDDKELPVGLSQVSTVVNQVRAQNPAGTVLIDNGDN
IQGTILTDDLYNKAPLVNEKTHPMITAMNVMKYDAMVLGNHEFNFGLPLIKKIQQEATFPILSANTYNKEDGLRFVEGTTT
KELDFNQDGQPDLKVGIIGLTIPHIPLWDGPRVTSLNFLPLKEEAEKAVTELKANDQADIIVASIHAGQQNSDPAASADQV
IENVAGIDAYILGHDHLSFTKQGAAPNGKTVPVGGPKDTGTEVVKIDLSVAKNADKWEVQEGTATIVPTTNVPADEAVKAA
TKEYHEKTRAFIQEEIGTATADFLFKQEIKGIPEAQLQPTAMISLINNVQKEVTGAQLSAAALFKYDSKLPAGKISYATIF
DIYKYPNTLVSVPINGENLLKYLEKQGAYYNQTQPDDLTISFNPNIRVYNYDMISGVDYKIDISKPVGERIVDAKIDGQPL
DPAKEYTIAMNNYRYGGLASQGIQVGEPIKNSDPETLRGMIVDYIKKKGTLDPEQEIERNWSIIGTNFDEKWRAKAIELVN
DGTLQIPTSPDGRTPNAAAITKQDVRNAGFDLDNAYTIMHTNDVHGRLEAGKGELGMARLKTFKDQENPTLMVDAGDVFQG
LPISNFSKGADMAKAMNEVGYDAMAVGNHEFDFGLEIALGYKDQLNFPILSSNTYYKDGSGRVFDPYTIVEKSGKKFAIVG
VTTRETATKTHPKNVEKVTFKDPIPEVEAVIKEIKEKYADIQAFVVTGHLGVDETTPHIWRGDTLAETLSQTYPELDITVI
DGHSHTAVESGKRYGKVIYAQTGNYLNNVGIVTAPESEPTKKTTKLISAAELLELPENPAVKAIVDEARTNFNAENEEVIV
DYIPFTLDGQRENVRTRETNLGNLIGDAIMSYGQDAFSQPADFAVTNGGGIRADIKQGPIKVGDVIAVLPFGNSIAQIQVT
GAQVKEMFEMSVRSIPQKDENGTILLDDAGQPKLGANGGFLHVSSSIRIHYDSTKPGTRLASDEGNETGQTIVGSRVLGIE
IKNRQTQKFEPLDEKKQYRMATNDFLAAGGDGYDMLGGEREEGISLDSVLIEYLKSATSLRLYRAATTIDLAQYKEPFPGE
RIVSISEEAYKELIGGGETPKPDPKPDPKPTPETPVATNKQNQAGARQSNPSVTEKKKYGGFLPKTGTETETLALYGLLFV
GLSSSGWYIYKRRNKAS
Seq ID 174
MNQQTEVKKRFKMYKAKKHWIVAPILFLGVLGAVGLATDNVQAAELDTQPGTTTVQPDNPNPQSRNETLKTAVSEEAALQK
DTTSQPTTAEEVVPKGIAAEQSSATSNDTTNVQQPTAEAEKSAQEQPVVSPETTIEPLGQPTEVAPAENDANKSTSIPKEF
ETPDVDKAVDEAKKDPNITVVEKPTEDLGNVSSKDLAAKEKEVDQLQKEQAQKIAQQAAELKAKNEKIAKENAEIAAKNKA
EKERYEKEVAEYNKHKNDKGYVNEAISKDLVFDSSIVTKDTKIDKITGGKFIKASDFNKVNQGQSKDIFTKLSKDMNGKAT
GNFQSSKVAAVEFGPKGGYAVLLEKNKPVNVTYTGLNASYLDRKITKAEFIYELQSAPSQSGTLNAVFSNDPIITAFVGTK
NANGKDVKVRLTIKLYDANGKEVLPDKDHAFAYALSSLNSSLGTNYSVEHAEFVSDFGSKNEFKYINGSYVKKQADGKFYS
TEDLDYGTGPSGLKNSDWDAVGHKNAYYGSGVGLAREGGRISFSFGMTTKGKVNLSGAQWFAFSTNLNAKSIRPYQKKGNP
KEPEKATIEFNRYKANVVPVLVPNKEVTDGQKNINDLNVKRGDSLQYIVTGDTTELAKVDPKTVTKQGIRDTFDAEKVTID
LSKVKVYQADASLNEKDLKAVAAAINSGKAIDVTASYVLNLDQNTVTAMMKTNADGSVVLAMGYKYLLVLPFVVKNVEGDF
ENTAVQLTNDGETVTNTVINHVPVSNPSKDVKADKNGTVGSVSLHDKDIPLQTKIYYEVKSSERPANYGGITEEWGMNDVL
DTTHDRFTGKWHAITNYDLKVGDKTLKAGTDISAYILLENKDNKDLTFTMNQALLAALNEGSNKVGKQAWSVYLEVERIKT
GDVENTQTENYNKELVRSNTVVTHTPDDPKPTKAVHNKKGEDINHGKVARGDVLSYEMTWDLKGYDKDFAFDTVDLATGVS
FFDDYDETKVTPIKDLLRVKDSKGADITNQFTISWDDAKGTVTISAKDPQAFILAYGGQELRVTLPTKVKANVSGDVYNSA
EQNTFGQRIKTNTVVNHIPKVNPKKDVVIKVGDKQSQNGATIKLGEKFFYEFTSSDIPAEYAGVVEEWSISDKLDVKHDKF
SGQWSVFANSNFVLADGTKVNKGDDISKLFTMTFEQGVVKITASQAFLDAMNLKENKHVAHSWKAFIGVERIAAGDVYNTI
EESFNNEKIKTNTVVTHTPEKPQTPPEKTVIVPPTPKTPQAPVEPLVVEKASVAPELPHTGEKENTLLSVLGAGMLVGLAW
FGLKKREVK
Seq ID 175
MKRIGYARTTIIEDDLKNQLTTLQSFGCDDIFQETFDPQAEISVLDEVEKLLSAGDTLIVCKLHHLGKTTRQLTDFMKMLK
EKQVDFVSISEGIDTHLPTGEAYFQLMESLSAMECALIKERTLVGLHKARENGKVGGRPKIDGRTVRKIRALYYENKETIQ
FISNKCGVSVGTCYKYINLPETDVERLYS
Seq ID 176
MSKKEINQVVASSYQLYINGEWTTGSGNKMIASYNPSNGEKLAEFVDATNADVDRAVEAAQEAFQTWKDVDVVTRSNLLLK
IADLIEENQEHLAMVETLDNGKPLRETQSIDVPASADHFRYFASVIRGEEGSVKEFDKDTLSIVVKEPIGVVGQIIPWNFP
LLMGAWKLAPALAAGNTVVIHPSSSTSLSLLELFKIFDQVLPKGVVNLITGRGSDSGNYMLAHPGFDKLAFTGSTEVGYTV
AKAAADRLIPATLELGGKSANIIFEDANWERALEGVQLGILFNQGQVCCAGSRVFVQSGIYDQFVEALKEKFEQVNVGFPW
EKDVEMGAQINEHQLEEILKYVEIGVKEGATLITGGQRLTENGLDKGAFLAPTLLANGTNAMCVAQEEIFGPVATVIKFET
EEEVIRLANDSEYGLGGAVFSQDINVALRVARGVRTGRMWVNTYNQLPAGAPFGGYKKSGIGRETHKSMLDAYTQMKNIYI
VTKEEADGLY
Seq ID 177
MGKYHELAEKIVKNVGGQENINSLTHCITRLRFKLKDESQANDDVLKNMDGVVTVMKSGGQYQVVIGNHVPAVYEEVVSIA
GLSGEREEEASSGNLFDRLIDILSGCFQPFLGALAAAGMVKGLNALLVFLKLYTATSGTYTMLNGIGDAIFYFMPVILGYT
AAKKFRLHPMVGIVIGAALCYPTIQGSALQTAFETTAGAGAAAPYNLFGLPAYNTFMGIPWVGANYTSSVVPIIFIIAFAA
QVQKVFKRIIPEVVQTFLVPFFVLLIALPIGFLVIGPIVSMLTDLLSAGFTALMSFSPALYGLILGFFWQVLVIFGLHWSV
VPLAIMQVTQEGSSQVLTGSFAASFAQTAVVLAMFFKLKDKKLKALCPPAIISGIFGVTEPAIYGITLPKKWPFIYSMIGG
AVGGLYLMINNVTAYTMGGLGIFGVLNFINGDDASGMIQSFIAIAIAAVVGFGLTFFFWKDNTVEEEEVIIDKTTIKKENI
TSPVKGRVLSLKNAEDPAFANGALGNGVVIEPTEGKVVAPFDGTIVTLFPTKHALGLISDNGTELLIHIGIDTVQLEGEGF
EAFVKQGDRVKKGQTLVTFDLEGIKKAGFSTQIPIVVTNTADYLDILEVGSNEVSTSDDLLTALI
Seq ID 178
MESKTFDIEGMSCASCAQTIEKATAKLPGMAKASVNLATEKLSVTYDQTEVTEEEIKEAVSDAGYKAISPAQQRTFAIEGM
SCASCAQTIEKAVNQLSGVQQAIVNLATEKLVVSYDDHQVTSAEIIKAVTDAGYQATEEVAAGATADQDREKKQKHIAEMW
QRFWISAVFTVPLLYIAMGHMVGLPLPDFLNPMTHAMTFAMVQLILTLPVLYVGREFFTVGFKALFKGHPNMFSLVALGTS
AAFVYSLYGTVMIFLGDTSFTMALYYESAGVILTLITLGKYFEAVSKGKTSDAIKKLMGLAPKTAHILRDGVEIEVPVDAV
QLDDIVIVRPGDKTPVDGVIVSGSSSVDEAMLTGESLPVEKKVGDAVIGASINKNGSFQFKATKVGKETALAQIIQLVEDA
QGSKAPIAQLADKISGVFVPIVIGLAVLSGLAWFFLGQESWIFALTITISVLVIACPCALGLATPTAIMVGTGKGAENGVL
IKSGDALETTHKIQTIVFDKTGTITEGKPVVTDILVADSALSEAELLTLAASAEQGSEHPLGEAIVGAAKERQLPLAEGSD
FSAIPGHGIRVTVNERVLLLGNIKLMKEEAIELSTFVQQADRLAEEGKTPMFVAKDGSFAGIIAVADTVKDSSQTAIARLH
KMGIEAVMITGDNKRTAEAIAKQVGIDRVLSEVLPEDKALEVKKLQAEGKKVAMVGDGINDAPALAQADVGIAIGSGTDVA
MESADIVLMRSDLMDVPTAVELSKATIKNIKENLFWAFAYNTLGIPVAMGVLHLFGGPLLSPMIAAAAMSFSSVSVLLNAL
RLKGFKPSTVKRTSGSQK
Seq ID 179
MKKKILAGALVALFFMPTAMFAAKGDQGVDWAIYQGEQGRFGYAHDKFAIAQIGGYNASGIYEQYTYKTQVASAIAQGKRA
HTYIWYDTWGNMDIAKTTMDYFLPRIQTPKNSIVALDFEHGALASVPDGYGGYVSSDAEKAANTETILYGMRRIKQAGYTP
MYYSYKPFTLNHVNYQQIIKEFPNSLWIAAYPIDGVSPYPLYAYFPSMDGIGIWQFTSAYIAGGLDGNVDLTGITDSGYTD
TNKPETDTPATDAGEEIEKIPNSDVKVGDTVKVKFNVDAWATGEAIPQWVKGNSYKVQEVTGSRVLLEGILSWISKGDIEL
LPDATVVPDKQPEATHVVQYGETLSSIAYQYGTDYQTLAALNGLANPNLIYPGQVLKVNGSATSNVYTVKYGDNLSSIAAK
LGTTYQALAALNGLANPNLIYPGQTLNY
Seq ID 180
LPMIIKQEQFIPKDTVETTIDLLIRNLTTIKDNTGEFLLDFDGLKVDDKSWTIWNWPQGVGLYGIYKNYRNTKSEKALQVV
NDWFEGRMQEGAPPKNVNTMAPLLTMAYLYEDTKDSKYIPYLEQWAEWVMEEMPRTNEGGLQHATYGPENKNQLWDDTLMM
TVLPLAKIGKLLNRLDYLEEAKHQFLIHIKYLQDKKSGLWYHGWTFEGNHNYAEALWARGNCWITIAIPEIIEILELPKGD
SLREFLLSTLNAQVAALAKYQDESGLWHTLINDSNSYLESSATAGFAYGILKAVHKKYISSEYEEVANKAIAGLLNEIDET
GEGSVGTGMGDNLDFYRTIGMTAMPYGQSLTILCLTELLVSYC
Seq ID 181
MKAKKQYKTYKAKNHWVTVPILFLSVLGAVGLATDNVQAAELDTQPETTTVQPNNPDLQSEKETPKTAVSEEATVQKDTTS
QPTKVEEVAPENKGTEQSSATPNDTTNAQQPTVGAEKSAQEQPVVSPETTNEPLGQPTEVAPAENEVNKSTSIPKEFETPD
VDKAVDEVKKDPNITVVEKPAEDLGNVSSKDLAAKEKEVDQLQKEQAKKIAQQAAELKAKNEKIAKENAEIAAKNKAEKER
YEKEVAEYNKHKNENSYVNEAISKNLVFDQSVVTKDTKISSIKGGKFIKATDFNKVNAGDSKDIFTKLRKDMGGKVTGNFQ
NSFVKEANLGSNGGYAVLLEENKPVTVTYTGLNASYLGRKITKAEFVYBLQSSPSQSGTLNAVFSNDPIITAFIGTNRVNG
KDVKTRLTIKFFDASGKEVLPDKDSPFAYALSSLNSSLTNKGGHAEFVSDFGANNAFKYINGSYVKKQADGKFYSPEDIDY
GTGPSGLKNSDWDAVGHKNAYFGSGVGLANGRISFSFGMTTKGKSNVPVSSAQWFAFSTNLNAQSVKPIFNYGNPKEPEKA
TIEFNRYKANVVPVLVPNKEVTDGQKNVNDLNVKRGDSLQYIVTGDTTELAKVDPKTVTKQGIRDTFDAEKVTIDLSKVKV
YQADASLNEKDSKAVAAAINSGRAKDVTASYDLHLDQNTVTAMMKTNADGSIVLAMGYKYLLVLPFVVKNVEGDFENTAVQ
LTNDGETVTNTVINHVPGSNPSKDVKADKNGTVGSVSLHDKDIPLQTKIYYEVKSSERPANYGGITKEWGMNDVLDTTHDR
FTGKWHAITNYDLKVGDKTLKAGTDISAYILLENKDNKDLTFTMNQALLAALNEGSNKVGKQAWSVYLEVERIKTGDVEVT
QTENYNKELMRSNTVVTHTPDDPKPTKAVHNKKGEDINHGKVARGDVLSYEMTWDLKGYDKDFAFDTVDLATGVSFFDDYD
ETKVTPIKDLLRVKDSKGVDITNQFTISWDDAKGTVTISAKDPQAFILAHGGQELRVTLPTKVKADVSGDIYNSAEQNTFG
QRIKTNTVVNHIPKVNPKKDVVIKVGDKQSQNGATIKIGEKFFYEFTSSDIPAEYAGVVEEWSISDKLDVKHDKFSGQWSV
FANSNFVLADGTKVNKGDDISKLFTMTFEQGVVKITASQAFLDAMNLKENKNVAHSWKAFIGVERIAAGDVYNTIEESFNN
ETIKTNTVVTHTPEKPQTPPEKTVIVPPTPKTPQAPVEPLVVEKASVEPELPQTGEKQNVLLTVAGSLAAMLGLAGLGFKR
RKETK
Seq ID 182
MKKILFASLFSATLLFGGSEISAFAQEIIPDDTTTPPIEVPTEPSTPEKPTDPTPPIEPPVDPVEPPITPTEPTEPTEPTT
PTEPTTPTEPSEPEQPTEPSKPVEPEKPVTPSKPAEPEKTVTPTKPTESEKPVQPAEPSKPIDVVVTPTGELNHAGNGTQQ
PTVPIETSNLAEITHVPSVTTPITTTDGENIVAVEKGVPLTQTAEGLKPIQSSYKVLPSGNVEVKGKDGKMKVLPYTGEEM
NIFLSAVGGILSVVSGFVIFKKRKAKV
Seq ID 183
MGSRFGLMLKQGGNDVLLIDGWQEHINAIKENGLKANYNGEEITVKVSIVNQNEVPTGEQFDLIILFTKAMQLEKMLQDVK
PLIADHTEVLCLLNGIGHEDVIEKFVPMEKIFIGNTMWTAGLEGPGKAKLFGSGSVELQNLGIGQEESAKKLAETLSASGL
NAKYSDNIHYSIYRKACVNGTMNGLCTILDVNMAGLGATKPAHDMVVTIVNEFAAVAAKENVNLDIPEVIEHVETCFDPTT
IGMHFPSMHQDLIKNNRLTEIDYINGAISRKGKKYGVVTPYCDFLTQLVHSKEEILGAK
Seq ID 184
MQTTEEMQSGDGRRGRLRIFFGFAAGVGKTYGMLTEAHELLMMGKHVVVGYIEPHDRPDTNRLLEGLPQIPPKNIFYKQMV
LTEPDIDQIIQQKPEIVLIDELAHSNAEGSRNRKRYQDIDELLNAGIDVFTTVNVQHIESLNDIVEEVTGIEVKETVPDTF
LRQATIRVIDVEPDELIERLEQGKIYANENAKRALKNFFIPQKLDQLRGLAIQRASDHINRISGKTIGIQSKLLTVVNDAF
PKMTEKCIRWTARLAQGLVVDWTVIQVRTQENTPTNIPLADKLGAEVISIEEDDSFETIVEFAKMTGVTDIIMGKNLRQPW
YEKIFIEAFDDRLLKRLKDTELHLIPFNEEKRSLFFKTRKVIEGGGKDLVIAIGGVFLATVVTELMQYIHVGDQNLMLIYI
SFVLLVARTTSGYFWSSLSSILSVLSFNWFFVEPLYSLTVYKQGYPFTLLLMLVVALMSSNLMIRLKKQADTSMKKEHQME
ILYELNKRYVLVESRKQILDISATYLSRLLEREVIIFDRQVKTESVHCINEKKSILNNEDEAAVAFWAAKNQKEAGNGTDT
LNGAKGFYLPIAAGRKTLAVLGIERNADLDLENDQLNYLKLVLTQIAVILEQTELKDEKEQVELENEREKVRSNLLRAVSH
DLRTPLTVISGIAETLGIGNDLKEETQRKLLKDIQEESQWLIRMVENLLSITRINMDTMKVNKTAEPVEEVIEAVYKHLRK
VYPDGQVDIHLPEEVIFIQADPILIEQALFNLIENAFRHGENDLPVKLNVYQEKEQTVFEIENHGEIPLKQFQKIQSNLSG
TNEVPVDSKNGLGIGLSIVKTIVHAHNGKMEMTIGRGKTLVRIYLK
Seq ID 185
MKNFYKKKFALTDQGAEALTKASISSFFVYCINMVPAFIIMMLIDELVLENAKPRWLYFAVSFVTLLFMYWLLDREYENLY
NSTYKESAHLRVQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKSGGMALFFPFISVMLLVGNVKMGLAV
ILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVM
ALSSIFSYVSLAVVILVGVHLLLTGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
LKSFDVELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDEGCILIDGYDIKRVSPASLFSK
IAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAP
ILILDEITASLDAENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKVVDQGTHSELYRRSEIYKNLIKKTKL
SEKFVYEKEAQSR
Seq ID 186
LTLSDTLGQREVTALSYDSRDVTAETLFFCKGLNFKEIYLENAVKDGLEIYVSEVPVEVPAQLGIIVTDIKKAMAVLSMAF
YDYPQNKLKLIGFTGTKGKTTAAYFTKYILDVATQQKTALLSTMNSTLDGKTFFKSALTTPESLDLYRMMATAVANGMTHF
IMEVSSQAYKTNRVYKLFFDVGIFLNITPDHISPIEHPTFDDYFYCKRQLITHSKVIVLNHEADYFPLLKETAQQQKVPAI
VYGSQPAPEVDYSFAVSSEDSLRFIVESPADALGLAGSYHLRLGGDFNKGNALSAAIASVLVGASKEECQQGIAATTVPGR
MESLTNTNGATVYVDYAHNYDSLKNLLTFVREEHPDGRLIVLVGSTGDKAISRRKDFGRVLSELADVAVLTTDDPASEDPA
KICQEIQAHITKEMPVYTVLDRGEAIAHALSLSTTADDAIVLAGKGADLYQKVNGVDEPYAGDFALAEAFINKKN
Seq ID 187
MFLGFNEMKYSKGRYVLVVLVMVLIAWLIFILSGLANGLAQGNRLAVDQWQANQVVLSKEANSNLNVSVLDENVKETISGG
KIAPIGQQSLAIRPADDKKAELTNVSLFGIEKESFLMPKVIEGNAFTDKNGVIASETLKNQGFKIGDKLTAGKYDEQLEIV
GFISKSSYNIVPVIYTSLDTWRSIKYGDNPAMAKMVNGFIVRSKDNTEVKTTNKDSQVLSISDFIEKLPGYSAQNLTLDGM
IYFLIVIAAFIIGIFIFVMTLQKTAMFGVLKVQGVPTSFLAKAVMLQTALLAVLGVAIGLALTGITVLFLPEAMPYATNGP
RMILFSVLLILSALIGGAFSIRTIAKIDPLIAIGG
Seq ID 188
MQIFPYSDDPNKRYHTWNYALRQQFGEKIFKVPIDGGFDCPNRDGTVAKGGCTFCSVSGSGDMIVAPSDPLPLQFQKEIQL
MHQKWPTVDQYIVYFQNFTNTHAPVDVIRHRFEQVVNEKGVVGLSIGTRPDCLPDEVVNYLAELNERFYLWVELGLQTTFE
ETSAAINRAHDYQTYLDGVAKLRKHGIRVCTHLINGLPGETPAMMRENVRRTIQDSDIQGIKLHLLHLMTNTKMMRDYNEG
RLQLMSKEAYVSVICDQLEMIPPEIVIHRLTGDAPFETIIGPMWSLKKWEVLNAIDAEMKRRNSYQGKYTVISGKEVFN
Seq ID 189
MKKESMSRIERRKAQQRKKTPVQWKKSTTLFSSALIVSSVGTPVALLPVTAEATEEQPTNAEVAQAPTTETGLVETPTTET
TPGTTEQPTTDSSTTTESTTESSKETPTTPSTEQPTADSTTPVESGTTDSSVAEITPVAPSATFSEAAPAVTPDDEVKVPE
ARVASAQTFSALSPTQSPSEFIAELARCAQPIAQANDLYASVMMAQAIVESGWGASTLSKAPNYNLFGIKGSYNGQSVYMD
TWEYLNGKWLVKKEPFRKYPSYMESFQDNAHVLKTTSFQAGVYYYAGAWKSNTSSYRDATAWLTGRYATDPSYNAKLNNVI
TAYNLTQYDTPSSGGNTGGGTVNPGTGGSNNQSGTNTYYTVKSGDTLNKIAAQYGVSVANLRSWNGISGDLIFVGQKLIVK
KGASGNTGGSGSGGSNNNQSGTNTYYTVKSGDTLNKIAAQYGVSVANLRSWNGISGDLIFVGQKLIVKKGASGNTGGSNNG
GSNNQSGTNTYYTIKSGDTLNKIAAQYGVSVANLRSWNGISGDLIFAGQKIIVKKGTSGNTGGSSNGGSNNNQSGTNNTYY
TIKSGDTLNKISAQFGVSVANLQAWNNISGSLIFAGQKIIVKKGANSGSTNTNKPTNNGGGATTSYTIKSGDTLNKISAQF
GVSVANLRSWNGIKGDLIFAGQTIIVKKGASAGGNASSTNSASGKRHTVKSGDSLWGLSMQYGISIQKIKQLNGLSGDTIY
IGQTLKVG
Seq ID 190
MMIVVLLMSSCLFLFVSYQLYLRLLVEKLDSEDRTLKRKINFYKYQKNSRLMIYLLFSVCLLSVLLLGIVYTYYQLNQRNI
RMEQRIERLAQGQATQGDKIKKQAIKKTALNQFPWKQAVSAESAAVLTNYELQLAREWRPYLGETSITMIRSEKTQTLTLS
VFSVGLSYHEFQTGQDNIVALIAALNSVKEITMIDFNFTYRDQEQTLVKSIDTYARETLETNLEPVVIS
Seq ID 191
MSNYTIIDLNQQPHLIPSAAQWFSQKWQIPAEAYQASMQEMLTSENHVPHWWVCLNKQQDIVAGVGVIDNDFHNRADLTPN
LCALYVEPTFRHQGLAGILLTTVGDFLANSGFKKLYLLTDHTTFYERYDWEFLTMVTTEEQSCARIYQKQLRQ
Seq ID 192
MKNSENDYIQSLFQILPGLLTAFLVACLSKFLAIWLPSLGAATIAILLGIFLGNTFVRGANLNRGTKVAESKLLEFSVVLL
GTTVTFQTIAQIGLQGVAFILIQMSLTIIFAYLIGKKLAFSDNMSLLMAGGNAVCGSSAIASIAPAIQADEEEKGQIITLV
NLLGTVLMLTLPILSGILYGTNLLARSALIGGTLQSVGQVVASANMVNENAVQLAMLFKIMRIVLLVAVVYLFGRFKQSKT
AESFAELVEVTKKSSALPWYVVGFFIACVFNSLIHFPVVISETAHFFSSWFEITALAAIGLRLDFKKFFQEGKRFLIYGLS
VGTVQVVLAILLLALLQF
Seq ID 193
MAKTGMYVGLDIGTTSVKVVVAEYIEGQMNIIGVGNAKSDGLNRGIVVDIDQTVQAIQRAVRQAEEKAGIQIKSVNVGLPA
NLLEVESCQGMIAVSSESKEITDEDVRNVASAALVRSTPPERQIVAILPQDFTVDGFEGIKDPRGMLGVRMEMFGVVYTGP
KTIIHNIRKCVEKAGLGINELVITPLALTETILTDGEKDPGTIVIDMGGGQTTTSVIHDKQLKFTHVNQEGGEFITKDISI
VLNTSFNNAEALKINYGDAYPERTSANEEFPVDVIGKSEPVRVDERYLSEIIEARVEQILRKSKEVLDEIDAFELPGGVVL
TGGAASMPGIVDLAQEIFEANVKLYVPNHMGLRNPVFANVISIVEYSAQLNDIYHIAKYAIPGEKSKPAQSVAVQQ
EVRYDTYAEQPQEEYEEFNERESGEKVTGKIKDFFSNIFD
Seq ID 194
MAKKTIMLVCSAGMSTSLLVTKMQKAAEDRGMEADIFAVSASEADTNLENKEVNVLLLGPQVRFMKGQFEQKLQPKGIPLD
VINMADYGMMNGEKVLDQAISLMG
Seq ID 195
MKKEIIVYTISDSLGETSQKLLAAASAQYPDISFLNRYNFSFVTTEEELLEILKDALKDKALVVSTLVSKQLITAAKEFSE
RTGLLYLDLMAPFFELIQAKAGVDPIEEPGRRHQLDRAYFDKISAIEFAVKYDDGKNPQGFLDSDILLLGVSRTSKTPVSM
YLANQGYRVSNLPLIPEVPLPPILEEMDPQKMIGLVCSPETLGQIRSSRLASLGLGNETSYTNVERIEQELAYAEEIFAKY
GIPVIDVTAKSVEETAFLIKEKLDERN
Seq ID 196
MEWIEIKHATQNNLKNISVNIPKKQLTVVTGLSGSGKSSLVFDTLAAESRRELNDTFSSFVQNYLPKYGRPEVEKIENLPV
AIVIDQKKVAGNSRSTVGTYTDIYTFLRLLFSRAGSPFVGYSDTFSFNHPDGKCPTCDGLGKITEINLHQLVDYDKSLNKG
PIDFPTFTVGNWRWKRYAHSGLFDLDKKIKDYSPEELALFLYAPQQKLANPPKEWPHTALYEGIVPRMQRSILHTDEGKRH
QKYLNHFVTVKRCPDCLGSRVNERVRSCKINQKSIADAVDMPLTELHSFIRSMDLSLIKTIQEELLVRLEALINIGLSYLT
LGRATETLSGGEAQRIKIAKYVNSALNDIMYILDEPSAGLHPKDIERISVALLNLKNKGNTVVLVEHNPQLIREADFIIDI
GPFAGENGGHVQFSGTYDAFLVSKTLTSQALQEPLPLNDQPRKARKSLSIEHATLHNLNNLSVEVPLGVLTVICGVAGSGK
SSLAEEIYQKAQADNQEIIHLSQKSITANLRSTPMTYLNIFDKVRKLFAEENHVSPALFSYNSKGACPTCKGKGIIVSDMS
FMEDVTSICETCHGTRYKEEVLHYLYNGKNIVEVLALSVKDGYDFFKDQPFALSLKNLLEVGLSYLKLNQSLSTLSGGELQ
RVKLADTLHQKKAIYLMDEPTDGLHLIDIQQSLQLFNRMVEEGNSLILLEHHIDVIKSADWLIELGPEGGKNGGQLLFTGT
PANMLNSTHSITKGYL
Seq ID 197
MKKLKMLGCVGLLLALTACQAGTGNSADSNKAAEQKIAISSEAAISTMEPHTAGDTTSTLVMNQVYEGLYVLGKEDELELG
VAAEEPAISEDETVYTFKIREDAKWSNDDPVTANDFVYAWQQVASPKSGSIHQALFFDVIKNAKEIALEGADVNTLGVKAL
DDKTLEITLERPTPYLKSLLSFPVLFPQNEKYIKEQGDKYATDAEHLIYNGPFKLKEWDNASSDDWTYEKNDTYWDAEKVK
LTEAKVSVIKSPTTAVNLFDSNELDVVNKLSGEFIPGYVDNPAFLSIPQFVTYFLKMNSVRDGKENPALANNNIRKALAQA
FDKESFVKEVLQDQSTATDQVIPPGQTIAPDGTDFTKLAAKKNNYLTYDTAKAKEFWEKGKKEIGLDKIKLEFLTDDTDSA
KKAAEFFQFQL
EENLDGLEVNVTQVPFTIRVDRDQTRDYDLELSGWGTDYRDPLTVMRIFTSDSTLGGVTFKSDTYDQLIQETRTTHAADQE
ARLNDFAQAQDILVNQETALAPIYNRSISVLANQKIKDLYWHSFGPTYSLKWAYVN
Seq ID 198
MKQLKKVWYTVSTLLLILPLFTSVLGTTTAFAEENGESAQLVIHKKKMTDLPDPLIQNSGKEMSEFDKYQGLADVTFSIYN
VTNEFYEQRAAGASVDAAKQAVQSLTPGKPVAQGTTDANGNVTVQLPKKQNGKDAVYTIKEEPKEGVVAATNMVVAFPVYE
MIKQTDGSYKYGTEELAVVHIYPKNVVANDGSLKKVGTAENEGLNGAEFVISKSEGSPGTVKYIQGVKDGLYTWTTDKEQA
KRFITGKSYEIGENDFTEAENGTGELTVKNLEVGSYILEEVKAPNNAELIENQTKTPFTIEANNQTPVEKTVKNDTSKVDK
TTPSLDGKDVAIGEKIKYQISVNIPLGIADKEGDANKYVKFNLVDKHDAALTFDNVTSGEYAYALYDGDTVIAPENYQVTE
QANGFTVAVNPAYIPTLTPGGTLKFVYFMHLNEKADPTKGFKNEANVDNGHTDDQTPPTVEVVTGGKRFIKVDGDVTATQA
LAGASFVVRDQNSDTANYLKIDETTKAATWVKTKAEATTFTTTADGLVDITGLKYGTYYLEETVAPDDYVLLTNRIEFVVN
EQSYGTTENLVSPEKVPNKHKGTLPSTGGKGIYVYLGSGAVLLLIAGVYFARRRKENA
Seq ID 199
MKTPTFGKREETLTYQTRYAAYIIVSKPENNTMVLVQAPNGAYFLPGGEIEGTETKEEAIHREVLEELGISVEIGCYLGEA
DEYFYSNHRQTAYYNPGYFYVANTWRQLSEPLERTNTLHWVAPEEAVRLLKRGSHRWAVEKWLAAAS
Seq ID 200
MARVESFELDHNTVKAPYVRLLGTEQNGDALVEKYDLRFLQPNKDALPTGALHTLEHLLAVNMRDELKGIIDISPMGCRTG
FYMIMWDQHSPQEIRDALVNVLNKVINTEVVPAVSAKECGNYKDHSLFAAKEYAKIVLDQGISLDPFERIL
Seq ID 201
MNIFAVRLKEALTAKNIKPSDLAKKTGIGKSSISDWLAGRYEAKQDKVYRIADALDINEAWLMGQEVPMEKNASTIDRIYK
KLEPQRQAIVYQFAEQQLHEQQTQAEILSFPRRDEMTLAAHAGDPEKIFSKEEIEKIHDYLDEIDAKYQQSISSDKKED
Seq ID 202
MYKFVDTNQATHSTPLPSEALNFNGQFLEKVIPGYQTLSVSGRELVPSEIESYQLGIRDGKRHVYARIPERELTVKYRLSA
VNNEAFRDAFNHLNVALFTEKDVSIWFNDEPEMLWFGSKSSVSDVPEGVNQVTGTFTLLLSDPYKYTRSDATSVMWGSPTI
TFQANYLMGNTGSGAFDFPILIEGGAYWGSTMITFQNRAYTMGDLGKEVRPIEIYPTVEGLKVKPTIILTGTGRGVWIKTR
NDTINLGDFDRSEIIIDTENFYLTKNGAPMIRPMNDFYLYPNEPLYIQAKDSDFRLTIRYPNRFV
Seq ID 203
MDFFSSEEIVASLGDKAEMKGKLSFVRLCILGIMAGFCIALGYLAFIRITGTAPTEWGSFNNFLGGALFPVGLIALTFLGG
ELATGNMMVMTLGVLQKKVRVGALCYNWIVVLLTNCLGGFLVAYLFGHIVGLTEGAFLDKTVAVAQAKIADPPLVAFVSGI
GCNIFVCLAVYLGALAKSYLGKMFGLWFPVMVFVVCGFQHVVANAFIIPAAIFSQSTTISWWDYLQNTLWVFLGNAVGGSL
FMAVPLIFMTKPATVKPRVEKTIQTEELYGN
Seq ID 204
MNQRILSTLGFDKVKQQLLQFIVTAQGTNEVSELLPIADENKIQSWLNETQDGLKVQRLRGGIPIPKLENIQPHMKRIEIG
ADLNGIELAQVGRVLSTTSELTRFFDELSENEVDFERLYMWREQLEVLPELNRQLKQAIDDDGYVTDEASPALKAIRQNIR
RSEQTIREELDSIIRGKNARYLSDALVTMRNERYVIPVKQEYKNIFGGVVHDQSASGQTLFIEPKQILEMNNRLRQQQIAE
RNEITRILAELSAELVPYRREITHNAYVIGKLDFINAKARLGKELKAVVPEISQANHVVFKQARHPLLNPEKAVANDIVIG
EEYQAIVITGPNTGGKTITLKTLGLLQLMGQAGLPIPVEEESKMGIFTEVFADIGDEQSIEQSLSTFSSHMTNIVSVLKKV
DHQSLVLFDELGAGTDPQEGAALAIAILDSLGAKGAYVMATTHYPELKVYGYNRAGTINASMEFDVDTLSPTYRLLIGVPG
RSNAFEISKRLGLDNSIIEAAKQIMDGESQDLNEMIEDLENRRKMAETEYLEARHYVDESAALHKELKEAYQVFFEFREKE
LQKARKEANKIIAEAEENAETIISDIRKMQLESGQQGGVKEHQLIDAKTQLSQLHHEETKLAKNKVLKKAKEQKKLKAGDE
VIVNTYGQRGTLLKDNGKGQWQVQLGILKMNVSEEDMTPVAPQKEAKPRVTTVRSAESSHVGTQLDLRGKRYEEALAEVDQ
YIDAAILAGYPQVTIVHGKGTGALRTGITEFLKNHRSVKSYEFAPQNQGGNGATVVKFQ
Seq ID 205
MKEITGATRLAGLFAKPSQHSISPLIHNTAFQNLGVDARYLAFDVGQETLPQAIEAIRTFHMLGANLSMPNKVAAVSYMDE
LSPTAQLVGAINTIVNKDGKLYGDSTDGTGFMWSLKEKKVDVFQNKMTILGTGGAALSIIAQAALDGVKEIAVYNRKSAGF
NDSQKKLANFTERTNCVIHLNDLADTEKLAKDVAESVLLVNATSVGMHPHAHSSPIENYAMIQPKLFVYDAIYNPRETQLL
KEARLRGAETSNGLDMLLYQGAAAFEQWTGQKMPVSVVKRKIENR
Seq ID 206
MTKGYVKSVTELIGQSPVVKLKRMVPEGAADVFVKLEFFNPGGSVKDRIALSMIQQAEADGRLKPGQTIIEPTSGNTGIGL
AMVGAALGYPVKIVMPDTMSIERRKLMQAYGADLLLTPGAEGMKGAIAKATALAEEHGYFMPLQFNNPANPMVHEQKTGKE
IVDVFGKRGLDAFVSGVGTGGTVTGVGHELKRIFPDIEIVAVEPTESPVLEGGEPGPHKIQGIGAGFVPEVLDTTVYQKVA
AVSSEDALETARLMGPKEGILVGISAGAAIKAAIDLAVELGAGKRVLALVPDNGERYLSTALYEFPE
Seq ID 207
VKNQHLTTSQGSPVGDNQNSLTAGEFGPVLIQDVHLLEKLAHFNRERVPERVVHAKGAGAHGIFKVSQSMAQYTKADFLSE
VGKETPLFARFSTVAGELGSSDTLRDPRGFALKFYTDEGNYDLVGNNTPIFFIRDAIKFPDFIHSQKRNPRTHLKSPEAVW
DFWSHSPESLHQVTILMSDRGIPLSFRHMHGFGSHTFKWVNAAGEVFFVKYHFKTNQGIKNLESQLAEEIAGKNPDFHIED
LHNAIENQEFPSWTLSVQIIPYADALTMKETLFDVTKTVSQKEYPLIEVGTMTLNRNPENYFAEVEQVTFSPGNFVPGIEA
SPDKLLQGRLFAYGDAHRHRVGANSHQLPINQAKAPVNNYQKDGNMRFNNGNSEINYEPNSYTETPKEDPTAKISSFEVEG
NVGNYSYNQDHFTQANALYNLLPSEEKENLINNIAASLGQVKNQEIIARQIDLFTRVNPEYGARVAQAIKQQA
Seq ID 208
MAENYQQAAKDIIQLIGMDNIISVTHCQTRLRFILKDHEQVDGKQLEKIDLVKGVFYNGGQYQVILGTGIVTKVYDEIEKL
GINVVSKAEQTAILKNNETGMRKTMRILSEIFIPIVPVIAATGLFLGLKGVIFNDTFLQLFGASVANIPESFQQIVSVITD
TVFAFLPALIVWSTFKAFNATPVIGIVIGLMMVSPILPNAYAVATPDSGVKAIMAFGFIPVVGAQGSVLSAIAAGIIGAKI
ELFFRKKMPNILDQIFTPFMTMLITFLIMILGIGPILHTVELGMVDVVQWLIGLPLGLGGFVIGASYPLMVLIGIHHTLTM
VETSLLANTGFNALITICAMYGFANVGSCLAFAKKAQDSKVKSTAIGSMLSQLFGVSEPVLFGLLIRWNLKPLLCVLFTSG
LGGAILAIFHIQSNSYGLAVIPSFLMYIYSAHQLVIXLLVALLSVGVCXALTSLFAIPQEVLISDKVIEEEEREVFEMQHN
TLDEQLFSPVTGYAMNLTAVNDPVFSSEMMGKGLAIMPTANKVYAPADGLLNLVAETGHAYGIQTDAGAEVLIHIGIDTVT
LGQEVFQTQVTQGHRVKKGDLLGTFDRKAIKKAGLDSTVMVIITNTSSYLSVEPMMSDHNEITPEQIILNLNTPN
Seq ID 209
VELVDKDLRSIQETRNLIRKAKEAQQVLATFSQKQIDAIVQAVSEATFNQREKLAKMANEETGFGIYEDKIIKNAFASKVV
YDEMKDKATVGVIHDDAAKKVTEIAVPVGVIAGLIPSTNPTSTVIYKALISLKAANSIVFSPHPNALKSIIETVEIIQKAA
IAAGAPEGCVSVIKTPTMQATSELMKNKETNLILATGGNAMVKAAYSSGTPAIGVGPGNGPAYIERSANVPHAVKQIMDSK
TFDNGTICASEQSIIVETVNREAVKEELIKQGAYFLSPAEADKLAKFILRPNGTMNPQIVGRSVQHIASLVGLSIPKDRRL
IVAEETHVGLKYPFSREKLAPIIAFYTVENWEAACALSIEILKGEGAGHTMGIHTENKEVIREFGLRKPVSRLLVNTSGTL
GGIQASTNLVPALTLGCGAVGGSSTSDNIGVENLFNLRRVAYGVRDLEEIRQEFGQTSTTSVATSCETTNQEELVNAVVAQ
VLARLN
Seq ID 210
MNELNKTPKEIVKELDQYIVGQQAAKKSVAVALRNRYRRLQLEENMQQDITPKNLLMIGPTGVGKTEIAPRLAKIVNAPFV
KVEATKFTEVGYVGRDVESMVRDLVENAIQIVEKQQYSRVYAQALKKANQRLVKVLVPGIKKEQKQAGGNQFEQMMNMFNM
AQQQQEAQEEVTEDIRTNRRTILEQLEKGLLDNREVTIEIEEPKKTMPAMNNGLEQMGIDLNETLGALSPKKKIERTVTVK
EAQELLVKEESAKIVNDADIHSEAIRLAESSGIIFIDEIDKITSKSQQNSGEVSREGVQRDILPIVEGSQVNTKYGPLQTD
HILFIASGAFHISKPSDLIPELQGRFPIRVELDDLTADDFVSILTEPNNALIKQYVALIGTENVSVIFTKEAIERLAHIAY
DVNRDTDNIGARRLHTILERLLEDLLYEAPDMQMGEITITEAYVNEKLNDIVQNEDLSRYIL
Seq ID 211
MAKRIIIMNFDIESKSYQAFSEIKKMQAERQL
KGEQMAVVTHVNDGQHQFKINDFIDFTGNNHTSKDSMIGMLVGILGGPLGILFGWFAGSMYGASKDAKEIQEAQTVFEHVI
QKIDEGQTGLLLIAEEEDNRPLNQLVMFDLGGEITRLDLEEVQQEINDANEVANEAKQSWQAKKEQHKEATSKEE
Seq ID 212
MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVIDRLDYRPNAVARGLASKKTTTVGVIIPDVSNAFFASL
ARGIDDVATMYKYNIILANSDGDDQKEVTVLNNLLAKQVDGIIFMGHRITDDIRGEFSRSKTPVVLAGSIDPDEQVGSVNI
DYTEATKDATATLAKNGNKKIAFVSGALIDPINGQNRMKGYKEALAENGLSYNEGLVFESEYKFKAGINLAERVRNSGATA
AFVTDDELAIGLLDGMLDAGVKVPEEFEIITSNNSLLTEVSRPRLSSITQPLYDIGAVSMRLLTKLMNKEEIEEKTVVLPY
GIDQKGSTK
Seq ID 213
LKKYNVDWNYWVVRFLFVMALIVGYLLITNYQHFVHSVSGLLGILSPFITGFVIAYLLSGSQKKIEGLLERVRLPVVKKAK
HGLSVLLLYLIILFIFVLTLNYIVPLLISNLVDLANSLPTFYDHMVQFVMSLEDKGILKTAAIEKYLNSVLKDLSPERFLN
QWTQALFSLGTLTKNVSSFFLNAFLTLIISIYALVFKQSILTFVEKAAHKLLSEKVYKQTQTWLNTTNKIFYKFISCQFLD
ACIIGVSSTILLSILNVKFAVTLGILLGICNMIPYFGSIFASIVAGVITLFTGGVTQAITVLLVLLILQQIDGNIIGPRIM
GDALNVNPILIIVSITIGGAYFGVLGMFLAVPVAAIIKIIVSEWLNESKENDKIVDSIES
Seq ID 214
MKKFSIRKISAGFLFLILVTLIAGFSLSANAEEYIVPAESHSRQKRSLLDPEDRRQEVADTTEAPFASIGRIISPASKPGY
ISLGTGFVVGTNTIVTNNHVAESFKNAKVLNPNAKDDAWFYPGRDGSATPFGKFKVIDVAFSPNADIAVVTVGKQNDRPDG
PELGEILTPFVLKKFESSDTHVTISGYPGEKNHTQWSHENDLFTSNFTDLENPLLFYDIDTTGGQSGSPIYNDQVEVVGVH
SNGGIKQTGNHGQRLNEVNYNFIVNRVNEEENKRLSAVPAA
Seq ID 215
MKRKKIKNQLLVLTSILVLLSLEVAPVVTLAAELPSSSLQTALSSETTITSEEKVTETTETTVATSTTSTSSSSSESSSST
DTTTESTSQSTTETTTTNTSSETKKEPTEPTVSSEITQPVEQSQPPQVPVTKQEPEEPIQVPEANNNFVEENQAVSLNPSL
KVDEIASSNLKGYELPLLSSFGEKKRAVVVAEALRHVGKTKKEFNLTEQALTSSFLAQKIYQQLFKIDIGSTPQEQMTFGK
VRSIEEAEPGDLIFWQTAEGKTLQNGVYLGQGKYLIAAAESDSKEKPEVIAQLENIYTAKQQPDSKEEKRLVVTNPFKEFI
LTEYGKEVLATYGASFEMQKSEQTTAFIKKIGETARELGEKYDVFASVMIAQAILESGSGESQLAKEPYYNLFGVKGSFQG
NSVSFSTKEADQRGQLYTISAGFRDYGGYNDSLQDYVQLLRQGIDGNQDFYKPAWRSEAKNYLQATRFLTGKYATDKQYDN
KINSLIAVYNLTQFDLPKTVDGLIIQSKNKLSEAEQQQMHFPVYDGINYNRSGSYPVGQCTWYVYNRFKQLGTSVDEFMGN
GSDWGRKGRALGYQVSSLPKAGRAISFQPGVAGADNQYGHVAFVEA7TSDGIIISESNVINDQTISYRVLPNVIAYSSGVT
YIGA
Seq ID 216
VDAKAYLGPDDEAAHSFGLRKTPRLQAMYDKPGTIYLYTMHTHLILNMVTQEQGKPQGVMIRAIEPVEGVDKMIENRQGRQ
GVELTNGPGKLVAALGIDKQLYGQSIFSSSLRLVPEKRKFPKKIEALPRIGIPNKGRWTELPLRYVVAGNPYISKQKRTAV
DQIDFGWKDEENEKSNNAHILRGTT
Seq ID 217
MAKKKNVSVISVEKPTWFPLTDETGAFPVYGAPITIGTAVSIKPDVTTETTPDYGDSVVQDQYVAFGGAEVTLETNGYQNE
VLAEITGGKKLKGGVLRSADDIASDGAFAYRRRKSNGKYRYTIFYKGKFALTSDETSTLEGSSVSYTHPEWTGSFVDVPGL
GYMYSVDEDDEGVDLEMIKNWFTEVMDPRKENTTAVTGVTLDQTELNLKVGQTATLTPTITPDNASNKKYQFRSESEAIGT
VTPIQGKVTAVGEGTTEIVVTTEDGNFTAKCTLNVTTAD
Seq ID 218
MFLGLIFILFGVFGLFRLGFLGTLLANCLRLVVGNTFPFAAILLILYGLLVMIYGKDFPLKRGRPIFGAVLIYISVLLFFH
AFMFRNVSGSQPDILGNTWEFLQSDLKANQVTQNVGGGMIGALLYQGTYFLVAQFGSYLIATLLLLAGIFLMSMWDFQQIV
DHFQSIQDRLSHVSAKSQARQEEKEAKRAAKKEAKAAERQAKIEAAAQQKLQERERMEQAAAERLTKTPVETHQPMVEEPA
APTPVQIDSFQQQNQAMPVPPIAATKPQREQEEEAADEAGVLEFEISEEAEDRDYQLPPTDLLDTIQATDQSGEYEKIEKN
IGVLEQTFKSFGVDAKVVKASLGPSVTKFEVQPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNSAIST
VSFRDIVEAQPSHPDKLLEVPLGRDISGMVQTADLSKMPHLLIAGSTGSGKSVAINGIITGILMQAKPHEVKLMMIDPKMV
ELNVYNGIPHLLTPVVTNPRKAAQALQKVVQEMEFRYEKFAATGVRNITGYNQLIQQKNAEDGENRPILPFIVVIVDELAD
LMMVASNEVEDAIIRLAQMARAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDTNGAEKLLGRGDMLF
LPMGENKPIRIQGAFISDQEVERVVAFVTDQQEAEYQESMMPTDEPTTSGGGEAPQDELFEEAKNLVVEMQTASISLLQRR
FRIGYNRAARLVDELEAHGVIGPSEGSKPRKVFLQAESEEAATETPEQ
Seq ID 219
MRKSFNLAVQALTVQYQGRTALNNIHVTIPSGKITGIIGPNGAGKSTFIKGLLGLIKTKERDVLLNNQAIDQQKTTIAYVE
QRSALDLSFPISVFETVLLGTYPNLGLLKRPGKKEKQAAMAALKMVQLEDYAQRQIGELSGGQLQRVFIARVLAQGAEVIF
LDEPFVGIDMSSEKVIMDILKSLKNQGKMIIIVHHDLHKVSHYFDELIVLKNRLIAAGPVEQTFTAETLQEAYGDLLGDLL
IQGVAK
Seq ID 220
MIGLELTFTVPQADEVIRSLVETYHDEKDIVIGAGTVLDAVTARLAIMAGAEFIVSPSFNRETAEICNLYQVPYLPGCMTI
TEMQTALKSGVDIVKLFPGSTYGPSIISAFQAPLPYLNIMPTGGVSLDNMASWFEAGVTAVGVGGNLLAHAKTGEFGKVTA
LAKQYMAKFREIKEN
Seq ID 221
LAGVTKEQIAKAKEWDLLSYLQAYEPQELKKSGPREYCTRTHDSLKISNGKWCWNSRGIGGRTALDYLIKVRGMDFVGAVE
TLCGYCAPPPEKAPPIKEKQPKPFRLPQASRCASAVVGYLQDRGIDPELLGVCMEAGILYESCKYQNCVFVGRDTAGNARA
ASLRGTRDGFRMDVEGSDKRYSFCLPAGKADCPRLAVAESPIDALSLATLVKLSGGEPRDSHYLSLGGTGPRALIQFLHDH
PHVTQVSLCLDNDKAGLEGMERLTQEIQNDAELSRRVKLVYPNPPKQGKDYNEFLCTHVKAVRTAQRQRDAAR
Seq ID 222
VRIKEGRTVGLFTKVSPEEKAAIDRKMELLGTSNLRAYLRKMAVDGYIVQLDMGAVLELVKLLRSVSSNVNQIARRCNSTH
NLYEQDVEDLRQGYSEIWQGVNDLLKKFEAL
Seq ID 223
MANKLQTVSQLADQTAKAVTRNAEGWKSYLTTASRLYKYSFDEQLLIYAQRPDATACASMELWNEDMRRWVKAGSKGIALI
KKDGIRPRLTYVFDVADTRPVRGAKMPYLWEMKDGHHAAVMDTLARRYGETEKKDIGSALMEQAKHAVEEVYREHLSDLAY
DAQDSLLEGLDDFNLEVRFRNLLTASVQYTLLSRCGLNPADYLEDEDFDGIHEFSTPAVLHHLGSAASEVSMNMLLEVKKA
IRQAEKEKAQNRQKIPKNPEKPLAKEPVIGYTKVKEQFNTLKRESEERSIQNGRTGIQEDGRLPDSRLGDGRGGRDGGNAA
GQVRQAAADLSSGTPQGDIHLDAADRAAGTPPAGDRPAGAGTGRPDRGGIKETERRGRGDESPRPDGMGAGSQPVSRPGGG
NRTERDRLQVNQENQQEAAGEQSAVSASEKPAFTQFSLFPTVEQQIETIAQAQKTEQELRPAVSSGKVTDAVIGRALTSGG
NEPDSILRIVAFFQKDPTEQSAADFLQKEYGSGGKGLKIAGHEYSLWFSHSGIHIAPGRSAGGGSLVTWKQAAAQIRQLLN
SGQFASQDKIDAARDNEFRELSEKLWFLRQNFSEEAKEKGLLPTIDALYGGFPDSTAKIAALLKDPAERAKIAQEVRQFAI
SHQENPNLLRFRQRPAPSELFTRLTDMDKPVTAFHGVEGFAPARGAFVTEDEITRMFTGGSGVSEGKFRIYAYFMQGHDAK
ECIEFLKNEYGIGGHGYIGYDEWHDGKGIKLSRADDFSGGNYDTVTLNWKQVQKRIAGLIKTDRYLNRREKAYLPEYEKMQ
LARSLYTFQYYDPNDASKTIPHDWDVDAAKKVFRPLLDDPEQCAAHYEKMVKALAMVSPDERAYSLMEPTLQKMGAYLRGE
YSLFTPLPDAVLQEERQKKQKQKQKERQDSARQTAEPATGLAAAAKALSRKKQPAPREDADGQLTLDMFGLSSEPEQPAAT
PEPEPELSQAETGASETAVPIASKPEPPQETPNIPEGAAPIMAESPADRYDLGYGHMGNGLTVWNRLEEEHGDYKTVAHIA
PDRTVTFYDADMPEEIREKIQKVAAATEMSISATQDTPVFSTPPQEPERVQPGNSEPEPEKVQDAPAAVNPEPETGDSNIV
PSPAQKAGAEPTGTGSPQTQEAPKTAAADGLNLTPNVEEYLNLKAQYPDKLVGVRVGKTMLFYGTDAEEAAPALGTKTITR
DIPDLGMVSITGANGWQSVLKKLLEHGKSVVLARPDTERGGDAPYEIIKERSAADYIPVGMELTIDGRLMKIDSVDYNAGT
VSLQDMELRGWYPIFRSESIPFVRQFVEEVQQEHFTAEPMQEPERPETSDLDTAKQLIEQFAYAEYGSDDVDFSDLEHIGI
AYGTTEDGGLEVQVDVNLLDFSISQSVDGKCVETRQYGSLRELIDMELAFLDYDMLVSVEPDIEERLKAELNQRIRWSEMA
GAREGVEPTEPEIFPEKDVSPEQEAPPELVEIDGGQITETPAQRQTRRRAQEEVDSRVFPSEIILQPLRLEPERHNFRITD
ENLGAGGEKTKYQYNVEAIRTLKQIEAENRLATPEEQAILSRYVGWGGISHAFEPNDPKWAKEYAELKELLTPGEYQSAQS
TVLNAHYTSPTVIQAIYNAVEQMDFTPGTVLEPSMGIGNFFGMLPEKLAAAKLYGVELDDLTGRIARQLYQKADITVDGFE
RTDHPDDFFDLAVGNVPFGSYQVHDKRYDRRNLMIHDYFITKTLDKVRPGGIVAFITTKGTMDKKNSKAREALAQKADLLG
AVRLPSNAFKANAGTEVTTDILFFQKRDRIPEKLPEWVEVGQTEDGIPLNRYFLDHPEMVLGTMTMGRSMYGNETETACQP
IPGADLSGQLAEAIRHIAPPDRELLEVDSGQDGEELESIPADPTVRNFSYTLSNDKLYFRENSRMTQAVLGKTPTERVRGM
IGIRDSARRLIDLQLAGADDTEIQSEQAKLNRLYDAFSAKYGLLNSTGNKLAFEQDSSYPLLCSLEIINEEGKLERKADMF
TRRTIQTHRAVTSVDTAVEALAVSIGEKACVDLGYMASLMGGPEKIPQIVEDLKGIIFKNPATGPFDLEDGGAHWHQGWQT
ADEYLSGNVRAKLAIARAAAEENPQFAINAEKLEQVQPKDLTASEISVRIGASWIDPEYYQQFMFELLHTPVYLQDRKIKL
QYSPTTGEWNVQGKSTDNRDNVRVYATYGTKRINAYEIFEQTLNQRDVRIFDKTEVDGKEVRVLNEKQTAIAQQKQEAMCE
TFKDWIFKDPERRETLCRRYNEKFNCIRPREYDGSHIRFAGMNPEISLRTHQENAVARMLYGKNSLLAHCVGAGKTFEMIA
AAMEGKRLGLNQKSLFVVPNHLTEQWGGDFLRLYPGAKVLVATKRDFEPARRKKFCARIATGDYDAIIIGHSQFKEIPLSP
ERQKAVIKGQIDEIVTAIAEAKAEDGERYTIKQMEKTKKNLEAKLQKLADGKKKDSVVTFEELGVDRLFVDEAHGFKNLFL
HTKMRNVAGIAQTDAQKSSDMFAKCRYMDEITGGRGIVFATGTPVSNSNVELYTMMRYLQFDTLEQNGHRHFDAWAADFGE
KVTAMELKPEGSGFRSKTRFAKFYNLPELISIWKEAADIQTADMLNLPVPKAEYITVTTEPSGFQKEMVEELGERAESVRG
GQVDPHIDNMLRITSDGRKLALDQRLQNPLLPDDPDSKVNACVKNIVQEWQASTEILGTQLVFCDLSTPKGDGSFNVYDDI
KEKLIAKGIPEAEIAFIHDANTETQKAELFAKVRRGQVRVLIGSTAKMGAGTNVQNRIVASHDLDCPWRPADLEQRAGRSL
RQGNMNASVKMFKYVTKGTFDAYNWGLVENKQKFIGQIMTSKSPARSAEDVDATALSYAEVKALATGDDRIREKMDLDVQV
SKLKMLKANHTSQQYEMQDKALKYYPQKIAETKLLIEALTADLPTVQAHPVKDDAFSMTVMGQTYTERKAAGEAVIKACML
MDDPEKTVDLGEFRGFPMQLRCDGSKFRVTMKQNLTYSAELSDDAVGNVTRINNALESLTERLDAQKARLATLESELENAK
EEADRPFPKEEELREKSARLNQLNRELSRPDKKEDEQEVEESPDLEDAGKPAPVVPMPAVAAGYKPSIRQAIRNYEPPTPV
HSGMERTPRKEAVL
Seq ID 224
MPVLTSGQQERKSKQRKIRNSEYYDMEGTFDRLYAESKKDKTFNHLMEIIESEENIKLAYRTIKKNTGSDTSGVDKRTIAD
LAKLSEEEYVRLIRKQFSNYHPGPVRRVEIPKPNGKTRPLGIPTIVDRIVQQCILQVMEPICEAKFSENSNGYRPNRSAET
AIAQCMRLIQVQHLYHVVDLDIKGFFDNISHTKLIRQINALGIRDKKLLCIIKEMLKAPVVLPNGEKTYPARGTPQGGILS
PLLANIVLNELDWWIASQWEEMPTKTKFKTRSNAQGTEIKSHAYRALRRSRLKEMHAVRYADDFKIFCATHEDAVRAYKAT
ELWLKDRLGLEISPDKSKVVNLKRQYSDFLGFKLKVRKKGKKYVVRSHMSDKAYKKAHEKVSEEVKKLAYSSDDNAQFMQL
QKYNSVVAGLHEYYCISTEVSHDFSRLAFSINKQLRNRLKGDLSKKGQLRNGFIKEKYGASRQMRFLHGRPVVPLGYVQSK
NAQHKRKSINKYTVKGREQIHKNLAIDTATMLWLMRNPVKGRTIEYADNRISLYAAQYGKCAVTGIPMNSHDIHCHHKVPV
SNGGSDEYANLILVSKAVHILIHASSEPTIEKYLKSLNLDNKQIEKLNKLRSMAEMPPIIL
Seq ID 225
VTEKARDLFDKLMTQIQLDDTAKEHPLIQNGRIDKVIVHQQSRLWEFHLSFDELMPVMLYQTFMQQLELAFQQIAQVSVQI
TTNQTTFTEEQLTDYWQLALLNSQCNTPLVQKVLKTQTPQFEDRKIILPVDNEAVIPYLKQQYLPIIEELYFSYGFPKFHI
EPKMDQQQAAEVLKKFEEQKLEQAAAFQQQAAESLVKHEQMKKEKQQQAPAFDGPIRLGRNIPNDEPIMPMGNILEEERRI
