CN112773752A - Whitening anti-aging raw material and preparation method and application thereof - Google Patents
Whitening anti-aging raw material and preparation method and application thereof Download PDFInfo
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- CN112773752A CN112773752A CN202110045293.5A CN202110045293A CN112773752A CN 112773752 A CN112773752 A CN 112773752A CN 202110045293 A CN202110045293 A CN 202110045293A CN 112773752 A CN112773752 A CN 112773752A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9794—Liliopsida [monocotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
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Abstract
The invention discloses a whitening anti-aging raw material and a preparation method and application thereof, and relates to the technical field of daily cosmetics. The preparation method of the whitening anti-aging raw material comprises the following steps: (1) pulverizing herba Dendrobii, and sterilizing; cooking rice for later use; (2) inoculating Aspergillus oryzae in rice to prepare rice koji; (3) mixing rice koji and herba Dendrobii, inoculating yeast and lactobacillus, and fermenting; (4) and filtering the fermentation liquor to obtain the whitening anti-aging raw material. The whitening anti-aging raw material prepared by the method has good whitening anti-aging effect.
Description
Technical Field
The invention relates to the technical field of daily cosmetics, in particular to a whitening anti-aging raw material and a preparation method and application thereof.
Background
As work becomes more busy and less time is spent in relaxation, people become more anxious about their age and color, which also leads to more frequent use of cosmetics. For cosmetics, in addition to the makeup effect, people pay more attention to the safety of cosmetics.
Fermentation is more and more emphasized by people in the production of cosmetic raw materials in recent years, particularly a plant fermentation technology, and the effects of improving the effect and reducing the toxic and side effects are realized by destroying the structure of plant fibers through fermentation, hydrolyzing macromolecular organic matters and releasing and decomposing more micromolecular functional substances. The role that fermentation plays in the production of raw materials is closely related to the type of microorganism and the growth status of the microorganism. Different kinds of microorganisms have different requirements on growth conditions and nutrient elements, and in order to ensure the normal growth of the microorganisms, the nutrients required by the microorganisms need to be additionally added into a culture medium, but the addition of the exogenous substances increases the difficulty of separating effective components after fermentation and increases the safety risk of raw materials.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provide a cosmetic raw material with good whitening and anti-aging effects and safety, and a preparation method and application thereof.
In order to achieve the purpose, the technical scheme adopted by the invention is as follows: a preparation method of a whitening anti-aging raw material comprises the following steps:
(1) treating raw materials: pulverizing dry strip of Dendrobium officinale, sieving, and sterilizing to obtain Dendrobium officinale powder; soaking rice in clear water for 30-60 min, cooking for 30-90 min, and cooling for later use;
(2) preparing yeast: inoculating aspergillus oryzae on the rice treated in the step (1), fully and uniformly mixing, and culturing for 3-6 days at a constant temperature of 30-40 ℃ to obtain rice koji;
(3) fermentation: adding the dendrobium officinale powder prepared in the step (1) into rice koji, adding water, and uniformly mixing to obtain a mixture A; then inoculating saccharomycetes and lactic acid bacteria, and culturing for 3-7 d at 20-30 ℃;
(4) and (3) filtering: and after the fermentation is finished, centrifuging the fermentation liquor, and filtering the supernatant to obtain the whitening and anti-aging raw material.
The main component of Dendrobium officinale Kimura et Migo is Dendrobium polysaccharide with average molecular weight of 4.23 × 105And usually the polysaccharide content is more than 40% by dry weight, so that the polysaccharide cannot provide sufficient nutrient elements for microorganisms such as yeast, lactic acid bacteria and the like. Therefore, additional nutrients need to be added in the fermentation process of the dendrobium officinale. However, the culture medium of yeast and lactobacillus needs to be added with a plurality of chemical reagentsSuch as magnesium sulfate, manganese sulfate, peptone, which are difficult to separate from functional components such as polyphenol, amino acid, etc. after fermentation. Therefore, the residue in the medium affects the safety of the raw material.
