CN101869138A - High-efficiency protection liquid for storage of microcapsule leaven - Google Patents
High-efficiency protection liquid for storage of microcapsule leaven Download PDFInfo
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- CN101869138A CN101869138A CN 201010185715 CN201010185715A CN101869138A CN 101869138 A CN101869138 A CN 101869138A CN 201010185715 CN201010185715 CN 201010185715 CN 201010185715 A CN201010185715 A CN 201010185715A CN 101869138 A CN101869138 A CN 101869138A
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Abstract
The invention relates to a formula of high-efficiency protection liquid for the storage of a microcapsule leaven, which belongs to the technical field of biological engineering. The protection liquid is prepared from the following components in the optimum formula ratio: 0.9% of sodium chloride, 3% of glucose, 5% of cane sugar, 10% of glycerol, 0.2% of calcium carbonate and the like. The invention can solve the storage problem of the microcapsule leaven and effectively prolongs the service life of the leaven.
Description
One, technical field
The present invention is a kind of high-efficiency protection liquid for storage of microcapsule leaven prescription, belongs to technical field of bioengineering.
Two, background technology
Along with the fast development of China's fermented food industry, the leavening industry has been proposed new requirement, demand rises year after year.At present the most ofs large-scale Dairy Processing of China enterprise adopts the direct putting type ferment agent for sour milk of external import, its steady quality, produces easily capable and cell density can reach 10
11Cfu/g, however cost an arm and a leg, and production cost is big.Therefore small-sized and medium-sized Dairy Processing enterprise can only adopt lower-cost traditional subculture formula ferment agent for sour milk during the production sour milk, owing to reasons such as pollution in the process of going down to posterity and various bacterial classification proportional imbalances cause product quality relatively poor.Microcapsule leaven is the once innovation of leavening, uses natural or synthetic macromolecular material that lactic acid bacteria is wrapped in the microencapsulation of semi permeability or sealing cyst membrane, and under proper condition, lactic acid bacteria discharges.Adopt microcapsules technology that the lactic acid bacteria thalline is carried out embedding, strengthened the thalline resistivity of environmental factor to external world, significantly improve the survival rate of thalline behind low temperature storage life and arrival enteron aisle, make lactic acid bacteria play wholesome effect better.The production that microcapsule leaven is used for fermented milk products is of far-reaching significance.In processes such as the storage of microcapsule leaven, transportation, sale, exist the thalline in the capsule to reduce gradually, capsule intensity descends even broken problem, so that reduces the leavening quality, influences the food fermentation effect.How preserving the capsule leavening, to make thalline keep the maximum viable bacteria amount and the intensity of capsule be assistant officer's problem to be solved, and research at present yet there are no the people and studies.The present invention is directed to this problem and further investigate, develop a kind of protection liquid for storage that can effectively protect the capsule leavening.
Three, summary of the invention
Technical problem the objective of the invention is to develop a kind of protection liquid for storage of microcapsule leaven at the problem that the viable bacteria amount descends and capsule intensity descends in the capsule leavening in the storage of capsule leavening, transportation and the sales process.Microcapsule leaven can effectively reduce the decline of viable bacteria amount and intensity in this protection liquid for storage, the useful life of prolongation leavening fundamentally solves the storage problem of microcapsule leaven, is a kind of protection liquid for storage of microcapsule leaven efficiently.
Technical scheme
The present invention is a kind of protection liquid for storage of microcapsule leaven efficiently, and by sodium chloride, glucose sugar, sucrose, glycerine and calcium carbonate are formed.Its optimum formula ratio is sodium chloride 0.9%, glucose sugar 3%, sucrose 5%, glycerine 10%, calcium carbonate 0.2%.
Beneficial effect
The present invention is directed in the processes such as storage at microcapsule leaven, transportation, sale, the thalline in the capsule can reduce gradually, so that reduces the leavening quality, influences the food fermentation effect.In protection liquid for storage of the present invention, can effectively protect microcapsule leaven, prolong the storage and the useful life of leavening.Improve the international competitiveness of China's capsule leavening product, promote the sustainable development of fermented food industry, increase the contribution of food processing industry national economy, social development.Dairy production industry is then had big meaning, can improve the scientific and technological level that China's fermented dairy product is produced, strengthen the enterprise technology original creativity, increase technology content, optimize product structure, the existing Dairy Production technology of improvement reduces production costs.
Major advantage of the present invention and good effect are as follows:
1, microcapsule leaven can effectively reduce the decline of number of viable in the capsule and the reduction of capsule intensity, the useful life that has prolonged highdensity microcapsule leaven in protection liquid for storage of the present invention.