TIEGFIFDKEVRELRSKRKILILKITDYTSSFVVKKFSNGEKDEQVFDAIQPQSWIRVRGSVQEDTFMRDLVMNAQDLMEV
AHAPRKDYAPEGEKRVELHMHSNMSTMDATNKVGDLVAQAGKWGHKAIAITDHGGAQAFPDAHAAGKKAGVKILYGVEANI
VDDGVPIAYNEEHIELTDATVVVFDVETTGLSAVVDTIIELAAVKMHKGNVIETFEQFIDPGHPLSQTTINLTGITDEMVR
GSKSEEEVLRMFKEFSEGTILVAHNASFDMGFLNTSYGKHGIPEAANPVIDTLELSRFLYPHFKSHRLNTLSKKFGVNLEQ
HHRAIYDSESTGHLCWIFLKEAKENHDMHFHDDLNRHIGEGDSYKRARPFHATILATTQAGLKNLFKLISMSNVDYFFRVP
RIPRSQLSKLREGLLIGSACSNGEIFEAMMQKGVEEAKNRAKFYDYIEVMPKPVYAPLIEQELVKNEADLEEIISNLVKIG
DELGKLVVATGNVHYLNEEDAIYRKILVGSMGGANPLNRHSLPKVHFRTTDEMLTEFQFLGQDIAKRIVVENPNKVADLCE
EVIPVKDDLYTPKIPGSEQEITDLSYNRARELYGDPLPEIVEKRLEKELNSINGNGFSVIYLISQKLVHKSNEDGYLVGSR
GSVGSSFVATMTGITEVNPLAPHYYCPECQYSEFYEDGSYGSGFDMPEKACPKCGARLFKDGHDIPFETFLGFHGDKVPDI
DLNFSGDYQAEAHNYTKVLFGEEYVYRAGTIGTVADKTAYGFVKGYERDHNLHLRGAEIDRLAKGSTGVKRTTGQHPGGII
VIPDYMDVYDFTPIQYPADDQEAEWKTTHFDFHSIHDNILKLDILGHDDPTVIRMLQDLSGIEPKTIPTDDPEVMRIFEGP
DVLGVDAEQIYSKTGTLGIPEFGTRFVRGMLEQTHPSTFAELLQISGLSHGTDVWLGNAEELIRRGEANLAEVIGCRDDIM
VYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRDTYLNAMKENNVPDWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVY
FPILYYCAFFSVRADDFDLVAMSQGKEAVKARMKEITDKGMDASTKEKNLLTVLELCNEMLERGYKFGMIDLYKSDAENFV
IEGDTLIAPFRAVPSLGLNVAKAIVAAREEQPFLSKEDLATRGKVSKTLIEYMNENGVLKDLPDENQLSLFDML
Seq ID 226
MERSNRNKKSSKKPLILGVSALVLIAAAGGGYYAYSQWQAKQELAEAKKTATTFLNVLSKQEFDKLPSVVQEASLKKNGYD
TKSVVEKYQAIYSGIQAEGVKASDVQVKKAKDKQYTFTYKLSMSTPLGEMKDLSYQSSIAKKGDTYQIAWKPSLIFPDMSG
NDKISIQVDNAKRGEIVDRNGSGLAINKVFDEVGVVPGKLGSGAEKTANIKAFSDKFGVSVDEINQKLSQGWVQADSFVPI
TVASEPVTELPTGAATKDTESRYYPLGEAAAQLIGYTGTTTAEDIEKNPELSSTGVIGKTGLERAFDKELRGQDGGSLVIL
DDKENVKKALQTKEKKDGQTIKLTIDSGVQQQAFAIFDKRPGSAVITDPQKGDLLATVSSPSYDPNKMANGISQKEYDAYN
NNKDLPFTARFATGYAPGSTFKTITGAIGLDAGTLKPDEELEINGLKWQKDKSWGGYFATRVKEASPVNLRTALVNSDNIY
FAQQTLRMGEDKFRAGLNKFIFGEELDLPIAMTPAQISNEDKFNSEILLADTGYGQGQLLISPIQQATMYSVFQNNGTLVY
PKLVLDKETKKKDNVISANAANTIATDLLGSVEDPSGYVYNMYNPNFSLAAKTGTAEIKDKQDTDGKENSFLLTLDRSNNK
FLTMIMVENSGENGSATDISKPLIDYLEATIK
Seq ID 227
MKKVLMGVLSLGLLLGAATGCTSDQEKAAGKTKASSEKTEATSGASANGYTDPSELKDSYDVVIVGSGGAGMTAALQAKEA
GMNPVILEKMPVAGGNTIKSSSGMNASQTKFQEKEGIKDSNDKFFEETLKGGKGTNDQELLRYFVDHSAEAIDWLDTKGIT
LSNLTITGGMSEKRTHRPADGSAIGGYLVDGLVRNVREEKIPLFVDADVTDLVEENGQIDGVKVKMKDDKEKTVKAKAVVV
TTGGFGANEKLITQYKPELKNYVTTNQEGTTGDGIQMIQKVGGALVDMKEIQIHPTVQQSDAFLIGEAVRGEGAILASQKG
ERFVNELDTRDKVSAAINALPEKSAYLVFDQGVRDRAKAIDFYDQKGFVEKGETIEELAEKIGMPADTLKATIDIWNQDVN
AKDDKQFGRTTGMEADLSTAPYYAIKIAPGIHHTMGGVKINTKTEVLREDGTPIKGLYAAGELTGGLHGQNRIGGNAIADI
IIYGRQAGTQSAEFASAQK
Seq ID 228
VIVRGGGDLATGVIQKLWHVGFKILVLETECPLAIRRTVSVCDAIFQKEQRVEDLVAVRITSLKDCAKCWQQNKLPVFVDQ
TASAIQQLKPLIVIDAILAKKNLGTHRGMAPITIALGPGFSAPQDVDVVIETMRGHRLGRLYFEGTALPNTGIPGEIGGKS
AERVVHAPASGQVTHLKNIGDLVLKGEALFLIDQVPVYSPLTGTLRGLISEKVTCYQGLKCADVDPRPVEKVDCLTISDKA
RALGGAVLEAIFMIGRRKNVL
Seq ID 229
MYSIIVNGQSETCETNKKLMDFLREDLGLTGTKDGCNQGSCGACTVLVNGKASKACLFTLEKLAGKEVTTIEGLSQRQKDV
YAYAFAKTGAVQCGYCIPGMVISAQGLLNKKPEPTKEDIQKAIRGNICRCTGYVKIIEAIQLAAKMFCEEASIPEEHSNGK
LGEDFQRVDAVEKTLGTGIYVDDIDIEGMLHASALRSAYPRAKVLSIDSTQALAHPDCVAVFTAKDVPGNNKIGHLEFISD
WDVMIPEGEITRYVGDAVALVVSKRKETLPEIKNLVEVDYEEMIPLTSCEAALAEGAPAIHEKGNILSHEHLVRGNAEEVL
ENSAFVVTEHYSVPINEHAFMEPECAIAQPEGEGILLFSAGQSIYDEQREVARMLGLDKEKIHVQSKLVGGGFGGKEDMSV
QHHASLAAWLLKKPVKVLLSREESLMVHPKRHGMEMDFTTGCDEEGNLTAMKAVIYADTGAYASLGGPVLQRACTHAAGPY
KYQTIDVEGFAVTNNPPAGAFRGFGVCQTAFAIESNLNLLAEKVGLSPWEIRFKNAVAPGDTLPNGQLVSKNAALDKEALL
AVKDVYEQAEVAGISSFFKNSGVGVGLPDTGRCIISVEEGKIHVRTSAACIGQGMATVTTQIACETLNLPPEMIVAEAPDT
RRTPNSGTTTASRQSLFTGEATRRAAMQLRYELDMGRALSDLEGEEFYGEYSAKTDPLINDKKSPVSHAGYGYAAEVAILD
DKGKVAKFVAAYDMGQVVNPRAAEGQIEGGIAMGMGYALTEKFALEEGYVKAKYATLGLVNATQVPPIETILVHADNLHEG
LAYGIKGVGKLATIPTAPALAGAYFALDGQLRPSLPLENTPYQKKKR
Seq ID 230
MSDIMHPISIEALLNWIFSEYQQDGTIFGIRKFYHADPTKTISLFGEKMETPCGPAAGPHTQLAQNIIAAYLTGSRFFEVK
TVQILDGEDLPVSKPCIAAADECYNVEWSTELRVPQAYDEYVKAWFVLKLLSKEFELGDPNGFIFNMSVGYDLAGIQSPKI
DRYINEMQNAEGTPIWAECQAAAKKYLSYFKKVDDLYIEAISPKVCHSITLSTLHGCPSDEIERIAAYLLSEKGLHSFIKC
NPTMLGYEYARQTMDELGFDYMVFDDHHFKEDLQFEEAVPMLQRLQLLANSKNLSFGVKITNTFPVTIAANELPGDEMYMS
GRSLFPLSISLAQKLSEAFDGKLQISYSGGADIFNSKEIFDAGIWPITMATTLLKPGGYQRMNQVANVLSAAEYPQMVHVN
LDKLAQVVEKAKTQARYQKSIKLPESTKLPKTVPLTDCYIAPCRSDGGCPINQDIPAYLRYVSEGNYLKALQVIVDKNPLP
FITGTICAHPCMTKCTRQFYEESIHIREVKLEAAEHAYDELLTTLEKPQPKENAPKTAVVGGGPAGISAGYLLAREGMPVT
VFEKSETIGGVCSQIVPEFRISMESVQKDVQLAEFMGAEFRTGQEAPSLAELKNQGYTNVIYAIGAWKHGVLRLESGRALN
SLEFLKANRENPTINPYGEQIVVVGGGNTAMDCARAATTLPGVKKVSVVYRRNKRNMPADEEELYLALEDGVDFLELLSPI
KHENQQLTCEKMVLGERDASGRRRPIGTGEMIDIPADTVIAAVGEKVDTEFYQALGIHTDNYGKVVSNQETLETNIPGVYV
IGDANLGPATIVEAIADATKAANNICLVHNHHYEKDNLNSDVAFVRNKRGILVTDEMSCSQASRCLECSTICESCMDVCPN
RANIVVYVEGKPQIVHVDRMCNECGNCETFCPYASAPYKDKFTLFNSEADFYDSTNSGFYVQNPVDKICLLRLWGTVSTIQ
LTDQGLDVPEDLIALMVTMIEEYAYCMQA
Seq ID 231
MTKQTIKEQILHFMESQKKKSFSMEEIAQGLNLEKSSDFKILVQTIAQMEREKSVSFNKKGKVLLPMKDLLIEGTFRANER
GFGFVTIDPEEPDVYIPKEATNFAMDGDTVLIDVIQHADPFSDRGAEGKVKEIKERAVSQVVGEFVAYSEEEMAEMGLYGY
MIPKDKKLNQYTVSIAPEGIKPVDGSIVIAEITYYPDQEYPTSMEGLVKQVIGHKNDPGMDILSIVVAHGIPTAFPDEVLA
EADQVPETIAESDLVGRRDLRDQLIVTIDGEDAKDLDDAVTVQKLANGNFFLGVHIADVSYYVTEGSQLDMEAYERGTSVY
LTDRVVPMIPQRLSNGICSLNPHVPRLTMSCEMEITPEGEVISHEIFQSVIQTTERMTYTAVNEILEEQKPETLERYKELV
PMFKEMGELHHILEEMRMRRGAISFEDREAKVLVDENGHPKDILLRTRGVGERLIESFMLAANETVARHYHDLKLPFIYRI
HEQPKEEKMQRFFDFAAVLGILVKGTKENISPKDLQKVLEQVENKPEEVVINTMLLRSMQQAKYSEDNYGHYGLAAEYYTH
FTSPIRRYPDLIHRLIRRSYSQDQSEKNQEKWNEALPEIANHSSSMERRAVDAEREVDAMKKAEFMVDKVGETYDGIISSV
TKFGIFVELPNTIEGLIHVNNLNQDYFHFIENHMALVGERTGMTLKIGQKVQIRVEKADPETREVDFELISAEEVAPVEGP
KGRKKGKANSSTRSNNQRRNKKDESFDGKKKKNKKKGKGKKQPFYKEAMKQKNKKGKKKK
Seq ID 232
MKNRYFILMILSFYFFAFGIETSAAELRFSVETEIPENQIDKTKTYFDLMMKPDQEQILKVRAANSTDENLVIDVSVKSAT
TNSNGVIEYGESLTALDKSAPADLSEIIQLKDGGESVELPAKSEKAVELRVKMPKEEFSGQLAGGITFSEKVDETKDKQKE
NTNGLAIENRYAYTVAVLLRENETVVQPELSLEKVEPTQRNARSVISATLLNHEAAYLQSMKVTANVKNKKTNNVILEKEQ
EDMQMAPNSIFNFPIPYEENEMEAGTYVLAMTVEGSGKKWQFTKEFTISKEEAKTFNEKDVTVKKTESKLIYLLIGLLILL
LIICLFIILRLKKQKNK
Seq ID 233
MSRKRKISLISLVIILVFVTVGSAYFAVAGSYLKKTIDKGYVPIKNDYNEAQNKDSQSFLIMGLDNTIERKLGTTRTDAMM
VITVNNKTKKITYLSLPRDSFVQIDAKNYQGMQRIEAAYTYDGPTASVNTVEKLLNIPINHYVVFNFLSFIKLIDAVGGID
VNVKQAFDGVTKDGPGSIHFDAGKQHLDGTKALSYARERHSDNDIMRGFRQQEIIQAVEDKLKSGQSIMKIMDIIDSLNGN
IQTDVDSNELTHLVKEGLTWTNYDKQQLSFDWRTFSNEGRSMVELYPDSIENVRHQLRVSLNLEKPDERDQDGYVFHTNGE
FLYQSDYTVQDEAAEENEMTSINGNTYIGVPGNTQTGPLPSVKTENGFIK
Seq ID 234
MKKSQAVQLIQELSNANGVSGFETEVVRILQHATADFTIQRLDSIKNLYLEKKNNLSEGPVVLFDAHSDEVGFMIQAIKEN
GLLRFFPLGGWVPNTISAQKVRIRNREGTYLPGVVTSRPPHFMTPEERQRPLTIADLTIDIGATSKEEVIETYKIDLGAPV
IPDVTCCYNEQTDLFLGKAFDCRIGCACLVDVMEELKEETLPFKLVATVTAQEEVGERGALIAAKQVNPDLAIVFEGCPAD
DTAETPEMIQSAMGKGPMLRYFDVSMITNPEFQEYALEIAKIHKIPVQVSVRSGGGTNGMAITQVQGAPTIVVGIPVRYAH
TPHCYVDFQDYQAAKELVIQLIKNLDADKIQALVQPLSKEWNK
Seq ID 235
MTTSQMIADFTTLAIQAGGWMELDRLYLQNRLLSMIGEQELGEVDIRPVATPAADLAEQLCQVASANQLVKTEQQKEQFMV
QLMDLLTPPPSVVNAFFAQHYAKEPQEATEYFYQLCQKNGTVIEQEEPVVFSTVYGDFLANKVHSEASKATLSAQSYPRCE
WCMATEGYQGSQQFPATTNHRVIRMNLDGESWGFSFVKQAQYQQQGVIAFEkHQSAKRSIKTFQQLLKIVEVFPHYFAGID
ADFEQNEHVYYQTGLQQFPLAEASISEYVELANYPLINAGMVNWPVETFRLEGPNASEVAQAANDIFEQWQMLKLPTDEIQ
IVARRKELLYVMDLIFSRPQAKPSLTLAEVQGLTTWNNQKTQALETVASAYQQRLKEASAFAETSEGKAAFLAMVAPVTH
Seq ID 236
MSILWVINGILLVIVLAMVFNELYLKIMVKRSAKMLTEEEFKETMRKAQVIDVREKDTFDAGHILGARSMPYSMLKTTIGS
LRKDQPVYLYDQKKALSIRAANLLRKNGYTDIYILKGGYDGWTGKVKKRNS
Seq ID 237
VIFMNPDEPNFIWKDLNAVRDMGCIIENELSEVLPNKRYETYSIIGRSGEFNETFNDYEPFDYEIEDVTIPYENLAAVKRW
LTGKSKLITHNDEDKYLDAICTMSKPTSFKNEWGVFYTFNVEFRCQPFKRKVNEQPKVIKTKSIEITDHGDEIAFPYIEIN
SKGGDITLNIGSNSLTILRTQSGIVTIDTEKGKAIQEGNPLFTRGSWIKTNPGQNKLNISGNFIEAKFWNRSAYL
Seq ID 238
MSIHITFPDGAVKPFDSGITTFDVAKSISNSLAKKALAGKFNGVLIDLDRPIVEDGSLEIVTPDHEDALGILRHSSAHLMA
NALRRLFPNIKFGVGPAIDSGFYYDTDNGESPVTAEDLPAIEAEMMKIVKENNPIVRKEISRAEALELFADDPYKVELITD
LPEDEIITVYDQGDFVDLCRGVHVPSTGRIQVFKLLSVAGAYWRGNSDNHMMQRIYGTAFFDKKDLKEFIKMFEEAKERDH
RKLGKELDLFMVSQEVGSGLPFWLPKGATIRRTIERYIVDKEISLGYQHVYTPINADVELYKTSGHWDHYHEDMFPPMDMG
DGEMLVLRPMNCPHHMMVYKNDIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPDQIKDEFKRTLEL
MVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAMDELELDYFEAEGEAAFYGPKLDVQVKTALGMEETL
STIQLDFLLPERFDLTYVGEDGENTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVEAHSEYAYEIK
ERLQAQGLRVEVDDRNEKMGYKIRASQTQKVPYQLVVGDKEMEDATVNVRRYGSKETSVEDLSIFIDSMAAEVHNYSR
Seq ID 239
VSNKKVAGTIILNLNDGSKKFLMHPIGEAIEFAMAKVSDEMTGLASMLQWFKEEVQLDVTSISLVELTNAHINKENVPLFV
FEMDEQALKNEMDKDYTWVAPAELKTIWSKYHIEGVPMF
Seq ID 240
MKKFDGVIFDMDGLLFDTELIYYTSTQKVADAMGLPYSKEVYLDYVGISDEEVQENYRRIYASYGHDTVEEFIRRSYDDTL
QEFRSGNVPLKPGVVEFLDFLDDQKIPRLVASSNVRPAIEMLLSHAGIQDRFVGIVSAEDVKRAKPDPEIFQKARQLLGTE
APKTLIFEDSFHGVSAAHSAGIPVIMVPDLLQPTEVIQEKTLHVLESLHQAPHYLK
Seq ID 241
MNELVFLHSQYIEEEPYTTDEVIAKYSQIKRESVSKLIKKYQKDLEEFGKVGFEIRAMESGQKAKFYQLNEEQATLLITYL
DNTEPVRRFKKALVRQFYDMKNGLYARRMERQKEKSVRKSMTDVIKELGLSPHYYKHYTDLVYKTALGFNAKQLREAREVS
KKSTILDFLTSHEIEAVNKREQQVATLLTLKMDYDTIKSILNGQGVLYQTTLKMPVATN
Seq ID 242
MPKPGETIHAIEHFTAGGGKGANQAVAAKRSGAETYFIGAVGNDGAGAMMTDLMSQDEINLTGVTTLEKTATGQAFIMVDN
AGENSIMIYAGANNAFTPKQVQEHQEIIEKSDFVIAQFESAIDSTIAAFKIAKKAGVKTILNPAPALEQVPEELLNVTDMI
VPNETETEILTGIKITDEASMRKAAEALHQLGIEAVIITVGSKGAFYDVNGRSGIVPAFKVKAVDTTAAGDTFIGALSSIL
EKDFSNLEEAIRYGNKASSLTVQRFGAQPSIPYQHELADK
Seq ID 243
MIKLAHIHKYYYSEEETLHVLDDINLQVDAGEFLAIMGPSGSGKSTLINLLGFIDKKFEGTYLFEDREIGDFSDKELSRIR
NEAVGFVFQNFSLIETLTVEENIELPLLYSGLTPKEAKDRVHEVLTKVGLPDKGKKHPKQLSGGQQQRVAIARAIVNRPSF
IIADEPTGALDSKTSEEILTLFQQLNNEGVTIILVTHDEETIEYCNRLIKVRDGKILEEVLT
Seq ID 244
MKKSGQKKRNKKVWFGIGAAVVVVGFIGAKTVFSSKEVEPEYTTYTITEMASLKLDGQVSFLDTRDIFFDPSLGKIAEINV
ENGKEVKKDSPLLTYNNSDIQATETEQANAVNRNNLQVQQAQENVNLATQKYNEALNKVAAAKQKLNTAKEAEEKETLNAE
IQQLNEAVSAANSEVAQANQALQLANSDAVGAANTLEQTRGKVNTVVTAPIDGQVTVDASAMSSTDKPVIKIATQKKNIQG
KVTEYDYDKLQTGEEVTVTTVGSGKSAPGKIVSIAQTPIAKNEGNPVVSYQFTVEGDFPWAEGLSTSIAVPQKQMIIPTAA
IQKEGQKEFVYVYKAGKAKKTPIETETNLGRKVVKSGLNWKDQVIANPNKELKDNQDVQVAAND
Seq ID 245
MIKKIIVVVAFMLTGFSLTAMSASAEEITDLFLQKEVTYSGVEGGKIGENWKYPQPVGEKAVDGDETTRWSADKQDEQWLI
VDLGEVKNIGELVLQLHAESPVYEILVSTDGESYQSIFKEENGKGGQPTKKYIDGNNVQARFVKYQQMKMWQHTNKQFYSS
SIISFEAYEKKRLPEAIKLLTENLTISEKRKQQLAFEVSPAGVDITEDQIEWSSSDPTIVTVDQTGNLTAVKSGEAKVTVK
IKGTEISDTIPVTVVAENKQYAEMRAKWKMRLLGTTQYDNDADVQQYRAQIATESLALWQTLNQAADRNYLWERKPSDTVS
ADYTTQFTNIKKLALGYYEPSSELFEKPEVYDAIVKGIEFMIDTKKYNGTYYTGNWWDWQIGSAQPLTDTLILLHDDLLNT
DAEKLNKFTAPLMLYAKDPNIQWPIYRATGANLTDISITVLGTGLLLRDNQRLVQVQEAVPSVLKSVSSGDGLYPDGSLIQ
HGYFPYNGSYGNELLKGFGRIQTILQGSDWEMNDPNISNLFNVVDKGYLQLMVNGKMPSMVSGRSISRAPETNPPTTEFES
GKETIANLTLIAKFAPENLRNDIYTSIQTWLQQSGSYYHFFKKPRDFEALIDLKNVVNSASPAQATPMQSLNVYGSMDRVL
QKNNEYAVGISMYSQRVGNYEFGNTENKKGWHTADGMLYLYNQDFAQFDEGYWATIDPYRLPGTTVDTRELANGAYTGKRS
PQSWVGGSNNGQVASIGMFLDKSNEGMNLVAKKSWFLLDGQIINLGSGITGTTDASIETILDNRMIHPQEVKLNQGSDKDN
SWISLSAANPLNNIGYVFPNSMNTLDVQIEERSGRYGDINEYFVNDKTYTNTFAKISKNYGKTVENGTYEYLTVVGKTNEE
IAALSKNKGYTVLENTANLQAIEAGNYVMMNTWNNDQEIAGLYAYDPMSVISEKIDNGVYRLTLANPLQNNASVSIEFDKG
ILEVVAADPEISVDQNIITLNSAGLNGSSRSIIVKTTREVTKEALEKLIQEQKEHQEKDYTASSWKVYSEALKQAQTVADQ
TTATQAEVDQAETELRSAVKQLVKVPTKEVDKTNLLKIIKENEKHQEKDYTASSWKVYSEALKQAQTVADQTTATQAEVDQ
AEAKLRSAVKQLVKVPTKEVDKTNLLKIIKENEKHQEKDYTASSWKVYSEALKQAQTVADQTTATQAEVDQAETELRSAVK
QLVKVPTKEVDKTNLLKIIKENEKHQEKDYTASSWKVYSEALKQAQTVADQTTATQAEVDQAEAKLRSAVKRLTLKNSGEN
KKEQKNGGNNGHLNTSTGVDQTGTKQVKPSSQGGFRKASQFLPSTGEKKSIALVIIGLLVIASGCLLVFRKSKSKK
Seq ID 246
MKKSVLFTSLLVLSSLALAACGGGSDDKGASNGGSDNQVYTMVESQEMPSADPSLATDEVSFTTLNNVYEGIYRLDKDNKP
APAGAAEKATVSEDGLVYKVKLREESKNSDGKPVTAADYVYGWQRTVDPATASEYAYMFEPVKNAEKISKGELPKEELGIK
AINDHELEITLETATPYFDDLLAFPSFLPQRQDIVERFGKDYTKSSDKAVYNGPFTLTEFDGPGTDTKWSLTKNEEYWDKE
TVKLDKVAINVVKEAPTALNLYETGEVDDTYLSGELAQQMQNSPDLVQLKAASSFYLEMNQADEKSPLTNANLRRAMSYAI
DRDSLAKNILANGSLPSQGFVPVDVAKSPKTGEDFVKEAGSDKLVKYDKKKAVEYWNKAKQELGVSNLTVDLMVDDSEGAK
KMGEYLQGSLSDTLEGLKVTVTPVPMAVRLDRTLKGDFQIAVRGWSADYSDPINFLDLLESSTSNNRGRYSNPEYDKFIAA
SKTTDVNDPEKRWEDLINAEKTVIADMGVVPIYQKAESHLRAPNVKEIIYHPTGAKYDFKWAYKE
Seq ID 247
MSKKPSGYQKKVLTVLLVSFGFGGLLAQFSPMKSGGFSSWFDDSYVKASAESSKTKEPAPVKIEKKVKPLSYGQQVNQEIE
KKQYDGHLDLPLELQTDAKWKDTAYGFGNVDKPNTIEINGCAIVSLAMVGSYMDHQEVTPLDVLAWAKNDFFMEGQGTAWS
IFSAYAEMKGYNCQEIGDIETVAAFLKEGHPVIISVKPGYFTTTGHIMVMSGVDEKGDFWINDPNDSEEKGHSKRTFTAEE
VMNEALNFWAFY
Seq ID 248
LKKSVLSALMVCSITLTSVALPSAAFADEYDTKIQQQDQKINALTSQMSDAEAKVAAIENDMVETAKQIDTLTAKKNKLSS
EVSKLYSEISDLNVRIQKREVQMTKQARDVQVNGQSDSIIDAVLDADSVADAIGRVQAVSTMMSANNELLEQQKEDKATVE
KKTKNVEKQIAELEAATKELNDKTESLKTLKIQQEVAKNDLEAQRSEEQGKKDGFIKQKKEAEKRLAEEQARQRAAAKKAE
EQAAAQAQAAAQKAAAEQAKATKAANEAAASAAEEKAATPVVESSTTTESTTTQETTTSSTETESVVTTPVAAAPEKEKEV
PVTNPTTPEKGNEAKPGNGGVTSGKQAAINAALADVGNSYATGWNQPGECLVSVRRWLAAGGINFGYGGPNSGYVASGATQ
VSWSNVQPGDVVQYESAYSPDSWIGGVHTVLVTGVSGGSVQIVEANNPGGSGYVSSNSNWSPAPPAGFRAVVWRFPG
Seq ID 249
MSSLLKRLVQLVLLVVAVLLIRHYVFSPAAVNGSSMEPTLHNNDRLWVTSIKKPQRFDIIAFPSPRNGQRVAKRLIGLPGE
TVEYRDDTLYINGVSLSEDYLASAKRNVSKNENYTQDFTLETLEATQSLTVPEGMYFVLGDCRPRSDDSRYFGFVKQASVE
GVLTFRYYPLDKIGFP
Seq ID 250
MANDDLQQRLDKGMYGTPLVNPEEQHKYMGTFRERCRLSMTVAEMKDAQNQKHLLEELAKHPEVTVLLNGEISSDLQSTYI
KLLNQHGANFKIVNNFVENNPDSLGLLLAEKHAVDEPVIDVTEKYPQATETPKEEPTAKKSFWQKLFHS
Seq ID 251
VYLKRIEITGFKSFADKTIIEFEDDVTAVVGPNGSGKSNITEAVRWVLGEQSAKNLRGGKMNDIIFAGSEGRKPLNIAEVT
VTLDNSDHYLALDYSEISVTRRLKRTGESDFFINKQACRLKDIQDLFMDSGLGKESFSIISQGKVEAIFNSKPEDRRGIFE
EAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIYELEDQLVPLAAQADAAKKYLALKEELTEIDVNLTVTEIQEAKAIWET
KTQELTAIEEKLAGASKQVHDLEGKLVRLRSKRNRLDEQIETEQQQLLQVTEALKQAEGQENVLIERSKHTSQTASEYEET
LAETAEKIVRYREELQTLETAIAEKTAQRQTLKEALALATKDVEKYSKSSKELMEELRSQYVEVMQEQANTANDLKYLERQ
YQQETAKNQQSLAKHEALEEQMVEALAMKETLEKEQKVAKQGLQEQLEEYTALKATLEAKRERLAQRQNDMYQAMNQVQQA
KARQKSLQEIQENYAGFYQGVKAVLRHKNQLTGIVGAVAELIEVPKEYTLAIETALGGAAQHIVVENEKDGRAGITFLKQQ
HSGRATFLPLTTIKPRSVSAMVQNRLAGAPGFVGIASELVRYPEQVQTVIQNLLGVTILAADLTSANQLAKLVNYQYRVVS
LEGDVMNPGGSMTGGANKRGNQGSLFSQAQELQTITEQMTQLETQLRSVEQEVQALSQEVKTATERAEMLRSAGEQNRLKQ
QEIDNKLANQTETITRLTKEKRLFEYESRELHQFLTEYQTKKATLTEQQANLTATKERLDAEMKQVEQEASQMETFKAQAQ
ERLTTVQAEQAVAAEQCAHFARQKQDKQEQLDELLIRETAIRQQLQQLSSHSSDHQLTEEGLAAQVAQLAEKQTALQTSLQ
TARSQRQALQEEVDELDTKLAEENKRQQQYLADKTQIEVLKNRAEMQLDSSLSYLQEEYSLTFEAAYEAYFPIDDLAQAQQ
TVKRLKQEIERLGPVNLSAIEQFEQVDERHQFLVSQRDDLLNAKEQLFETMDEMDQEVKERFKEVFEAIRGQFKVVFPNMF
GGGRAELVLTNPEDLLNTGIEIEAQPPGKKLQHLSLLSGGERALTAIALLFSIIRVRPVPFCILDEVEAALDEANVARFGH
YLSEFEDGTQFIVVTHRKGTMEAADVLYGVTMQESGVSKIVSVRLEEVKEGGAIEKSN
Seq ID 252
MQKVAKVIVDVPTMQTDQPFTYLVPENLNEQLAVGMRVEVPFGNGNRHVQGFVLAIEPVAATVLDETNVQLKELVAVLDLK
PVLNTEMLALADYMKEKTFAFKITCLQTMLPSVMPADYQKYIYLTDELSEELQDQLFYGLEEISWDQAQERGLLPQLMALR
KQQKVDIRYEVTTRNKVKMVRFIQAAKEFEQLEEIRLGLRKGAKKKEQLLYYLQRLGTEKVTAVKEMKELGFSTALLNEAA
NNGWLTFIEKEAYRDPFANQTFEKTTALSLNAEQQVAVETILQSVQEQQSQTYLLEGITGSGKTEVYLQVIAEVLNQGKTA
IMLVPEISLTPQMVQRFKSRFGEHVAVMHSGLSQGEKYDEWRKIERGEAEVVVGARSAIFAPIENIGVIIVDEEHEASYKQ
EETPRYHARDLAIWRSEYHHCPVVLGSATPSLESRARAQKNVYQRLRLTQRANQAATLPTIDVVDMRQEVENGNVSSFSMS
LQEELQERLEKNEQSVLLLNRRGYSSFVMCRDCGYVLPCPNCDISLTLHMDSKTMKCHYCGHEERIPYRCPNCGQDKIRYY
GTGTQKVEEELQTLLPDSRILRMDVDTTRRKGAHEKILRTFGEGQADIILGTQMIAKGLDFPNVTLVGVLNADTALNLPDF
RSSERTFQLLTQVSGRAGRAEKPGEVIIQSFNPEHYAIQLAKAQDYEDFYTKEMYIRHRGDYPPYYFTVQITASHPEENEA
AKQMFQIATKLKQGLSPQAILLGPTPNAIMRVNNRYFYQVIIKYKQEPMLQPLLKEILTDTQPATARGLKLSIDAEPMNFI
Seq ID 253
MDLHLTVDLKKTKTLIDLADQAHLIIEQTTKLPKKEAAILFLLIDNQLVALGLAEEKATYKEVSFDHSILLKPTWDTELAY
LAQAFLDDAKESGLTLEATPTTVKIPKSAVATVTNYKETVTFILERFGYSLFKKPAPKKARPAKARHKWTKEVSQIPFYID
TRQSKATVFWQKRNEMLIKAGATMMPEAPLNKDGSVGFSARFGEKLRDERKGQFKDFVTTEDIVLKSVNEVGLFLYFAGTN
SWLELVDENGKTLNEWTVVE
Seq ID 254
MNRWKVYATVIACMLFGWIGVEAHASEFNFAVTPTIPENQVDKSKTYFDLKMAPGAKQTVEIQLRNDTDEDITIENTVNSA
TTNLNGVVEYGQNGIKPDKTLRFNLKDYVEAPKEIILPKHSQKTLPLTITMPKDSFDGVMAGGITLKEKKKETTTSADQSK
GLAINNEYSYVVAIILQQNETKVQPDLKLLGVKPGQVNARNVINVSLQNPQAAYLNQLHLINTVSKGGETLYQSDTEDMQV
APNSNFSYPISLKGERLTPGKYVLKSTAYGVKDEKGTYQVKGANGEERYLYKWEFTKEFTISGDVAKELNEKDVTIKGTNW
WLYLLIALIILALLLLIFFLYRKKKKEEEQQSEQ
Seq ID 255
MSKNFWATLPKPFFVLAPMEDVTDVVFRHVVKEAGAPDVFFTEFTNSDSYCHPEGKDSVRGRLVFTEDEQPMVAHIWGDKP
EFFREMSIGVAEMGFQGLDINMGCPVPNVAERGKGSGLILRPEVAAELIDAAKAGGLPVSVKTRIGFTEMAEMEAWITHLL
EQDIANLSIHLRTRKEMSKVDAHWEVIPQIMAIRDRVAPQTTITINGDIPDRQKGLELAEQYGVDGIMIGRGIFKNPYAFE
KEPKTHTPQELLGLLRLQLDLQDKYAELVPRSIVGLHRFFKIYVKGFPGASDLRAQLMNTKSTDEVRQLLATFETEHGVLD
Seq ID 256
MRYLDAEAIENIATGAAFLGTGGGGDPYIGKMMALSAIEENGPVKLVSPEEIAAEDFFLPAAMMGAPSVAIEKFPKGDEFV
RVFEKLGKYLDQETIAGTFPMEAGCVNSMIPIVVAAKLGIPLVDCDGMGRAFPELPMVTFHLNGMSATPMAITDEKGNIGI
METIDNTWTERLARVQTVEMGASALVSIYPATGKQLQDYGIHNIVTLSEEIGKVIRGTYADEQEKRQALVEVTDGFELFQG
KILDVEREVKGGFNLGRVKLSGLNSDAGSEAVVHFQNENLIAEKDGQVIAMTPDLICMVDLETLTPVTTESLKYGKRVQVM
GLKANAAWRTKKGIETVGPRYFGYEMDYQPLENLVAKEDK
Seq ID 257
VTIVLLLLLNVSIMFLLGQLLSENSPLAGVIWATKDAVAKELNTDLFWSWNKFVLPIFFLVDAAVLYWRLIRRYHQMQLRH
IISELHYIADGNYNHRIPFELSGDLAKVVTSINGLVDSTVAAIEDERRIEKSKDELITNVSHDIRTPLTSIIGYLGLIEDR
QFHSQEDLLKYTHTAYVKAKQMKLLVDDLFEYTKVRQPSVPIHTTTFDMAQLIEQLAADFELEAKKINMQIQVKANPASLM
MEGDTEKLVRVFNNLLSNALKYGKGGHHIVMEVDKVGTEAIIAVRNDGPAIPKHSLDQLFDRFYRVEESRSQETGGTGLGL
AIAQSIVALHGGYIYAKSDQKWTSFIIHLPLQRTNKKSES
Seq ID 258
MEAEALRAIVAENRQLEQNLTKRNEQYIFDLKKSLKAANLSEEELALALHGILPELVAGQKTGKTARQLFGTVSERTEAIL
NKPAEVKEPAGWMIWLDNTLLLLGLLTIMLAAMSLFSKGTAQPLGLTTYILGAMAGGYVFYLMHKYVYRFDRQGGDKSKRP
GWLKTTAILFGGMFLWIAVFAGSAMLPPVINPILDPMIALVIGALAFVARYFFKKKYNIQGSFMTRQ
Seq ID 259
MNLVLAEIAAMGLKNISIAPSSIANVHEPLIEHIKNGVVTNITSSGLRDKVGAAISAGIMENPVVIRSHGGRARAVAAGDI
HIDVAFLGAPSSDAYGNANGTKGKATCGSLGYAMVDAKYADQVVIITDTLVPYPNTPISIPQTDVDYVVEIDAIGDPDGIA
KGATRFTKNPKELLIAEYAAKIITHSPYYKEGFSFQTGTGGSSLAVTRFMREQMLKDGIKASFALGGITNAMVELLEEGLV
EKIIDVQDFDHPSAISLGENANHYEIDANMYASPLSKGAVINQLDTAILSALEVDTDFNVNVITGSDGVIRGASGGHSDTS
MACKMSLVIAPLVRGRIPTIVEQVNTVVTPGTSVDVVVTEVGIAINPKRTDLIDCFKTLDVPQFTLEELKDKAYNIVGTPE
PIKYGDKVVALIEYRDGSLIDVVRNV
Seq ID 260
MTMNPFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHTT
IYRWVQEYGKLLYQIWKKKNKQSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWLRKKRDTQAAYAFLKRLVKQFDEP
KVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLNNLIEQDHRPVRRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGT
LFGFSVCTEIKVLLGIPA
Seq ID 261
MNKKRIIFGLLSFFLPIFLVFGGLLFFLLLLTSTSDTSKNDCIQPSINNPTDATDTPKSIEQFVKSHKDAYLLSWKAGGFL
PSASISQTMVENGFNFTNPSGTSFWQAHNMGGVKTSKKEDFPVTLATFGQDSVDISGTKPGSNVGDGTGGAYTWFKDYNAG
IVGKAEFMAHQTLYTGAINNTDGLSTLSAIYSGGWATDPTYLMKLQATYNSLGKQFQWLDQEAIQKYGNAPFKKSELYLNI
PGKSPITNEKYGKNSDCVVTSDTSDQVTGQNTAPSLEVPSAYKGKLTLPPIDSNDYAGNNYPFGQCTWYAYNRMAQIGKPI
EWFSGDGGNGAGWANSARAKGYTVVKVKPSVGWAASMQGGIGGSAPPYGHVAVVEYVNSDGSILVSEANVINQGSGTRSWR
VLDRATVEQIDFIQGKGA
Seq ID 262
LNLQIKIKMEEIMMKKNLFASLFSATLLFGGSEISAFAQEIIPDDTTTPPIEVPTEPSTPEKPTDPTPPIEPPVDPVEPPI
TPTEPTEPTEPTTPTEPEKPVVPTEPTTPTEPTTPTEPSEPEQPTEPSKPVEPEKPVTPSKPAEPEKPVTPTKPTEPEKPV
QPAEPSKPIDVVVTPTGELNHAGNGTQQPTVPIETSNLAEITHVPSVTTPITTTDGENIVAVEKGVPLTQTAEGLKPIQSS
YKVLPSGNVEVKGKDGKMKVLPYTGEEMNIFLSAVGGILSVVSGFVIFKKRKAKV
Seq ID 263
MKQQTEVKKRFKMYKAKKHWVVAPILFIGVLGVVGLATDDVQAAELDTQPGTTTVQPDNPDPQVGSTTPKTAVTEEATVQK
DTTSQPTKVEEVASEKNGAEQSSATPNDTTNAQQPTVGAEKSAQEQPVVSPETTNEPLGQPTEVAPAENEANKSTSIPKEF
ETPDVDKAVDEAKKDPNITVVEKPAEDLGNVSSKDLAAKEKEVDQLQKEQAKKIAQQAAELKAKNEKIAKENAEIAAKNKA
EKERYEKEVAEYNKHKNENGYVAKPVNKTLKFDREATKNSKVVSVKAAEYIDAKKLTDKHKDKKLLISMLSVDSSGLTTKD
SKKAHFYYNNGAGGTLVVLHKNQPVTITYGNLNASYLGKKIASAEFQYTVKATPDSKGRLNAFLHDDPVATIVYGINIDPR
TKKAGAEIEMLVRFFGEDGKEILPTKENPFVFSGASLNSRGENITYEFVKVGNTDTVHEINGSKVARHGNKVYSKTDIDVG
TNGISISDWEAVQGKEYIGATVISTPNRIKFTFGNEIVNNPGYDGNSMWFAFNTDLKAKSITPYQEKGRPKQPEKATIEFN
RYKANVVPVLVPNKEVTDGQKNINDLNVKRGDSLQYIVTGDTTELAKVDPKTVTKQGIRDTFDAEKVTIDLSKVKVYQADA
SLNEKDLKAVAAAINSGKAKDVTASYDLHLDQNTVTAMMKTNADDSVVLAMGYKYLLVLPFVVKNVEGDFENTAVQLTNDG
ETVTNTVINHVPGSNPSKDVKADKNGTVGSVSLHDKDIPLQTKIYYEVKSSERPANYGGITEEWGMNDVLDTTHDRFTGKW
HAITNYDLKVGDKTLKAGTDISAYILLENKDNKDLTFTMNQALLAALNEGSNKVGKQAWSVYLEVERIKTGDVENTQTENY
NKELVRSNTVVTHTPDDPKPTKAVHNEKGEDINHGKVARGDVLSYEMTWDLKGYDKDFAFDTVDLATGVSFFDDYDETKVT
PIKDLLRVKDSKGEDITNQFTISWDDAKGTVTISAKDPQAFILAHGGQELRVTLPTKVKANVSGDVYNLAEQNTFGQRIKT
NTVVNHIPKVNPKKDVVIKVGDKQSQNGATIKLGEKFFYEFTSSDIPAEYAGIVEEWSISDKLDVKHDKFSGQWSVFANST
FVLADGTKVNKGDDISKLFTMTFEQGVVKITASQAFLDAMNLKENKNVAHSWKAFIGVERIAAGDVYNTIEESFNNEKIKT
NTVVTHTPEKPQTPPEKTVIVPPTPKTPQAPVEPLVVEKASVVPELPQTGEKQNVLLTVAGSLAAMLGLAGLGFKRRKETK
Seq ID 264
MKYERPLKRESQIKEFELGTHAAVIEKVQKKRSQKGNDMFLLSLLGKSNEKGVYFLTFGNDYTEDNLRYILASIQDNGVEI
PDVDFGYNRETFEFLKGKDVYIQVEEQEYKGKVKHAVTNFLTQDEFEESEEMEFSESNTEEDW
Seq ID 265
LVEQREIVHRYYVLGTSTLDKVLKVLLNLSSDGVLALKDKFLPLKGENNLYKLMNREDPLTSAQLHEKVNLNKLKEQLEAQ
GLPFAFKETKEGTNFYFRVKDTELAKKALERVLTDIKKNPQMILRKPNTMTFDERLAYTAANKKYVGKIDQTKAITKGRKL
Seq ID 266
VRMNKKTPKPLTPPKAFFNWCTAQIPTYEWQNKKETILASSRKNCPIIKKRLTKYSRLSFPTKFYSFGIILVRAKRIEIQT
HSYWQTITDGIENLIYEPSNLERFSNDTHVKAHYENGHWHEGLLANYGFMSSAYTNTVFYPNNWQEKLTTVSELKYLQLPE
IERQELAHIYKYRNEIEFLQKIGATTLANEIIFDDYRNVFGLYFHKVDMRVITKKWLKANKQKLKTRNPTFHEFMLEKTLK
ERNAPMINEIEKYVHYSQIKQLPKEVNLTKFQKWFIRKGERRDYYMDYLHMLEELNTPLNNDSVLYPENLQVAHDNAMNTL
NLLKSEIEEKQYQERKNQIKALEAEIDDLLFLTPHSLQEIIQEGSILRHCVGSQHYIERHTQGKTTIVFIRRKEKPDMPYF
TLEYRNQQVIQIQGKCNRQEVPEKIKQAVRQWQENLQHALSS
Seq ID 267
PSEKGNQGRVEKRSDAKSTNHVNAGRYTE
Seq ID 268
ENEFKLHKIGLHSFLDRVHQSVVLGVRSLRSIKKKCNHYPQTEKQKQDKAGQAMRMRQGIVLGMCLIVLHS
Seq ID 269
NIFSKSSAPIIRRFIRSMARWDVCFLDDRSLDAGASFDGFCLPNVCYRYVDWDVNYGSWWYGNV
Seq ID 270
RGRCYIFNEVPRCWQENSPTNDFRFKRKIRRIRVFRSSGCGHDRNRSGDNKQSSIGRSVRSIKCPRLQ
Seq ID 271
TRLWGRQLCRKSQIRKSSGINYRR
Seq ID 272
LGLSFIKHVLAYGYVLWGNIHKRQIHWELMST
Seq ID 273
HLVFVHVKDVNQPGKSFLTRNGLLKLSNLLIGIQRNH
Seq ID 274
KTVVARQLRWKWRLQKQGLHRNKLGISMLMVRQPQRMTLRKQRPSNVSLVSEHRKFQFQVQRV
Seq ID 275
CYSKFRQTSKQWPNKRRQGD
Seq ID 276
VASPSKSAPFFTHAKKCQNQRRNSCFSFSSPSWRLCHLNV
Seq ID 277
NKGRQPIYRKLQRAPLILKYLSPAQVNQLIHNQLQNQLATKWQVVAKKFLGKQVIRSNIKHGALVQSFV
Seq ID 278
ESIKDFNNVSRKKQIKRVQSKWKIIFYSVVISLDSAVKPIGKFSDIFKEALFSNTTPKTKKSSVKEYVKI
Seq ID 279
HLWLVLRYLPKQNQASKHLLMKSEIQQQPLPIAMICMLPS
Seq ID 280
VMFRQNHRRDKKKKRRSEQRRKKPKLQNAKQKLKPLPNKNYKSGNAWNKRQPSA
Seq ID 281
RIANWNLEYTNNLEVILLGKKGSKIKKKKRRLKEKAIANGTYSKRGKSDGMYKMQGTDDCLGKR
Seq ID 282
KTNTALAGMATLAMMNGTTAKASSCPAPTIFPVAIMIRLR
Seq ID 283
RLPAPPLRKTRSLPSMPKSWNRCSPKT
Seq ID 284
GGGTHLKQYEIILADPPWRYSQKGGAGSGRTSLRHHEHGGFMLTAYQPFCCAGLRAFSLEYIPTASRSTASDSCMGIHL
Seq ID 285
RKQLITVVYKSKIQRFMSAIHGNQKRTLFQQQIKQVKTFPSKKSLFQVKLITPKQAFIQLFTVTKVKKKQPM
Seq ID 286
QELRIVVIQIQINQKRIRQQQMQAKKLKKYLILMLKLAIPSK
Seq ID 287
LSWQSSASRRCFGNRDKSIPSSRWRKLSNVPT
Seq ID 288
RNTQGKCRFSLRVSLFFGKVCRKVGSFAHELSIDFCRKPTNRNRTLDFKARHISRCRGYVRICFR
Seq ID 289
FPSGLLFARGNVGAGSASGDSSIRGTVEHATFNGSRTSGGTESARSI
Seq ID 290
NQRKKFNQNQNQSPLRKSQNRNNLRQPLRKIKKLRFLLKKKSLHPRKKTNLWRNRQCQKKKRNKQKKQRLIPYK
Seq ID 291
QQVCLAPLSHSLRAWESSNANVKTSSLL
Seq ID 292
AVMHDQKTNRQPLTQSTKKKPKNKQNMRSMVSLKILQNGTSGKLTMENLKIYYKKRNLLLTTKDF
Seq ID 293
PNSCVYHMNDQRIKFQATKNKIGPKMLPPIAKEAPKAPNIGRTVNQKTGSPTPAKGPIIPTLTP
Seq ID 294
IKCPTSRQLKHFFFHAFRQTCIPKAFNHINNGDYFFIISHFASVQIIRKVF
Seq ID 295
RHISNGGTFLAVTEMAIDDFDSRTFQIF
Seq ID 296
STLSIIALIFSKHCVVCSLIPPATNAFVLGSNGS
Seq ID 297
NKSSAFSPAKRCPPLTTTCLAPICTIRFAASSISATEEISPKPDKMPASIKLGVATNANGSKSFFRPSTLLGCRICAVPLQ
AMTGSITTF
Seq ID 298
LPLVVYNESNRKMDALENSCRQSRLSRQKQRPEWLFQLPLRIVLLHTK
Seq ID 299
TSNDSIMIAKLSNSFFKKFRSRTKQLIAASDCRTKTNNVASNPRIVDQLAPNVPQRMVKKPVVSNFTNKPTPAATKGPLKR
LRLCSILW
Seq ID 300
SALMSTTRPSGLSNKVTIFTDAALRYLNKSTKYANVRPLSMISSTIKICLPSTFVSKSLTIFTSPEETVALP
Seq ID 301
HVLQKYQRPQYLRYPRKIEPFPQFVICFLEFQPIQNL
Seq ID 302
MSIITPADTFLNRLINVAADSFKPFLSNVPVANIIIIKIAMPRLFTKVVPSVSSIDVKPSPPVKAVTIKATITIKIASNFN
AKPTMTINTPNKGNKSIKSLLLKIKFHFPLYANFRSFVRLF
Seq ID 303
RLNRSVLSLVPLLAVLGDKQKNHLAFFVKAAAVSSLIVHH
Seq ID 304
SVCIARHLPHLDELGHHKTNHHGRYYN
Seq ID 305
VAPNKVSGRVVNTVKLSSVPATLKVTLAPSERPIQFRCISLILSGQSTSSKSSNKRSA
Seq ID 306
MIPASIKMTRSATSFAKPISWVTTTIVIPSIAKFFITDSTSPTISGSKAEVGSSNSITSGRMAKARAIATRCCCPPES
Seq ID 307
HNPMKIEAKNAGIASSKSVQLMSLKEANIIIPTKINAGAVACAGTIAI
SGEKRLLRANRIATTTDVKPVRPPAPIPAALST
Seq ID 308
YFFVISTKYSSNNKFSSAGFFSYASVIFCKNCAPIIQPARQIFAISPNGKSHPYSSEAARSCAKPCA
Seq ID 309
PSSSINVTFVVVEPASIPKKVTPVC
Seq ID 310
AIINDVRPFRSCCSPSCNNSSVFVSILEVASSKIKILGSASKARANEINWRCPAESVLPRSSTTVS
Seq ID 311
SSSSGNTCGQKDSASQNNCSVTVPLNLGSSSSAKGKLATSAAV
Seq ID 312
IIPASTGECKASMPNIVKANAIANVYNKLMMGLNNNEFIRIIATRVFK
Seq ID 313
TNCIATPQAIPNFKSPKIMPQTNPPTKGRPIV
Seq ID 314
YPFQIDKSTPWSPFAANVLIGDTKNKTTSNVANSFFF
Seq ID 315
FFNDLRISIITFSLDISMPTKGSSKKITSAPWAKTRAINTRCNWPPESSPIWRCA
Seq ID 316
FLHKSHCPVEPKKTVPVPSLIC
Seq ID 317
DTNCCANSVAVTPTSSNRTMIV
Seq ID 318
GSPSSSYRVSTITSRVRVYCREKMRSISGWTSAKVKALNAVER
Seq ID 319
LLCHYCFFLIPQCDRNNRDSLCHVLGS
Seq ID 320
KTIAKVNNATNQFRAEPKSALPAPPAIYLIATGNKDSPMLKITVPVTKGGNNTRIFLMNKPTIITRILPTNWEPKIAAIPK
SAPMAIKIGTYAKLAPIITGNREPTFPKIGKS
Seq ID 321
PTLILKMKPFGSPSSNSLLSNFKTNHALTYSS
Seq ID 322
GTLSITVAPVCTMTAAESEDVPISKIAGPKEPVAKITAVDVEPTAKPIKIPEIGAKKVPEIPITEIKWTICVDKSETEINP
AIAPAPIKIKTVSVTLFKPEDA
Seq ID 323
TKSRANVDTLQEIYTIFLGGCFKRAVNNLRSKPVRGGSTTMASGLKPSLNHLGNKSSAVPTA
Seq ID 324
LSCETKKSTGPSLLTVCILIVYTKTITPIPLKAVAINKVKPVSVLLLLNK
Seq ID 325
FLFSLPVRREPPLKFHKQSSVLAPFVPITTIILQNQHLQQKLVLNRQRHTFG
Seq ID 326
NKPAKNAPKTAPVNANGGIPKNVIRIKPQTSAMSVP
Seq ID 327
INSQIAPMPINCLNLCADIIKSNHIQNNMHKSNVKKHWRDKPPVFMILSYQWTNHCSPIN
Seq ID 328
FSFSLYEFPHKKRQIIQQDRRR
Seq ID 329
AKNALIPKAGAVAIGYFANTPIKIQPKALAKAVAVKTAPSSMPEAPSTVGLTNKIYAIEINVVKPANISRFIFVFNCSSSK
YELIFSFMFRPPKI
Seq ID 330
LFSDTFSISLTAFVSLNSIIGNSRRNSDCKINEELIPLGQTISAFSFSCRCSFFCPKISPPNNMVKKPISSDGIDRFVNFS
KGANACSQR
Seq ID 331
INSHSIQRVIRVPCPKSSSGSKKSSSVS
Seq ID 332
VNQCLKRQIYRHIFLIHHSSGSCALLPFCNNFPYKINAPNKLNHHTERGIQMKDLFNHQLTKHQ
Seq ID 333
SAISIFSVPELMYKVTDRPFFTDDFASGSVLTTLPSSILSSGIFLVSLSTFSPALFSACEASAFVSFVTSGTSTSLPPDIA
KAKPPIAKEINAAIAKPIHNFLRFFFGGCSAKMSSAISSGTSTGVVSSGSAMSSGIGVNTFVSPNKAVGSHAGSFKRLADK
DVYKSSAISFAVL
Seq ID 334
GSPPPPVPKKAAPVAIFSIASASK
Seq ID 335
SPAITAPNAKEPTVIERPDIKLPAV
Seq ID 336
IRSRNDRLASGTLISFSVKCNTSANKMEKPMPNNIQPLMNGIFASGMLVLAPIAVTITPKKAPGFEIS
Seq ID 337
MFVNIGMNPIFIDRKAIKTDTIGATAIGINNKGLKITGSPKIKGSLMLKNAGTNEIFDMAFLLDECPTMAIAIAKPIVQPA
PPIVAN
Seq ID 338
QANSVRPMNSHAHLQADSNNPLLHVLD
Seq ID 339
VKLPVPSFEISCAFCSRLAHPDNSKVAAVSSSHFFPINSF
Seq ID 340
VIQQESRLRVADNSGAREILTIKVLGGSGRKTANIGDVIVATVKQATPGGVVKKGEVVKAVIVRTKSGARRADGSYIKFDE
NAAVIIRDDKSPRGTRIFGPVARELRENNFMKIVSLAPEVL
Seq ID 341
atggtaggga ttatcctagc aagtcatgga caatttgctg aaggaatctt acaatccggt 60
tcaatgatct ttggcgaaca agaaaacgtc aaagccgtga ttttgaaacc aagtgaaggt 120
ccagatgact tgagagccaa attggaagaa gcggttgctt cttttgataa tcaagacgaa 180
gtgttattct tagttgattt atggggcgga acaccgttca atcaatcaaa cacattattc 240
gaagagcaca aagataaatg ggcaatcgtc agcggtttga atttaccaat gttgattgaa 300
gcctacgcat cacgcttttc aatggaatct gctcatgaaa ttgcagcaca catcattgaa 360
acagcaaaag acggtgtaaa agtaaaaccg gaagaattag aaccggcaga agcaccgaaa 420
gcagcagttg aagacgcaca acctaaagga gcacttccag aaggaactgt ggtcggagac 480
ggaaaaatca aatatgtatt agcacgtgtt gactcacgtc tgttacatgg acaagttgcg 540
acagcttgga caaaagcagt gcaaccaaac cgtattatcg ttgtttcaga tgcagtatca 600
aaggatgatc tgcgtaagag actaatcgaa caagctgctc ctccaggtgt aaaagcaaat 660
gttattccta ttagcaaaat gatcgaagta gcaaaagacc ctcgttttgg aaataccaaa 720
gcattgcttt tatttgaaaa tcctgaagac gtattgacag ctgttgaagg tggcgtagac 780
atcaaagaat taaatgttgg gtcaatggcg cattcggtcg gtaaagtcgt tgtaagtaaa 840
gtattgtcta tgggacaaga agacgttgaa gcgtttgaga agcttgaaca aaaaggtgtg 900
aaattcgatg tacgtaaagt acctaatgat tcacgcgata acatggatga tattttgaaa 960
aaagcaaaag ctgaattagc aaaagcgtaa 990
Seq ID 342
atgattatta aagaagagat ttttgtacct aaagaattag tgaaagaaaa aattgacctg 60
ctcgttgaaa acctgacaac gatcaaagac gataatggtg aatttttgtt ggactttgat 120
ggactgaaag tagatgacaa aagctggaca gtgtggaatt ggccacaagg tgtcggtcta 180
tatggtattt ataaaaatta ccagatgaca aaaaatcctc gcgcttatca agtggtcaat 240
gaatggtttg aggatcgtat ggaagaaggc gctccgccca aaaacgtcaa tacaatggca 300
ccgctattga ctatggctta cttgtatgaa gacacgaaag attcacgcta tttgccttat 360
ttagaacaat gggcagaatg ggtcatgaat gacatgcctc ggacaaatga agacgggtta 420
cagcatgcca catatggacc ggaaaataag aatcagttgt gggacgatac attgatgatg 480
acagtgcttc ctttggcaaa aatcgggaaa ctgttgaatc gcccagaata tttagaagaa 540
gctcgttacc agtttatgat ccatatcaaa tacttgatgg ataaacgaac aggattgtgg 600
tatcacggct ggacgtttga aggaaatcat aactatgccg aagcattttg ggctcgtgga 660
aactgctggt tgacgattgc tattccagag atcattgaaa tcttagaatt atcgaaagaa 720
gatgcattgc gcaaattatt gatcgaaaca cttgaagcac aagtgcgtgc attaaaaact 780