The invention uses a sectional fermentation process, firstly, aspergillus oryzae is inoculated into steamed rice, after fermentation for a period of time, dendrobium officinale powder and water are added into a bottle, and yeast and lactic acid bacteria are inoculated for the second stage of fermentation. In the invention, because Aspergillus oryzae has the capability of secreting amylase, protease and pectinase, rich nutrition of rice can be fully utilized to produce a large amount of reducing sugar, amino acid, vitamin and other components when the rice is fermented. The nutrient substances generated by decomposition can meet the growth requirements of yeast and lactic acid bacteria. Thereby improving the efficacy of the fermentation product of the dendrobium officinale. In the process, the introduction of the rice does not bring safety risk, and the components such as kojic acid generated by rice fermentation can improve the whitening and anti-aging functions of the fermentation product.
Preferably, in the step (1), the dendrobium officinale is crushed and then sieved by a 50-100-mesh sieve to obtain dendrobium officinale powder, and the particle size of the dendrobium officinale powder greatly affects the extraction efficiency of the dendrobium officinale.
Preferably, in the step (2), the mass ratio of the rice to the aspergillus oryzae is 50-200: 0.05-0.5.
Preferably, in the step (2), during cultivation, the aspergillus oryzae is wrapped by gauze, and air is exchanged every 12-24 hours, so that the oxygen environment required by the growth of the aspergillus oryzae is ensured.
Preferably, in the step (3), the mass ratio of the rice koji to the dendrobium officinale powder is 10: 1-3; the volume ratio of the mass of the rice koji to the mixture A is 1: 5-10 g/mL.
Preferably, in the step (3), the inoculation amount of the yeast is 1-5 wt% of the mixture A, and the inoculation amount of the lactic acid bacteria is 1-5 wt% of the mixture A.
Preferably, in the step (3), in the fermentation process, the culture medium is uniformly mixed by shaking every 12-18 hours and ventilation is performed once, so that the nutrient components and the strains can be fully mixed.
Preferably, in the step (4), the centrifugal speed is 3000-5000 rpm, and the centrifugal time is 10-20 min.
Meanwhile, the invention also discloses a whitening anti-aging raw material prepared by the method.
In addition, the invention also discloses a whitening anti-aging emulsion containing the whitening anti-aging raw material. The emulsion also contains EDTA-2Na, p-hydroxyacetophenone, propylene glycol, carbomer, xanthan gum, steareth-2, steareth-21, cetearyl alcohol, caprylic/capric triglyceride, NaOH, and 1,2 hexanediol.
Compared with the prior art, the invention has the beneficial effects that:
according to the invention, the dendrobium officinale is fermented by adopting a special process, so that the whitening and anti-aging raw material which is safe to human bodies and has good whitening and anti-aging effects is prepared. The preparation method is simple and suitable for industrial production.
Detailed Description
To better illustrate the objects, aspects and advantages of the present invention, the present invention will be further described with reference to specific examples.
Example 1
In an embodiment of the whitening and anti-aging raw material of the present invention, a preparation method of the whitening and anti-aging raw material of the present embodiment is as follows:
(1) treating raw materials: cutting dry dendrobium officinale strips into pieces, then crushing the cut dry dendrobium officinale strips by using an ultrafine crusher, sieving the crushed dry powder by using a 60-mesh sieve, and sterilizing to obtain dendrobium officinale powder; soaking rice in clear water for 30min, cleaning, steaming for 30min, and cooling;
(2) inoculating 0.05g of Aspergillus oryzae spore powder into 50g of cooled rice, mixing, culturing at constant temperature of 30 deg.C for 3d, wrapping with gauze, and ventilating once every 12h to obtain rice koji;
(3) fermentation: taking 50g of rice koji, adding 10g of dendrobium officinale powder, fixing the volume to 500mL by using sterile water, fully and uniformly mixing, and then inoculating 1 wt.% of saccharomycete and 1 wt.% of lactic acid bacteria; culturing at 20 deg.C for 3d, shaking and mixing culture medium uniformly every 12h, and ventilating;
(4) and (3) filtering: after fermentation is finished, centrifuging the fermentation liquor at 3000rpm for 20 min; and filtering the supernatant to obtain the whitening and anti-aging raw material.
