CN1633495A - 利用慢病毒载体产生基因修饰动物的方法和组合物 - Google Patents

利用慢病毒载体产生基因修饰动物的方法和组合物 Download PDF

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Publication number
CN1633495A
CN1633495A CNA028282469A CN02828246A CN1633495A CN 1633495 A CN1633495 A CN 1633495A CN A028282469 A CNA028282469 A CN A028282469A CN 02828246 A CN02828246 A CN 02828246A CN 1633495 A CN1633495 A CN 1633495A
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China
Prior art keywords
nucleotide sequence
virus
composition
lentiviral vectors
interested
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Pending
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CNA028282469A
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English (en)
Chinese (zh)
Inventor
R·拉马哈德兰
F·科恩特金
J·维基菲尔德
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Ozgene Pty Ltd
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Ozgene Pty Ltd
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Publication of CN1633495A publication Critical patent/CN1633495A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C12N15/867—Retroviral vectors
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/105—Murine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/15011—Lentivirus, not HIV, e.g. FIV, SIV
    • C12N2740/15041—Use of virus, viral particle or viral elements as a vector
    • C12N2740/15043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Environmental Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Animal Husbandry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Veterinary Medicine (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Virology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CNA028282469A 2001-12-21 2002-12-19 利用慢病毒载体产生基因修饰动物的方法和组合物 Pending CN1633495A (zh)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US34484101P 2001-12-21 2001-12-21
US60/344,841 2001-12-21
US39847602P 2002-07-25 2002-07-25
US60/398,476 2002-07-25

Publications (1)

Publication Number Publication Date
CN1633495A true CN1633495A (zh) 2005-06-29

Family

ID=26994121

Family Applications (1)

Application Number Title Priority Date Filing Date
CNA028282469A Pending CN1633495A (zh) 2001-12-21 2002-12-19 利用慢病毒载体产生基因修饰动物的方法和组合物

Country Status (8)

Country Link
US (1) US20030167500A1 (enExample)
EP (1) EP1465981A4 (enExample)
JP (1) JP2005538685A (enExample)
KR (1) KR20040089096A (enExample)
CN (1) CN1633495A (enExample)
AU (1) AU2002367024A1 (enExample)
CA (1) CA2470527A1 (enExample)
WO (1) WO2003059923A2 (enExample)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102016029A (zh) * 2008-01-29 2011-04-13 学校法人庆应义墪 将外源基因导入灵长类动物的早期胚胎的方法和包含该方法的生产转基因灵长类动物的方法
CN101717788B (zh) * 2009-11-10 2012-06-20 中国农业大学 慢病毒感染胚胎的方法
WO2017214937A1 (zh) * 2016-06-16 2017-12-21 毛侃琅 一种促进 app 基因表达的慢病毒表达载体及其应用

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103397019A (zh) 2003-11-21 2013-11-20 雷维维科公司 干扰rna在生产转基因动物中的用途
US7732180B2 (en) 2004-05-07 2010-06-08 University Of Pittsburgh Of The Commonwealth System Of Higher Education Porcine Forssman synthetase protein, cDNA, genomic organization, and regulatory region
EP2573185A3 (en) * 2005-02-16 2013-06-05 Lentigen Corporation Lentiviral vectors and their use
AU2006292827B2 (en) 2005-08-09 2013-02-14 Revivicor, Inc. Transgenic ungulates expressing CTLA4-IG and uses thereof
GB0702694D0 (en) * 2007-02-12 2007-03-21 Ark Therapeutics Ltd Vectors
EP2675901B1 (en) 2011-02-14 2018-05-30 Revivicor, Inc. Genetically modified pigs for xenotransplantation of vascularized xenografts and derivatives thereof
US10828330B2 (en) 2017-02-22 2020-11-10 IO Bioscience, Inc. Nucleic acid constructs comprising gene editing multi-sites and uses thereof
US12440578B2 (en) 2017-02-22 2025-10-14 Io Biosciences, Inc. Nucleic acid constructs comprising gene editing multi-sites and uses thereof
KR20230107335A (ko) 2020-11-20 2023-07-14 레비비코르 인코포레이션 이종이식을 위한 성장 호르몬 수용체 녹아웃을 갖는 다중-전이유전자 돼지
CN118434281A (zh) 2021-09-20 2024-08-02 雷维维科公司 用于异种移植的包含十个基因修饰的多转基因猪
WO2025158071A1 (en) * 2024-01-26 2025-07-31 Sartorius Albumedix Limited Retroviral preparations containing albumin for enhancing transduction efficiency, uses thereof for cell transduction

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5633076A (en) * 1989-12-01 1997-05-27 Pharming Bv Method of producing a transgenic bovine or transgenic bovine embryo
ATE408015T1 (de) * 1995-04-14 2008-09-15 Univ Alabama Res Found Transfer system basierend auf einem fusionsprotein und seine anwendungen.
AU4984697A (en) * 1996-10-11 1998-05-11 The Texas A & M University System Methods for the generation of primordial germ cells and transgenic animal species
US6080912A (en) * 1997-03-20 2000-06-27 Wisconsin Alumni Research Foundation Methods for creating transgenic animals
US6207455B1 (en) * 1997-05-01 2001-03-27 Lung-Ji Chang Lentiviral vectors
ATE426036T1 (de) * 1997-11-14 2009-04-15 Cedars Sinai Medical Center Transfektion und transfer nicht humaner mannlicher keimzellen zur generierung transgener nicht humaner saugetiere
US6555342B1 (en) * 1998-06-03 2003-04-29 Uab Research Foundation Fusion protein delivery system and uses thereof
US6313373B1 (en) * 1998-10-30 2001-11-06 Case Western Reserve University Tissue specific promoters and transgenic mouse for the screening of pharmaceuticals

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102016029A (zh) * 2008-01-29 2011-04-13 学校法人庆应义墪 将外源基因导入灵长类动物的早期胚胎的方法和包含该方法的生产转基因灵长类动物的方法
CN102016029B (zh) * 2008-01-29 2014-11-26 学校法人庆应义墪 将外源基因导入灵长类动物的早期胚胎的方法和包含该方法的生产转基因灵长类动物的方法
CN102016029B8 (zh) * 2008-01-29 2017-08-01 学校法人庆应义塾 将外源基因导入灵长类动物的早期胚胎的方法和包含该方法的生产转基因灵长类动物的方法
CN101717788B (zh) * 2009-11-10 2012-06-20 中国农业大学 慢病毒感染胚胎的方法
WO2017214937A1 (zh) * 2016-06-16 2017-12-21 毛侃琅 一种促进 app 基因表达的慢病毒表达载体及其应用

Also Published As

Publication number Publication date
JP2005538685A (ja) 2005-12-22
WO2003059923A3 (en) 2003-10-30
EP1465981A2 (en) 2004-10-13
KR20040089096A (ko) 2004-10-20
CA2470527A1 (en) 2003-07-24
AU2002367024A1 (en) 2003-07-30
WO2003059923A2 (en) 2003-07-24
EP1465981A4 (en) 2006-04-12
US20030167500A1 (en) 2003-09-04

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