tatcagtctg aaagcggttt atggcatacg ttgttggatg atccttcttc ttacgtagaa 840
tcttctgcaa ctgcaggatt tgcttacggt attttgaaag cggtccataa acgttattta 900
ccacaagaat ataaagaagt cgcttatcgt gctatccaag gattgcttga acaaatcgat 960
gaaaaaggag aagttcaaaa cgtttcaatc gggacaggaa tgggggacag tttagatttt 1020
tatcggaata tcggaatcac agcaatgcct tatggacagt cgttaacggt gctttgtttg 1080
acagaattgc tcgtctctta ttgttaa 1107
Seq ID 343
atgaaaatag caattgcagg cgctggcgca atgggaagtc gctttgggtt aatgcttcac 60
caaagtggga acgaagtatt attaatcgat ggctgggcag aacatgtcca acaaatcaaa 120
gagcatggat tacaagcaaa ttttaatggt aaagaggtag aagcaaaact accaatcgtt 180
cttcaatccg aagtagaaaa agaagatcaa gttgatctga ttattctatt taccaaagcg 240
atgcagctgg aaaaaatgct gcaggatatc caatcattaa tcaaaaaaga tacagaggtc 300
ttatgtctat taaatggtat cgggcatgaa gatattattg agaaatttgt accaatggaa 360
aatatctata ttggaaatac catgtggacg gcaggtcttg aaggtcctgg tcaggtcaaa 420
ttatttggaa gtggttcagt agaattacaa aatttaggtg atggaaaaga agcagctgca 480
aaaaaattag cagacaaatt gtctgaatca ggattaaatg ctcatttttc tgacaatatt 540
cactattcta tttatcgcaa agcatgtgtg aacggaacaa tgaacggact atgcacaatc 600
ttagacgtta atatggctga gctaggaaaa acatcgactg ctcataaaat ggtggcgacg 660
attgtcaatg agtttgccaa agtagcagca gtagagaaag ttgaactaga tgtcccagaa 720
gtcattgcac attgtgaatc ttgttttgac ccggaaacaa ttgggttaca ttatccttca 780
atgtatcaag acttgattaa gaaccatcga ttaacagaaa tcgattatat caatggcgca 840
atttctagaa aagggaagaa atatggtgtt gcgacgcctt attgtgattt cttgactgag 900
cttgtccatg caaaagaaga tagtttgaac gtaaaataa 939
Seq ID 344
atgacttttc cttattcaga aggaaataaa agatatcatt cttggaatta cgctttacga 60
aatgagtttg gcgggaaaat tttcaaggtg ccgatcgatg gaggatttga ctgtccgaat 120
cgagatggga ctgttgccca tggcggttgt actttttgca gtgtatctgg ttcgggagac 180
atgatcgttg cacctgaaga ccctcttcca atccaattcc agaaagaaat cgacatgatg 240
catcaaaaat ggcctcatgt ccaacaatac atcgtttatt tccaaaattt tacaaacacc 300
catgcgccag ttgaaataat caaagaacga tttgagcaag tagtcaattt accaggtgtc 360
gtaggattat cagtcggtac acgaccggat tgtctgccag atgaagtagt tgattacctt 420
gctgaactga acgagcggat gtatttatgg gtagaacttg gattgcaaac aacttttgaa 480
gaaacttcaa aactgatcaa ccgagcacat gattatcaaa cttatttaga tggcgtggct 540
aaattacgca aacataatat ccgtgtttgt acgcatttga tcaacggatt accaggcgaa 600
agtttagaaa tgatgaaaga aaatgtcaga cgcaccattt tagattcaga tatccaagga 660
atcaagctcc atttgatgca tctgatgcgc aaaacgagaa tgttgcggga ctatcatgaa 720
ggtcgccttc aattaatgag ccgtcctgac tatgtcaatg tcatttgcga ccagctggaa 780
atgataccaa aagagatcat catccatcgt ttgacagggg atgcgccgtg ggattccttg 840
attggaccaa tgtggagctt gaaaaaatgg gaagtcttga atgcaattga tgaagaactt 900
cttcgcagag atagcttcca aggaaaatat gatgtacgga aaaaggtgag cgtctga 957
Seq ID 345
atggcaaaaa caggaatgta tgtaggcctt gatattggaa cgacatctgt taaagttgtc 60
gtggctgaat atatcgacag ccaaatgaat attattggag taggaaacgc aaaatcagaa 120
gggattaacc gaggcattat cgttgatatc gataaaacgg tccaagcgat acaacgagca 180
gtacgacaag cagaagaaaa agcaggtatc cagatcaaag gcgtaagtgt cggtttgcca 240
gcaaacatgc ttgaagtaga aaattgccag ggaatgattg cagttaacgg agattcaaag 300
gaaatcaccg atgaagatgt cagaaatgtg gcttcagctg cccttgttcg ttcaatacct 360
cctgaacgtc aaatcgtgtc aatattgcca caagatttta ctgtcgatgg gttcgaggga 420
atcaaagatc ctcgtggaat gatcggcgta agattagaaa tgtacggtct gttgtttaca 480
ggacctaaaa cgatcatcca taacatacgt aaatgcgtgg aaaatgccgg attaatcgtc 540
aacgaaatgg tgattactcc tttagcgctg actgaatcaa ttctttctga cggagaaaaa 600
gattttggta cgatcgtcat tgatatgggt ggcggccaaa caacaacggc tgttatgcat 660
gataagcaat tgaagtttac caatcttgac caagaaggtg gcgaatttgt cactaaagat 720
atctctattg tattaaatac gtcatttaac aatgcagaag cgttgaaaat aaactacggg 780
gatgcatacc cagaacggac atcaccagat gaagaatttc cagtagatgt gattggtcag 840
tcagaaccag tgaaagtcga tgaacgctac ttgtcagaaa ttatttcagc tcgaatggaa 900
cagattttca acaaagcaaa agatgcattg gaccaaatcg aagcattaga gttgccaggt 960
ggcgtcgtat tgacaggcgg agctgctagt cttcctggtg ttgtggactt ggcacaagaa 1020
atcttcggcg taaatgtaaa attgtatgtt ccaaatcaca tggggctgag aaacccggta 1080
tttacaaatg taataagtat cgtggattat tcagcaaact tgagtgaagt gtatcaattg 1140
gcaaaaattg ctgttacggg tgaaacctca gcagctcgtc aaatggtagt tgaacaagaa 1200
acaacaaaca catatgagag ctatgaagca cctgaagaaa caatctacga tgagccggaa 1260
ccaaaagagt ccggagaaaa tgtaaaaaat aaaatcaagg gtttctttac aaatattttt 1320
gattaa 1326
Seq ID 346
atggcaaaaa aaacaatcat gttagtttgt tcagcaggga tgagtacgag tttattggtc 60
acaaaaatgc aaaaagcagc agaagaaaaa gggatggaag ccgatatttt tgcggtctct 120
gcctcagatg cagataataa tttagaatct aagaatgtcg atgtattatt attaggtcct 180
caagtacgtt tcatgaaagc tcaatttgaa caaaaacttg cgcctaaagg tattccatta 240
gatgtcatca atatgcaaga ctatggcatg atgaacggag aaaaagttct tgcacaagca 300
gaaaatctaa tgaaataa 318
Seq ID 347
atgaaaaatc ataaaaaaat aaacgttatg ttaggagtcc ttttccttat tttaccatta 60
ctcacaaaca gcttcggcgc aaaaaaagtg tttgcagagg agacagcagc tcaagtcatc 120
cttcataaaa agaaaatgac tgatttaccc gatcctttaa tccaaaacag cgggaaagaa 180
atgagcgaat tcgatcaata ccaaggatta gccgatattt cattttcagt ttataacgtc 240
actcaagaat tttatgcgca acgagataaa ggagcgtccg tggatgcagc aaaacaagca 300
gtccagtctt tgactcctgg tacaccagtt gcttcaggaa cgacagatgc tgatggaaat 360
gtcactttat ctttacctaa aaaacaaaat gggaaagatg cagtctacac gatcaaagaa 420
gaaccaaaag acggagtgtc agctgccgca aacatggttt tagctttccc tgtatatgag 480
atgatcaaac aagcagatgg ctcttataaa tacgggacag aagaactaga tactatccat 540
ctctacccta aaaatacagt cggtaatgat ggaacgttga aagttacaaa aatcggtact 600
gccgaaaacg aagcactaaa tggagcagaa tttattattt ctaaagaaga aggaacacca 660
agcgtcaaaa aatacatcca aagtgtcaca gatggattgt acacttggac aactgatcaa 720
accaaagcca aacatttcat tactggtcat tcttatgaca tcggcaacaa tgactttgcc 780
gaggcatcta ttgaaaaagg ccagttgatc gttaatcatt tagaagttgg aaaatataat 840
ttagaagaag taaaagctcc tgataatgcg gaaatgattg aaaagcaaac aatcacgcct 900
tttgagatcc tggcaaatag ccaaacacca gtagaaaaga ccatcaaaaa tgatacgtct 960
aaagttgata aaacaacacc tcaattgaat ggaaaagatg tcgcaatcgg tgaaaaaatt 1020
caatatgaga tttctgtcaa tatcccatta ggtatcgctg ataaagaagg aacgcaaaac 1080
aagtacacaa cattcaaact tatcgatact catgacgctg ctttaacatt tgataatgat 1140
tcttcaggaa cgtatgctta tgccttatat gatggaaata aagaaatcga cccagtaaat 1200
tattctgtca ctgagcaaac agacggattc acggtttcag ttgatccgaa ttatattcct 1260
tcattaactc ctggcggtac attgaaattc gtttactata tgcatttgaa cgaaaaagca 1320
gatccaacca aaggattttc taaccaagca aatgtcgata acgggcatac aaatgatcaa 1380
acaccaccgt cagtcgacgt cgttactggg ggcaaacgat ttgttaaagt agatggtgac 1440
gttacatcag accaaacact tgctggagca gaattcgtcg ttcgtgatca agatagtgac 1500
acagcgaaat atttatcgat cgacccatcc acaaaagccg tcagctgggt atcggcgaaa 1560
gaatcagcaa cggtttttac aaccacaagt aacggtttaa tcgatgtgac aggtctaaaa 1620
tatggcacgt actatctgga agaaacgaaa gcgccagaaa aatatgttcc attaacaaac 1680
cgtgtagcat ttactatcga tgaacaatct tatgtaacag caggacagtt gatttctcct 1740
gaaaaaatac caaataaaca caaaggtaca cttccttcaa caggcggtaa gggaatctat 1800
gtgtatatcg gtgcaggagt agtccttcta ctgattgctg gactgtactt tgctagacgc 1860
aagcacagtc agatttag 1878
Seq ID 348
atgaataacc gaatccttga aacattggaa tttgaaaaag tcaaacagat ggtcagacaa 60
tttgtagtga ctgcacaagg aaaagaagaa ctggcagaat tggtaccagt gagtgagaaa 120
caaacgatca caaactggct tcaagaaact gaggatggat tgaaagtgca acgtctgcga 180
ggcgggatcc ctatacctaa acttgaaaat atccgtcccc atatgaaacg gatcgagatc 240
ggagcagact tgaatggagt ggagcttgca caagtttcca gagtattgtc tacgacaagc 300
gaattgaatc gtttcattga tgatctttct gacagtgaaa tcgaatttgc acgtttgtat 360
atgtgggcag atcagttagt cacgatccct gttttgagcc gccgtttaaa ggaagcaatc 420
gatgaagacg gacgcgtgac tgatgacgct tctcctgagt taaagagcat aagacagaac 480
attcggcgaa gtgaacaagc tgttcgtgaa cagttagatg ggatcgtccg tgggaaaaat 540
gcaaagtatt taagtgatgc gatcatcacc atgcgaaatg accgctacgt tatcccagtc 600
aaacaagaat accgcggtgt atttggcggt gtagtccacg atcaaagtgc ttcaggacag 660
actttattta ttgaaccaaa acaagtggtg gatttgaata atcgtttgcg tcaataccaa 720
atcgctgaac gaaatgagat tcaacgtatt ttaagtgaac tttctgctga acttgttccc 780
catcgtcaag aaatcatcca caatgcttat gtgattggta aaatggatct gatgaatgca 840
aaagcacgat ttggtaaaga agtaaaagcc atcgtgccgg gaatcagtga agacaatcat 900
gtcgtcttga aacaagcaag acatccactg atcgatcagg aaaaagtagt ttctaatgac 960
atcacaatcg gaaaagacta tcaagccatc gtcattacag gacctaacac aggtgggaaa 1020
acgatcacgt tgaagacttt aggtctgtta caactgatgg ggcaagcagg attaccgatt 1080
cctgcaggtg aagaaagtca aatcggtatt tttgaggaag tatttgcgga tataggagac 1140
gaacagtcga ttgagcaaag tctatcgacc ttttcttctc atatgacgaa cactgtagat 1200
attttatcaa aagtcaatga aaaaagtctg gtattatttg acgagctcgg tgcaggaaca 1260
gatccgcaag aaggtgctgc tctagcaatc gcaatcttag atgatcttgg taaaaagtct 1320
gcttatgtga tggcaacaac gcattatcct gaattgaaag tttatggata caatcgggca 1380
aatacgatta atgccagcat ggagttcgat gtggatacac taagtccaac gtatcgtttg 1440
ctgattggcg ttcctggacg aagcaatgcg tttgaaatct ctagccgatt aggtcttgat 1500
acagaagtta ttgatgaagc gaagcagttg atgaatgatg agagccaaga tttaaacgaa 1560
atgatcacag atttagagaa tcgccggaaa atggcagaga ccgaatatct ggaaacgcgt 1620
cattttgttt ctgaggcaca agaacttcac gatgatttga aagaagctta cagctatttc 1680
tttgaagaac gtgaaaaaga aatggaaaaa gcaaagaaaa aagcaaacga agtcgtgtca 1740
gaggcggaag aaaaagcaga aaaaatcatt gctgatattc gtaaaatgca acagcagatt 1800
gggcaaggaa atgtgaaaga gcatcagttg atcgatgcaa agacccaatt agcaaatcta 1860
catcaagaag aaactttgaa gaaaaataaa gtattgaaga aggcgaaaga acaaaaaacg 1920
ttgaaaccgg gagacgaagt gttggttacg acttacggcc aaagagggac gcttttacgt 1980
aaaaacggga atcagtggca agtagaaatc ggtatattaa aaatgaatgt gtccgaagac 2040
gagctgacgc ctgttgctcc tcaaaaagag ccgacacaac gagttatcca tgctgtacga 2100
tctgaatcta gtagtcacgt gccgaatcaa ttggaccttc gaggaaaacg atacgaagaa 2160
gcgctaagtg aggtagatca atatttggat tctgccattc tagctggtta tcctcaagtg 2220
actattgtcc acggaaaagg aacaggcgca cttcgcaagg gaattactga ttatcttaaa 2280
aaccatcgga gcgtcaaaag ctttgagttt gcaccggcaa accaaggcgg aaatggtgcg 2340
acaattgtta aatttaagta a 2361
Scq ID 349
atgttcgatg gctactgttc acgtgtcgta aatggcaatc caaacgttaa accagcaaca 60
agaaaaaaag tcttggaagt catcgatcgc ttggattatc gtccaaacgc agttgcgcgt 120
ggcttggcaa gtaagaaaac aacaacagtt ggggtaatca tccctgatgt cagcaatatg 180
ttctttgctt ctttagcacg tggtatcgat gatgtggcaa caatgtacaa atacaatatt 240
atcttagcaa attcagatgg caacgaccaa aaagaagtca atgtattgaa taacttactg 300
gctaagcaag tggacggcgt gatcttcatg ggtcaccata tcacagacga aatccgtgga 360
gaattttcac gttcaaaaac tcctgttgta ttagctggct ctattgatcc agatgaacaa 420
gtaggtagcg taaacattga ttatgtatcc gcaacaaaag atgcagtaaa taaattggca 480
aaaagcggca ataaaaaaat cgcttttgtc agtggtgcgt tgatcgatcc tatcaatgga 540
caaaatcgtc tgaaaggcta taaagaagca ttaagcgaaa ataacctacc atatagcgaa 600
ggattgatct ttgaagcaca atacaacttc aaagatggtc tatctctagc cgatcggatc 660
cataatagcg gtgcaacagc tgtctatgtt tcagatgatg aattagccat cggtattttg 720
gatggtttat tagatcgtgg cgtgaaagta ccggaaggat tcgaaatcat cacaagtaac 780
aattcactac tgacagaagt agctcgtcct cgtttaacaa gtatcacaca accattatac 840
gatttagggg cggtatctat gcgcctattg acgaaaatga tgaataaaga agaagtcgaa 900
gaaaaaacga ttatcttgcc ttatggaatc gaagaaaaag gatctacaaa ataa 954
Seq ID 350
atgaaaaaaa ttgatatttt atctcgattg aaaaacgccg gagtgattgc ggtggtacgc 60
ggaaaaagca aagaagaagc tttgaatgct tgccacgcaa tcatcaaagg tggtctaact 120
ggtattgagt tgacttttac agtccctcaa gcagatcaag taatcaaaga acttttgtca 180
ttctacaaag accaaccaga aatagtgatt ggcgcaggca cagtattaga cgctgtcaca 240
gctcgcttgg ctatcttagc tggcgcagag tatattgtta gcccttcctt tgatcaagaa 300
acggcagaga tgtgcaatct gtatcaaatt ccttacttac caggatgtat gactattacc 360
gagatcaaaa cagcgctgaa aagcggggta gatatcgtca aactatttcc tggaagcgct 420
tatggaccaa gtatcatttc tgccttcaag gcgccgatgc ctcaagtcaa tatcatgcca 480
actggcggtg tgagtctgga caatatgaaa gagtggttcg atgctggtgt cgtaacagtc 540
ggagtaggtg ggaacctttt agcacccgca gctacaggag attttgacaa agtaacggaa 600
gtcgcgcaac agtatgcagc aaaaatgaaa gaaatcaaga ggtag 645
Seq ID 351
atggatagtg tagtggtcca tcgtaaatcg cgtttatggg aatttacgct gcatttttcc 60
aaaattttgc ctattatgct atatcggtca ttgactcagc atctgactat cgcttttaaa 120
gatattgcac aagtcaaact gaatatctta gcagatgacc agacattcga tgaacagctc 180
ttgcaagatt actgggccca agctttggaa aatcagcaat gcgatacacc actagcacag 240
caagtattga aaacgcaggt acctgtgata aaagatcgta aagtcatctt gccaatcgac 300
agtacaggag ctatttctta tttgaagcaa caatacttgc cattagtaga agagttgttc 360
gtcagttacg gattcccaaa atttcgtatc gaaccagaag tggatgagca gcaagcggaa 420
cgagtattga aactttttga agaacgcaaa caagaacagg cagaagcctt tatgaagcaa 480
gcagctgaaa gtctagttgt ccacgaacaa aagaaaaagg aaagaaaaga gcaatcgcca 540
gctcttgaag gacccatcca catccagcta ggacgaaata ttccagccga tgaaccaacg 600
acaccaatga tcagtatcgt tgaagaagaa cgaagagtca cacttgaggg gtacgtattc 660
gacaaagaag tcagagaact acgttcaaaa cgcaagatct tgactttgaa aatcacagat 720
tatacttcat catttatcgt caaaaaattc tcgaacggcg aaaaagatga acaagtattt 780
gatgcgatca gtgtcggtag ctggttaaag gtacgaggaa gtattcaaga agatacgttt 840
gtccgtgatt tggtcatgaa tgcgcaagat atcatagaag tcaaacatac acctcgcaaa 900
gattatgctc ctgaagggga aaaacgagta gagcttcatg ttcatagcaa tatgagtacg 960
atggatgcaa caaacagcat ctctgatctt gtggcacaag cagggaaatg gggacaccga 1020
gctatcgcca ttacagacca cggtggtgcg caagcctttc cggaagcaca tagtgctgga 1080
aaaaaagcag gtgtgaaaat cttatacggc gtggaagcga atgtcgtaga tgatggtgtc 1140
ccaattgctt ataatgatgc acatgaagca ttgagtgaag ccacatatgt cgtatttgac 1200
gtggaaacga caggattgtc tgcagtatat gacacgatca tcgagctagc cgcagtcaaa 1260
atgtataaag gaaatgtgat cgaaagcttt gatgaattta tcgatccagg acatcctttg 1320
tcacgaacaa cggtagatct gactggtatc acggatggaa tggttcgagg atcaaagtcg 1380
gaagaagaag tattgcggat gttccttgaa ttttcaaaag atacaatttt ggtagcccac 1440
aacgcggctt ttgatatggg gttcttgaat actagctacg caaggtatgg tattcctgaa 1500
gcggccaatc cagtcattga tacgttggaa ttagctagat atctgtatcc tcaattcaaa 1560
cgtttcggat taggtgtgtt atctaaaaaa tttggtgtca gcttggaaca gcatcaccga 1620
gcaatctatg atgctgaagc aactggtcac ttagcatgga tctttgtgaa agaggcaatg 1680
gacaatcata acatgcttta tcatgatcag ctaaatgagc atatcggaga aggagattct 1740
tacaaaaggg ctcgaccttt ccatgtaacg attttagcaa aaaatcaggc aggactgaaa 1800
gatttgttca aactgatttc aatgtcgaat gtcgaatact tcgaacgcgt cccacgaata 1860
ccacgctccc aattgaaaaa gatgcgtgaa aatctattga tcggctcagc ctgtgataag 1920
ggagaaatat tcgaagccat gatgcAaaaa ggggtagaag aggcaagaaa ccgagcaaaa 1980
ttctacgatt atattgaagt tatgccaaaa gcggtctatg cgcctttgat cgagcaagaa 2040
ttggtaaaaa atgaacatga tttggaagaa attatccaaa atttggtaga aatcggaaaa 2100
agtttggata aaatcgttgt tgcaacaggt naagtacatt atctgaacga agaagatgcc 2160
atttaccgaa aaattttgat caattctatg ggcggagcga atccgttgaa tcgtcatagt 2220
ttgccggatg tccatttccg aacaaccgat gagatgctaa ctgcatttca ctttttaggg 2280
gaagaaacag cgaaagagat cgtagtagaa aatacgaata aaatcgcaga tatctgcgaa 2340
gaagtgattc cagtcaaaga tgaactttat acaccgaaga ttccaggttc agaagatgaa 2400
atatcagaat tgagttacac aaaagcaaaa caaatgtacg gagatccgtt acctgagatc 2460
attcaaaaac gattaaaaaa agagttgaat tctattaatg gtaatggctt ctcggttatt 2520
tatctgatcg cccaaaaact agtgcataaa agtaatgaag atggctatct agtaggttca 2580
cgtggctctg ttggctctag ctttgttgcg acgatgacag gaatcactga agttaatccg 2640
ttagcccctc attattattg tccagagtgc caatattccg agttttttga agatggtacg 2700
tatggttctg gatttgacat gccagaaaaa caatgtccaa aatgtggtgc acgcttaaac 2760
aaagacggtc atgatattcc atttgagaca tttttaggat tccatggaga caaagtgccc 2820
gatattgatt tgaacttctc aggtgattat caagcagaag cccataacta tacgaaagta 2880
ttgtttggcg aagactatgt ttaccgtgcc ggaacgattg gtacagtcgc cgacaaaaca 2940
gcatatggct atgtaaaagg atacgaaagg gacaataatc tgcagtttcg aagtgcagaa 3000
gtcgacagat tagcaaaagg cgctactgga gtcaaacgga caaccggcca gcatccaggg 3060
gggatcatcg ttattcccga ttatatggat gtctatgatt ttacgccgat ccaatatcca 3120
gcagacgatc aaaattcgga atggaaaacg actcattttg attttcactc gattcacgat 3180
aatgtattga aattagatat tctagggcat gatgacccaa ctgttattcg gatgcttcaa 3240
gatttatcag ggatcgatcc acaaacgatc cctacggatg acccggaagt gatgcggata 3300
tttgccggtc ctgaagtact tggagtaagt caggaacaaa tttattccaa aacagggaca 3360
ttaggtattc cagaattcgg cactcgtttt gttcgaggga tgctagaaga aacgcatcct 3420
acaacttttg cagaactact gcaaatttcc ggactatctc atggtacaga cgtatggtta 3480
ggaaatgcgg aagaattgat tcgacgagga gatgcgacac tggcggaagt aatcggctgt 3540
cgtgatgata ttatggttta tttgatccat gctggattag atagcgggat ggcatttaaa 3600
atcatggaaa ctgttcgtaa aggacaatgg aacaagatcc ctgatgaatt gcgagaaacc 3660
tatctatcgg cgatgaaaga aaataatgtg cccgactggt atatcgattc ttgttcaaaa 3720
atcaaatata tgtttccgaa agcccatgct gcagcttacg tattgatggc tcttcgtgtc 3780
gcttatttca aggtttattt ccctattttg tattactgtg catacttttc tgtacgtgca 3840
gacgactttg atctagtctc catgtgcaaa ggaaaagatg ctgtgaaaca agcaatgaaa 3900
gaaatcacgg acaaaggatt agatgcttcc gtaaaagaaa agaatcaact gacagtgctg 3960
gagttagcca atgaaatgct ggaacgagga ttcaaatttg gaatgattga tttgtataaa 4020
tcggatgccg ttaactttgt tattgaaggt gatacgttga tcgcaccatt tagagctgtc 4080
ccaagtttgg gaaccaacgt ggcaaaacaa atcgttgaag caagaaaaga cggtccattt 4140
ttatccaaag aagatcttgc cacccgcggt aaagtttcaa aaacattgat cgaatatatg 4200
aacgacaatg gcgtattgaa agatctacca gatgaaaatc aattatcgtt attcgatatg 4260
ctataa 4266
Seq ID 352
atggttttat gggtaattaa tatcattttg ctattgattg tgcttgcaat catcctttgg 60
gaagtctatc tacgagtaat ggccaaacgt tcagcaacga ctttgacaga agaagaattt 120
cgagaaggaa tgagaaaagc acaagtaatc gatgttcgcg aaaaagatgt gtttgatgct 180
gggcatattt taggtgcaag aaacatccct tacactgttt tgaaagattc attaggatct 240
attcgaaaag accagcctgt ctatatttac gatcaaaaga aaagtttgag catccgtaca 300
gcaaataaat tacgtaaagc aggttatcaa gagatttatt tacttaaggg tggatatgac 360
ggatggtctg gaaaaatcaa aagtaaaaaa gtataa 396
Seq ID 353
atgataaaaa ttacttttcc agatggtgca gtcaaagaat ttgagtctgg aacaacaaca 60
ttggcaattg ctgaaagcat ttcaaaaagc ttagcaaaaa aagctttagc tggtaaagtg 120
aacggaaaac tcgttgattt gactcgtcca atcgaggaag atgcaagtat tgagatcatc 180
acaccagacc atgaagatgc acttggctta gtccgtcatt cagcagctca tttgatggcc 240
caagccatgc gtcgcctcta tccaaatatc cactttggcg ttggaccagc tatcgattca 300
gggttttact acgatacaga taatggacaa aaccaagtaa ctgcagaaga tttgccggca 360
atcgaagcag aaatgatgaa aatcgtcaaa gaaaatctgc cgatcgaacg ccgtgttctt 420
tcaaaacagg aagcattaga aatttttgca agtgatccgt acaaagtaga actgattagt 480
gaattgccag aagaagaagt gattactgct tatcaacaag gcgaattcat cgatttgtgt 540
cgtggaccac atgttccatc cactggccgt atacaagtat ttaaattatt atcagtggca 600
ggtgcttatt ggcgtggaaa ctcaaacaat caaatgatgc aacgtgtata cgggacagcc 660
ttttttgaca agaaagcttt gaaagaatat atccgtctcc gtgaagaagc aaaagaaaga 720
gaccatcgta aactaggaaa agaacttgac ttgttcatgg tttccccaga agtaggttct 780
ggtttgccat tctggcttcc aaaaggggca acaatccgac gcacgatcga acgttatatc 840
gtagacaaag aagttagtct aggttaccaa catgtatata caccaataat gggagatgtt 900
gagttgtaca aaacatctgg acactgggat cattatcaag aagacatgtt cccgccaatg 960
gatatgggag atggcgaaat gcttgttctt cgcccaatga actgccctca tcacatgatg 1020
gtctacaaaa acacgatcca ttcttatcgt gaattaccaa ttcgtatcgc tgaattagga 1080
atgatgcatc gctatgaaaa atcaggtgct ttgtcaggtt tacaacgtgt acgagaaatg 1140
acgttgaatg atggtcatac atttgttcgt cctgatcaaa tcaaagatga atttaaacgt 1200
acgcttgaat tgatggtggc agtatatgct gacttcaata ttacagatta tcgtttccgc 1260
ttgagctatc gcgatccaaa caatacagat aaatattttg atgatgacgc aatgtgggaa 1320
aaagcccaag caatgctgaa agccgctatg gatgaattag aactagatta ctttgaagca 1380
gaaggcgagg cagcctttta cggaccaaaa ctagatgttc aagtcaaaac agctcttggt 1440
acagaagaaa cattatcaac tatacaatta gacttcttat tgccagagcg ctttgatttg 1500
acatatgttg gagaagacgg ggaaaatacg catcgtccag tagttatcca ccgcggaatt 1560
gtttctacaa tggaacgttt tgtcgcttat ttaacagaag tatacaaagg cgcttttcca 1620
acatggttgg cacctataca agcaacaatc atccctgttt ctgttgatgc acatggcgat 1680
tatgcttatg aaatcaaaga acgtttgcaa atgaaggggt tgcgtgtaga agttgatgat 1740
cgtaatgaaa aaatgggcta taagatacgt gcctctcaaa cacaaaagat cccttaccaa 1800
ttagttgttg gagacaagga attggaagat gcaacagtga acgtacgtcg ttatggaagt 1860
aaagaaacag ctgtagaaga tttgaatatc tttattgatg ctatggaagc agaagtcaaa 1920
aattacagca gagaaaacta a 1941
Seq ID 354
atgactacta atttttggaa agagctgcca aagccttttt ttgttttagc accaatggaa 60
gatgtaacag atgttgcatt tcgccatgtc gtaaagcatg cagcagcacc ggatgtcttc 120
tttactgagt ttacaaactc agacagtttc tgtcatccag atggaaaaga cagtgtacga 180
ggaagattaa catttacaga agatgaacag ccgatggttg cccatatttg gggagacaag 240
ccagaatttt ttagagaaat gagcattgct atggcagaaa tgggcttcca aggaatcgat 300
atcaacatgg gctgcccagt ccctaatgta gcagaacgtg gaaaaggcag tggcttgatc 360
cttcgtccag aagtagcggc acaattgatt gaagcagcta aagctggcgg tttgcctgtc 420
agcgtcaaga cgcgtatcgg ctatacggaa atgtctgaga tggaagaatg gatcagtcac 480
ctacttcatc aagatatagc caatctttct gtccatttgc gtacgcgtaa agagatgagt 540
aaagtagatg cacattggga agtcattcct aaaatcattg ctttacgtga ccaaatcgct 600
ccacaaacac taatcacgat caatggagat atccccgatc gccagacagg agaagaattg 660
gccgagaaat atggcgttga tggcatcatg attggccgag ggattttcaa gaatccttat 720
gcatttgaaa aagacccgag ggaacataca gaaaaagaac tgatcggact acttcgtctg 780
caactagatc tgcaagatca ttattcagaa atcatcccgc gttccatcac aggactacat 840
cgtttcttta aaatctatat caaaggattt ccaggagcaa atgacttgcg agttcagttg 900
atgaatacga aatctactga tgaagtacga gagatcttag agacctttga aaaagaacat 960
ccaacgcttt tcagtgaaca atga 984
Seq ID 355
gtggtgaaaa agtcattcaa accgtttggg gagtgggcta taaaattgac gcaagatagt 60
aaacgagcaa ctgaaaaacg ccgtcggatc aatttgactt caaaagaaat cagcgaattg 120
ttagcggaag gaatcatcac gattatcctt ttgcttctat taaatgtttc tattctggtt 180
gttatcagtt cggtgatcaa cagttcacct tctttgacga atgcgatttg ggattcaaag 240
aatatttttg cagaacgatt gaacactgat ttgttctgga acggccggaa ttttattatt 300
ccctttttct ttctattaga tattggtgtt ttgtattggc gcttgattcg aagatatcgc 360
caaatgcaat tgcggcatat catcagcgaa cttcactaca tcgcaaacgg aaattatgat 420
catcgtatcc cttttgaact gagtggtgac ttaagccgag tggtaacaag tatcaatgga 480
ttggtggata gtaccgtagc agcgattgaa gacgaacgta aaatcgaaaa atcaaaagat 540
gaattgatca cgaatgtcag tcatgatatc cgtaccccac tcacgtccat cattggatac 600
ctaggactga tagaagatgg ccagtatcac tcagaagaag atcttttaaa gtatacgcat 660
actgcttata tcaaagcaaa gcagatgaaa tctttagtgg acgacttatt tgaatacact 720
aaggtacgcc agcctgctgt cccggtgaat ttttctgcct ttgacatgat ccagttgatc 780
gaacagttag cagctgattt tgagttggaa gcttctaaaa aaaatatcca aatacttgtt 840
caatcaaaag tagactccct tatcatggat ggcgataccg aaaaacttgt acgtgtcttc 900
aataatcttt tgacgaatgc gttgaaatac ggaaaaggag caacgaagat cgtaatcgaa 960
gtagaacgta tcggttcaga agttgttgca acagtcaaaa ataacggagc aatgatccca 1020
caacaggcaa tcgataatct attcgaccgt ttttatcgtg tagaagaatc tcgttcacaa 1080
gcaactggag gaactggact tggtttagca atcgctcaaa gcattgtggc tttgcacggc 1140
ggatacattt atgcaaaatc agataagcaa tggacttctt tcatcatgca ccttccaatc 1200
aagaaaaatg aaaaattacc tattgataca caggaagtta ttgacgaaag ctga 1254
Seq ID 356
atgaatcatt ttaaaggcaa acaattcaaa aaagacgtca ttattgtcgc tgttggttac 60
tacctgcgtt acaatctaag ctatcgtgaa gttcaggaat tgttatatga tcgtggaata 120
aatgtttgtc atactacgat ttatcgttgg gtacaagagt acagcaaagt cctctattat 180
ctttggaaga agaaaaatag acaatccttc tattcatgga aaatggacga aacctatatc 240
aaaattaagg gacgttggca ttatctttat cgtgcaattg atgcggacgg cttaacctta 300
gatatctggt tacgaaagaa acgggatacg caagcagcct atgatttctt aaaacgactc 360
cataaacagt ttggtgagcc gaaagcaatt gtgaccgata aagcaccttc tcttggctcc 420
gcctttagaa agttacagag tgtgggttta tatactaaga cagagcaccg aactgtgaag 480
tatcttaaca atttaataga acaagaccat cgacctatta aacgacgaaa taaattttat 540
caaagtctcc gtacagcctc ttccacgatt aagggcatgg agaccattcg aggaatatat 600
aaaaagaacc gaagaaatgg aacgctcttc ggcttttcgg tgtctactga aatcaaggta 660
ttaatgggaa taacagccta a 681
Seq ID 357
MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDLRAKLEEAVASFDNQDEVLFLVDLMGGTPFNQSNTLFE
EHKDKWAIVSGLNLPMLIEAYASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGDGK
IKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVKANVIPISKMIEVAKDPRFGNTKALL
LFENPEDVLTAVEGGVDIKELNVGSMAHSVGKVVVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKA
ELAKA
Seq ID 358
MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKVDDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNE
WFEDRMEEGAPPKNVNTMAPLLTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMMTV
LPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWARGNCWLTIAIPEIIEILELSKEDAL
RKLLIETLEAQVRALKTYQSESGLWHTLLDDPSSYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGE
VQNVSIGTGMGDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC
Seq ID 359
MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGLQANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAM
QLEKMLQDIQSLIKKDTEVLCLLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAAKK
LADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMVATIVNEFAKVAAVEKIELDVPEVIA
HCESCFDPETIGLHYPSMYQDLIKNHRLTEIDYINGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK
Seq ID 360
MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGTVAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMH
QKWPHVQQYIVYFQNFTNTHAPVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFEET
SKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDIQGIKLHLMHLMRKTRMLRDYHEGRL
QLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAPWDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV
Seq ID 361
MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINRGIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPA
NMLEVENCQGMIAVNGDSKEITDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFTGP
KTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAVMHDKQLKFTNLDQEGGEFVTKDISI
VLNTSFNNAEALKINYGDAYPERTSPDEEFPVDVIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVL
TGGAASLPGVVDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAARQMVVEQETTNTY
ESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD
Seq ID 362
MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDADNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLD
VINMQDYGMMNGEKVLAQAENLMK
Seq ID 363
MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKKKMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVT
QEFYAQRDKGASVDAAKQAVQSLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYEMI
KQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEGTPSVKKYIQSVTDGLYTWTTDQTKA
KHFITGHSYDIGNNDFAEASIEKGQLIVNHLEVGKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDK
TTPQLNGKDVAIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDGNKEIDPVNYSVTE
QTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQANVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQT
LAGAEFVVRDQDSDTAKYLSIDPSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTID
EQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQI
Seq ID 364
MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTITNWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIG
ADLNGVELAQVSRVLSTTSELNRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQNIR
RSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSASGQTLFIEPKQVVDLNNRLRQYQIAE
RNEIQRILSELSAELVPHRQEIIHNAYVIGKMDLMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIG
KDYQAIVITGPNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFSSHMTNTVDILSKV
NEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYPELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPG
PSNAFEISSRLGLDTEVIDEAKQLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREKE
MEKAKKKANEVVSEAEEKAEKIIADIPKMQQQIGQGNVKEHQLIDAKTQLANLHQEETLKKNKVLKKAKEQKTLKPGDEVL
VTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDELTPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYL
DSAILAGYPQVTIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK
Seq ID 365
MFDGYCSRVVNGNPNVKPATRKKVLEVIDRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNII
LANSDGNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSVNIDYVSATKDAVNKLAKS
GNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGLIFEAQYNFKDGLSLADRIHNSGATAVYVSDDELAIGILDGL
LDRGVKVPEGFEIITSNNSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK
Seq ID 366
MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIELTFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTA
RLAILAGAEYIVSPSFDQETAEMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMPTG
GVSLDHMKEWFDAGWTVGVGGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR
Seq ID 367
MDSVVVHRKSRLWEFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALENQQCDTPLAQQ
VLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFRIEPNVDEQQAERVLKLFEERKQEOAEAFMKOAA
ESLVVHEQKKKERKEQSPALEGPIHIQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTS
SFIVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMHAODIIEVKHTPRKDYAPEGEKRVELHVHSNMSTMDAT
NSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTG
LSAVYDTIIELAAVKMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTILVAHNAAFDM
GFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQHHRAIYDAEATGHLAWIFVKEAMDNHNMLY
HDQLNEHIGEGDSYKRARPFHVTILAKNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMM
QKGVEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGNVYHYLEEDAIYRKILINS
MGGANPLNRHSLPDVHFRTTDEMMLTAFHFLGEETAKEIWEHTHKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAK
QMYGDPLPEIIQKRLKKELNSINGHGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHYYCPECQ
YSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDIDLNFSGDYQAEAHNYTKVLFGEDYVYRAGT
IGTVADKTAYGYVKGYERDNNLQFRSAEVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHF
DFHSIHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSKTGTLGIPEFGTRFVRGM
LEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPD
ELRETYLSAMKENNVPDWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKGKDAVK
QAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIEGDTLIAPFRAVPSLGTNVAKQIVEARKD
GPFLSKEDLATRGKVSKTLIEYMNDNGVLKDLPDENQLSLFDML
Seq ID 368
MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGMRKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSI
RKDQPVYIYDQKKSLSIRTANKLRKAGYQEIYLLKGGYDGWSGKIKSKKV
MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKLVDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQ
AMRRLYPNIHFGVGPAIDSGFYYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELISEL
PEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYGTAFFDKKALKEYIRLREEAKERDHR
KLGKELDLFMVSPEVGSGLPFWLPKGATIRRTIERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHNDHYQEDMFPPMDMGD
GEMLVLRPMNCPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPDQIKDEFKRTLELM
VAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAMDELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLS
TIQLDFLLPERFDLTYVGEDGENTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIKE
RLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETAVEDLNIFIDAMEAEVKNYSREN
Seq ID 370
MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEFTNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKP
EFFREMSIAMAFMGFQGIDINMGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISHLL
HQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGEELAEKYGVDGIMIGRGIFKNPYAFE
KDPREHTEKELIGLLRLQLDLQDHYSEIIPPSITGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLF
SEQ
Seq ID 371
VVKKSFKPFGEWAIKLTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSILVVISSVINSSPSLTNAIWDSKN
IFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIRRYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLV
DSTVAAIEDERKIEKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLVDDLFEYTKVR
QPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDGDTEKLVRVFNNLLTNALKYGKGATKIVIEVERI
GSEVVATVKNNGAMIPQQAIDNLFDRFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEK
LPIDTQEVIDES
Seq ID 372
MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCHTTIYRWVQEYSKVLYYLWKKKNRQSFYSWKMDETYIK
IKGRWHYLYRAIDADGLTLDIWLRKKRDTQAAYDFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVKYL
NNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVSTEIKVLMGITA
EF0008
Seq ID 373
ATGATTAATAACGTTGTATTAGTCGGAAGATTGACAAAAGATCCTGACTTACGTTACACCGCTAGTGGTTCAGCTGTTGCG
ACCTTTACACTTGCTGTAAACCGTAACTTTACGAATCAAAATGGTGATCGTGAAGCAGACTTTATCAACTGTGTGATTTGG
CGTAAACCCGCAGAAACAATGGCTAATTATGCCCGTAAAGGTACATTATTAGGTGTTGTCGGAAGAATTCAAACTCGTAAC
TACGAGAACCAACAAGGTCAACGTGTCTACGTAACTGAAGTGGTTTGTGAAAACTTCCAATTGTTAGAATCTCGTTCTGCT
TCAGAACAAAGAGGAACTGGCGGCGGTAGCTTTAATAACAACGAAAATGGTTATCAATCACAAAATCGTAGCTTTGGTAAT
AACAATGCCAGTTCTGGATTTAATAACAACAACAATAGTTTCAATCCATCATCTTCTCAGTCGCAAAACAATAACGGTATG
CCTGATTTCGATAAAGATTCTGATCCATTTGGTGGCTCAGGTTCATCTATCGACATTTCAGATGATGATTTACCATTC
EF0028
Seq ID 374
ATGGCAGTAAAGAAAAAGAAAGCGACTTTCTGGGAGTTTTTCCAAGGATTAGGTAAAACGTTTATGTTGCCAGTGGCTTTA
TTAGCTTTTATGGGGATTTTGTTGGGATTAGGAAGTTCTTTTTCCAGTGAATCCATGATTGAAACGATCCCGTTTTTAGGA
AAGCCCGCAGTGAAAATTATTTTCCAATTTATGTCGACAATTGGTGGTTTTGCGTTTGCCTATTTACCTGTCATGTTTGCA
ATGGCCATTCCGTTGGGGTTAGTTCGGAAAGAAAAAGGAATTGCTGCGTTTTCAGGTTTCGTAGGCTATACGGTGATGAAT
TTAGCGATTAATTTCTATTTAGTGCAGACGAATCGCCTCGTGGATCCTGAGCAGTTGCGGGAAGCCGGCCAAGGAATGGTT
TTTGGGATTCAAACGATTGAAATGGGTGTTCTTGGTGGAATTATCGCTGGGCTTATTGTCTATAAGTTGCATAATCGATTT
TATACGGTGCAACTACCAGATAGTTTTGCCTTCTTTTCTGGCGCACGGTTTGTGCCAATTATTACTTCATTAGTAATGGCA
TTCGTGGGCTTAGTAATTCCGTTAGTTTGGCCACTTTTCGCGCTAATGATTATGGCGATTGGCCAACTTATCCAACGTTCT
GGTATTTTTGGGCCTTTTCTGTTTGGTTCAGGGGAACGTCTACTGTTACCGTTTGGGTTGCATCATATTTTGGTTTCCATG
ATTCGTTTTACTGAAGCAGGGGGTTCAGCGGTGGTGGCTGGCAAAGAAGTGTTTGGCGCGTTAAATATTTTCTATGCAGAA
TTGCAAAATAATTTACCGATTTCACCAAGCGCAACAGCTTTCTTGTCACAAGGGAAAATGCCGACCTTTATTTTTGGCTTA
CCAGCGGCGTCACTTGCGATGTATCATACTGCAGCTCCGGCGAATCGACATAAGATTAAAGGCTTACTCCTTTCGGGTGTA
ATTGCTACGGCTATCACTGGCATTACAGAACCAATTGAATTTTTATTCTTATTTATTAGTCCATTATTATGGCTGTTTCAT
GTCATTATGACTGGTCTAGGTTTCATGGTAATGGCTTTATTAGGTGTTGTGATTGGGAACACTGATGGCGGGCTACTGGAC
TTTGTTATTTTTGGCTTGTTACAAGGAACTTATACAAAATGGTGGTGGGTTCTCATTGTCGGCGCTATCTGGTTTGTTGTT
TATTATTTCGTCTTTAAAACAGTCATTGTAACCTTTGATTTAAAAACACCTGGACGAGACAAGGTCTTAGATGAGACTGAA
TATACCGATCAAGAAGTTCAATATAAGAAAACGGGTGGCTATGATGCGCCTGGGATTTTAGCGGCTTTGGGTGGGCAAGAG
AATATTCAAGCTATTGATAATTGCATTACGCGTTTACGTTTAGTGTTAGCAGATGCTAATAAAGTAGATGATGACAAATTA
AAGCAATTAGGGGCTTTAGGCGTTGTACATTTAGATGCGCAGAATGTACAAGTGATTATCGGAACCAAAGTGACAACGGTC
CGTAATCAATTAGAGATGATTTTAGGT
EF0146
Seq ID 375
ATGAAAAAGATCGCAAGTGCAGGGTTAAGTATTTTAGTCGCAACGGGGGTAGCAGGTATTGGGGGAAATGAAGTACAGGCA
GCAGAACAAGCGCAACCAAAAACACCTGAAAACAGTTCTACAGAACAACCAGCAGTGAAAGCTACAGAAACAACGGAGCAA
GCCATTACTGAAAAACAGCAACAAGTAACAGAGAAACAAGCAATTGTCGATCAAAAACAACAAGTTGCTGACACTGCGAAA
AAAGAAAAAGACACCATTGATCAATCTGTTAAAGACCAACAAGCAGTGGTCGATCAAAACAAAGGTGCATTGGATCAAAGT
CAACAAGCAGTGACTGACCAACAAGCGGTCGTAGACGAAGCAAAAAAAGTTGTGGATGAAGCAACACCTTCAGCCATTGAA
AAAGCCAAAAACCAAGTGGCTACGGATACACAAGCTGTTGATGACCAACAAAAAGTAGTGGATCAAGCTCAAGCAGACGTT
AACCAACAACAAGCAGTTGTCGAAGAAAAAGCAAAAGAAACGAATGCTGCTAAAGTGCAAAATGATAAAGATCAACAAGCA
GTAACAGCTGCGAAACAAGAACAAGCCAAGCTTGAAGAATTAGCGAAAAATGCGGAAGTGGAAAAAGCAAAGGCTGAAAAA
GAACAAGCAGCAAAAGAAGCTGAACTGGCTAACAAACAGAAAGAAGAAGCCAAAGCTAAAGATCAAAAAACGAAGGACGAT
CAAGCAGTCGCAGACCAACAAACTGTTGTGACAACTAGTCAAGAGAAAGTAGCAGACGCAAAAGCAGATACAGCTGCGAAA
CAGGCAGACTTAACAGCGAAAGAGAATGCTTTGAAAGACAAGCAAACAGCAACAAAGCAAGTGCAGAATACTTTAGATAAA
TCAAAAGAAGAGCTAAAAGGACATAAAGGAATTAACTTGCCTGCTAACTTCACACCTGATTATTACAAAAAATTATCTGAA
CAAGAAAAGCAAGCAATGGAAAAAGAAGCATTAGCATTAAATAAAGTTTTTCCTGAAAATCAAGCAGATGTGGCAAAAGCA
ACGGAAATGATCAATGTCAAAAATCCTACCGGAAAACAAAAGCAACAAATGAGCGATTACGTTGTAGGACTTATCAATGAT
GTTCGCGAAAAGCTTGGGTTACAAAAGTTGAAGATTTCTAACCAAGCTATGAGATTTGCTTGGGATGTAGCAAAATATGAT
AATCCCAAAGAGTTTGATCATGACGTAAATGCAATTAATCGTGCAGCAAAAGAAAATGGTTTTAAAGAGTATCCAGGGCAA
AACTTTTATGAAAATCTAAGTATGGGCTATTTTGAAACGATTAATGGCACTATTTCTCAACTAGAGTTTGAAAAAGCTGCT
CGAAAAACAATTGCTGATATGCTCTTTGACGATGAAAGTTCAGCGTATTCTCATATAGATTCATTGCTAAAAGGGGACACA
ACAAACATGGCAGTTTCTATTTCAGGAGATTTAAATGATATTTCGGCAAAAATCCATATCATTAGCTATAATCAATCAAAA
TTAGTTGAAGCAAATACCTATGAAGAAGGCACTGCTCCAGTCTTTAAGAGTAAAGAAACCCTTCAAAAAGAAGTAGCAACC
AATCAAGAAAAATTAGCTACTGCACAACAAGCAGAATCAGACGCTCAACAAGCAAAAAGTGCAAGTCAGCAAGTCTTAAAT
ACAGCCAAAACAACACAAGCAACAGCAGAAAAAGAACTATCTGTTCATAAAGCGACATTGGCTAGTCTTCAAGCAGTTGCG
ACTAAAAGTACGACAAATTATGAAGAAAAAGTACGACAAACTGCAACGGCAGAAAAAAGCCTTCAACAAACAAAAGATCAA
TTGGCAACAATCAATGAGTTAATTCAGAATCGAGCTGCTGTTTTAGAAAAAGCGAAAACAAACGTTTCGGAAGCACAAGCA
ATTGAACAAACGTCTGCTAAAGTGCTGAAAGAAAAACAAGCAGCCCAAAAAGCAGAAGAAAACACATTGAATAGCTTGAAG
GAAGTATTGAATTTAGCAAAAGAAAATTTAAATCAAAAACAAGTTGCATTTAAAACAAGTACACGTTCATTGTCTCGTTTA
GAAAATGCTCAACCAACATACGAAAAAGCATTAAACGAGTTAAACAAAGCAGAAGCAGCAGCGGTCCAAGCACAAGAAGCC
TATGAAAATTCTCTGAAATCATTGGAGGAACTTAAAGAACAACAAGCCGTTGCTACACTTGCTTATACACAAGCACAAGAA
GACCTTTCTAACGCGAAGTTAGAGCTACAGCAGTACCAGGGCGTATTAAGAGAATTAGAAGCACAACAAGCCGAACAGCAG
CGACAAGAAGCGTTGCAAGAACAAGTAGCAAAAGAACAACAACGCCTTGAACGAGAAGCAAAACAAAGCCAAACGTTAGTA
GCAAGTGCTACTTCAGCAGACAAAACACCTGGTCTCCAACAGTTATCTTTTTCTAAACAAAAAGAACAGCCAAAAGCACAA
GCATTAACACATTCAGAATCTCGTAAGACGAAACAAGTAGCAAAAGCCCCAGATTCTTTACCACATACAGGAGAAAAAAAT
AATAAATGGTTAGCTATAGCTGGTCTGATATTTGCTTTGTTAGGAGCTGCGGGTATTATAAGTTTTATTAGTAGAAACGAG
AAGAAAGTAAAAAATATCTTTAAAATTAAA
EF0153
Seq ID 376
ATGAAAAAAATGATTATTATTGCCTTATTCAGTACAAGCCTTTTAGCAGGGGGAAGCAGTGTTTCTGCTTATGCGCAAGAA
TCAGAAGGAAATCTTGGTGAAACAACAGGGAGTGTTTTACCAGATGAACCGAATGTACCAACTGACCCAATAACGCCAAGT
GAGCCAGAGCAACCAACAGAGCCAAGTACACCAGAGCAACCATCGGAACCGTCAACACCAACCGAACCTAGTGAGCCTTCA
AAACCGACGGATCCTTCGTTACCAGACGAACCGAGCGTACCAACAGAGCCAACAACGCCAAGTAAGCCAGAGCAACCAACA
GAGCCAACAACGCCAAGTGTACCAGAGCAACCAACAGAGCCAAGTGTACCAGAAAAACCAGTAGAACCAAATAAACCAACC
GAGCCAGAAAAGCCTGTGCCAGTTGTTCCTGAAAAACCAGTTGTACCACAACAACCAGAGCAACCAACAGATGTGGTGGTA
AAGCCAAATGGAGAAATTGCAACAGGAGAATCTACACAACAGCCAACTGTTCCAATTGAAACGAATAACCTTTCAGAAGTA
ACACATGTCCCAACTGTGACGACACCGATTGAAACAGCAAGCGGAGAAGCAATTGTCGCAGTGGATAAGGGCGTTCCTTTA
ACACAAACGGCTGATGGATTAAAACCGATTAAAAGTGAATATAAAGTATTACCAAGTGGCAATGTACAAGTGAAAAGTGCT
GACGGAAAAATGAAAGTACTTCCTTACACTGGTGAAAAAATGGGCATAATTGGGTCAATCGCTGGTGTATGTTTGACTGTT
TTATCAGGAATCTTAATTTATAAAAAACGTAAAGTG
EF0394
Seq ID 377
GTGAAGAAACGTCTATTTGCATCAGTATTACTATGTTCATTAACGCTATCAGCAATTGCTACCCCAAGCATCGCTTTGGCG
GACAATGTTGATAAAAAAATTGAAGAAAAAAATCAAGAAATTTCATCATTAAAAGCAAAACAAGGGGATTTAGCTTCACAA
GTATCTTCTTTAGAAGCAGAAGTATCTTCAGTATTTGATGAAAGCATGGCTTTACGTGAACAAAAGCAAACACTAAAAGCA
AAATCAGAACAATTACAACAAGAAATTACAAACTTGAATCAACGTATTGAAAAACGTAACGAAGCAATCAAAAATCAAGCA
CGTGATGTTCAAGTTAATGGACAAAGCACAACAATGCTAGATGCAGTTTTAGATGCGGACTCAGTTGCAGATGCAATCAGC
CGTGTTCAAGCTGTTTCAACAATCGTAAGTGCCAACAACGACTTAATGCAACAACAAAAAGAAGACAAACAAGCCGTTGTT
GATAAAAAAGCTGAAAACGAGAAAAAAATGAAACAACTTGAAGCAACAGAAGCTGAATTAGAAACAAAACGTCAAGATTTA
CTTTCTAAACAATCTGAATTAAACGTAATGAAAGCTTCATTAGCATTAGAACAATCATCAGCTGAAAGTTCTAAAGCTGGC
TTAGAAAAACAAAAAGCAGCTGCTGAAGCAGAGCAAGCACGCTTAGCTGCTGAACAAAAAGCTGCAGCTGAAAAAGCCAAA
CAAGCTGCTGCAAAACCAGCTAAAGCTGAAGTGAAAGCAGAAGCACCAGTTGCCTCTTCATCAACAACAGAAGCACAAGCA
CCAGCAAGCTCAAGCTCAGCAACTGAATCAAGCACGCAACAAACAACTGAAACAACTACACCAAGTACAGATAATAGTGCA
ACAGAAAATACTGGCTCTTCTTCATCAGAACAACCAGTACAACCTACAACACCAAGCGATAATGGAAATAATGGTGGCCAA
ACTGGTGGTGGAACAGTTACACCAACACCAGAACCAACACCAGCGCCTTCTGCTGATCCAACAATCAATGCATTGAACGTT
CTACGTCAATCATTAGGTTTACGTCCAGTAGTATGGGATGCAGGTTTGGCAGCTTCTGCAACTGCTCGTGCAGCACAAGTT
GAAGCAGGTGGCATTCCAAATGATCACTGGTCTCGTGGAGATGAAGTTATCGCAATTATGTGGGCGCCAGGTAACTCAGTA
ATCATGGCGTGGTACAATGAAACAAACATGGTAACAGCTTCAGGAAGCGGTCACCGTGATTGGGAAATTAACCCAGGTATT
ACGCGTGTCGGTTTTGGTTACTCAGGTAGCACAATCGTAGGACACTCAGCC
EF0443
Seq ID 378
ATGAAATCAATCAAAACGATTTTACTTGGAACAACTTTGGCCGCAGGTTTAGGATTATTCTTAGGAACAGACGCAAACGCA
GAAAGCTTATATACAGTTAAAGCAGGAGATACTTTATCAACAATTTCTCATCAATTTGCAGGAGACAATAGCTTAATTCAA
AAAATTGCTTCTGATAACAAATTGCCAAACCTTGATTTAATTTTTGAAGGAGAACAATTAGTTATTCGTTCAGAAAAAGAA
GTTGCTAATACTCCAGCACCAGCTGTAGAAGTTGCACCAGTTCAACAAGTAGTTGAACAACCTGTTGCACAACCAGTACAA
CAAGAAGTACAACAACCTGTTGCTCAAGAAGTAGCGCAACCAGCAGCACCTGCTGCAAGCAGTGATGCAAAAGAGTGGATC
GCACAACGTGAATCTAGTGGTTCTTACGATGCAACAAACGGTCAGTATATCGGTCGTTACCAATTATCTGCCTCTTATTTA
AATGGTGACTATTCACCTGCCAACCAAGAACGCGTGGCAGATCAGTACGTAGCAGGTCGCTATGGTTCATGGGATGCAGCT
AAATCATTCTGGTTAGCAAATGGTTGGTAC
EF0568
Seq ID 379
ATGAAAAAAATCTATCAATGGGCAGTGGGACAATCCTTTAAAAAATTGGATCCACGCCAACAAGTTAAAAATCCCGTGATG
TTTGTCGTTTATTTAGGTGCGCTCATCACTACAATATTATGCTTCTACCCAATGGGTATACCGTTATGGTTTAACATTTCA
ATAACCATTTTTCTTTGGTTGACTTTGCTTTTTGCGAATTTTGCTGAAGCTGTTGCAGAAGGACGAGGAAAAGCACAGGCC
GATAGTTTGAAACAAGCAAAAAAGGAAGTCATGACTTATAAAATTAACAGTTTAGAAGATATCAAAGAAGAGAATTTCATT
GAATTACAGTCTTCTGATTTAAAGCGAAACGATTTGGTCTATGTACGTGCAGGAGAGCAAATCCCAGCTGATGGTGATGTA
ATCGAGGGGGCTGCATCAGTAGATGAAAGTGCCATTACTGGCGAATCAGCGCCAGTAATTCGTGAATCTGGTGGGGATCGC
AGTGCGGTTACTGGCGGAACAACCGTCGTTTCCGATTATTTAGTGATTCGTGTTACCTCGGAAAACGGTCAATCCTTCTTA
GATAAAATGATTGCCATGGTAGAGGGAACTCAACGAAAAAAGACTCCGAATGAGATTGGTTTGCAGATTTTCTTGATTACT
TTGACCATTATTTTCTTAACTGTATCAATTACTTTAGTCCCATTTACTGATTTCAGCAGTCAGTTATCTGGTAAAGGAGAA
GCTTTATCACTGGTTATTGTGATTGCTTTACTAATCTGTCTGGCGCCAACGACGATTGGGGCCTTAATCTCTTCGATCGGG
ATTGCAGGTATGAGCCGCTTGACGAAGGAAAACGTCATTGCCATGAGTGGTCGTGCGATCGAAGCAGCTGGTGACGTGGAT
GTTCTTTTACTCGACAAAACAGGAACCATAACATTGGGAAATCGACGAGCAAGCGATTTTTTGCCAGTCCATGGTGTTAGT
GAAGAACAGTTAGCGGATGCGGCGCAATTATCTTCTTTAGCTGATGAGACAGCAGAAGGTCGAAGTATCGTGATTTTAGCA
AAAGAACGTTTCAACTTGCGGGAAAGAGAATTCCAGCAATCAGAGGTTAAATTTATCGATTTCAGTGCCAAAACACGCATG
AGCCGAATTGATTATCGCGGTGACGTGATTCGCAAAGGTGCCGCAGACACCATGAAAAAATATGTACAATCTAAAGGAGAG
GACTATCCATCTGAATGTGACAAGATTGTTGATAAAATCGCTCGAGCAGGTGGAACACCTCTAGTAGTTATAAAAAATAAT
CGTGTGATGGGTGTCGTCTATTTGAAAGACATCGTTAAGAATGGTGTAAAGGAAAAATTTGCTGATATGCGTAAGATGGGT
ATCAAGACCATCATGATCACTGGGGATAACCCCTTAACAGCGGCAGCGATAGCAGCGGAAGCTGGAGTAGATGATTTCTTG
GCAGAAGCTACCCCAGAAAATAAAATGAATCTGATTCGCGAGTATCAAGAAAAGGGTCACTTAGTTGCAATGACTGGTGAT
GGGACAAACGATGCACCAGCATTAGCGCAAGCCGACGTTGCGATGGCGATGAATACAGGAACACAAGCCGCGAAGGAAGCG
GGGAACATGATTGATTTGGATTCCAGTCCAACAAAATTGCTGCAAGTCGTACAGATTGGGAAACAATTGTTGATGACTCGC
GGCGCGTTAACTACGTTTAGTATCGCAAATGATATCGCTAAGTATTTTGCGGTGATTCCAGTACTATTTTACAGTATTTAT
CCGCAACTGGATCCATTGAATATTATGGGATTAGGTAGTCCACTGACTGCGATTCTATCCGCTGTCATTTATAATGCAGTC
GTTATCGTGGCCTTGATTCCACTAGCATTAAAAGGTGTGAGGTATCAAGAAAAACCGGCCAGTCAAATTTTGAGTCATAAT
CTGCTGATTTATGGACTTGGGGGAATTATTGCACCATTTATTTTTATCAAGATTATAGATCTGATTCTCTCGCTGATCATT
TTA
EF0591
Seq ID 380
ATGTTTTTTACTGGTAAAGAAAGGAATTTGAAATTTGCTATTAAGAAGAAACACGGTGGCGGTGGTGCAGCATCAGTAATT
ATCGGATGTATTATTTTCGGAAGTATTGCGTTTGGTGGGACAACATTAGCTTACGCTGATGAAGTGCATAATAGTATAAAT
CAGGATATACAAGATTCTGGTAGTACAATTATTGGAGAAAATGATTCTTCTACCAAATCAGCTGAGTATAAAATGATTCAT
GAAATTGATGGAACTAAAATTAGTAACGGTGAAAATAGTAAAGAAACAACTACAAGTTCAGGAACTATACTGGCTGAAGAA
GCAATAGAAAGTTCAAATCAAAAAAATTCAAAGACAAGTGAAGTCGAACAGGATCTTCATAAAGATGTATCAGGATCTCAA
TCAGTAAAACAAGTAGAAACTTCTGATTCTATAAAAAAATCTCAAGAATCAGCTGTTAAAACATTAAATCTGGATGATTCA
CAAGAGAATACTAATTCAATAACTACCAAGGCAGAAAATGATGCGCTATCTACAGTTAATGATGAAAAAGTATTAAATGAA
AGTGATAGTATTATCAAATCAATTCCTTCGGAAACAGAGAATGTCGATAATAATAGTAAAAGTGATAATAGGGACTTAGTA
GAAAACTCAGTGGAAACTACTAAGGAAATAATGAAAGAGGCTGATAAAACTGAAATAGCTAAAAGGAATGACAATATCTCT
GTATTGAATGCTGGAACTGAATCATTTAATGATAGTGATAAAATAACAAGATCTAAAAGAGATAATGAATCTCCTGTTCCA
AGTTTGAGTATTCAAATGCCAGTCACAAAAGAGATTTACGAAAATGATAGTACTATTAAAGGAAAGTCTGAACCAGGAGTA
ACGATAAAAATTTATAAAAATGGTATTGAACTGGGGTCTATTAAAGCAGACGATGATGGAAATTTTGAATATCCTCTAGAA
TCACAAGCTATCAAAAGTGATAACTACTCTTTCGTTGCGGAAAAAGATGGAGCGAAAAGTATAGAGAGCTCTGTAAATGTA
GTAGAAAATAAAGAAATAGTAGGTAATCAACGAGATGCGTATACTCCATATGGGTTTGGTATATATAATCGAGGTTTAGTT
ACTACTGATGATGGAAAGAAACATTATACAGTTGATATGGAGGGGAATTTAGCATCTGAAGCGTACTTTGATCTTGCAGGC
GGAACAATGTACTATTCAATTGATGAAAAATTTGCACCATATGTTGAAAAAATAGTTCTAGATGGTAAAACAATTATTTCT
AAAAATCCGTATGAATTGCCCAATAAAACCAATATTTGGTCTTCTGGTATTCTTAGTACAGATAAAAGAGGAGGATTGGTT
CGTGCTGCTTTAGTGGGTCGTACTAACGGAACAATAGATATTTATTTTAAAGATGATACTCCTCAAGAAGTTCTCAATAGT
GAAATACCTTTCCAAGTTTGGGCTAGATTTAAAGAGAAAAAAGAGGGAGTAAAAAATGATCTAATTTCTGATTTTGATCTA
AATATTTTACTTCAAAACAATAAATTAATATTAAGTAAAGATACCTTTTTCAAAGATGAAGATATTGTCAATAATAATGGT
CCTAAAATTAGTTCTAATTTTAACTATACCAATAATACTATTGATATTAATTATGGAGTAAATACTGGTAATTATGGCTTA
ACGATAGGGAAACGTAGTCCTTATAACTTGCATATTAATACAGGGAAAGAATTAAGTGATTTAGTTGATACGATAAAGATT
GACAATAAAAATTATGAGTTCGAAAAACTTCCTGATGGTTCGTTAGTGATTAAAGATATATACAAAAAGGGAATTTTAGGT
GGAGCATTACTTAACCGCCAAGAGATTACAATCAGTTTAGGATTAATAGCGAAAAAAAATCTGGGCGATCTCATTGATTTC
AAACAAGAATTATTAAAACTTGATGTATCTATTAGAGATAATACAACCTTTAAGCTAGTAAAAGGAGAAGCTACAGAAGCT
ATCCGTAAAATAGATTCCTCATTCGATACATTTAAACAGGAATTAACGAAATGGATTGAAAAAAGAGATAGTAATCCACAG
GAACCTATAACTATTAATTCTGGAGACAATACTTCTATAGTGGATCCTAAAGAGATGGTTGAATTTGCAGTAAAAAATCCA
ACCACAGAAAATATTAATAAAGCACGTAGTTTGGTTTCTAAAATGTGGCTTTGGGACAAAAACAAATATAATCCAGTTCTT
AATGCTGCTGAAGAAGTAAACAGATATCTTTCAACTACGTTAAATGGTTCATCAAATGATGATGGCTTGATATTAAATAAT
ACTCATGCGGCAACTTTCGTTAAATTAATTGATACGGATAAAGATGGGATACTTGATAGATACGAAGCAAACATGGGAATT
CGAACCAACTACACAAATGCTGATTCTGATGGTGATGGAAAAAGTGACGGATTTGAAATTTTTAATGATACTGATCCTTTG
GTGAGTCCGTACGATTGGTTTGATAAAAATGGAGAAAAAATAAAAATAGTAACAACCGATACTGATACGATAAGTGGAAGA
ATAGGAAATAACAACTATGATACTGAAAATGTCTACCCTAGAACTGTTCAATTAATAAAGGTTACTGATAACGGAGAAAAT
CTGATTTCTGAAATTAGTTCTTCTGAAGATAGAAAAGGGACTTTTGAATTTACTGGATTGAGTGGTAAATTAAGCAAGGGA
GATAGACTCGTTGTTAAAATTATTACTAATGAAGTACAAAGAGAAATTGATCGACAAAGAAATGTTGTTCAAGTTGGTTAT
GATAATCCAGAGGTATCTGAAGAAGTAATTGTTCAGGGCGCACAAGTAACAGTCTCTTTTGACTTTAATTATAATCAAACT
GATCCTGACTTTGACCGTCAACCAGTTACTCAAGTAGTAGAATTAGAAAAAGGTTCAAGGCTTTTAAATAGTTATTTTACT
CCAGAACGAAAAGGCTATAAATTTATTGGATGGAATACTGATAAACTTGGAGTAGGACAAACTGTCAATGATGGTTCTAGT
TTTAATGAAGATATAACGGTATTTGCTCAATGGGAAAAAGAACCTAACCTAGCTGGAGAAGTACATGCTCCACAAGGTCCT
ATTGAAGAAGGTAAAAGTATTGTTCTAAATAATGTAGTACAGTCCAATAAATCAGGGTCTGTAATTGTAACTGGAGAACTT
CCAAAAGGGCTCTTTGGGTTATCTATAAATGAAACAGGTAATCTAATTGGTACTCCACTTATCAATGACTGGATTGATGGA
GAAAATAGTCGGGAAGTAAAGATTCCAGTTACAATTAGTAATGGTGACGAAAAGGTTATGGTGGAAGTTCCATTAACGATC
CTGCATGATACTCGAGCGGCAGACGCAGTAAAAGCAGCAGAAGACGCAGGCAAAGCTGGAGCAGATAAGAAAGCCGAAGTG
GAAACCGACGGTTTAGTGACTCCAGAGGAAAAAGCGGCAGTGGATGGCTTGAATGACACGACTACCGCGAAGAAAGAAGAC
GCAAGCAAGTTAGTGGATGCCTTGCCAGAAGGACCAGTAAAAGATAGCTTGAAAGATCGCTTAGATAAAGTGACAACCTCT
GGAGTAACAGTGAATGATGCAGATAGCAATGGCAAAGCGGACGATGTAGATTTAGCTGAAAAAGCAGCGGCAGACGCAGTA
AAAGCAGCAGAAGACGCAGGTAAAGCTGGAGCAGATAAGAAAGCCGAAGTGGAAACCGACGGTTTAGTGACTCCAGAGGAA
AAAGCGGCAGTGGATGGCTTGAATGACACGACTACCGCGAAGAAAGAAGACGCAAGCAAGTTAGTGGATGCCTTGCCAGAA
GGACCAGTAAAAGATAGCTTGAAAGATCGCTTAGATAAAGTGACAACCTCTGAAGTAACAGTGAATGATGCAGATAGCAAT
GGCAAAGCGGACGATGTAGATTTAGCTGAAAAAGCAGCGGCAGACGCAGTAAAAGCAGCAGAAGACGCAGGTAAAGCTGGA
GCAGATAAGAAAGCCGAAGTGGAAACCGACGGTTTAGTGACTCCAGAGGAAAAAGCGGCAGTGGATGGCTTGAATGACACG
ACTACCGCGAAGAAAGAAGACGCAAGCAAGTTAGTGGATGCCTTGCCAGAAGGACCAGTAAAAGATAGCTTGAAAGATCGC
TTAGATAAAGTGACAACCTCTGAAGTAACAGTGAATGATGCAGATAGCAATGGCAAAGCGGACGATGTAGATTTAGCTGAA
AAAGCAGCGGCAGACGCAGTAAAAGCAGCAGAAGACGCAGGTAAAGCTGGAGCAGATAAGAAAGCCGAAGTGGAAACCGAC
GGTTTAGTGACTCCAGAGGAAAAAGCGGCAGTGGATGGCTTGAATGACACGACTACCGCGAAGAAAGAAGACGCAAGCAAG
TTAGTGGATGCCTTGCCAGAAGGACCAGTAAAAGATAGCTTGAAAGATCGCTTAGATAAAGTGACAACCTCTGAAGTAACA
GTGAATGATGCAGATAGCAATGGCAAAGCGGACGATGTAGATTTAGCTGAAAAAGCAGCGGCAGACGCAGTAAAAGCAGCA
GAAGACGCAGGCAAAGCTGGAGCAGATAAGAAAGCCGAAGTGGAAACCGACGGTTTAGTGACTCCAGAGGAAAAAGCGGCA
GTGATGGCT
EF0592
Seq ID 381
TTGCCAGAAGGACCAGTAAAAGATAGCTTGAAAGATCGCTTAGATAAAGTGACAACCTCTGAAGTAACAGTGAATGATGCA
GATAGCAATGGCAAAGCGGACGATGTAGATTTAGCTGAAAAAGCAGCGGCAGACGCAGTAAAAGCAGCAGAAGACGCAGGY
AAAGCTGGAGCAGATAAGAAAGCCGAAGTGGAAACCGACGGTTTAGTGACTCCAGAGGAAAAAGCGGCAGTGGATGGCTTG
AATGACACGACTACCGCGAAGAAAGAAGACGCAAGCAAGTTAGTGGATGCCTTGCCAGAAGGACCAGTAAAAGATAGCTTG
AAAGATCGCTTAGATAAAGTGACAACCTCTGAAGTAACAGTGAATGATGCAGATAGCAATGGCAAAGCGGACGATGTAGAT
TTAGCTGAAAAAGCAGCGGCAGACGCAGTAAAAGCAGCAGAAGACGCAGGTAAAGCTGGAGCAGATAAGAAAGCCGAAGTG
GAAACCGACGGTTTAGTGACTCCAGAGGAAAAAGCGGCAGTGGATGGCTTGAATGACACGACTACCGCGAAGAAAGAAGAC
GCAAGCAAGTTAGTGGATGCCTTGCCAGAAGGACCAGTAAAAGATAGCTTGAAAGATCGCTTAGATAAAGTGACAACCTCT
GAAGTAACAGTGAATGATGCAGATAGCAATGGCAAAGCGGACGATGTAGATTTAGCTGAAAAAGCAGCGGCAGACGCAGTA
AAAGCAGCAGAAGACGCAGGCAAAGCTGGAGCAGATAAGAAAGCCGAAGTGGAAACCGACGGTTTAGTGACTCCAGAGGAA
AAAGCGGCAGTGGATGGCTTGAATGACACGACTACCGCGAAGAAAGAAGACGCAAGCAAGTTAGTGGATGCCTTGCCAGAA
GGACCAGTAAAAGATAGCTTGAAAGATCGCTTAGATAAAGTGACAACCTCTGAAGTAACAGTGAATGATGCAGATAGCAAT
GGCAAAGCGGACGATGTAGATTTAGCTGAAAAAGCAGCGGCAGACGCAGTAAAAGCAGCAGAAGACGCAGGCAAAGCTGGA
GCAGATAAGAAAGCCGAAGTGGAAACCGACGGTTTAGTGACTCCAGAGGAAAAAGCGGCAGTGGATGGCTTGAATGACACG
ACTACCGCGAAGAAAGAAGACGCAAGCAAGTTAGTGGATGCCTTGCCAGAAGGACCAGTAAAAGATAGCTTGAAAGATCGC
TTAGATAAAGTGACAACCTCTGAAGTAACAGTGAATGATGCAGATAGCAATGGCAAAGCGGACGATGTAGATTTAGCTGAA
AAAGCAGCGGCAGACGCAGTAAAAGCAGCAGAAGACGCAGGTAAAGCTGGAGCAGATAAGAAAGCCGAAGTGGAAACCGAC
GGTTTAGTGACTCCAGAGGAAAAAGCGGCAGTGGATGGCTTGTTAGAAATAAAACAGTCTTCATTTATGCCGTTTGAAAAT
TTATTTTCGACTACAAATGATTACTCACAGTTTCCTAAAACTGGTGAAAAATCTGATTCTATTTTAACCATTTATGGAGGT
TTATTATTCTTAAGTAGTATAGGATTATTAGGAATAAAAAAAAGAAAAAATAATACGAAT
EF0658
Seq ID 382
ATGAAGCGAAAAATTAATTATACACATATTGCTGAACCTACTGTTTTTGAGAATGTTTTTCCAACGTATTTGACGAATGAA
ACGATGATGGCGCGCAAGCAGAAAGTATTACAACGAATGGAAACAGAAAAATTCGATCAATTGGTCTTCTATGCAGACAAA
GAGCATGGCAGTAATTTTGAATACTTAACTGGCTTTATTCCGAGGTTTGAAGAAGGACTTCTTGTGTTAAATAAGGATGGT
GCGGCGACACTTATTTTAGGTAATGAAAATTTAAAACTGTGCCAACATGCCCGCATCTCAGCCGATTTAATTCATTATCTA
GCTTTTTCACTACCTAATCAACCAATGGCTGGGGAACAAAAGTTGTCGCAGATTTTTGAAACGCTTCTCGATGAAACTGCT
CAAAAAATAGGTATCGTTGGGTGGAAAATGTTTACGACACAACAGCAAGAACCTGCTACTATGTTTGATGTGCCACATTTT
ATTGTCGAAGCACTGAAAAAAGCGCTGCCGAAAGAAGCTCGTTTACTAAATGGGACCCACTTATTGATTGGAGCAAAGGGG
GCTCGCGCAACAAATAATGCTAACGAATTGGCGCACTATGAATATGGGGCGAATTTGGCTTCTCGCAGTATGTTAAAGGCC
TTAAATGCAATTGAAATAGGACAGCGTGAAACGGACATTGGGGCTTTGTTAAATGATGAAGGTCAAACGCCGACGGTAGTA
ACCATTGCGGCAACTGGTCAACGTTTTGAATATGCAAACATGTATCCGACTGCCAAAGAAATTCAGTTAGGTGATGCCTTA
TCATTAACTACAGGCTACAAAGGCGGCTTGTCTAGCCGAACAGGTTTTGTTATTGAAAACGAGCAGCAATTGCCAGAAGCG
CAACGTGATTATTTAGAGCGCGTAGCAAAACCCTATTTTCAAGCTGTCGTTCACTGGCTTGAAACCATTCGAATCGGACTG
TTAGGGCGAGAAATGTACCAAGCGATTGAAGAACAGTTACCAAAAGAAATCTATCACTGGCATTTGAATCCTGGGCATTTA
GTTTCGGATGATGAATGGATGTCGTCGCCTATTTATCCAGATTCAGCCATTCGCTTGGAAAGTGGCATGCTCTTTCAAGTG
GATATTATTCCTTCTGTACCTGGTTATACAGGGGTGAGTGCAGAAGAATGTGTGGCTTTGGCTGATGAAACACTGCAAAAA
GAGATTCAGCAAACTTATCCAGACATGTGGCAACGGATTGCGACACGCAAAGCCTATTTAAAGGAGACATTAAAGCTCGAT
TTACCATCGGAGGTTTTACCAATGTCTAATTTAGTTGGTTACTTACGGCCTTTTTATTTAGCTAAAGACAAAGCTTTGTGT
GTAGAAAAACCAGCGCTTAAA
EF0727
Seq ID 383
ATGAAAAAAGCTAGAGTGATTTATAATCCAACGTCAGGAAAAGAGTTAATCAAAAAGAACTTAGCCGATATTTTATCTATT
TTAGAAGAATGTGGTTATGAAGCCAGTGCATTTGCGACCACACCAGAAGAAAATTCAGCACGCAATGAAGCACATCGTGCT
GCGCGGGCAGGATTTGATTTACTAGTAGCTGCAGGTGGAGATGGGACCATTAATGAAGTCGTGAATGGGATTGCTCCGTTG
AAGCGGCGCCCTAAAATGGCTATTATTCCTGCTGGAACGACGAATGACTATGCACGGGCCTTGAAGATTCCTCGTGATAAT
ATCGTTAAGGCAGCAGAAGTGATTAAAAAAAATCAAACTGTCAAAATGGATATTGGCCAAGCCGGCAAAAATTACTTTATC
AATATTGCGGCGGGTGGTCATTTAACGGAACTGACTTATGAAGTTCCGTCAGAGTTGAAAAGTATTTTTGGTTACTTAGCG
TACTTAGCCAAAGGAGCCGAAATGTTGCCGCGAGTGAAGCCGATTAAAATGCGCATGACGTATGATGAAGGTGTGTACGAA
GGCAATGCATCAATGTTTTTCCTAGGGCTGACTAACTCGGTGGGTGGTTTTGAGCAAATCGTACCAGATGCTAAATTAGAC
GATGGTAAGTTTTCATTAATCATTGTAAAAACAGCCAATATTTTTGAGATTCTTCATTTAGTTGCGTTAATGTTAAATGGT
GGAAAGCATGTTGAAGATCATCGACTGATCTATACAAAGACCAGCTATTTACATGCAGAAACGTTAGAAAAGAACAATAAA
ATGATGATTAATTTAGATGGTGAATATGGAGGCGATGCTCCAATGACCTTTAAAAATATGCATCAACACATTGAAATTTTT
GCAAATGGTGATGCACTGCCGTCCAATGCAATTATGGGTTCTGTCTTAACTGGTAGCGATGAGATTGTCGTAGAATCAGAA
GACGAAGAGGAAGAAGCATATAACGAAGCCAGCAAAGAATTTGTCAAAGAAGTTGAACGACTAACAGACGAAGATATTGAT
GGCGATGGAAAGATTGCGGAAAAAGAAAAGCAC
EF0775
Seq ID 384
ATGACCAATACGGTGAAAGTGAAAGACGACAGTCTGGCTGATTGTAAACGGATATTGGAAGGACAAGCTACTTTCCCAGTT
CAAGCGGGTGAAACGGAACCAGTCGATTTAGTAGTTGTTGAAGATGCTAGTGGTAGTTTTTCAGATAATTTTCCACATGTA
AGACAAGCGATTGATGAAGTGGTTCAAGGCTTATCTGATCAAGACCGCGTGATGCTGGCTTCATATCGCGGCGGAAAACAA
TTTATGTTTCCTGATGGAAAGACAAAAATTAATTCAGCTGATTATGATATGAATGTGCGCGTCAATACGCAATTGACTTAT
GATAAAAGCCAATTTGTCTCTGGTTTTGGAGACGTTCGGACGTATGGTGGTACGCCAACCGCCCCAGGATTGAAACTCGCT
TTAGATACGTACAATCAAACACACGGAGATTTAACGAATCGAAAAACGTATTTCCTATTAGTGACAGATGGGGTCGCTAAT
ACACGTTTAGATGGTTACTTGCATAAGACCAATACCAATGATTCAATCAATGAATATCCAGATCCAAGACATCCTCTTCAA
GTCTCAGTGGAATATAGTAATGACTACCAAGGTGCAGCAGCAGAAGTTTTAGCGTTAAACCAAGAAATTACTAACCAAGGC
TATGAAATGATTAATGCGTATTGGGAAAGTGTTGAATCTTTAAGTTCAGTGAATTCATACTTTGATAAATATAAAACAGAA
GTGGGTCCTTTTGTAAAACAAGAGTTGCAACAAGGGTCTAGCACACCAGAAGATTTTATTACAAGCCAATCTATTGATGAT
TTTACAACCCAATTAAAACAAATTGTCAAAGATCGTCTGGCGCAATCGACACCAGCAACAGCTTCATTAACGATTGCCAAT
CAATTTGATATTCAATCTGCGACCGCTACGGACGATGCTGGAAATGATGTGCCTGTTCAAATTAACGGACAAACCATTTCA
GCAACTAGTACAGAAGGTTACGTAGGAAACATCACGATTCACTACGAAGTCAAAGAAAATACAGCGATTGATGCAGCAACC
CTTGTAAGTAGTGGGACAATGAATCAAGGAACAATTGCTAAGGAATTTCCAGAAGCGACGATTCCTAAAAATGACAATGCG
CATGCGTGTGACGTGACGCCAGAAGATCCAACGATTACAAAAGATATCGAAAATCAAGAACACTTAGATTTAACCAATCGT
GAAGATAGTTTCGATTGGCATGTCAAAACAGCCTTTGGCAACGAAACCAGTACTTGGACCCAAGCCAGCATGGTGGATGAC
ATTAATAAAGTGCTAGATATCATTGATGTGAAAGTCACCGACGAAAATGGTAAAGATGTTACAGCTAACGGCACAGTAACA
CAAGAAAATAACAAAGTAACTTTTGAAATGAACAAACAAGCAGACAGCTATGACTATTTAAGTGGTCATACGTATACAATG
ACTATCACCACTAAAATTAAAACTGACGCAACGGACGAAGAATTAGCGCCTTACATTGAACAAGGCGGGATTCCCAACCAA
GCCGACTTAAACTTTGGCAATGAAGGTGACGTGTTACATTCCAACAAACCAACCGTAACACCACCGCCAGTTGATCCAAAT
ATTGCTAAAGACGTAGAAGGACAAGAACATTTAGATTTAACCAACCGCGATCAAGAATTTAAATGGAACGTCAAAACAGCT
TTCGGTAACGAAACAAGCACTTGGACCCAAGCCAGCATGGTAGATGACATTAATAAAGTGTTAGACATCACTGATGTAAAA
GTCACAGATGAAAATGGTAAAGATGTTACAGCTAACGGCAAAGTAACACAAGAAAATAACAAAGTAACTTTTGAAATGAAC
AAACAAGCAGACAGCTATGACTATTTAAGTGGTCATACGTACACAATGACCATTACTACTAAAATCAAAGCTAGCGCAACG
GACGAAGAATTAGCACCTTATATTGAACAAGGTGGCATTCCCAACCAAGCCGACTTGAACTTTGGCAACGAAGGTGACGTG
TTGCATTCCAACAAACCAACCGTAACACCACCTGCACCAACGCCAGAAGATCCAACGATTACAAAAGATATCGAAGGCCAA
GAACATTTAGATTTAACCAACCGTGACCAAGAATTTAAATGGAACGTCAAAACAGCTTTCGGTAACGAAACAAGCACATGG
ACCCAAGCCAGCATGGTGGATGACATTAATAAAGTGTTAGACATCACAGACGTGAAAGTTACTGATGAAAATGGCAAAGAT
GTTACAGATAATGGCATAGTAACACAAGAAAATAACAAAGTAACTTTTACTATGAACAAAAAAGATGACAGCTACTCTTAC
TTAGCTGGTCATACATACACAATGACTATTACCACTAAAATTAAAACTGACGCAACGGATGAAGAATTAGCGCCTTATATT
GAACAAGGCGGGATTCCCAACCAAGCCGACTTAAACTTTGGCAACGAAGGTGACGTGTTGCATTCCAACAAGCCAACCGTA
ACACCGCCTGCACCAACGCCAGAAGACCCAAAAAAACCTGAACCTAAACAACCGCTAAAACCGAAAAAACCGTTGACGCCT
ACAAATCATCAAGCACCAACGAACCCAGTCAATTTTGGAAAATCAGCAAGTAAAGGAATTCATTTACCAATGACTAATACA
ACAGTAAATCCACTTTACATGATCGCAGGTTTAATTGTCCTTATAGTGGCTATTAGCTTTGGCATAACAAAAAATAAAAAA
AGAAAAAAT
EF0779
Seq ID 385
ATGTCTGCTTGGCAAACATTTAAAAATGGAACAAGGCAATTTTTTAAAGACATCTTGCAGTATTTATGGTTATTTTTTACT
TTAAATGTCTTATTATTATTGGTGGGTGGGGCTTTTAGTTGGGCTACTTCCAACGCACTAAAAACTCAAGGTATTCCTTAT
CTTTCGTTTAATAACCTGAATTTATTGTTAGAAAAGCCGCTTGCTCTGGTGTTATTAATTCTCTTGTTGTTACTTTTTTTA
GGTGCAGTTTTTTATCAGTTTACCTTTTTATTATTAGGGATTTTTCAAATACGGCAAGACCATCGCTTTCATTTTAAAGGA
GTTACCAAGGCATCTTTTAAGGTTCTTAAAAAGCAAGGTGCCCGTTCGTGGTTATTCTTTTTCGGTTATTTTGTTGTCATT
GTCCCTTTTGGTAACCTGATTTTCCAAAGTAATCTATTGACGAAATTTGTGATTCCAGATTTTATTGTTGAATTTTTGTCC
CAACGAATTCCTTACTTGGTGGGGTTACTGGCCTTTGGGTTATTAGTCTGGTATTTAGCTATTCGTTTCATTTACACGCTT
CCATTAATGATTCTGGAGAGAAAAAAAGCGGGCGAGGCAGTTAAGGCTAGTTGGTCAATGACAAACAAACGATTATGGTTC
ATTATTCGTAATATTGCATTTGTGACGATCGCGGTATTTGTTTCAACGTATGTTATTTATGTGTTACTTTATCTTCTACAG
TTGAAGTTAGATACCTTGAGCGATACCATTAGCTTGCTAGGTGGGATTTTAAATTTAACCGTTGTCCAATTTTTACAGTTT
GTCTCTAATGCTTGGCTTTCAGTATTACTGATTAATTTCTTATATACACAATTAAATGTTCAAGCAGAAACGACGACAAAA
GTTGCCTTTGACAAAGAAACGAAACGTAATAAATTAGTGACTATCGGGATGGGCCTAGGACTATTCACCATTTTTGGCGGC
TATATAATTTTCAACGCTGTTTACTTAACAGGGCTATTAGAATCAAAACCTTTGATTATTTCGCATCGAGGGGTCACCAAT
AGTAATGGCGTACAAAATACCATTCCTGCTATGGAAAGAACCATTAAATTTAAACCAGATTATATTGAAATTGATGTTCAA
GAAACCAAAGACCATCAATTTGTTGTGATGCATGATGCAAACCTTCAAGAACTCGCCGGCGTTGATGGCACGCCGCAAGAA
TTTACGTTGGCTGAATTAACAAAAATGACCGTTAAAGAAAATGGCCAAGAAGCACCAATTGCCAGTTTTGATGATTATTTA
GCGAAAGCAAACCAAGCCAAACAAAAATTACTAGTAGAAATTAAAACGTCTAAACAAGATAGTCAAGGTGCGCTTTCTAAC
TTTATTGAAAAATATGAACGTCCTCTGATAAAGAATAACCATCAGGTTCAATCTCTCGACTACAATGTGATCAAAGCGTTT
AAAAAAGCCAAATCAAAAGTAAAAGTTAGTTTTATTTTGCCCTATAATTTCACTTTTCCAGAAACACAAGCTGATTTGTAT
ACAATGGAAGCTACTACGTTGAATGATACCTTCATTTTAAAAGCTGACCAACAGAAAAAAGCAGTTTATGCTTGGACAGTT
AATGACTCAGAGGTATTGAGTAAAATGCTCTTTATGGACGTTGCTGGAGTGATTACCGATGATTTGGAGTTAGTCAATGAA
GAAGTCAATGACTTTGAAAAAAATCCGTCGTATGCGGATCGTATTTTGCATTATATTTTTATGCTACCAAGTGTGGCTTCT
CAA
EF1091
Seq ID 386
ATGATAACAGATGAGAATGATAAAACGAATATTAATATCGAGTTAAATCTTCTCAACCAAACAGAGCAGCCATTACAACGA
GAAATTCAATTGAAAAATGCACAGTTCATGGATACTGCTGTAATTGAAAAAGACGGATATTCTTACCAAGTGACTAATGGT
ACGCTTTATCTGACTTTGGACGCACAAGTAAAAAAGCCGGTACAGCTTTCGTTAGCTGTTGAGCAAAGTTCGCTTCAAACA
GCTCAGCCACCTAAGTTATTGTATGAAAACAACGAATATGATGTTTCAGTTACTTCTGAAAAAATAACAGTAGAGGATTCT
GCTAAAGAATCAACTGAACCAGAAAAAATAACTGTACCAGAAAATACGAAAGAAACTAACAAAAATGATTCGGCTCCAGAA
AAAACAGAACAGCCGACCGCAACAGAAGAGGTAACCAATCCATTTGCAGAAGCAAGAATGGCGCCAGCTACTTTGAGAGCG
AATCTGGCACTGCCTTTAATTGCACCACAATACACGACGGATAATTCTGGGACTTATCCGACAGCTAATTGGCAGCCCACA
GGCAATCAAAATGTGTTAAACCATCAAGGGAATAAAGACGGTAGTGCACAATGGGACGGCCAAACGAGTTGGAATGGGGAC
CCTACTAATCGCACAAATTCTTATATTGAGTATGGCGGTACAGGAGACCAAGCCGATTATGCCATCCGAAAATATGCTAGA