Example 2
In an embodiment of the whitening and anti-aging raw material of the present invention, a preparation method of the whitening and anti-aging raw material of the present embodiment is as follows:
(1) treating raw materials: cutting dry dendrobium officinale strips into pieces, then crushing the cut dry dendrobium officinale strips by using an ultrafine crusher, sieving the crushed dry powder by using a 60-mesh sieve, and sterilizing to obtain dendrobium officinale powder; soaking rice in clear water for 60min, cleaning, steaming for 90min, and cooling;
(2) inoculating 200g of cooled rice with 0.5g of Aspergillus oryzae spore powder, mixing, culturing at constant temperature of 40 deg.C for 6 days, wrapping with gauze, and ventilating once every 24h to obtain rice koji;
(3) fermentation: adding 30g of dendrobium officinale powder into 200g of rice koji, fixing the volume to 1000mL by using sterile water, fully and uniformly mixing, and then inoculating 5 wt.% of saccharomycetes and 5 wt.% of lactic acid bacteria; culturing at 30 deg.C for 7d, shaking and mixing culture medium uniformly every 18h, and ventilating;
(4) and (3) filtering: after fermentation is finished, centrifuging the fermentation liquor at 4000rpm for 10 min; and filtering the supernatant to obtain the whitening and anti-aging raw material.
Example 3
In an embodiment of the whitening and anti-aging raw material of the present invention, a preparation method of the whitening and anti-aging raw material of the present embodiment is as follows:
(1) treating raw materials: cutting dry dendrobium officinale strips into pieces, then crushing the cut dry dendrobium officinale strips by using an ultrafine crusher, sieving the crushed dry powder by using a 60-mesh sieve, and sterilizing to obtain dendrobium officinale powder; soaking rice in clear water for 45min, cleaning, steaming for 60min, and cooling;
(2) inoculating 0.1g of Aspergillus oryzae spore powder to 100g of cooled rice, mixing, culturing at constant temperature of 35 deg.C for 5 days, wrapping with gauze, and ventilating once every 18h to obtain rice koji;
(3) fermentation: taking 100g of rice koji, adding 20g of dendrobium officinale powder, fixing the volume to 700mL by using sterile water, fully and uniformly mixing, and inoculating 2 wt.% of saccharomycetes and 2 wt.% of lactic acid bacteria; culturing at 28 deg.C for 5d, shaking and mixing culture medium uniformly every 15h during culture, and ventilating;
(4) and (3) filtering: after fermentation is finished, centrifuging the fermentation liquor at 4000rpm for 15 min; and filtering the supernatant to obtain the whitening and anti-aging raw material.
Comparative example 1
A whitening anti-aging raw material is prepared by the following steps:
(1) treating raw materials: cutting dry dendrobium officinale strips into pieces, then crushing the cut dry dendrobium officinale strips by using an ultrafine crusher, sieving the crushed dry powder by using a 60-mesh sieve, and sterilizing to obtain dendrobium officinale powder;
(2) fermentation: taking 10g of dendrobium officinale powder, fixing the volume to 400mL by using sterile water, fully and uniformly mixing, and then inoculating 2 wt.% of yeast and 2 wt.% of lactic acid bacteria; culturing at 28 deg.C for 5d, shaking and mixing culture medium uniformly every 15h during culture, and ventilating;
(3) and (3) filtering: after fermentation is finished, centrifuging the fermentation liquor at 4000rpm for 15 min; and filtering the supernatant to obtain the whitening and anti-aging raw material.
Application examples 1 to 3
The whitening anti-aging emulsion provided by the invention comprises the whitening anti-aging raw materials of the embodiments 1 to 3 respectively in application embodiments 1 to 3, and the components and the proportion are the same except the whitening anti-aging raw materials, and the specific formula is shown in table 1.
Application comparative example 1
The whitening anti-aging emulsion comprises the whitening anti-aging raw material in the comparative example 1 in the application comparative example 1, and the components and the proportion except the whitening anti-aging raw material are the same as those in the application examples 1-3.
TABLE 1 whitening anti-aging emulsion formula table (parts by weight)
Performance testing
(1) And (3) safety testing: 80 healthy volunteers with no history of skin allergy were selected for testing, and the volunteers were divided into 4 groups of 20 persons each, and tested in examples 1 to 3 and comparative example 1. The test method comprises the following steps: the inner sides of the left and right arms of the volunteers were used as a control test, 3 skin areas of 3 × 3cm were taken from the inner side of the left arm, 0.5g of the emulsion added with the corresponding raw material was applied each time, the inner side of the right arm was applied with the matrix emulsion not containing the raw material, and the skin reaction phenomena after 24h, 48h and 72h were recorded. The occurrence of redness, itching and eruption indicates that the product is unsafe.