2, this storage protection formula of liquid is simple, and cost is lower, and all components can eat safely.
3, the invention of this prescription can improve the international competitiveness of China's leavening, and can promote the sustainable development of fermented food industry.
Four, description of drawings
The viable count of Fig. 1 capsule leavening in protection liquid for storage and sterilized water and the change curve of capsule intensity
Cell density in the ■ storage liquid; Cell density in the sterilized water; Capsule intensity in the ▲ storage liquid; Capsule intensity in the △ sterilized water
Five, the specific embodiment
On protectant basis of using when free thalline being preserved according to forefathers; and in conjunction with the result of this problem to the capsule intensity Study on influencing factors; selecting physiological saline for use is the protection liquid on basis; add some sugars therein; glycerine and calcium carbonate; at first carry out experiment of single factor; based on physiological saline; add the glucose (1% of different content therein; 3%; 5%; 7%; 9%); sucrose (1%; 3%; 5%; 7%; 9%); glycerine (5%; 10%; 15%; 20%) and calcium carbonate (0.05%; 0.1%; 0.2%; 0.3%; 0.4%), studies of the influence of these factors to thalline activity and capsule intensity in the capsule.According to design preparation protection liquid for storage; 121 ℃ of sterilizations are after 20 minutes; the high density microcapsule leaven for preparing is put into protection liquid for storage to be preserved one month under 4 ℃ of conditions; adopt the broken capsule method of chemistry to carry out the capsule fragmentation; then by the thalline quantity in the flat band method calculating capsule; capsule intensity adopts positive pressing to measure, and changes the concentration of the best protection effect of determining each factor by cell concentration and capsule intensity.Single factor result of study shows; the interpolation of glucose can have protective effect to the thalline activity, but the variation of concentration is little to the influence of thalline active protection, and is little to the capsule intensity influence; from reducing cost and to the comprehensive angle of thalline protective effect, the concentration of selecting glucose for use is 3%.The variation of the concentration of sucrose, glycerine and calcium carbonate has considerable influence to the variation of cell density and capsule intensity.The experiment of single factor result has shown that also the preferred concentration of sucrose is 5%, and the preferred concentration of glycerine is 15%, and the preferred concentration of calcium carbonate is 0.2%.On the basis of single factor analysis, select sucrose, glycerine and three factors of calcium carbonate for use, design 3 factors, 3 horizontal quadratures test L
9(3
3); the high density microcapsule leaven for preparing put into by the protection liquid for storage of orthogonal design under 4 ℃ of conditions, preserved one month; adopt the broken capsule method of chemistry to carry out the capsule fragmentation; calculate thalline quantity in every gram capsule by flat band method then; adopt positive pressing to measure capsule intensity; determine best storage liquid prescription by cell concentration and capsule intensity variation with a level difference analysis, concrete experimental design scheme and result are as shown in Table 1 and Table 2.
Table 1L
9(3
3) orthogonal experiment factor and water-glass
Table 2L
9(3
3) orthogonal experiment scheme and result
Learning that from the big I of extreme difference R the principal element that influences cell concentration is a cane sugar content, secondly is glycerine and calcium carbonate content, and the primary and secondary of each factor is A＞B＞C in proper order, and best storage liquid prescription is A
2B
2C
3The principal element that influences capsule intensity is a calcium carbonate content, secondly is glycerine and cane sugar content, and the primary and secondary of each factor is C＞A＞B in proper order, and best storage liquid prescription is A
2B
1C
2Calcium carbonate is the minor effect factor of thalline activity, but is major influence factors to capsule intensity, considers the combined influence of each factor active to thalline in capsule intensity, will reduce cost simultaneously, finally determines that the storage protection formula of liquid of the best is A
2B
1C
2Comprehensive monofactorial experimental result, the end formulation of protection liquid for storage are sodium chloride 0.9%, glucose sugar 3%, sucrose 5%, glycerine 10%, calcium carbonate 0.2%.
With the sub-water of going or staying preparation protection liquid for storage, place 121 ℃ of sterilization 20min according to this prescription, (cell density is 1.6 * 10 with the high density for preparing then
11Cfu/g) microcapsule leaven is put into protection liquid for storage and is preserved under 4 ℃ of conditions, and sterilized water is done storage liquid and compared simultaneously.Around each cell density in the capsule and capsule intensity are detected, Fig. 1 is seen in the variation of cell density and capsule intensity.As can be seen from Figure 1, protection liquid for storage has good protection effect to thalline activity in the capsule, and cell density is 2.4 * 10 in 24 all backs capsules
10Cfu/g, and the cell density of capsule is 3.8 * 10 in the sterilized water
3Cfu/g; It can also be seen that from Fig. 1 protection liquid for storage can effectively slow down the reduction of capsule intensity, after 24 weeks, the capsule intensity in the storage liquid is 2.05N, and the capsule intensity in the sterilized water only is 1.12N.Above result shows that the prescription of capsule storage protection liquid of the present invention has thalline activity in the good protection capsule, and can effectively slow down the reduction of capsule intensity.The present invention can effectively prolong the storage and the useful life of capsule leavening, and improves the international competitiveness of China's capsule leavening product.