GAAACAACAACACCAGGGCTTTTTGATGTATATCTTAATGTGCGTGGGAATGTTCAGAAAGAAATCACGCCATTGGATTTG
GTCTTAGTCGTTGACTGGTCCGGTAGTATGAATGAAAACAATCGGATTGGTGAAGTTCAAAAAGGAGTGAACCGTTTTGTT
GATACATTGGCAGATAGCGGTATTACCAATAACATCAACATGGGCTATGTTGGCTACTCAAGTGACGGTTATAATAACAAC
GCCATTCAAATGGGGCCGTTTGATACAGTCAAAAATCCAATTAAAAATATTACGCCAAGTAGCACTAGAGGAGGAACTTTC
ACTCAAAAAGCATTAAGAGATGCTGGTGATATGTTAGCAACGCCAAATGGACATAAGAAAGTCATTGTACTTTTAACGGAT
GGCGTCCCAACCTTCTCTTATAAAGTGAGTCGAGTTCAAACAGAGGCGGATGGTCGCTTTTACGGGACACAATTTACGAAT
CGACAAGATCAACCAGGTAGCACTTCTTATATCTCTGGTAGCTATAATGCGCCAGATCAAAACAATATCAATAAACGGATT
AACAGTACGTTTATCGCCACGATAGGTGAGGCAATGGTCTTAAAACAACGTGGGATTGAAATACATGGATTGGGCATTCAA
TTGCAAAGCGATCCACGAGCTAATTTATCTAAACAACAAGTTGAAGATAAAATGCGTGAGATGGTGTCAGCCGATGAAAAT
GGAGACCTTTATTATGAATCCGCGGATTATGCACCAGACATTTCTGATTATTTAGCGAAAAAAGCCGTTCAGATTTCAGGA
ACGGTTGTAAACGGAAAAGTAGTTGATCCAATTGCTGAACCTTTTAAATACGAGCCAAATACATTATCAATGAAAAGTGTG
GGTCCTGTTCAGGTTCAAACATTACCAGAAGTGTCGCTAACAGGCGCTACAATTAATAGTAATGAGATTTATTTGGGTAAA
GGGCAAGAAATTCAAATTCATTATCAAGTACGTATTCAAACAGAGTCAGAAAACTTCAAACCTGATTTTTGGTATCAAATG
AATGGTCGGACAACGTTTCAGCCATTAGCCACGGCCCCTGAAAAAGTTGATTTTGGGGTTCCTTCGGGAAAAGCACCTGGC
GTGAAGTTAAACGTGAAAAAAATCTGGGAAGAGTATGATCAAGACCCGACAAGTCGGCCAGATAATGTGATTTATGAAATT
AGTAGAAAGCAAGTAACTGACACAGCCAACTGGCAAACTGGGTATATTAAATTATCAAAACCAGAAAATGATACCAGCAAT
AGTTGGGAGCGCAAAAATGTAACCCAACTTTCCAAAACCGCGGATGAAAGCTATCAAGAAGTTCTTGGGCTTCCCCAATAC
AACAATCAAGGACAAGCTTTCAATTATCAAACAACCCGTGAATTAGCAGTTCCTGGTTACAGTCAAGAAAAAATCGACGAT
ACTACTTGGAAAAACACGAAGCAGTTCAAGCCATTAGATTTAAAAGTAATCAAAAATTCTTCCTCAGGTGAGAAAAACTTA
GTGGGAGCCGTCTTTGAATTGAGTGGTAAAAATGTTCAAACAACATTAGTGGACAATAAAGATGGTAGCTATTCCTTGCCA
AAAGATGTGCGCCTACAAAAAGGGGAACGCTATACATTAACTGAAGTAAAAGCACCTGCAGGACATGAGTTAGGCAAGAAA
ACGACTTGGCAAATTGAGGTGAGTGAGCAAGGCAAAGTAAGCATCGATGGACAAGAAGTGACCACCACAAATCAAGTTATT
CCATTGGAAATTGAAAATAAATTTTCTTCTTTGCCAATCAGAATTAGAAAATACACCATGCAAAATGGCAAACAAGTGAAC
TTAGCAGAGGCGACTTTTGCGTTGCAAAGAAAAAATGCTCAAGGAAGTTACCAAACTGTGGCAACTCAAAAAACAGATACT
ACAGGATTGAGCTATTTTAAAATTAGTGAACCTGGTGAGTATCGAATGGTGGAACAATCAGGACCATTAGGCTACGACACT
CTTGCTGGAAATTATGAATTTACTGTTGATAAATATGGGAAAATTCACTATGCAGGCAAAAATATTGAAGAAAATGCGCCA
GAATGGACACTGACACATCAAAATAATTTGAAACCTTTTGACTTAACAGTTAATAAAAAAGCCGATAATCAGACGCCACTT
AAAGGAGCGAAATTCCGTTTAACAGGACCAGATACGGATATTGAATTACCAAAAGATGGCAAAGAAACGGATACTTTTGTT
TTTGAAAACTTAAAACCAGGGAAATATGTTCTAACAGAAACCTTTACGCCAGAAGGATATCAGGGGTTAAAAGAACCAATC
GAATTAATAATTCGTGAAGATGGTTCAGTCACGATAGATGGGGAAAAAGTAGCAGATGTTTTAATTTCTGGAGAGAAGAAT
AATCAAATTACTTTAGACGTTACGAACCAAGCAAAGGTTCCTTTACCTGAAACTGGTGGCATAGGACGCTTGTGGTTTTAC
TTGATAGCGATTAGTACATTCGTGATAGCGGGTGTTTATCTCTTTATTAGACGACCAGAAGGGAGTGTG
EF1323
Seq ID 387
ATGGGGATTTTGTTACCGATTATTATTGCAGTTTTAGTATTATTAATGTTATTAATTGTGTTTGTTTCTAAATATCAGACA
GCCAAACCTGATGAAGCGTTAATTATCAGCGGGAGCTATCTAGGCTCTAAAAATGTTCATGTAGACGAAGGTGGCAACAAA
ATTAAAATCGTTCGTGGCGGTGGTGCGTTTGTCTTACCAGTGTTCCAACGTTCAAATCGAATTAGTTTGCTTTCAAGTAAA
TTAGATGTTTCCACACCAGAAGTGTACACAGAACAAGGGGTTCCTGTGATGTGTGATGGGACATCAATCATTAAAATTGGT
TCGTCAGTTGAAGAAATTGCGACAGCAGCGGAACAATTTTTAGGAAAAACAACGGAAGAATTAGAAAATGAAGCACGTGAA
GTATTAGAAGGACATTTACGTTCGATTTTAGGTTCAATGACAGTGGAAGAAATTTACCAAAATCGTGATTAATTTAGCCAA
AGTGTACAAGAAGTTGCCAGTGTTGACTTAGCTAAAATGGGCTTAGTTATTGTGTCGTTCACAATTAAAGAAGTTCGTGAT
AAAAATGGATACTTGGATTCATTAGGGAAACCAAGAATCGCTCAAGTTAAACGTGATGCAGATATTGCAGAAGCAGAAGCC
TTGAAAGAAACTCGCATCAAAAAAGCAGAAGCAGAAAAAGAATCACAACAAGCGGAATTGCAACGTCAAACAGAAATTGCA
GAAGCTTCAAAAGAAAAAGAATTGAAATTAGCGTTATACAAACAAGAACAAGATATTGCCAAAGCCAAAGCAGACCAAGCC
TATAACTTGGAAAGCGCGCGTGCACAACAACACGTGGTGGAACAAGAAATGGAAGTCAAAGTCGTTGAACGTCAAAAACAA
ATTGAGTTAGAAGAAAAAGAAATTACGCGTCGTGAAAAACAATACGACTCAGAAGTGAAGAAAAAAGCCGATGCAGATCGT
TACGCACGCGAACAAGAAGCTCTTGCTCAAAAGGCACGCGAAGTGGCAGAAGCCGAAGCAGAACGCTTCAAAGTTGAAGCA
TTAGCCGAAGCGGAAGCCAACAAAACGCGTTTAACAGGTCAAGCGCAAGCCGAAGCTATTTTAGCTCGTGGTGCAGCGGAA
GCCGAAGCCAAACAAAAGATTGCTGATGCCTTCAAAGAATATGGTGAAGCAGCTGTGTTAAGTATGGTGATGGAAATGTTA
CCACAATTAATGAAAGAAGCAGCACAACCATTGGGCAACATCGACAAGATTTCAGTGGTAGATACAGGCGCAGGTGGCGAA
AATTCTGGCGCCAACCGTATTACAAATTATGCAACAAATTTGTTGGCTGGTACACAAGAAACACTAAAAGAAACAACGGGC
CTCGACGTGAAAGAGCTAATTGAAAACTTTTCTAAAAAAGGTACCTCAAATAGCGTGAACTATCATGCAACAGAAGGTTCA
GAAAAAGAA
EF1355
Seq ID 388
ATGGCTTATCAGTTTAAATTACCGGATATCGGTGAAGGGATTGCCGAAGGCGAAATCGTTAAATGGTTTGTAAAACCTGGC
GATACAATCAACGAAGACGATACGTTATTAGAAGTACAAAATGACAAATCAGTGGAAGAAATTCCATCACCAGTAACAGGT
ACTGTAAAAAATATCGTTGTACCAGAAGGAACAGTTGCAAACGTTGGTGACGTGTTAATCGAAATCGACGCACCTGGTCAC
GAAGATAACGATGCAGCACCAGCAGCTCCTGCACAAGAACAAACACCAGCACAACCTGCTGCTGTACCAACAACCGAAGCA
GCTGGCGGATTTTTCCAATTCAAATTACCAGACATCGGTGAAGGAATTGCCGAAGGCGAAATCGTTAAATGGTTCGTTAAA
GCGGGCGACACAATTAATGAAGATGATTCATTATTAGAAGTACAAAATGACAAATCAGTAGAAGAAATTCCATCACCAGTA
ACAGGTACTGTAAAAAATATCGTTGTACCAGAAGGAACAGTTGCCAATGTGGGTGACGTGTTAGTTGAAATTGACGCACCT
GGTCATAATTCAGCAGCACCGTCAGTCGCAGCACCAGCTACTGACGCTCCTAAAGCGGAAGCATCAGCTCCAGCCGCTTCA
ACAGGCGTAGTTGCAGCCGCTGATCCAAACAAACGCGTTTTAGCAATGCCATCTGTTCGTCAGTATGCGCGTGAAAAAGAC
GTTGATATTACACAAGTAACTGCAACTGGTAAAGGTGGCCGTGTCATTAAAGCGGATATTGATGCCTTTGTCTCTGGTGGT
TCTCAAGCAGCCCCAGCTACTGAAGCTGCCGCAACAGAAGCAGCACCTAAAGCGGAAGCAGCTGCACCTAAAGCAGCGCCA
AAAGCCTTTACTTCTGATTTAGGCGAAATGGAAACACGTGAAAAAATGACACCAACACGTAAAGCAATTGCTAAAGCAATG
GTTAACAGCAAACACACTGCTCCTCACGTAACATTACATGATGAAGTAGAAGTTTCTAAATTATGGGATCACCGTAAGAAA
TTTAAAGATGTTGCTGCTGCAAATGGTACAAAATTAACATTCTTACCATACGTTGTAAAAGCATTGACTTCAACTGTTCAA
AAATTCCCAATCTTGAATGCATCAATCGATGACGCAGCACAAGAAATTGTTTACAAAAATTACTTTAACATTGGTATCGCT
ACTGATACAGATCATGGCTTATATGTACCAAATGTTAAAAATGCTAATACGAAGAGCATGTTTGCTATCGCTGATGAAATC
AACGAAAAAGCAGCATTGGCTATCGAAGGTAAATTAACTGCACAAGATATGCGTGATGGTACAATCACAATTAGTAACATT
GGTTCAGTCGGTGGCGGCTGGTTTACACCAGTAATCAACTACCCTGAAGTTGCTATTTTAGGCGTTGGTACAATTGCACAA
GAACCAGTTGTTAATGCAGACGGCGAAATCGTTGTGGGACGCATGATGAAATTATCATTAAGCTTTGACCACCGTATCGTT
GACGGCGCAACTGCTCAAAAAGCAATGAACAACATTAAACGCTTATTAGCTGATCCAGAATTACTATTAATGGAAGGA
EF1699
Seq ID 389
ATGGAATATACAATTAAAAAAATGGCTTCACTATCTGGCGTCAGTGCACGGACGTTACGATATTATGATGAAATTGGTCTT
CTTCAACCAGCCAGAATTAATTCTTCTGGTTATCGAATTTACGGGCAAGCCGAGGTGAATCGTTTGCAACAAATTCTTTTT
TACCGTGAATTGGATCTGAAACTAGATGAAATTAAAGAAATTTTGGAGCAACCTGACTTTAATGTTGAACAGGCTTTATAC
GAGCATCAACAAAAACTATTGGAAAAGCGCAATGAAATTGATCGTCTTTTAGCCTCTGTTCAACAAACCTTACACCATTAC
AAAGGAGAGATAAACATGTCAGATCAGCAAAAATTCGAAGCATTCAAACAACAAAAAGTTCAAGAAAACGAAGAAAAATAT
GGTAAAGAGATTCGGGAAAAATATGGCAACGAAACGATTGAACAAGCCAACAAAAAATATTTAAACTTGACGGAAAAAGAC
ATGCAAGCAATGCAGAACGTGGAAAAAGATTTATTTTCTAAGTTAGCCATGTATCAAAAATCACCCAAGTTGACTAGCCAA
CTTGCGCAAGAAATTTTCCAATTACACAAAGATTGGTTAATGTACTCTTGGTCAAGCTATTCGCCAGAAGCACACAAAGGG
TTAGGCCTTATGTATGTTGGCGATGAACGATTTACGTCATATTATGAACAACACGGCGCTGGTTTTGCCGAAGCGTTAAAC
GCAATTATCCAAAACTACGCT
EF1744
Seq ID 390
ATGGCTAAAAAAGGCGGATTTTTCTTAGGAGCAGTAATTGGTGGAACAGCAGCAGCCGTTGCCGCATTATTACTTGCACCA
AAATCAGGTAAAGAATTACGTGATGATTTATCAAATCAAACAGATGATTTAAAAAACAAAGCGCAAGATTACACAGATTAT
GCTGTTCAAAAAGGAACAGAATTAACAGAAATCGCAAAACAAAAAGCCGGCGTTTTATCAGATCAAGCCTCTGATTTGGCA
GGTTCTGTCAAAGAAAAAACAAAAGATTCATTGGATAAAGCACAAGGTGTTTCTGGCGACATGCTTGATAACTTTAAAAAA
CAAACAGGTGATTTATCTGATCAATTTAAAAAAGCAGCTGACGATGCTCAAGATCACGCAGAAGATTTAGGTGAAATTGCC
GAAGATGCAGCAGAAGATATCTATATTGACGTTAAAGATTCTGCGGCAGCGGCCAAAGAAACTGTTTCTGCTGGTGTCGAT
GAAGCAAAAGAAACCACCAAAGATGTTCCTGAAAAAGCTGCAGAAGCAAAAGAAGATGTTAAAGATGCAGCGAAAGACGTA
AAAAAAGAATTTAAAGGG
EF1752
Seq ID 391
ATGAAAAGAAAACTAACAAAATCTCCGAATAACGTGGTTTTAACAGGAAGCTTAGCTGGGATTGCTGATTGGCTAGGAATT
GACCCAACAATTATTCGTGTCGTTTATGTCTTGCTCAGCTTTTTCTCAGCAGGATTTCCAGGCATTCTCTTATACATTGCT
TTAGCAGTCTTGATCCCTTCTGGAAGAACAGGAAGCGATCGCGGCTACGGCCATCAAAATCCTTATAATAGAAATGTTCGC
AATGAAAATCCTTATGCTGCCAATAAAAAGCAACGTAAAGAAGCGGAAAAAATTGATGATGACGAATGGAGTGACTTT
EF1753
Seq ID 392
ATGAAAGAAAGAGAACGCGTATTAGAATTAGTGAAAAAAGGTATTCTAACGTCAGAAGAAGCGTTAATTTTATTAGAAAAT
ATGGCAACTGAAAAAGATGAAAAACAAATCGAAAAAGCTGCTGAAAAAGTTGATACACAAAATATTGGAACAACAAATAAA
GAAGATCAAGTCGCAGATTTAATGAATGCATTAGAAAAAGGCGAATCAGAAGGACCTACTGTTGATTCGTTTGAAGAAAAT
ACACAGGACAGTGCAGAAAAAGATCGTGAAAACTTAGAAAGAATTCTTGATGAGTTAGCAACAAAAGCCAATCGTGCTTCG
GCTGAATTGGACGAAGTCAACGCAGAAATTGCCGGCATCAAAGAAGAAATTAAAGAAGTCGCAGAAGAAATTGGAACATTA
GATACAAAAGAAGAATTAGATGCATTAACAGAAGATGAACAAGTTCAACGAAAAGACTTGCACGTTTTACTTGCACAATTA
GAAGAAAAATTAGCGACTCAAAGTACTGAAAAAACAGCACTGGAAGAAGAACTAAAAAACATTCGCAAAGAACAATGGAAA
GGTCAATGGAATGATACAAAAGAAAAAGTTTCTTCTCAATTCTCTGAAGAGTGGAAAGATCAAGCCACAGACACCTTTAAC
CAAGTCGGCGGCAAAGTTGCCGAAGTTGGTGGTCAAGTGGGAGAATTCTTGAAAAAAACATTTAATTCTTTCAGTGATACC
ATGAATGATAATGTGGAATGGAAAGACATTAAAATGAAAGTTCCTGGTGTGGCAACAACTAAGTTTGAACATGAGTTTAAC
TATCCAAATCCACAAGCAAGTTTAATTGATGTCAAGGTAGCAAATGGTACCGTGGTTTTCAAAACTTGGGATCAAGAAGAT
GTGAAAGTCGAAGCAAAAATCAAATTATATGGTAAAATGGCAGGAGATTCACCAATGGAAGCTTTCTTAGAACGAAGTGAC
ATTGATGTGGATGATGAAACGATTTCTTTCCAAGTGCCAAACAAACGGGTGAAAGCAGATTTAACGTTCTATTTACCAAAA
CGCACTTACGATCATGTATCTGTTAAATTATTAAACGGAAATGTCTTAGTAGAAGAGTTAACAGCGAAAGATGTTTACACA
AAATCAACGAATGGGACGATTACGTTTAAAAAAATTGATGCAACTATGTTAGAAATTGAAGGTGTGAATGGTGAAATTAAA
GTCCTAGAAGGAACGATTTTAGATAACATTATTGAAACAGTCAATGGTGATGTGTCTATTTCCGCGGCACCAGAAAGTCTA
AGTGTCTCTCTGATTAATGGAGATATCCGCATTACAGCTAAAGAAAAAACGCTTCGCCGTGTTGAAGCAAGCTCTGCAAAT
GGGAATATCAAATTGGCCTTGCCAAATGACTTAGGTGTTGAAGGGCAAGTGAAAACTAATTTAGGTAGTATTAACAGGCGT
TTAACAGATATTGAAGTTGTTCGTGAAAAGAAAGATCGCGGCAATCAACAATTACATTTTAGACGTGTACTGGAAGAATCA
ATGGCTCAAATTAATGCTTCTACAACAACGGGAAGTATTTTCCTAAAAGATACGGATAAA
EF1791
Seq ID 393
ATGAAAAAGTATTTAAAAATCACAATGGTTTGTATTTTATTGGTAGGATTTTTAGCTGGGTGTACCAATAAAAATGAAAAT
AAAAAGAAACAGAAAAATACCAAAGAAGCCGTTCAACTGATGTCACCCTCGGAATTAACAACGCTCAACACCTCTGTATTA
TTGGATTTTCCAGATGCTATTGTCCAAACTGCAGCGTTTGAAGGGTTATATAGTTTAGATGAACAAGACCAATTGGTACCA
GCCGTAGCAAAAGCATTGCCGATGATTTCAGAAGATGGAAAAACCTACACGATTTCTTTGAGAAAAGAAGCGGTTTGGAGT
AACGATGATCCTGTCACAGCACATGATTTTGAATATGCTTGGAAAAAAATGATTGATCCTAAAAACGGCTTTGTTTATAGC
TTCCTCATCGTTGAAACAATTCAAAATGGTGCAGAAATCTCAGCGGGGAAATTAGCACCCAATGAACTAGGTGTCACAGCT
GTGGATGATTATACATTAAAGGTGACGCTCAAAGAGCCAAAACCGTACTTTACGTCCTTGTTAGCTTTTCCGACATTTTTC
CCGCAAAATCAAAAAGTAGTCGAACAATTTGGTGCGGACTATGGAACTGCTAGTGATAAAGTCGTCTATAATGGTCCGTTC
GTGGTAAAAGATTGGCAGCAAACAAAGATGGACTGGCAACTAGCAAAAAATAATCGCTATTGGGATCACCAGAACGTGCGC
TCAGACATTATCAATTATACAGTTATCAAAGAAACATCTACCGCATTGAATCTTTTTGAAGATGGACAATTAGATGTGCCT
ACACTAAGTGGTGAACTGGCGCAACAGAATAAAAATAATACGTTGTATCATTCGTATCCAACAGCGACAATGAACTATTTG
CGCTAAAATCAAAAACGGAAAGGGCAAGCAACGCCGCTTGCAAACGAAAACCTGCGTAAAGCATTGGCTTTAGGAATAGAT
AAAGAAAATCTAGTCAATAATATTATTGCAGATGGTTCTAAAGCGCTACATGGTGCGATTACGGAAGGCTTTGTGGCGAAT
CCCACAACGGGTCTCGATTTTCGTCAAGAAGCAGGTAATTTAATGGTTTATAACAAAGAAAAAGCGCAAAGTTATTGGAAA
AAAGCACAAGCAGAATTAGGAGAAAAGGTTAACGTTGAATTGATGGTAACAGATGATGGTTCTTACAAAAAAATTGGTGAA
AGTTTGCAAGGCTCGCTACAAGAATTGTTTCCTGGTTTGACAATAGAGCTAACCGCATTGCCGACTGAAGCTGCATTGAAC
TTTGGGCGAGAAAGTGACTATGATTTATTCTTAATTTACTGGACACCAGACTATCAAGACCCTATTTCTACCCTGATGACT
TTATACAAGGGCAATGATCGCAATTATCAGAACCCTGTCTATGACAAATTATTAGATGAAGCAGCCACAACCTATGCCTTA
GAGCCAGAAAAAAGATGGGCGACACTGATTGCAGCTGAAAAAGAAGTGATTGAAACGACTGCTGGCATGATTCCACTTAGC
CAAAATGAACAAACAGTCCTGCAAAATGATAAAGTCAAAGGCTTGAATTTTCATACCTTTGGCGCTCCATTAACGTTAAAA
AATGTTTATAAGGAAAAA
EF1800
Seq ID 394
ATGAAACATGGAAAAATAAAACGATTTAGTACATTGACACTATTGGCAAGCGCAACGATTTTAGTACCATTAAGTACGTCT
GCAGAAGAAACAACAAACAGCAGCACTGAAACAAGCTCATCAATGGTGGAACCTACCGCAACAGAAGAAAAATTGTGGCAA
TCTGATTTTCCAGGAGGGAAAACTGGTGAGTGGCAAGATGTGATTGGTAAAACAAATCGAGAATTAGCAGGAGAGTCATTG
GCGATTTCACGAGATGCAGCAGCAGGTAATAATGCCGTATCTTTAAATTTAGATTCCCCTAAATTAGCTGATGGTGAAGTA
GAAACAAAGTTTAAATACACTGCTGGAAGCGGTCGAACGGGTGTGATTATACGTGGTAATACCAAAGATAGCTGGGTTTTT
GTAGGCTATAACGCGAATGGCAAATGGTTAGTTGAAAGCCCTAATTCGTGGAATGATTCAATTTCTGGGCCAACGTTAAAT
GAAGATACGAATTATTTGTTGAAAGTACGTTATGTTGGTGAAAAAATTACTATCTGGCTTAACACCACGTTGATTTACGAA
GGAGAACCTGTTTTGGCCAATGGAGACAAAATTCCAACAGAAGCCGGACATGTGGGTGTCCGTTTGTGGTACGACAAAAAA
ATTGTCAATTATGACTATTTTAAAAATGGCCCCGTAGATAGCATTCCAGAAATTGTGCCAGAAGTGACACAAATTGCGCCA
GTCAAAGTTTTTACAAAAATTGGTGTCGCACCAAAATTACCGAAACAAGTAAAAGTGACCTATAATACTGGTAAAGAAGCC
AATGAAGCAGTCCGTTGGAATGAAATCGATCCTGATGCATATAAAGAACCAGGAACTTTTGAAGTCGACGGTACTTTGGAA
AATACAAACATCAAAGCAAAAGCCAGCATTGTTGTTGCTAAAGACAATGAAGCTGAAAAAGGCGACAAAATCTCTTCCGCA
GATTTAACAGCCGTGGTTGATCCACAATTTCCACGAATTATTCGCTACGAAGACCCTCAGAGTAATCAAGTGATTTTTAAT
GGCCAACACGAGAAAATTGACCAAGTAATGATTGATGGCAAAGCATATAAAGCAACTGCTGAAAAACAGAAGAGTGAAGCA
AATCAAGCCGTTTATAACGTAGCTGTCCCAGAAATTGGTTTGCGTTTCACAACGACATTGACTGTTTCCGAAGGCCAAGAA
TTAGCTATGAAACTCTCAGATATTCGTGAAGAAGGAACCAAAATACACACAATTTCAATTCCAAATCAAGGCTTGATTTCT
GTCAATAGTACAGATGAAGGGGCGACTTTTGCTGGCGTTGTGATGAATACTGGGACAAATGCAAATAACGGAAATAAAAAT
GGTGATACTATCCAAGATTTAACTACAACAAGCCAAGAAGAAACGAAAAAATATATGTATGGTTTCTTAAATACGGCGAAT
TATGCTGCAAGTTTTTGGACGAACGCCTATGGAGACGGCTCTGTCGATGGTAGTGACAACAATCGAATCCATAAACAAACA
AAAGAAGCGGCGACTGGTTTTGTAACAACCTTGTCAAGTGGGGCATGGACCTATCGACCATTTGATGCACCGGAAGATTAC
ACAACTGGAGAAACGCCAGAAGTGAAAGTTAAATTCTCAAAAGATAGCAACGACGACAATCGGGTGGATTGGCAAGATGCG
GCAATTGGGTTCCGTTCAATTATGAATAACCCAATGGGTGCGGAAAAACTCCCTGAATTAGTCAACCAACGGATTCCTTTT
AACTTTGCTAGTCAGGCGACAAACCCATTCTTAGTGACGTTAGACGAATCAAAACGTATTTACAATTTAACAGATGGATTA
GGACAAATGAATTTACTAAAAGGGTATCAAAATGAAGGACATGATTCTGCGCATCCAGATTACGGTGCTATTGGTCAGCGA
CCTGGTGGGGAACAAGCGTTGAATCAATTAATTGATGAAGGACATAAATTAAATGCCGTTTTCGGTGTGCATATTAATGAC
ACCGAGTCTTACCCAGAAGCAAAAGGATTTAATGAGGAATTAGTTGATCCAACGAAGCGTGGCTGGGATTGGTTAGATCCG
TCTTATTTTATTAAACAAAGACCCGATACATTGAGTGGTCGTCGCTATGAGCGCTTTAAAGAATTAAAACAAAAAGCACCG
AATCTAGATTATATTTATGTTGATGTTTGGGGCAACCAAGGCGAATCAGGTTGGGCAAGTCGTCAACTAAGTAAAGAAATT
AATTCACTCGGTTGGTTTACAACCAATGAATTTCCGAATGCTTTAGAGTATGACTCGGTTTGGAACCATTGGTCTGCAGAA
AAAGATTACGGCGGTACAACAACGAAAGGCTTTAACAGTACAATCGTTCGTTTTATTCGGAATCATCAAAAAGACACTTGG
ATTATTTCCGACAACCCTTTGTTAGGTGGAGCTGAATTTGAAGCCTATGAAGGTTGGGTGGGTAAAACCAATTTTAATACC
TATCGCCAAAAAACTTTTGCCATTAATGTCCCAACTAAGTTCTTACAACATTACCAAATTACAAACTGGGAAACTACAACA
GCAGCAGATGGTCAAATCTATGGCACAATTAAATTAGCGAACGGTGCTGAAAAAGTGACCGTTACTCAAGCAGATGCTAAT
TCGCCAAGAAGCATTACGTTAAATGAGACAGAAGTTCTAAAAGGTGATGCGTATCTACTGCCTTGGAATGTCAATGGTCAA
GACAAACTATATCACTGGAATCCAAAAGGCGGTACCAGCACTTGGTCATTGGATAAGAAAATGCAAGGAAAAACGAATTTA
CATTTATATGAATTAACAGATCAAGGGCGTATTGACAAAGGCGCAATTGCCACTACAAATAACCAAGTGACCATCCAAGCC
GAGGCTAATACACCGTATGTCATTGCTGAACCTGACAGTATTGAACCGATGACATTTGGAACAGGAACACCATTTAAAGAT
CCTGGATTTAATGAAGCCAATACCTTAAAAAATAACTGGAAAGTTTTCCGAGGTGATGGAGAGGTTAAAAAAGATGCCAAT
GGTGATTATGTCTTTAGTTCAGAAAAAGAAAGAACCGAAATCAAACAAGATATCAATCTTCCTAAACCAGGAAAATATAGT
TTGTATCTAAACACAGAAACACATGATCGTAAAGCCACAGTAACTGTTAAAATTGGTGGTAAGAAATATACGCGGACAGTG
AATAATTCGGTTGCCCAAAACTACATTCAGGCAGATATTAACCATACAAGCAGGAAAAATCCGCAGTATATGCAAAATATG
CGAATTGATTTTGAAATCCCAGATAATGCCAAAAAAGGCTCGGTGACATTAGCGGTTGATAAAGGCAATTCCGTTACAAAA
TTTGATGATTTACGAATTGTTGAGCGTCAAACGGATATCATGAACCCAGACAAACAAACAGTTATTAAGCAAGATTTTGAA
GACACACAAGCAGTTGGGTTATATCCGTTTGTTAAAGGCTCAGCTGGTGGTGTAGAAGATCCACGGATTCATTTATCAGAA
AGAAATGAACCTTACACACAATATGGTTGGAATGGAAACCTTGTTTCAGATGTATTAGAAGGCAACTGGTCCTTGAAAGCC
CATAAACAAGGAGCAGGATTGATGCTTCAAACAATTCCGCAAAATATTAAATTTGAACCGAACAAGAAATATACGGTCCAA
TTTGATTATCAAACTGATGGTGAAAATGTCTTTACTGCTGGGACCATTAATGGGGAGTTGAAAAATAACAATGACTTTAAG
CCAGTCGGTGAGTTAACTTCGACAGCAGCAGATGGTCAAACCAAGCATTATGAAGCAGAAATAATTGGGGATGCTTCAGGA
AACACTACGTTTGGTATTTTTACAACAGGTGCCGATAAAGATTTCATTATGGATAACTTTACGGTCACAGTGGAATCAAAA
AAA
EF1818
Seq ID 395
TTGATGAAGGGAAATAAAATTTTATACATTTTAGGTACAGGCATCTTTGTTGGAAGTTCATGTCTATTTTCTTCACTTTTT
GTAGCCGCAGAAGAACAAGTTTATTCAGAAAGTGAAGTTTCAACAGTTTTATCGAAGTTGGAAAAGGAGGCAATTTCTGAG
GCAGCTGCTGAACAATATACGGTTGTAGATCGAAAAGAAGATGCGTGGGGGATGAAGCATCTTAAGTTAGAAAAGCAAACG
GAAGGCGTTACTGTTGATTCAGATAATGTGATTATTCATTTAGATAGAAATGGTGCAGTAACAAGTGTTACAGGAAATCCA
GTTGATCAAGTAGTGAAAATTCAATCGGTTGATGCAATCGGTGAAGAAGGAGTTAAAAAAATTATTGCTTCTGATAATCCG
GAAACTAAAGATCTTGTCTTTTTAGCTATTGACAAACGTGTAAATAATGAGGGGCAATTATTTTATAAAGTCAGAGTAACT
TCTTCGCCAACTGGTGACCCCGTATCATTGGTTTATAAAGTGAACGCTACAGATGGAACAATTATGGAAAAACAAGATTTA
ACGGAACATGTCGGTAGTGAAGTAACGTTAAAAAACTCTTTTCAAGTAGCGTTTAATGTGCCAGTTGAAAAAAGCAATACG
GGAATTGCTTTACACGGAACGGATAACACAGGGGTTTACCATGCAGTAGTTGATGGCAAAAATAATTATTCTATTATTCAA
GCACCATCACTAGTAGCATTAAATCAGAATGCTGTTGACGCCTATACGCATGGAAAATTTGTGAAAACATATTATGAAGAT
CATTTCCAACGACACAGTATTGATGATCGAGGGATGCCCATCTTGTCAGTTGTTGATGAACAACATCCAGATGCTTATGAC
AATGCTTTTTGGGATGGAAAAGCAATGCGTTATGGTGAAACAAGTACACCAACAGGAAAAACGTATGCTTCCTCTTTAGAT
GTAGTTGGTCATGAAATGACACATGGTGTAACGGAACATACTGCCGGTTTAGAATATTTAGGACAATCAGGTGCGTTGAAT
GAATCTTATTCTGATTTGATGGGTTATATTATTTCGGGTGCATCTAATCCAGAAATTGGTGCGGATACTCAGAGTGTTGAC
CGAAAAACAGGTATTCGAAATTTACAAACGCCAAGTAAACACGGACAACCAGAAACCATGGCTCAATACGACGATCGAGCA
CGGTATAAAGGAACGCCTTATTATGATCAAGGCGGTGTTCATTATAATAGTGGAATTATTAATCGGATTGGTTACACCATT
ATCCAGAACTTAGGCATTGAAAAAGCACAGACTATTTTCTACAGCTCGTTAGTAAATTACTTAACACCTAAAGCACAATTC
AGTGATGCTCGTGATGCGATGCTTGCTGCTGCAAAAGTTCAATATGGCGATGAAGCAGCTTCAGTGGTATCAGCAGCCTTT
AACTCTGCTGGAATCGGAGCTAAAGAGGACATTCAGGTAAACCAACCAAGTGAATCTGTTCTGGTCAATGAA
EF1850
Seq ID 396
GTGCAACAAAACAACAATGAGATATCTACACAACACCAATTTGATGCCTACTGTAAAAAAGTATTACGCAATGAAGCTAAG
TCTATTCGAAAACGAAACGATAAAATAAAAGAAAATGAGGAACCATTAAATGATTTAAATGAAGGTAAGTATTCACAAACT
CATTTAGATGAGCAAGATGTTTATTTCTTATTTGGAATGGAGATATTAATTTCAGATCAGAAGTTATCAGCAGCAATTGAT
CAGTTATCGGATACAAGGAAAAAGATTGTTTTACTTTATTATTTTGCAGGTTTTAATGATACTGAGATCGGTAAAATTTTC
AACATGAGTACAAGTGGTATCTGGTATCAACGAACGAAAGCGGTTGAACAATTGAAAATGGAATATGGCTTATGG
EF1877
Seq ID 397
ATGAAAAAGAAACTTTTTCGGATTGTGGGAATCGTTTTTATTAGCATACTCACTCTGATAGTTCTATTGAGTTTAGTTGGT
ACGGTAGCCGAGGCGACAGGGCTAGTTGATGATACAGTTGAATCAGGAAATCTATATTCCAAATATTCACTGAACAACTAT
CAACTAGATTTCTTTGTGGATAGCTCTTGGGATTGGTTGCCTTGGAATTGGGGTGATGGCTTAGGGAAAAGCGTGATGTAT
GGTCTTTATGCCATTACTAATTTCATTTGGACGGTGAGTTTGTATTTGTCGAACGCCACGGGTTATGTGGTTCAAGAAGCC
TATAAATTAGATTTCATTTCGGATACGGCTGAAAGTATTGGAAAGAATATCCAAACATTGGCTGATATTACTGAAAATGGA
CTCCAGACCTCCGGATTTTATTTTGGGTTTCTATTATTGATGATTTTAGCTCTGGGGGTGTATGTGGCGTATACCGGATTA
TTAAAACGAGAAACCACGAAAGCAGTTCGAGCCGTGATTAATTTTGTGATGATTTTTCTACTATCGGGTTCGTTCATTGCT
TATGCGCCTACCTATATCACAAAAATCAATGACTTTAGCTCAGATGTTAGTGAAGCGGCCCTTACCCTTGGAACCGAGATC
GTTGTACCCAATTCAGAAAGCCAAGGAAAAGATAGTGTGGATTTGATTCGGGATAGTTTGTTTTCGATTCAAGTCCAACAA
CCTTGGTTACTACTGCAATTTGATGATTCCAACATAGAAGAAATTGGTGAAGACCGGGTCAACAAGATTCTATCTGTGAGT
CCAGATGAAAACAAAGGCAAAGATCGGGAAGAAGCGGTCAAGGCAGAGATTGAAGACAATGACAATGCGAATCTCAGTATT
ACCAAGACCATGAACCGTTTAGTGATCGTGGTCTTTTTGGTATTGTTCAATATTGGCATTTCCTTCTTTGTTTTTCTTCTA
ACAGGGATTATGTTGTTCTCGCAAATTCTCTTCATTATCTTTGCGATGTTTCTGCCAATTAGTTTCTTATTGTCCATGTTG
CCGACTTATGAGAGTTTGGGCAAGATGGCGATTATTCGTTTATTCAATACGATTATGATGCGAGCGGGTGTCACCTTGGTG
ATTACGACTGCATTCAGTATTTCTACGATGTTTTTCAATATTTCGGCTACCTATCCCTTCTTTATGGTAGCTTTTCTACAA
ATTGTGACCTTTGCCGGTATCTATTTCAAATTGGGTGACATTATGAGTATGTTCAACCTTCAGAGTAATGACAGTCAGTCA
ATGGGCAGACGGGTCATGCGAAGACCGCAAATGTTGATGAACCGTAAATTGCGGCAACTCAATCGAAATGTGGGCCGAACT
CTGGCTTTTGGCGGTGCTGCAGCAGTTGGGAATAAGTTGGCAAAGGAACAATCAAAACCCAAATTTAAGCCAGCAGGTTCT
TCTCTACGAAAGAATTCACGATTACCTAACGATCGCGAGGTATCCTCAGACTCGGCTAAGGAGAATCCAATTTCAAATAAT
AAGAAACAATCCCGAATGAATTTGACTGGGAGAAAAATAGGCAAAGTTCTGGATACCCAGGCTTTGGTGAAAGATAAGGCA
AAACAAGTAAAGGATCAGGTACGGAATACGCCGACCAACTTGAAATACATCCTCCATAAAGGTCTGGAAAAGACGAAAAAA
GCACCGAAAGAATTTAAGCGAGGGCTTGTTCAAGAAAAAGCAGATCGAGAGAAGCTGCGAGACAAACAACGACAACGCAGA
GATGAGAAAATGGATGAAAAGCGAAAAAACTTGGGCGAAGTAACAGACCGTCATGGAAAAAGAAGAAGTAATGTTTCGATA
AAAGAGGATCCAAAACCTCAGAAGGATAGGATACTGAAAAATGAACTTCCAAAACGGATTGTGATGGTAAAACCGAATTCT
GAGGTAAGGAGAAGACTTGTTAAACAGAATTTGGTTGCGAGAGAGAATAACCAACTATTAAGTAAGAAAAATTCTTTCCAG
CAAGTTAAGCAACGATCTACTCTTCCGAAGAGAGCCAATCAAAAAGTACAAAGAATAATGAAGGCTCGTCCAAAACCAAAG
TCGGGTGATAAAAAA
EF2174
Seq ID 398
ATGAAAAAAATCATTTCAGGTATGTTAATTTGTACTGTATTATTAAATAGTTTTAGTGTGATTGCATCAGGAGAAGAGCTT
GTAAAAACAGAAACAACGGGGGAGACTGGCTTGGTCACTCAAGCTACGTCAGAAACAACGACTAATTCAACGGAGGATACA
TCTTCTGTTACAGAAGAAAATACCTCTGAAAGAGATAGTTCTACTTCAAGCACAAAAGAAGAGTCTCTTGATTCTTCAACA
AATAGTACAACGGAAGAACATTCTTCTGTTTCAGAAACAAACACAACAGATAGTAAAACAGAAGCATCACAAAGCTCTGAA
GTAGAAAAAAAGACCATTGACCAAGATGAAGCGGATTATCAAGAAGCAGCACGTGAAGGAACGAACCATAAAAAAGGAACA
TATGCTATGAAAAACGGCCTTTCTTCTAGGGTAGCGCGAGCAACAGTTGCGAACGTCTATGCGAATGATCCTAATTTGCCT
GGCAAGAATTTTATTGACGTTTCTAGTTGGAATGGAGACATTTCAGTAGCTGAATATCAAAAAATTAAAAGCTATGGTGTA
ACAGGTGTTTCGGTTAAATTGACAGAAGGAACTTGGTATGTTAATCCATATGCTGCGGGCCAAATTAGAAATGCCAAAGCT
GCGGGCTTAAAAGTTTCAGCATATCACTATAGTATGTATGTCTCTGCTGCGACAGCACAAGATGAAGCAAGATATTTTGCT
CAGGCGGCGGCTAACTCTGGCTTAGATAAAAATACCATAATGTTTAATGATGCGGAAGATCCAACGCTTACAAACAATGGG
CGAAACGCCCATGCTAATTCTGTTGCGTTTAACCAGCAATTAAAGGCATTAGGGTATAAAAATGATGCGTTGTATGTTGGC
AAATGGTGGTTAACAAATGGCTATATCGACACCTCCGCCTTCGGAAGAGACAGAGTATGGGTAGCGCAATATCCATATACT
CCAGATAGTTCCATGCAGTGGAATAATGACCATGGTGCTTGGCAATGGTCTTCGCAAATGTATTTCCCTGGTTTAGCTAAT
TATGAAGGGCGTCCGTTTGATATTTCCATGACCTATAGTAATTTTTTAAATATGGGTAATAGTAGTGGGCCTGACTTAAGT
AAGTATTACACAACTAATCCAGGTCGAGTGATCATGAAAAATGACGATACTTTTTACCAAGATGTAGCGTTTAGAACTCCT
GGTTGGCGAGTTAAAAAAAATACACTAGTTACCATTAAGGGGATTGAATATAGTTCAGCAGGAATTCCTCGATTGGTCACA
GACCAAGGTTATTTAACAGCAAATAAAGATTATGTATTAGCGGCACAAAGCAATATTGATTTGTATTTTACGACAAATCCT
AAAAAGGTTCGTTTGAAAAGCGATGATTATTTCTATGCTGATCCAGAATTTAAGCAAAGGTTGTCTAAGGTTTCGAAGGGA
ACAATAGTAGAAGTTGAAGATTTAGCTTATACTCAAAGCGGTATATTTCGATTGAAAACAGCGAAAGGATATTTGACTGCA
AATAAAAATATAGTTGAACAATATAAAGGATTAGAAGATACTTACTACACATCTAATCCTGGACAAGTAATTACTAGAAAT
GAAGATACCTATTACAAAGATGTAGAATTCAAGACAAAAGCAAATAAAGTATCTGCAGGCAGTGTTTTAAAAGTAACTGCT
ATTGAGAAAACAAAATCAGGAATTCCTAGATTGAAGATGGCAAATGGGTATTATTTCACGGCTAACAAGAACTATGTAGTA
GCTACTGGATCTTGGATTGCTAACTATCATACGGTTAATCCAGGGCAAATTATTATGAAAAATAGTGATAATTTTTATGGG
GATTCAGACTTCTTATATAAAGGAGCAGCTGTTTCCAAGGGAAGTTTAGTTCCTGTTGTGGGAATCGAGTACCGTGAAAAT
CAAGTTCCTCGTTTGATTACCCAAAATGGTTATTTAACGGCTAATAAAAGTTATGCACAAAAAGTTGTGCCAAACATCAAA
GATTATCTATATGATTATCCAGAGTATGTAGTTATGAAAACAAATGATTATTATTATCAAGATGTAAATTTCAGTAAAAAA
GGTGAGTTTGTTTCTAAAGATACATTAATCAAAGTTTTAAGTGTTGATTATACCGAAAACGGATTACCTAGATTAAAGACT
GCCAAAGGTTATATAACTGCTAACAAATCATATGTTACAAAATTGGTATCAAATTCAGATAATTACTTTACTGAAAATCCG
CACCAAATTATTATGCGAGTGTCAGATAAACTATATACTGATGTAGAGTTTCGTAATGGTAGCAGAACCTTATCATCTGGG
ACTGTTGTTCCAGTTAAGGGCATTGAGTATTCCTCTAAAGGTGTTCCTCGCTTGAAGACAGAAGGAGGATATCTTACTGCA
AATAAAAACTATGTGACAGCTGCTGGAAATACCAATAACAATTACTTTATCACTAATCCAAAAAAAGTTAAGCTCTTAATT
GATGATTGTTTCTACAATAATACTGAGTTTACTAAAAAAGGACAGGCTGTTAAGAAGAATACGATTGTTGAAGTTGAAGCA
ATCGAATATACAAACAATGGTATACCAAGGTTAAAGACGAAACAAGGGTATTTAACTGCTAATAAGTGGTATGTAGCAAAA
GTGGGT
EF2224
Seq ID 399
ATGAATAAAGCAGTTAAAAATTTTGTGAGTTACCTTATGATTACCATGCTGTTTATTTTAAATTTGTTACCAATGATGAAC
GCATTTGCTCAAGAAGTAACAAGTGATGCTGAGAAGACTGTTGAAAAAGACGGGCTCAAAGTAATAGGGAAAATAGAAGAT
ACATCGTCTCAAGAAGATATTAAAACGGTCACTTACGAAGTAACAAATACGCGAGATGTACCAATCAAAGATCTCATTTTA
AAACAAAAAAATACCAATGATAGTCCAATCAAATTTGTTTTAGATACTTTAAGTGAAGAGCGAGGACCTACGTCTTTAGAA
GAACAAGCAAAGGTTGAAACAAATGAGAAAGATCAAACAACCGATATTAAACTGCTTAACCTTCAACCAAATTCGACAAGA
AAGATTACCATAAATGGGCAAATAACTACAAAGCGTCGAACAAGTTATTGGTCAGCGTACTAATAGAAGATAATGAAAAAA
GGAACAGTAATTGATTTACCTTTTAGTAAAGATATACTAGCAGATAAAGAGTCGGTTTCGAAGGFAAAACAGGAAACTTCA
GAAACAAAAGTAGAAAATCAGGCAAACGAAACAGCCTCTTCTACAAATGAAATGACCGCAACTACTAGTAACGAAACAAAG
CCTGAAGCAGGAAAGGCGATAGAAAGTATTCAAGAGACAGCACTAACGCAGGCTACCGAAAGTCCTGAGCAACCTCCATTA
AAGGCGCAACCTACTGGTCCGTTAGTGCCACCAACACCTGGTCGGGGGTTTAATACACCGATTTATCAAAGCGTTCATAAA
GGAGAACTTTTTTCAACGGGAAATACCAACTTAAAAATTGCGAATGAAAATACGGCGGCTGCACAAACATTTTTAAACACA
CGAGGAGCAAGTAGTGGTTATGCAATTAACAATTTTCCTTTAGAATTTGCAGATGTTGACAATGATCCAAATACGTATAAC
TCTAGTCGGGCTTATATTGATTTAAATGGTGCAAAAGAGATTGCTTGGGCGGGCTTATTTTGGAGTGCATCTAGATATAAA
GGCCCTGCTTACGGAACAAATCTTTCTGATGAAGAAATTAGTGCACCAGTTCAATTTACTACACCAAATGGAACCGTACAG
CGTGTTTCGCCCCAAAGGTACCATCGTATCGATCAAGATGCAACAAACCCAGGACAACGTTTCGGGTACAATAACACTGGA
TTTTCTAATTATGCAGATGTAACTTCAATTTTACAAGGGGATAAAAGTGCGACAGGGAGTTATACGTTGGCAGATATTCCT
ATGACAAGTAGTTTAAATGGTCAATATCAATATTATAACTTTAGTGGTTGGAGTTTGTTTGTTGTTACAAAGGATCAGGCA
AGTAAGTCAAGAGCTTTTAGTATTTACTATGGAGCACGTGGTAATGCTGCTGGAACCAATAATGAATTTACTATGAGCAAC
TTTTTAACAGCAAAACAAGGAAATCTTGATCCAATTGTGACCTGGTTTACTGTTCAAGGAGATAAATACTGGACTGGAGAC
AACGCACAAATTAAAAATAGCGCAGGAACTTGGGTAAATATTTCGAACACGCTCAATCCAGTTAACAATGCTATGAACGCA
ACTGTGACAGATAACGATGAACATATGGTAGACAAGTATCCAGGGAAATTTGCGCCGGATCATCCTAATTTTTTAGATATT
GATATCGATCGTATGGCAATTCCCGAAGGTGTTTTAAATGCTGGACAAAACCAAATTAATTTTAGAACAACAAGTAGTGGT
GACGATTATTCTACGAATGCGATTGGTTTTGCTGTAAACGCAGAAACGCCTGAATTTGAAATTAAGAAAGAAATTGTGGAA
CCAAAAGAAACTTACAAAGTTGGTGAAACAATCACGTATCGTGTCTCACTAAAAAATACAAAAGCTGATTCTGAAGCGATA
AATTCAGTCTCGAAGGATGCACTAGATGGTCGCTTAAATTATTTGCCAGGCTCTTTAAAAATTATTAGTGGACCAAATTCT
GGTGAAAAAACGGATGCTTCAGGCGATGATCAAGCTGAATATGATGAAACCAATAAACAAATTATAGTCCGTGTCGGCAAT
GGTGCAACTGCTACACAAGGAGGTAGCTACAAAGCTGATACAGCTGAAACAATTTACGAATTTAAAGCGCGGATAAATGAA
CGAGCAAAAGCAAATGAATTAGTGCCTAATAGCGCAACCGTTGAGGCAGTAGATATTTTAACATCTGCAAAAGTCAACGAA
ACTTCTAATATAGTAGAAGCAAAAATTGCGGACGAACAAGTAACTGGCAAATTAACCGCAACAAAAACCGTTAATAACGCC
AAACCAAAACTAGGGGAAGAAATCGAATACACCATCAGCTTCCGCAACACTATCGAAAACGGCATCCTAAACAAAGTAGTT
ATCACAGACCAACTGCCAAAAGGACTGACATATGTCAAAGATAGCCTAACAAGTGTTGGTGATGAACCAAAGCCAACCAGC
TTGAAAGAAACCAACGGCACAATCACAGCGGAATACCCAAGTATCACCGATATGAAAGAACGGAGTATTCGCTTCAAAGTG
ATCGTTAACGAAGAAGCCAAAGCGGGCGAAACAATTCTCAACAAAGCCAAAGTAGATGACACAGTTAATCCACCAGAAGAA
CCAGAGGTGCCTGTGGTACCAGAAACGAACGCAGGCAAACTAGCCGCAACAAAAACCGTTAATAACGCTAAACCAAAACTA
GGCGAAACTATCGAGTACACAATTAGCTTCCGCAATACCATCGAAAACGGCGTCCTAAACAAAGTAGTTATCACAGACCAA
CTACCAAAAGGACTGACGTATGTCAAAGATAGCCTAACAAGTGTCGGTGATGAACCAAAACCAACCAGCTTGAAAGAAATC
AACGGCACAATCACAGCGGAATACCCAAGTATCACTGATACAAAAGAGCGGAGTATTCGTTTCAAAGTGATCGTTAACGAA
GAAGCTAAGGCAGGCGAAACAATTCTCAACAAAGCCAAAGTAGATGACACAGTTAATCCGCCCGAAGAACCAGAGGTGCCT
GTGGTACCAGAAACGAACGTAGGCAAATTAACCGCAACAAAAACCGTTAATAACGCCAAACCAAAATTAGGGGAAGAAATC
GAGTACACAATCAGCTTCCGCAACACCATCGAAAACGGCGTCCTAAACAAAGTAGTTATCACAGACCAACTACCAAAAGGG
CTGACGTATGTCAAAGATAGCCTAACAAGTGTCGGTGATGAACCAAAACCAACCAGCTTGAAAGAAATCAACGGCACAATC
ACAGCGGAATACCCAAGTATCACCGATACAAAAGAGCGGAGTATTCGTTTCAAAGTGATCGTTAACGAAGAAGCCAAGGCA
GGCGAAACAATTCTCAACAAAGCCAAAGTAGATGACACAGTTAATCCGCCCGAAGAACCAGAGGTGCCGGTGGTACCAGAA
GCGAAAGAAGGCAAATTAACAGCAACAAAAACCGTTAATAACGCTAAACCAAAACTAGGGGAAGCGATCGAGTACACAATC
AGTTTCCGCAATACCATCGAAAACGGCGTCCTAAACAAAGTAGTTATCACAGACCAACTACCAAAAGGACTGACGTATGTC
AAAGATAGCCTAACAAGTGTCGGTGATGAACCAAAACCAACCAGCTTGACAGAAGCCAATGGCACAATCACAGCAGAATAC
CCAAGTATCACCGATATGAAAGAACGGAGTATTCGTTTCAAAGTGATCGTTAACGAAGAAGCGAAAGCGGGCGAAACAATT
CTCAACAAAGCCAAAGTAGATGACACAGTTAATCCGCCCGAAGAACCAGAGGTGCCGGTGGTACCAGAAGCGAAAGAAGGC
AAACTAGCCGCAACAAAAACCGTTAATAACGCCAAACCAAAACTAGGCGAAACTATCGAGTACACAATTAGCTTCCGCAAC
ACCATCGAAAATGGTGTCCTAAACAAAGTAGTTATCACAGACCAACTACCAAAAGGACTGACGTATGTCAAAGATAGCCTA
ACAAGTGTCGGTGATGAACCAAAACCAACCAGCTTGAAAGAAGCCAACGGCACAATCACAGCGGAATACCCAAGTATCACC
GATACGAAAGAACGGAGTATTCGTTTCAAAGTGATCGTTAACGATGAAGCGAAAGCGGGCGAAACAATTCTCAACAAAGCC
AAAGTAGGTGATGGCATCAATCCACCAGAAGAACCAGAAGTGCCCATTACGCCTGAAGAACCAGCGAAAAATAAAAAGGAA
ACAAACAAAGTTGTTACAGACCAGAACAAACCTACAAAAAATAGTAAAAATGAAATTGCCATAAACAAGAAAGAAACGTCT
AAATCATCCTATTTACCAAAAACAGGAGAAAAAGTTCAAAAAATATTTGCGTATCTGGGTGTAGGACTGATCTTAATTGTA
TTAATTCTCTATGTTATAAAGAGAAACAAAGAAAAAGAGGAG
EF2318
Seq ID 400
GTGAGCCGGGGAACGGTTTCAGGCACGGGAGAAAAAGTTCAGAAGATGGGCCGGGACGGTCTGGTGGAACAGAACCGCGCC
ACCGGCGAAGAAAAGCGCGTCAGCCAGCGCACGGCAGACACCGCCTTTGGACCGCCCCGCCCTACGGAACGGGAGGACATC
CGCCGCCCGGCAGCACGGGGCGCAGACACCGGCAAAAAGCGGAAACAGCCCCGCACTGCGCCGGAGATGGCGGCAGAACAG
ACGGAACCGCTGCTTACGCCGGAATATCTGGCAGACAGTACTGCCGAACCGCCGCCCGATGCCCCGGCATCCATGCGCGGC
GCGGTGGATATGCCGTTACTGGATGCGCCCATAGCCGAAGAACCCACTCCCGCGCCGGTGAGGAAACAGCCCCAAAAGCGG
GGGAAAAAGGGCAAGAAGAAGCAAAGCACGAAATTCACGGAAGACGCTGCCCGACCGGGTAGTGAGGAAAAAACGGCTATG
CGCGGTGTGGAGGGCGCTCCTGCCCCAAAGCGGCGCACTTCTGAGGGCGGCTCCCGCCTGCAATTTGACAAGGAAGAAGAA
CCGGCAGACCCCGCAGCCGACCGCGCAGCAATCAGAAAGCGGCAGACAGCAAAGCTGGCCGAACACGCCGCCAAACCGGAG
GATAAGCTAAAATCCGATGGCAATCCAAAGGCCGGGGAAACACGCCTGAAATTTGAGGACACTCCCAAAGATACCGACATT
TCCGGCCATACTGCGCCCGATATGCCCACCCGCCAACAAAAGAAATATAACAAGGCCGCCTGCCGCACGGAACGCGCAGAC
CGCCGCGTGGAGCAGGCACAAATGAAGCTGCCTGCCAAACGCCGCCTGCACATGGATCACCAGCCTGACGAAGCCAGCGGG
AAGATAAAGCGGCGGCTCCGTTTTGAGGATGAAATCCTGCCGGAGTACCAAAAGCCCTCCCTGTCCTCCCGTGCCGGAAAT
GCGGCAAAAACGGCGGCAGTCTTAAAGCTGCATGGCAAACTCCGTGAATATGAGCGTGACAACGTGGCTCTGGAATCTGCC
CACAAAATCGAACTGGTTGCCGAACAGGGCGCGGGGCGCGTCCTGcGCTGGGAACGGAACCGCCGCCGCTCCAAACCCTAC
CGCACTTTGCGGAAAGCCCAGCAGAAAGCGGCGCGGGCGCACACTGACCTTGCATGGCAGACCGCCCTGCGGGATAACCCC
