CN1711469A - 测量装置及荧光测量方法 - Google Patents
测量装置及荧光测量方法 Download PDFInfo
- Publication number
- CN1711469A CN1711469A CNA2003801033854A CN200380103385A CN1711469A CN 1711469 A CN1711469 A CN 1711469A CN A2003801033854 A CNA2003801033854 A CN A2003801033854A CN 200380103385 A CN200380103385 A CN 200380103385A CN 1711469 A CN1711469 A CN 1711469A
- Authority
- CN
- China
- Prior art keywords
- centerdot
- fluorescent dye
- light
- fluorescence
- sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 title claims description 14
- 238000012937 correction Methods 0.000 claims abstract description 55
- 239000007850 fluorescent dye Substances 0.000 claims description 98
- 238000005259 measurement Methods 0.000 claims description 57
- 239000000975 dye Substances 0.000 claims description 45
- 230000005284 excitation Effects 0.000 claims description 38
- 239000011159 matrix material Substances 0.000 claims description 23
- 238000006467 substitution reaction Methods 0.000 claims description 12
- 230000005855 radiation Effects 0.000 claims description 6
- 238000004364 calculation method Methods 0.000 abstract description 7
- 238000004040 coloring Methods 0.000 abstract 4
- KPHWPUGNDIVLNH-UHFFFAOYSA-M diclofenac sodium Chemical compound [Na+].[O-]C(=O)CC1=CC=CC=C1NC1=C(Cl)C=CC=C1Cl KPHWPUGNDIVLNH-UHFFFAOYSA-M 0.000 description 10
- 238000004458 analytical method Methods 0.000 description 9
- 239000000470 constituent Substances 0.000 description 9
- 238000006243 chemical reaction Methods 0.000 description 8
- 238000010586 diagram Methods 0.000 description 7
- 238000002835 absorbance Methods 0.000 description 6
- 239000000463 material Substances 0.000 description 5
- 239000000203 mixture Substances 0.000 description 5
- 238000003745 diagnosis Methods 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- 230000005540 biological transmission Effects 0.000 description 3
- 239000002131 composite material Substances 0.000 description 3
- 230000003287 optical effect Effects 0.000 description 3
- ABZLKHKQJHEPAX-UHFFFAOYSA-N tetramethylrhodamine Chemical compound C=12C=CC(N(C)C)=CC2=[O+]C2=CC(N(C)C)=CC=C2C=1C1=CC=CC=C1C([O-])=O ABZLKHKQJHEPAX-UHFFFAOYSA-N 0.000 description 3
- 238000002310 reflectometry Methods 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 238000013459 approach Methods 0.000 description 1
- 238000004590 computer program Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000007598 dipping method Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000003760 hair shine Effects 0.000 description 1
- 229910052736 halogen Inorganic materials 0.000 description 1
- 150000002367 halogens Chemical class 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 238000011017 operating method Methods 0.000 description 1
- 238000005424 photoluminescence Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000004065 semiconductor Substances 0.000 description 1
- 229910052724 xenon Inorganic materials 0.000 description 1
- FHNFHKCVQCLJFQ-UHFFFAOYSA-N xenon atom Chemical compound [Xe] FHNFHKCVQCLJFQ-UHFFFAOYSA-N 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
- G01N2021/6441—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N2021/6463—Optics
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N2021/6463—Optics
- G01N2021/6471—Special filters, filter wheel
