CN1646917A - 鉴定与跨膜蛋白相互作用的化合物的方法 - Google Patents
鉴定与跨膜蛋白相互作用的化合物的方法 Download PDFInfo
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- G01N33/5035—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on sub-cellular localization
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- G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
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- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
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- G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
- G01N33/5058—Neurological cells
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- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
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- C07K2319/00—Fusion polypeptide
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- C07K2319/09—Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
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- G01N2500/00—Screening for compounds of potential therapeutic value
Abstract
Description
SCH-23390浓度 | 细胞百分比 | ||
细胞表面上的受体 | 细胞表面上和细胞核中的受体 | 细胞核中的受体 | |
10μM5μM1μM0.5μM0.2μM0.0μM | 58%46%39%36%32%0 | 32%42%46%44%49% | <10%12%15%20%19%62-70% |
(+)丁克吗浓度 | 细胞百分比 | ||
细胞表面上的受体 | 细胞表面上和细胞核中的受体 | 细胞核中的受体 | |
10μM5μM1μM0.5μM0.2μM0.0μM | 62%47%41%40%39%0 | 28%43%43%41%21% | 10%10%16%19%40%62-70% |
样品1 | 样品2 | 平均数 | NSB | SB | pmol/mg(皮摩尔每毫克)蛋白质 |
2739 | 3596 | 3167 | 1077 | 2090 | 1.42 |
样品1 | 样品2 | 平均数 | NSB | SB | pmol/mg(皮摩尔每毫克)蛋白质 |
16419 | 15362 | 15890 | 471 | 15419 | 13.15 |
Claims (122)
Applications Claiming Priority (10)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US37170402P | 2002-04-12 | 2002-04-12 | |
US60/371,704 | 2002-04-12 | ||
US37941902P | 2002-05-13 | 2002-05-13 | |
US60/379,419 | 2002-05-13 | ||
US38757002P | 2002-06-12 | 2002-06-12 | |
US60/387,570 | 2002-06-12 | ||
US42289102P | 2002-11-01 | 2002-11-01 | |
US60/422,891 | 2002-11-01 | ||
US44255603P | 2003-01-27 | 2003-01-27 | |
US60/442,556 | 2003-01-27 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN1646917A true CN1646917A (zh) | 2005-07-27 |
CN100399028C CN100399028C (zh) | 2008-07-02 |
Family
ID=29255685
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CNB038082977A Expired - Lifetime CN100399028C (zh) | 2002-04-12 | 2003-04-11 | 鉴定与跨膜蛋白相互作用的化合物的方法 |
Country Status (17)
Country | Link |
---|---|
US (8) | US7309576B2 (zh) |
EP (3) | EP1495330B1 (zh) |
JP (4) | JP4542342B2 (zh) |
CN (1) | CN100399028C (zh) |
AT (1) | ATE421697T1 (zh) |
AU (2) | AU2003218586B2 (zh) |
CA (1) | CA2521946C (zh) |
CY (1) | CY1108997T1 (zh) |
DE (1) | DE60325953D1 (zh) |
DK (1) | DK1495330T3 (zh) |
ES (2) | ES2321704T3 (zh) |
HK (2) | HK1128957A1 (zh) |
MX (1) | MXPA04011134A (zh) |
NZ (2) | NZ536063A (zh) |
PT (1) | PT1495330E (zh) |
SI (1) | SI1495330T1 (zh) |
WO (1) | WO2003087836A1 (zh) |
Cited By (2)
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CN102580091A (zh) * | 2011-01-12 | 2012-07-18 | 中国科学院上海生命科学研究院 | 增强阿片镇痛剂的镇痛作用的方法及试剂 |
CN108178790A (zh) * | 2018-01-18 | 2018-06-19 | 上海药明康德新药开发有限公司 | 一种将细胞膜分离以便对跨膜蛋白进行dna编码化合物库筛选的方法 |
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EP1495330B1 (en) * | 2002-04-12 | 2009-01-21 | O'Dowd, Brian, F. | Method of identifying transmembrane protein-interacting compounds |
EP2255832A3 (en) * | 2004-06-16 | 2011-02-09 | Affinergy, Inc. | IFBM's to promote attachment of target analytes |
CN1944647B (zh) * | 2006-08-23 | 2010-05-12 | 华东师范大学 | 多巴胺受体新亚型d5b基因及编码的蛋白与应用 |
JP4929462B2 (ja) | 2007-01-29 | 2012-05-09 | 国立大学法人高知大学 | 細胞膜上分子と相互作用する化合物の検出方法 |
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JP5230397B2 (ja) * | 2008-12-18 | 2013-07-10 | 独立行政法人科学技術振興機構 | 抗膜貫通型タンパク質抗体の抗原結合部位を決定する方法 |
US8643360B1 (en) * | 2012-09-02 | 2014-02-04 | George S. Cargill, III | In-water voltage gradient detector |
TWI472617B (zh) * | 2012-09-17 | 2015-02-11 | Nat Univ Chung Hsing | 調控大分子進入細胞內之蛋白質及其調控大分子進入細胞內之方法 |
US10513711B2 (en) | 2014-08-13 | 2019-12-24 | Dupont Us Holding, Llc | Genetic targeting in non-conventional yeast using an RNA-guided endonuclease |