GAACTGCAACGCAAAAACGCGCTGGCAAAATGGGTGCAGAAGCAGAAAATCAAGCGGAAATATGCCCAGGCCGCCCATGAA
GCCAAACAGAGCGCGAAATTTACCCAAAACGTCCTGACCGCCACCGGCAAGATTGCCCGCGCCATCGCCCAGTATGCGGCG
GCACATAAGGCGGTCTTTCTGGCGGTTGCAATGCTGGCGCTGGTGGTGATGTTCTTCGCCACGGGCCTGACCTCCTGCACC
GCCATGCTGTCAGGCTTCCAGTCCTCGTACATTTCCGCGTCTTACATGGCTAATGAGCAGGAAATCTGCCAGAGCGACCTT
TATTACACGGAAAAGGAAACGGATTTGCAGATTGATATTGACAAAACTGAGGAAAATTACCCCGGCTATGATGAATACCGC
TACAACATCGGTGAAATCAGCCATAACCCTTATGAACTGTTAGGCTACCTCTCCACGGCGTTCAATGCTTTTACCTTTGCA
GAAGTCCAGCCCGAAATTGACCGCATTTTTTCCCGGCAGTACACCCTCACCCGTGAGGTGATCGTGGAAACCCGGTATGAC
GATGACGGCGACCCTTATGACTGGTATGTTTTGCAGACCACATTGGCCGTGCGCCCGCTGTCCTCTGTGTTACAGAGCAGC
CTCACCCCCGGCGAACAGACCGACCGCTACGGCGTGTATATGCAGACCTACGGCAACCGGCAGGCGTTTGGAAATCCCTTT
GGTTTTTCCTGGCTTGGATATGTGAGCAGCGGCTACGGCTGGCGGGTACACCCTGTAAACGGCGAAAAGAGCCTGCACCGG
GGCATTGACCTCGCTGTGGCGCAGGGGACTCCGATTCTGGCGGCGCAGGACGGGCGTGTGGTATCGGCCGGCGATGCAGGC
AGCTATGGGCTGTGCGTGGTGATCGAGGACGATAAAGGCTACCAGTCCCGGTATGCCCACTGTTCCAGCTTAAACGTCAGC
GCCGGCCAGGAAGTAAAACGCGGGGATGTGATTGCTGCTGTCGGCAGCACCGGCAACAGCACGGGGCCGCATCTCCATCTG
GAAGTCATGTTAAACGGCGAATACTTGAACCCTTATTTCTTCGTGGACAATGGCGACGATGGAACCGGCGCAATCCCCGGC
ACCCCCGGCGGGCCGGCTATCCCGGACTATTCCGGCGAACCGATGGGCGATGGCAGCTTTGAAGCCATGCTGCGGGAAGCG
GAGAAATACCTGGGCTATCCCTATGTGTGGGGCGGCTCCCATCCATCCACTTCCTTTGACTGTTCCGGTTATGTTTCGTGG
GTCATTAACCAGTCCGGCGTGGGCAGCGTCGGGCGGCAGACGGCACAGGGCCTTTACAACCTGTGTACGCCGGTATCTGCG
GTGAACGCACAGCCCGGCGACCTGATTTTCTTTACCGGGACATACAGCTCGCCGGGGCCGGTCAGCCACGTTGGGATTTAT
GTGGGAGGCGGGCGGTTTATTCACTGCGGCGACCCGATTTCCTACGCCAATACGGGAAGCCCCTATTGGTCTGCCCATCTG
TACGGGTATGGCAGAATACCC
EF2704
Seq ID 401
ATGACGAATAAAGAGCCTTGGGAGGCGTGGAGTCCCGCAAAAGTTAGTTCATTCCAGGAAGATTTTTTAGCTTGGTATGAA
CGAGAAAAACGCAATTTACCTTGGCGAGCGAATACAGATGCATATCGTATTTGGATTTCTGAAATTATGCTACAACAAACT
CGCGTAGATACAGTCATTGATTATTTTTATCGATTTATGGAATGGTTTCCGACGATTCAAGATTTAGCGGAAGCGCCAGAT
GATAAGTTGTTGAAAGCTTGGGAAGGGTTAGGTTACTATTCACGAGCGCGTAATTTAAAAGTGGCAGCGCAACAGATTGTT
TCAGAATTTGGTGGGAAAATGCCTGACACAATCGAAGATATTCGGAGTTTAAAAGGAATCGGCCCCTATACGGCTGGTGCG
ATTGGCAGTATTGCCTTTAATCTCCGCGAACCAGCAATTGACGGTAACGTCATGCGGGTAGTGAGCCGTTTATTTGAAATT
GATGCAGATATTGCAAAAGCGAGTAGTCGTAAAGTATTTGAAGCGGCGATGCTTAAAATCATCGATCGTGAGCGTCCAGGG
GATTTTAATCAAGCTTTAATGGATCTAGGCTCAGCCGTCTGCACCCCAACTTCCCCTAAGTGTGAAAGTTGTCCGCTTCAG
CAATATTGTGCGGCCTATCAGGCAGATAAAATGACCGCTTATCCAGTCAAATCAAAAAAAGTTAAGCCGAAAGATGTGTAT
TATGTCGGCACTATTATTGAAAATAAAAAGCAAGAATTTTTATTAGAACAACGCCCTGAAACCGGTTTGTTAGCCAATATG
TGGCTGTTTCCGATTGAAGAAATTAGCAAAAAACAATTTCAACAATTGCAAAAACTGGCGCAACCAGCAGAAACGGAAAAA
CAATTAACTTTAGAGTTAGAGCCTGTAACAGAGCCTTTGGTCGCTGAAGAACCTGTCAACTTTTTTACGGACTATGAAACA
GTTGTCTGGCAAAAACGTACCTTAGGCGAAGTCGTTCATATTTTCAGTCATTTAAAATGGCATATCTTAGTCTTTTATGGC
CGAAATACAGGCGAATTAGCCACACTAGAGTCGCAGCGATGGGTGGCAGCACAACAATTTTCAGACTATGTTTTTCCAAAA
CCACAGCAAAAAATGGTGGAATTGTTCAAAAAAGAACACGAAAAAAAA
EF2713
Seq ID 402
GTGGAAAAGTCAACAGTTACTTCTTTAACTCAAGAAACTTCAGCAACAACAATTAATGCTTCAACTGATTCAACAGCACTT
ACAGCTGAAAGCGAGGAACTTCCATCGCTTCGTCAAACACTTCTTAATTACGTAGGTATGTATGGTTTAACAGAAACCTTG
ATAAATCGTTTATCTGATGACGAACTGGACTACGCAAAAAAAGTTTCGTTTCATTTTGTAAACCAAGACATCAGTGGAACA
GCGAGAATGATTACTAAATTATATGGTGAGAAACCTATCCCGGAGGACTCCTACTCTACAGATTACTCAACATTAACGATT
GATGACTTAAAAAATTATTTACCTCAAATTCGACTTTCGTTAATTTATGTATATGATTTAAATAGTGATGTTGTAAATAAT
TTAAGCGATCAAACACTCGTCGATTTAATTAATCAAGTCAAGGTAGACTACGCTAACCAAAATTACCCATCTGATGTCCGT
GGTGATTATGGTTTAGCAGCAATGGCAGATAAAATAAAAGCAAACGATTATACGTCAATTAATCAAAGTGCTGAAAGTGTT
TCTTCGGATACTACTAATACAGAATCTACATTACAAACAACTACGAGCAGTTCGAAGAAAGCTACTACATCAAGTTCAACC
GAGCACAAAAAGGGGATATTTCCCAGTACTGGCGAAAAAAAATCAGTTTTATTTACTATCATTGGAATCATCTTACTATCT
TTAGTTAGTATATTCATTATAAAAAATAAAAAGAAA
EF2802
Seq ID 403
ATGAAAAAGAAAATTTTAGTAGGAGCGCTTGTCGCTCTATTTTTTATGCCTTTAAATGTATTTGCTGCAAAAGGTGACCAA
GGCGTTGACTGGGCGATTTATCAAGGCGAACAGGGTCGTTTTGGTTATGCACATGATAAATTCGCCATTGCTCAGATTGGC
GGCTACAATGCTAGCGGTATTTATGAACAATACACATATAAAACGCAAGTAGCAAGTGCTATTGCCCAAGGTAAACGTGCG
CATACCTATATTTGGTATGACACTTGGGGAAACATGGACATTGCGAAAACAACAATGGATTACTTTTTGCCACGTATTCAA
ACGCCTAAAAATTCCATCGTTGCATTAGATTTTGAACATGGAGCGTTGGCTAGTGTTCCAGATGGATATGGAGGATATGTA
AGTTCAGATGCCGAAAAAGCAGCAAATACAGAGACAATTTTGTACGGTATGCGCAGAATAAAACAGGCTGGCTATACTCCA
ATGTATTACAGCTATAAGCCATTTACACTAAATCATGTAAACTATCAACAAATCATCAAAGAGTTTCCTAACTCTTTATGG
ATTGCTGCGTATCCTATCGATGGTGTGTCACCATATCCATTGTATGCTTATTTCCCAAGCATGGATGGTATTGGCATTTGG
CAATTCACATCCGCTTATATTGCAGGTGGTTTAGATGGTAACGTAGATTTAACAGGAATTACGGATAGTGGTTATACAGAT
ACAAATAAACCAGAAACGGATACGCCAGCAACAGATGCAGGCGAAGAAATTGAAAAAATACCTAATTCTGATGTTAAAGTT
GGCGATACCGTCAAAGTGAAATTTAGTGTTGATGCTTGGGCAACTGGCGAAGCTATTCCAGATTGGGTTAAAGGAAACAGT
TATAAAGTGCAAGAAGCAACTGGAAGCAGAGTATTGCTAGAAGGCATTTTGTCATGGATTAGCAAAGGTGATATTGAACTA
TTACCAGATGCAACAGTTGTCCCTGATAAGCAACCAGAAACAACACATATAGTTCAACATGGCGAAACATTATCAAGCATT
GCTTACCAATACGGTACCGATTATCAAACTTTAGCTTCGTTAAATGGATTGGCTAATCCAAACCTTATTTACCCTGGACAA
GTTTTGAAAGTAAATGGATCAGCAACAAGTAATGTTTACACAGTCCAGTTGGGTGATAATTTATCAAGTATAGCGGCTAAA
CTTGGCACGACTTATCAAACATTGGCAGCTTTAAACGAATTAGCAAATCCTAACTTGATTTATCCGGGTCAAACGTTGAAC
TAT
EF2813
Seq ID 404
GTGTCAAAACAAGAATCCGATGTTGTCTTAAATTTTAAGATGAATGGAGAAATAAACTATTCACGAACAATTAAAGACATT
AATAAAGAAATGAACTTAGCGGCTACCGAGTACAAAAACCAGGTATCCGCAATGGATAAAAATGCAACTCAAACTGAAAAA
TTAACAGCTACAAAGAAAAAATTAGAAAAACAATTATCTTTAGCTGAACAAAGAACAAAATTATTACGTGAGGAATACGAA
AAATCAGTAAAAGAAACTGGTGAATATTCAGAGCAATCACAAAAGCTGTATAAGCGTTTATTGGAATCCGAAACAGGTGAA
AATAAACTGCGTTCTGCATTGCAAAGTACCAATGAAGCTTTGAAAGAGCAAGGTAATTTATCAATAAAAACAGCTGAAAAA
CTAGCCAAAATTGAAAAAGCTGGAGACAAAATTAAATCAGTTGGGCAAAAACTGTCTGTTGGATTAACAGCACCAATTATG
GGAATTGGTGCTGCTTCTATTGCCGCATTCAAAGAATTAGATGAATGTTTGGATAATATAACAACAGCAACAGGGGCTACT
GGTAGTCAGCTAGAATCTTTACAAGCCAGTTTTAAAACAGTAGCAGGTCAAATACCCGCGGATATGCAAGATATATCAACT
GGTATTGGTGAAGTAAATACTCAATTTGGCTTAATGGATAAGCAATTGGAAGATACAACAGGCCGAATGCTTAAATTTTCA
GAAATTAATGGCTCAGATGTTTCTCAATCAACTATTAATGCAAAAAAATCAATGGACCTTTTTAGGTTGTCTATTGAGGAT
TTGCCAATGATTTTAGATTCAGTATCTAAAACTAGCCAAGATACTGGAGTAGGGGTAGATCAGTTATTTGATGCCGTAAAT
AGAGGTGCGCCCCAACTCAAAGCTATGGGACTTGGTTTTTCTGAATCAACTACGTTAATAGGTCAAATGGAAAAAGCTGGT
ATTGATTCAGCAGGAACTCTTGGCTATTTGGCAAAAGCTAGTGTCGTATATGCGAAAGATAATAAAACCATGCAAGAAGGG
CTTAGCGGAACTATTGAATCTATTAAAGGGGCCACAACTGAACAAGAAAAACTCACTATTGCTAGTGAAGTTTTTGGAACT
AAAGCTGCTTCAAAAATGGTAGAAGCTATTGATAGCGGAGCGTTGTCAATGGATGGTTTAGCAGATTCAGCAAAAAACGCA
GCTGGCACTGTGGATCAGACGTTTAGTGATATTCTTGATCCAATTGACCAAGCAAAGTTGGCACAAAATCAATTTAAAATA
GCAATGGGTGAACTTGGAGAACAAGTACAAATAGCATTATTACCAGCATTTCAAGCTGCAACGGATGCAATAAAAAAAGTT
TCAGAATGGTTTGGAAGTTTAACAGATAGTCAAAAGCAAACCATACTGAAAATAGCTGGTGTTGTGGCTGCTATCGGTCCA
GTATTAGTAGTTTTAGGAACACTTGCTAGTTCCATTAGTAGTTTGATTCCAGTTATTGCTTTTATTGCGTCGCCAATTGGT
TTAGTAATTGCGGCGGTTGCCGCTTGGGTAGCTGCAATCGTAGTTGCATATAATAAAATCGGTTGGTTTAGGGATTTTATC
AATACCTCCTTTAAAGTAATTAAAGATATTGTGGTTGGTGTATTTAATGTTTTGAAAGATACGACAAAATCTACTTTTGAT
TTCATCACAGGATTTATTGGTGGTGCCATGGATGGGGCTGCAAAAATTATTGGCGATTATGTAAATGAAATTAAGCGTATT
TTTGGCGGTATCGTTGATTTTGTAACGGGAGTATTTACTGGAGACTGGTCAAGAGCGTGGCAAGGTGTTGTTGACATTTTT
GGTGGTATTTTTGAAGGCATCGCTGCAGTAGCTAAAGCTCCAATCAATGCCATGATTACGTTAATCAATGGATTTATTGGT
GGATTAAACAATATAAAAATACCTAAATGGGTGCCAGGAATTGGCGGTAAAGGATTTCATATTGGAAAAATCCCTTATTTA
GCAGAAGGTGGAACTATTCTAAATGGCCAAGCCATTGTTGGTGAAGCTGGTCCTGAACTATTAACCGCTAAAAACGGCAAG
ACAACAGTAACTCCATTGTCACCAGAAGAAAAAGCTCGTGGAATTGGTGGTGCTTTGAAAGGTGGCAACACTATTGAGCAA
CATGTTCATATTGGCCAAGTAGATGCAAATAATCCGAGTGAGTTAGATCGAATGAATCGCAAGCTTTATAAAGCAAGTGCG
CAAGCTTTCTATGACTTAGGAGGTGTTCCAACG
EF2820
Seq ID 405
ATGAACAAAGAATTATTGCGTCAATTACAAGCTCGTCACGAGAAACGATTAAGTGATTTACAAGGCAAAATTGAATCTGGA
GAAGTGCGTGAAGCAGATTTAGATTCAGTTAATGAAGAAATTGATGGTTTAATCGATGAATTAAAAGCCATTAAAGCTGAA
TTAGGGGATGATAATTCAGAATCTGGTGATGGTGAAGGCGATGACGGGACCGCTAAATCTGATAATACTGATGATGAAAGT
AAAGAAGATCGTGAGAAAGATACGAACGGAAACAACGATGATAAAAACGAAGAAAATCGTGGCGGCATGATTAGCCAAGAA
CAGCGTGATGGTTTGTTACGCACAATTCATGAAGGAATGGAGGCTAGAAATGTGATGTCTAATGAACAACGTGAAAAACAA
ATTCGTAAAGCATTTGCTGATTTTGTTATTGGTAATATTTCAGAAAGTGAAGCACGTGCATTAGGTATTGAAACAGGCAAT
GGTTCAGTGACAGTACCAGAAGTGATTGCATCCGAAGTGATTTCTTATGCTCAAGAAGAAAACCTATTGCGTAAATACGGA
ACTGTGATTCGCACGGCTGGCGATGTGAAGTATCCAATTCTTGTGAAAAAAGCAGAGGCTAATGTAAACAAAAAAGAACGT
ACGACAGATATTACTGAAACAGCGATTCAATTTGATGAAATTCTACTTGATCCAGCAGAATTTGATGCATTAGCAACTGTA
ACGAAAAAACTATTAAAAATGTCTGGTGTGCCAGTAGAAGATATTGTTGTAGAAGAATTGAAAAAAGCATATGTTCGCAAA
GAAATTAATTATATGTTTAATGGCGACGATGCAGGAAATGAAAACCCAGGAGCTTTAGCTAAAAAAGCTGTTGCATTTGAA
AAACCTGTAGATTTAACAGCTGCAGGTGCTGGTCAAAAATTATATGATGCATTGATTGAATTTAAAAATACACCAGTAACA
GAAGTAATGAAAAAAGGTCGTTTTATTATTAATCGTGCAGCTTTAACTGCTATTGAAAAAATGAAAACAGATGATGGATTC
CCATTGTTACGACCATTCACGCAAGCAGAAGGTGGTATTGGTTATCAATTAGTTGGTTATCCAGTTGATTGGACCGATGCA
GCAGATAAAAAAGGTGAACCAGATACACCAGTATTATATTTTGGTGATTTTTCTGCTTTCAAAATTCAAGAAGTTATTGGA
GCGTTAGAAATTCAAAAATTGGTTGAAAAATTCTCTGGAAAAAATCAAGTTGGTTTCCAAATTTACAACTTGTTAGATGGC
CAATTAGTTTACTCACCATTCGAGCCGGCTGTTTATCGTTATGAAATTACAAAACCAGTAGGTGGT
EF3082
Seq ID 406
ATGAAAAAGAAATTTTTAGCAATGATGGCAGTTTCAATGATGGGACTGTTAATGTTAAGTGCTTGTCAAACAAATAAAAAA
ACAGCAGATTCTGCAACAACAGAAACAACAGCTAAAACGGAAGTCACAGTCAAAGACACCAATGGTCAATTAACCGTTCCC
AAAAATCCTAAGAAAGTCGTTGTTTTTGATAATGGTTCCTTGGATACAATGGATGCACTAGGTGTCGGTGACCGCGTGGTA
GGTGCGCCAACTAAAAATATCCCTGCGTATTTGAAAAAATACCAAAAAGTTGAATCAGCAGGCGGCATTAAAGAACCAGAT
TTAGAAAAAATCAATCAACTAAAACCAGACTTAATTATTATTTCTGGTCGTCAACAAGATTATCAAGAACAATTAAAAGCC
ATTGCGCCAACCATTTACTTAGCTGTAGATGCCAAAAATCCTTGGGCATCAACGAAACAAAATATCGAAACGTTAGGCACT
ATTTTTGATAAAGAAGAGGTAGCTAAAGAAAAAATAACTGGCTTAGAAAAAGAAATTGCTGACGTGAAAAAACAAGCAGAA
GCTAGCGCGAATAATGCGCTTGTTGTGTTAGTTAACGAAGGACAACTTTCCGCTTACGGAAAAGGCTCTCGTTTCGGTTTA
ATTCATGATACATTTGGCTTCAAAGCAGCAGACGATAAGATTGAAGCTTCCACTCATGGGCAAAGTGTTTCTTACGAATAT
GTTTTAGAAAAAAATCCTGGGATTCTCTTTGTGGTAGATCGCACCAAAGCAATTGGTGGCGACGATTCAAAAGATAACGTC
GCTGCAAACGAATTGATTCAAAAAACCGATGCTGGTAAAAATGATAAAGTCATTATGCTTCAACCAGATGTTTGGTATCTA
AGCGGTGGTGGATTAGAATCAATGCATTTGATGATAGAAGATGTTAAAAAAGGATTAGAG
EF3256
Seq ID 407
ATGAAAGTGAATAAATTTGTTAAAGGTTTTGCGGCAATTGCATTATCAAGTTTAGTATTAGCAGCATGTGGTGCAGACAAG
AAAGACAACACAACGAACTCTTCTAGCGCAGCATCTTCAGAAACGAAAAAATCAACGGAATCATCAGCACCAGCGAAAAAA
GTTGCCGGTGGCGATTTAAAAGATGGTACGTATAAATTAGAAGAAAAAAATGAAAAAAATGGTTACCGTGCAGTCTTTGAA
ATGACTGTAAAAGACGGCAAAATCACTGAATCTAAATATGACAACATCAATGCTGACGGCAAGTCTAAAACAGAAGACACT
AAGTATGAAGAAAGCATGAAAGCAAAATCTGGTGTTGGACCAAAAGAATACATCAAACAATTAAACGATTCTTTTGTTAAA
GCACAAAGCGCAAGCGGTGTGGAAGTAGTAACTGGTGCGACTCATTCATCTGAATCATTCCAAAACTACGCACAACAATTA
ATCCAAGCAGCACAAGCTGGTAACACAGACACAATCGAAATCGACAATGGGGCAACATTGAAAGATGGTACGTACTCATTG
AAAGAAAAAAATGACTCAAACGGCTACCACACAACATTCTCAATGACTGTGAAAGATGGTAAAGTGACTGAATCTAACTAC
GATAACGTGAACGCTGACGGCAAATCTAAAAAAGATGACACTGAATACGAAAGCAAAATGAAAGACGTTGCTGGCGTTGGA
CCAAAAGAATATATCGAAACATTAAACAAAGAATTTGTTAAAGCAATGGGCGAAGAAGACGGATCACCTGCAGGTGTTGAA
GTAGTAACTGGTGCAACACACAGCACACATTCATTCATCAACTACGCACAACAATTAGTGAACGCTGCTGAAAAAGGCGAC
ACAACTGAAATCGTTGTTGACAACATCGTAACAAAA
EFA0021
Seq ID 408
ATGGACTTTCAAAATTAACAAACTGTAAAAGAATTAAGATACGCACTGAAACAATACAAACAAACACTCGCAACTACGATT
TTTGATGATACACAAGGCTATATCAGTGTCGCCTTTAAAAAAGAAGTGATAGAAAAAGACGGAAAAAACGTTCAGTTTTTA
TGTTTTTATCCTAATTATGAGGTCGCTCTTTATCCAGTCGAAACGTTACTGGCTTACCTTGAAAAGTTACCTGAACAAGCA
ATCGTTACGATTAAAGACCATGACGGTAACTACTCGTCACTTTTTCTAACAATTGACGAAACGATAGACCAAGGAAACAAC
TACCAATGGCTAGTGATTAGCGACAAAAATTCTTACGAACGATTTTTCTTTACAGCTCAAGAAAGCACGCAAGAACAAGCT
TTTGAGAAAATGTTAAAAGAACTAGAAACCGACCATGACAGTACACTAGAAGTTGTTCATAACTGGTTATGCCGACAAACA
GACGAAGCGCTTTTTCTAGGTATTCTCAAAGAAGATAGAACGTTAAAAGGTGCTATGACTTACTGCATAGGAAAAGCCCAA
GAACAATCCAAAGACCAATCAAGCGCCATGGTGGCGGACGAAGTGGTTTTCAGTTGGATAAAAGAGTATTTCTTATTAGAG
AAATTACCAGAAACCAAAGCAGTTGGTAAAGTAACGACCAAAAAATCAGAAACAAAACCTGAAAGAAAAGAGGTGGACGAA
CAAATTGATTTGTTCGAGTGCGTA
EFA0044
Seq ID 409
ATGATTATTTTATCAGGACTTTTTATTTTAGGGATAGGAATAGTTGGAGGTTATCAGCTAGCCACGTTACCTAAATTGATT
GAAATGAAACAGCACAAAGCAATACAGAACCATTTCAATGTTAAAGGAAATGAGTACACCTATTATCAAGAAGACAGTGAA
AATTACATTTTATCCTTGGAAGATACAGAATATCGAATTAAATTTTCCAAGAACACCCCGTTAAAAGTGGTATTCACTGAA
ATTTTAGAACCTATG
EFA0052
Seq ID 410
ATGAAAAAGATCGCAAGTACAGGGTTAAGTATTTTAGTCGCAACAGGGGTAGCAGGTATTGGGGGAAATGAAGTACAGGCA
GCAGAACAAGCGCAACCAAAAACACCTGAAAACAGTTCTACAGAACAACCAACAGTGAAAGCTACACAAACAACGGAGCAA
GCTATTACTGAAAAACAGCAACAAGTAACAGAGAAACAAGCAATTGTCGATCAAAAACAACAAATCGCTGACACTGCGAAA
AAAGAAAAAGACGCCATTGATCAATCTGTTAAAGACCAACAAGCAGTGGTCGATCAAAACAAAGACGCATTGGATCAAAGT
CAACAAGCAGTGACTGACCAACAAGCAGTTGTAGACGAAGCTAAAAAAGTCGTGGATGAAGCAACACCTTCAGCCATTGAA
AAAACCAAAGAGCAAGTGGCTACGGATACACAGGCAGTTGATGACCAACAAAAAGTAGTAGATCAAGCTCAAACAGACGTT
AACCAACAACAAGCTGTTGTCGAAGAAAAAGCAAAAGAAACGAATGCTGCTAAAGTACAAAATGATAAAGATCAACAAGCA
GTAACAGCTGCGAAACAAGAACAAGCCAAGCTTGAAGAATTAGCGAAAAATGCGGAAGCGGAAAAAGCAAAGGCAGAAAAA
GAACAAGCAGCAAAAGAAGCTGAACTGGCTAACAAACAGAAAGAAGAAGCCAAAGCTAAAGATCAAAAGACGAAGGACGAT
CAAGCAGTCGCAGACCAACAAAGCGTTGTGACAACTAGTCAAGAGAAAGTAGCAGACGCAAAAGCAGATACAGCTGCGAAA
CAGGCAGATTTAACTTCAAAAGAAAATGCTTTAAAAGACAAGCAAGCAGCAACAAAGCAAGCGCAGAATACTTTAGATAGC
TCAAAAGAAGAGTTAAAGGGACATAAAGGACTTAACTTACCTGCTAACTTCTCACCTGATTATTACAAAAAATTAACTGAA
CAAGAAAAGCAAGCAATGGAAAAAGAAGCATTAGCATTAAATAAAGTTTTTCCTGAAAATCAAGCAGATGCGGCAAAAGTA
ACGGAAATGATCAATGTCAAAAATCCTACCGAAAAACAAAAGCAACAAATGAGCGATTACGTTGTAGGACTTATCAATGAT
GTTCGCGAAAAGCTTGGGTTACAAAAGTTGAAGATTTCTAACCAAGCTATGAAATTTGCTTGGGATGTAGCAAAATATGAT
AATCCCAAAGAATTTGATCATGACGTAAATGCGATCAATCGTGCAGCAAAAGAAAATGGTTTTAAAGAATTCCCTGGACAA
AACTTTTATGAAAACCTAAGTATGGGAAGATTTACGACACAAGAAGGTAAAGTTTCTATGTATGACTTTGAAAAAGCTGCT
CGAAATGCACTTGTAAGCATGTTGATGAACGATGGACATTCTGGCTATTCCCATTTAGATTCTTTATTAGATGCAAATGAA
ACAAACATGGCAGTTTCTATTTCAGGAGATTTAAATGATATTTCAGCAAAAATCCATATCATTAGCTATAATCAAACAAAA
TTAGTTGAAGCAAATACCTATGAAGAAGACACTGCACCAGTCTTTAAGAGTAAAGAAACCCTTCAAAAAGAAGTAGCGACC
AATCAAGAAAAATTAGCTACCGCACAACAAGCAGAATCAGACGCACAACAAGCAAGAAGTGCAAGTCAGCAAGCCTTAAAT
ACAGCCAAAACAACACAAGCAACAGCAGAAAAAGAACTATCTGTTCATAAAGCGACATTGGCTAATCTTCAAGCAGTTGCG
ACTAAGAGTACGACAAACTATGAAGAAAAAGTACGACAAACTGCGGCGGCAGAAACAAACCTTCAACAAACAAAAGATCAA
TTGGCAACAATCAATGAGTTAATTCAGAATCGAGCTGTTGTTTTAGAAAAAGCGAAAACAAAAGTTGCAGACGCACAAGCG
ATTGAACAAACGTCTGCTAAAGTGCTGAAAGAAAAACAAGAAGCCCAAAAAGCAGAAGAAAACACATTGAATAGCTTGAAG
GAAGTATTGGATTTAGCAAAAGAAAACTTAAATCAAAAACAAGTTGCGCTTAAAACAAGTACACGTTCATTGGCTCGTTTA
GAAAATGCTCAACCAACATACGAAAAAGCAGTGAACGAGTTAAACAAAGCAGAAGCGGCAGTGGTCCAAGCAAAAGAAGCC
TATGAAAATTCTCTGAAATCATTGGAGGAACTCAAAGAACAACAAGCCGTTGCTACACTTGCTTATACACAAGCACAAGAA
GACCTTTCTAATGCGAAGTTAGAGCTACAGCAGTACCAAGGCATATTAAGAGACTTAGAGGCACAACAAGCCGAACAGCGC
CGACAAGAAGCGTTGCAAGAGCAAGTAGCAAAAGAGCAACAACGCCTTGAACGAGAAGCAAAGCAACAACAAATGTTAGTA
GCAAGTGCTACTTCAGCAGATAAAACACCTGGTCTCCAACAGTTATCTTTTTCTAAACAAAAAGAACAGCCAAAAGCACAA
ACACTAACACATTCAGAACCTCGTAAGACGAAACAAGTAGCAAAAGCCCAAGAATCCTTACCACATACAGGAGAACAAAAA
AGTATCTGGTTGACTATTTTTGGATTATTCATGGCAGTAGGTGCGATCAGTTTCAAGAACAAAAGACGGAAAAATAGT
EFC0004
Seq ID 411
GTGTTGTTAGTATTTGCAAGTTGTGGAGTAAATAAATCAACAGAAGATTCAAAAAAAACAAATGAAACTAAGGTAGAACAA
ATAGCAACTTTATCGGCAGGTACACCAGTTCAAAGTTTAGATCCAGCAACAGCAGTGGATCAAACAAGTGTTACATTGTTG
GCCAACGTAATGGAAGGGTTGTACCGATTAGATGAAAAAAATCAACCACAACCAGCAATTGCAGCTGGGCAACCAAAAATA
TCCAATGGTGGTAAAACATATACAATTGTGATTCGAGACGGTGCAAAATGGGCCGATGGAACAGATATTACTGCTGATGAT
TTTGTAACGGCATGGCAAAGAGTTCTTGATCCTAAAACTGCCTCGCCAAACGTAGAACTGTTTGCTGCTATAAAAAATGCA
AAAGAGATTAGTATCGGAAAGCAACAAAAAGAAACACTTGGTGTAAAATCTAAGGGAAACAAAACAATAGAAATCGAATTA
GAAGAACCTACACCTTATTTTACAGATTTGCTAGCATTGACTGCTTATTTTCCAGTCCAACAAAACGCAGTAAAAGAGTAC
GGTAAAGAATATGGAACAACAAAAGAAAACATTGTAACGAATGGAGCATTTACATTAACTGATTTAAACGGTGTTGGTATT
TCAGATAAATGGACGATTGCTAAAAATCCAAAATACTGGGATAAGAAGCATGTCGCCATGGAAAAAATCAAATTTCAAGTT
GTAAAAGACATTAATACAGGTATTAATCTCTATAATGATGGTCAACTAGACGATGCGCCTGTAGCTGGGGAATATAGTAAG
CAATTAGAAAATAACAAAGATTTCATACGAGAATTATCTGCTACAACTATGTTTTTAGAGGTAAATCAACGAAATAAAAAA
AGTATAACTTCAAATAAACATGCTCGACAAGCGATTAATTTTGCGATTGATCGTGAGGCTATATCAAACAAGATATTAACA
AATGGAAGTATACCAGCTAAAGGTGTAGTACCGAGTAAACTAGTATATAATCCAAAAACAGGAAAAGATTTTACTAATTCA
AGTTTAGTGTTTTTGGATAAGAGTAAAGCTAAAGACAGCTGGGAAAAAACTAAAAAAGAATTAAAAGGTACTGATTTATCT
ATCGATATTATGGTCAATGAAGAAGATTTATCAAAAAAACTAGGTGAATATCTTCAAAATGAATTGCAAGATACTTTGGAT
GGACTTAAAGTTTCAGTTACTGCAGTACCTGCCACGTTACAAACTGAACGATTAAATTCTGGGAACTTTATGATTGCATTG
AGTGGTTGGCAAGCAGACTTTGCCGATCCAGTTAGTTTTTTAGCAAATTTTGAGAGTAAAAGTTCATTAAATCATGGTGGG
TATGCAAACGAAGAGTATGATAAATTGTTGAAAAATAATTCTTCAAAAAGATTGCAAGAATTGAAGGACGCCGAGAAATTA
ATTTTAGAAGATGCGGGTGTAATACCGTTACTTCAAATTGGAAATGCTAAATTAAGAAATCAAAAAATTTCTGAAATGAAA
GTACACTCTATTGGTGCTAAGTATGATTATAAAACCATGGAGATAAAA
EFC0012
Seq ID 412
ATGAAAAAAATTATTTTATCAAGCTTGTTTAGTGCAGTACTAGTATTCGGTGGCGGAAGTATAACAGCATTCGCTGACGAT
TTAGGACCAACAGATCCAGCAACTCCACCAATTACCGAACCAACTGATTCTAGTGAACCTACGAATCCTACTGAGCCGGTG
GATCCTGCAGAACCGCCAGTAATACCAACTGATCCAACAGAACCAAGCAAGCCAACCGAGCCTACAACACCGAGTGAGCCA
GAAAAGCCAACAGAACCAACAACGCCAATTGATCCTGGAACGCCGGTTGAACCGACTGAACCAAGCGAGCCAACAGAACCT
AGTCAACCAACCGAGCCTACAACACCAAGCGAACCAGAAAAACCTGTTACTCCAGAACAACCGAAAGAACCAACTCAACCA
GTGATTCCAGAAAAACCAGCAGAACCAGAAACACCAAAAACTCCTGAACAGCCCACTAAACCAATAGACGTAGTCGTTACA
CCTAGTGGAGAAATTGATAAAACGAATCAATCGGCAGGAACACAACCAAGTATTCCTATTGAAACAAGCAACTTAGCGGAG
GTAACACATGTACCAAGTGAAACTACTCCAATTACAACAGAAGCTGGGGAAGAAATTGTAGCAGTAGATAAAGGTGTTCCG
TTAACCAAAACACCAGAAGGATTAAAACCAATTAGCAGCTCGTATAAGGTTTTACCTAGCGGAAACGTTGAGGTAAAAGCA
AGTGATGGAAAAATGAAAGTATTGCCACATACAGGAGAGAAATTCACACTCCTTTTCTCTGTATTGGGAAGCTTCTTTGTA
TTAATTTCAGGATTCTTTTTCTTTAAAAAGAATAAGAAAAAAGCT
EFC0021
Seq ID 413
ATGGTAGAAAGTAAGCGTACTTGGAAAGAAAAAGTACATGTTGAATCTCAAGTAGAAGAGCGTCTTAGAAAAAATTTGACC
TTAAAATCAAGAGAAGCGACGAAGTTGCAAAAAAAAGCATTAAAGACGACTAAAAAGGAGTTTAAACTTTCTAAGAAAAAT
TACAAGCAATCACTAAAAAAACATAAACTTACCTTGAAAACAGGGAAGGAACCATTAAGTATTAACCATTTACTGGATAAA
AAGAAATTTGTAGAGCAAAAGAAACGCAAAAAAGCACAGAAAATTGCTTACAAACGAACGAAAAAAGTAGATGAAACGCGT
GTCGTTAACCAAGTGAAAAGGGAAACCAAGGAAGGGTTGAAAAGAGAAGCGACGCAAAAAGTACGAACCACGTTAACGCAG
GAAGATACACTGAATGAAGCAATGACCCTTTATGAAAAAACACAACAAGCTAAATTTAACATGCGTACAGCTTTGAAAACA
GGAAAAACGGTTAAGAATCTTTCGGTGAAAACTGCAAAAGATACGTACGGATTGGGGAATCGCTTATTTAATTTTTCAAGA
GGACGTGGGTTTCAGCGCACACCAAAAGACTTTACGTTAAAAAAACAACTTATGAAACAACTACGGAATCGCGCTATGCGC
TTTAAAGCAGCAAAAAGAAGCGAAGAAAGCCGAACAAGGTATTGGCTTGATTCGTTCTTTTTTAATGGTCAGAAGACATTA
GGGAAAGTCGCTGCATTGATTCTAAAAAATCCAATTTCTTGGGTTGTTTTATTGGTTCTTTTTCTTGTTTTTTTATTGAGT
GGCGTTGCAAGTAGTACACAAAAACCAGCGATTGTTCAAGAAGAGGAAGATTTAACCGCTTCATGGACGTATTTTACTAAA
TTAGATGCGCAACATACAGACGATAACAACCTGTTTTATAGCAATATCGATGATGTTCTTTTCTACATGAATTATCGGTAC
GATGATTTTAAATTATTAGATATGGATTCCACTGGTACAAAGAATTTTGAAACGATTCTATCTGAACTTTGGACGGCACTA
AACGGAAAGAAACCTGATTATCAGTTGAAAACAATGCAAAGTTTAGAGACAGATAAGAAATCGAGTTATTTTATCGAAGAG
GAACAAGCAAAGCATTATCAAGAGATAAAAAAAGAGTTAGGCTACCAAACATTGGATGATCTCCTTTCTTTTCCAGTAAAA
ACTGACGCTTTAATTGTCAATAAGCGTTATGGCTATGACAAATCAAAAGAGAAGCTCACCTTATATAAAGGCATTGATGTA
TTGATTGAAGATAATCAGCCGTTTCATTCACCAATGAACGGCCAAATCGTTTCAGTGCCTGATACAGAAACGCTGGTTATC
GAAAAAGAGAAAGTAGCTAGGTTAACGATTCGTGGTGTGAATACCCTTCGACTAACAAAAGGTATGGATGTTGAAGAAGGA
ACATTTCTTGGGAATACAAAGAATTCAACAGTTACGTTCCAATATGAAAAATATAAAAAAGAAACGAAAGACTGGTTTTTT
GTAAATCCAGCCTTTTATTTTCCACGTGTAACCTACACGCAAACGACTCTTTTAGGAAGTGCAGAATTTTCTCCTGGAGCA
AGTGTCGAAAAAAGAGCGCAAGCTGTTTATGATTATTTATCAAAAAGGGATTACACCAAAGAGGGAATTAGCGCGATTTTA
GGCAATTTTTCGGTAGAAAGTGGCATTAATCCAAAACGTGCAGAAGGGGATTATTTGAATCCGCCTGTAGGTGCGCATGGG
AATTCATGGGACGAACCGAGTTGGCTTGCTATGGGAGGACCGCAAATTTATGGAGGACGTTTTCCAAATATTCTCCATCGA
GGACTAGGTTTAGGTCAGTGGACGGATACAGCTGATGGTGGTCGTAGGCATACTTTGTTATTAGACTATGCCAAAGGTAAA
AATAAAAAATGGTACGATCTGCAATTGCAACTGGACTTTATTTTTGACGGCGATGCACCAGGTTCACGAACAGCAGCAGAC
AATGTAGCAAGAAGTAAGGTGGCTGCAACTATTCCAGAACTTACTACTTATTATCTAACTGTTTGGGAAGGAAATCCCGGA
GACAAGTTAGGAGAACGAATTCAAGCTGCACAAAATTGGTTTACATTCTTTTCTCGATCGGGTACACCAATCGGTGGTTCT
GGGAGTGAGGTCTTTGCGCAGTATAAAGAAAAAATGGAACCATTACCCACAGACAGAGAAACAAAAGCAGGACAAGGCTGG
CCAGGCAATGCGTATGCGCCAGGGAATTGTACTTGGTATGTGTTTAATCGTTTTGCACAGTTAGGAAAAAGCATTTACCCT
ACTATGGGAAACGCCAATCAATGGGTGCATAACTACAGTCAAACGCCCGGAGCGACACTTGAATCGGCCCCTAAAAAAGGG
GATGCTGTTATTTTTACAAATGGTGTAGCTGGTTCTAGTACGCAATATGGACACGTGGCTTATGTGGAGCATGTAAACTCT
GATGGTAGTTTTGTCATTTCTGAAATGAATGTCAGTGGTGAGTATTCTATGAATTGGCGTGTCTTAAAAAAAGAAGCAGGC
GAATACTTTATGCGCCTAAAC
EFC0053
Seq ID 414
ATGACCCAAGTACATTTTACACTGAAAAGCGAAGAGATTCAAAGCATTATTGAATATTCTGTAAAGGATGACGTTTCTAAA
AATATTTTAACAACGGTATTTAATCAACTAATGGAAAATCAACGAACAGAATATATTCAAGCAAAAGAATATGAACGAACA
GAAAACCGACAAAGTCAACGAAATGGCTATTATGAGCGCAGCTTTACGACACGTGTAGGCACGCTAGAATTAAAAGTACCC
AGAACACGTGATGGCCATTTTTCACCCACAGTGTTTGAACGTTATCAACGAAACGAAAAAGCCCTCATGGCTTCAATGTTG
GAAATGTATGTATCAGGCGTTTCAACTCGTAAAGTATCAAAAATTGTGGAAGAACTTTGTGGTAAATCCGTCTCTAAGTCC
TTCGTTTCTAGCTTAACAGAACAGCTAGAACCTATGGTTAACGGGTGGCAGAATCGTTTATTATCAGAAAAAAATTATCCT
TACTTAATGACCGATGTACTCTATATAAAAGTACGAGAAGAAAATCGAGTACTCTCAAAAAGCTGTCATATAGCGATTGGA
ATAACCAAAGATGGCGACCGTGAAATTATCGGCTTCATGATTCAAAGTGGCGAAAGCGAAGAGACCTGAACAACATTTTTT
GAATACCTAAAAGAACGCGGTTTACAAGGTACGGAACTCGTTATTTCTGATGCGCACAAAGGATTAGTCTCTGCCATTAGA
AAATCCTTCACCAACGTAAGTTGGCAAAGATGCCAAGTTCACTTCCTAAGAAATATCTTTACCACCATTCCTAAAAAAAAT
TCAAAATCTTTCAGAGAAGCTGTTAAAGGAATTTTTAAGTTCACAGATATTAACTTAGCGCGTGAGGCTAAAAATCGATTG
ATTCATGATTATATCGATCAACCAAAATATTCAAAAGCTTGCGCATCATTGGATGATGGATTCGAAGACGCCTTTCAATAT
ACCGTACAAGGAAATTCCCACAATCGACTAAAGAGTACCAATCTAATTGAACGACTGAATCAAGAAGTACGCAGAAGAGAA
AAGATTATTCGCATCTTCCCCAATCAAACATCAGCCAATCGCTTAATTGGAGCCGTTCTTATGGACCTACATGATGAATGG
ATTTATTCTTCAAGAAAATACATCAATTTTGATAAG
ARFC0021.1
Seq ID 415
ccaagtgaaaagggaaaccaaggaagggttgaaaagagaagcgacgcaaaaagtacgaaccacgttaacgcaggaagatac
actgaa
ARFC0021.2
Seq ID 416
gagaacgaattcaagctgcacaaaattggtttacattcttttctcgatcgggtacaccaatcggtggttctgggagtgagg
tctttgcgcagtataaagaaaaaatgcaaccattacccacagacagagaaacaaaagcaggacaaggctggccaggcaatg
cgtatgcgccagggaattgtacttggtatgtgtttaatcgttttgcacagt
ARF0324
Seq ID 417
ctcaataaaagggcttgcgaaagtagaagctgtttacgatcaagaaatccaagaggtaagagaaacgtacaatgcgattat
tgcaaatgtagaaagagtgcctgc
ARF1627
Seq ID 418
gtgcctacattgcttacaaaccaactgtcggtatgccagaagcacggcggacagttatttcaaatattcacaaaggtggga
caccggctgttgaagcaggggcctacattgccgaaattattaaaaaaatgt
ARF1650
Seq ID 419
aagcggctgcaaaaaaagctgaaaaagtctatctcgcagccgatccggaccgagaaggtgaagcaattgcttggcatt
CRF0097
Seq ID 420
atggcattgttacctaatgtcacaagtaaaccagcccacggtgttgccattcctgtcacaggtacttttaagccaaagaag
gtcactacaatgccactaaccattcctgaaaataaattcgttgcg
CRF0257
Seq ID 421
agttgcgcattttgccacgtttctttcgttggcaaatattttcgtagtccagctactggtacgaccacgattcctgcattc
gctgtcatcaggaaatttaacgcactcactcgatctgcgcgcgcttctggtgaagaaaaggccatttcggcggataacact
tcatctactggaaataga
CRF0635
Seq ID 422
ggaagcatcgtagataattcaccagatatcaatgcgattggaccagcaaagaataagaccgcgaaaattgtg
CRF1152
Seq ID 423
agccaggggcaatgttatggccaattaatgctgcttcaatacaaattgttccagaaccacaaactggatcataaaaaggac
gatctttgcgccaattggtcaacatcactaaagcagctgccatgttttcct
CRF1720
Seq ID 424
Tcattcgtccagtcctcctactgttcttcgctacgtgcgttttccggtaaaattaaattcaaaacaattcctaagacagtt
gctaatgccattgaagacaattcaaaagttcctactttgaagactaagccaccaatgccgatgactaaaattactgaagca
atcaataaattctttttcttatcaaaattaatcttattatcgattaagattttcaaaccacttgcggcaatcacaccgaac
aagacgaaactaattcctgaaataactggtcctgggatacttagaatcaaggcacttaattttccaacaaagcctaaagct
acagcgaacaccgcggctccaccaatcacgaagacactgtgtactcttgtaattgctagcacgccgatattttcacca
EF0008
Seq ID 425
MINNVVLVGRLTKDPDLRYTASGSAVATFTLAVNRNFTNQNGDREADFINCVIWRKPAETMANYARKGTLLGVVGRIQTRN
YENQQGQRVYVTEVVCENFQLLESRSASEQRGTGGGSFNNNENGYQSQNRSFGNNNASSGFNNNNNSFNPSSSQSQNNNGM
PDFDKDSDPFGGSGSSIDISDDDLPF
EF0028
Seq ID 426
MAVKKKKATFWEFFQGLGKTFMLPVALLAFMGILLGLGSSFSSESMIETIPFLGKPAVKIIFQFMSTIGGFAFAYLPVMFA
MAIPLGLVRKEKGIAAFSGFVGYTVMNLAINFYLVQTNRLVDPEQLREAGQGMVFGIQTIEMGVLGGIIAGLIVYKLHNRF
YTVQLPDSFAFFSGARFVPIITSLVMAFVGLVIPLVWPLFALMIMAIGQLIQRSGIFGPFLFGSGERLLLPFGLHHILVSM
IRFTEAGGSAVVAGKEVFGALNIFYAELQNNLPISPSATAFLSQGKMPTFIFGLPAASLAMYHTAAPANRHKIKGLLLSGV
IATAITGITEPIEFLFLFISPLLWLFHVIMTGLGFMVMALLGVVIGNTDGGLLDFVIFGLLQGTYTKWWWVLIVGAIWFVV
YYFVFKTVIVTFDLKTPGRDKVLDETEYTDQEVQYKKTGGYDAPGILAALGGQENIQAIDNCITRLRLVLADANKVDDDKL
KQLGALGVVHLDAQNVQVIIGTKVTTVRNQLEMILG
EF0146
Seq ID 427
MKKIASAGLSILVATGVAGIGGNEVQAAEQAQPKTPENSSTEQPAVKATETTEQAITEKQQQVTEKQAIVDQKQQVADTAK
KEKDTIDQSVKDQQAVVDQNKGALDQSQQAVTDQQAVVDEAKKVVDEATPSAIEKAKNQVATDTQAVDDQQKVVDQAQADV
NQQQAVVEEKAKETNAAKVQNDKDQQAVTAAKQEQAKLEELAKVAEVEKAKAEKEQAAKEAELANKQKEEAKAKDQKTKDD
QAVADQQTVVTTSQEKVADAKADTAAKQADLTAKENALKDKQTATKQVQNTLDKSKEELKGHKGINLPANFTPDYYKKLSE
QEKQAMEKEALALVKVFPENQADVAKAYEMINVKNPTGKQKQQMSDYVVGLINDVREKLGLQKLKISNQAMRFAWDVADYD
NPKKFDHDVNAINRAAKENGFKEYPGQNFYENLSMGYFETINGTISQLEFEKAARKTIADMLFDDESSAYSHIDSLLKGDT
TNMAVSISGDLNDISAKIHIISYNQSKLVEANTYEEGTAPVFKSKETLQKEVATNQEKLATAQQAESDAQQAKSASQQVLN
TAKTTQATAEKELSVHKATLASLQAVATKSTTNYEEKVRQTATAEKSLQQTKDQLATINELIQNRAAVLEKAKTNVSEAQA
IEQTSAKVLEEKQAAQKAEENTLNSLKEVLNLAKENLNQKQVAFKTSTRSLSRLENAQPTYEKALNELNKAEAAAVQAQEA
YENSLKSLEELKEQQAVATLAYTQAQEDLSNAKLELQQYQGVLRELEAQQAEQQRQEALQEQVAKEQQRLEREAKQSQTLV
ASATSADKTPGLQQLSFSKQKEQPKAQALTHSESRKTEQVAKAPDSLPHTGEKNNKWLAIAGLIFALLGAAGIISFISRNE
KKVKNIFKIK
EF0153
Seq ID 428
MKKMIIIALFSTSLLAGGSSVSAYAQESEGNLGETTGSVLPDEPNVPTDPITPSEPEQPTEPSTPEQPSEPSTPTEPSEPS
KPTDPSLPDEPSVPTEPTTPSKPEQPTEPTTPSVPEQPTEPSVPEKPVEPNKPTEPEKPVPVVPEKPVVPQQPEQPTDVVV
KPNGEIATGESTQQPTVPIETNNLSEVTHVPTVTTPIETASGEAIVAVDKGVPLTQTADGLKPIKSEYKVLPSGNVQVKSA
DGKMKVLPYTGEKMGIIGSIAGVCLTVLSGILIYKKRKV
EF0394
Seq ID 429
VKKRLFASVLLCSLTLSAIATPSIALADNVDKKIEEKNQEISSLKAKQGDLASQVSSLEAEVSSVFDESMALREQKQTLKA
KSEQLQQEITNLNQRIEKRNEAIKNQARDVQVNGQSTTMLDAVLDADSVADAISRVQAVSTIVSANNDLMQQQKEDKQAVV
DKKAENEKKVKQLEATEAELETKRQDLLSKQSELNVMKASLALEQSSAESSKAGLEKQKAAAEAEQARLAAEQKAAAEKAK
QAAAKPAKAEVKAEAPVASSSTTEAQAPASSSSATESSTQQTTETTTPSTDNSATENTGSSSSEQPVQPTTPSDNGNNGGQ
TGGGTVTPTPEPTPAPSADPTINALNVLRQSLGLRPVVWDAGLAASATARAAQVEAGGIPNDHWSRGDEVIAIMWAPGNSV
IMAWYNETNMVTASGSGHRDWEINPGITRVGFGYSGSTIVGHSA
EF0443
Seq ID 430
MKSIKTILLGTTLAAGLGLFLGTDANAESLYTVKAGDTLSTISHQFAGDNSLIQKIASDNKLPNLDLIFEGEQLVIRSEKE
VANTPAPAVEVAPVQQVVEQPVAQPVQQEVQQPVAQEVAQPAAPAASSDAKEWIAQRESSGSYDATNGQYIGRYQLSASYL
NGDYSPANQERVADQYVAGRYGSWDAAKSFWLANGWY
EF0568
Seq ID 431
MKKIYQWAVGQSFKKLDPRQQVKNPVMFVVYLGALITTILCFYPMGIPLWFNISITIFLWLTLLFANFAEAVAEGRGKAQA
DSLKQAKKEVMTYKINSLEDIKEENFIELQSSDLKRNDLVYVRAGEQIPADGDVIEGAASVDESAITGESAPVIRESGGDR
SAVTQGTTVVSDYLVIRVTSENGQSFLDKMIAMVEGTQRKKTPNEIGLQIFLITLTIIFLTVSITLVPFTDFSSQLSGKGE
ALSLVIVIALLICLAPTTIGALISSIGIAGMSRLTKENVIAMSGRAIEAAGDVDVLLLDKTGTITLGNRRASDFLPVHGVS
EEQLADAAQLSSLADETAEGRSIVILAKERFNLREREFQQSEVKFIDFSAKTRMSGIDYRGDVIRKGAADTMKKYVQSKGE
DYPSECDKIVDKIARAGGTPLVVIKNNRVMGVVYLKDIVKNGVKEKFADMRKMGIKTIMITGDNPLTAAAIAAEAGVDDFL
AEATPENKMNLIREYQEKGHLVAMTGDGTNDAPALAQADVAMAMNTGTQAAKEAGNMIDLDSSPTKLLQVVQIGKQLLMTR
GALTTFSIANDIAKYFAVIPVLFYSIYPQLDRLNIMGLGSPLTAILSAVIYNAVVIVALIPLALKGVRYQEKPASQILSHN
LLIYGLGGIIAPFIFIKIIDLILSLIIL
EF0591
Seq ID 432
MFFTGKERNLKFAIKKKHGGGGAASVIIGCIIFGSIAFGGTTLAYADEVHNSINQDIQDSGSTIIGENDSSTKSAEYKMIH
EIDGTKISNGENSKETTTSSGTILAEEAIESSNQKNSKTSEVEQDLHKDVSGSESVKQVETSDSIKKSEESAVKTLNLDDS
QENTNSITTKAENDALSTVNDEKVLNESDSIIKSIPSETENVDNNSKSDNRDLVENSVETTKEIMKEADKTEIAKRNDNIS
VLNAGTESFNDSDKIRRSKRDNESPVPSLSIQMPVTKEIYENDSTIKGKSEPGVTIKIYKNGIELGSIKADDDGNFEYPLE
SQAIKSDNYSFVAEKDGAKSIESSVNVVENKEIVGNQRDAYTPYGFGIYNRGLVTTDDGKKHYTVDMEGNLASEAYFDLAG
GTMYYSIDEKFAPYVEKIVLDGKTIISKNPYELPNKTNIWSSGILSTDKRGGLVRAALVGRTNGTIDIYFKDDTPQEVLNS
EIPFQVWARFKEKKEGVKNDLISDFDLNILLQNNKLILSKDTFFKDEDIVNNNGPKISSNFNYTNNTIDINYGVNTGNYGL
TIGKRSPYNLHINTGKELSDLVDTIKIDNKNYEFEKLPDGSLVIKDIYKKGILGGALLNRQEITISLGLIAKKNLGDLIDF
KQELLKLDVSIRDNTTFKLVKGEATEAIRKIDSSFDTFKQELTKWIEKRDSNPQEPITINSGDNTSIVDPKEMVEFAVKNP
TTENINKARSLVSKMWLWDKNKYNPVLNAAEEVNRYLSTTLNGSSNDDGLILNNTHAATFVKLIDTDKDGILDRYEANMGI
GTNYTNADSDGDGKSDGFEIFNDTDPLVSPYDWFDKNGEKIKIVTTDTDTISGRIGNNNYDTENVYPRTVQLIKVTDNGEN
LISEISSSEDRKGTFEFTGLSGKLSKGDRLVVKIITNEVQREIDRQRNVVQVGYDNPEVSEEVIVQGAQVTVSFDFNTNQT
DPDFDRQPVTQVVELEKGSRLLNSYFTPERKGYKFIGWNTDKLGVGQTVNDGSSFNEDITVFAQWEKEPNLAGEVHAPQGP
IEEGKSIVLNNVVQSNKSGSVTVTGELPKGLFGLSINETGNLIGTPLINDWIDGENSREVKIPVTISNGDEKVMVEVPLTI
LHDTRAADAVKAAEDAGKAGADKKAEVETDGLVTPEEKAAVDGLNDTTTAKKEDASKLVDALPEGPVKDSLKDRLDKVTTS
EVTVNDADSNGKADDVDLAEKAAADAVKAAEDAGKAGADKKAEVETDGLVTPEEKAAVDGLNDTTTAKKEDASKLVDALPE
GPVKDSLKDRLDKVTTSEVTVNDADSNGKADDVDLAEKAAADAVKAAEDAGKAGADKKAEVETDGLVTPEEKAAVDGLNDT
TTAKKEDASKLVDALPEGPVKDSLKDRLDKVTTSEVTVNDADSNGKADDVDLAEKAAADAVKAAEDAGKAGADKKAEVETD
GLVTPEEKAAVDGLNDTTTAKKEDASKLVDALPEGPVKDSLKDRLDKVTTSEVTVNDADSNGKADDVDLAEKAAADAVKAA
EDAGKAGADKKAEVETDGLVTPEEKAAVMA
EF0592
Seq ID 433
LPEGPVKDSLKDRLDKVTTSEVTVNDADSNGKADDVDLAEKAAADAVKAAEDAKKAGADKKAEVETDGLVTPEEKAAVDGL