Tests show that the volunteers applying the samples of examples 1-3 and comparative example 1 have no abnormal phenomenon after applying for 24h, 48h and 72h, which shows that the samples of examples 1-3 and comparative example 1 are safe and non-irritant to bodies.
(2) And (3) testing the whitening effect: skin color difference test probes and multifunctional skin test system CL400 and MPA9 were used to test skin whiteness values L and brightness values ITA °.
Testing the population: the normal population without the allergy history of the skin care product in the age of 18-55 years is random in sex, and the whiteness value of the inner side of the crank arm is 55-65. The left and right faces of the volunteers were randomly selected as the test areas, the test samples were used while the matrix samples were used, and the test samples were used once a day in the morning and at the evening for 4 weeks according to the test division. The number of test subjects per group was 10, and the results of the skin whiteness L and lightness ITA ° test of the test group and the substrate group before and after four weeks of use are shown in table 2.
Table 2 statistical results of skin whiteness values L and brightness values ITA ° before and after use
The average improvement effect of the application examples 1 to 3 is that L is increased by 2.18, and ITA is increased by 3.53; while the lifting effect of the matrix group was 1.12 increase in L and 2.49 increase in ITA °. Compared with the prior art, the emulsion added with the whitening and anti-aging raw material has better whitening effect and is obviously better than the application comparative example 1. Among 3 groups of experimenters, the whitening effect using the raw material prepared in example 3 was the best.
(3) And (3) anti-aging test: the polyphenol is a common antioxidant and anti-aging functional component in cosmetics, and the higher the polyphenol content is, the stronger the free radical scavenging capacity is, and the stronger the anti-aging capacity is when the cosmetic is applied to a human body.
Determining the content of total phenols by using gallic acid as a standard reagent and adopting a Folin phenol method; the scavenging ability of the above materials to 0.2mM DPPH radical was also measured, and the test was carried out by diluting the sample 10 times at the time of the test, and the test results are shown in Table 3.
TABLE 3 anti-aging test results
| DPPH radical scavenging ratio (%) | Total phenol content (mg/L) | |
| Application example 1 | 68.34 | 493.5 |
| Application example 2 | 72.62 | 532.1 |
| Application example 3 | 82.12 | 579.3 |
| Application comparative example 1 | 14.58 | 147.9 |
As can be seen from Table 3, the DPPH free radical clearance and the polyphenol content of the raw materials obtained in examples 1 to 3 are far higher than those of comparative example 1, which shows that the raw materials prepared by the method have obviously higher inoxidizability than the raw materials prepared by the method in the proportion, and the raw materials produced by the method have stronger antioxidant and anti-aging effects in practical application.
Finally, it should be noted that the above embodiments are only used for illustrating the technical solutions of the present invention and not for limiting the protection scope of the present invention, and although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions can be made on the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention.
Claims (9)
1. The preparation method of the whitening and anti-aging raw material is characterized by comprising the following steps:
(1) treating raw materials: pulverizing dry strip of Dendrobium officinale, sieving, and sterilizing to obtain Dendrobium officinale powder; soaking rice in clear water for 30-60 min, cooking for 30-90 min, and cooling for later use;
(2) preparing yeast: inoculating aspergillus oryzae on the rice treated in the step (1), fully and uniformly mixing, and culturing for 3-6 days at a constant temperature of 30-40 ℃ to obtain rice koji;
(3) fermentation: adding the dendrobium officinale powder prepared in the step (1) into rice koji, adding water, and uniformly mixing to obtain a mixture A; then inoculating saccharomycetes and lactic acid bacteria, and culturing for 3-7 d at 20-30 ℃;
(4) and (3) filtering: and after the fermentation is finished, centrifuging the fermentation liquor, and filtering the supernatant to obtain the whitening and anti-aging raw material.
2. The method for preparing the whitening and anti-aging raw material according to claim 1, wherein in the step (2), the mass ratio of the rice to the aspergillus oryzae is 50-200: 0.05-0.5.
3. The preparation method of the whitening and anti-aging raw material as claimed in claim 1, wherein in the step (2), during cultivation, the raw material is wrapped with gauze, and air is exchanged every 12-24 hours.