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N2021/6482—Sample cells, cuvettes
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Optics & Photonics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating Or Analyzing Materials By The Use Of Ultrasonic Waves (AREA)
Abstract
Description
荧光染料的名称 | FAM | JOE | TAMRA | ROX |
激励峰值波长[nm] | 470 | 500 | 530 | 560 |
荧光峰值波长[nm] | 520 | 550 | 580 | 610 |
Claims (10)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2002330787A JP3903000B2 (ja) | 2002-11-14 | 2002-11-14 | 測定装置、蛍光測定装置及び蛍光測定方法 |
JP330787/2002 | 2002-11-14 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN1711469A true CN1711469A (zh) | 2005-12-21 |
CN100498296C CN100498296C (zh) | 2009-06-10 |
Family
ID=32310604
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CNB2003801033854A Expired - Lifetime CN100498296C (zh) | 2002-11-14 | 2003-11-13 | 测量装置及荧光测量方法 |
Country Status (7)
Country | Link |
---|---|
US (1) | US7256892B2 (zh) |
EP (1) | EP1562037B1 (zh) |
JP (1) | JP3903000B2 (zh) |
CN (1) | CN100498296C (zh) |
AT (1) | ATE541203T1 (zh) |
AU (1) | AU2003284547A1 (zh) |
WO (1) | WO2004044564A1 (zh) |
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102235976A (zh) * | 2010-04-28 | 2011-11-09 | 索尼公司 | 荧光强度校正方法、荧光强度计算方法及计算装置 |
CN102272584A (zh) * | 2009-01-22 | 2011-12-07 | 三井造船株式会社 | 荧光检测装置及荧光检测方法 |
CN102288566A (zh) * | 2011-07-29 | 2011-12-21 | 肖才斌 | 一种便携式多功能分析仪及其测试方法 |
CN102308198A (zh) * | 2009-03-11 | 2012-01-04 | 财团法人工业技术研究院 | 侦测与辨别分子目标物之装置与方法 |
CN109387492A (zh) * | 2017-08-10 | 2019-02-26 | 希森美康株式会社 | 试样分析装置及试样分析方法 |
CN114414542A (zh) * | 2021-12-20 | 2022-04-29 | 成都瀚辰光翼科技有限责任公司 | 用于基因检测的荧光信号检测方法、装置、介质及设备 |
Families Citing this family (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7426026B2 (en) | 2003-10-10 | 2008-09-16 | Hamamatsu Photonics K.K. | Method and system for measuring the concentrations of fluorescent dyes |
US7612871B2 (en) * | 2004-09-01 | 2009-11-03 | Honeywell International Inc | Frequency-multiplexed detection of multiple wavelength light for flow cytometry |
WO2007097171A1 (ja) * | 2006-02-23 | 2007-08-30 | Nikon Corporation | スペクトル画像処理方法、スペクトル画像処理プログラム、及びスペクトルイメージングシステム |
WO2007097170A1 (ja) | 2006-02-23 | 2007-08-30 | Nikon Corporation | スペクトル画像処理方法、コンピュータ実行可能なスペクトル画像処理プログラム、スペクトルイメージングシステム |
US7869888B2 (en) * | 2006-05-31 | 2011-01-11 | Tokyo Electron Limited | Information processing apparatus, semiconductor manufacturing system, information processing method, and storage medium |
JP4964568B2 (ja) * | 2006-11-24 | 2012-07-04 | 浜松ホトニクス株式会社 | 蛍光検出装置、蛍光検出方法および蛍光検出プログラム |
JP5011076B2 (ja) * | 2007-11-26 | 2012-08-29 | オリンパス株式会社 | レーザ顕微鏡 |
JP5234943B2 (ja) * | 2008-03-25 | 2013-07-10 | 一般財団法人電力中央研究所 | 光ファイバ複合架空地線の水検出方法および水検出装置 |
EP2304399A4 (en) * | 2008-07-07 | 2013-06-19 | Glaxosmithkline Llc | METHODS, APPARATUS AND SYSTEMS USING MULTIPLE ENERGY SOURCES FOR ANALYZING COMPOSITIONS |
WO2010079731A1 (ja) * | 2009-01-09 | 2010-07-15 | 三井造船株式会社 | 蛍光検出装置及び蛍光検出方法 |
WO2010143367A1 (ja) * | 2009-06-12 | 2010-12-16 | 三井造船株式会社 | 蛍光検出装置および蛍光検出方法 |
JP6075372B2 (ja) * | 2012-03-28 | 2017-02-08 | 横河電機株式会社 | 物質特性測定装置 |
JP2014016373A (ja) * | 2013-10-30 | 2014-01-30 | Fujifilm Corp | 塗布膜中の成分量の測定方法 |
EP3646006A1 (en) * | 2017-06-28 | 2020-05-06 | Ventana Medical Systems, Inc. | System level calibration |