NDTTTAKKEDASKLVDALPEGPVKDSLKDRLDKVTTSEVTVNDADSNGKADDVDLAEKAAADAVKAAEDAGKAGADKKAEV
ETDGLVTPEEKAAVDGLNDTTTAKKEDASKLVDALPEGPVKDSLKDRLDKVTTSEVTVNDADSNGKADDVDLAEKAAADAV
KAAEDAGKAGADKKAEVETDGLVTPEEKAAVDGLNDTTTAKEDASKLVDALPEGPVKDDSLKDRLDKVTTSEVTVNDADSN
GKADDVDLAEKAAADAVKAAEDAGKAGADKKAEVETDGLVTPEEKAAVDGLNDTTTAKKEDASKLVDALPEGPVKDSLKDR
LDKVTTSEVTVNDADSNGKADDVDLAEKAAADAVKAAEDAGKAGADKKAEVETDGLVTPEEKAAVDGLLEIKQSSFMPFEN
LFSTTNDYSQFPKTGEKSDSILTIYGGLLFLSSIGLLGIKKRKNNTN
EF0658
Seq ID 434
MKPKINYTHIAEPTVFENVFPTYLTNETMMARKQKVLQRMETEKFDQLVFYADKEHGSNFEYLTGFIPRFEEGLLVLNKDG
AATLILGNENLKLCQHARISADLIHYLAFSLPNQPMAGEQKLSQIFETLLDETAQKIGIVGWKMFTTQQQEPATMFDVPHF
IVEALKKALPKEARLLNGTHLLIGAKGARATNNANELAHYEYGANLASRSMLKALNAIEIGQRETDIGALLNDEGQTPTVV
TIAATGQRFEYANMYPTAKEIQLGDALSLTTGYKGGLSSRTGFVIENEQQLPEAQRDYLERVAKPYFQAVVHWLETIRIGL
LGREMYQAIEEQLPKEIYHWHLNPGHLVSDDEWMSSPIYPDSAIRLESGMLFQVDIIPSVPGYTGVSAEECVALADETLQK
EIQQTYPDMWQRIATRKAYLKETLKLDLPSEVLPMSNLVGYLRPFYLAKDKALCVEKPALK
EF0727
Seq ID 43S
MKKARVIYNPTSGKELIKKNLADILSILEECGYEASAFATTPEENSARNEAHRAARAGFDLLVAAGGDGTINEVVNGIAPL
KRRPKMAIIPAGTTNDYARALKIPRDNIVKAAEVIKKNQTVKMDIGQAGKNYFINIAAGGHLTELTYEVPSELKSIFGYLA
YLAKGAEMLPRVKPIKMRMTYDEGVYEGNASMFFLGLTNSVGGFEQIVPDAKLDDGKFSLIIVKTANIFEILHLVALMLNG
GKHVEDHRLIYTKTSYLHAETLEKNNKMMINLDGEYGGDAPMTFKNMHQHIEIFANGDALPSNAIMGSVLTGSDEIVVESE
DEEEEAYNEASKEFVKEVERLTDEDIDGDGKIAEKEKH
EF0775
Seq ID 436
MTNTVKVKDDSLADCKRILEGQATEPVQAGETEPVDLVVVEDASGSFSDNFPHVRQAIDEVVQGLSDQDRVMLASYRGGKQ
FMFPDGKTKINSADYDMNVRVNTQLTYDKSQFVSGFGDVRTYGGTPTAPGLKLALDTYNQTHGDLTNRKTYFLLVTDGVAN
TRLDGYLHKTNTNDSINEYPDPRHPLQVSVEYSNDYQGAAAEVLALNQEITNQGYEMINAYWESVESLSSVNSYFDKYKTE
VGPFVKQELQQGSSTPEDFITSQSIDDFTTQLKQIVKDRLAQSTPATASLTIANQFDIQSATATDDAGNDVPVQINGQTIS
ATSTEGYVGNITIHYEVKENTAIDAATLVSSGTMNQGTIAKEFPEATIPKNDNAHACDVTPEDPTITKDIENQEHLDLTNR
EDSFDWHVKTAFGNETSTWTQASMVDDINKVLDIIDVKVTDENGKDVTANGTVTQENNKVTFEMNKQADSYDYLSGHTYTM
TITTKIKTDATDEELAPYIEQGGIPNQADLNFGNEGDVLHSNKPTVTPPPVDPNIAKDVEGQEHLDLTNRDQEFKWNVKTA
FGNETSTWTQASMVDDINKVLDITDVKVTDENGKDVTANGKVTQENNKVTFEMNKQADSYDYLSGHTYTMTITTKIKASAT
DEELAPYIEQGGIPNQADLNFGNEGDVLHSNKPTVTPPAPTPEDPTITKDIEGQEHLDLTNRDQEFKWNVKTAFGNETSTW
TQASMVDDINKVLDITDVKVTDENGKDVTDNGIVTQENNKVTFTMNKKDDSYSYLAHHTYTMTITTKIKTDATDEELAPYI
EQGGIPNQADLNFGNEGDVLHSNKPTVTPPAPTPEDPKKPEPKQPLKPKKPLTPTNHQAPTNPVNFGKSASKGIHLPMTNT
TVNPLYMIAGLIVLIVAISFGITKNKKRKN
EF0779
Seq ID 437
MSAWQTFKNGTRQFFKDILQYLWLFFTLNVLLLLVGGAFSWATSNALKTQGIPYLSFNNLNLLLEKPLALVLLILLLLLFL
GAVFYQFTFLLLGIFQIRQDHRFHFKGVTKASFKVLKKQGARSWLFFFGYFVVIVPFGNLIFQSNLLTKFVIPDFIVEFLS
QRIPYLVGLLAFGLLVWYLAIRFIYTLPLMILERKKAGEAVKASWSMTNKRLWFIIRNIAFVTIAVFVSTYVIYVLLYLLQ
LKLDTLSDTISLLGGILNLTVVQFLQFVSNAWLSVLLINFLYTQLNVQAETTTKVAFDKETKRNKLVTIGMGLGLFTIFGG
YIIFNAVYLTGLLESKPLIISHRGVTNSNGVQNTIPAMERTIKFKPDYIEIDVQETKDHQFVVMHDANLQELAGVDGTPQE
FTLAELTKMTVKENGQEAPIASFDDYLAKANQAKQKLLVEIKTSKQDSQGALSNFIEKYERPLIKNNHQVQSLDYNVIKAF
KKAKSKVKVSFILPYNFTFPETQADLYTMEATTLNDTFILKADQQKKAVYAWTVNDSEVLSKMLFMDVAGVITDDLELVNE
EVNDFEKNPSYADRILHYIFMLPSVASQ
EF1091
Seq ID 438
MITDENDKTNINIELNLLNQTEQPLQREIQLKNAQFMDTAVIEKDGYSYQVTNGTLYLTLDAQVKKPVQLSLAVEQSSLQT
AQPPKLLYENNEYDVSVTSEKITVEDSAKESTEPEKITVPENTKETNKNDSAPEKTEQPTATEEVTNPFAEARMAPATLRA
NLALPLIAPQYTTDNSGTYPTANWQPTGNQNVLNHQGNKDGSAQWDGQTSVNGDPTNRTNSYIEYGGTGDQADYAIRKYAR
ETTTPGLFDVYLNVRGNVQKEITPLDLVLVVDWSGSMNENNRIGEVQKGVNRFVDTLADSGITNNINMGYVGYSSDGYNNN
AIQMGPFDTVKNPIKNITPSSTRGGTFTQKALRDAGDMLATPNGHKKVIVLLTDGVPTFSYKVSRVQTEADGRFYGTQFTN
RQDQPGSTSYISGSYNAPDQNNINKRINSTFIATIGEAMVLKQRGIEIHGLGIQLQSDPRANLSKQQVEDKMREMVSADEN
GDLYYESADYAPDISDYLAKKAVQISGTVVNGKVVDPIAEPFKYEPNTLSMKSVGPVQVQTLPEVSLTGATINSNEIYLGK
GQEIQIHYQVRIQTESENFKPDFWYQMNGRTTFQPLATAPEKVDFGVPSGKAPGVKLNVKKIWEEYDQDPTSRPDNVIYEI
SRKQVTDTANWQTGYIKLSKPENDTSNSWERKNVTQLSKTADESYQEVLGLPQYNNQGQAFNYQTTRELAVPGYSQEKIDD
TTWKNTKQFKPLDLKVIKNSSSGEKNLVGAVFELSGKNVQTTLVDNKDGSYSLPKDVRLQKGERYTLTEVKAPAGHELGKK
TTWQIEVSEQGKVSIDGQEVTTTNQVIPLEIENKFSSLPIRIRKYTMQNGKQVNLAEATFALQRKNAQGSYQTVATQKTDT
TGLSYFKISEPGEYRMVEQSGPLGYDTLAGNYEFTVDKYGKIHYAGKNIEENAPEWTLTHQNNLKPFDLTVNKKADNQTPL
KGAKFRLTGPDTDIELPKDGKETDTFVFENLKPGKYVLTETFTPEGYQGLKEPIELIIREDGSVTIDGEKVADVLISGEKN
NQITLDVTNQAKVPLPETGGIGRLWFYLIAISTFVIAGVYLFIRRPEGSV
EF1323
Seq ID 439
MGILLPIIIAVLVLLMLLIVFVSKYQTAKPDEALIISGSYLGSKNVHVDEGGNKIKIVRGGGAFVLPVFQRSNRISLLSSK
LDVSTPEVYTEQGVPVMCDGTSIIKIGSSVEEIATAAEQFLGKTTEELENEAREVLEGHLRSILGSMTVEEIYQNRDFSQS
VQEVASVDLAKMGLVIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAEALKETRIKKAEAEKESQQAELQRQTEIAE
ASKEKELKLALYKQEQDIAKAKADQAYNLESARAQQHVVEQEMEVKVVERQKQIELEEKEITRREKQYDSEVKKKADADRY
AREQEALAQKAREVAEAEAERFKVEALAEAEANKTRLTGQAQAEAILARGAAEAEAKQKIADAFKEYGEAAVLSMVMEMLP
QLMKEAAQPLGNIDKISVVDTGAGGENSGANRITNYATNLLAGTQETLKETTGLDVKELIENFSKKGTSNSVNYHATEGSE
KE
EF1355
Seq ID 440
MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKSVEEIPSPVTGTVKNIVVPEGTVANVGDVLIEIDAPGH
EDNDAAPAAPAQEQTPAQPAAVPTTEAAGGFFQFKLPDIGEGIAEGEIVKWFVKAGDTINEDDSLLEVQNDKSVEEIPSPV
TGTVKNIVVPEGTVANVGDVLVEIDAPGHNSAAPSVAAPATDAPKAEASAPAASTGVVAAADPNKRVLAMPSVRQYAREKD
VDITQVTATGKGGRVIKADIDAFVSGGSQAAPATEAAATEAAPKAEAAAPKAAPKAFTSDLGEMETREKMTPTRKAIAKAM
VNSKHTAPHVTLHDEVEVSKLWDHRKKFKDVAAANGTKLTFLPYVVKALTSTVQKFPILNASIDDAAQEIVYKNYFNIGIA
TDTDHGLYVPNVKNANTKSMFAIADEINEKAALAIEGKLTAQDMRDGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQ
EPVVNADGEIVVGRMMKLSLSFDHRIVDGATAQKAMNNIKRLLADPELLLMEG
EF1699
Seq ID 441
MWYTIKKMASLSGVSARTLRYYDEIGLLQPARINSSGYRIYGQAEVNRLQQILFYRELDLKLDEIKEILEQPDFNVEQALY
EHQQKLLEKRNEIDRLLASVQQTLHHYKGEINMSDQQKFEAFKQQKVQENEEKYGKEIREKYGNETIEQANKKYLNLTEKD
MQAMQNVEKDLFSKLAMYQKSPKLTSQLAQEIFQLHKDWLMYSWSSYSPEAHKGLGLMYVGDERPTSYYEQHGAGFAEALN
AIIQNYA
EF1744
Seq ID 442
MAKKGGFFLGAVIGGTAAAVAALLLAPKSGKELRDDLSNQTDDLKNKAQDYTDYAVQKGTELTEIAKQKAGVLSDQASDLA
GSVKEKTKDSLDKAQGVSGDMLDNFKKQTGDLSDQFKKAADDAQDHAEDLGEIAEDAAEDIYIDVKDSAAAAKETVSAGVD
EAKETTKDVPEKAAEAKEDVKDAAKDVKKEFKG
EF1752
Seq ID 443
MKRKLTKSPNNVVLTGSLAGIADWLGIDPTIIRVVYVLLSFFSAGFPGILLYIALAVLIPSGRTGSDRGYGHQNPYNRNVR
NENPYAANKKQRKEAEKIDDDEWSDF
EF1753
Seq ID 444
MKERERVLELVKKGILTSEEALILLENMATEKDEKQIEKAAEKVDTQNTGTTNKEDQVADLMNALEKGESEGPTVDSFEEN
TQDSAEKDRENLERILDELATKANRASAELDEVNAEIAGIKEEIKEVAEEIGTLDTKEELDALTEDEQVQRKDLHVLLAQL
EEKLATQSTEKTALEEELKNIRKEQWKGQWNDTKEKVSSQFSEEWKDQATDTFNQVGGKVAEVGGQVGEFLKKTFNSFSDT
MNDNVEWKDIKMKVPGVATTKFEHEFNYPNPQASLIDVKVANGTVVFKTWDQEDVKVEAKIKLYGKMAGDSPMEAFLERSD
IDVDDETISFQVPNKRVKADLTFYLPKRTYDHVSVKLLNGNVLVEELTAKDVYTKSTNGTITFKKIDATMLEIEGVNGEIK
VLEGTILDNIIETVNGDVSISAAPESLSVSLINGDIRITAKEKTLRRVEASSANGNIKLALPNDLGVEGQVKTNLGSINSR
LTDIEVVREKKDRGNQQLHFRRVLEESMAQINASTTTGSIFLKDTDK
EF1791
Seq ID 445
MKKYLKITMVCILLVGFLAGCTNKNENKKKQKNTKEAVQLMSPSELTTLNTSVLLDFPDAIVQTAAFEGLYSLDEQDQLVP
AVAKALPMISEDGKTYTISLRKEAVWSNDDPVTAHDFEYAWKKMIDPKNGFVYSFLIVETIQNGAEISAGKLAPNELGVTA
VDDYTLKVTLKEPKPYFTSLLAFPTFFPQNQKVVEQFGADYGTASDKVVYNGPFVVKDWQQTKMDWQLAKNNRYWDHQNVR
SDIINYTVIKETSTALNLFEDGQIDVATLSGELAQQNKNNTLYHSYPTATMNYLRLNQKRKGQATPLANENLRKALALGID
KENLVNNIIADGSKALHGAITEGFVANPTTGLDFRQEAGNLMVYNKEKAQSYWKKAQAELGEKVNVELMVTDDGSYKKIGE
SLQGSLQELFPGLTIELTALPTEAALNFGRESDYDLFLIYWTPDYQDPISTLMTLYKGNDRNYQNPVYDKLLDEAATTYAL
EPEKRWATLIAAEKEVIETTAGMIPLSQNEQTVLQNDKVKGLNFHTFGAPLTLKNVYKEK
EF1800
Seq ID 446
MKHGKIKRFSTLTLLASATILVPLSTSAEETTNSSTETSSSMVEPTATEEKLWQSDFPGGKTGEWQDVIGKTNRELAGESL
AISRDAAAGNNAVSLNLDSPKLADGEVETKFKYTAGSGRTGVIIRGNTKDSWVFVGYNANGKWLVESPNSWNDSISGPTLN
EDTNYLLKVRYVGEKITIWLNTTLIYEGEPVLANGDKIPTEAGHVGVRLWYDKKIVNYDYFKNGPVDSIPEIVPEVTQIAP
VKVFTKIGVAPKLPKQVKVTYNTGKEANEAVRWNEIDPDAYKEPGTFEVDGTLENTNIKAKASIVVAKDNEAEKGDKISSA
DLTAVVDPQFPRIIRYEDPQSNQVIFNGQHEKIDQVMIDGKAYKATAEKQKSEANQAVYNVAVPEIGLRFTTTLTVSEGQE
LAMKLSDIREEGTKIHTISIPNQGLISVNSTDEGATFAGVVMNTGTNANNGNKNGDTIQDLTTTSQEETKKYMYGFLNTAN
YAASFWTNAYGDGSVDGSDNNRIHKQTKEAATGFVTTLSSGAWTYRPFDAPEDYTTGETPEVKVKFSKDSNDDNRVDWQDA
AIGFRSIMNNPMGAEKVPELVNQRIPFNFASQATNPFLVTLDESKRIYNLTDGLGQMNLLKGYQNEGHDSAHPDYGAIGQR
PGGEQALNQLIDEGHDLNAVFGVHINDTESYPEAKGFNEELVDPTKRGWDWLDPSYFIKQRPDTLSGRRYERFKELKQKAP
NLDYIYVDVWGNQGESGWASRQLSKEINSLGWFTTNEFPNALEYDSVWNHWSAEKDYGGTTTKGFNSTIVRFIRNHQKDTW
IISDNPLLGGAEFEAYEGWVGKTNFNTYRQKTFAINVPTKFLQHYQITNWETTTAADGQIYGTIKLANGAEKVTVTQADAN
SPRSITLNETEVLKGDAYLLPWNVNGQDKLYHWNPKGGTSTWSLDKKMQGKTNLHLYELTDQGRIDKGAIATTNNQVTIQA
EANTPYVIAEPDSIEPMTFGTGTPFKDPGFNEANTLKNNWKVFRGDGEVKKDANGDYVFSSEKERTEIKQDINLPKPGKYS
LYLNTETHDRKATVTVKIGGKKYTRTVNNSVAQNYIQADINHTSRKNPQYMQNMRIDFEIPDNAKKGSVTLAVDKGNSVTK
FDDLRIVERQTDIMNPDKQTVIKQDFEDTQAVGLYPFVKGSAGGVEDPRIHLSERNEPYTQYGWNGNLVSDVLEGNWSLKA
HKQGAGLMLQTIPQNIKFEPNKKYTVQFDYQTDGENVFTAGTINGELKNNNDFKPVGELTSTAADGQTKHYEAEIIGDASG
NTTFGIFTTGADKDFIMDNFTVTVESKK
EF1818
Seq ID 447
LMKGNKILYILGTGIFVGSSCLFSSLFVAAEEQVYSESEVSTVLSKLEKEAISEAAAEQYTVVDRKEDAWGMKHLKLEKQT
EGVTVDSDNVIIHLDRNGAVTSVTGNPVDQVVKIQSVDAIGEEGVKKIIASDNPETKDLVFLAIDKRVNNEGQLFYKVRVT
SSPTGDPVSLVYKVNATDGTIMEKQDLTEHVGSEVTLKNSFQVAFNVPVEKSNTGIALHGTDNTGVYHAVVDGKNNYSIIQ
APSLVALNQNAVKAYTHGKFVKTYYEDHFQRHSIDDRGMPILSVVDEQHPDAYDNAFWDGKAMRYGETSTPTGKTYASSLD
VVGHEMTHGVTEHTAGLEYLGQSGALNESYSDLMGYIISGASNPEIGADTQSVDRKTGIRNLQTPSKHGQPETMAQYDDRA
RYKGTPYYDQGGVHYNSGIINRIGYTIIQNLGIEKAQTIFYSSLVNYLTPKAQFSDARDAMLAAAKVQYGDEAASVVSAAF
NSAGIGAKEDIQVNQPSESVLVNE
EF1850
Seq ID 448
VQQNNNEISTQHQFDAYCKKVLRNEAKSIRKRNDKIKENEEPLNDLNEGKYSQTHLDEQDVYFLFGMEILISDQKLSAAID
QLSDTRKKIVLLYYFAGFNDTEIGKIFNMSTSGIWYQRTKAVEQLKMEYGLW
EF1877
Seq ID 449
MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVESGNLYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMY
GLYAITNFIWTVSLYLSNATGYVVQEAYKLDFISDTAESIGKNIQTLADITENGLQTSGFYFGFLLLMILALGVYVAYTGL
LKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVVPNSESQGKDSVDLIRDSLFSIQVQQ
PWLLLQFDDSNIEEIGEDRVNKILSVSPDENKGKDREEAVKAEIEDNDNANLSITKTMNRLGIVVFLVLFNIGISFFVFLL
TGIMLFSQILFIIFAMFLPISFLLSMLPTYESLGKMAIIRLFNTIMMRAGVTLVITTAFSISTMFFNISATYPFFMVAFLQ
IVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRRPQMLMNRKLRQLNRNVGRTLAFGGAAAVGNKLAKEQSKPKFKPAGS
SLRKNSRLPNDREVSSDSAKENPISNNKKQSRMNLTGRKIGKVLDTQALVKDKAKQVKDQVRNTPTNLKYILHKGLEKTKK
APKEFKRGLVQEKADREKLRDKQRQRRDEKMDEKRKNLGEVTDRHGKRRSNVSIKEDPKPQKDRILKNELPKRIVMVKPNS
EVRRRLVKQNLVARENNQLLSKKNSFQQVKQRSTLPKRANQKVQRIMKARPKPKSGDKK
EF2174
Seq ID 450
MKKIISGMLICTVLLNSFSVIASGEELVKTETTGETGLVTQATSETTTNSTEDTSSVTEENTSERDSSTSSTKEESLDSST
NSTTEEHSSVSETNTTDSKTEASQSSEVEKKTIDQDEADYQEAAREGTNHKKGTYAMKNGLSSRVARATVANVYANDPNLP
GKNFIDVSSWNGDISVAEYQKIKSYGVTGVSVKLTEGTWYVNPYAAGQIRNAKAAGLKVSAYHYSMYVSAATAQDEARYFA
QAAANSGLDKNTIMFNDAEDPTLTNNGRNAHANSVAFNQQLKALGYKNDALYVGKWWLTNGYIDTSAFGRDRVWVAQYPTT
PDSSMQWNNDHGAWQWSSQMYFPGLANYEGRPFDISMTYSNFLNMGNSSGPDLSKYYTTNPGRVIMKNDDTFYQDVAFRTP
GWRVKKNTLVTIKGIEYSSAGIPRLVTDQGYLTANKDYVLAAQSNIDLYFTTNPKKVRLKSDDYFYADPEFKQRLSKVSKG
TIVEVEDLAYTQSGIFRLKTAKGYLTANKNIVEQYKGLEDTYYTSNPGQVITRNEDTYYKDVEFKTKANDVSAGSVLKVTA
IEKTKSGIPRLKMANGYYFTANKNYVVATGSWIANYHTVNPGQIIMKNSDNFYGDSDFLYKGAAVSKGSLVPVVGIEYREN
QVPRLITQNGYLTANKSYAQKVVPNIKDYLYDYPEYVVMKTNDYYYQDVNFSKKGEFVSKDTLIKVLSVDYTENGLPRLKT
AKGYITANKSYVTKLVSNSDNYFTENPHQIIMRVSDKLYTDVEFRNGSRTLSSGTVVPVKGIEYSSKGVPRLKTEGGYLTA
NKNYVTAAGNTNNNYFITNPKKVKLLIDDCFYNNTEFTKKGQAVKKNTIVEVEAIEYTNNGIPRLKTKQGYLTANKWYVAK
VG
EF2224
Seq ID 451
MNKAVKNFVSYLMITMLFILNLLPMMNAFAQEVTSDAEKTVEKDGLKVIGKIEDTSSQEDIKTVTYEVTNTRDVPIKDLIL
KQKNTNDSPIKFVLDTLSEERGPTSLEEQAKVETNEKDQTTDIKLLNLQPNSTRKITINGQITTKASNKLLVSVLIEDNEK
GTLVIDLPSKDILADKESVSKEKQETSETKVENQANETASSTNEMTATTSNETKPEAGKAIESIQETALTQATESPEQPPL
KAQPTGPLVPPTPGRGFNTPIYQSVHKGELFSTGNTNLKIANENTAAAQTFLNTRGASSGYAINNFPLEFADVDNDPNTYN
SSRAYIDLNGAKEIAWAGLFWSASRYKGPAYGTNLSDEEISAPVQFTTPNGTVQRVSPQRYHRIDQDATNPGQRFGYNNTG
FSNYADVTSILQGDKSATGSYTLADIPMTSSLNGQYQYYNFSGWSLFVVTKDQASKSRAFSIYYGARGNAAGTNNEFTMSN
FLTAKQGNLDPIVTWFTVQGDKYWTGDNAQIKNSAGTWVNISNTLNPVNNAMNATVTDNDEHMVDKYPGKFAPDHPNFLDI
DIDRMAIPEGVLNAGQNQINFRTTSSGDDYSTNAIGFAVNAETPEFEIKKEIVEPKETYKVGETITYRVSLKNTKADSEAI
NSVSKDALDGRLNYLPGSLKIISGPNSGEKTDASGDDQAEYDETNKQIIVRVGNGATATQGGSYKADTAETIYEFKARINE
RAKANELVPNSATVEAVDILTSAKVNETSNIVEAKIADEQVTGKLTATKTVNNAKPKLGEEIEYTISFRNTIENGILNKVV
ITDQLPKGLTYVKDSLTSVGDEPKPTSLKETNGTITAEYPSITDMKERSIRFKVIVNEEAKAGETILNKAKVDDTVNPPEE
PEVPVVPETNAGKLAATKTVNNAKPKLGETIEYTISFRNTIENGVLNKVVITDQLPKGLTYVKDSLTSVGDEPKPTSLKEI
NGTITAEYPSITDTKERSIRFKVIVNEEAKAGETILNKAKVDDTVNPPEEPEVPVVPETNVGKLTATKTVNNAKPKLGEEI
EYTISFRNTIENGVLNKVVITDQLPKGLTYVKDSLTSVGDEPKPTSLKEINGTITAEYPSITDTKERSIRFKVIVNEEAKA
GETILNKAKVDDTVNPPEEPEVPVVPEAKEGKLTATKTVNNAKPKLGEAIEYTISFRNTIENGVLNKVVITDQLPKGLTYV
KDSLTSVGDEPKPTSLTEANGTITAEYPSITDMKERSIRFKVIVNEEAKAGETILNKAKVDDTVNPPEEPEVPVVPEAKEG
KLAATKTVNNAKPKLGETIEYTISRFNTIENGVLNKVVITDQLPKGLTYVKDSLTSVGDEPKPTSLKEANGTITAEYPSIT
DTKERSIRFKVIVNDEAKAGETILNKAKVGDGINPPEEPEVPITPEEPAKNKKETNKVVTDQNKPTKNSKNEIAINKKETS
KSSYLPKTGEKVQKIFAYLGVGLILIVLILYVIKRNKEKEE
EF2318
Seq ID 452
VSRERFQAREKKVQKMGRDGLVEQNRATGEEKRVSQRTADTAFDRARPTEREDIRRPAARGADTGKKRKQPRTAPEMAAEQ
TEPLLTPEYLADSTAEPPPDAPASMRGAVDMPLLDAPIAEEPTPAPVRKQPQKRGKKGKKKQSTKFTEDAARPGSEEKTAM
RGVEGAPAPKRRTSEGGSRLQFDKEEEPADPAADRAAIRKRQTAKLAEHAAKPEDKLKSDGNPKAGETRLKFEDTPKDTDI
SGDTAPDMPTRQQKKYNKAACRTERADRRVEQAQMKLPAKRRLHMDHQPDEASGKIKRRLRFEDEILPEYQKPSLSSRAGN
AAKTAAVLKLHGKLREYERDNVALESAHKIELVAEQGAGRVLRWERNRRRSKPYRTLRKAQQKAARAHTDLAWQTALRDNP
ELQRKNALAKWVQKQKIKRKYAQAAHEAKQSAKFTQNVLTATGKIARAIAQYAAAHKAVFLAVAMLALVVMFFATGLTSCT
AMLSGFQSSYISASYMANEQEICQSDLYYTEKETDLQIDIDKTEENYPGYDEYRYNIGEISHNPYELLGYLSTAFNAETFA
EVQPEIDRIFSRQYTLTREVIVETRYDDDGDPYDWYVLQTTLAVRPLSSVLQSSLTPGEQTDRYGVYMQTYGNRQAFGNPF
GFSWLGYVSSGYGWRVHPVNGEKSLHRGIDLAVAQGTPILAAQDGRVVSAGDAGSYGLCVVIEDDKGYQSRYAHCSSLNVS
AGQEVKRGDVIAAVGSTGNSTGPHLHLEVMLNGEYLNPYFFVDNGDDGTGAIPGTPGGPAIPDYSGEPMGDGSFEAMLREA
EKYLGYPYVWGGSHPSTSFDCSGYVSWVINQSGVGSVGRQTAQGLYNLCTPVSAVNAQPGDLIFFTGTYSSPGPVSHVGIY
VGGGRFIHCGDPISYANTGSPYWSAHLYGYGRIP
EF2704
Seq ID 453
MTNKEPWEAWSPAKVSSFQEDFLAWYEREKRNLPWRANTDAYRIWISEIMLQQTRVDTVIDYFYRFMEWFPTIQDLAEAPD
DKLLKAWEEGLGYYSRARNLKVAAQQIVSEGGKMPDTIEDIRSLKGIGPYTAGAIGSIAFNLREPAIDGNVMRVVSRLFEI
DADIAKASSRKVFEAAMLKIIDRERPGDFNQALMDLGSAVCTPTSPKCESCPLQQYCAAYQADKMTAYPVKSKKVKPKDVY
YVGTIIENKKQEFLLEQRPETGLLANMWLFPIEEISKKQFQQLQKLAQPAETEKQLTLELEPVTEPLVAEEPVNFFTDYET
VVWQKRTLGEVVHIFSHLKWHILVFYGRNTGELATLESQRWVAAQQFSDYVFPKPQQKMVELFKKEHEKK
EF2713
Seq ID 454
VEKSTVTSLTQETSATTINASTDSTALTAESEELPSLRQTLLNYVGMYGLTETLINRLSDDELDYAKKVSFHFVNQDISGT
ARMITKLYGEKPIPEDSYSTDYSTLTIDDLKNYLPQIRLSLIYVYDLNSDVVNNLSDQTLVDLINQVKVDYANQNYPSDVR
GDYGLAAMADKIKANDYTSINQSAESVSSDTTNTESTLQTTTSSSKKATTSSSTEHKKGIFPSTGEKKSVLFTIIGIILLS
LVSIFIIKNKKK
EF2802
Seq ID 455
MKKKILVGALVALFFMPLNVFAAKGDQGVDWAIYQGEQGRFGYAHDKFAIAQIGGYNASGIYEQYTYKTQVASAIAQGKRA
HTYIWYDTWGNMDIAKTTMDYFLPRIQTPKNSIVALDFEHGALASVPDGYGGYVSSDAEKAANTETILYGMRRIKQAGYTP
MYYSYKPFTLNHVNYQQIIKEFPNSLWIAAYPIDGVSPYPLYAYFPSMDGIGIWQFTSAYIAGGLDGNVDLTGITDSGYTD
TNKPETDTPATDAGEEIEKIPNSDVKVGDTVKVKFSVDAWATGEAIPDWVKGNSYKVQEATGSRVLLEGILSWISKGDIEL
LPDATVVPDKQPETTHIVQHGETLSSIAYQYGTDYQTLASLNGLANPNLIYPGQVLKVNGSATSNVYTVQLGDVLSSIAAK
LGTTYQTLAALNELANPNLIYPGQTLNY
EF2813
Seq ID 456
VSKQESDVVLNFKMNGEINYSRTIKDINKEMNLAATEYKNQVSAMDKNATQTEKLTATKKKLEKQLSLAEQRTKLLREEYE
KSVKETGEYSEQSQKLYKRLLESETGENKLRSALQSTNEALKEQGNLSIKTAEKLAKIEKAGDKIKSVGQKLSVGLTAPIM
GIGAASIAAFKELDECLDNITTATGATGSQLESLQASFKTVAGQIPADMQDISTGIGEVNTQFGLMDKQLEDTTGRMLKFS
EINGSDVSQSTINAKKSMDLFRLSIEDLPMILDSVSKTSQDTGVGVDQLFDAVNRGAPQLKAMGLGFSESTTLIGQMEKAG
IDSAGTLGYLAKASVVYAKDNDTMQEGLSGTIESIKGATTEQEKLTIASEVFGTKAASKMVEAIDSGALSMDGLADSAKNA
AGTVDQTFSDILDPIDQAKLAQNQFKIAMGELGEQVQIALLPAFQAATDAIKKVSEWFGSLTDSQKQTILKIAGVVAAIGP
VLVVLGTLASSISSLIPVIAFIASPIGLVIAAVAAWVAAIVVAYNKIGWFRDFINTSFKVIKDIVVGVFNVLKDTTKSTFD
FITGFIGGAMDGAAKIIGDYVNEIKRIFGGIVDFVTGVFTGDWSRAWQGVVDIFGGIFEGIAAVAKAPINAMITLINGFIG
GLNNIKIPKWVPGIGGKGFHIGKIPYLAEGGTILNGQAIVGEAGPELLTAKNGKTTVTPLSPEEKARGIGGALKGGNTIEQ
HVHIGQVDANNPSELDRMNRKLYKASAQAFYDLGGVPT
EF2820
Seq ID 457
MNKELLRQLQARHEKRLSDLQGKIESGEVREADLDSVNEEIDGLIDELKAIKAELGDDNSESGDGEGDDGTAKSKNTDDES
KEDREKDTNGNNDDKNEENRGGMISQEQRDGLLRTIHEGMEARNVMSNEQREKQIRKAFADFVIGNISESEARALGIETGN
GSVTVPEVIASEVISYAQEENLLRKYGTVIRTAGDVKYPILVKKAEANVNKKERTTDTTETAIQFDEILLDPAEFDALATV
TKKLLKMSGVPVEDIVVEELKKAYVRKEINYMFNGDDAGNENPGALAKKAVAFEKPVDLTAAGAGQKLYDALIEFKNTPVT
EVMKKGRFIINRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYFGDFSAFKIQEVIG
ALEIQKLVEKFSGKNQVGFQIYNLLDGQLVYSPFEPAVYRYEITKPVGG
EF3082
Seq ID 458
MKKKFLAMMAVSMMGLLMLSACQTNKKTADSATTETTAKTEVTVKDTNGQLTVPKNPKDVVVFDNGSLDTMDALGVGDRVV
GAPTKNIPAYLKKYQKVESAGGIKEPDLEKINQLKPDLIIISGRQQKYQEQLKAIAPTIYLAVDAKNPWASTKQNIETLGT
IFDKEEVAKEKITGLEKEIADVKKQAEASANNALVVLVNEGQLSAYGKGSRFGLIHDTFGFKAADDKIEASTHGQSVSYEY
VLEKNPGILFVVDRTKAIGGDDSKDNVAANELIQKTDAGKNDKVIMLQPDVWYLSGGGLESMHLMIEDVKKGLE
EF3256
Seq ID 459
MKVNKFVKGFAAIALSSLVLAACGADKKDNTTNSSSAASSETKKSTESSAPAKKVAGGDLKDGTYKLEEKNEKNGYRAVFE
MTVKDGKITESKYDNINADGKSKTEDTKYEESMKAKSGVGPKEYIKQLNDSFVKAQSASGVEVVTGATHSSESFQNYAQQL
IQAAQAGNTDTIEIDNGATLKDGTYSLKEKNDSNGYHTTFSMTVKDGKVTESNYDNVNADGKSKKDDTEYESKMKDVAGVG
PKEYIETLNKEFVKAMGEEDGSPAGVEVVTGATHSTHSFINYAQQLVNAAEKGDTTEIVVDNIVTK
EFA0021
Seq ID 460
MDFQNIQTVKELRYALKQYKQTLATTIFDDTQGYISVAFKKEVIEHDGKNVQFLCFYPNYEVALYPVETLLAYLEKLPEQA
IVTIKDHDGNYSSLFLTIDETIDQGNNYQWLVISDKNSYERFFFTAQESTQEQAFEKMLKELETDHDSTLEVVHNWLCRQT
DEALFLGILKEDRTLKGAMTYCIGKAQEQSKDQSSAMVADEVVFSWIKEYFLLEKLPETKAVGKVTTKKSETKPERKEVDE
QIDLFECV
EFA0044
Seq ID 461
MIILSGLFILGIGIVGGYQLATLPKLIEMKQHKAIQNHFNVKGNEYTYYQEDSENYILSLEDTEYRIKFSKNTPLKVVFTE
ILEPM
EFA0052
Seq ID 462
MKKIASTGLSILVATGVAGIGGNEVQAAEQAQPKTPENSSTEQPTVKATQTTEQAITEKQQQVTEKQAIVDQKQQVADTAK
KEKDAIDQSVKDQQAVVDQNKDALDQSQQAVTDQQAVVDEAKKVVDEATPSAIEKAKEQVATDTQAVDDQQKVVDQAQTDV
NQQQAVVEEKAKETNAAKVQNDKDQQAVTAAKQEQAKLEELAKNAEAEKAKAEKEQAAKEAELANKQKEEAKAKDQKTKDD
QAVADQQSVVTTSQEKVADAKADTAAKQADLTSKENALKDKQAATKQAQNTLDSSKEELKGHKGLKLPANFTPDYYKKLTE
QEKQAMEKEALALNKVFPENQADAAKVTEMINVKNPTEKQKQQMSDYVVGLINDVREKLGLQKLKISNQAMKFAWDVAKYD
NPKEFDHDVNAINRAAKKNGFKEFPGQNFYENLSMGRFTTQEGKVSMYDFEKAARNALVSMLMNDGHSGYSHLDSLLDANE
TNMAVSISGDLNDISAKIHIISYNQTKLVEANTYEEGTAPVFKSKETLQKEVATNQEKLATAQQAESDAQQARSASQQALN
TAKTTQATAEKELSVHKATLANLQAVATKSTTNYEEKVRQTAAAETNLQQTKDQLATINELIQNRAVVLEKAKTKVADAQA
IEQTSAKVLKEKQEAQKAEENTLNSLKEVLDLAKENLNQKQVALKTSTRSLARLENAQPTYEKAVNELNKAEAAVVQAKEA
YENSLKSLEELKEQQAVATLAYTQAQEDLSNAKLELQQYQGILRDLEAQQAEQRRQEALQEQVAKEQQRLEREAKQQQMLV
ASATSADKTPGLQQLSFSKQKEQPKAQTLTHSEPRKTKQVAKAQESLPHTGEQKSIWLTIFGLFMAVGAISFKNKRRKNS
EFC0004
Seq ID 463
VLLVFASCGVNKSTEDSKKTNETKVEQIATLSAGTPVQSLDPATAVDQTSVTLLANVMEGLYRLDEKNQPQPAIAAGQPKI
SNGGKTYTIVIRDGAKWADGTDITADDFVTAWQRVLDPKTASPNVELFAAIKNAKEISIGKQQKETLGVKSKGNKTIEIEL
EEPTPYFTDLLALTAYFPVQQNAVKEYGKEYGTTKENIVTNGAFTLTDLNGVGISDKWTIAKNPKYWDKKHVAMEKIKFQV
VKDINTGINLYNDGQLDDAPVAGEYSKQLENNKDFIRELSATTMFLEVNQRNKKSITSNKHARQAINFAIDREAISNKILT
NGSIPAKGVVPSKLVYNPKTGKDFTNSSLVFLDKSKAKDSWEKAKKELKGTDLSIDIMVNEEDLSKKLGEYLQNELQDTLD
GLKVSVTAVPATLQTERLNSGNFMIALSGWQADFADPVSFLANFESKSSLNHGGYANEEYDKLLKNNSSKRLQELKDAEKL
ILEDAGVIPLLQIGNAKLRNQKISEMKVHSIGAKYDYKTMEIK
EFC0012
Seq ID 464
MKKIILSSLFSAVLVFGGGSITAFADDLGPTDPATPPITEPTDSSEPTNPTEPVDPAEPPVIPTDPTEPSKPTEPTTPSEP
EKPTEPTTPIDPGTPVEPTEPSEPTEPSQPTEPTTPSEPEKPVTPEQPKEPTQPVIPEKPAEPETPKTPEQPTKPIDVVVT
PSGEIDKTNQSAGTQPSIPIETSNLAEVTHVPSETTPITTEAGEEIVAVDKGVPLTKTPEGLKPISSSYKVLPSGNVEVKA
SDGKMKVLPHTGEKFTLLFSVLGSFFVLISGFFFFKKNKKKA
EFC0021
Seq ID 465
MVESDRTWKEKVHVESQVEERLRKNLTLKSREATKLQKKALKTTKKEFKLSKKNYKQSLKKHKLTLKTGKEPLSINHLLDK
KKFVEQKKRKKAQKIAYKRTKKVDETRVVNQVKRETKEGLKREATQKVRTTLTQEDTLNEAMTLYEKTQQAKFNMRTALKT
GKTVKNLSVKTAKDTYGLGNRLFNFSRGRGFQRTPKDFTLKKQLMKQLRNRAMRFKAAKEAKKAEQGIGLIRSFFNGQKTL
GKVAALILKNPISWVVLLVLFLVFLLSGVASSTQKPAIVQEEEDLTASWTYFTKLDAQHTDDNNLFYSNIDDVLFYMNYRY
DDFKLLDMDSTGTKNFETILSELWTALNGKKPDYQLKTMQSLETDKKSSYFIEEEQAKHYQEIKKELGYQTLDDLLSFPVK
TDALIVNKRYGYDKSKEKLTLYKGIDVLIEDNQPFHSPMNGQIVSVPDTETLVIEKEKVARLTIRGVNTLRLTKGMDVEEG
TFLGNTKNSTVTFQYEKYKKETKDWFFVNPAFYFPRVTYTQTTLLGSAEFSPGASVEKRAQAVYDYLSKKGYTKEGISAIL
GNFSVESGINPKRAEGDYLNPPVGAHGNSWDEPSWLAMGGPQIYGGRFPNILHRGLGLGQWTDTADGGRPHTLLLnYAKGK
NKKWYDLQLQLDFIFDGDAPGSRTAADNVARSKVAATIPELTTYYLTVWEGNPGDKLGERIQAAQNWFTFFSRSGTPIGGS
GSEVFAQYKEKMQPLPTDRETKAGQGWPGNAYAPGNCTWYVFNRFAQLGKSIYPTMGNANQWVHNYSQTPGATLESAPKKG
DAVIFTNGVAGSSTQYGHVAYVEHVNSDGSFVISEMNVSGEYSMNWRVLKKEAGEYFMRLN
EFC0053
Seq ID 466
MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTEYIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVP
RTRDGHFSPTVFERYQRNEKALMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKNYP
YLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEYLKERGLQGTELVISDAHKGLVSAIR
KSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFREAVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQY
TVQGNSHNRLKSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK
ARFC0021.1
Seq ID 467
psekgnqgrvekrsdakstnhvnagryte
ARFC0021.2
Seq ID 468
enefklhkiglhsf1drvhqsvvlgvrslrsikkkcnhypqtekqkqdkagqamrmrqgivlgmclivlhs
ARF0324
Seq ID 469
lnkracesrsclrsrnprgkrnvqcdyckcrksac
ARFl627
Seq ID 470
vptlltnqlsvcqkhggqlfqiftkvghrllkqgptlpkllkkc
ARF1650
Seq ID 471
krlqkklkksisqpirtekvkqllgi
CRF0097
Seq ID 472
mallpnvtskpahgvaipvtgtfkpkkvttmpltipenkfva
CRF0257
Seq ID 473
scafchvsfvgkyfrspatgtttipafavirkfnaltrsarasgeekaisadntsstgnr
CRF0635
Seq ID 474
gsivdnspdinaigpaknktakiv
CRF1152
Seq ID 475
sqgqcygqlmllqyklfqnhkldhkkddlcanwstslkqlpcfp
CRFl720
Seq ID 476
sfvqssycsslrafsgkikfktipktvanaiednskvptlktkppmpmtkiteainkffflsklillsikifkplaaitpn
ktklipeitgpgilrikalnfptkpkatantaappitktlctlviastpifsp
Claims (37)
1.一种编码超免疫血清反应性抗原或其片段的分离的核酸分子,其包含选自如下的核酸序列:
a)与选自Seq ID No 1-2,4-8,10,12-18,20-23,25-26,29-43,45-62,64-74,76-77,79-83,85-89,91-92,94-114,117-126,128-146,148-170,373,375,379-381,387,392,394,397-399,407-408,410-411和415-424的核酸分子具有至少70%序列相同性的核酸分子,
b)互补于a)的核酸分子的核酸分子,
c)包含a)或b)的核酸分子至少15个连续碱基的核酸分子,
d)在严格杂交条件下退火于a)、b)或c)的核酸分子的核酸分子,
e)杂交于a)、b)、c)或d)中定义的核酸分子之核酸分子的简并性序列。
2.根据权利要求1的分离的核酸分子,其中序列相同性是至少80%,优选地至少95%,尤其是100%。
3.一种编码超免疫血清反应性抗原或其片段的分离的核酸分子,包含选自如下的核酸序列:
a)与选自Seq ID No 3,9,11,24,27,44,63,75,84,115-116,127,374,376-378,382-386,388-391,393,395-396,400-406,409和412-414的核酸分子具有至少96%,优选地至少98%,尤其是100%序列相同性的核酸分子,
b)互补于a)的核酸分子的核酸分子,
c)包含a)或b)的核酸分子至少15个连续碱基的核酸分子,
d)在严格杂交条件下退火于a)、b)或c)的核酸分子的核酸分子,
e)杂交于a)、b)、c)或d)中定义的核酸分子之核酸分子的简并性序列。
4.一种分离的核酸分子,包含选自如下的核酸序列
a)选自Seq ID No 90,147的核酸分子,
b)互补于a)的核酸的核酸分子,
c)杂交于a)、b)、c)或d)中定义的核酸分子之核酸分子的简并性序列。
5.根据权利要求1、2、3或4中任何一个的核酸分子,其中所述核酸是DNA。
6.根据权利要求1、2、3、4或5中任何一个的核酸分子,其中所述核酸是RNA。
7.根据权利要求1至5中任何一个的分离的核酸分子,其中从基因组DNA,尤其是粪肠球菌基因组DNA分离所述核酸分子。
8.一种包含根据权利要求1至7中任何一个的核酸分子的载体。
9.根据权利要求8的载体,其中所述载体经改造适于根据权利要求1至7中任何一个的核酸分子编码的超免疫血清反应性抗原或其片段的重组表达。
10.一种包含根据权利要求8或9的载体的宿主细胞。
11.包含根据权利要求1、2、5、6或7中任何一个的核酸分子编码的氨基酸序列的超免疫血清反应性抗原及其片段,其中所述氨基酸序列选自Seq ID No 171-172,174-178,180,182-188,190-193,195-196,199-213,215-232,234-244,246-247,249-253,255-259,261-262,264-284,287-296,298-316,318-340,425,427,431-433,439,444,446,449-451,459-460,462-463和467-476。
12.包含根据权利要求3、5、6或7中任何一个的核酸分子编码的氨基酸序列的超免疫血清反应性抗原及其片段,其中所述氨基酸序列选自Seq ID No 173,179,181,194,197,214,233,245,254,285-286,297,426,428-430,434-438,440-443,445,447-448,452-458,461和464-466。
13.包含根据权利要求4、5、6或7中任何一个的核酸分子编码的氨基酸序列的超免疫血清反应性抗原及其片段,其中所述氨基酸序列选自Seq ID No 260,317。
14.超免疫血清反应性抗原的片段,其选自包含表1a和表1c“推定的免疫原性氨基酸”和“鉴别的免疫原性区域的位置”栏的氨基酸序列的肽,表2的血清反应性表位,尤其是包含如下氨基酸的肽:4-10,14-21,30-36,59-68,77-82,87-93,96-105,112-121,125-133,135-141,150-162,164-183,192-203,207-213,215-226,228-234,241-247,250-285,302-308和135-148位的Seq ID No 171;15-57,60-73,77-101,108-134,136-177,185-201,203-217,226-240,244-254,272-277,283-288,292-343,354-370,380-398,406-437,439-453,473-490,532-538,584-590,595-601,606-612,664-677,679-704,715-724,731-753,759-772,786-794,814-862和657-684位的Seq ID No 172;4-9,15-36,41-47,54-60,75-81,114-120,131-146,152-158,174-182,194-202,208-215,218-226,255-271,276-285,290-295,302-311,318-328,330-344,352-359,365-377,388-395,398-405,426-432,439-449,455-500,505-513,531-537,542-552,554-561,587-595,606-612,718-734,763-771,775-782,792-801,805-812,822-828,830-843,849-863,876-894,905-911,919-926,935-947,949-958,968-979,1009-1016,1029-1045,1047-1056,1076-1081,1092-1106,1123-1133,1179-1200,1202-1211,1215-1223,1287-1299,1301-1306,398-431和1224-1237位的Seq ID No 173;17-47,74-80,90-97,126-133,137-148,167-173,179-185,214-223,250-255,270-283,329-338,342-350,352-358,360-367,372-383,398-404,411-421,426-432,435-446,452-462,472-479,515-521,582-592,611-618,623-629,642-659,666-673,678-689,704-725,732-737,744-757,768-789,824-834,842-849,862-868,877-887,904-916,923-928,941-947,962-974,982-992,1019-1030,1032-1044,1046-1052,1065-1075,1077-1087,1108-1121,1124-1132,1137-1151,1170-1182,1190-1206,1208-1214,1227-1233,1242-1251,1254-1273,1282-1298和792-825位的Seq ID No 174;19-31,39-67,82-91,104-110,113-128,149-155,161-181和137-155位的Seq ID No 175;6-18,54-63,69-85,110-127,142-156,158-167,169-211,238-246,248-257,276-311,339-349,371-380,385-391,394-403,421-438,451-456,483-489和449-468位的Seq ID No 176;5-15,24-34,50-56,61-83,98-121,123-136,149-162,166-194,202-215,221-227,229-332,337-360,367-402,404-415,427-433,444-462,471-478,487-498,511-518,521-544,550-563,568-574,580-587,597-607,610-616,624-629和468-498位的Seq ID No 177;11-19,32-49,57-63,65-71,80-89,91-133,166-181,183-191,201-230,234-257,264-291,297-303,305-314,316-335,337-354,359-366,368-374,383-388,394-405,408-442,446-470,483-490,499-505,513-538,544-555,557-563,568-590,598-608,617-623,627-636,641-647,667-685,687-693,710-723,733-739,742-754,769-815和366-388位的Seq ID No 178;4-16,30-35,42-53,67-76,82-87,101-108,112-130,132-138,147-152,161-183,187-208,218-225,265-281,295-303,305-317,322-334,338-357,360-368,370-383,387-394,400-419,421-430和255-336位的Seq ID No 179;19-27,36-47,59-66,76-83,101-112,118-125,142-147,162-180,185-196,225-240,246-263,286-304,314-319,327-333,353-367和194-214位的Seq ID No180;14-43,70-76,83-89,111-117,122-128,136-145,163-170,175-182,210-219,246-251,266-279,325-331,338-346,348-354,356-363,368-379,422-428,431-441,450-456,466-473,509-515,532-542,549-556,576-586,605-612,617-623,636-653,660-667,674-686,698-719,726-731,738-745,762-783,818-828,836-843,856-862,871-881,903-910,917-922,935-941,956-968,976-986,1013-1024,1026-1038,1059-1069,1071-1081,1102-1115,1118-1126,1131-1145,1164-1176,1187-1200,1202-1208,1221-1227,1236-1245,1248-1267,1273-1292,252-287和805-844位的Seq ID No 181;4-18,21-28,37-43,56-70,101-113,131-140,142-150,162-170,172-184,193-204,209-227,233-238,246-264和93-168位的SeqID No 182;14-20,44-50,61-70,77-96,99-106,129-142,168-181,187-196,205-221,225-241,277-296和257-281位的Seq ID No 183;18-29,43-54,64-76,78-84,88-103,125-149,159-176,198-218,230-242,256-271,279-285,287-293,300-306,325-331,344-351,357-364,371-397,400-414,419-464,485-515,517-526,529-537,548-553,573-580,584-590,603-620,639-661,676-681,687-700,716-761,772-780,785-790,795-803,823-836,848-853和106-134位的Seq ID No 184;7-13,19-42,44-51,55-75,87-97,99-110,112-118,129-135,141-156,158-178,213-220,230-286,294-308,323-338,345-352,355-365,370-392,394-419,437-446,454-460,474-497,515-526,528-546,569-575和128-141位的Seq ID No 185;12-20,24-33,45-70,73-84,86-94,103-116,118-124,135-142,163-170,176-200,202-224,226-234,237-248,250-262,265-287,296-307,334-341,347-356,361-369,382-396,405-415,418-427,431-439,443-449,452-461,467-474和113-146位的Seq ID No 186;13-38,44-50,52-59,66-72,83-94,103-110,116-124,131-137,158-180,199-204,218-233,241-264,269-317,326-342,350-356和70-86位的Seq ID No 187;29-35,49-59,63-84,86-97,103-111,113-126,130-144,150-158,174-198,221-231,250-264,266-273,291-298,310-318和70-90位的Seq ID No 188;19-25,28-52,60-66,71-76,131-142,149-155,157-178,181-213,218-223,237-242,250-257,260-266,272-279,282-290,321-330,373-385,393-407,441-453,461-475,509-521,529-542,577-589,597-610,643-655,663-677,703-718,729-734,358-464,495-570和604-685位的Seq ID No 189;4-29,51-76,116-136,158-173,179-193,207-215和86-111位的Seq ID No 190;5-23,45-70,79-90,93-107,114-122,142-151和18-36位的Seq ID No 191;9-51,68-120,133-149,158-180,186-206,211-220,222-237,248-293,296-310,317-339和248-260位的Seq ID No 192;14-24,44-63,69-98,108-119,123-136,155-161,164-176,180-193,203-208,215-223,239-247,274-281,283-289,296-304,306-313,315-327,331-341,343-353,357-386,392-405和205-246位的Seq ID No 193;5-13,16-23,36-42,53-63,70-83,96-102和14-34位的Seq ID No 194;4-13,19-35,49-56,59-76,83-107,121-134,144-153,157-164,166-186,194-202,209-216,231-253,257-264和98-134位的Seq ID No 195;16-32,38-47,58-68,78-89,98-114,117-123,132-141,146-156,164-170,179-188,196-212,219-230,232-237,244-263,265-274,278-293,297-303,306-326,339-349,352-359,362-367,373-379,384-394,396-406,423-443,451-461,465-484,490-497,504-511,523-533,537-547,550-556,558-566,573-579,586-593,598-609,615-642,647-665,671-686,693-713,723-728和332-378位的Seq