EP3215611B1 (en) | 2014-11-06 | 2019-08-21 | E. I. du Pont de Nemours and Company | Peptide-mediated delivery of rna-guided endonuclease into cells |
BR112017024534A2 (pt) | 2015-05-15 | 2018-07-24 | Pioneer Hi Bred Int | sistemas de rna-guia/endonuclease cas9 inovadores |
EP3362560B1 (en) | 2015-10-12 | 2022-08-10 | DuPont US Holding, LLC | Protected dna templates for gene modification and increased homologous recombination in cells and methods of use |
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EP3387134B1 (en) | 2015-12-11 | 2020-10-14 | Danisco US Inc. | Methods and compositions for enhanced nuclease-mediated genome modification and reduced off-target site effects |
EP3708665A1 (en) | 2015-12-18 | 2020-09-16 | Danisco US Inc. | Methods and compositions for t-rna based guide rna expression |
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CA3022854A1 (en) | 2016-06-14 | 2017-12-21 | Pioneer Hi-Bred International, Inc. | Use of cpf1 endonuclease for plant genome modifications |
US20190100745A1 (en) | 2016-06-20 | 2019-04-04 | Pioneer Hi-Bred International, Inc. | Novel cas systems and methods of use |
BR112019007327A2 (pt) | 2016-10-13 | 2019-07-02 | Pioneer Hi Bred Int | método para obter uma célula vegetal, célula vegetal, planta, semente, polinucleotídeo-guia |
WO2018111765A1 (en) * | 2016-12-12 | 2018-06-21 | xCella Biosciences, Inc. | Methods and systems for screening using microcapillary arrays |
US11519009B2 (en) | 2017-01-09 | 2022-12-06 | University Of Massachusetts | Complexes for gene deletion and editing |
BR112020011011A2 (pt) | 2017-12-15 | 2020-11-17 | Danisco Us Inc. | variantes de cas9 e métodos de uso |
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JP2022526982A (ja) | 2019-04-05 | 2022-05-27 | ダニスコ・ユーエス・インク | 線状組換えDNAコンストラクトを使用してバチルス(Bacillus)のゲノムにドナーDNA配列を組み込むための方法及びその組成物 |
US20220162621A1 (en) | 2019-04-05 | 2022-05-26 | Danisco Us Inc | Methods For Polynucleotide Integration Into The Genome Of Bacillus Using Dual Circular Recombinant DNA Constructs And Compositions Thereof |
KR102329302B1 (ko) * | 2019-09-30 | 2021-11-22 | 한국과학기술연구원 | 도파민 d1형 수용체의 활성 측정용 적색 형광 단백질 기반 바이오센서 |
WO2023105244A1 (en) | 2021-12-10 | 2023-06-15 | Pig Improvement Company Uk Limited | Editing tmprss2/4 for disease resistance in livestock |
WO2024013514A2 (en) | 2022-07-15 | 2024-01-18 | Pig Improvement Company Uk Limited | Gene edited livestock animals having coronavirus resistance |
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US20030022277A1 (en) * | 1995-05-05 | 2003-01-30 | Daniel R. Soppet | Human neuropeptide receptor |
US6004808A (en) * | 1996-06-21 | 1999-12-21 | Aurora Biosciences Corporation | Promiscuous G-protein compositions and their use |
US5891646A (en) | 1997-06-05 | 1999-04-06 | Duke University | Methods of assaying receptor activity and constructs useful in such methods |
WO1999005177A1 (en) | 1997-07-28 | 1999-02-04 | The Regents Of The University Of California | Methods and compositions for identifying modulators of g-protein-coupled receptors |
KR20030032916A (ko) * | 2000-02-09 | 2003-04-26 | 휴먼 게놈 사이언시즈, 인크. | Ccr5에 대한 항체 |
WO2002014475A2 (en) | 2000-08-16 | 2002-02-21 | The Trustees Of Columbia University In The City Of New York | QUANTITATIVE ASSESSMENT OF erbB/HER RECEPTORS IN BIOLOGICAL FLUIDS |
EP1495330B1 (en) | 2002-04-12 | 2009-01-21 | O'Dowd, Brian, F. | Method of identifying transmembrane protein-interacting compounds |
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2005
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Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102580091A (zh) * | 2011-01-12 | 2012-07-18 | 中国科学院上海生命科学研究院 | 增强阿片镇痛剂的镇痛作用的方法及试剂 |
CN102580091B (zh) * | 2011-01-12 | 2014-12-10 | 中国科学院上海生命科学研究院 | 增强阿片镇痛剂的镇痛作用的方法及试剂 |
CN108178790A (zh) * | 2018-01-18 | 2018-06-19 | 上海药明康德新药开发有限公司 | 一种将细胞膜分离以便对跨膜蛋白进行dna编码化合物库筛选的方法 |
CN108178790B (zh) * | 2018-01-18 | 2021-09-07 | 上海药明康德新药开发有限公司 | 一种将细胞膜分离以便对跨膜蛋白进行dna编码化合物库筛选的方法 |
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