4. The preparation method of the whitening and anti-aging raw material as set forth in claim 1, wherein in the step (3), the mass ratio of the rice koji to the dendrobium officinale powder is 10: 1-3; the volume ratio of the mass of the rice koji to the mixture A is 1: 5-10 g/mL.
5. The method for preparing a whitening anti-aging material according to claim 1, wherein in the step (3), the inoculation amount of the yeast is 1 to 5 wt.% of the mixture A, and the inoculation amount of the lactic acid bacteria is 1 to 5 wt.% of the mixture A.
6. The preparation method of the whitening and anti-aging raw material as set forth in claim 1, wherein in the step (3), the culture medium is uniformly mixed by shaking every 12-18 hours and ventilation is performed once during the fermentation process.
7. The method for preparing the whitening and anti-aging raw material according to claim 1, wherein in the step (4), the centrifugal rotation speed is 3000-5000 rpm, and the centrifugal time is 10-20 min.
8. A whitening and anti-aging raw material prepared by the preparation method of any one of claims 1 to 7.
9. A whitening anti-aging emulsion comprising the whitening anti-aging material according to claim 8.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113813203A (en) * | 2021-09-01 | 2021-12-21 | 深圳浩涵生物科技有限公司 | Cherry enzymolysis fermentation product and preparation method and application thereof |
| CN114304335A (en) * | 2021-12-21 | 2022-04-12 | 昆明生物制造研究院有限公司 | Method for enriching active ingredients of dendrobium leaves through fermentation and application of method |
| CN118178296A (en) * | 2024-03-27 | 2024-06-14 | 植物医生(广东)生物科技有限公司 | Preparation method and application of plant composition for tightening and resisting wrinkles |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109691670A (en) * | 2019-01-25 | 2019-04-30 | 华南农业大学 | A kind of preparation method and applications of dendrobium candidum enzyme liquid |
| KR20190094642A (en) * | 2018-02-05 | 2019-08-14 | 주식회사 라비오 | A cosmetic composition comprising fermented dendrobium candidum and method for preparing the same |
| CN111135136A (en) * | 2020-03-26 | 2020-05-12 | 广州七遇生物科技有限公司 | Anti-aging skin care composition and preparation method thereof |
-
2021
- 2021-01-13 CN CN202110045293.5A patent/CN112773752A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20190094642A (en) * | 2018-02-05 | 2019-08-14 | 주식회사 라비오 | A cosmetic composition comprising fermented dendrobium candidum and method for preparing the same |
| CN109691670A (en) * | 2019-01-25 | 2019-04-30 | 华南农业大学 | A kind of preparation method and applications of dendrobium candidum enzyme liquid |
| CN111135136A (en) * | 2020-03-26 | 2020-05-12 | 广州七遇生物科技有限公司 | Anti-aging skin care composition and preparation method thereof |
Non-Patent Citations (4)
| Title |
|---|
| 胡文浪编著: "《黄酒工艺学》", 31 August 1998, 中国轻工业出版社 * |
| 舒旭晨等: "混菌发酵石斛酵素及其抗氧化活性研究", 《徐州工程学院学报(自然科学版)》 * |
| 蔡宛如主编: "《药食同源》", 31 August 2019, 浙江科学技术出版社 * |
| 陈周潭等: "米曲霉大米发酵提取物抗氧化活性的比较研究", 《现代食品科技》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113813203A (en) * | 2021-09-01 | 2021-12-21 | 深圳浩涵生物科技有限公司 | Cherry enzymolysis fermentation product and preparation method and application thereof |
| CN113813203B (en) * | 2021-09-01 | 2023-10-24 | 深圳丽颖生物科技有限公司 | Cherry blossom enzymolysis fermentation product and preparation method and application thereof |
| CN114304335A (en) * | 2021-12-21 | 2022-04-12 | 昆明生物制造研究院有限公司 | Method for enriching active ingredients of dendrobium leaves through fermentation and application of method |
| CN114304335B (en) * | 2021-12-21 | 2024-02-20 | 昆明生物制造研究院有限公司 | Method for fermenting and enriching active ingredients of dendrobium leaves and application of method |
| CN118178296A (en) * | 2024-03-27 | 2024-06-14 | 植物医生(广东)生物科技有限公司 | Preparation method and application of plant composition for tightening and resisting wrinkles |
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Application publication date: 20210511 |
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| RJ01 | Rejection of invention patent application after publication |