ID No 196;6-21,34-44,58-64,66-74,79-87,114-127,129-143,154-162,174-189,205-214,241-262,266-273,278-297,319-324,328-338,342-351,390-398,409-415,422-435,458-464,471-477,481-486,506-531,534-540,542-550和315-389位的Seq ID No 197;4-28,39-45,52-58,69-82,93-115,122-128,135-140,146-163,177-192,209-215,221-232,271-284,331-337,341-352,360-378,383-390,392-401,409-422,428-435,462-470,474-480,482-496,531-539,541-549,551-560,562-569,576-582,598-618和98-127位的Seq ID No198;14-27,33-47,61-79,94-104,119-133和36-60位的Seq ID No 199;11-22,29-40,48-62,68-73,96-106,108-118,125-149和102-126位的SeqID No 200;4-11,45-55,76-83,86-102,105-112,138-144,147-153和20-48位的Seq ID No 201;12-20,28-56,62-68,72-82,93-99,101-107,120-133,135-145,178-186,208-232,279-292和36-64位的Seq ID No 202;6-14,23-48,65-82,92-134,140-181,188-219,228-238,244-253,255-261和124-145位的Seq ID No 203;11-25,31-38,53-59,62-71,89-99,125-133,151-157,182-190,195-203,208-215,219-229,249-262,267-275,287-295,298-316,318-325,328-334,344-353,357-363,371-377,385-391,396-415,425-436,438-457,471-485,538-552,554-561,606-625,630-636,646-653,669-679,695-704,706-715,722-747,763-773和714-738位的Seq ID No 204;10-29,33-45,50-60,70-79,83-95,118-124,136-157,176-184,192-205,207-216,223-234,240-246,258-268,275-283和37-56位的Seq ID No 205;4-24,27-38,46-54,66-72,81-97,112-119,128-137,152-157,173-179,185-214,219-225,227-248,262-284,286-295,301-307和117-134位的Seq ID No 206;26-43,49-56,60-71,74-82,87-98,110-116,131-146,154-164,169-178,183-189,205-214,241-246,255-268,275-292,305-314,316-323,326-340,346-363,397-402,419-429,440-446,452-461,467-475和29-66位的Seq ID No 207;7-16,21-39,48-58,61-78,82-89,109-136,138-150,152-176,182-247,255-261,267-332,336-345,347-358,362-368,371-392,394-404,407-472,490-498,505-513,527-544,554-582,603-611,614-620,632-638和500-523位的Seq ID No 208;24-46,77-83,90-97,99-118,123-166,168-177,204-212,229-239,248-262,273-282,287-293,300-319,321-337,340-352,357-366,391-402,411-428,442-450,464-471,479-489和19-40位的Seq ID No 209;9-23,25-34,53-58,70-86,90-97,99-116,118-128,131-141,185-191,228-233,237-253,255-261,264-271,273-280,302-312,319-349,351-359,362-369,376-383,387-394,398-406,419-434和20-31位的Seq ID No 210;15-22,37-43,71-87,105-115,121-127,135-142,152-158和32-52位的Seq ID No 211;6-12,18-29,37-47,50-58,65-83,85-91,94-99,108-123,142-150,156-163,183-193,215-222,242-249,252-258,261-270,285-308,318-326和1-95位的Seq ID No 212;9-61,65-133,144-155,166-173,175-221,233-276,278-313,329-368和210-233位的Seq ID No 213;11-29,33-39,46-51,65-93,107-113,134-143,147-154,166-177,181-188,214-220,233-243,263-269和112-128位的Seq ID No 214;8-46,110-134,155-167,174-183,188-201,210-230,253-258,267-282,289-299,312-319,322-327,330-337,365-381,389-402,405-411,419-425,439-447,465-472,489-512,525-532,540-554,577-589,591-599,605-614,616-624,633-649和503-529位的Seq ID No 215;34-49,64-70,90-118,124-131,141-152,159-165和112-128位的Seq ID No 216;5-15,26-45,55-72,80-85,93-100,121-133,142-148,154-167,198-205,209-215,241-254,260-265,271-279和244-270位的Seq ID No 217;4-36,38-54,67-83,122-153,159-178,205-212,232-242,244-253,259-268,281-288,298-309,324-331,334-370,372-381,389-401,403-429,441-450,456-462,465-471,473-479,483-504,508-518,537-543,553-565,578-584,592-609,619-625,658-667,669-679,712-719,722-729,737-744,746-752,758-765和180-226位的Seq ID No218;6-17,23-32,49-56,61-67,76-83,85-103,105-111,120-132,145-171,175-185,191-225,231-246和99-128位的Seq ID No 219;4-24,28-48,52-58,64-79,87-100,104-120,136-152,159-166和150-163位的Seq IDNo 220;15-27,65-71,77-99,104-121,128-154,183-216,223-229,234-255,277-287,296-308和77-97位的Seq ID No 221;8-18,44-76,102-109和49-57位的Seq ID No 222;5-14,28-40,42-51,54-60,77-83,89-100,117-124,146-172,176-204,216-231,237-244,267-278,324-334,342-348,396-401,427-433,438-450,452-457,465-471,473-481,491-500,509-515,523-544,550-556,558-569,589-595,606-618,625-632,640-649,665-671,678-688,691-698,717-723,728-734,781-789,800-805,812-821,833-868,873-879,889-905,929-939,988-998,1046-1061,1073-1079,1089-1096,1115-1124,1132-1140,1172-1196,1220-1226,1231-1249,1269-1277,1287-1301,1307-1330,1350-1361,1369-1378,1387-1412,1414-1420,1422-1439,1484-1491,1513-1529,1552-1561,1576-1583,1606-1613,1617-1640,1647-1654,1665-1679,1686-1698,1709-1727,1736-1743,1750-1757,1771-1790,1801-1807,1817-1823,1831-1842,1859-1868,1870-1882,1884-1891,1900-1906,1909-1914,1929-1935,1952-1960,1974-1988,2002-2011,2032-2063,2071-2081,2116-2124,2139-2147,2149-2159,2163-2190,2209-2215,2245-2253,2282-2287,2331-2342,2360-2370,2379-2393,2402-2408,2414-2421,2423-2430,2433-2439,2442-2450,2472-2478,2485-2493,2495-2503,2506-2512,2547-2554,2558-2564,2615-2625,2637-2652,2692-2698,2700-2706,2711-2723,2731-2740,2748-2753,2756-2762,2765-2772,2781-2798,2810-2824,2844-2852,2885-2899,2912-2922,2937-2944,2947-2970,2988-2998,3016-3025,3032-3037,3062-3071,3129-3148,3156-3161和530-607位的Seq ID No 223;31-36,57-62,79-85,90-96,99-112,120-146,162-185,193-203,208-217,219-226,239-253,283-290,298-304,306-321,340-349,351-361,365-372,386-395,407-438,473-486,537-551,558-568,576-594,598-604和75-95位的Seq ID No 224;14-19,24-30,34-42,45-52,54-64,66-82,95-105,107-118,126-163,171-177,184-201,210-215,260-269,3-279,288-304,321-327,358-364,370-375,380-387,394-404,407-413,421-431,436-451,465-474,504-511,531-552,578-587,614-626,629-636,638-671,691-715,719-729,733-745,752-759,768-777,785-792,794-802,805-824,844-854,867-880,885-891,893-902,907-924,939-948,955-964,966-975,987-1000,1012-1017,1023-1028,1050-1071,1083-1098,1102-1115,1133-1146,1170-1183,1204-1211,1213-1223,1262-1311,1313-1319,1346-1355,1366-1371,1383-1405,1409-1414和776-819位的Seq ID No 225;12-27,30-38,54-61,64-74,82-96,103-110,117-125,134-140,147-158,185-201,218-225,232-253,265-280,319-325,350-362,366-372,376-386,464-483,485-490,511-521,531-537,542-559,564-574,593-609,613-619,637-642,668-677和195-214位的Seq ID No 226;4-21,59-67,73-79,84-91,141-151,186-197,203-214,222-227,237-245,255-260,281-292,294-311,336-344,346-355,422-437,459-466,484-491和77-109位的Seq ID No 227;10-45,52-61,63-70,74-102,112-122,124-132,164-178,181-205,212-240,246-256和226-247位的Seq ID No228;38-50,53-63,78-87,89-111,126-152,169-176,179-186,193-228,254-267,275-282,288-304,309-318,325-341,346-353,358-367,384-395,404-427,429-435,456-465,467-501,510-521,523-536,541-548,552-560,563-584,589-595,597-620,625-637,639-645,661-666,712-729,734-741,743-750,775-806,809-816,818-840,842-850和693-714位的Seq ID No229;5-17,30-37,52-75,77-86,88-107,112-135,151-160,178-222,226-246,263-270,279-294,306-314,327-342,345-352,374-381,389-416,422-429,435-449,453-467,473-500,512-522,524-531,542-549,552-560,565-571,575-586,594-600,613-619,625-633,635-641,647-653,667-674,680-699,711-729,735-741,764-775,781-786,792-798,805-813,817-825,833-842,850-855,860-866,869-910,917-930,949-990和533-562位的Seq ID No 230;7-14,39-46,61-74,83-89,93-99,110-121,136-150,172-180,182-200,207-216,223-236,238-251,265-271,280-288,294-309,320-336,339-354,362-377,383-389,401-407,435-441,446-453,460-465,472-487,499-511,518-528,533-540,557-570,572-587,631-637,643-658,663-669,672-678,681-687,695-706,714-728和118-139位的Seq ID No231;5-19,24-30,56-64,69-79,93-100,102-111,117-123,125-133,174-182,185-199,205-224,268-275,311-336和102-125位的Seq ID No232;6-35,39-45,57-62,80-85,92-106,117-122,126-171,214-223,253-260,268-273,285-291,295-306,315-320,325-336,361-366和172-202位的Seq ID No 233;4-13,24-37,45-51,58-66,84-92,112-121,132-141,151-171,175-195,204-212,222-240,262-268,276-295,305-336,338-348,354-362和160-183位的Seq ID No 234;10-16,24-35,41-73,78-104,111-121,124-139,141-148,150-164,196-215,224-241,249-282,299-307,315-357,368-378,393-401和345-367位的Seq ID No 235;4-32,48-53,61-67,84-104,112-118和106-130位的Seq ID No 236;21-28,31-36,65-81,98-105,115-121,123-131,136-142,155-161,177-190和201-232位的Seq ID No 237;4-15,21-27,33-39,42-56,58-64,68-82,84-90,92-98,113-122,146-162,168-175,177-189,191-203,249-268,279-285,287-304,328-342,349-358,371-378,387-393,404-413,419-425,467-479,487-498,513-524,528-539,541-565,572-579,595-606,626-635,637-642和612-626位的Seq ID No 238;7-13,52-70,76-82,97-106,110-117和13-45位的Seq ID No 239;5-10,12-48,59-64,87-102,107-128,131-140,154-161,165-171,173-215和54-74位的Seq ID No 240;4-11,19-28,34-40,74-81,87-98,126-147,163-171,184-193,205-213和49-124位的Seq ID No 241;7-14,23-29,35-40,61-67,99-106,111-122,124-133,135-161,187-206,216-229,236-245,262-268,271-280和256-273位的Seq ID No 242;4-13,17-37,47-54,85-99,105-113,120-132,147-166,180-186,192-199,204-216和127-144位的Seq ID No 243;14-27,29-37,52-62,68-76,89-96,117-123,125-131,137-145,166-195,205-212,214-222,228-235,258-264,271-281,288-296,308-324,332-339,355-361,365-371和268-293位的Seq ID No 244;4-21,30-42,54-60,78-85,90-110,141-147,160-168,176-185,194-206,218-225,230-245,251-261,287-293,295-304,320-326,334-347,351-362,386-402,413-423,427-433,439-453,456-477,480-493,507-513,526-539,574-581,591-598,600-609,614-632,655-665,685-691,703-712,742-747,757-775,797-803,813-819,823-829,880-887,901-906,930-944,948-958,962-968,971-995,1002-1009,1017-1023,1036-1053,1069-1081,1107-1124,1129-1152,1178-1195,1211-1223,1249-1266,1271-1288,1334-1340,1346-1367,1-63和171-189位的Seq ID No 245;4-22,52-63,70-75,94-104,112-125,133-141,176-199,209-216,244-259,287-299,336-352,366-372,386-399,421-436,444-449,457-466,481-487,506-529,531-540和295-378位的Seq ID No246;9-30,43-49,58-75,86-96,119-131,138-147,162-167,181-201,208-214和16-121位的Seq ID No 247;4-27,52-58,80-90,92-100,108-114,118-143,169-176,189-198,247-261,281-287,307-317,323-329,352-363,372-381,396-411,413-426,429-440,442-450,456-461,468-479和1-73位的Seq ID No 248;4-32,47-52,57-63,71-78,92-104,126-142,153-175和145-163位的Seq ID No 249;17-23,35-41,51-70,73-86,104-125和105-129位的Seq ID No 250;25-32,41-50,75-85,87-103,115-122,138-149,164-171,188-210,212-220,224-234,256-273,288-299,304-310,330-336,357-365,382-390,399-405,414-421,440-446,454-461,480-486,502-514,518-540,543-553,561-567,572-580,582-588,595-630,633-651,672-681,691-709,760-767,813-832,841-848,852-866,873-893,919-925,927-933,940-955,957-978,984-997,1000-1010,1035-1040,1044-1051,1058-1064,1081-1091,1097-1124,1129-1138,1144-1150,1158-1165,1170-1180,909-936和1001-1031位的Seq ID No 251;4-12,19-26,31-41,49-64,66-86,101-117,119-127,134-142,152-161,163-172,179-188,209-218,234-241,276-291,294-300,307-320,324-341,346-356,373-387,389-397,410-416,418-436,444-454,460-472,481-486,500-507,511-535,541-549,553-559,579-586,602-607,613-620,628-640,654-663,671-678,681-691,709-722,741-754,766-774,778-786,797-803和212-226位的Seq ID No 252;4-10,15-27,34-54,60-73,79-88,101-115,120-136,154-162,167-172,222-240和126-195位的Seq ID No 253;5-16,18-25,29-35,57-63,86-91,107-121,123-131,170-179,185-199,204-226,250-255,262-274,291-296,325-347和1-38位的Seq ID No 254;7-19,22-34,36-42,48-54,60-66,71-76,104-110,118-133,135-145,158-164,167-174,182-193,196-204,217-229,251-290,293-299,309-315和288-318位的Seq ID No 255;43-51,55-61,66-73,80-90,103-127,133-142,174-180,185-196,203-210,229-235,239-251,258-266,272-278,289-314,316-326,340-346,355-361和14-27位的Seq ID No 256;4-25,27-33,35-41,52-74,76-89,99-124,138-144,146-159,167-182,184-191,193-206,211-223,232-240,249-257,270-279,281-287,293-310,322-341,347-356和292-322位的Seq ID No 257;5-13,28-38,43-60,67-72,98-116,122-134,137-151,167-174,177-195,197-216和99-195位的Seq ID No258;15-33,35-42,48-57,62-68,73-91,107-119,121-153,173-194,205-210,223-228,234-241,243-259,275-298,308-315,327-340,342-370,376-391,398-404,410-419和71-122位的Seq ID No 259;12-39,43-64,87-95,99-105,114-126,128-136,139-147,212-225和107-141位的Seq IDNo 260;6-33,40-45,60-75,79-86,121-129,131-137,161-167,172-178,186-195,203-212,236-244,257-264,278-294,306-312,345-358,368-381,386-395,404-410,412-418和198-270位的Seq ID No 261;18-31,34-41,50-56,69-83,99-106,129-141,147-153,159-168,170-178,190-198,200-212,221-232,237-255,261-266,274-292和118-216位的Seq ID No262;17-47,61-67,87-93,115-121,126-132,140-148,167-173,179-186,214-223,250-255,264-272,282-294,306-318,338-353,358-377,385-401,414-420,433-441,451-457,470-480,505-511,544-550,571-581,600-607,612-618,631-648,655-662,669-681,693-714,721-726,733-740,757-778,813-823,831-838,851-857,866-876,893-905,912-917,930-936,951-963,971-981,1008-1019,1021-1033,1035-1041,1054-1064,1066-1076,1097-1110,1113-1121,1126-1140,1159-1171,1182-1195,1197-1203,1216-1222,1231-1240,1243-1262,1268-1287和738-828位的Seq ID No263;19-28,40-46,51-57,68-74,81-87,98-108,111-121和20-36位的SeqID No 264;4-17,19-44,60-69,80-86,110-116和33-60位的Seq ID No265;8-16,18-28,42-50,53-75,79-86,94-99,122-128,136-142,149-163,166-173,198-212,254-272,288-295,304-318,324-329,343-348,351-364,367-383,389-395,411-420,427-436和11-56位的Seq ID No 266;19-25和6-24位的Seq ID No 267;6-39,59-68和44-63位的Seq ID No 268;5-14,21-28,38-53和29-41位的Seq ID No 269;4-13,31-41,56-65和32-56位的Seq ID No 270;5-12和4-21位的Seq ID No 271;4-18和17-32位的Seq ID No 272;4-10,23-33和14-30位的Seq ID No 273;26-34,44-53和35-52位的Seq ID No 274;1-19位的Seq ID No 275;4-17,23-30,32-37和6-23位的Seq ID No 276;5-33,40-58,61-66和45-66位的Seq IDNo 277;15-41,61-67和41-65位的Seq ID No 278;4-12,16-23,26-37和10-29位的Seq ID No 279;23-39和37-55位的Seq ID No 280;12-20和38-55位的Seq ID No 281;22-37和7-22位的Seq ID No 282;3-14位的Seq ID No 283;6-16,43-65,71-76和17-31位的Seq ID No 284;4-13,27-39,42-69和17-32位的Seq ID No 285;4-12,26-39和10-25位的SeqID No 286;2-31位的Seq ID No 287;6-38,49-62和39-55位的Seq IDNo 288;4-10,24-30和2-19位的Seq ID No 289;12-17,25-46和15-30位的Seq ID No 290;4-13和2-28位的Seq ID No 291;30-38和17-45位的Seq ID No 292;24-33,55-61和31-61位的Seq ID No 293;4-26,34-48和15-33位的Seq ID No 294;9-15和1-22位的Seq ID No 295;4-31和14-33位的Seq ID No 296;5-34,49-55,64-82和69-83位的Seq ID No 297;33-45和21-39位的Seq ID No 298;7-14,24-32,42-65,79-86和50-64位的Seq ID No 299;13-27,33-43,45-62和12-37位的Seq ID No 300;4-15,17-32和10-26位的Seq ID No 301;4-9,11-43,45-75和47-69位的Seq IDNo 302;4-18,22-37和17-34位的Seq ID No 303;4-14和5-24位的SeqID No 304;7-33,35-46和1-19位的Seq ID No 305;13-37,69-75和51-69位的Seq ID No 306;14-24,26-34,37-49,66-78和2-25位的Seq ID No307;17-46,52-57,59-64和54-68位的Seq ID No 308;4-22和13-25位的Seq ID No 309;8-40,53-63和29-50位的Seq ID No 310;16-28和32-40位的Seq ID No 311;14-20,22-28,39-45和2-22位的Seq ID No 312;4-13和12-31位的Seq ID No 313;15-21和2-17位的Seq ID No 314;4-17和20-36位的Seq ID No 315;4-19和9-18位的Seq ID No 316;4-14和3-19位的Seq ID No 317;4-21,32-40和21-39位的Seq ID No 318;4-13和10-27位的Seq ID No 319;18-31,39-47,75-87,89-98和79-99位的SeqID N0 320;15-21和9-24位的Seq ID No 321;4-14,18-27,30-53,55-64,68-74,81-98和22-40位的Seq ID No 322;7-24,44-51和35-60位的SeqID No 323;10-47和23-37位的Seq ID No 324;4-10,12-46和7-22位的Seq ID No 325;20-27和1-13位的Seq ID No 326;6-19,41-51和9-37位的Seq ID N0327;4-9,11-17和9-23位的Seq ID No 328;4-17,23-38,46-66,68-85和34-46位的Seq ID No 329;4-18,34-59,75-81和61-84位的Seq ID No 330;6-17和7-28位的Seq ID No 331;4-32,56-61和35-52位的Seq ID No 332;4-14,27-71,74-88,93-110,115-120,124-130,139-154,161-172和146-171位的Seq ID No 333;4-21和3-15位的Seq IDNo 334;12-17和9-26位的Seq ID No 335;10-21,45-58和51-67位的SeqID No 336;59-66,68-84和13-42位的Seq ID No 337;11-16和1-16位的Seq ID No 338;4-19,23-37和10-30位的Seq ID No 339;19-27,35-46,48-66,82-88,99-105,113-119和42-59位的Seq ID No 340;135-147位的Seq ID No 171;658-682位的Seq ID No 172;411-427和1226-1246位的Seq ID No 173;794-817和801-824位的Seq ID No 174;468-492和474-495位的Seq ID No 177;366-388位的Seq ID No 178;266-291,287-312和308-333位的Seq ID No 179;197-213和195-211位的Seq IDNo 180;252-275,262-285和812-830位的Seq ID No 181;94-112,97-120和104-128位的Seq ID No 182;257-281位的Seq ID No 183;106-134位的Seq ID No 184;70-86位的Seq ID No 187;358-383,378-402,397-421,499-524,520-545,541-566,622-646,641-665和660-684位的Seq ID No189;248-260位的Seq ID No 192;15-34位的Seq ID No 194;112-129位的Seq ID No 195;333-358和353-378位的Seq ID No 196;316-343,339-366和362-389位的Seq ID No 197;98-123和104-126位的Seq IDNo 198;20-43和23-48位的Seq ID No 201;124-145位的Seq ID No 203;717-738位的Seq ID No 204;37-56位的Seq ID No 205;118-134位的SeqID No 206;500-522位的Seq ID No 208;32-47位的Seq ID No 211;25-51,47-73和69-95位的Seq ID No 212;503-529位的Seq ID No 215;112-128位的Seq ID No 216;181-199位的Seq ID No 218;109-121位的Seq IDNo 219;150-163位的Seq ID No 220;77-97位的Seq ID No 221;564-586位的Seq ID No 223;75-94位的Seq ID No 224;776-798,784-808和794-815位的Seq ID No 225;196-212,78-100和85-107位的Seq ID No226;536-553位的Seq ID No 230;102-125位的Seq ID No 232;178-198位的Seq ID No 233;612-626位的Seq ID No 238;171-187位的Seq IDNo 245;296-320,315-339,334-358和353-377位的Seq ID No 246;47-71位的Seq ID No 247;1-25,20-45和40-64位的Seq ID No 248;146-161位的Seq ID No 249;910-935和1007-1030位的Seq ID No 251;212-226位的Seq ID No 252;126-152,148-173和169-195位的Seq ID No 253;288-310和293-316位的Seq ID No 255;293-312位的Seq ID No 257;154-170位的Seq ID No 258;72-95,90-112和97-121位的Seq ID No 259;135-150和146-163位的Seq ID No 262;799-827位的Seq ID No 263;23-43和33-53位的Seq ID No 266;44-62位的Seq ID No 268;6-22位的Seq ID No 276;37-54位的Seq ID No 280;40-54位的Seq ID No 281;7-21位的Seq ID No 282;4-11,16-34,48-55,67-77,87-106和153-183位的Seq ID No 425;22-40,49-65,70-91,95-109,111-125,146-207,209-216,219-225,229-244,251-270,274-286,292-309,316-329,335-355,358-370,376-388,392-419,425-430,435-441,448-455,464-478,486-515和437-465位的Seq ID No 426;5-19,25-31,43-48,60-79,88-100,105-129,148-171,187-193,243-263,316-322,334-340,345-351,369-378,381-391,399-404,474-483,502-517,525-530,558-568,579-596,622-627,631-638,644-651,653-660,674-680,687-693,721-728,743-753,760-775,788-795,806-813,821-828,835-842,847-859,868-887和300-347位的Seq ID No427;5-26,37-44,89-97,112-118,121-128,138-154,157-165,176-181,188-198,205-218,223-243,247-253,260-279和76-155位的Seq ID No428;4-29,41-46,49-68,82-88,121-147,158-164,187-193,195-208,229-236,244-249,251-263,269-275,307-313,337-343,348-381,392-398,402-408,432-438,85-117和194-239位的Seq ID No 429;5-12,14-22,28-34,40-46,70-79,84-129,152-165,174-182和37-109位的Seq ID No430;5-16,18-52,54-72,81-86,118-126,136-145,151-157,168-180,209-233,244-270,295-302,315-326,329-337,345-352,364-373,397-402,408-418,424-431,435-443,472-480,483-489,504-510,519-527,549-564,576-599,605-637,641-673和91-98位的Seq ID No 431;23-36,42-52,133-140,151-157,242-247,267-277,295-301,320-328,333-339,345-352,365-371,397-403,415-428,456-465,481-487,489-495,508-516,518-527,585-592,606-614,631-637,643-658,665-670,723-728,737-744,752-759,787-793,835-841,873-885,918-928,938-945,951-966,978-988,1015-1020,1030-1036,1044-1052,1058-1069,1071-1079,1081-1088,1113-1119,1125-1138,1141-1147,1164-1170,1172-1177,1190-1200,1214-1220,1230-1236,1239-1245,1262-1268,1270-1275,1288-1298,1312-1318,1328-1334,1337-1343,1360-1366,1368-1373,1386-1396,1410-1416,1426-1432,1435-1441,1458-1464,1466-1471,1484-1494,1508-1514,1524-1530,1533-1539,1556-1562和307-340位的Seq ID No432;19-25,35-41,44-50,66-72,74-79,92-102,116-122,132-138,141-147,164-170,172-177,190-200,214-220,230-236,239-245,262-268,270-275,288-298,312-318,328-334,337-343,360-366,368-373,386-396,410-416,426-432,435-441,458-464,466-478,504-524,79-148,177-246,275-344和373-442位的Seq ID No 433;7-14,16-23,33-39,46-53,72-79,92-115,123-130,156-175,179-187,214-220,239-246,266-274,302-325,338-354,360-370,375-390,392-401,421-428,430463和29-58位的Seq ID No 434;4-9,22-39,58-65,72-82,87-92,99-104,107-119,143-166,171-177,194-202,205-213,220-228,231-240,247-263,309-315,317-323,336-343和294-320位的Seq ID No 435;4-10,12-18,24-29,34-43,50-65,70-76,111-117,129-138,152-159,166-171,184-195,200-210,224-236,241-251,274-283,285-296,313-319,332-341,348-355,378-386,410-416,433-445,475-482,523-529,531-540,584-596,626-633,674-680,682-688,738-750,780-787,828-834,836-842,853-862,882-887,893-912和604-676位的Seq ID No 436;15-38,49-57,60-99,103-119,124-194,200-206,215-249,251-291,307-313,315-347,369-378,383-390,393-400,405-411,423-435,440-447,454-460,470-486,490-503,532-539,542-549,551-567,579-592和509-583位的Seq ID No 437;38-44,47-88,95-103,157-172,235-240,250-260,263-276,294-300,312-317,331-337,369-391,412-419,442-448,453-463,490-529,537-555,571-580,600-617,619-627,642-648,682-687,693-700,716-722,738-748,756-763,779-789,796-802,820-828,833-840,846-853,862-872,880-887,894-899,924-937,957-963,1006-1012,1043-1049,1063-1069,1076-1097和124-147位的Seq ID No 438;4-28,31-49,60-71,75-102,104-114,134-144,160-184,250-257,277-285,287-294,330-338,345-351,367-374,381-388,393-399,402-407,420-426,443-448,458-464,411-436和454-488位的Seq ID No 439;20-27,45-55,57-64,66-77,98-106,130-137,155-165,167-174,176-187,194-203,208-223,227-238,245-251,257-270,273-278,287-299,330-345,352-358,363-385,392-399,410-417,437-443,467-484,486-492,495-500,504-516,526-536和219-270位的Seq ID No 440;11-22,24-31,46-63,65-71,73-88,95-109,174-181,183-201,204-212,216-222,228-233,241-247和142-221位的Seq ID No 441;8-28,51-59,67-84,93-98,140-152,154-162,183-188和91-125位的Seq ID No 442;10-22,27-61和69-100位的Seq ID No 443;7-15,18-26,94-100,126-131,152-165,219-228,254-263,274-292,297-308,333-340,342-352,354-371,373-379,403-410,420-438,450-456,463-470,489-495,503-512和97-173位的Seq ID No 444;4-21,37-43,49-65,67-74,76-90,113-119,131-141,155-173,175-189,192-199,207-221,247-254,266-276,317-322,337-343,387-393,408-428,439-448,451-460,469-479,482-487,493-501,517-523,533-542和480-503位的Seq ID No 445;11-26,40-46,78-86,93-103,121-126,132-138,166-177,183-196,203-212,214-221,228-263,304-311,323-338,345-351,357-363,379-393,420-434,442-448,518-527,547-553,581-591,602-609,637-645,665-674,687-692,701-708,730-739,796-802,844-857,882-888,903-914,944-950,976-983,1027-1033,1049-1057,1066-1072,1085-1092,1120-1127,1137-1144,1153-1158,1165-1176,1181-1187,1221-1230,1238-1244,1269-1274和605-632位的Seq ID No 446;6-47,57-65,83-95,109-121,138-147,154-164,167-177,194-200,202-212,227-234,240-253,260-267,283-291,320-329,340-347,356-364,412-422,430-436,441-459,465-475,478-486,498-507和59-84位的Seq ID No 447;10-21,58-83,88-97,120-126和21-51位的Seq ID No 448;5-39,56-62,76-88,90-114,138-162,170-195,202-221,228-250,264-270,304-355,374-387,391-416,462-471,526-546,554-561,574-579,639-645,651-660,674-682,689-694和666-697位的Seq ID No 449;6-30,36-42,143-157,176-197,202-209,216-233,241-246,275-287,292-299,315-325,343-350,375-380,397-403,411-420,422-434,441-448,467-474,477-499,555-568,591-597,601-609,623-644,667-688,692-698,703-718,736-747,757-766,782-791,795-801,832-840,859-865和226-269位的Seq ID No 450;6-23,43-51,61-67,73-82,91-97,123-130,149-158,164-175,228-234,240-246,248-255,262-272,326-332,340-347,365-371,377-388,409-419,425-431,438-445,449-457,464-470,496-507,559-568,575-581,603-608,617-623,633-639,648-654,659-670,695-701,734-752,806-814,816-829,861-868,891-899,904-909,937-945,947-960,978-983,992-999,1022-1031,1068-1076,1078-1091,1109-1114,1123-1130,1153-1162,1199-1207,1209-1222,1254-1261,1284-1293,1330-1338,1340-1353,1371-1376,1385-1392,1415-1421,1433-1438,1460-1465,1470-1492和1422-1458位的Seq IDNo 451;82-94,111-118,125-131,206-212,261-266,310-320,328-338,345-351,353-360,414-420,424-434,440-447,451-500,506-516,548-561,566-572,584-591,601-622,630-636,650-659,661-667,674-699,703-711,717-729,736-744,752-759,765-771,813-822,826-842,852-868,870-877,881-895,897-906,913-922和602-671位的Seq ID No 452;12-18,20-25,43-54,56-65,73-79,82-88,99-111,136-142,153-169,171-183,195-223,229-248,255-260,272-277,281-292,298-319,322-329,332-351,363-379,381-389和275-304位的Seq ID No 453;4-9,34-48,65-77,101-106,111-131,138-153,186-191,230-250和148-219位的Seq ID No 454;4-23,30-35,42-53,67-76,82-87,101-108,112-130,132-138,147-152,161-183,187-208,218-225,265-283,295-303,306-317,322-334,338-357,360-368,370-383,387-398,400-419,421-430,104-182和240-304位的Seq ID No455;4-12,63-69,94-102,146-164,166-173,175-181,193-207,263-281,286-295,301-306,330-343,369-378,382-388,414-420,422-430,438-454,456-462,472-531,543-560,581-591,596-605,614-623,626-635,656-662,669-676,683-690,693-698,705-711,728-736,752-764和69-102位的SeqID No 456;6-12,43-53,141-147,164-179,185-195,197-206,227-235,237-271,288-305,308-317,335-341,351-357,365-376,386-395,397-416,422-447和11-35位的Seq ID No 457;16-24,50-65,73-84,88-99,114-124,130-146,181-187,193-203,214-220,236-247,250-258,287-297和50-113位的Seq ID No 458;4-25,50-55,76-82,117-123,131-137,139-148,157-166,239-245,253-258,266-275,277-292,300-306,51-83和93-161位的Seq ID No 459;6-22,34-43,51-86,93-100,110-116,150-161,164-171,180-187,197-218和168-237位的Seq ID No 460;4-27,55-60,74-82和10-46位的Seq ID No 461;6-19,25-31,43-49,60-79,88-100,105-129,148-161,164-171,187-193,243-263,316-322,334-340,369-378,381-391,398-404,460-466,474-483,502-509,511-517,525-530,558-567,579-596,622-627,631-638,641-651,653-659,674-680,687-693,710-716,720-727,743-753,760-775,788-795,806-813,821-828,836-842,847-860,865-880和258-377位的Seq ID No 462;4-11,25-64,71-79,88-94,107-120,123-132,167-188,231-237,240-246,261-267,306-311,330-342,351-358,389-395,406-418,429-434,439-448,483-501,511-520和71-143位的SeqID No 463;4-18,22-27,53-64,94-100,121-127,133-139,155-164,177-182,187-196,206-218,224-242,248-253,258-277和184-253位的Seq ID No 464;10-17,56-67,72-82,94-99,106-113,166-173,229-235,243-283,295-301,313-321,326-331,342-348,396-414,423-435,446-452,454-462,496-502,511-534,543-556,563-570,586-593,616-626,638-645,653-662,679-696,731-737,766-774,776-782,790-796,810-817,825-835,837-846和540-615位的Seq ID No 465;13-24,30-36,73-81,89-95,109-115,117-143,161-173,179-189,226-244,251-261,275-281,298-305,307-315,323-328,364-374,69-186和264-354位的Seq ID No 466;19-25和6-22位的Seq ID No 467;6-39,59-68和43-62位的Seq ID No 468;6-14,22-32和1-27位的Seq ID No 469;4-41和28-40位的Seq ID No 470;8-14和4-19位的Seq ID No 471;4-10,12-22,30-35和6-33位的Seq IDNo 472;4-16,24-33和37-54位的Seq ID No 473;2-23位的Seq ID No474;4-21,27-33,36-41和14-34位的Seq ID No 475;4-14,24-30,37-42,57-78,83-89,94-103,113-131和100-122位的Seq ID No 476。
15.一种生产根据权利要求11至14中任何一个的粪肠球菌超免疫血清反应性抗原或其片段的方法,包括表达根据权利要求1至7中任何一个的核酸分子。
16.一种生产表达根据权利要求11至14中任何一个的粪肠球菌超免疫血清反应性抗原或其片段的细胞的方法,包括用根据权利要求8或权利要求9的载体转化或转染合适的宿主细胞。
17.一种药物组合物,尤其是疫苗,包含如权利要求11至14中任何一个定义的超免疫血清反应性抗原或其片段或根据权利要求1至7中任何一个的核酸分子。
18.根据权利要求17的药物组合物,尤其是疫苗,特征在于它进一步包含一种免疫刺激物质,优选地选自尤其是聚阳离子肽的聚阳离子聚合物、免疫刺激脱氧核苷酸(ODN)、包含至少两个LysLeuLys基序的肽、尤其是人生长激素的神经活性化合物、明矾、弗氏完全或不完全佐剂或其组合。
19.根据权利要求1至7中任何一个的核酸分子或根据权利要求11至14中任何一个的超免疫血清反应性抗原或其片段用于制备药物制品,尤其是用于制备抗肠球菌感染的疫苗的用途。
20.一种抗体或至少其有效部分,所述抗体至少结合根据权利要求11至14中任何一个的超免疫血清反应性抗原或其片段的选择性部分。
21.根据权利要求20的抗体,其中所述抗体是单克隆抗体。
22.根据权利要求20或21的抗体,其中所述有效部分包含Fab片段。
23.根据权利要求20至22中任何一个的抗体,其中所述抗体是嵌合抗体。
24.根据权利要求20至23中任何一个的抗体,其中所述抗体是人源化抗体。
25.产生根据权利要求20至24中任何一个的抗体的杂交瘤细胞系。
26.一种产生根据权利要求20的抗体的方法,特征在于下面的步骤:
●通过给予非人的动物权利要求11至14中任何一个定义的超免疫血清反应性抗原或其片段而引发所述动物的免疫反应,
●从所述动物移出含有抗体的体液,以及
●通过对所述含有抗体的体液进行进一步的纯化步骤而产生所述抗体。
27.产生根据权利要求21的抗体的方法,特征在于下面步骤:
●通过给予非人的动物如权利要求12至15中任何一个定义的超免疫血清反应性抗原或其片段而引发所述动物的免疫反应,
●从所述动物移出脾或脾细胞,
●产生所述脾或脾细胞的杂交瘤细胞,
●挑选并克隆特异于所述超免疫血清反应性抗原或其片段的杂交瘤细胞,
●通过所述克隆的杂交瘤细胞的培养和可选的进一步的纯化步骤产生所述抗体。
28.根据权利要求20至24中任何一个的抗体用于制备治疗或预防肠球菌感染的药物的用途。
29.一种拮抗剂,其结合根据权利要求11至14中任何一个的超免疫血清反应性抗原或其片段。
30.一种鉴别能够结合根据权利要求11至14中任何一个的超免疫血清反应性抗原或其片段的拮抗剂的方法,包括:
a)在允许所述候选拮抗剂与所述超免疫血清反应性抗原或其片段结合的条件下,在能提供响应于所述候选拮抗剂与所述超免疫血清反应性抗原或其片段结合的可检测信号的组分的存在下,将所述分离的或固定化的根据权利要求11至14中任何一个的超免疫血清反应性抗原或其片段与所述候选拮抗剂接触;以及
b)检测响应于所述拮抗剂与超免疫血清反应性抗原或其片段的结合产生的信号的存在与否。
31.鉴别能够降低或抑制根据权利要求11至14中任何一个的超免疫血清反应性抗原或其片段与其相互作用伴侣之相互作用活性的拮抗剂的方法,包括:
a)提供根据权利要求11-14中任何一个的超免疫血清反应性抗原或其超免疫片段,
b)向尤其是根据权利要求20至24中任何一个的抗体的所述超免疫血清反应性抗原或其片段提供相互作用伴侣,
c)允许所述超免疫血清反应性抗原或其片段与所述相互作用伴侣相互作用以形成相互作用复合物,
d)提供候选拮抗剂,
e)允许候选拮抗剂和相互作用复合物之间发生竞争反应,
f)测定候选拮抗剂是否抑制或降低超免疫血清反应性抗原或其片段与相互作用伴侣的相互作用活性。
32.根据权利要求11至14中任何一个的超免疫血清反应性抗原或其片段的用途,用于所述超免疫血清反应性抗原或其片段的相互作用伴侣的分离和/或纯化和/或鉴别。
33.一种体外诊断与根据权利要求11至14中任何一个的超免疫血清反应性抗原或其片段的表达相关的疾病的方法,包括测定编码所述超免疫血清反应性抗原和片段的根据权利要求1-7中任何一个的核酸序列的存在,或根据权利要求11-14中任何一个的超免疫血清反应性抗原或其片段的存在。
34.一种细菌感染,尤其是肠球菌感染的体外诊断的方法,包括分析编码所述超免疫血清反应性抗原或其片段的根据权利要求1至7中任何一个的核酸序列的存在,或者根据权利要求11至14中任何一个的超免疫血清反应性抗原或其片段的存在。
35.根据权利要求11至14中任何一个的超免疫血清反应性抗原或其片段的用途,用于产生结合所述超免疫血清反应性抗原或其片段的肽,其中所述肽选自抗促成素。
36.根据权利要求11至14中任何一个的超免疫血清反应性抗原或其片段用于功能性核酸制造的用途,其中所述功能性核酸选自适体和镜面体。
37.根据权利要求11至14中任何一个的核酸分子用于制备功能性核糖核酸的用途,其中功能性核糖核酸选自核酶、反义核酸和siRNA。
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WO2004106367A3 (en) | 2005-06-16 |
EP2327720A1 (en) | 2011-06-01 |
AU2004242842B2 (en) | 2011-11-03 |
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AU2004242842A1 (en) | 2004-12-09 |
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