CN1554741A - Stabilizing post-processing method for beneficial microbial preparation - Google Patents
Stabilizing post-processing method for beneficial microbial preparation Download PDFInfo
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- CN1554741A CN1554741A CNA2003101175113A CN200310117511A CN1554741A CN 1554741 A CN1554741 A CN 1554741A CN A2003101175113 A CNA2003101175113 A CN A2003101175113A CN 200310117511 A CN200310117511 A CN 200310117511A CN 1554741 A CN1554741 A CN 1554741A
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- thalline
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Abstract
The stabilizing post-treatment of beneficial microbial preparation includes mixing manufactured bacteria seed with the protecting agent comprising glycerin 15-20 wt%, glucose 8-10 wt%, peptone 10-15 wt%, yeast powder 5-10 wt% and soybean oligose 50-60 wt%; mixing with zeolite powder via stirring; drying in the air to produce bacterial powder; and mixing the bacterial powder with pre-mixture of bean residue 30-40 wt%, rice bran 40-55 wt% and yeast powder 10-20 wt%. The said stabilizing post-treatment can prolong the preservation period of the bacterial preparation, maintaining the high activity, and raise the stability of the product.
Description
Technical field
The present invention relates to a kind of aftertreatment technology of microbial preparation, relate in particular to a kind of microbial preparation stabilization aftertreatment technology.
Background technology
There is the low problem of stability in the product of (microbial preparation class) enterprise of numerous domestic fermentation at present, and the activity that product is often deposited 1 year its thalline just reduces greatly, and the viable bacteria content of product is less than 1 * 10
7CFU/g has influenced the quality of product greatly.For this reason, take measures to prolong product preservation period, improve the product viable bacteria content, to keep the high reactivity of thalline be very necessary.
Summary of the invention
The object of the present invention is to provide a kind of effective microorganism preparation stabilization aftertreatment technology, with preservation period, the content that improves the product viable bacteria that prolongs product, the high reactivity that keeps thalline.
Purpose of the present invention is achieved by the following technical programs:
A kind of effective microorganism preparation stabilization aftertreatment technology provided by the invention the steps include:
A. the high speed centrifugation 50-60min under 3000-5000rpm of the bacterial classification after will fermenting collects thalline;
B. be to mix at 1: 1 by weight with thalline and bacterial classification protective material, described bacterial classification protective material is by weight percentage: 15-20% glycerine, 8-10% glucose, 10-15% peptone, 5-10% yeast powder and 50-60% soybean oligosaccharide;
C. the zeolite powder that then the mixed thalline of step b is added again 2 times of quality of thalline mixes;
D. be 15-20 ℃ with thalline in temperature, dry and make the bacterium powder at the place, cool place of humidity 50-70%;
E. the Preblend that adds 15 times of quality in the bacterium powder of steps d preparation mixes, and described Preblend is by weight percentage: 30-40% dregs of beans, 40-55% rice bran and 10-20% yeast powder.
Bacterial classification described in the step a of the present invention is that Pseudomonas stutzeri belongs to bacterium (Pseudomonas stutzeri) 2 strains, Bacillus subtilus belongs to bacterium (Bacillus subtilis) 10 strains and genus lactubacillus bacterium (Lactobacillus) 1 strain.
The present invention has following beneficial effect: the present invention can prolong the high reactivity of the preservation period of product, the content that improves the product viable bacteria, maintenance thalline.Product after the fermentation is handled through the present invention, and the stability of product improves greatly, and product was preserved after 2 years, and its viable bacteria content can reach 50 * 10
8CFU/ml, the activity of thalline remains on more than 95%.
Embodiment
The embodiment of a kind of effective microorganism preparation stabilization aftertreatment technology of the present invention is as follows:
The used bacterial classification of present embodiment is that Pseudomonas stutzeri belongs to bacterium (Pseudomonas stutzeri) 2 strains, Bacillus subtilus belongs to bacterium (Bacillus subtilis) 10 strains and genus lactubacillus bacterium (Lactobacillus) 1 strain, the steps include:
A. with bacterial classification behind 1 ton of ferment tank, high speed centrifugation 60min under 5000rpm collects 50 kilograms of thalline;
B. per 100 gram thalline add 20 gram glycerine, glucose 10 grams, and peptone 10 grams, yeast powder 10 grams, soybean oligosaccharide (feeding level) 50 grams mix.