Family Cites Families (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0175352B1 (de) * | 1984-09-19 | 1991-06-12 | Siemens-Elema AB | Verfahren und Anordnung zur schnellen Bestimmung der Parameter eines Probenmediums |
JP3207882B2 (ja) * | 1991-09-13 | 2001-09-10 | 株式会社日立製作所 | 分光蛍光光度計のスペクトル補正方法及びスペクトル補正機能付分光蛍光光度計 |
JPH0921749A (ja) | 1995-07-10 | 1997-01-21 | Olympus Optical Co Ltd | 多項目同時測定装置 |
US6015667A (en) | 1996-06-03 | 2000-01-18 | The Perkin-Emer Corporation | Multicomponent analysis method including the determination of a statistical confidence interval |
WO1998017992A2 (en) * | 1996-10-25 | 1998-04-30 | Applied Imaging, Inc. | Multifluor-fluorescence in situ hybridization (m-fish) imaging techniques using multiple multiband filters with image registration |
WO2000006991A2 (en) * | 1998-07-27 | 2000-02-10 | Ljl Biosystems, Inc. | Apparatus and methods for spectroscopic measurements |
US6704104B2 (en) * | 2000-03-16 | 2004-03-09 | Spectrumedix Llc | Multi-wavelength array reader for biological assay |
JP3690271B2 (ja) * | 2000-11-29 | 2005-08-31 | 株式会社島津製作所 | 核酸の塩基配列決定のためのマトリックス値を得る方法 |
JP2003083894A (ja) * | 2001-09-14 | 2003-03-19 | Sumitomo Electric Ind Ltd | 蛍光値補正方法、蛍光値補正装置、蛍光値補正プログラム及び前記蛍光値補正プログラムを記録した記録媒体 |
-
2002
- 2002-11-14 JP JP2002330787A patent/JP3903000B2/ja not_active Expired - Lifetime
-
2003
- 2003-11-13 AT AT03774006T patent/ATE541203T1/de active
- 2003-11-13 US US10/534,182 patent/US7256892B2/en not_active Expired - Lifetime
- 2003-11-13 AU AU2003284547A patent/AU2003284547A1/en not_active Abandoned
- 2003-11-13 CN CNB2003801033854A patent/CN100498296C/zh not_active Expired - Lifetime
- 2003-11-13 WO PCT/JP2003/014421 patent/WO2004044564A1/ja active Application Filing
- 2003-11-13 EP EP03774006A patent/EP1562037B1/en not_active Expired - Lifetime
Cited By (17)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102272584A (zh) * | 2009-01-22 | 2011-12-07 | 三井造船株式会社 | 荧光检测装置及荧光检测方法 |
CN102435591A (zh) * | 2009-03-11 | 2012-05-02 | 财团法人工业技术研究院 | 侦测与辨别分子目标物之装置与方法 |
CN102435591B (zh) * | 2009-03-11 | 2018-01-16 | 财团法人工业技术研究院 | 侦测与辨别分子目标物之装置与方法 |
CN102308198A (zh) * | 2009-03-11 | 2012-01-04 | 财团法人工业技术研究院 | 侦测与辨别分子目标物之装置与方法 |
CN102235976B (zh) * | 2010-04-28 | 2015-10-14 | 索尼公司 | 荧光强度校正方法、荧光强度计算方法及计算装置 |
US8825431B2 (en) | 2010-04-28 | 2014-09-02 | Sony Corporation | Fluorescence intensity correcting method, fluorescence intensity calculating method, and fluorescence intensity calculating apparatus |
US11340167B2 (en) | 2010-04-28 | 2022-05-24 | Sony Corporation | Fluorescence intensity correcting method, fluorescence intensity calculating method, and fluorescence intensity calculating apparatus |
CN102235976A (zh) * | 2010-04-28 | 2011-11-09 | 索尼公司 | 荧光强度校正方法、荧光强度计算方法及计算装置 |
US10295466B2 (en) | 2010-04-28 | 2019-05-21 | Sony Corporation | Fluorescence intensity correcting method, fluorescence intensity calculating method, and fluorescence intensity calculating apparatus |
US10656090B2 (en) | 2010-04-28 | 2020-05-19 | Sony Corporation | Fluorescence intensity correcting method, fluorescence intensity calculating method, and fluorescence intensity calculating apparatus |