Claims (2)
1. a high-efficiency protection liquid for storage of microcapsule leaven prescription is characterized in that, is made up of sodium chloride, glucose, sucrose, glycerine and calcium carbonate.
2. protection formula of liquid according to claim 1, its mass ratio are sodium chloride 0.9%, glucose 3%, sucrose 5%, glycerine 10%, calcium carbonate 0.2%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010101857150A CN101869138B (en) | 2010-05-28 | 2010-05-28 | High-efficiency protection liquid for storage of microcapsule leaven |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010101857150A CN101869138B (en) | 2010-05-28 | 2010-05-28 | High-efficiency protection liquid for storage of microcapsule leaven |
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| CN101869138A true CN101869138A (en) | 2010-10-27 |
| CN101869138B CN101869138B (en) | 2012-05-30 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104313005A (en) * | 2014-10-22 | 2015-01-28 | 江苏省农业科学院 | High-stability lactic acid bacteria microcapsule and preparation method and application thereof |
| CN104818237A (en) * | 2015-05-29 | 2015-08-05 | 北京根来福农业科技有限公司 | Microbial preparation, OPS biological slow-release agent containing microbial preparation and preparation method of microbial preparation |
Citations (5)
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| CN1554741A (en) * | 2003-12-24 | 2004-12-15 | 广东省农业科学院农业生物技术研究所 | Stabilizing post-processing method for beneficial microbial preparation |
| CN1844352A (en) * | 2006-05-12 | 2006-10-11 | 河北农业大学 | Composite protector for high activity bifidobacteria dry powder formulation |
| CN1847390A (en) * | 2006-05-12 | 2006-10-18 | 河北农业大学 | Composite protectant for freeze dried lactobcillus fermenting prepn |
| WO2007128829A2 (en) * | 2006-05-09 | 2007-11-15 | Biotica Technology Limited | 18,21-didesoxymacbecin derivatives for the treatment of cancer |
| CN101691559A (en) * | 2009-10-19 | 2010-04-07 | 李军训 | Vacuum freeze-drying method for probiotic lactobacillus |
-
2010
- 2010-05-28 CN CN2010101857150A patent/CN101869138B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1554741A (en) * | 2003-12-24 | 2004-12-15 | 广东省农业科学院农业生物技术研究所 | Stabilizing post-processing method for beneficial microbial preparation |
| WO2007128829A2 (en) * | 2006-05-09 | 2007-11-15 | Biotica Technology Limited | 18,21-didesoxymacbecin derivatives for the treatment of cancer |
| CN1844352A (en) * | 2006-05-12 | 2006-10-11 | 河北农业大学 | Composite protector for high activity bifidobacteria dry powder formulation |
| CN1847390A (en) * | 2006-05-12 | 2006-10-18 | 河北农业大学 | Composite protectant for freeze dried lactobcillus fermenting prepn |
| CN101691559A (en) * | 2009-10-19 | 2010-04-07 | 李军训 | Vacuum freeze-drying method for probiotic lactobacillus |
Non-Patent Citations (3)
| Title |
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| 《Cryobiology》 20031231 Zdenek hubalek Protectants used in the cryopreservation of microorganisms 第205-229页 1-2 第46卷, 2 * |
| 《乳业科学与技术》 20100501 杜磊 等 乳酸菌冷冻保护剂选择的研究 第119-121 1-2 , 第3期 2 * |
| 《江西农业学报》 20091231 周剑忠 等 液芯微胶囊制备条件优化 第138-141页 1-2 第21卷, 第8期 2 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104313005A (en) * | 2014-10-22 | 2015-01-28 | 江苏省农业科学院 | High-stability lactic acid bacteria microcapsule and preparation method and application thereof |
| CN104818237A (en) * | 2015-05-29 | 2015-08-05 | 北京根来福农业科技有限公司 | Microbial preparation, OPS biological slow-release agent containing microbial preparation and preparation method of microbial preparation |
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| CN101869138B (en) | 2012-05-30 |
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