C. and then add feeding level zeolite powder 200 grams, mix;
D. with thalline 20 ℃ of temperature, dry and make the bacterium powder at the cool place of humidity 70% place;
E. in the bacterium powder of steps d preparation, add dregs of beans 2000 grams, rice bran 3000 grams, yeast powder 1000 grams mix.
Product after the fermentation is handled through the present invention, and the stability of product improves greatly, and product was preserved after 2 years, and its viable bacteria content can reach 50 * 10
8CFU/ml, the activity of thalline remains on more than 95%.The present invention sees Table 1 to the effect of effective microorganism preparation.
Table 1 the present invention is to the effect of effective microorganism preparation
| 0.5 year | 1 year | 1.5 year | 2 years | |
| Ordinary method viable bacteria content (CFU/ml) | ????1×10 8 | ????1×10 7 | ????5×10 6 | ????1×10 6 |
| The stabilization aftertreatment technology is handled viable bacteria content (CFU/ml) | ????5×10 9 | ????5×10 9 | ????5×10 9 | ????4×10 9 |
Claims (2)
1, a kind of effective microorganism preparation stabilization aftertreatment technology the steps include:
A. the high speed centrifugation 50-60min under 3000-5000rpm of the bacterial classification after will fermenting collects thalline;
B. be to mix at 1: 1 by weight with thalline and bacterial classification protective material, described bacterial classification protective material is by weight percentage: 15-20% glycerine, 8-10% glucose, 10-15% peptone, 5-10% yeast powder and 50-60% soybean oligosaccharide;
C. the zeolite powder that then the mixed thalline of step b is added again 2 times of quality of thalline mixes;
D. be 15-20 ℃ with thalline in temperature, dry and make the bacterium powder at the place, cool place of humidity 50-70%;
E. the Preblend that adds 15 times of quality in the bacterium powder of steps d preparation mixes, and described Preblend is by weight percentage: 30-40% dregs of beans, 40-55% rice bran and 10-20% yeast powder.
2, effective microorganism preparation stabilization aftertreatment technology according to claim 1, the bacterial classification described in its step a are that Pseudomonas stutzeri belongs to bacterium (Pseudomonas stutzeri) 2 strains, Bacillus subtilus belongs to bacterium (Bacillussubtilis) 10 strains and genus lactubacillus bacterium (Lactobacillus) 1 strain.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200310117511 CN1244680C (en) | 2003-12-24 | 2003-12-24 | Stabilizing post-processing method for beneficial microbial preparation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200310117511 CN1244680C (en) | 2003-12-24 | 2003-12-24 | Stabilizing post-processing method for beneficial microbial preparation |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1554741A true CN1554741A (en) | 2004-12-15 |
| CN1244680C CN1244680C (en) | 2006-03-08 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200310117511 Expired - Fee Related CN1244680C (en) | 2003-12-24 | 2003-12-24 | Stabilizing post-processing method for beneficial microbial preparation |
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| Country | Link |
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| CN (1) | CN1244680C (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101869138A (en) * | 2010-05-28 | 2010-10-27 | 江苏省农业科学院 | High-efficiency protection liquid for storage of microcapsule leaven |
-
2003
- 2003-12-24 CN CN 200310117511 patent/CN1244680C/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101869138A (en) * | 2010-05-28 | 2010-10-27 | 江苏省农业科学院 | High-efficiency protection liquid for storage of microcapsule leaven |
| CN101869138B (en) * | 2010-05-28 | 2012-05-30 | 江苏省农业科学院 | High-efficiency protection liquid for storage of microcapsule leaven |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1244680C (en) | 2006-03-08 |
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