US11262305B2 (en) | 2010-04-28 | 2022-03-01 | Sony Corporation | Fluorescence intensity correcting method, fluorescence intensity calculating method, and fluorescence intensity calculating apparatus |
US11994468B2 (en) | 2010-04-28 | 2024-05-28 | Sony Corporation | Fluorescence intensity correcting method, fluorescence intensity calculating method, and fluorescence intensity calculating apparatus |
CN102288566A (zh) * | 2011-07-29 | 2011-12-21 | 肖才斌 | 一种便携式多功能分析仪及其测试方法 |
CN109387492A (zh) * | 2017-08-10 | 2019-02-26 | 希森美康株式会社 | 试样分析装置及试样分析方法 |
CN109387492B (zh) * | 2017-08-10 | 2022-10-28 | 希森美康株式会社 | 试样分析装置及试样分析方法 |
CN114414542B (zh) * | 2021-12-20 | 2024-01-12 | 成都瀚辰光翼科技有限责任公司 | 用于基因检测的荧光信号检测方法、装置、介质及设备 |
CN114414542A (zh) * | 2021-12-20 | 2022-04-29 | 成都瀚辰光翼科技有限责任公司 | 用于基因检测的荧光信号检测方法、装置、介质及设备 |
Also Published As
Publication number | Publication date |
---|---|
JP3903000B2 (ja) | 2007-04-11 |
US7256892B2 (en) | 2007-08-14 |
WO2004044564A1 (ja) | 2004-05-27 |
ATE541203T1 (de) | 2012-01-15 |
EP1562037B1 (en) | 2012-01-11 |
US20060108540A1 (en) | 2006-05-25 |
EP1562037A1 (en) | 2005-08-10 |
AU2003284547A1 (en) | 2004-06-03 |
EP1562037A4 (en) | 2008-11-12 |
CN100498296C (zh) | 2009-06-10 |
JP2004163312A (ja) | 2004-06-10 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN1711469A (zh) | 测量装置及荧光测量方法 | |
CN1940511A (zh) | 光量测量设备和光量测量方法 | |
CN1146014A (zh) | 分光测量装置和自动分析装置 | |
EP1804051A1 (en) | Instrumentation and Method for Optical Measurement of Samples | |
CN1959366A (zh) | 采用窄光束标准光源的led光通量测试装置及测试方法 | |
EP1243925A3 (en) | Biochemical examination method | |
EP2635624B1 (en) | Optical sensor and sensing system for oxygen monitoring in fluids using molybdenum cluster phosphorescence | |
JP7412802B2 (ja) | 分光測定方法 | |
WO2004090517A1 (ja) | 蛍光寿命を利用した物質の定量用試薬、方法及び装置 | |
CN203443886U (zh) | 一种基于白光led的三色荧光检测仪 | |
CN107076609B (zh) | 使用光谱响应来归一化荧光仪器的响应 | |
CN104568799A (zh) | 以单色光合光扫描的光度吸收检测系统 | |
CN2831112Y (zh) | 集成有可变换光源的电泳分离与分析装置 | |
CN2844906Y (zh) | 测量化学元素浓度的色度读取器 | |
CN113189065B (zh) | 光学检测方法 | |
CN109374585B (zh) | 测量荧光量子产率的方法及装置 | |
Romani et al. | New portable instrument for combined reflectance, time-resolved and steady-state luminescence measurements on works of art | |
CN204462020U (zh) | 一种含外接激光光源的Hitachi F-4600荧光光谱仪 | |
CN103217526B (zh) | 光栅式荧光酶标分析仪测试标准板及其加工工艺 | |
CN2938079Y (zh) | 集成的光谱微传感器元件 | |
CN1328580C (zh) | 荧光光谱仪外设组合试样库发光性能的测试方法及装置 | |
CN1289919A (zh) | 自动波长校正的顺序电感耦合等离子体光谱仪及应用方法 | |
Bushma et al. | Optical sensor for the detection of mycotoxins | |
CN112782131A (zh) | 一种光谱检测系统和光谱检测方法 | |
CN212432983U (zh) | 一种荧光光谱检测系统 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
CI01 | Publication of corrected invention patent application |
Correction item: Inventor Correct: Nakashima Shinya False: Nakajima Shinya Number: 51 Page: 527 Volume: 21 |
|
CI02 | Correction of invention patent application |
Correction item: Inventor Correct: Nakashima Shinya False: Nakajima Shinya Number: 51 Page: The title page Volume: 21 |
|
COR | Change of bibliographic data |
Free format text: CORRECT: INVENTOR; FROM: NAKASHIMA NAOYA TO: NAKASHIMA SHINYA |
|
ERR | Gazette correction |
Free format text: CORRECT: INVENTOR; FROM: NAKASHIMA NAOYA TO: NAKASHIMA SHINYA |
|
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
CX01 | Expiry of patent term |
Granted publication date: 20090610 |
|
CX01 | Expiry of patent term |